Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG000325 | XP_003589157.1 | 97.087 | 412 | 12 | 0 | 1 | 412 | 1 | 412 | 0.0 | 828 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG000325 | sp|Q56XU4|C3H6_ARATH | 43.207 | 449 | 179 | 16 | 1 | 409 | 1 | 413 | 7.24e-94 | 291 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsaG000135 | MsaG000325 | 0.848538 | 1.089121e-59 | 5.615645e-57 |
| MsaG000139 | MsaG000325 | 0.823425 | 2.464150e-53 | 5.938451e-51 |
| MsaG000142 | MsaG000325 | 0.862382 | 1.043627e-63 | 8.889585e-61 |
| MsaG000242 | MsaG000325 | 0.809308 | 3.443805e-50 | 5.783253e-48 |
| MsaG000325 | MsaG000408 | 0.819704 | 1.767071e-52 | 3.855953e-50 |
| MsaG000325 | MsaG000419 | 0.811110 | 1.413351e-50 | 2.479886e-48 |
| MsaG000325 | MsaG000459 | 0.833992 | 7.043967e-56 | 2.289341e-53 |
| MsaG000325 | MsaG000638 | 0.807764 | 7.326796e-50 | 1.185610e-47 |
| MsaG000325 | MsaG000613 | 0.805519 | 2.171660e-49 | 3.332208e-47 |
| MsaG000325 | MsaG000648 | 0.817241 | 6.350803e-52 | 1.299957e-49 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG000325 | MtrunA17_Chr1g0153161 | 97.087 | 412 | 12 | 0 | 1 | 412 | 1 | 412 | 0.0 | 828 |
| MsaG000325 | MtrunA17_Chr8g0372101 | 52.740 | 146 | 67 | 2 | 1 | 145 | 2 | 146 | 4.49e-38 | 144 |
| MsaG000325 | MtrunA17_Chr8g0372101 | 46.000 | 100 | 51 | 2 | 307 | 406 | 461 | 557 | 3.10e-18 | 86.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG000325 | AT1G19860.1 | 43.207 | 449 | 179 | 16 | 1 | 409 | 1 | 413 | 7.36e-95 | 291 |
| MsaG000325 | AT1G19860.2 | 42.424 | 429 | 173 | 15 | 21 | 409 | 21 | 415 | 1.89e-85 | 266 |
| MsaG000325 | AT5G66270.1 | 27.193 | 456 | 223 | 15 | 6 | 386 | 6 | 427 | 2.63e-31 | 124 |
| MsaG000325 | AT3G51180.1 | 34.921 | 252 | 125 | 9 | 1 | 225 | 1 | 240 | 3.80e-25 | 107 |
Find 95 sgRNAs with CRISPR-Local
Find sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAATGATGCATCATGCTGAA+AGG | 0.642980 | 1:-4358246 | None:intergenic |
| AACAAGGTAATCCCTCGTCA+GGG | 0.664383 | 1:+4358276 | MsaT000325.1:CDS |
| AACATGACCATCAATGTAAT+GGG | 0.405673 | 1:-4357529 | None:intergenic |
| AAGAGCTTGATTCAGCAACA+TGG | 0.600253 | 1:+4358050 | MsaT000325.1:CDS |
| AATTACGCCTGTTGAAGATG+GGG | 0.658485 | 1:+4357563 | MsaT000325.1:CDS |
| ACAAGGATGATATCTTTGCT+TGG | 0.550411 | 1:-4356781 | None:intergenic |
| ACAGGAGTAGCAGCAATAGC+TGG | 0.488249 | 1:-4357993 | None:intergenic |
| ACCAACTTCTCAATCACTTC+CGG | 0.375302 | 1:-4357759 | None:intergenic |
| ACCTCCGGCTGTGTGAGTAT+AGG | 0.526743 | 1:-4357919 | None:intergenic |
| ACTGTATCATAGTTTGATAA+TGG | 0.503276 | 1:-4358128 | None:intergenic |
CRISPR-GE
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr1 | gene | 4353678 | 4358346 | 4353678 | ID=MsaG000325 |
| Chr1 | mRNA | 4353678 | 4358346 | 4353678 | ID=MsaT000325.1;Parent=MsaG000325 |
| Chr1 | exon | 4353678 | 4353737 | 4353678 | ID=MsaT000325.1.exon1;Parent=MsaT000325.1 |
| Chr1 | CDS | 4353678 | 4353737 | 4353678 | ID=cds.MsaT000325.1;Parent=MsaT000325.1 |
| Chr1 | exon | 4356719 | 4356928 | 4356719 | ID=MsaT000325.1.exon2;Parent=MsaT000325.1 |
| Chr1 | CDS | 4356719 | 4356928 | 4356719 | ID=cds.MsaT000325.1;Parent=MsaT000325.1 |
| Chr1 | exon | 4357285 | 4357409 | 4357285 | ID=MsaT000325.1.exon3;Parent=MsaT000325.1 |
| Chr1 | CDS | 4357285 | 4357409 | 4357285 | ID=cds.MsaT000325.1;Parent=MsaT000325.1 |
| Chr1 | exon | 4357503 | 4358346 | 4357503 | ID=MsaT000325.1.exon4;Parent=MsaT000325.1 |
| Chr1 | CDS | 4357503 | 4358346 | 4357503 | ID=cds.MsaT000325.1;Parent=MsaT000325.1 |
| Gene Sequence |
>MsaT000325.1 ATGCGTGGATTGCGAAAATCGAAGAGAGTTTCATGGCCTTCCGAGTTAGATCTTTGTCAGGTTAGATTATTCTTATCTGAGGAATCTCCTTCACAAGTTGGGTTGAATTCTCAAGATCACCTCCAAGCAAAGATATCATCCTTGTTGCCTCAAGGAGAAGCTGGTTCTGATGATATTTTACCTCCTGGATTTGAGGGAACTCATGCTTCAAGTCAGTTTGACATTAAGCCGTCCGAAATACCAGTTATTAACTGGATTAAACCACCTAAGATCGTGCTAGATCTCACATGGCAAGTGGTGGCTGGAGAAGAAAGCAAAGAGATATTTGATCAACATCAGAGGGAGATGAGAGTCCTTGAAGCTATTTATCCACGAATATCTTCCATTCCTCCAAATCCTATTTCAGTGGATGTTGACGATTCCCATTACATTGATGGTCATGTTGTCGTGATCCCAATTACGCCTGTTGAAGATGGGGATGTGGGAGCGGATACATTATCTCCTGGTATATTGGATGGTTCAAACACTGTTACGACTATGTCGATGGTGTCTGATGTAGCTGCTGCATCTGTTGCTTTAACTAGCTTAGTGAAGAGCAGTGAACATGGTAATTTGATTGATCCTGAATTACTCAATAATATTCTCAACAATCCGGAAGTGATTGAGAAGTTGGTTAGAAATTATAATGTTCAAAATGCACAGTATGCACAAAACACCGGATCATCATCAAATGTTTTTTCACATCCTCCTAATCCCTTTAATCAAGTTGAGACCACTACATCCTCATCAAATACTTTTTCATCCACTTCTTCCTATACTCACACAGCCGGAGGTCAGATGGCGTCAACTATGTCTACTCAATGGCATCGTAGGCCTGAAATCAGTCCAGCTATTGCTGCTACTCCTGTTGAGGTCCCTTCAACAAAAGATGTAAACTACTATAAGAGCTTGATTCAGCAACATGGAGGAGATAAACAAGAAGCACTTCCATACTCCTCCAGTAATCGCCAGAACCAGCAACCATTATCAAACTATGATACAGTACACAATTTTAGAGCAAAAGGATCAAAACCAAAAACGACGAAGCCTTGTATCTTTTTTAATAGTTCCAGAGGATGTCGGAATGGAGCTAACTGTACCTTTCAGCATGATGCATCATTTCAGCCCCAACAAGGTAATCCCTCGTCAGGGATGCATAGTTCAAAGAGAATGAAAATGGATAATGAGATCAGCAGTTAA |
| Protein sequence |
>MsaT000325.1 MRGLRKSKRVSWPSELDLCQVRLFLSEESPSQVGLNSQDHLQAKISSLLPQGEAGSDDILPPGFEGTHASSQFDIKPSEIPVINWIKPPKIVLDLTWQVVAGEESKEIFDQHQREMRVLEAIYPRISSIPPNPISVDVDDSHYIDGHVVVIPITPVEDGDVGADTLSPGILDGSNTVTTMSMVSDVAAASVALTSLVKSSEHGNLIDPELLNNILNNPEVIEKLVRNYNVQNAQYAQNTGSSSNVFSHPPNPFNQVETTTSSSNTFSSTSSYTHTAGGQMASTMSTQWHRRPEISPAIAATPVEVPSTKDVNYYKSLIQQHGGDKQEALPYSSSNRQNQQPLSNYDTVHNFRAKGSKPKTTKPCIFFNSSRGCRNGANCTFQHDASFQPQQGNPSSGMHSSKRMKMDNEISS* |
