AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG000563

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG000563 MtrunA17_Chr1g0150511 98.516 1011 14 1 1 1010 1 1011 0.0 2070
MsaG000563 MtrunA17_Chr3g0136601 68.830 1017 239 14 5 1011 1 949 0.0 1355
MsaG000563 MtrunA17_Chr1g0150531 82.707 399 58 3 43 441 2 389 0.0 656
MsaG000563 MtrunA17_Chr3g0122391 35.535 318 170 7 379 695 443 726 1.13e-41 166
MsaG000563 MtrunA17_Chr3g0122391 32.639 144 77 3 869 1011 901 1025 4.10e-13 73.9
MsaG000563 MtrunA17_Chr8g0377481 34.323 303 162 6 379 680 395 661 3.90e-38 154
MsaG000563 MtrunA17_Chr8g0392301 34.603 315 179 8 379 686 386 680 6.73e-38 154
MsaG000563 MtrunA17_Chr8g0392301 32.934 167 96 4 846 1011 844 995 1.92e-16 84.7
MsaG000563 MtrunA17_Chr5g0447641 32.237 304 173 6 379 680 620 892 5.02e-35 145
MsaG000563 MtrunA17_Chr5g0447641 31.429 140 89 2 858 996 1065 1198 2.01e-12 71.6
MsaG000563 MtrunA17_Chr4g0013831 38.095 147 88 2 380 524 348 493 4.17e-24 108
MsaG000563 MtrunA17_Chr8g0371601 28.000 300 167 6 379 676 474 726 4.32e-24 109
MsaG000563 MtrunA17_Chr8g0371601 28.346 254 124 8 773 1004 942 1159 2.13e-11 68.6
MsaG000563 MtrunA17_Chr5g0405701 29.085 306 168 6 379 682 512 770 5.67e-24 109
MsaG000563 MtrunA17_Chr5g0405701 29.592 196 82 4 832 999 1018 1185 7.27e-11 66.6
MsaG000563 MtrunA17_Chr7g0269631 37.226 137 83 2 380 514 348 483 3.67e-17 86.7
MsaG000563 MtrunA17_Chr7g0269631 34.559 136 83 3 585 717 484 616 1.73e-13 74.7
MsaG000563 MtrunA17_Chr1g0197591 25.956 366 204 12 328 680 337 648 8.51e-16 82.8
MsaG000563 MtrunA17_Chr1g0194741 25.839 298 160 10 381 662 400 652 2.80e-12 70.9
MsaG000563 MtrunA17_Chr1g0190021 25.641 195 131 4 337 521 327 517 3.75e-11 67.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG000563 AT5G10720.1 55.902 1025 333 17 1 1010 1 921 0.0 1058
MsaG000563 AT5G10680.2 50.581 172 72 4 5 174 1 161 8.27e-41 150
MsaG000563 AT5G10680.1 50.581 172 72 4 5 174 1 161 8.27e-41 150
MsaG000563 AT2G01830.2 33.333 309 170 6 379 680 475 754 1.79e-37 153
MsaG000563 AT2G01830.2 32.558 129 68 3 869 996 947 1057 2.93e-11 68.6
MsaG000563 AT2G01830.1 33.333 309 170 6 379 680 452 731 2.07e-37 152
MsaG000563 AT2G01830.1 32.558 129 68 3 869 996 924 1034 2.76e-11 68.6
MsaG000563 AT2G01830.4 33.333 309 170 6 379 680 452 731 2.07e-37 152
MsaG000563 AT2G01830.4 32.558 129 68 3 869 996 924 1034 2.76e-11 68.6
MsaG000563 AT2G01830.6 33.333 309 170 6 379 680 452 731 2.07e-37 152
MsaG000563 AT2G01830.6 32.558 129 68 3 869 996 924 1034 2.76e-11 68.6
MsaG000563 AT2G01830.3 33.333 309 170 6 379 680 452 731 2.07e-37 152
MsaG000563 AT2G01830.3 32.558 129 68 3 869 996 924 1034 2.76e-11 68.6
MsaG000563 AT2G01830.5 33.333 309 170 6 379 680 464 743 2.40e-37 152
MsaG000563 AT2G01830.5 32.558 129 68 3 869 996 936 1046 2.78e-11 68.6
MsaG000563 AT1G27320.1 33.993 303 161 6 379 680 453 717 4.07e-37 152
MsaG000563 AT1G27320.1 33.083 133 82 3 865 996 888 1014 5.98e-13 73.9
MsaG000563 AT5G35750.1 33.550 307 164 6 379 680 590 861 8.02e-34 141
MsaG000563 AT5G35750.1 33.333 129 79 3 869 996 1037 1159 3.02e-12 71.6
MsaG000563 AT2G17820.1 25.000 396 226 11 309 697 443 774 2.99e-25 114
MsaG000563 AT1G66340.1 37.584 149 91 2 372 518 338 486 9.10e-23 105
MsaG000563 AT2G47430.1 27.536 345 194 12 347 680 366 665 4.27e-19 94.0
MsaG000563 AT2G40940.1 29.545 220 121 6 338 524 282 500 1.78e-16 84.7

Find 178 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAAAATGCACCTGTTGTTAT+TGG 0.158249 1:+7410998 MsaT000563.1:CDS
AACTTTCCTGCTTAGTTTCT+AGG 0.181316 1:-7414487 None:intergenic
GAGATAAGATCGCCACTTTC+TGG 0.209695 1:+7412356 MsaT000563.1:CDS
TTGGTGAGATTCTTCTTCTT+TGG 0.219070 1:-7413349 None:intergenic
GGAAGTTGAGCAAAGAAAAT+TGG 0.237386 1:+7410616 MsaT000563.1:CDS
TTGATCACATCTCATACTTC+TGG 0.237521 1:-7413439 None:intergenic
TTTGGAACAGGTTGAATTAA+TGG 0.245842 1:+7414091 MsaT000563.1:intron
CATTGTTAAACGCATGATTC+TGG 0.255787 1:-7413500 None:intergenic
ACTGGTCTTCTGTGACCTTT+TGG 0.259087 1:-7413368 None:intergenic
TAAAGAGCAAAGACAGCTTT+TGG 0.270075 1:+7412421 MsaT000563.1:CDS
CTTACAAATTATACAGATAA+AGG 0.272256 1:+7410491 MsaT000563.1:CDS
AAAGTTATTTCCTTCTTTGC+AGG 0.284917 1:-7411889 None:intergenic
CATGTTATCTTCAGGAGATT+TGG 0.286514 1:+7412448 MsaT000563.1:CDS
AACATATCTTGGTCTCTTCC+TGG 0.287184 1:-7410410 None:intergenic
AATAAAACCATTCACATTAC+AGG 0.292769 1:+7412186 MsaT000563.1:CDS
CCAATTCCAACCACGAACTT+TGG 0.307599 1:+7414268 MsaT000563.1:CDS
CAAACGTCCAGTTTCGATAT+TGG 0.310025 1:+7410691 MsaT000563.1:CDS
TCTTTGGCAAATGCTGGTTC+TGG 0.312428 1:-7413333 None:intergenic
GCAGACCATGCTCGAAAATA+TGG 0.317830 1:+7413821 MsaT000563.1:CDS
AACAATTGGAGTAAACTATA+TGG 0.319084 1:+7411981 MsaT000563.1:CDS
CAATTCCAACCACGAACTTT+GGG 0.320655 1:+7414269 MsaT000563.1:CDS
AGTATGATACTGTTGTTTAC+TGG 0.324129 1:+7410804 MsaT000563.1:CDS
GTTTGAAGACCATTCATAAC+TGG 0.338515 1:-7414992 None:intergenic
ACATCAGTTCCAACATCTTC+TGG 0.341534 1:-7410362 None:intergenic
ACAAAACCATGATAATAATA+AGG 0.343332 1:+7414526 MsaT000563.1:CDS
TCGGAGAGAGGATATATTAA+AGG 0.347750 1:+7410871 MsaT000563.1:CDS
CTATACCTACTTTATTTAGA+AGG 0.350625 1:+7413783 MsaT000563.1:CDS
ATTCGGCTTTAACGTCGATT+TGG 0.353565 1:-7414708 None:intergenic
TCTTCAACAAGAAGAATATT+CGG 0.358321 1:-7414725 None:intergenic
TTACTTCTTCCAACATATCT+TGG 0.359994 1:-7410421 None:intergenic
GGCTTATTTAATGCAACATT+GGG 0.361294 1:+7410526 MsaT000563.1:CDS
GCAATGGTGCTGCAGCTGTC+TGG 0.366539 1:-7412947 None:intergenic
CCAAAGTTCGTGGTTGGAAT+TGG 0.374939 1:-7414268 None:intergenic
AGCATGGATGTCGTGAATAA+TGG 0.377420 1:+7414800 MsaT000563.1:CDS
TTGGATGTCATGTTATCTTC+AGG 0.380119 1:+7412440 MsaT000563.1:CDS
CATGCACCTTATTATTATCA+TGG 0.383722 1:-7414532 None:intergenic
CTAGAGTTTGCACTACCATT+TGG 0.384833 1:-7414563 None:intergenic
CTTATGAAGAAACAGGCAAT+TGG 0.396625 1:+7415032 MsaT000563.1:CDS
TTCGAGGAAGAGATTAGTTA+TGG 0.399361 1:+7410662 MsaT000563.1:CDS
GCATCGTCCAATATCGAAAC+TGG 0.402160 1:-7410698 None:intergenic
ATTACCTCTTCTTGTAAACT+TGG 0.403224 1:-7411640 None:intergenic
AACAGTTGGAGGCGAGTATT+CGG 0.405955 1:+7410852 MsaT000563.1:CDS
AATTGGAAGCTACAAAATTC+AGG 0.406669 1:+7412898 MsaT000563.1:CDS
GAAGTGTTATATACCTCAAT+AGG 0.406853 1:-7413011 None:intergenic
TGATCACATCTCATACTTCT+GGG 0.407144 1:-7413438 None:intergenic
TGATGATCCAGATGATCTTT+CGG 0.409319 1:+7414205 MsaT000563.1:CDS
ACCTGTTGTTATTGGCCATC+AGG 0.410974 1:+7411006 MsaT000563.1:CDS
AAGAGAGAAGTAATGGAGAA+AGG 0.411442 1:+7411862 MsaT000563.1:CDS
TCTTCTTCTTTGGCAAATGC+TGG 0.415639 1:-7413339 None:intergenic
CAGTCAACTATTTCAGCAAA+TGG 0.416796 1:+7413387 MsaT000563.1:CDS
TCAAGTAACATCATTTCAAA+TGG 0.417988 1:+7414383 MsaT000563.1:CDS
ATGTATAGGAGCTATGAAAT+TGG 0.423052 1:+7412881 MsaT000563.1:intron
ATTAGGTCTTATGAAGAAAC+AGG 0.429646 1:+7415025 MsaT000563.1:CDS
TTGGAACAGGTTGAATTAAT+GGG 0.430242 1:+7414092 MsaT000563.1:intron
GATCTTTCCAAGGTTGAGTC+AGG 0.431218 1:+7412497 MsaT000563.1:CDS
CACATTCATACTACCATACA+AGG 0.433058 1:+7414160 MsaT000563.1:CDS
AAATATGGAGGCACAGGTCT+AGG 0.436640 1:+7413836 MsaT000563.1:CDS
TCGAGGAAGAGATTAGTTAT+GGG 0.438884 1:+7410663 MsaT000563.1:CDS
AAGATCTACCGGCGGCGATA+AGG 0.439631 1:+7410735 MsaT000563.1:CDS
TCAAAATAATGTCGTATGAA+TGG 0.440366 1:-7414850 None:intergenic
TTCAAACAACAAAACTGATT+AGG 0.443792 1:+7415008 MsaT000563.1:CDS
AGATAAAGGGTCATCTCAAT+TGG 0.444089 1:+7410505 MsaT000563.1:CDS
ATAAGCAGGCGAACGCGGTT+CGG 0.446744 1:+7410552 MsaT000563.1:CDS
CAAGCTGTTGAAACCTTGTA+TGG 0.450617 1:-7414173 None:intergenic
TGGGATGCTGCAAGAGAAGC+TGG 0.451002 1:+7415052 MsaT000563.1:CDS
ATGTAAATATTGCAGGTCAC+TGG 0.451028 1:+7413089 MsaT000563.1:intron
CGAGGAAGAGATTAGTTATG+GGG 0.452682 1:+7410664 MsaT000563.1:CDS
TCAGGCCAAGAGAGGTAGTA+AGG 0.452778 1:+7412916 MsaT000563.1:CDS
TGGCTTATTTAATGCAACAT+TGG 0.452961 1:+7410525 MsaT000563.1:CDS
AAATGGAACAAATTCAATTG+AGG 0.453149 1:+7414400 MsaT000563.1:CDS
ACTAGCAATATGTAAACAAC+TGG 0.455705 1:+7413859 MsaT000563.1:CDS
TTGAAATGATGTTACTTGAA+TGG 0.456614 1:-7414379 None:intergenic
AGTGTCTTGAAAAGTATCTA+AGG 0.457868 1:+7415460 MsaT000563.1:CDS
AGATTCCTTCACACCTGCTC+CGG 0.458251 1:-7411830 None:intergenic
CTGGTTCTGGAACAACATAA+AGG 0.460292 1:-7413320 None:intergenic
TCGACATCCGAAAGATCATC+TGG 0.460612 1:-7414212 None:intergenic
CAGAAAATGTTAACATTGGA+AGG 0.461173 1:+7412969 MsaT000563.1:CDS
ACAATTGGAGTAAACTATAT+GGG 0.461326 1:+7411982 MsaT000563.1:CDS
AGCAAGGCAGGTGAAACAAT+TGG 0.462061 1:+7411967 MsaT000563.1:CDS
GATTAAACCTGTAATGTGAA+TGG 0.463646 1:-7412193 None:intergenic
GAAAGGAGTGCCTGCAAAGA+AGG 0.466099 1:+7411879 MsaT000563.1:CDS
CTCTACTTGCTTCATTACAT+TGG 0.472879 1:-7414586 None:intergenic
AGATTCTTACAAATTTAGTC+AGG 0.474911 1:+7413132 MsaT000563.1:CDS
ACATGCCTTACTACCTCTCT+TGG 0.479070 1:-7412921 None:intergenic
CTATACATGGCAAGATCTAC+CGG 0.482979 1:+7410724 MsaT000563.1:CDS
TGAACCACAACAAACATGTC+AGG 0.483397 1:+7414634 MsaT000563.1:CDS
GTTTACATGCCAGTTATGAA+TGG 0.483418 1:+7414983 MsaT000563.1:CDS
AAGCAGGAAAGTTTATGTAA+TGG 0.484176 1:+7414497 MsaT000563.1:CDS
AGAATGTTTCGCCAACGGTA+TGG 0.484664 1:+7415395 MsaT000563.1:CDS
ACCTGATGGCCAATAACAAC+AGG 0.484985 1:-7411007 None:intergenic
ACTTTCTGGTGTTGTTAGCA+TGG 0.486916 1:+7412370 MsaT000563.1:CDS
TTACAAATTATACAGATAAA+GGG 0.487288 1:+7410492 MsaT000563.1:CDS
TTATGAAGAAACAGGCAATT+GGG 0.491132 1:+7415033 MsaT000563.1:CDS
GGGAATGGAAATAACAGATC+AGG 0.491945 1:+7412002 MsaT000563.1:CDS
AGAATCTCACACAAGTTCAA+TGG 0.492632 1:+7414329 MsaT000563.1:CDS
AAACAAGAGAATTGAAATTG+AGG 0.492797 1:+7410778 MsaT000563.1:CDS
TATTTCAGCAAATGGATTGA+AGG 0.494958 1:+7413395 MsaT000563.1:CDS
GAACCTGTGTTTAGCAAGGC+AGG 0.495935 1:+7411955 MsaT000563.1:CDS
CTTTGAAAATCAGCTATAGC+TGG 0.498599 1:-7410458 None:intergenic
GATGTGTACGACACTGGAAT+TGG 0.499123 1:+7413615 MsaT000563.1:CDS
GAGAAAGAATGGCAAAGCTA+AGG 0.499190 1:+7412124 MsaT000563.1:CDS
TTTCTAACCTGACTCAACCT+TGG 0.509156 1:-7412504 None:intergenic
AGATAACAAGATTAATATCA+TGG 0.517181 1:+7414745 MsaT000563.1:CDS
GTTTGAAATGGTTTGTGAGA+TGG 0.519841 1:+7410286 None:intergenic
GTGTGAGATTCTATGAGGCC+TGG 0.520618 1:-7414318 None:intergenic
TAAAGAGAATAATCGATTCG+AGG 0.522514 1:+7410646 MsaT000563.1:CDS
AGGAAGAGACCAAGATATGT+TGG 0.524319 1:+7410412 MsaT000563.1:CDS
ATTGCAGAAAATGTTAACAT+TGG 0.525478 1:+7412965 MsaT000563.1:CDS
GAAGATGTTGGAACTGATGT+TGG 0.527223 1:+7410365 MsaT000563.1:CDS
AGAAGAAGAATCTCACCAAA+AGG 0.528810 1:+7413353 MsaT000563.1:CDS
AATGAGAGCAAAACAAATGC+TGG 0.532235 1:+7412319 MsaT000563.1:CDS
ATGGCTCTGAACTGCGCGTA+TGG 0.532419 1:-7414831 None:intergenic
TTTACACATCAAGGCAAAGT+TGG 0.534123 1:+7413291 MsaT000563.1:CDS
GCTCGAAAATATGGAGGCAC+AGG 0.535185 1:+7413830 MsaT000563.1:CDS
CATTTGCTGAAATAGTTGAC+TGG 0.538486 1:-7413386 None:intergenic
ATCTGTGACAGTTCCTCCAC+AGG 0.539752 1:-7410938 None:intergenic
GCTGTCAAGTTTACACATCA+AGG 0.539918 1:+7413282 MsaT000563.1:CDS
TGATATACTTGATCTTTCCA+AGG 0.542061 1:+7412487 MsaT000563.1:CDS
AGCCGATGATATACCTATTG+AGG 0.543862 1:+7412998 MsaT000563.1:CDS
AAAAGCGAGCCCAAAGTTCG+TGG 0.544177 1:-7414278 None:intergenic
TTTGAGTAAGAATCGTTGTC+CGG 0.544394 1:-7414356 None:intergenic
TGGACGATGCTTACTATACA+TGG 0.547039 1:+7410711 MsaT000563.1:CDS
ATGCTAACAACACCAGAAAG+TGG 0.550524 1:-7412368 None:intergenic
AACAAACATGTCAGGGTCAA+GGG 0.550696 1:+7414642 MsaT000563.1:CDS
GCGAGCCCAAAGTTCGTGGT+TGG 0.551447 1:-7414274 None:intergenic
TACAAAATTCAGGCCAAGAG+AGG 0.552848 1:+7412908 MsaT000563.1:CDS
TGGAGTAAACTATATGGGAA+TGG 0.555183 1:+7411987 MsaT000563.1:CDS
GTGCATGATAGCTTACCAAA+TGG 0.555791 1:+7414548 MsaT000563.1:CDS
GACACTGGAATTGGCATACC+AGG 0.557084 1:+7413624 MsaT000563.1:CDS
GCAACATTGGGAGTATAAGC+AGG 0.560108 1:+7410538 MsaT000563.1:CDS
TGACCCTGACATGTTTGTTG+TGG 0.560232 1:-7414638 None:intergenic
AGAAAGAATGGCAAAGCTAA+GGG 0.561686 1:+7412125 MsaT000563.1:CDS
TGGAACAGGTTGAATTAATG+GGG 0.566185 1:+7414093 MsaT000563.1:intron
CAACAAACATGTCAGGGTCA+AGG 0.567768 1:+7414641 MsaT000563.1:CDS
CAATCTTAGCAAACAAAGGA+AGG 0.568976 1:+7410595 MsaT000563.1:CDS
CACAAATGCGTCCATACCGT+TGG 0.570023 1:-7415406 None:intergenic
ACGCATGATTCTGGATAGGA+TGG 0.570519 1:-7413491 None:intergenic
CGTTGTGATGTGTACGACAC+TGG 0.576348 1:+7413609 MsaT000563.1:CDS
ATGTACCTTCTAAATAAAGT+AGG 0.578318 1:-7413788 None:intergenic
TCACAGCCTAGAAACTAAGC+AGG 0.578579 1:+7414481 MsaT000563.1:CDS
AACTTGTGTGAGATTCTATG+AGG 0.581575 1:-7414323 None:intergenic
TTAAACGCATGATTCTGGAT+AGG 0.582689 1:-7413495 None:intergenic
TCACTTCTAATGGATCCACC+AGG 0.589068 1:+7414300 MsaT000563.1:CDS
TCACCTGCCTTGCTAAACAC+AGG 0.591633 1:-7411958 None:intergenic
AAGTCAATGATGAAGCAGCT+AGG 0.595119 1:+7414773 MsaT000563.1:CDS
GAGGAAGAGATTAGTTATGG+GGG 0.595191 1:+7410665 MsaT000563.1:CDS
TACCTCAATAGGTATATCAT+CGG 0.595988 1:-7413000 None:intergenic
CATCTCATACTTCTGGGTGA+TGG 0.595990 1:-7413432 None:intergenic
TTTCACCGGAGCAGGTGTGA+AGG 0.596051 1:+7411825 MsaT000563.1:CDS
AACATGTCAGGGTCAAGGGA+AGG 0.598087 1:+7414646 MsaT000563.1:CDS
GGAGGCGAGTATTCGGAGAG+AGG 0.602582 1:+7410859 MsaT000563.1:CDS
TTGACAATCTTAGCAAACAA+AGG 0.607034 1:+7410591 MsaT000563.1:CDS
TGTAGAACCTGTGTTTAGCA+AGG 0.614185 1:+7411951 MsaT000563.1:CDS
TGATTGTATTGAAGACATGA+TGG 0.615316 1:+7410313 MsaT000563.1:CDS
TTGGAATTGGAAGAATCCCT+CGG 0.616918 1:-7414255 None:intergenic
GAAGCAGCTAGGATATAGCA+TGG 0.617920 1:+7414784 MsaT000563.1:CDS
GCTGAAGAATGTTTCGCCAA+CGG 0.618188 1:+7415390 MsaT000563.1:CDS
TCAATGTGGCCAGAAGATGT+TGG 0.623443 1:+7410353 MsaT000563.1:CDS
GAACCACAACAAACATGTCA+GGG 0.628011 1:+7414635 MsaT000563.1:CDS
CTGTTGTTTACTGGAAACAA+CGG 0.636650 1:+7410813 MsaT000563.1:CDS
GGAACAGGTTGAATTAATGG+GGG 0.643012 1:+7414094 MsaT000563.1:intron
TGAGATTCTATGAGGCCTGG+TGG 0.645843 1:-7414315 None:intergenic
AAGACAATCATCACCTGTGG+AGG 0.647566 1:+7410925 MsaT000563.1:CDS
AATTGCAGTTCAGAAAGCAA+AGG 0.648370 1:+7412152 MsaT000563.1:CDS
GGAGTATAAGCAGGCGAACG+CGG 0.650670 1:+7410547 MsaT000563.1:CDS
TGAAAGACAATCATCACCTG+TGG 0.652639 1:+7410922 MsaT000563.1:CDS
AAAGCGCATCCACATTGTTG+CGG 0.653392 1:+7415123 MsaT000563.1:CDS
TTGAAGAGTTATCTTCAATG+TGG 0.654257 1:+7410339 MsaT000563.1:CDS
AACGAAGATGACACAACCGA+GGG 0.659560 1:+7414239 MsaT000563.1:CDS
GACCATGCTCGAAAATATGG+AGG 0.667505 1:+7413824 MsaT000563.1:CDS
GAACAGGTTGAATTAATGGG+GGG 0.672744 1:+7414095 MsaT000563.1:intron
GGATGTCGTGAATAATGGAG+TGG 0.687300 1:+7414805 MsaT000563.1:CDS
TAACGAAGATGACACAACCG+AGG 0.691038 1:+7414238 MsaT000563.1:CDS
GTGACTAGCAAAGAACATTG+TGG 0.699673 1:+7414128 MsaT000563.1:CDS
GTATGGTAGTATGAATGTGA+AGG 0.713387 1:-7414156 None:intergenic
AATTGCTCACCGCAACAATG+TGG 0.726029 1:-7415132 None:intergenic
ATTCAACTGAAACAACAGTG+TGG 0.734063 1:+7413583 MsaT000563.1:CDS
TACATGGCAAGATCTACCGG+CGG 0.803486 1:+7410727 MsaT000563.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr1 gene 7410293 7415485 7410293 ID=MsaG000563
Chr1 mRNA 7410293 7415485 7410293 ID=MsaT000563.1;Parent=MsaG000563
Chr1 exon 7410293 7411027 7410293 ID=MsaT000563.1.exon1;Parent=MsaT000563.1
Chr1 CDS 7410293 7411027 7410293 ID=cds.MsaT000563.1;Parent=MsaT000563.1
Chr1 exon 7411601 7411657 7411601 ID=MsaT000563.1.exon2;Parent=MsaT000563.1
Chr1 CDS 7411601 7411657 7411601 ID=cds.MsaT000563.1;Parent=MsaT000563.1
Chr1 exon 7411796 7412023 7411796 ID=MsaT000563.1.exon3;Parent=MsaT000563.1
Chr1 CDS 7411796 7412023 7411796 ID=cds.MsaT000563.1;Parent=MsaT000563.1
Chr1 exon 7412114 7412207 7412114 ID=MsaT000563.1.exon4;Parent=MsaT000563.1
Chr1 CDS 7412114 7412207 7412114 ID=cds.MsaT000563.1;Parent=MsaT000563.1
Chr1 exon 7412312 7412518 7412312 ID=MsaT000563.1.exon5;Parent=MsaT000563.1
Chr1 CDS 7412312 7412518 7412312 ID=cds.MsaT000563.1;Parent=MsaT000563.1
Chr1 exon 7412889 7413019 7412889 ID=MsaT000563.1.exon6;Parent=MsaT000563.1
Chr1 CDS 7412889 7413019 7412889 ID=cds.MsaT000563.1;Parent=MsaT000563.1
Chr1 exon 7413104 7413153 7413104 ID=MsaT000563.1.exon7;Parent=MsaT000563.1
Chr1 CDS 7413104 7413153 7413104 ID=cds.MsaT000563.1;Parent=MsaT000563.1
Chr1 exon 7413278 7413645 7413278 ID=MsaT000563.1.exon8;Parent=MsaT000563.1
Chr1 CDS 7413278 7413645 7413278 ID=cds.MsaT000563.1;Parent=MsaT000563.1
Chr1 exon 7413777 7413880 7413777 ID=MsaT000563.1.exon9;Parent=MsaT000563.1
Chr1 CDS 7413777 7413880 7413777 ID=cds.MsaT000563.1;Parent=MsaT000563.1
Chr1 exon 7414101 7414874 7414101 ID=MsaT000563.1.exon10;Parent=MsaT000563.1
Chr1 CDS 7414101 7414874 7414101 ID=cds.MsaT000563.1;Parent=MsaT000563.1
Chr1 exon 7414980 7415144 7414980 ID=MsaT000563.1.exon11;Parent=MsaT000563.1
Chr1 CDS 7414980 7415144 7414980 ID=cds.MsaT000563.1;Parent=MsaT000563.1
Chr1 exon 7415363 7415485 7415363 ID=MsaT000563.1.exon12;Parent=MsaT000563.1
Chr1 CDS 7415363 7415485 7415363 ID=cds.MsaT000563.1;Parent=MsaT000563.1
Gene Sequence

>MsaT000563.1

ATGGTTTGTGAGATGGAGAGTGATTGTATTGAAGACATGATGGACATTGAAGAGTTATCTTCAATGTGGCCAGAAGATGTTGGAACTGATGTTGGAAAACAATTCAACATAGAAAAACCAGGAAGAGACCAAGATATGTTGGAAGAAGTAACAATCTTAGAAGAACCAGCTATAGCTGATTTTCAAAGACTAATGGAGCTTACAAATTATACAGATAAAGGGTCATCTCAATTGGCTTATTTAATGCAACATTGGGAGTATAAGCAGGCGAACGCGGTTCGGCTTCTTCGAGAAGAGCTTGACAATCTTAGCAAACAAAGGAAGGAAGTTGAGCAAAGAAAATTGGAGATACTTAAAGAGAATAATCGATTCGAGGAAGAGATTAGTTATGGGGGAGACAAACGTCCAGTTTCGATATTGGACGATGCTTACTATACATGGCAAGATCTACCGGCGGCGATAAGGAAAAGTGATGTTGTTGTTCAAAACAAGAGAATTGAAATTGAGGCTGAGTATGATACTGTTGTTTACTGGAAACAACGGTCGCAAGATTTTGAAAAACAGTTGGAGGCGAGTATTCGGAGAGAGGATATATTAAAGGAGAAATTGCAAGAAAGTATAGAGACTATTGAAAGACAATCATCACCTGTGGAGGAACTGTCACAGATTCTTAAGAGAGCAGATAATTTCTTACATTTTATACTTCAAAATGCACCTGTTGTTATTGGCCATCAGGACAAAGAGTTGCGCTATCGTTTTATCTATAATCATTTTCCAAGTTTACAAGAAGAGGACATTATAGGAAAAACAGATGTCGAAATTTTCACCGGAGCAGGTGTGAAGGAATCTCAAGATTTTAAGAGAGAAGTAATGGAGAAAGGAGTGCCTGCAAAGAAGGAAATAACTTTTGAGACAGAACTATTTGGTTTTAAGACATTTTTGATATATGTAGAACCTGTGTTTAGCAAGGCAGGTGAAACAATTGGAGTAAACTATATGGGAATGGAAATAACAGATCAGGTGAGAAAAAGAGAAAGAATGGCAAAGCTAAGGGAAGAAATTGCAGTTCAGAAAGCAAAGGAAACAGAACTTAATAAAACCATTCACATTACAGAGGAGACAATGAGAGCAAAACAAATGCTGGCAACAATGTCGCATGAGATAAGATCGCCACTTTCTGGTGTTGTTAGCATGGCTGAAATTCTTACGACAACAAAACTTGATAAAGAGCAAAGACAGCTTTTGGATGTCATGTTATCTTCAGGAGATTTGGTTCTTCAACTTATAAATGATATACTTGATCTTTCCAAGGTTGAGTCAGGAGCTATGAAATTGGAAGCTACAAAATTCAGGCCAAGAGAGGTAGTAAGGCATGTACTCCAGACAGCTGCAGCACCATTGCAGAAAATGTTAACATTGGAAGGAAATGTAGCCGATGATATACCTATTGAGGTCACTGGAGATGTTTTAAGGATTCGGCAGATTCTTACAAATTTAGTCAGCAATGCTGTCAAGTTTACACATCAAGGCAAAGTTGGTATAAACCTTTATGTTGTTCCAGAACCAGCATTTGCCAAAGAAGAAGAATCTCACCAAAAGGTCACAGAAGACCAGTCAACTATTTCAGCAAATGGATTGAAGGAAGATAAACATACACCATCACCCAGAAGTATGAGATGTGATCAAAATCATATAGATGATAGAAAACACGCTGACCATCCTATCCAGAATCATGCGTTTAACAATGAGTGTAGATCTTCAGTTAACAGTGAATGCTCAATGAATGATGATACTGAAGAACAAACTCATTCAACTGAAACAACAGTGTGGATACGTTGTGATGTGTACGACACTGGAATTGGCATACCAGAAAAGGCTATACCTACTTTATTTAGAAGGTACATGCAAGTTAGTGCAGACCATGCTCGAAAATATGGAGGCACAGGTCTAGGACTAGCAATATGTAAACAACTGGTTGAATTAATGGGGGGTCGTCTAACAGTGACTAGCAAAGAACATTGTGGTTCTACCTTCACATTCATACTACCATACAAGGTTTCAACAGCTTGTGAAAATTCTGATGATCCAGATGATCTTTCGGATGTCGATAATAACGAAGATGACACAACCGAGGGATTCTTCCAATTCCAACCACGAACTTTGGGCTCGCTTTTCACTTCTAATGGATCCACCAGGCCTCATAGAATCTCACACAAGTTCAATGGTTTTCCGGACAACGATTCTTACTCAAACCATTCAAGTAACATCATTTCAAATGGAACAAATTCAATTGAGGATGCATCTTCTGTTATTGTTGATGCTTCAGATATGTCAGAATCAACAAATTCGTTTAGTCACAGCCTAGAAACTAAGCAGGAAAGTTTATGTAATGGAAACAAACAAAACCATGATAATAATAAGGTGCATGATAGCTTACCAAATGGTAGTGCAAACTCTAGCCAATGTAATGAAGCAAGTAGAGAAATGAATTTAGAAACAAAGTCAAATGAACCACAACAAACATGTCAGGGTCAAGGGAAGGAAGATAGCACTACTAGCACTAGCACATCATCAGAAGTAACCAAATCGACGTTAAAGCCGAATATTCTTCTTGTTGAAGATAACAAGATTAATATCATGGTGACAAAGTCAATGATGAAGCAGCTAGGATATAGCATGGATGTCGTGAATAATGGAGTGGAAGCCATACGCGCAGTTCAGAGCCATTCATACGACATTATTTTGATGGATGTTTACATGCCAGTTATGAATGGTCTTCAAACAACAAAACTGATTAGGTCTTATGAAGAAACAGGCAATTGGGATGCTGCAAGAGAAGCTGGAGTTGAACAAAGTATATCAGCATCAGATGAATGTTCTGTGCCCCCTAAAAAGCGCATCCACATTGTTGCGATGACAGCAAACACAATGTCAGAGAGTGCTGAAGAATGTTTCGCCAACGGTATGGACGCATTTGTGTCAAAACCAGTGACATTCCTAAAATTGAAAGAGTGTCTTGAAAAGTATCTAAGGTGA
Protein sequence

>MsaT000563.1

MVCEMESDCIEDMMDIEELSSMWPEDVGTDVGKQFNIEKPGRDQDMLEEVTILEEPAIADFQRLMELTNYTDKGSSQLAYLMQHWEYKQANAVRLLREELDNLSKQRKEVEQRKLEILKENNRFEEEISYGGDKRPVSILDDAYYTWQDLPAAIRKSDVVVQNKRIEIEAEYDTVVYWKQRSQDFEKQLEASIRREDILKEKLQESIETIERQSSPVEELSQILKRADNFLHFILQNAPVVIGHQDKELRYRFIYNHFPSLQEEDIIGKTDVEIFTGAGVKESQDFKREVMEKGVPAKKEITFETELFGFKTFLIYVEPVFSKAGETIGVNYMGMEITDQVRKRERMAKLREEIAVQKAKETELNKTIHITEETMRAKQMLATMSHEIRSPLSGVVSMAEILTTTKLDKEQRQLLDVMLSSGDLVLQLINDILDLSKVESGAMKLEATKFRPREVVRHVLQTAAAPLQKMLTLEGNVADDIPIEVTGDVLRIRQILTNLVSNAVKFTHQGKVGINLYVVPEPAFAKEEESHQKVTEDQSTISANGLKEDKHTPSPRSMRCDQNHIDDRKHADHPIQNHAFNNECRSSVNSECSMNDDTEEQTHSTETTVWIRCDVYDTGIGIPEKAIPTLFRRYMQVSADHARKYGGTGLGLAICKQLVELMGGRLTVTSKEHCGSTFTFILPYKVSTACENSDDPDDLSDVDNNEDDTTEGFFQFQPRTLGSLFTSNGSTRPHRISHKFNGFPDNDSYSNHSSNIISNGTNSIEDASSVIVDASDMSESTNSFSHSLETKQESLCNGNKQNHDNNKVHDSLPNGSANSSQCNEASREMNLETKSNEPQQTCQGQGKEDSTTSTSTSSEVTKSTLKPNILLVEDNKINIMVTKSMMKQLGYSMDVVNNGVEAIRAVQSHSYDIILMDVYMPVMNGLQTTKLIRSYEETGNWDAAREAGVEQSISASDECSVPPKKRIHIVAMTANTMSESAEECFANGMDAFVSKPVTFLKLKECLEKYLR*