AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG000533


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG000533 MtrunA17_Chr1g0150821 99.242 264 1 1 1 264 1 263 0.0 543
MsaG000533 MtrunA17_Chr8g0343441 78.378 259 45 3 8 264 7 256 1.90e-130 369
MsaG000533 MtrunA17_Chr2g0300241 74.809 262 49 3 5 264 7 253 3.98e-125 356
MsaG000533 MtrunA17_Chr4g0076771 70.588 255 64 3 10 263 10 254 2.95e-113 326
MsaG000533 MtrunA17_Chr2g0329821 72.441 254 60 3 11 263 6 250 5.75e-111 320
MsaG000533 MtrunA17_Chr4g0006381 62.500 256 77 4 9 264 6 242 4.22e-94 277
MsaG000533 MtrunA17_Chr7g0242511 62.500 256 77 6 9 264 6 242 1.67e-92 273
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG000533 AT5G20510.1 71.591 264 71 2 1 264 1 260 6.07e-124 353
MsaG000533 AT3G42790.1 72.348 264 57 3 1 263 1 249 1.08e-122 350
MsaG000533 AT2G02470.1 70.196 255 69 3 10 264 9 256 9.23e-111 320
MsaG000533 AT2G02470.2 67.578 256 65 3 10 264 9 247 2.32e-109 316
MsaG000533 AT5G26210.1 78.077 260 49 1 4 263 3 254 2.14e-108 314
MsaG000533 AT1G14510.1 67.063 252 71 2 10 261 9 248 6.38e-107 310
MsaG000533 AT5G20510.2 71.364 220 59 2 45 264 4 219 4.27e-98 286
MsaG000533 AT5G05610.2 61.961 255 76 5 9 263 7 240 1.38e-97 286
MsaG000533 AT5G05610.1 61.961 255 76 5 9 263 7 240 1.38e-97 286
MsaG000533 AT3G11200.1 61.977 263 82 3 1 263 1 245 1.32e-96 283
MsaG000533 AT5G20510.3 77.914 163 36 0 6 168 6 168 1.47e-82 246
MsaG000533 AT3G11200.2 62.100 219 65 3 45 263 32 232 3.31e-80 241
MsaG000533 AT2G02470.3 79.333 150 30 1 10 159 9 157 3.11e-72 222

Find 65 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGGAAAATCTTTGCCTTTA+TGG 0.094878 1:+6957131 MsaT000533.1:CDS
AGAAAAGGATTGGCTATCTT+TGG 0.280443 1:+6957256 MsaT000533.1:CDS
GCTATGCAGCTGACGAGTTC+TGG 0.291054 1:+6959018 MsaT000533.1:CDS
ACAATCTTTGAAGTTGTTAC+AGG 0.306235 1:+6958278 MsaT000533.1:CDS
CTTAGAGTTTGATTTGGATT+TGG 0.307586 1:-6958354 None:intergenic
TTTGAAGTTGTTACAGGGTC+TGG 0.380524 1:+6958284 MsaT000533.1:CDS
TTGATATGCTCAGCCCTTGC+CGG 0.382099 1:-6959095 None:intergenic
GCTTACCTTAGAGTTTGATT+TGG 0.384520 1:-6958360 None:intergenic
GTAACAACTTCAAAGATTGT+TGG 0.390377 1:-6958275 None:intergenic
CAAATCCAAATCAAACTCTA+AGG 0.411797 1:+6958355 MsaT000533.1:CDS
TTAATACCCAATGCTGGCTC+TGG 0.427895 1:-6957212 None:intergenic
TTGGATTTGACAAGGCTGAT+AGG 0.428891 1:+6957336 MsaT000533.1:CDS
GCTCGCTCGGAAATCCATAA+AGG 0.438097 1:-6957145 None:intergenic
GGTATTAACTTTGCTAGAGA+TGG 0.438852 1:+6957227 MsaT000533.1:CDS
TCAGGCGGCACTTCCTCAGC+TGG 0.444344 1:-6957185 None:intergenic
AATGCTGGCTCTGGAAGCTC+AGG 0.453734 1:-6957203 None:intergenic
ATCTGCATTGCCATTGCCAT+TGG 0.459684 1:-6958897 None:intergenic
GCTCTCTTGTTGCTGCAAGC+TGG 0.467608 1:-6959131 None:intergenic
TTCACCTCCCACTGCTCGCT+CGG 0.467752 1:-6957158 None:intergenic
TCGACAGTACGAGGGTTGTA+CGG 0.497527 1:-6955020 None:intergenic
GCTGCGACATATGTGAGAAA+TGG 0.501712 1:+6959045 MsaT000533.1:CDS
TGATGCTTGGTTGCTTTCTG+TGG 0.503077 1:+6957292 MsaT000533.1:CDS
TTCTACCAGCAGTGTGATCC+CGG 0.504757 1:+6955213 MsaT000533.1:CDS
ACAACAACCATGGACGTTGT+AGG 0.512436 1:+6954993 None:intergenic
ATGGAATGCAAGAAAAGGAT+TGG 0.512817 1:+6957246 MsaT000533.1:CDS
AAGTTGTTACAGGGTCTGGC+AGG 0.518216 1:+6958288 MsaT000533.1:CDS
AGAAGACCGACCGGTGGTGA+GGG 0.523124 1:-6955090 None:intergenic
TAGAGATGGAATGCAAGAAA+AGG 0.524287 1:+6957241 MsaT000533.1:CDS
GAGCTTCCAGAGCCAGCATT+GGG 0.524583 1:+6957206 MsaT000533.1:CDS
TTAATTAAAGCCCTCACCAC+CGG 0.530739 1:+6955080 MsaT000533.1:CDS
AAGACTTCTTCGACAGTACG+AGG 0.536014 1:-6955029 None:intergenic
TTGCAGTACACAGTGATGCT+TGG 0.538841 1:+6957279 MsaT000533.1:CDS
GAAGTCTTTCGAGATTTCAA+AGG 0.539441 1:+6955044 MsaT000533.1:CDS
ACAGGGTCTGGCAGGAAACA+AGG 0.548267 1:+6958296 MsaT000533.1:CDS
AGGTGTGGACGAGGAAGATG+AGG 0.549480 1:+6958956 MsaT000533.1:CDS
TGCTAGGTTTGGATTTGACA+AGG 0.554851 1:+6957328 MsaT000533.1:CDS
CAATCTTTGAAGTTGTTACA+GGG 0.559430 1:+6958279 MsaT000533.1:CDS
CGGAGCACCTACAACGTCCA+TGG 0.562602 1:-6955000 None:intergenic
GCAAAGTTAATACCCAATGC+TGG 0.563035 1:-6957218 None:intergenic
GCAGTCAAAGGATGAGGTTG+AGG 0.565823 1:+6958920 MsaT000533.1:CDS
ATATGTGAGAAATGGTTTCA+CGG 0.569510 1:+6959053 MsaT000533.1:CDS
GCAGATGCAGTCAAAGGATG+AGG 0.569670 1:+6958914 MsaT000533.1:CDS
TGAGCTTCCAGAGCCAGCAT+TGG 0.570153 1:+6957205 MsaT000533.1:CDS
CGTGAAGATTACTCCGGCAA+GGG 0.576974 1:+6959082 MsaT000533.1:CDS
TGGATTTCCGAGCGAGCAGT+GGG 0.579516 1:+6957151 MsaT000533.1:CDS
GGCAATGCAGATGCAGTCAA+AGG 0.579948 1:+6958908 MsaT000533.1:CDS
GCTGGCTCTGGAAGCTCAGG+CGG 0.581111 1:-6957200 None:intergenic
GCGTGAAGATTACTCCGGCA+AGG 0.589663 1:+6959081 MsaT000533.1:CDS
GAGAAGACCGACCGGTGGTG+AGG 0.592144 1:-6955091 None:intergenic
ATGGATTTCCGAGCGAGCAG+TGG 0.601477 1:+6957150 MsaT000533.1:CDS
TATTACCGGGATCACACTGC+TGG 0.607631 1:-6955218 None:intergenic
GAGACTTTGTGTGGGGCATG+TGG 0.613065 1:+6958990 MsaT000533.1:CDS
AACACGGAGAGACTTTGTGT+GGG 0.622939 1:+6958982 MsaT000533.1:CDS
GGATGAAGATGGTGTAGGTG+TGG 0.626967 1:+6958941 MsaT000533.1:CDS
AGATGGTGTAGGTGTGGACG+AGG 0.628142 1:+6958947 MsaT000533.1:CDS
GAACACGGAGAGACTTTGTG+TGG 0.630911 1:+6958981 MsaT000533.1:CDS
GGAGGTGAATCTGCCAGCTG+AGG 0.634327 1:+6957172 MsaT000533.1:CDS
AGACTTCTTCGACAGTACGA+GGG 0.643030 1:-6955028 None:intergenic
GATGAGGTTGAGGATGAAGA+TGG 0.646194 1:+6958930 MsaT000533.1:CDS
GAGGAAGATGAGGAAGAACA+CGG 0.666720 1:+6958966 MsaT000533.1:CDS
ATTTCCGAGCGAGCAGTGGG+AGG 0.670558 1:+6957154 MsaT000533.1:CDS
GTTGAGGATGAAGATGGTGT+AGG 0.671969 1:+6958936 MsaT000533.1:CDS
GTCTGGCAGGAAACAAGGAA+AGG 0.676618 1:+6958301 MsaT000533.1:CDS
ACACGGAGAGACTTTGTGTG+GGG 0.707279 1:+6958983 MsaT000533.1:CDS
AAAATGCGTGAAGATTACTC+CGG 0.732353 1:+6959076 MsaT000533.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr1 gene 6955002 6959163 6955002 ID=MsaG000533
Chr1 mRNA 6955002 6959163 6955002 ID=MsaT000533.1;Parent=MsaG000533
Chr1 exon 6955002 6955101 6955002 ID=MsaT000533.1.exon1;Parent=MsaT000533.1
Chr1 CDS 6955002 6955101 6955002 ID=cds.MsaT000533.1;Parent=MsaT000533.1
Chr1 exon 6955202 6955234 6955202 ID=MsaT000533.1.exon2;Parent=MsaT000533.1
Chr1 CDS 6955202 6955234 6955202 ID=cds.MsaT000533.1;Parent=MsaT000533.1
Chr1 exon 6957129 6957357 6957129 ID=MsaT000533.1.exon3;Parent=MsaT000533.1
Chr1 CDS 6957129 6957357 6957129 ID=cds.MsaT000533.1;Parent=MsaT000533.1
Chr1 exon 6958244 6958376 6958244 ID=MsaT000533.1.exon4;Parent=MsaT000533.1
Chr1 CDS 6958244 6958376 6958244 ID=cds.MsaT000533.1;Parent=MsaT000533.1
Chr1 exon 6958864 6959163 6958864 ID=MsaT000533.1.exon5;Parent=MsaT000533.1
Chr1 CDS 6958864 6959163 6958864 ID=cds.MsaT000533.1;Parent=MsaT000533.1
Gene Sequence

>MsaT000533.1

ATGGACGTTGTAGGTGCTCCGTACAACCCTCGTACTGTCGAAGAAGTCTTTCGAGATTTCAAAGGTCGCAGAGCTGCTTTAATTAAAGCCCTCACCACCGATGTCGATGTATTCTACCAGCAGTGTGATCCCGAGAAGGAAAATCTTTGCCTTTATGGATTTCCGAGCGAGCAGTGGGAGGTGAATCTGCCAGCTGAGGAAGTGCCGCCTGAGCTTCCAGAGCCAGCATTGGGTATTAACTTTGCTAGAGATGGAATGCAAGAAAAGGATTGGCTATCTTTGGTTGCAGTACACAGTGATGCTTGGTTGCTTTCTGTGGCTTTTTATTTCGGTGCTAGGTTTGGATTTGACAAGGCTGATAGGAAGCGACTGTTTAATATGATCAATGATCTTCCAACAATCTTTGAAGTTGTTACAGGGTCTGGCAGGAAACAAGGAAAGGAAAAGTCATCAGTTTCTAATAATAGCAGTACCAAATCCAAATCAAACTCTAAGCGAAGTTCTGAACCTCAGCCAAAGTTTTCAAAACCAATGGCAATGGCAATGCAGATGCAGTCAAAGGATGAGGTTGAGGATGAAGATGGTGTAGGTGTGGACGAGGAAGATGAGGAAGAACACGGAGAGACTTTGTGTGGGGCATGTGGAGACAGCTATGCAGCTGACGAGTTCTGGATTTGCTGCGACATATGTGAGAAATGGTTTCACGGAAAATGCGTGAAGATTACTCCGGCAAGGGCTGAGCATATCAAACAGTACAAATGTCCAGCTTGCAGCAACAAGAGAGCTCGCCCTTGA

Protein sequence

>MsaT000533.1

MDVVGAPYNPRTVEEVFRDFKGRRAALIKALTTDVDVFYQQCDPEKENLCLYGFPSEQWEVNLPAEEVPPELPEPALGINFARDGMQEKDWLSLVAVHSDAWLLSVAFYFGARFGFDKADRKRLFNMINDLPTIFEVVTGSGRKQGKEKSSVSNNSSTKSKSNSKRSSEPQPKFSKPMAMAMQMQSKDEVEDEDGVGVDEEDEEEHGETLCGACGDSYAADEFWICCDICEKWFHGKCVKITPARAEHIKQYKCPACSNKRARP*