AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG000377


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG000377 MtrunA17_Chr1g0152431 98.817 169 2 0 1 169 1 169 1.08e-125 351
MsaG000377 MtrunA17_Chr1g0152431 42.553 94 51 2 3 96 103 193 1.49e-14 68.2
MsaG000377 MtrunA17_Chr4g0059371 66.667 168 55 1 2 169 3 169 2.96e-83 243
MsaG000377 MtrunA17_Chr4g0059371 45.833 72 39 0 3 74 103 174 2.45e-15 70.1
MsaG000377 MtrunA17_Chr3g0113051 59.064 171 66 2 1 169 1 169 2.43e-68 206
MsaG000377 MtrunA17_Chr3g0113051 47.826 69 36 0 3 71 103 171 2.75e-18 78.2
MsaG000377 MtrunA17_Chr8g0386501 54.386 171 66 4 1 169 1 161 2.56e-58 181
MsaG000377 MtrunA17_Chr8g0386501 41.414 99 54 1 3 101 95 189 3.84e-19 80.9
MsaG000377 MtrunA17_Chr7g0247111 50.888 169 79 2 1 169 1 165 4.46e-58 181
MsaG000377 MtrunA17_Chr7g0247111 46.377 69 37 0 3 71 99 167 2.23e-15 70.9
MsaG000377 MtrunA17_Chr8g0349391 50.888 169 77 2 1 169 1 163 8.51e-58 179
MsaG000377 MtrunA17_Chr8g0349391 53.623 69 32 0 3 71 97 165 7.15e-19 80.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG000377 AT1G10200.1 85.799 169 24 0 1 169 1 169 2.35e-109 310
MsaG000377 AT1G10200.1 44.444 72 40 0 3 74 103 174 7.50e-16 72.0
MsaG000377 AT1G10200.2 82.400 125 22 0 45 169 3 127 1.85e-75 223
MsaG000377 AT1G10200.2 44.444 72 40 0 3 74 61 132 1.47e-16 72.8
MsaG000377 AT2G39900.1 58.480 171 66 2 1 169 1 168 3.50e-67 204
MsaG000377 AT2G39900.1 49.275 69 35 0 3 71 102 170 1.81e-18 79.0
MsaG000377 AT3G55770.3 55.491 173 67 2 1 169 1 167 2.89e-65 199
MsaG000377 AT3G55770.3 50.725 69 34 0 3 71 101 169 1.73e-19 81.6
MsaG000377 AT3G55770.5 55.491 173 67 2 1 169 1 167 2.89e-65 199
MsaG000377 AT3G55770.5 50.725 69 34 0 3 71 101 169 1.73e-19 81.6
MsaG000377 AT3G55770.2 55.491 173 67 2 1 169 1 167 2.89e-65 199
MsaG000377 AT3G55770.2 50.725 69 34 0 3 71 101 169 1.73e-19 81.6
MsaG000377 AT3G55770.1 55.491 173 67 2 1 169 1 167 2.89e-65 199
MsaG000377 AT3G55770.1 50.725 69 34 0 3 71 101 169 1.73e-19 81.6
MsaG000377 AT2G45800.1 52.071 169 77 1 1 169 1 165 4.93e-59 184
MsaG000377 AT2G45800.1 50.725 69 34 0 3 71 99 167 2.20e-16 73.9
MsaG000377 AT1G01780.1 50.296 169 78 2 1 169 1 163 6.31e-58 180
MsaG000377 AT1G01780.1 52.174 69 33 0 3 71 97 165 5.22e-17 75.5
MsaG000377 AT3G55770.7 46.377 207 67 3 1 169 1 201 2.03e-57 180
MsaG000377 AT3G55770.7 33.981 103 34 1 3 71 101 203 1.13e-12 64.3
MsaG000377 AT3G61230.1 50.000 168 80 1 2 169 3 166 2.83e-57 179
MsaG000377 AT3G61230.1 36.792 106 67 0 3 108 100 205 2.05e-17 76.6
MsaG000377 AT3G55770.4 48.201 139 61 3 1 135 1 132 2.65e-40 133
MsaG000377 AT3G55770.4 50.000 66 33 0 103 168 3 68 2.38e-15 69.3
MsaG000377 AT3G55770.6 48.462 130 57 2 1 126 1 124 2.04e-38 128
MsaG000377 AT3G55770.6 50.000 66 33 0 103 168 3 68 1.93e-15 69.7
MsaG000377 AT2G45800.2 47.794 136 66 2 34 169 4 134 5.54e-37 126
MsaG000377 AT2G45800.2 50.725 69 34 0 3 71 68 136 1.09e-16 74.3

Find 35 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCATTATCTATATTTCTTTC+TGG 0.117591 1:-5090696 None:intergenic
TCCCTCATGTGCTATGTAAT+TGG 0.275186 1:-5091252 None:intergenic
AGCATTTGAAACAACTCTTA+TGG 0.319973 1:-5091202 None:intergenic
TTTGCTTGATCAGTTGAATA+TGG 0.339883 1:-5091295 None:intergenic
TTTATAAAGGTTACAGTGAA+TGG 0.352930 1:+5091170 MsaT000377.1:intron
GATCAACTGTTCAAAAGAAC+TGG 0.398776 1:+5090532 MsaT000377.1:CDS
CTTGAGGCTTTAGCTGCTGC+AGG 0.425078 1:-5090814 None:intergenic
GCTAAAGCCTCAAGTATGTT+TGG 0.441559 1:+5090823 MsaT000377.1:CDS
ACACTAATTACCTTCTCTGT+TGG 0.465114 1:-5090889 None:intergenic
GGAAATTTGAGCCAGCTTGA+AGG 0.475377 1:+5091318 MsaT000377.1:CDS
GGAACAAGGGACAAATGTTC+TGG 0.477611 1:+5090847 MsaT000377.1:CDS
CAAGTATGTTTGGTGGAACA+AGG 0.495604 1:+5090833 MsaT000377.1:CDS
TCTCATGGTCACCTTCAAGC+TGG 0.497810 1:-5091329 None:intergenic
TTGAAACAACTCTTATGGTA+AGG 0.498185 1:-5091197 None:intergenic
AGAAAGAAATATAGATAATG+AGG 0.518674 1:+5090698 MsaT000377.1:CDS
CCTTGAGGGTTCCTTTGCAG+TGG 0.520760 1:-5090054 None:intergenic
GAGAGAGACATAGACCTTGA+GGG 0.525903 1:-5090068 None:intergenic
GACTAATAGTACACCCTCCA+TGG 0.536605 1:-5091226 None:intergenic
GCTTGATCAGTTGAATATGG+TGG 0.560624 1:-5091292 None:intergenic
AGGAACAACTCAGAAGTGTA+TGG 0.560932 1:+5089952 MsaT000377.1:CDS
AGTTGTTTCAAATGCTGCCA+TGG 0.564010 1:+5091209 MsaT000377.1:CDS
GACATCTGAAACAAGCTTTG+TGG 0.565667 1:-5090030 None:intergenic
TTCCAATTACATAGCACATG+AGG 0.574672 1:+5091250 MsaT000377.1:CDS
TTCAGATGTCACCACTGCAA+AGG 0.582786 1:+5090043 MsaT000377.1:CDS
AAAGCCTCAAGTATGTTTGG+TGG 0.587944 1:+5090826 MsaT000377.1:CDS
GTTTCAAATGCTGCCATGGA+GGG 0.597804 1:+5091213 MsaT000377.1:CDS
TTGAACAGTTGATCAAAGTG+TGG 0.606620 1:-5090523 None:intergenic
TGTTCCACCAAACATACTTG+AGG 0.606764 1:-5090830 None:intergenic
AACAGTGTATCCAACAGAGA+AGG 0.613878 1:+5090879 MsaT000377.1:CDS
TCCAATTACATAGCACATGA+GGG 0.619052 1:+5091251 MsaT000377.1:CDS
TCATGTGCTATGTAATTGGA+AGG 0.623562 1:-5091248 None:intergenic
AAGTATGTTTGGTGGAACAA+GGG 0.641097 1:+5090834 MsaT000377.1:CDS
CCACTGCAAAGGAACCCTCA+AGG 0.664342 1:+5090054 MsaT000377.1:CDS
ATATTCAACTGATCAAGCAA+AGG 0.664962 1:+5091297 MsaT000377.1:CDS
TGTTTCAAATGCTGCCATGG+AGG 0.668776 1:+5091212 MsaT000377.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr1 gene 5089941 5091337 5089941 ID=MsaG000377
Chr1 mRNA 5089941 5091337 5089941 ID=MsaT000377.1;Parent=MsaG000377
Chr1 exon 5089941 5090075 5089941 ID=MsaT000377.1.exon1;Parent=MsaT000377.1
Chr1 CDS 5089941 5090075 5089941 ID=cds.MsaT000377.1;Parent=MsaT000377.1
Chr1 exon 5090481 5090577 5090481 ID=MsaT000377.1.exon2;Parent=MsaT000377.1
Chr1 CDS 5090481 5090577 5090481 ID=cds.MsaT000377.1;Parent=MsaT000377.1
Chr1 exon 5090676 5090719 5090676 ID=MsaT000377.1.exon3;Parent=MsaT000377.1
Chr1 CDS 5090676 5090719 5090676 ID=cds.MsaT000377.1;Parent=MsaT000377.1
Chr1 exon 5090811 5090900 5090811 ID=MsaT000377.1.exon4;Parent=MsaT000377.1
Chr1 CDS 5090811 5090900 5090811 ID=cds.MsaT000377.1;Parent=MsaT000377.1
Chr1 exon 5091179 5091337 5091179 ID=MsaT000377.1.exon5;Parent=MsaT000377.1
Chr1 CDS 5091179 5091337 5091179 ID=cds.MsaT000377.1;Parent=MsaT000377.1
Gene Sequence

>MsaT000377.1

ATGGCATTTGCAGGAACAACTCAGAAGTGTATGGCTTGTAGCAAAACAGTTTATCTTGTTGATAAGTTAACTGCTGATAATAGAATTTTCCACAAAGCTTGTTTCAGATGTCACCACTGCAAAGGAACCCTCAAGCTAAGCAACTACAATTCTTTTGAGGGAGTTCTTTACTGCAGACCACACTTTGATCAACTGTTCAAAAGAACTGGTAGCCTTGAAAAAAGCTTTGAAGGGACACCAAAAATTGTCAAACCAGAAAGAAATATAGATAATGAGAAACCTGCAGCAGCTAAAGCCTCAAGTATGTTTGGTGGAACAAGGGACAAATGTTCTGGTTGTCAGAAAACAGTGTATCCAACAGAGAAGGTTACAGTGAATGGAACTCCTTACCATAAGAGTTGTTTCAAATGCTGCCATGGAGGGTGTACTATTAGTCCTTCCAATTACATAGCACATGAGGGAAAACTCTACTGCAAACACCACCATATTCAACTGATCAAGCAAAGGAAATTTGAGCCAGCTTGA

Protein sequence

>MsaT000377.1

MAFAGTTQKCMACSKTVYLVDKLTADNRIFHKACFRCHHCKGTLKLSNYNSFEGVLYCRPHFDQLFKRTGSLEKSFEGTPKIVKPERNIDNEKPAAAKASSMFGGTRDKCSGCQKTVYPTEKVTVNGTPYHKSCFKCCHGGCTISPSNYIAHEGKLYCKHHHIQLIKQRKFEPA*