AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG000546


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG000546 MtrunA17_Chr1g0150721 95.105 143 6 1 146 288 1 142 3.64e-97 281
MsaG000546 MtrunA17_Chr3g0136431 59.060 149 49 4 146 288 1 143 1.32e-53 171
MsaG000546 MtrunA17_Chr1g0202341 66.964 112 36 1 1 112 14 124 6.41e-44 158
MsaG000546 MtrunA17_Chr3g0077241 60.215 93 32 3 1 92 1 89 5.12e-32 115
MsaG000546 MtrunA17_Chr4g0052331 53.846 91 41 1 1 91 6 95 1.15e-27 102
MsaG000546 MtrunA17_Chr4g0008841 31.034 145 71 4 147 283 13 136 3.63e-17 76.3
MsaG000546 MtrunA17_Chr7g0240831 31.298 131 69 2 147 277 22 131 3.16e-15 71.2
MsaG000546 MtrunA17_Chr7g0240841 31.298 131 69 2 147 277 22 131 3.16e-15 71.2
MsaG000546 MtrunA17_Chr2g0329361 51.515 66 32 0 37 102 59 124 3.91e-15 73.6
MsaG000546 MtrunA17_Chr7g0240861 30.000 140 76 4 147 284 27 146 3.88e-14 68.6
MsaG000546 MtrunA17_Chr4g0032581 30.645 124 64 3 150 273 35 136 7.51e-14 67.8
MsaG000546 MtrunA17_Chr4g0032571 28.455 123 66 2 146 268 27 127 5.49e-13 65.1
MsaG000546 MtrunA17_Chr4g0032291 31.579 133 66 4 150 278 46 157 1.60e-12 64.3
MsaG000546 MtrunA17_Chr2g0329421 40.541 74 40 1 24 93 8 81 7.88e-12 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG000546 AT5G20650.1 55.102 147 61 3 146 288 1 146 3.62e-48 157
MsaG000546 AT2G26975.1 33.333 150 72 4 125 273 9 131 1.46e-20 86.3
MsaG000546 AT5G59040.1 33.333 144 69 3 130 273 19 135 5.57e-18 79.3
MsaG000546 AT3G06250.2 45.679 81 41 1 16 93 2 82 8.95e-14 72.0
MsaG000546 AT3G06250.1 45.679 81 41 1 16 93 2 82 8.95e-14 72.0
MsaG000546 AT3G06250.3 45.679 81 41 1 16 93 2 82 8.95e-14 72.0
MsaG000546 AT2G37925.1 30.769 130 72 2 144 273 27 138 2.09e-13 66.6
MsaG000546 AT5G59030.1 27.778 144 80 3 150 287 45 170 6.65e-13 65.9
MsaG000546 AT3G46900.1 27.465 142 77 4 152 287 35 156 1.96e-12 64.3
MsaG000546 AT5G18960.2 49.153 59 30 0 35 93 39 97 5.87e-12 66.2
MsaG000546 AT5G18960.1 49.153 59 30 0 35 93 39 97 5.87e-12 66.2

Find 62 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCAACATTCTACCAATATT+TGG 0.138215 1:-7082191 MsaT000546.1:CDS
AATGGTAGTTCGTTATTTAT+AGG 0.193209 1:+7084211 None:intergenic
TTGTTCAAAGGAAGGGTTTC+GGG 0.231331 1:-7084029 MsaT000546.1:CDS
GAGATTCGGACTCCTCTTCT+TGG 0.249119 1:-7082106 MsaT000546.1:CDS
ACCAATATTTGGAAAATATT+CGG 0.254322 1:-7082180 MsaT000546.1:CDS
TCCTCTTCTTGGGTTGAATA+AGG 0.262656 1:-7082095 MsaT000546.1:CDS
CATTCCTTCAAAGGGCTCTA+AGG 0.274189 1:+7084180 None:intergenic
TGACACTCTTATTTGATTCA+TGG 0.286816 1:-7082267 MsaT000546.1:CDS
TGATGCACATGACATTATAT+TGG 0.315744 1:-7082300 MsaT000546.1:CDS
GATGCACATGACATTATATT+GGG 0.333053 1:-7082299 MsaT000546.1:CDS
CGTTTGGTTACTTCTTGTTT+AGG 0.336421 1:-7081949 MsaT000546.1:CDS
AGAACAGACGGAATGATAAT+TGG 0.340924 1:-7084064 MsaT000546.1:CDS
ATTCATGGAAAACCAATTCA+TGG 0.342364 1:-7082252 MsaT000546.1:CDS
TTGGCTATAATGTCGTTTAA+TGG 0.343696 1:-7082004 MsaT000546.1:CDS
ATGTCAAGAGCAGCAGTTTC+AGG 0.356702 1:+7084238 None:intergenic
TTTGGTGTGAATTCTGCTAT+TGG 0.364233 1:-7082040 MsaT000546.1:CDS
ATTGTGGTTGGTTTAACGTT+TGG 0.388729 1:-7081965 MsaT000546.1:CDS
TAGATAACTCGTCCATGAAT+TGG 0.393378 1:+7082240 None:intergenic
AGATTCGGACTCCTCTTCTT+GGG 0.397569 1:-7082105 MsaT000546.1:CDS
TTGGTGTGAATTCTGCTATT+GGG 0.404245 1:-7082039 MsaT000546.1:CDS
ATTTGGAAAATATTCGGATT+CGG 0.414816 1:-7082174 MsaT000546.1:CDS
AGTCGCCGGTGCCGGAGATT+CGG 0.431491 1:-7082120 MsaT000546.1:CDS
TATTGGGTATTTGTTGATGT+TGG 0.437573 1:-7082023 MsaT000546.1:CDS
TTTGTTCAAAGGAAGGGTTT+CGG 0.447784 1:-7084030 MsaT000546.1:CDS
GAGGAACAAGGTTGTTGAAT+CGG 0.457237 1:-7082068 MsaT000546.1:CDS
AATGTCATGTGCATCATCTT+CGG 0.473949 1:+7082307 None:intergenic
CTTAGAGCCCTTTGAAGGAA+TGG 0.476103 1:-7084179 MsaT000546.1:CDS
AGGCAAGGAATCACTACAAA+AGG 0.478706 1:-7084138 MsaT000546.1:CDS
TTGGGTTGAATAAGGTTTCG+AGG 0.486516 1:-7082087 MsaT000546.1:CDS
ATTCCTTCAAAGGGCTCTAA+GGG 0.488264 1:+7084181 None:intergenic
AGAAGAGGAGTCCGAATCTC+CGG 0.488903 1:+7082109 None:intergenic
TGTGAACTCCATTCCTTCAA+AGG 0.507742 1:+7084171 None:intergenic
GAGGACGCAGTCGCCGGTGC+CGG 0.509370 1:-7082128 MsaT000546.1:CDS
GTGAACTCCATTCCTTCAAA+GGG 0.518109 1:+7084172 None:intergenic
TGAGGGGAGGACGCAGTCGC+CGG 0.519658 1:-7082134 MsaT000546.1:CDS
GTGATTCCTTGCCTCTTCGA+TGG 0.520594 1:+7084147 None:intergenic
TAAAGAGTTTCTTTGTTCAA+AGG 0.523442 1:-7084041 MsaT000546.1:CDS
GAGTTTCTTTGTTCAAAGGA+AGG 0.524715 1:-7084037 MsaT000546.1:CDS
GTGTCAGACATGATGAAACT+AGG 0.525439 1:-7083973 MsaT000546.1:CDS
AGTTTCTTTGTTCAAAGGAA+GGG 0.533222 1:-7084036 MsaT000546.1:CDS
ACCTTATTCAACCCAAGAAG+AGG 0.545808 1:+7082094 None:intergenic
GGAGTCCGAATCTCCGGCAC+CGG 0.550760 1:+7082115 None:intergenic
TCAGAGTGAAGAAGAGGCTG+CGG 0.552122 1:-7081924 MsaT000546.1:CDS
TGAAACTGCTGCTCTTGACA+TGG 0.571525 1:-7084236 MsaT000546.1:CDS
CTGTTCTTGATTTAGTAACA+CGG 0.577898 1:+7084080 None:intergenic
TAGGAGTCAGAGTGAAGAAG+AGG 0.579273 1:-7081930 MsaT000546.1:CDS
CACAAACTAAGCACATGCAC+AGG 0.579857 1:+7081886 None:intergenic
GAATAAGGTTTCGAGGAACA+AGG 0.583225 1:-7082080 MsaT000546.1:CDS
AGTAACACGGCCCATGCGAA+AGG 0.583261 1:+7084093 None:intergenic
TGTCAGACATGATGAAACTA+GGG 0.602315 1:-7083972 MsaT000546.1:intron
GCTATAATGTCGTTTAATGG+TGG 0.610087 1:-7082001 MsaT000546.1:CDS
GTTACTAAATCAAGAACAGA+CGG 0.612323 1:-7084076 MsaT000546.1:CDS
TACATCATCAGAACAAGCCA+TGG 0.620956 1:+7084261 None:intergenic
TCACATCCATCGAAGAGGCA+AGG 0.624553 1:-7084153 MsaT000546.1:CDS
AAAAGGTACGCTAAGAACAA+AGG 0.638535 1:-7084121 MsaT000546.1:CDS
TCCTTCAAAGGGCTCTAAGG+GGG 0.640867 1:+7084183 None:intergenic
ATCATCAGAACAAGCCATGG+TGG 0.649034 1:+7084264 None:intergenic
CAGATTAAAGATGACAGATG+TGG 0.651464 1:-7084296 None:intergenic
ATGCACATGACATTATATTG+GGG 0.668922 1:-7082298 MsaT000546.1:CDS
TTCCTTCAAAGGGCTCTAAG+GGG 0.673359 1:+7084182 None:intergenic
GGAGTTCACATCCATCGAAG+AGG 0.673976 1:-7084158 MsaT000546.1:CDS
TGTGGATGAAGATTCCACCA+TGG 0.737062 1:-7084278 MsaT000546.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr1 gene 7081892 7084308 7081892 ID=MsaG000546
Chr1 mRNA 7081892 7084308 7081892 ID=MsaT000546.1;Parent=MsaG000546
Chr1 exon 7081892 7082422 7081892 ID=MsaT000546.1.exon2;Parent=MsaT000546.1
Chr1 CDS 7081892 7082422 7081892 ID=cds.MsaT000546.1;Parent=MsaT000546.1
Chr1 exon 7083973 7084308 7083973 ID=MsaT000546.1.exon1;Parent=MsaT000546.1
Chr1 CDS 7083973 7084308 7083973 ID=cds.MsaT000546.1;Parent=MsaT000546.1
Gene Sequence

>MsaT000546.1

ATGACAGATGTGGATGAAGATTCCACCATGGCTTGTTCTGATGATGTACCTGAAACTGCTGCTCTTGACATGGATCCTATAAATAACGAACTACCATTAGAGTCCCCCTTAGAGCCCTTTGAAGGAATGGAGTTCACATCCATCGAAGAGGCAAGGAATCACTACAAAAGGTACGCTAAGAACAAAGGTTTTACCTTTCGCATGGGCCGTGTTACTAAATCAAGAACAGACGGAATGATAATTGGTAAAGAGTTTCTTTGTTCAAAGGAAGGGTTTCGGGCAAAAAAATATGCAAAGAAAGAAAATAGCAGTCGTGTCAGACATGATGAAACTAGGAAACACACACAAAAAACAAAAACCTTGAAATTTTCAAAACAACCTTGTTCGTTTTTCCGACATAGATCGGAAAATTTCAAAACCTTAATTTTTCCGAAGATGATGCACATGACATTATATTGGGGAAAAAACGTGACACTCTTATTTGATTCATGGAAAACCAATTCATGGACGAGTTATCTACTGAGTTTAATTGCATGTTTCATGATTGCAACATTCTACCAATATTTGGAAAATATTCGGATTCGGTTGAAGTTTTTCTCTGGTGAGGGGAGGACGCAGTCGCCGGTGCCGGAGATTCGGACTCCTCTTCTTGGGTTGAATAAGGTTTCGAGGAACAAGGTTGTTGAATCGGTTTTATTTGGTGTGAATTCTGCTATTGGGTATTTGTTGATGTTGGCTATAATGTCGTTTAATGGTGGTGTTTTTGTGGCTATTGTGGTTGGTTTAACGTTTGGTTACTTCTTGTTTAGGAGTCAGAGTGAAGAAGAGGCTGCGGTTGTTGTTGATAATTCCTGTGCATGTGCTTAG

Protein sequence

>MsaT000546.1

MTDVDEDSTMACSDDVPETAALDMDPINNELPLESPLEPFEGMEFTSIEEARNHYKRYAKNKGFTFRMGRVTKSRTDGMIIGKEFLCSKEGFRAKKYAKKENSSRVRHDETRKHTQKTKTLKFSKQPCSFFRHRSENFKTLIFPKMMHMTLYWGKNVTLLFDSWKTNSWTSYLLSLIACFMIATFYQYLENIRIRLKFFSGEGRTQSPVPEIRTPLLGLNKVSRNKVVESVLFGVNSAIGYLLMLAIMSFNGGVFVAIVVGLTFGYFLFRSQSEEEAAVVVDNSCACA*