AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG000472


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG000472 MtrunA17_Chr1g0151471 50.000 120 35 6 9 120 2 104 1.58e-20 80.5
MsaG000472 MtrunA17_Chr1g0151461 39.806 103 45 3 27 120 140 234 3.93e-12 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 24 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCAGTGTCAAGAGACTTAT+TGG 0.157774 1:-6239579 None:intergenic
AGTATTCGCCATTGCCCTTT+TGG 0.253039 1:-6239214 None:intergenic
TTCTTCTTTGTCGCTGGAAC+TGG 0.259226 1:-6239103 None:intergenic
AAGTCTCTTGACACTGAAAA+AGG 0.334844 1:+6239584 MsaT000472.1:CDS
ATACGAACACTACTCCTCTT+TGG 0.387924 1:+6239302 MsaT000472.1:CDS
GAATACTATATCCAAGGAAT+TGG 0.396437 1:+6239230 MsaT000472.1:CDS
TTGCCTCTTCCCTTCTTCGT+CGG 0.477939 1:-6239553 None:intergenic
ACCGAATACGAATACCCAAA+AGG 0.493067 1:+6239199 MsaT000472.1:CDS
GGAGGGCTGAACCAATTCCT+TGG 0.517307 1:-6239241 None:intergenic
TCTTTGGAGGAGTGATCCTA+CGG 0.527332 1:+6239318 MsaT000472.1:CDS
CCGAATACGAATACCCAAAA+GGG 0.528839 1:+6239200 MsaT000472.1:CDS
ATGTGGACTCCGACGAAGAA+GGG 0.540307 1:+6239544 MsaT000472.1:CDS
CGAACACTACTCCTCTTTGG+AGG 0.558044 1:+6239305 MsaT000472.1:CDS
ATAGTTAACAAGAAATATGT+TGG 0.559012 1:+6239502 MsaT000472.1:intron
GAGTGATCCTACGGGTTCCA+AGG 0.561497 1:+6239327 MsaT000472.1:CDS
CATGTGGACTCCGACGAAGA+AGG 0.573346 1:+6239543 MsaT000472.1:CDS
ACTCCGACGAAGAAGGGAAG+AGG 0.589069 1:+6239550 MsaT000472.1:CDS
CAGTCGTTGTGCAATGCATG+TGG 0.610062 1:+6239527 MsaT000472.1:CDS
ACGAATACCCAAAAGGGCAA+TGG 0.610371 1:+6239206 MsaT000472.1:CDS
CTTTGGAGGAGTGATCCTAC+GGG 0.615002 1:+6239319 MsaT000472.1:CDS
AATGGCGAATACTATATCCA+AGG 0.653439 1:+6239224 MsaT000472.1:CDS
ATCGCAATCGAAACAAACCC+TGG 0.664892 1:-6239281 None:intergenic
GTAGGATCACTCCTCCAAAG+AGG 0.676661 1:-6239316 None:intergenic
GTAATTACCTTGGAACCCGT+AGG 0.686052 1:-6239334 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr1 gene 6239082 6239655 6239082 ID=MsaG000472
Chr1 mRNA 6239082 6239655 6239082 ID=MsaT000472.1;Parent=MsaG000472
Chr1 exon 6239082 6239348 6239082 ID=MsaT000472.1.exon1;Parent=MsaT000472.1
Chr1 CDS 6239082 6239348 6239082 ID=cds.MsaT000472.1;Parent=MsaT000472.1
Chr1 exon 6239506 6239655 6239506 ID=MsaT000472.1.exon2;Parent=MsaT000472.1
Chr1 CDS 6239506 6239655 6239506 ID=cds.MsaT000472.1;Parent=MsaT000472.1
Gene Sequence

>MsaT000472.1

ATGAGAGTAAGTAACGATGATCCAGTTCCAGCGACAAAGAAGAAAAAAACTGCTGCTAGTGCTGCCAAAAAGAAGAAATCAGTTGCTAGTGCTTCCAAAAAGAAAATAAAAACTGCTACCGAATACGAATACCCAAAAGGGCAATGGCGAATACTATATCCAAGGAATTGGTTCAGCCCTCCTCCTAGTAAAAACACAACCAGGGTTTGTTTCGATTGCGATACGAACACTACTCCTCTTTGGAGGAGTGATCCTACGGGTTCCAAGTTAACAAGAAATATGTTGGTGCAGTCGTTGTGCAATGCATGTGGACTCCGACGAAGAAGGGAAGAGGCAAAAGCCAATAAGTCTCTTGACACTGAAAAAGGAAAGGGAAACGACGAAAGCTCATCATCTGAGAATGTGAAGAAGTTTTAA

Protein sequence

>MsaT000472.1

MRVSNDDPVPATKKKKTAASAAKKKKSVASASKKKIKTATEYEYPKGQWRILYPRNWFSPPPSKNTTRVCFDCDTNTTPLWRSDPTGSKLTRNMLVQSLCNACGLRRRREEAKANKSLDTEKGKGNDESSSSENVKKF*