AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG000322

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG000322 MtrunA17_Chr5g0398971 32.704 159 82 8 4 146 7 156 2.07e-16 77.4
MsaG000322 MtrunA17_Chr2g0318101 33.544 158 93 5 4 155 15 166 1.26e-15 75.5
MsaG000322 MtrunA17_Chr4g0050701 29.747 158 87 6 4 147 11 158 5.36e-15 71.2
MsaG000322 MtrunA17_Chr8g0345731 30.128 156 93 5 2 145 22 173 2.06e-14 69.3
MsaG000322 MtrunA17_Chr4g0047841 35.075 134 76 4 4 132 15 142 2.35e-14 71.6
MsaG000322 MtrunA17_Chr2g0326361 31.056 161 83 6 4 145 14 165 3.61e-14 70.9
MsaG000322 MtrunA17_Chr4g0018391 32.258 155 86 7 4 146 7 154 3.84e-14 70.9
MsaG000322 MtrunA17_Chr7g0263931 32.500 160 82 7 4 145 11 162 5.60e-14 70.5
MsaG000322 MtrunA17_Chr2g0309891 31.646 158 85 8 4 146 6 155 6.97e-14 70.1
MsaG000322 MtrunA17_Chr7g0266701 31.138 167 89 8 1 146 1 162 2.43e-13 67.8
MsaG000322 MtrunA17_Chr3g0109821 31.013 158 86 8 4 146 6 155 3.04e-13 68.9
MsaG000322 MtrunA17_Chr8g0372121 32.258 155 86 7 4 146 6 153 3.19e-13 68.2
MsaG000322 MtrunA17_Chr4g0017921 30.460 174 99 9 4 164 8 172 3.96e-13 68.2
MsaG000322 MtrunA17_Chr3g0139331 31.410 156 89 6 2 146 9 157 3.97e-13 68.2
MsaG000322 MtrunA17_Chr7g0261411 33.333 153 84 7 4 146 10 154 4.21e-13 67.4
MsaG000322 MtrunA17_Chr5g0417001 28.144 167 90 7 2 146 12 170 9.57e-13 67.0
MsaG000322 MtrunA17_Chr8g0384351 31.293 147 79 7 4 147 10 137 1.20e-12 64.7
MsaG000322 MtrunA17_Chr8g0359921 31.210 157 86 6 4 146 14 162 1.71e-12 66.2
MsaG000322 MtrunA17_Chr3g0130101 29.375 160 89 6 2 146 5 155 2.03e-12 65.9
MsaG000322 MtrunA17_Chr8g0361731 27.711 166 93 6 2 146 12 171 2.03e-12 66.2
MsaG000322 MtrunA17_Chr2g0318081 30.573 157 85 7 4 146 16 162 4.88e-12 65.1
MsaG000322 MtrunA17_Chr1g0197351 31.902 163 83 7 4 146 42 196 2.87e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG000322 AT2G24430.2 36.076 158 80 7 2 146 14 163 9.95e-17 78.6
MsaG000322 AT2G24430.1 36.076 158 80 7 2 146 14 163 9.95e-17 78.6
MsaG000322 AT2G27300.1 27.273 209 126 8 4 197 14 211 4.31e-15 73.9
MsaG000322 AT1G71930.2 31.646 158 89 6 4 149 9 159 6.55e-15 73.6
MsaG000322 AT1G71930.1 31.646 158 89 6 4 149 9 159 6.55e-15 73.6
MsaG000322 AT5G39610.1 32.903 155 86 6 4 146 20 168 7.95e-15 72.8
MsaG000322 AT1G76420.1 32.500 160 81 7 4 147 22 170 1.28e-14 72.8
MsaG000322 AT1G01720.1 31.056 161 84 6 4 147 7 157 1.60e-14 72.0
MsaG000322 AT1G26870.1 31.613 155 91 5 2 149 14 160 2.24e-14 72.4
MsaG000322 AT4G17980.1 31.646 158 86 6 4 145 6 157 3.38e-14 70.9
MsaG000322 AT4G17980.2 31.646 158 86 6 4 145 6 157 5.88e-14 70.5
MsaG000322 AT5G66300.1 30.323 155 90 6 4 146 12 160 1.11e-13 69.7
MsaG000322 AT3G15500.1 32.484 157 82 8 4 146 14 160 1.25e-13 69.7
MsaG000322 AT3G29035.1 30.968 155 89 5 4 146 24 172 1.76e-13 69.3
MsaG000322 AT5G22290.1 27.320 194 110 8 6 179 23 205 1.91e-13 69.3
MsaG000322 AT4G10350.1 30.387 181 94 10 4 168 9 173 2.10e-13 69.3
MsaG000322 AT5G08790.1 32.099 162 82 7 4 147 7 158 2.19e-13 68.6
MsaG000322 AT4G35580.3 30.675 163 91 8 1 149 6 160 2.21e-13 69.7
MsaG000322 AT4G35580.2 30.675 163 91 8 1 149 6 160 2.21e-13 69.7
MsaG000322 AT4G35580.1 30.675 163 91 8 1 149 6 160 2.50e-13 69.3
MsaG000322 AT5G17260.1 31.013 158 86 7 4 146 6 155 3.24e-13 68.9
MsaG000322 AT3G44290.2 26.846 149 89 6 6 145 16 153 3.34e-13 67.4
MsaG000322 AT1G52890.1 32.278 158 81 8 4 146 14 160 4.54e-13 68.2
MsaG000322 AT5G63790.1 32.515 163 80 8 4 147 50 201 5.70e-13 67.8
MsaG000322 AT5G63790.2 32.515 163 80 8 4 147 60 211 6.01e-13 67.8
MsaG000322 AT1G54330.1 31.818 154 86 7 4 146 6 151 6.98e-13 67.4
MsaG000322 AT3G44290.1 26.846 149 89 6 6 145 16 153 9.12e-13 67.4
MsaG000322 AT1G77450.1 31.875 160 83 7 4 147 10 159 1.24e-12 66.2
MsaG000322 AT4G27410.2 30.128 156 87 6 4 146 14 160 2.31e-12 65.9
MsaG000322 AT2G43000.1 28.289 152 92 5 6 146 20 165 2.64e-12 65.5
MsaG000322 AT3G15510.1 31.214 173 106 6 10 175 23 189 2.90e-12 65.9
MsaG000322 AT1G32510.1 29.268 164 88 7 3 145 5 161 4.45e-12 65.1
MsaG000322 AT5G13180.1 28.947 152 92 5 4 146 14 158 6.19e-12 64.3
MsaG000322 AT3G03200.1 31.013 158 86 7 4 146 6 155 6.41e-12 65.5
MsaG000322 AT5G46590.1 30.625 160 83 8 4 145 6 155 7.01e-12 64.3

Find 50 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCAAATTATGATTTCAATTA+TGG 0.135183 1:-4304403 MsaT000322.1:CDS
CAATGGTTCTGACCTGATTC+AGG 0.237830 1:-4305591 MsaT000322.1:CDS
GGCACTGTAAGACGGGATTT+TGG 0.254019 1:-4305457 MsaT000322.1:CDS
CCTTGGGTGACGGAAGAAAA+AGG 0.350861 1:+4305520 None:intergenic
TTGGATCTGGATGCTGAGTT+TGG 0.378607 1:-4305128 MsaT000322.1:CDS
ACAGGTTCAGCTCCTGAATC+AGG 0.430846 1:+4305579 None:intergenic
ATTGAAATCATAATTTGCTC+AGG 0.450777 1:+4304408 None:intergenic
CCTCATCTGCTCATCAACTT+TGG 0.458061 1:+4305478 None:intergenic
GAAGAGGCTCAGAATTTCAA+TGG 0.469135 1:-4305608 MsaT000322.1:CDS
GCAATTCAAACGGATCATAC+TGG 0.470273 1:+4305555 None:intergenic
ACTCCATTCGTCTTATCAGC+AGG 0.475127 1:+4305068 None:intergenic
GTGAAGCAGCAAAATTCCCT+TGG 0.487125 1:+4305503 None:intergenic
CTGCCCACCAGAGTTGGATC+TGG 0.490020 1:-4305141 MsaT000322.1:CDS
TGGAGTTACTCAGAAACCTA+TGG 0.493323 1:-4305051 MsaT000322.1:CDS
GGAGTAAAACGCAATTCAAA+CGG 0.503611 1:+4305545 None:intergenic
AGCAAAATTCCCTTGGGTGA+CGG 0.508819 1:+4305510 None:intergenic
TTTGGAGCTATAGTTGATGA+AGG 0.517556 1:-4305110 MsaT000322.1:CDS
ACTAGTGCTGCTGCTGAGCA+AGG 0.517825 1:-4305008 MsaT000322.1:CDS
CAGATGAGGCACTGTAAGAC+GGG 0.518000 1:-4305464 MsaT000322.1:CDS
CAAACGGATCATACTGGTAC+AGG 0.523566 1:+4305561 None:intergenic
TTGAAATCATAATTTGCTCA+GGG 0.533329 1:+4304409 None:intergenic
AGAATGCCATCAAAACTAGC+TGG 0.534697 1:-4305337 MsaT000322.1:CDS
GCTGTTCGAGACATTACTGT+TGG 0.539490 1:-4305422 MsaT000322.1:CDS
CACACTTGCTAACAATCCTC+AGG 0.550460 1:-4305300 MsaT000322.1:intron
GGAGTTACTCAGAAACCTAT+GGG 0.559803 1:-4305050 MsaT000322.1:CDS
TGAAGCAGCAAAATTCCCTT+GGG 0.560910 1:+4305504 None:intergenic
CCAAAGTTGATGAGCAGATG+AGG 0.562182 1:-4305478 MsaT000322.1:CDS
AAAACAAATAAATTTATACA+AGG 0.563103 1:+4304355 None:intergenic
TGGCAAGGTAGTGGCTAACA+AGG 0.563537 1:+4305642 None:intergenic
GCATCCAGATCCAACTCTGG+TGG 0.565861 1:+4305137 None:intergenic
GTTTGACTGCCCACCAGAGT+TGG 0.567918 1:-4305147 MsaT000322.1:CDS
CTAACAAGGAGCTCATCAGA+AGG 0.575725 1:+4305656 None:intergenic
TGACTATGCTGTCTTGCACA+TGG 0.580894 1:-4304442 MsaT000322.1:CDS
TAACAAGGAGCTCATCAGAA+GGG 0.587558 1:+4305657 None:intergenic
GACCCTGCTGATAAGACGAA+TGG 0.589128 1:-4305071 MsaT000322.1:CDS
AGTGCTGCTGCTGAGCAAGG+CGG 0.590822 1:-4305005 MsaT000322.1:CDS
GCAGATGAGGCACTGTAAGA+CGG 0.594345 1:-4305465 MsaT000322.1:CDS
TCAGCATCCAGATCCAACTC+TGG 0.601777 1:+4305134 None:intergenic
GCAATGGAATTGCTTCCACC+TGG 0.606391 1:-4305692 MsaT000322.1:CDS
CTCCATTCGTCTTATCAGCA+GGG 0.617597 1:+4305069 None:intergenic
AACAAGGAGCTCATCAGAAG+GGG 0.621007 1:+4305658 None:intergenic
CATCCAGATCCAACTCTGGT+GGG 0.645528 1:+4305138 None:intergenic
GGCACTGATAACATGTTGGT+GGG 0.647357 1:-4305401 MsaT000322.1:CDS
TGGCACTGATAACATGTTGG+TGG 0.649813 1:-4305402 MsaT000322.1:CDS
TGTTGGCACTGATAACATGT+TGG 0.654775 1:-4305405 MsaT000322.1:CDS
GAAGGGGCGAAGTCAACACC+AGG 0.655180 1:+4305674 None:intergenic
TGAAATCATAATTTGCTCAG+GGG 0.667052 1:+4304410 None:intergenic
GGGGCGAAGTCAACACCAGG+TGG 0.667873 1:+4305677 None:intergenic
GAGTTACTCAGAAACCTATG+GGG 0.687052 1:-4305049 MsaT000322.1:CDS
AGTTACTCAGAAACCTATGG+GGG 0.734801 1:-4305048 MsaT000322.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr1 gene 4304368 4305711 4304368 ID=MsaG000322
Chr1 mRNA 4304368 4305711 4304368 ID=MsaT000322.1;Parent=MsaG000322
Chr1 exon 4304368 4304484 4304368 ID=MsaT000322.1.exon3;Parent=MsaT000322.1
Chr1 CDS 4304368 4304484 4304368 ID=cds.MsaT000322.1;Parent=MsaT000322.1
Chr1 exon 4304968 4305192 4304968 ID=MsaT000322.1.exon2;Parent=MsaT000322.1
Chr1 CDS 4304968 4305192 4304968 ID=cds.MsaT000322.1;Parent=MsaT000322.1
Chr1 exon 4305301 4305711 4305301 ID=MsaT000322.1.exon1;Parent=MsaT000322.1
Chr1 CDS 4305301 4305711 4305301 ID=cds.MsaT000322.1;Parent=MsaT000322.1
Gene Sequence

>MsaT000322.1

ATGGAATTGCTTCCACCTGGTGTTGACTTCGCCCCTTCTGATGAGCTCCTTGTTAGCCACTACCTTGCCAACAAAAACAATGAAGAGGCTCAGAATTTCAATGGTTCTGACCTGATTCAGGAGCTGAACCTGTACCAGTATGATCCGTTTGAATTGCGTTTTACTCCGTCCTTTTTCTTCCGTCACCCAAGGGAATTTTGCTGCTTCACTGCCAAAGTTGATGAGCAGATGAGGCACTGTAAGACGGGATTTTGGATGCAAAAGGGGGCTGTTCGAGACATTACTGTTGGCACTGATAACATGTTGGTGGGAAAGAAGTCTCTTTTTATGTTCTATATAGGAAATTCCGCTCAGAATGCCATCAAAACTAGCTGGACTATGTACGAGTACACACTTGCTAACAATCCTCAGGCTCGTTTTGTTGTTTGTCGTGTGTTTGACTGCCCACCAGAGTTGGATCTGGATGCTGAGTTTGGAGCTATAGTTGATGAAGGCAATGCTGCTAGAGATGACCCTGCTGATAAGACGAATGGAGTTACTCAGAAACCTATGGGGGCTGGGGATGGGGCTGGTACTAGTGCTGCTGCTGAGCAAGGCGGTGCTTCAAATGTCACAGATTTTGAAGGCACTCTGCAGGCGAGTTATGCAACATTTCTTGACTATGCTGTCTTGCACATGGAAGCTATGACCCCTGAGCAAATTATGATTTCAATTATGGATGAAGATTTTCTTGAGATTGCAGACCTTGTATAA
Protein sequence

>MsaT000322.1

MELLPPGVDFAPSDELLVSHYLANKNNEEAQNFNGSDLIQELNLYQYDPFELRFTPSFFFRHPREFCCFTAKVDEQMRHCKTGFWMQKGAVRDITVGTDNMLVGKKSLFMFYIGNSAQNAIKTSWTMYEYTLANNPQARFVVCRVFDCPPELDLDAEFGAIVDEGNAARDDPADKTNGVTQKPMGAGDGAGTSAAAEQGGASNVTDFEGTLQASYATFLDYAVLHMEAMTPEQIMISIMDEDFLEIADLV*