AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG000325


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG000325 MtrunA17_Chr1g0153161 97.087 412 12 0 1 412 1 412 0.0 828
MsaG000325 MtrunA17_Chr8g0372101 52.740 146 67 2 1 145 2 146 4.49e-38 144
MsaG000325 MtrunA17_Chr8g0372101 46.000 100 51 2 307 406 461 557 3.10e-18 86.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG000325 AT1G19860.1 43.207 449 179 16 1 409 1 413 7.36e-95 291
MsaG000325 AT1G19860.2 42.424 429 173 15 21 409 21 415 1.89e-85 266
MsaG000325 AT5G66270.1 27.193 456 223 15 6 386 6 427 2.63e-31 124
MsaG000325 AT3G51180.1 34.921 252 125 9 1 225 1 240 3.80e-25 107

Find 95 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCATCTTCAACAGGCGTAAT+TGG 0.151616 1:-4357561 None:intergenic
AGAATATTATTGAGTAATTC+AGG 0.252544 1:-4357729 None:intergenic
TTGCTGGTTCTGGCGATTAC+TGG 0.255064 1:-4358105 None:intergenic
GCAATAGCTGGACTGATTTC+AGG 0.255929 1:-4357981 None:intergenic
TACATTATCTCCTGGTATAT+TGG 0.266652 1:+4357599 MsaT000325.1:CDS
CCGAAATACCAGTTATTAAC+TGG 0.269344 1:+4356891 MsaT000325.1:CDS
TCGTCAACATCCACTGAAAT+AGG 0.270410 1:-4357504 None:intergenic
TTGATAATGGTTGCTGGTTC+TGG 0.279964 1:-4358115 None:intergenic
AGTGGTCTCAACTTGATTAA+AGG 0.289882 1:-4357862 None:intergenic
GACGATTCCCATTACATTGA+TGG 0.304640 1:+4357522 MsaT000325.1:CDS
TAGTTCAAAGAGAATGAAAA+TGG 0.338707 1:+4358304 MsaT000325.1:CDS
TGACGAGGGATTACCTTGTT+GGG 0.359371 1:-4358273 None:intergenic
GGTTTAATCCAGTTAATAAC+TGG 0.362663 1:-4356899 None:intergenic
TTTGTTGAAGGGACCTCAAC+AGG 0.365719 1:-4358011 None:intergenic
CCAGTTAATAACTGGTATTT+CGG 0.370025 1:-4356891 None:intergenic
GTGGTCTCAACTTGATTAAA+GGG 0.370543 1:-4357861 None:intergenic
CTGACGAGGGATTACCTTGT+TGG 0.373906 1:-4358274 None:intergenic
TCAACTTGATTAAAGGGATT+AGG 0.374101 1:-4357855 None:intergenic
ACCAACTTCTCAATCACTTC+CGG 0.375302 1:-4357759 None:intergenic
CATAGTTTGATAATGGTTGC+TGG 0.387584 1:-4358121 None:intergenic
CAACAAGGTAATCCCTCGTC+AGG 0.403842 1:+4358275 MsaT000325.1:CDS
AACATGACCATCAATGTAAT+GGG 0.405673 1:-4357529 None:intergenic
AGCTCCATTCCGACATCCTC+TGG 0.406929 1:-4358216 None:intergenic
CATCTTCAACAGGCGTAATT+GGG 0.418349 1:-4357560 None:intergenic
AGGAATCTCCTTCACAAGTT+GGG 0.421056 1:+4356738 MsaT000325.1:CDS
CAACATGACCATCAATGTAA+TGG 0.430043 1:-4357530 None:intergenic
TGGCGATTACTGGAGGAGTA+TGG 0.439812 1:-4358095 None:intergenic
GAAAATCGAAGAGAGTTTCA+TGG 0.443092 1:+4353691 MsaT000325.1:CDS
TCCGGAAGTGATTGAGAAGT+TGG 0.446609 1:+4357758 MsaT000325.1:CDS
CCAATTACGCCTGTTGAAGA+TGG 0.451403 1:+4357561 MsaT000325.1:CDS
CCCACATCCCCATCTTCAAC+AGG 0.453414 1:-4357570 None:intergenic
GAGGAATCTCCTTCACAAGT+TGG 0.461742 1:+4356737 MsaT000325.1:CDS
CTCACATGGCAAGTGGTGGC+TGG 0.478796 1:+4357297 MsaT000325.1:CDS
ACAGGAGTAGCAGCAATAGC+TGG 0.488249 1:-4357993 None:intergenic
CAATTACGCCTGTTGAAGAT+GGG 0.490357 1:+4357562 MsaT000325.1:CDS
TTCCGAGTTAGATCTTTGTC+AGG 0.491528 1:+4353716 MsaT000325.1:CDS
CAATAATATTCTCAACAATC+CGG 0.493677 1:+4357740 MsaT000325.1:CDS
TTCGTGGATAAATAGCTTCA+AGG 0.494743 1:-4357368 None:intergenic
TTGTTGCCTCAAGGAGAAGC+TGG 0.494944 1:+4356800 MsaT000325.1:CDS
ACTGTATCATAGTTTGATAA+TGG 0.503276 1:-4358128 None:intergenic
TTATCTCCTGGTATATTGGA+TGG 0.518348 1:+4357603 MsaT000325.1:CDS
ACCTCCGGCTGTGTGAGTAT+AGG 0.526743 1:-4357919 None:intergenic
GCATGAGTTCCCTCAAATCC+AGG 0.528859 1:-4356842 None:intergenic
AGACTCGTACTTTGGAGGAA+TGG 0.532082 1:-4357397 None:intergenic
TAACTGGATTAAACCACCTA+AGG 0.535530 1:+4356907 MsaT000325.1:CDS
AGAGAAGACTCGTACTTTGG+AGG 0.538641 1:-4357402 None:intergenic
GACGAGGGATTACCTTGTTG+GGG 0.547183 1:-4358272 None:intergenic
ACAAGGATGATATCTTTGCT+TGG 0.550411 1:-4356781 None:intergenic
GCCTGTTGAAGATGGGGATG+TGG 0.558484 1:+4357569 MsaT000325.1:CDS
TCACACAGCCGGAGGTCAGA+TGG 0.558835 1:+4357926 MsaT000325.1:CDS
GGAGGAATGGAAGATATTCG+TGG 0.560244 1:-4357384 None:intergenic
TGAAGATGGGGATGTGGGAG+CGG 0.563372 1:+4357575 MsaT000325.1:CDS
GGTTAGATTATTCTTATCTG+AGG 0.564458 1:+4356718 MsaT000325.1:intron
CGTCAACTATGTCTACTCAA+TGG 0.565260 1:+4357949 MsaT000325.1:CDS
AGCTTCTCCTTGAGGCAACA+AGG 0.565922 1:-4356798 None:intergenic
GGAGCGGATACATTATCTCC+TGG 0.567885 1:+4357591 MsaT000325.1:CDS
ACTTGATTAAAGGGATTAGG+AGG 0.568417 1:-4357852 None:intergenic
TATTGCTGCTACTCCTGTTG+AGG 0.569271 1:+4357998 MsaT000325.1:CDS
TTAATAGTTCCAGAGGATGT+CGG 0.569456 1:+4358207 MsaT000325.1:CDS
TGTCTACTCAATGGCATCGT+AGG 0.571059 1:+4357958 MsaT000325.1:CDS
CTGGTTCTGGCGATTACTGG+AGG 0.572380 1:-4358102 None:intergenic
AGTATTTGATGAGGATGTAG+TGG 0.572858 1:-4357880 None:intergenic
GATATTTGATCAACATCAGA+GGG 0.578943 1:+4357335 MsaT000325.1:CDS
GAATTCAACCCAACTTGTGA+AGG 0.578955 1:-4356746 None:intergenic
CACTGTTACGACTATGTCGA+TGG 0.580449 1:+4357632 MsaT000325.1:CDS
AGGATGATATCTTTGCTTGG+AGG 0.580593 1:-4356778 None:intergenic
ATATCATCCTTGTTGCCTCA+AGG 0.583248 1:+4356791 MsaT000325.1:CDS
TCTTCCTATACTCACACAGC+CGG 0.583250 1:+4357915 MsaT000325.1:CDS
TCGATGCAGTCCTATTTCAG+TGG 0.588300 1:+4357494 MsaT000325.1:intron
TTAATAACTGGTATTTCGGA+CGG 0.588985 1:-4356887 None:intergenic
TGAACTATGCATCCCTGACG+AGG 0.599293 1:-4358288 None:intergenic
AAGAGCTTGATTCAGCAACA+TGG 0.600253 1:+4358050 MsaT000325.1:CDS
TCAGAACCAGCTTCTCCTTG+AGG 0.604428 1:-4356806 None:intergenic
AGTTCCAGAGGATGTCGGAA+TGG 0.612631 1:+4358212 MsaT000325.1:CDS
AGTTGACGCCATCTGACCTC+CGG 0.616959 1:-4357934 None:intergenic
TACCTGACAAAGATCTAACT+CGG 0.627396 1:-4353718 None:intergenic
TTTGAACCATCCAATATACC+AGG 0.631062 1:-4357609 None:intergenic
GAACTATGCATCCCTGACGA+GGG 0.631209 1:-4358287 None:intergenic
TGACAAAGATCTAACTCGGA+AGG 0.633363 1:-4353714 None:intergenic
CCTGTTGAAGATGGGGATGT+GGG 0.634741 1:+4357570 MsaT000325.1:CDS
GCTAGATCTCACATGGCAAG+TGG 0.640415 1:+4357290 MsaT000325.1:CDS
AAATGATGCATCATGCTGAA+AGG 0.642980 1:-4358246 None:intergenic
GCATCATTTCAGCCCCAACA+AGG 0.644422 1:+4358260 MsaT000325.1:CDS
AGATATTTGATCAACATCAG+AGG 0.644462 1:+4357334 MsaT000325.1:CDS
AGATCTCACATGGCAAGTGG+TGG 0.645516 1:+4357293 MsaT000325.1:CDS
TCCTATACTCACACAGCCGG+AGG 0.650386 1:+4357918 MsaT000325.1:CDS
AGATCGTGCTAGATCTCACA+TGG 0.650447 1:+4357283 MsaT000325.1:intron
TTAGTGAAGAGCAGTGAACA+TGG 0.653142 1:+4357693 MsaT000325.1:CDS
AATTACGCCTGTTGAAGATG+GGG 0.658485 1:+4357563 MsaT000325.1:CDS
AACAAGGTAATCCCTCGTCA+GGG 0.664383 1:+4358276 MsaT000325.1:CDS
GCACAGTATGCACAAAACAC+CGG 0.673863 1:+4357804 MsaT000325.1:CDS
TGTGTGAGTATAGGAAGAAG+TGG 0.682691 1:-4357910 None:intergenic
GCGGTGTGTGAAAATATGCG+TGG 0.706081 1:+4353663 None:intergenic
AGCTTGATTCAGCAACATGG+AGG 0.714964 1:+4358053 MsaT000325.1:CDS
TGAGTTCCCTCAAATCCAGG+AGG 0.756506 1:-4356839 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr1 gene 4353678 4358346 4353678 ID=MsaG000325
Chr1 mRNA 4353678 4358346 4353678 ID=MsaT000325.1;Parent=MsaG000325
Chr1 exon 4353678 4353737 4353678 ID=MsaT000325.1.exon1;Parent=MsaT000325.1
Chr1 CDS 4353678 4353737 4353678 ID=cds.MsaT000325.1;Parent=MsaT000325.1
Chr1 exon 4356719 4356928 4356719 ID=MsaT000325.1.exon2;Parent=MsaT000325.1
Chr1 CDS 4356719 4356928 4356719 ID=cds.MsaT000325.1;Parent=MsaT000325.1
Chr1 exon 4357285 4357409 4357285 ID=MsaT000325.1.exon3;Parent=MsaT000325.1
Chr1 CDS 4357285 4357409 4357285 ID=cds.MsaT000325.1;Parent=MsaT000325.1
Chr1 exon 4357503 4358346 4357503 ID=MsaT000325.1.exon4;Parent=MsaT000325.1
Chr1 CDS 4357503 4358346 4357503 ID=cds.MsaT000325.1;Parent=MsaT000325.1
Gene Sequence

>MsaT000325.1

ATGCGTGGATTGCGAAAATCGAAGAGAGTTTCATGGCCTTCCGAGTTAGATCTTTGTCAGGTTAGATTATTCTTATCTGAGGAATCTCCTTCACAAGTTGGGTTGAATTCTCAAGATCACCTCCAAGCAAAGATATCATCCTTGTTGCCTCAAGGAGAAGCTGGTTCTGATGATATTTTACCTCCTGGATTTGAGGGAACTCATGCTTCAAGTCAGTTTGACATTAAGCCGTCCGAAATACCAGTTATTAACTGGATTAAACCACCTAAGATCGTGCTAGATCTCACATGGCAAGTGGTGGCTGGAGAAGAAAGCAAAGAGATATTTGATCAACATCAGAGGGAGATGAGAGTCCTTGAAGCTATTTATCCACGAATATCTTCCATTCCTCCAAATCCTATTTCAGTGGATGTTGACGATTCCCATTACATTGATGGTCATGTTGTCGTGATCCCAATTACGCCTGTTGAAGATGGGGATGTGGGAGCGGATACATTATCTCCTGGTATATTGGATGGTTCAAACACTGTTACGACTATGTCGATGGTGTCTGATGTAGCTGCTGCATCTGTTGCTTTAACTAGCTTAGTGAAGAGCAGTGAACATGGTAATTTGATTGATCCTGAATTACTCAATAATATTCTCAACAATCCGGAAGTGATTGAGAAGTTGGTTAGAAATTATAATGTTCAAAATGCACAGTATGCACAAAACACCGGATCATCATCAAATGTTTTTTCACATCCTCCTAATCCCTTTAATCAAGTTGAGACCACTACATCCTCATCAAATACTTTTTCATCCACTTCTTCCTATACTCACACAGCCGGAGGTCAGATGGCGTCAACTATGTCTACTCAATGGCATCGTAGGCCTGAAATCAGTCCAGCTATTGCTGCTACTCCTGTTGAGGTCCCTTCAACAAAAGATGTAAACTACTATAAGAGCTTGATTCAGCAACATGGAGGAGATAAACAAGAAGCACTTCCATACTCCTCCAGTAATCGCCAGAACCAGCAACCATTATCAAACTATGATACAGTACACAATTTTAGAGCAAAAGGATCAAAACCAAAAACGACGAAGCCTTGTATCTTTTTTAATAGTTCCAGAGGATGTCGGAATGGAGCTAACTGTACCTTTCAGCATGATGCATCATTTCAGCCCCAACAAGGTAATCCCTCGTCAGGGATGCATAGTTCAAAGAGAATGAAAATGGATAATGAGATCAGCAGTTAA

Protein sequence

>MsaT000325.1

MRGLRKSKRVSWPSELDLCQVRLFLSEESPSQVGLNSQDHLQAKISSLLPQGEAGSDDILPPGFEGTHASSQFDIKPSEIPVINWIKPPKIVLDLTWQVVAGEESKEIFDQHQREMRVLEAIYPRISSIPPNPISVDVDDSHYIDGHVVVIPITPVEDGDVGADTLSPGILDGSNTVTTMSMVSDVAAASVALTSLVKSSEHGNLIDPELLNNILNNPEVIEKLVRNYNVQNAQYAQNTGSSSNVFSHPPNPFNQVETTTSSSNTFSSTSSYTHTAGGQMASTMSTQWHRRPEISPAIAATPVEVPSTKDVNYYKSLIQQHGGDKQEALPYSSSNRQNQQPLSNYDTVHNFRAKGSKPKTTKPCIFFNSSRGCRNGANCTFQHDASFQPQQGNPSSGMHSSKRMKMDNEISS*