AlfalfaGEDB | MsaG000527

M. sativa ssp. caerulea / MsaG000527

KEGG: -
GO: -
PFAM: PF02362.24

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG000527	PNY12437.1	73.723	274	72	0	1	274	1	274	3.03e-144	416

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG000527	sp\|Q8L3W1\|VRN1_ARATH	30.000	340	147	7	21	273	6	341	1.00e-39	144

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG000527	tr\|A0A2K3PAY3\|A0A2K3PAY3_TRIPR	73.723	274	72	0	1	274	1	274	1.45e-144	416

TFs/TRs:

Gene ID	Type	Classification
MsaG000527	TF	B3

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsaG000527	MsaG007452	0.809211	3.611064e-50	6.050120e-48
MsaG000527	MsaG013798	0.802332	9.907162e-49	1.412245e-46
MsaG000527	MsaG014133	0.829927	7.031576e-55	2.030789e-52
MsaG000527	MsaG014210	0.801764	1.294916e-48	1.822026e-46
MsaG000527	MsaG021219	0.810156	2.266874e-50	3.885919e-48
MsaG000527	MsaG040914	0.812072	8.752006e-51	1.572280e-48
MsaG000527	MsaG044869	0.803857	4.808945e-49	7.099883e-47
MsaG000527	MsaG045269	0.816579	8.929472e-52	1.796818e-49
MsaG000527	MsaG008315	0.807947	6.703657e-50	1.089548e-47
MsaG000527	MsaG033547	0.804804	3.060188e-49	4.618372e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG000527	MtrunA17_Chr5g0429931	68.852	244	76	0	31	274	5	248	8.45e-121	345
MsaG000527	MtrunA17_Chr3g0077451	67.220	241	68	2	35	274	10	240	4.88e-113	325
MsaG000527	MtrunA17_Chr3g0077411	63.019	265	90	2	18	274	259	523	2.93e-109	325
MsaG000527	MtrunA17_Chr3g0077411	52.256	266	100	3	1	262	1	243	1.33e-81	254
MsaG000527	MtrunA17_Chr3g0077421	66.667	210	58	2	65	272	1	200	1.61e-96	282
MsaG000527	MtrunA17_Chr3g0077441	66.667	210	58	2	65	272	1	200	1.61e-96	282
MsaG000527	MtrunA17_Chr3g0077431	64.455	211	64	2	65	274	1	201	2.58e-92	271
MsaG000527	MtrunA17_Chr4g0009491	63.158	228	82	1	3	228	205	432	2.32e-90	274
MsaG000527	MtrunA17_Chr4g0009491	52.113	213	80	1	35	247	11	201	6.58e-63	204
MsaG000527	MtrunA17_Chr3g0077361	56.554	267	92	3	1	265	20	264	2.35e-89	274
MsaG000527	MtrunA17_Chr3g0077361	53.650	274	90	2	3	274	269	507	1.35e-88	272
MsaG000527	MtrunA17_Chr3g0077381	59.211	228	84	2	3	228	227	447	2.70e-81	251
MsaG000527	MtrunA17_Chr3g0077381	48.319	238	77	3	30	265	29	222	1.20e-59	196
MsaG000527	MtrunA17_Chr3g0077461	51.793	251	102	5	18	266	234	467	9.16e-77	240
MsaG000527	MtrunA17_Chr3g0077461	51.055	237	85	3	36	271	22	228	8.23e-70	223
MsaG000527	MtrunA17_Chr1g0162441	45.455	99	54	0	21	119	7	105	1.07e-28	113
MsaG000527	MtrunA17_Chr1g0162441	37.190	121	64	4	158	269	276	393	3.20e-14	72.0
MsaG000527	MtrunA17_Chr3g0131891	26.644	289	175	6	20	271	18	306	5.35e-26	104
MsaG000527	MtrunA17_Chr3g0103181	28.986	276	161	8	20	267	11	279	1.42e-24	99.8
MsaG000527	MtrunA17_Chr4g0033961	41.176	102	60	0	18	119	3	104	1.90e-24	101
MsaG000527	MtrunA17_Chr4g0033961	35.115	131	76	3	147	271	264	391	9.38e-15	73.6
MsaG000527	MtrunA17_Chr5g0421921	42.857	112	63	1	161	272	2	112	2.49e-23	91.7
MsaG000527	MtrunA17_Chr5g0421731	42.857	112	63	1	161	272	2	112	2.49e-23	91.7
MsaG000527	MtrunA17_Chr1g0162411	43.820	89	50	0	21	109	7	95	5.03e-23	97.8
MsaG000527	MtrunA17_Chr3g0103171	28.517	263	155	5	36	267	5	265	6.91e-23	94.7
MsaG000527	MtrunA17_Chr7g0233561	26.421	299	173	7	20	271	17	315	2.10e-22	94.7
MsaG000527	MtrunA17_Chr8g0390841	25.497	302	164	8	21	269	39	332	2.83e-22	95.9
MsaG000527	MtrunA17_Chr5g0421851	42.105	114	65	1	159	272	75	187	5.23e-22	90.5
MsaG000527	MtrunA17_Chr1g0163071	27.891	294	144	9	37	269	2	288	2.18e-21	91.7
MsaG000527	MtrunA17_Chr1g0208771	26.027	292	146	8	36	269	12	291	5.80e-20	89.0
MsaG000527	MtrunA17_Chr7g0233521	24.542	273	160	7	37	271	2	266	8.39e-20	86.7
MsaG000527	MtrunA17_Chr7g0233571	27.215	158	115	0	12	169	1	158	9.48e-18	79.7
MsaG000527	MtrunA17_Chr5g0419201	30.827	133	85	1	21	153	16	141	1.34e-17	78.6
MsaG000527	MtrunA17_Chr3g0103191	29.439	214	123	5	21	209	12	222	1.88e-17	79.7
MsaG000527	MtrunA17_Chr3g0103151	39.000	100	59	1	20	119	11	108	7.06e-17	75.9
MsaG000527	MtrunA17_Chr1g0162421	44.828	58	32	0	35	92	7	64	7.54e-17	73.2
MsaG000527	MtrunA17_Chr1g0154161	25.623	281	173	8	20	267	29	306	1.68e-16	78.2
MsaG000527	MtrunA17_Chr1g0163221	23.826	298	178	9	13	269	3	292	3.68e-15	74.3
MsaG000527	MtrunA17_Chr1g0154131	26.740	273	157	8	34	264	195	466	3.80e-15	75.1
MsaG000527	MtrunA17_Chr4g0067431	23.779	307	170	8	20	269	14	313	1.06e-14	72.8
MsaG000527	MtrunA17_Chr7g0233531	22.143	280	158	5	37	271	2	266	2.18e-14	71.6
MsaG000527	MtrunA17_Chr6g0467351	32.374	139	85	3	10	142	4	139	1.40e-13	68.6
MsaG000527	MtrunA17_Chr7g0233541	30.973	113	74	1	37	149	2	110	3.30e-13	68.2
MsaG000527	MtrunA17_Chr3g0103201	25.463	216	133	4	80	267	5	220	4.61e-12	63.9
MsaG000527	MtrunA17_Chr1g0163061	30.702	114	78	1	10	122	4	117	6.35e-12	64.3
MsaG000527	MtrunA17_Chr1g0171611	23.019	265	181	7	19	266	7	265	1.05e-11	63.9

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG000527	AT3G18990.1	30.000	340	147	7	21	273	6	341	1.02e-40	144
MsaG000527	AT3G18990.2	30.205	341	147	7	21	274	6	342	1.18e-40	144
MsaG000527	AT1G49475.1	37.302	126	74	2	4	129	21	141	7.19e-19	82.4
MsaG000527	AT4G01580.1	36.036	111	71	0	14	124	23	133	1.26e-18	82.0
MsaG000527	AT3G18960.2	36.036	111	71	0	14	124	23	133	1.51e-18	81.6
MsaG000527	AT3G18960.1	36.036	111	71	0	14	124	23	133	1.52e-18	82.0
MsaG000527	AT1G49480.3	33.083	133	79	4	147	273	98	226	5.54e-14	70.1
MsaG000527	AT1G49480.1	33.083	133	79	4	147	273	98	226	5.54e-14	70.1
MsaG000527	AT1G49480.2	33.083	133	79	4	147	273	98	226	5.54e-14	70.1
MsaG000527	AT3G18960.3	35.955	89	57	0	36	124	8	96	4.45e-13	66.2
MsaG000527	AT4G31690.1	23.108	251	163	9	12	257	7	232	3.81e-11	62.4

Find 53 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTGTTCCGGATGGTTGTATT+TGG	0.168306	1:+6853151	MsaT000527.1:CDS
TTGTTGGCCCTTTGAAATTT+AGG	0.191348	1:-6853779	None:intergenic
ATTGCTGTCCATGAGAATTT+TGG	0.193809	1:-6853694	None:intergenic
AATGAGTTTATCGCAAGATT+TGG	0.267691	1:+6853099	MsaT000527.1:CDS
AAGTGAGAGCCGTGGGAAAA+AGG	0.303768	1:+6853419	MsaT000527.1:CDS
TGATAGGAACATTTGGCTTC+AGG	0.319381	1:-6853667	None:intergenic
TTCCTTCATAATTGAAAGAA+AGG	0.357561	1:-6853266	None:intergenic
AATCTTGCGATAAACTCATT+TGG	0.373859	1:-6853096	None:intergenic
GGATGGTTGTATTTGGGAAA+TGG	0.374541	1:+6853158	MsaT000527.1:CDS
TGTTGCTACAATTACTGTTC+CGG	0.380706	1:+6853137	MsaT000527.1:CDS
TGTTCCGGATGGTTGTATTT+GGG	0.395192	1:+6853152	MsaT000527.1:CDS
ATGAATGGGTGAGGGAAGTA+TGG	0.397613	1:-6852088	None:intergenic
TGGAGTTTGATAGGAACATT+TGG	0.399156	1:-6853674	None:intergenic
GAATACTATTCAATAGGATA+TGG	0.402689	1:+6853237	MsaT000527.1:CDS
TACCTTTCTTTCAATTATGA+AGG	0.405245	1:+6853264	MsaT000527.1:CDS
GTTTATTGGGTTGAATTCAT+TGG	0.425223	1:-6853487	None:intergenic
AAAGAAGCCGATTGTGCTAA+AGG	0.434126	1:+6853841	MsaT000527.1:CDS
CATTTGCATTGATAATGATT+CGG	0.444491	1:-6853744	None:intergenic
ATCTCCACGCGACAGATTGT+TGG	0.449174	1:-6853795	None:intergenic
GAGAAGACCTGTCCGGTCAT+TGG	0.449255	1:+6852032	MsaT000527.1:CDS
TTCATAGAATACTATTCAAT+AGG	0.452230	1:+6853231	MsaT000527.1:CDS
ATTGTGACCTTTAGCACAAT+CGG	0.459095	1:-6853848	None:intergenic
CGAATCATTATCAATGCAAA+TGG	0.470308	1:+6853745	MsaT000527.1:CDS
TACTATTTGAGTGGCATGAA+TGG	0.470527	1:-6852103	None:intergenic
AAATTCTCATGGACAGCAAT+GGG	0.472438	1:+6853697	MsaT000527.1:CDS
AAACAAAGTATCCTAGTCCT+AGG	0.485163	1:+6853376	MsaT000527.1:CDS
ACCTGTCCGGTCATTGGCGC+CGG	0.487744	1:+6852038	MsaT000527.1:CDS
ATCAAACTCCAAAATTCTCA+TGG	0.489771	1:+6853686	MsaT000527.1:CDS
CGTTCTCCGGCGCCAATGAC+CGG	0.495879	1:-6852044	None:intergenic
AGGATTTCCTAAATTTCAAA+GGG	0.499352	1:+6853772	MsaT000527.1:CDS
AGTATCCTAGTCCTAGGAAA+AGG	0.513608	1:+6853382	MsaT000527.1:CDS
TTCAAAACTCTAAGTACTAG+TGG	0.514800	1:+6853345	MsaT000527.1:CDS
AAAATTCTCATGGACAGCAA+TGG	0.519153	1:+6853696	MsaT000527.1:CDS
GAGGATTTCCTAAATTTCAA+AGG	0.521737	1:+6853771	MsaT000527.1:CDS
ATGACCGGACAGGTCTTCTC+CGG	0.525069	1:-6852029	None:intergenic
ACTATTTGAGTGGCATGAAT+GGG	0.552409	1:-6852102	None:intergenic
ATTTCCCAAATACAACCATC+CGG	0.559087	1:-6853156	None:intergenic
TCCGGCGCCAATGACCGGAC+AGG	0.576662	1:-6852039	None:intergenic
AATCTGCAATGAAGCCTCGC+CGG	0.581440	1:+6852010	None:intergenic
ACTATTCAATAGGATATGGT+TGG	0.585720	1:+6853241	MsaT000527.1:CDS
ACCGAAACTTACTATTTGAG+TGG	0.604521	1:-6852112	None:intergenic
CTCGCCGGAGAAGACCTGTC+CGG	0.607153	1:+6852025	MsaT000527.1:CDS
TTGAGTGGCATGAATGGGTG+AGG	0.609441	1:-6852097	None:intergenic
TTAAAACAAGTGAGAGCCGT+GGG	0.615648	1:+6853412	MsaT000527.1:CDS
TAGTCCTAGGAAAAGGTCCA+AGG	0.626880	1:+6853389	MsaT000527.1:CDS
GCTACAATTACTGTTCCGGA+TGG	0.637015	1:+6853141	MsaT000527.1:CDS
AGGGCCAACAATCTGTCGCG+TGG	0.637859	1:+6853791	MsaT000527.1:CDS
AATTGCTAAGGCAAATCGAA+CGG	0.646365	1:+6853527	MsaT000527.1:CDS
GTTAAAACAAGTGAGAGCCG+TGG	0.656728	1:+6853411	MsaT000527.1:CDS
TGAGTGGCATGAATGGGTGA+GGG	0.662387	1:-6852096	None:intergenic
GAAATGGAACTGAAGAAATG+CGG	0.682734	1:+6853174	MsaT000527.1:CDS
TTATCAATGCAAATGGCACG+AGG	0.695216	1:+6853752	MsaT000527.1:CDS
CGGACAGGTCTTCTCCGGCG+AGG	0.701745	1:-6852024	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content

Chromosome	Type	Strat	End	Strand	Name
Chr1	gene	6852018	6853898	6852018	ID=MsaG000527
Chr1	mRNA	6852018	6853898	6852018	ID=MsaT000527.1;Parent=MsaG000527
Chr1	exon	6852018	6852122	6852018	ID=MsaT000527.1.exon1;Parent=MsaT000527.1
Chr1	CDS	6852018	6852122	6852018	ID=cds.MsaT000527.1;Parent=MsaT000527.1
Chr1	exon	6853090	6853548	6853090	ID=MsaT000527.1.exon2;Parent=MsaT000527.1
Chr1	CDS	6853090	6853548	6853090	ID=cds.MsaT000527.1;Parent=MsaT000527.1
Chr1	exon	6853638	6853898	6853638	ID=MsaT000527.1.exon3;Parent=MsaT000527.1
Chr1	CDS	6853638	6853898	6853638	ID=cds.MsaT000527.1;Parent=MsaT000527.1

Gene Sequence

>MsaT000527.1

ATGAAGCCTCGCCGGAGAAGACCTGTCCGGTCATTGGCGCCGGAGAACGTATCAAAACATTTTATGAAAGCCATACTTCCCTCACCCATTCATGCCACTCAAATAAGAATTCCAAATGAGTTTATCGCAAGATTTGGAAATGAACTCAAGAATGTTGCTACAATTACTGTTCCGGATGGTTGTATTTGGGAAATGGAACTGAAGAAATGCGGCAATGACGTTTTCTTTTGTAACAAATGGCAAGAGTTCATAGAATACTATTCAATAGGATATGGTTGGTACCTTTCTTTCAATTATGAAGGAAATTCAAAGTTTAGTGTTACTATAATTGATGTAACTTCTGTTGAGATTTGCTATCCGTTCAAAACTCTAAGTACTAGTGGAGAACCAAAAACAAAGTATCCTAGTCCTAGGAAAAGGTCCAAGGTTAAAACAAGTGAGAGCCGTGGGAAAAAGGACAAAAACATGTCTACGCGTGCTTCTAAAAAAGCAGAGAAGGCTGCCAATGAATTCAACCCAATAAACCCTTTTTTTCGTTCAAAAATTGCTAAGGCAAATCGAACGAGTGTTACTTCTAAATTTGCTCAAAAGTACCTGAAGCCAAATGTTCCTATCAAACTCCAAAATTCTCATGGACAGCAATGGGAAGTTTCTTGTGTATTTTGTAATGCCGAATCATTATCAATGCAAATGGCACGAGGATTTCCTAAATTTCAAAGGGCCAACAATCTGTCGCGTGGAGATTATTGTGCGTTTGAGCTGATAAAAAAGAAGCCGATTGTGCTAAAGGTCACAATGTTTCGTGTAGTTGATTATCTTGATTAA

Protein sequence

>MsaT000527.1

MKPRRRRPVRSLAPENVSKHFMKAILPSPIHATQIRIPNEFIARFGNELKNVATITVPDGCIWEMELKKCGNDVFFCNKWQEFIEYYSIGYGWYLSFNYEGNSKFSVTIIDVTSVEICYPFKTLSTSGEPKTKYPSPRKRSKVKTSESRGKKDKNMSTRASKKAEKAANEFNPINPFFRSKIAKANRTSVTSKFAQKYLKPNVPIKLQNSHGQQWEVSCVFCNAESLSMQMARGFPKFQRANNLSRGDYCAFELIKKKPIVLKVTMFRVVDYLD*