Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG000371 | XP_024631841.1 | 95.337 | 193 | 7 | 2 | 2 | 194 | 1 | 191 | 1.11e-130 | 376 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG000371 | sp|Q6Z1Y6|SNAT2_ORYSJ | 60.544 | 147 | 55 | 2 | 43 | 187 | 43 | 188 | 4.28e-60 | 190 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|---|---|
| MsaG000371 | TR | GNAT |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsaG000371 | MsaG002732 | 0.806430 | 1.399696e-49 | 2.194478e-47 |
| MsaG000371 | MsaG008436 | 0.852235 | 1.010314e-60 | 5.917408e-58 |
| MsaG000371 | MsaG008863 | 0.834695 | 4.700201e-56 | 1.560169e-53 |
| MsaG000371 | MsaG010058 | 0.808041 | 6.403961e-50 | 1.043188e-47 |
| MsaG000371 | MsaG010966 | 0.849925 | 4.497774e-60 | 2.431456e-57 |
| MsaG000371 | MsaG012006 | 0.864605 | 2.150629e-64 | 2.000103e-61 |
| MsaG000371 | MsaG013267 | 0.801624 | 1.382823e-48 | 1.939562e-46 |
| MsaG000371 | MsaG016508 | 0.825678 | 7.305146e-54 | 1.872336e-51 |
| MsaG000371 | MsaG018006 | 0.805422 | 2.275108e-49 | 3.483118e-47 |
| MsaG000371 | MsaG018842 | 0.807389 | 8.795460e-50 | 1.410512e-47 |
| MsaG000371 | MsaG026693 | 0.836780 | 1.401935e-56 | 4.953730e-54 |
| MsaG000371 | MsaG027034 | 0.849730 | 5.096416e-60 | 2.736859e-57 |
| MsaG000371 | MsaG030055 | 0.853057 | 5.903996e-61 | 3.559382e-58 |
| MsaG000371 | MsaG030680 | 0.860173 | 4.883786e-63 | 3.822661e-60 |
| MsaG000371 | MsaG034707 | 0.818903 | 2.684389e-52 | 5.735848e-50 |
| MsaG000371 | MsaG035278 | 0.808991 | 4.022804e-50 | 6.704732e-48 |
| MsaG000371 | MsaG045798 | 0.820472 | 1.181297e-52 | 2.630780e-50 |
| MsaG000371 | MsaG027221 | 0.865020 | 1.596891e-64 | 1.509959e-61 |
| MsaG000371 | MsaG033256 | 0.813049 | 5.363542e-51 | 9.872905e-49 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG000371 | MtrunA17_Chr1g0152491 | 95.337 | 193 | 7 | 2 | 2 | 194 | 1 | 191 | 1.02e-134 | 376 |
| MsaG000371 | MtrunA17_Chr7g0245211 | 43.046 | 151 | 73 | 2 | 47 | 187 | 97 | 244 | 6.14e-32 | 116 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG000371 | AT1G26220.1 | 61.194 | 201 | 65 | 5 | 2 | 194 | 1 | 196 | 1.63e-78 | 234 |
| MsaG000371 | AT1G32070.3 | 41.985 | 131 | 60 | 1 | 76 | 190 | 127 | 257 | 8.66e-29 | 108 |
| MsaG000371 | AT1G32070.1 | 40.909 | 132 | 61 | 1 | 76 | 190 | 126 | 257 | 9.42e-28 | 106 |
| MsaG000371 | AT1G32070.2 | 40.909 | 132 | 61 | 1 | 76 | 190 | 127 | 258 | 9.62e-28 | 106 |
Find 55 sgRNAs with CRISPR-Local
Find sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATACTTTGTTAAGTGCTTCT+AGG | 0.216082 | 1:+5045981 | None:intergenic |
| TACTCTGAGCCAAGAGTTCT+TGG | 0.233326 | 1:-5045695 | MsaT000371.1:CDS |
| CAAGAGGAATTTGAGAAATC+TGG | 0.296177 | 1:-5045198 | MsaT000371.1:CDS |
| AACAACAACATCCCATATTA+TGG | 0.310068 | 1:+5045811 | None:intergenic |
| GTTGTTGATCCTTCGTTTCA+AGG | 0.353170 | 1:-5045794 | MsaT000371.1:CDS |
| CTCAGAGTAAAGAGCAATAT+TGG | 0.361910 | 1:+5045709 | None:intergenic |
| GATCCTTCGTTTCAAGGGCT+TGG | 0.366151 | 1:-5045788 | MsaT000371.1:CDS |
| GAAATCTGGGCACATCAAAA+AGG | 0.398298 | 1:-5045184 | MsaT000371.1:CDS |
| TCGTTTCAAGGGCTTGGACT+AGG | 0.403696 | 1:-5045782 | MsaT000371.1:CDS |
| CTGTTGTCGAGAGGCTTATG+AGG | 0.408843 | 1:-5045754 | MsaT000371.1:CDS |
| CAAGCTGCAAACTCAAATAA+TGG | 0.416111 | 1:-5045151 | None:intergenic |
| AAGTTAAGTCGTAGGGAGGA+TGG | 0.419412 | 1:+5046139 | None:intergenic |
| CGTTTCAAGGGCTTGGACTA+GGG | 0.426846 | 1:-5045781 | MsaT000371.1:CDS |
| CGATAAAAGCCAAGAACTCT+TGG | 0.431331 | 1:+5045686 | None:intergenic |
| TAGGCTTGCTTGACAAGAAA+TGG | 0.442809 | 1:+5046081 | None:intergenic |
| TTGTTGATCCTTCGTTTCAA+GGG | 0.483590 | 1:-5045793 | MsaT000371.1:CDS |
| GTTGCATTTGCAAGAGCTAC+TGG | 0.489343 | 1:-5045851 | MsaT000371.1:CDS |
| ATTCTCCGCCGAACCGCAGA+GGG | 0.489832 | 1:-5046010 | MsaT000371.1:CDS |
| GGTTTAACCCCTCTGCGGTT+CGG | 0.494949 | 1:+5046002 | None:intergenic |
| TTTGAAGAAGTTAAGTCGTA+GGG | 0.496530 | 1:+5046132 | None:intergenic |
| AAGAGGAATTTGAGAAATCT+GGG | 0.514312 | 1:-5045197 | MsaT000371.1:CDS |
| TGAGGGATCTTGTAGGAAGA+GGG | 0.520334 | 1:-5045736 | MsaT000371.1:CDS |
| ACTCGAGCACACTGACTCAA+TGG | 0.522170 | 1:-5045909 | MsaT000371.1:CDS |
| ATTTGAAGAAGTTAAGTCGT+AGG | 0.522993 | 1:+5046131 | None:intergenic |
| ATGAGGGATCTTGTAGGAAG+AGG | 0.534453 | 1:-5045737 | MsaT000371.1:CDS |
| AGTCGTAGGGAGGATGGAAG+AGG | 0.540218 | 1:+5046145 | None:intergenic |
| ATATTGAGAGTGGGTAGTCT+AGG | 0.541441 | 1:+5046062 | None:intergenic |
| ACCATTCCACGAATACCATC+AGG | 0.547694 | 1:+5045641 | None:intergenic |
| AGGCTTATGAGGGATCTTGT+AGG | 0.549706 | 1:-5045743 | MsaT000371.1:CDS |
| AGCACACTGACTCAATGGTA+TGG | 0.558828 | 1:-5045904 | MsaT000371.1:CDS |
| ACAAAGTATTCGTAGCAGTA+GGG | 0.561588 | 1:-5045967 | MsaT000371.1:CDS |
| TCCTGATGGTATTCGTGGAA+TGG | 0.563938 | 1:-5045642 | MsaT000371.1:CDS |
| AAATGGTGAGGTTTCGTAGT+GGG | 0.566008 | 1:+5046098 | None:intergenic |
| AACAAAGTATTCGTAGCAGT+AGG | 0.566118 | 1:-5045968 | MsaT000371.1:CDS |
| TTAACCCCTCTGCGGTTCGG+CGG | 0.568840 | 1:+5046005 | None:intergenic |
| GAAGAAGTTAAGTCGTAGGG+AGG | 0.570827 | 1:+5046135 | None:intergenic |
| GCTGATCCTGATGGTATTCG+TGG | 0.578470 | 1:-5045647 | MsaT000371.1:CDS |
| CATTCTCCGCCGAACCGCAG+AGG | 0.581744 | 1:-5046011 | MsaT000371.1:CDS |
| GTTTCGTCGGATATTGAGAG+TGG | 0.593105 | 1:+5046052 | None:intergenic |
| TTGCTTGACAAGAAATGGTG+AGG | 0.596575 | 1:+5046086 | None:intergenic |
| GATAATATGCTGCTCTTACG+TGG | 0.618724 | 1:-5046181 | None:intergenic |
| AGTCCAAGCCCTTGAAACGA+AGG | 0.624386 | 1:+5045785 | None:intergenic |
| TGTTGTCGAGAGGCTTATGA+GGG | 0.625783 | 1:-5045753 | MsaT000371.1:CDS |
| TTTGCAAGAGCTACTGGTGA+TGG | 0.636078 | 1:-5045845 | MsaT000371.1:CDS |
| TTCTAGGTTTAACCCCTCTG+CGG | 0.644451 | 1:+5045997 | None:intergenic |
| TTTCGTCGGATATTGAGAGT+GGG | 0.648674 | 1:+5046053 | None:intergenic |
| ATGTGTTGATTGCAGGCAAG+AGG | 0.650631 | 1:-5045214 | MsaT000371.1:intron |
| TAGGGAAAGCTGTTGTCGAG+AGG | 0.652617 | 1:-5045763 | MsaT000371.1:CDS |
| CAATGGTATGGATACAAGAG+AGG | 0.657246 | 1:-5045892 | MsaT000371.1:CDS |
| TCGTAGCAGTAGGGTTTCCG+CGG | 0.663293 | 1:-5045958 | MsaT000371.1:CDS |
| GGATCAGCAACAAAACCCAA+AGG | 0.674227 | 1:+5045662 | None:intergenic |
| GAGGAAGTCGCAGAATAATG+CGG | 0.702415 | 1:-5045873 | MsaT000371.1:CDS |
| GAAATGGTGAGGTTTCGTAG+TGG | 0.706232 | 1:+5046097 | None:intergenic |
| GACGAAACCCTAGAAAAGCG+AGG | 0.715831 | 1:-5046037 | MsaT000371.1:CDS |
| TTCTCCGCCGAACCGCAGAG+GGG | 0.756624 | 1:-5046009 | MsaT000371.1:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr1 | gene | 5045154 | 5046197 | 5045154 | ID=MsaG000371 |
| Chr1 | mRNA | 5045154 | 5046197 | 5045154 | ID=MsaT000371.1;Parent=MsaG000371 |
| Chr1 | exon | 5045154 | 5045221 | 5045154 | ID=MsaT000371.1.exon2;Parent=MsaT000371.1 |
| Chr1 | CDS | 5045154 | 5045221 | 5045154 | ID=cds.MsaT000371.1;Parent=MsaT000371.1 |
| Chr1 | exon | 5045606 | 5046197 | 5045606 | ID=MsaT000371.1.exon1;Parent=MsaT000371.1 |
| Chr1 | CDS | 5045606 | 5046197 | 5045606 | ID=cds.MsaT000371.1;Parent=MsaT000371.1 |
| Gene Sequence |
| Protein sequence |
