AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG000479


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG000479 MtrunA17_Chr1g0151461 70.000 50 12 1 24 70 185 234 8.12e-20 80.9
MsaG000479 MtrunA17_Chr7g0271861 42.857 63 36 0 22 84 18 80 1.10e-15 66.6
MsaG000479 MtrunA17_Chr1g0151471 52.459 61 26 1 10 70 47 104 1.04e-14 63.5
MsaG000479 MtrunA17_Chr4g0071301 78.788 33 7 0 23 55 189 221 2.25e-14 66.2
MsaG000479 MtrunA17_Chr4g0068031 54.717 53 19 2 26 78 30 77 4.53e-14 63.2
MsaG000479 MtrunA17_Chr2g0299081 78.125 32 7 0 24 55 167 198 6.16e-14 65.1
MsaG000479 MtrunA17_Chr5g0404131 47.368 57 30 0 4 60 170 226 1.05e-12 61.6
MsaG000479 MtrunA17_Chr8g0373231 62.857 35 13 0 26 60 125 159 1.22e-11 58.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG000479 AT3G16870.1 53.226 62 28 1 24 85 40 100 4.08e-16 69.7
MsaG000479 AT5G56860.1 55.738 61 24 1 2 59 203 263 6.16e-16 71.2
MsaG000479 AT5G26930.1 48.276 58 21 1 24 72 24 81 8.27e-15 64.7
MsaG000479 AT5G49300.1 51.852 54 21 1 12 60 17 70 1.44e-14 64.3
MsaG000479 AT4G26150.1 73.529 34 9 0 24 57 197 230 1.68e-14 67.0
MsaG000479 AT2G18380.1 48.276 58 25 1 7 64 78 130 1.43e-13 63.2
MsaG000479 AT4G16141.1 62.222 45 16 1 24 68 35 78 1.45e-13 63.2
MsaG000479 AT4G36620.1 50.000 50 25 0 26 75 75 124 6.19e-13 61.6
MsaG000479 AT3G06740.1 66.667 33 11 0 28 60 43 75 3.20e-12 58.5
MsaG000479 AT3G50870.1 65.714 35 12 0 26 60 152 186 5.16e-12 60.1

Find 24 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATTCCTCCAAAGGGGAGTC+TGG 0.241417 1:-6277298 None:intergenic
GTGTTAAATGCTCTAGCCTT+TGG 0.380174 1:-6277227 None:intergenic
GGCAAAAGCTGCTAAAGGTT+TGG 0.402995 1:+6278250 MsaT000479.1:CDS
CCTTTGGAGGAATGGACCTA+TGG 0.408130 1:+6277307 MsaT000479.1:CDS
CAGACTCCCCTTTGGAGGAA+TGG 0.484698 1:+6277299 MsaT000479.1:CDS
AAACAACCCAGACTCCCCTT+TGG 0.489759 1:+6277291 MsaT000479.1:CDS
TGCTCTAGCCTTTGGATATG+TGG 0.489809 1:-6277219 None:intergenic
CAACCCAGACTCCCCTTTGG+AGG 0.510000 1:+6277294 MsaT000479.1:CDS
ATTCCTCCAAAGGGGAGTCT+GGG 0.513653 1:-6277297 None:intergenic
CTTTGGAGGAATGGACCTAT+GGG 0.526288 1:+6277308 MsaT000479.1:CDS
CATAGGTCCATTCCTCCAAA+GGG 0.526859 1:-6277306 None:intergenic
ACTCTAGTAGTTGTAGCAGC+TGG 0.535951 1:-6277254 None:intergenic
GAATGGACCTATGGGTCCTA+AGG 0.540865 1:+6277316 MsaT000479.1:CDS
AGTAGTTGTAGCAGCTGGAA+TGG 0.562471 1:-6277249 None:intergenic
CCATAGGTCCATTCCTCCAA+AGG 0.569794 1:-6277307 None:intergenic
CCTAAAACAGAGAAGGAAAG+TGG 0.579793 1:+6278229 MsaT000479.1:CDS
CTAATTACCTTAGGACCCAT+AGG 0.580046 1:-6277323 None:intergenic
AAAGTGGCAAAAGCTGCTAA+AGG 0.591042 1:+6278245 MsaT000479.1:CDS
CGATGAAAGCTCATCATCTG+AGG 0.597007 1:+6278295 MsaT000479.1:CDS
CATGTGGCCTAAAACAGAGA+AGG 0.633100 1:+6278222 MsaT000479.1:CDS
CAGTCGTTGTGCAACGCATG+TGG 0.634101 1:+6278206 MsaT000479.1:intron
TGCTACCACCACATATCCAA+AGG 0.634178 1:+6277211 MsaT000479.1:CDS
TCTAGCCTTTGGATATGTGG+TGG 0.675688 1:-6277216 None:intergenic
ATAGGTCCATTCCTCCAAAG+GGG 0.737737 1:-6277305 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr1 gene 6277197 6278334 6277197 ID=MsaG000479
Chr1 mRNA 6277197 6278334 6277197 ID=MsaT000479.1;Parent=MsaG000479
Chr1 exon 6277197 6277337 6277197 ID=MsaT000479.1.exon1;Parent=MsaT000479.1
Chr1 CDS 6277197 6277337 6277197 ID=cds.MsaT000479.1;Parent=MsaT000479.1
Chr1 exon 6278209 6278334 6278209 ID=MsaT000479.1.exon2;Parent=MsaT000479.1
Chr1 CDS 6278209 6278334 6278209 ID=cds.MsaT000479.1;Parent=MsaT000479.1
Gene Sequence

>MsaT000479.1

ATGAAGAAAACAACTGCTACCACCACATATCCAAAGGCTAGAGCATTTAACACCATTCCAGCTGCTACAACTACTAGAGTTTGTTCAGATTGTCAAACAACCCAGACTCCCCTTTGGAGGAATGGACCTATGGGTCCTAAGTCGTTGTGCAACGCATGTGGCCTAAAACAGAGAAGGAAAGTGGCAAAAGCTGCTAAAGGTTTGGACACTAAAAAAGAAAAGGGCAACGATGAAAGCTCATCATCTGAGGATGTGAAGAAGCTTTAA

Protein sequence

>MsaT000479.1

MKKTTATTTYPKARAFNTIPAATTTRVCSDCQTTQTPLWRNGPMGPKSLCNACGLKQRRKVAKAAKGLDTKKEKGNDESSSSEDVKKL*