AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG000537


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG000537 MtrunA17_Chr1g0150801 97.521 121 3 0 1 121 1 121 1.39e-85 246
MsaG000537 MtrunA17_Chr8g0383251 41.176 102 50 3 25 120 29 126 2.22e-13 63.5
MsaG000537 MtrunA17_Chr3g0129321 37.984 129 66 4 5 121 11 137 3.92e-13 63.2
MsaG000537 MtrunA17_Chr7g0270191 63.415 41 15 0 81 121 124 164 3.48e-11 58.5
MsaG000537 MtrunA17_Chr1g0194481 50.943 53 26 0 69 121 125 177 5.47e-11 58.2
MsaG000537 MtrunA17_Chr4g0004911 64.103 39 13 1 84 121 317 355 9.78e-11 57.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG000537 AT5G26170.1 78.788 33 7 0 89 121 100 132 2.59e-14 65.9
MsaG000537 AT2G47260.1 61.905 42 16 0 80 121 152 193 2.09e-11 59.7
MsaG000537 AT5G07100.2 60.870 46 15 2 82 124 118 163 4.18e-11 58.2
MsaG000537 AT5G07100.4 60.870 46 15 2 82 124 118 163 4.18e-11 58.2
MsaG000537 AT5G07100.5 60.870 46 15 2 82 124 118 163 4.18e-11 58.2
MsaG000537 AT5G07100.3 60.870 46 15 2 82 124 118 163 4.18e-11 58.2
MsaG000537 AT3G01970.1 69.697 33 10 0 89 121 52 84 4.83e-11 57.0
MsaG000537 AT5G64810.1 33.913 115 64 4 18 120 14 128 6.03e-11 57.4
MsaG000537 AT5G07100.1 65.116 43 12 2 82 121 211 253 7.07e-11 58.2

Find 24 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAATATATATACCTTGGATT+AGG 0.247704 1:-7003283 None:intergenic
GATGATGAATTTGCTGTTCT+TGG 0.294438 1:-7002808 None:intergenic
TGGTTGCTAAAATCACTTTC+AGG 0.298136 1:-7002832 None:intergenic
AATATATATACCTTGGATTA+GGG 0.308746 1:-7003282 None:intergenic
ATATGAGTGGAAACGATAAT+TGG 0.349180 1:+7003152 MsaT000537.1:CDS
GGGTACAGATGGAGGAAGTA+TGG 0.355517 1:+7003238 MsaT000537.1:CDS
TAATTGGAAGAAGGAAATTA+AGG 0.361306 1:+7003168 MsaT000537.1:CDS
GAGGAAGTATGGAAAGAAAA+TGG 0.372428 1:+7003249 MsaT000537.1:CDS
TGAGTTTCGATGACAATGAA+TGG 0.486049 1:+7002884 MsaT000537.1:CDS
TTTCAAGCCAACGAAGTAGC+AGG 0.492097 1:+7002940 MsaT000537.1:CDS
AAGTAGTCAGAGAGTTCAAA+TGG 0.505113 1:-7002862 None:intergenic
GCTGCCACCTGCTACTTCGT+TGG 0.513271 1:-7002947 None:intergenic
GCAGGTGGCAGCTACATTGA+AGG 0.517291 1:+7002958 MsaT000537.1:CDS
AAAACTAAAGACTCAGTGGT+TGG 0.518786 1:-7002919 None:intergenic
AGAGTTCAAATGGCCACTGC+TGG 0.523946 1:-7002852 None:intergenic
CTGATTGAAATACTTGATGA+TGG 0.529008 1:+7003217 MsaT000537.1:CDS
TGGAAACGATAATTGGAAGA+AGG 0.531499 1:+7003159 MsaT000537.1:CDS
TACTTGATGATGGGTACAGA+TGG 0.541635 1:+7003227 MsaT000537.1:CDS
ACTAATAATATGAATATGAG+TGG 0.553406 1:+7003139 MsaT000537.1:CDS
CAGGTGGCAGCTACATTGAA+GGG 0.604012 1:+7002959 MsaT000537.1:CDS
TGATTGAAATACTTGATGAT+GGG 0.620660 1:+7003218 MsaT000537.1:CDS
TTGATGATGGGTACAGATGG+AGG 0.631832 1:+7003230 MsaT000537.1:CDS
TTAAGAACAGCCCTAATCCA+AGG 0.633554 1:+7003272 MsaT000537.1:CDS
CAAGCCAACGAAGTAGCAGG+TGG 0.667226 1:+7002943 MsaT000537.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr1 gene 7002793 7013206 7002793 ID=MsaG000537
Chr1 mRNA 7002793 7013206 7002793 ID=MsaT000537.1;Parent=MsaG000537
Chr1 exon 7002793 7002997 7002793 ID=MsaT000537.1.exon1;Parent=MsaT000537.1
Chr1 CDS 7002793 7002997 7002793 ID=cds.MsaT000537.1;Parent=MsaT000537.1
Chr1 exon 7003134 7003293 7003134 ID=MsaT000537.1.exon2;Parent=MsaT000537.1
Chr1 CDS 7003134 7003293 7003134 ID=cds.MsaT000537.1;Parent=MsaT000537.1
Chr1 exon 7013197 7013206 7013197 ID=MsaT000537.1.exon3;Parent=MsaT000537.1
Chr1 CDS 7013197 7013206 7013197 ID=cds.MsaT000537.1;Parent=MsaT000537.1
Gene Sequence

>MsaT000537.1

ATGACAGAGAAAAATCCAAGAACAGCAAATTCATCATCACCTGAAAGTGATTTTAGCAACCAGCAGTGGCCATTTGAACTCTCTGACTACTTGAGTTTCGATGACAATGAATGGCTACAAAACAATCCAACCACTGAGTCTTTAGTTTTTCAAGCCAACGAAGTAGCAGGTGGCAGCTACATTGAAGGGTCTTCTAGTTCAATCAGAGATACTAATAATATGAATATGAGTGGAAACGATAATTGGAAGAAGGAAATTAAGGAGAGAGTTGCATTCAAAACAAAGTCACTGATTGAAATACTTGATGATGGGTACAGATGGAGGAAGTATGGAAAGAAAATGGTTAAGAACAGCCCTAATCCAAGCTTGTTTTAA

Protein sequence

>MsaT000537.1

MTEKNPRTANSSSPESDFSNQQWPFELSDYLSFDDNEWLQNNPTTESLVFQANEVAGGSYIEGSSSSIRDTNNMNMSGNDNWKKEIKERVAFKTKSLIEILDDGYRWRKYGKKMVKNSPNPSLF*