AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG000591

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG000591 MtrunA17_Chr1g0150281 96.491 285 9 1 1 284 1 285 0.0 570
MsaG000591 MtrunA17_Chr3g0136961 58.983 295 92 9 1 275 1 286 3.63e-102 300
MsaG000591 MtrunA17_Chr2g0333851 46.494 271 111 5 24 275 58 313 3.27e-67 211
MsaG000591 MtrunA17_Chr4g0001361 42.014 288 131 9 11 275 40 314 4.67e-52 172
MsaG000591 MtrunA17_Chr1g0150271 38.306 248 122 6 46 276 5 238 3.80e-42 145
MsaG000591 MtrunA17_Chr7g0270831 29.080 337 165 8 6 274 71 401 1.24e-31 121
MsaG000591 MtrunA17_Chr2g0288871 32.864 213 99 4 28 196 150 362 2.55e-27 110
MsaG000591 MtrunA17_Chr3g0099641 33.010 206 80 5 124 271 236 441 7.66e-25 103
MsaG000591 MtrunA17_Chr4g0075201 30.000 220 105 5 25 205 124 333 1.07e-24 103
MsaG000591 MtrunA17_Chr4g0051181 52.564 78 37 0 119 196 223 300 6.06e-24 100
MsaG000591 MtrunA17_Chr4g0060271 56.000 75 33 0 122 196 238 312 1.21e-23 100
MsaG000591 MtrunA17_Chr2g0322391 48.889 90 46 0 124 213 334 423 2.39e-23 99.4
MsaG000591 MtrunA17_Chr7g0216891 53.333 75 35 0 122 196 202 276 2.83e-23 99.4
MsaG000591 MtrunA17_Chr2g0302391 41.739 115 56 3 91 196 156 268 8.74e-23 97.1
MsaG000591 MtrunA17_Chr4g0025061 36.184 152 82 4 65 215 299 436 1.72e-20 91.3
MsaG000591 MtrunA17_Chr4g0025051 52.055 73 35 0 124 196 320 392 1.97e-20 90.9
MsaG000591 MtrunA17_Chr1g0194481 41.667 96 51 2 123 218 146 236 1.62e-19 86.7
MsaG000591 MtrunA17_Chr5g0445311 57.895 57 23 1 140 196 365 420 1.76e-19 88.2
MsaG000591 MtrunA17_Chr3g0101131 39.286 112 59 4 91 196 363 471 2.00e-19 88.2
MsaG000591 MtrunA17_Chr5g0401821 54.386 57 26 0 140 196 200 256 2.12e-19 85.5
MsaG000591 MtrunA17_Chr7g0270191 49.351 77 36 2 120 196 128 201 2.52e-19 85.9
MsaG000591 MtrunA17_Chr5g0447701 52.703 74 32 2 124 197 239 309 2.85e-19 86.7
MsaG000591 MtrunA17_Chr5g0443131 47.297 74 34 2 123 196 145 213 4.59e-19 85.1
MsaG000591 MtrunA17_Chr4g0051881 57.895 57 23 1 140 196 179 234 4.75e-19 85.5
MsaG000591 MtrunA17_Chr5g0418781 42.308 104 48 4 99 196 83 180 4.79e-19 84.7
MsaG000591 MtrunA17_Chr4g0053281 50.769 65 32 0 140 204 267 331 5.40e-19 85.5
MsaG000591 MtrunA17_Chr1g0184691 49.315 73 35 1 127 197 279 351 7.30e-19 85.5
MsaG000591 MtrunA17_Chr1g0147881 36.538 104 55 1 93 196 227 319 1.47e-18 84.3
MsaG000591 MtrunA17_Chr5g0432161 41.935 93 47 4 105 196 391 477 3.98e-18 84.3
MsaG000591 MtrunA17_Chr5g0432161 50.877 57 26 2 140 196 245 299 1.38e-13 70.9
MsaG000591 MtrunA17_Chr7g0263531 50.746 67 32 1 130 196 352 417 4.35e-18 83.6
MsaG000591 MtrunA17_Chr7g0263531 53.333 75 26 4 140 207 190 262 1.44e-16 79.3
MsaG000591 MtrunA17_Chr2g0294231 47.297 74 34 2 123 196 191 259 4.83e-18 83.2
MsaG000591 MtrunA17_Chr4g0042831 46.341 82 38 3 115 196 259 334 4.89e-18 83.2
MsaG000591 MtrunA17_Chr4g0042831 47.368 57 29 1 140 196 146 201 8.06e-11 62.0
MsaG000591 MtrunA17_Chr5g0418191 45.122 82 39 3 115 196 301 376 5.02e-18 83.6
MsaG000591 MtrunA17_Chr5g0418191 54.386 57 24 2 140 196 152 206 3.77e-14 72.0
MsaG000591 MtrunA17_Chr7g0228941 52.632 57 27 0 140 196 53 109 7.08e-18 77.8
MsaG000591 MtrunA17_Chr7g0218081 34.127 126 77 3 71 196 347 466 1.08e-17 82.8
MsaG000591 MtrunA17_Chr7g0218081 49.123 57 27 2 140 196 229 283 2.08e-11 63.9
MsaG000591 MtrunA17_Chr1g0188941 50.877 57 27 1 140 196 52 107 1.26e-17 77.0
MsaG000591 MtrunA17_Chr7g0238921 49.333 75 37 1 140 214 125 198 3.13e-17 80.1
MsaG000591 MtrunA17_Chr3g0106491 46.575 73 36 2 124 196 352 421 5.34e-17 80.9
MsaG000591 MtrunA17_Chr3g0106491 52.632 57 25 2 140 196 199 253 9.51e-14 71.2
MsaG000591 MtrunA17_Chr8g0379461 51.724 58 28 0 140 197 77 134 7.17e-17 78.2
MsaG000591 MtrunA17_Chr1g0150801 50.877 57 27 1 140 196 103 158 1.25e-16 75.5
MsaG000591 MtrunA17_Chr4g0004911 41.304 92 48 3 105 196 307 392 1.31e-16 79.7
MsaG000591 MtrunA17_Chr4g0004911 49.123 57 28 1 140 196 185 240 2.19e-13 70.1
MsaG000591 MtrunA17_Chr7g0249301 42.857 84 42 3 113 196 271 348 1.88e-16 78.6
MsaG000591 MtrunA17_Chr4g0071521 50.000 58 29 0 140 197 246 303 2.23e-16 77.8
MsaG000591 MtrunA17_Chr3g0139021 52.632 57 27 0 140 196 315 371 2.43e-16 78.6
MsaG000591 MtrunA17_Chr2g0284321 49.123 57 29 0 140 196 36 92 2.61e-16 75.9
MsaG000591 MtrunA17_Chr3g0079691 34.921 126 70 4 124 240 482 604 3.57e-16 78.6
MsaG000591 MtrunA17_Chr3g0079691 50.877 57 26 2 140 196 281 335 4.91e-12 66.2
MsaG000591 MtrunA17_Chr7g0224801 52.459 61 28 1 140 200 94 153 4.16e-16 74.3
MsaG000591 MtrunA17_Chr3g0129361 52.632 57 26 1 140 196 145 200 5.15e-16 75.1
MsaG000591 MtrunA17_Chr8g0383351 52.632 57 26 1 140 196 161 216 5.47e-16 75.5
MsaG000591 MtrunA17_Chr2g0289181 33.607 122 74 3 140 254 15 136 9.05e-16 74.7
MsaG000591 MtrunA17_Chr7g0243891 45.205 73 37 2 124 196 361 430 1.05e-15 77.0
MsaG000591 MtrunA17_Chr7g0243891 49.123 57 27 2 140 196 217 271 2.03e-11 63.9
MsaG000591 MtrunA17_Chr4g0068541 45.205 73 37 2 124 196 195 264 1.34e-15 76.3
MsaG000591 MtrunA17_Chr7g0249311 32.911 158 88 7 113 262 371 518 1.80e-15 76.3
MsaG000591 MtrunA17_Chr8g0335811 52.632 57 26 1 140 196 155 210 1.92e-15 73.9
MsaG000591 MtrunA17_Chr2g0319961 52.632 57 27 0 140 196 81 137 1.93e-15 74.3
MsaG000591 MtrunA17_Chr2g0293481 42.857 77 39 1 125 196 248 324 1.11e-14 73.9
MsaG000591 MtrunA17_Chr4g0074001 49.123 57 28 1 140 196 360 415 1.42e-14 73.6
MsaG000591 MtrunA17_Chr8g0386241 45.161 62 33 1 140 201 104 164 1.48e-14 70.5
MsaG000591 MtrunA17_Chr8g0387501 49.123 57 29 0 140 196 130 186 2.05e-14 71.6
MsaG000591 MtrunA17_Chr3g0129321 49.123 57 28 1 140 196 119 174 2.55e-14 70.1
MsaG000591 MtrunA17_Chr8g0348311 45.614 57 31 0 140 196 156 212 6.17e-14 70.9
MsaG000591 MtrunA17_Chr4g0024391 35.870 92 59 0 124 215 214 305 8.06e-14 71.2
MsaG000591 MtrunA17_Chr8g0383251 31.343 134 77 5 66 196 43 164 8.08e-14 68.6
MsaG000591 MtrunA17_Chr7g0248721 52.459 61 27 2 140 200 142 200 1.02e-13 68.9
MsaG000591 MtrunA17_Chr7g0270051 47.368 57 30 0 140 196 54 110 1.04e-13 69.7
MsaG000591 MtrunA17_Chr7g0248691 47.368 57 30 0 140 196 155 211 1.31e-13 70.1
MsaG000591 MtrunA17_Chr6g0485431 39.773 88 47 4 111 197 130 212 1.32e-13 70.1
MsaG000591 MtrunA17_Chr1g0202461 36.842 76 48 0 140 215 262 337 6.48e-13 68.2
MsaG000591 MtrunA17_Chr1g0189391 37.234 94 53 4 105 197 67 155 8.47e-13 67.8
MsaG000591 MtrunA17_Chr8g0387981 50.877 57 26 1 140 194 133 189 5.24e-12 65.5
MsaG000591 MtrunA17_Chr5g0447711 46.479 71 36 2 126 196 218 286 7.55e-12 64.7
MsaG000591 MtrunA17_Chr3g0126391 50.877 57 26 1 140 194 134 190 8.89e-12 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG000591 AT1G80840.1 42.140 299 136 8 3 274 6 294 5.22e-69 216
MsaG000591 AT2G25000.1 41.697 271 110 8 19 278 36 269 1.42e-59 191
MsaG000591 AT4G31800.1 43.657 268 129 8 25 278 49 308 1.46e-59 192
MsaG000591 AT2G25000.2 41.825 263 108 7 24 278 1 226 2.37e-59 189
MsaG000591 AT2G25000.4 41.825 263 108 7 24 278 1 226 2.37e-59 189
MsaG000591 AT2G25000.3 41.729 266 110 7 21 278 20 248 5.43e-59 189
MsaG000591 AT4G31800.2 54.706 170 67 4 111 278 82 243 4.20e-53 173
MsaG000591 AT4G31800.3 41.765 170 76 4 25 176 49 213 2.20e-32 119
MsaG000591 AT4G01720.1 33.503 197 98 4 28 196 105 296 1.82e-24 102
MsaG000591 AT4G04450.2 48.913 92 47 0 124 215 183 274 2.08e-24 102
MsaG000591 AT4G04450.1 48.913 92 47 0 124 215 277 368 5.01e-24 101
MsaG000591 AT1G68150.1 49.485 97 48 1 115 210 210 306 6.96e-24 100
MsaG000591 AT4G22070.4 54.795 73 33 0 124 196 282 354 5.37e-23 98.6
MsaG000591 AT4G22070.3 54.795 73 33 0 124 196 282 354 5.37e-23 98.6
MsaG000591 AT4G22070.2 54.795 73 33 0 124 196 282 354 5.37e-23 98.6
MsaG000591 AT4G22070.1 54.795 73 33 0 124 196 282 354 5.37e-23 98.6
MsaG000591 AT1G62300.1 54.795 73 33 0 124 196 297 369 8.18e-23 98.2
MsaG000591 AT5G15130.2 52.703 74 35 0 140 213 186 259 4.76e-22 95.9
MsaG000591 AT5G15130.1 52.703 74 35 0 140 213 228 301 6.33e-22 95.5
MsaG000591 AT1G18860.1 64.912 57 20 0 140 196 240 296 2.02e-21 94.0
MsaG000591 AT1G29860.1 53.333 60 27 1 137 196 134 192 4.56e-19 85.1
MsaG000591 AT4G18170.1 53.333 60 27 1 137 196 170 228 4.64e-19 85.9
MsaG000591 AT1G64000.1 57.377 61 25 1 140 200 115 174 5.07e-19 83.2
MsaG000591 AT2G47260.1 55.000 60 26 1 137 196 172 230 1.28e-18 84.7
MsaG000591 AT2G04880.2 57.627 59 24 1 138 196 282 339 1.30e-18 85.9
MsaG000591 AT5G26170.1 50.877 57 27 1 140 196 114 169 1.33e-18 81.6
MsaG000591 AT5G07100.2 38.614 101 58 3 96 196 101 197 1.39e-18 82.4
MsaG000591 AT5G07100.2 46.479 71 37 1 126 196 11 80 6.94e-14 69.7
MsaG000591 AT5G07100.4 38.614 101 58 3 96 196 101 197 1.39e-18 82.4
MsaG000591 AT5G07100.4 46.479 71 37 1 126 196 11 80 6.94e-14 69.7
MsaG000591 AT5G07100.5 38.614 101 58 3 96 196 101 197 1.39e-18 82.4
MsaG000591 AT5G07100.5 46.479 71 37 1 126 196 11 80 6.94e-14 69.7
MsaG000591 AT5G07100.3 38.614 101 58 3 96 196 101 197 1.39e-18 82.4
MsaG000591 AT5G07100.3 46.479 71 37 1 126 196 11 80 6.94e-14 69.7
MsaG000591 AT2G04880.1 57.627 59 24 1 138 196 306 363 1.46e-18 85.5
MsaG000591 AT5G41570.1 57.377 61 25 1 140 200 99 158 2.30e-18 81.3
MsaG000591 AT4G26640.5 56.140 57 24 1 140 196 206 261 2.73e-18 84.3
MsaG000591 AT4G26640.6 56.140 57 24 1 140 196 206 261 2.73e-18 84.3
MsaG000591 AT4G26640.4 56.140 57 24 1 140 196 206 261 2.73e-18 84.3
MsaG000591 AT4G26640.3 56.140 57 24 1 140 196 206 261 2.73e-18 84.3
MsaG000591 AT1G13960.2 45.122 82 39 3 115 196 237 312 3.31e-18 84.0
MsaG000591 AT1G13960.2 52.632 57 25 2 140 196 77 131 4.61e-13 68.9
MsaG000591 AT4G26640.1 56.140 57 24 1 140 196 310 365 4.35e-18 84.3
MsaG000591 AT4G26640.7 56.140 57 24 1 140 196 342 397 4.39e-18 84.3
MsaG000591 AT4G26640.2 56.140 57 24 1 140 196 382 437 4.56e-18 84.3
MsaG000591 AT5G46350.1 47.222 72 33 2 125 196 173 239 5.86e-18 82.8
MsaG000591 AT4G31550.1 52.632 57 27 0 140 196 247 303 5.92e-18 82.8
MsaG000591 AT4G31550.2 52.632 57 27 0 140 196 246 302 6.09e-18 82.8
MsaG000591 AT5G49520.1 47.222 72 34 2 140 208 222 292 6.18e-18 83.6
MsaG000591 AT2G03340.1 45.122 82 39 3 115 196 396 471 6.72e-18 84.0
MsaG000591 AT2G03340.1 52.632 57 25 2 140 196 251 305 9.81e-14 71.6
MsaG000591 AT1G13960.1 45.122 82 39 3 115 196 390 465 7.19e-18 83.6
MsaG000591 AT1G13960.1 52.632 57 25 2 140 196 230 284 5.98e-13 68.9
MsaG000591 AT5G07100.1 38.614 101 58 3 96 196 194 290 7.24e-18 82.4
MsaG000591 AT5G07100.1 46.479 71 37 1 126 196 104 173 2.30e-13 69.3
MsaG000591 AT2G30590.1 54.386 57 26 0 140 196 314 370 7.49e-18 83.2
MsaG000591 AT1G69810.2 50.000 74 36 1 124 196 130 203 2.70e-17 80.9
MsaG000591 AT3G62340.1 51.562 64 30 1 133 196 112 174 3.17e-17 80.1
MsaG000591 AT1G69310.4 54.386 57 25 1 140 196 148 203 3.33e-17 80.1
MsaG000591 AT1G69310.2 54.386 57 25 1 140 196 148 203 3.33e-17 80.1
MsaG000591 AT1G69310.1 54.386 57 25 1 140 196 148 203 3.33e-17 80.1
MsaG000591 AT1G69810.1 50.000 74 36 1 124 196 188 261 3.48e-17 81.3
MsaG000591 AT2G23320.1 49.123 57 29 0 140 196 241 297 4.16e-17 80.1
MsaG000591 AT2G24570.1 52.632 57 27 0 140 196 244 300 4.87e-17 80.1
MsaG000591 AT5G56270.1 47.945 73 35 2 124 196 474 543 5.01e-17 81.3
MsaG000591 AT5G56270.1 27.461 193 121 6 9 196 150 328 6.18e-12 66.2
MsaG000591 AT5G13080.1 53.226 62 28 1 140 201 68 128 6.43e-17 76.3
MsaG000591 AT3G01080.1 43.902 82 40 3 115 196 287 362 7.89e-17 80.5
MsaG000591 AT3G01080.1 49.123 57 27 2 140 196 168 222 8.29e-11 62.4
MsaG000591 AT5G28650.1 45.833 72 37 1 127 196 248 319 8.47e-17 79.7
MsaG000591 AT1G29280.1 50.877 57 28 0 140 196 75 131 9.01e-17 78.6
MsaG000591 AT3G01080.2 46.575 73 36 2 124 196 331 400 9.40e-17 80.1
MsaG000591 AT3G01080.2 49.123 57 27 2 140 196 206 260 7.88e-11 62.8
MsaG000591 AT4G24240.1 48.276 58 30 0 140 197 282 339 1.42e-16 79.3
MsaG000591 AT3G04670.1 44.444 72 38 1 127 196 248 319 2.15e-16 78.6
MsaG000591 AT2G38470.1 52.632 57 26 1 140 196 363 418 3.11e-16 79.0
MsaG000591 AT2G38470.1 52.632 57 25 2 140 196 185 239 1.10e-13 71.2
MsaG000591 AT2G44745.1 52.632 57 26 1 140 196 146 201 1.31e-15 74.3
MsaG000591 AT3G01970.1 49.180 61 30 1 140 200 66 125 1.46e-15 72.8
MsaG000591 AT4G30935.1 45.455 66 35 1 131 196 323 387 1.64e-15 76.6
MsaG000591 AT2G30250.1 45.205 73 37 2 124 196 315 384 2.47e-15 75.9
MsaG000591 AT2G30250.1 46.479 71 34 3 140 210 167 233 2.36e-11 63.9
MsaG000591 AT2G37260.3 45.588 68 36 1 129 196 259 325 4.83e-15 74.7
MsaG000591 AT2G37260.3 45.070 71 37 2 126 196 72 140 1.29e-14 73.6
MsaG000591 AT2G37260.1 45.588 68 36 1 129 196 339 405 5.31e-15 75.1
MsaG000591 AT2G37260.1 45.070 71 37 2 126 196 152 220 1.33e-14 73.9
MsaG000591 AT2G37260.2 45.588 68 36 1 129 196 257 323 5.35e-15 74.7
MsaG000591 AT2G37260.2 45.070 71 37 2 126 196 70 138 1.36e-14 73.6
MsaG000591 AT1G55600.1 50.000 62 30 1 140 201 308 368 5.44e-15 75.1
MsaG000591 AT2G34830.1 47.692 65 34 0 132 196 208 272 7.13e-15 74.7
MsaG000591 AT2G34830.2 47.692 65 34 0 132 196 217 281 7.37e-15 74.7
MsaG000591 AT4G39410.1 50.877 57 27 1 140 196 224 279 9.57e-15 73.6
MsaG000591 AT2G21900.1 44.444 72 38 2 126 196 95 165 1.04e-14 71.6
MsaG000591 AT1G30650.1 47.692 65 34 0 132 196 210 274 1.58e-14 73.6
MsaG000591 AT3G58710.1 49.123 57 29 0 140 196 72 128 2.33e-14 72.0
MsaG000591 AT3G58710.2 49.123 57 29 0 140 196 71 127 2.66e-14 71.6
MsaG000591 AT5G64810.1 45.614 57 30 1 140 196 111 166 8.37e-14 68.9
MsaG000591 AT4G01250.1 45.614 57 31 0 140 196 129 185 8.94e-14 70.5
MsaG000591 AT2G46130.1 50.877 57 27 1 140 196 31 86 1.11e-13 66.6
MsaG000591 AT5G43290.1 40.789 76 43 2 121 196 97 170 1.87e-13 69.3
MsaG000591 AT5G52830.1 49.123 57 29 0 140 196 166 222 2.16e-13 69.7
MsaG000591 AT4G26440.1 43.836 73 38 2 124 196 359 428 2.67e-13 70.1
MsaG000591 AT4G26440.1 47.368 57 28 2 140 196 179 233 7.42e-11 62.8
MsaG000591 AT5G45050.2 34.951 103 64 1 94 196 1110 1209 4.52e-13 69.7
MsaG000591 AT5G45050.1 34.951 103 64 1 94 196 1138 1237 4.75e-13 69.7
MsaG000591 AT5G45050.4 34.951 103 64 1 94 196 999 1098 5.12e-13 69.7
MsaG000591 AT1G69310.3 50.877 57 23 2 140 196 148 199 9.00e-13 67.4
MsaG000591 AT4G23550.1 30.435 138 87 3 140 275 141 271 1.40e-12 67.4
MsaG000591 AT2G46400.1 43.836 73 37 2 124 194 91 161 4.09e-12 65.9
MsaG000591 AT3G01080.3 49.123 57 27 2 140 196 206 260 7.56e-11 62.4
MsaG000591 AT5G24110.1 27.778 162 99 5 50 194 10 170 9.68e-11 61.6

Find 45 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTTCACCATTTGTTCTTAT+TGG 0.165077 1:-7787427 None:intergenic
GCACTTGCCACTGCTATTTC+TGG 0.167473 1:+7787384 MsaT000591.1:CDS
AATTGCTGGATAGATGATTT+TGG 0.173303 1:-7787309 None:intergenic
TGGAACATTATCAAGCTTAA+TGG 0.220468 1:-7787289 None:intergenic
TTCCTCACCTAAGGCTTATA+AGG 0.237117 1:+7786196 MsaT000591.1:CDS
ATTTGTTCTTATTGGAAGTA+TGG 0.275960 1:-7787419 None:intergenic
TCTTTCTTGATTGTTGTGTT+TGG 0.315541 1:-7786046 None:intergenic
GCAAGTGCTGTAGTGAAATT+AGG 0.319456 1:-7787369 None:intergenic
CAAAGTGAAGAAGCTCCTTT+AGG 0.328438 1:+7787216 MsaT000591.1:CDS
GGATATCAATGGAGGAAATA+TGG 0.328462 1:+7786431 MsaT000591.1:CDS
AATATGTTTGATACTGTTAG+AGG 0.330331 1:+7786095 MsaT000591.1:CDS
TTATGTGCAGGACGAAGTTT+TGG 0.348061 1:+7785899 MsaT000591.1:intron
CCTCTCCTAGAGCTTACTTT+AGG 0.371319 1:+7786477 MsaT000591.1:CDS
TATGTTGCAACTAGTATAGT+AGG 0.404321 1:-7787138 None:intergenic
ATGAAGAATCATTAATCAAA+AGG 0.454669 1:+7786159 MsaT000591.1:CDS
CTAAAGGAGCTTCTTCACTT+TGG 0.478227 1:-7787215 None:intergenic
ATACTTCCAATAAGAACAAA+TGG 0.478829 1:+7787421 MsaT000591.1:CDS
ATGGAGGAAATATGGTCAAA+AGG 0.485930 1:+7786439 MsaT000591.1:CDS
ACAAGAACCTTATAAGCCTT+AGG 0.486641 1:-7786203 None:intergenic
ACATTATCAAGCTTAATGGT+TGG 0.490629 1:-7787285 None:intergenic
CATTAATCAAAAGGCCATGT+AGG 0.493040 1:+7786168 MsaT000591.1:CDS
ATACTAGTTGCAACATATGA+AGG 0.494693 1:+7787144 MsaT000591.1:CDS
TAGGGATATTTCCTCACCTA+AGG 0.500791 1:+7786187 MsaT000591.1:CDS
AACTGGGCAGCTTGGAGCAT+AGG 0.506437 1:-7786504 None:intergenic
TAGTGCTTCTTCTATACTCA+AGG 0.512134 1:+7785430 MsaT000591.1:CDS
GAGCACCTAAAGTAAGCTCT+AGG 0.538868 1:-7786482 None:intergenic
ATTAATCAAAAGGCCATGTA+GGG 0.538909 1:+7786169 MsaT000591.1:CDS
CCTAAAGTAAGCTCTAGGAG+AGG 0.548395 1:-7786477 None:intergenic
CTCATAGTTCTCACACATGT+TGG 0.554828 1:-7785976 None:intergenic
GTAACATGAACTGAACCTAA+AGG 0.560432 1:-7787231 None:intergenic
CTAAAGTAAGCTCTAGGAGA+GGG 0.569732 1:-7786476 None:intergenic
GTCTCAGTTCTGAGAACAAG+AGG 0.575865 1:+7785937 MsaT000591.1:CDS
TTTCAGCTTTCTCATGACCA+TGG 0.582497 1:-7787176 None:intergenic
AACAATGGAATCAACATGCG+TGG 0.589289 1:+7785385 None:intergenic
GGTATAAAGTATGTGATGGA+TGG 0.592987 1:+7786410 MsaT000591.1:intron
ATGTGATGGATGGATATCAA+TGG 0.593080 1:+7786420 MsaT000591.1:CDS
TTGTGGTATAAAGTATGTGA+TGG 0.595565 1:+7786406 MsaT000591.1:intron
ATGTTGCAACTAGTATAGTA+GGG 0.601976 1:-7787137 None:intergenic
GGTGAGGAAATATCCCTACA+TGG 0.620508 1:-7786182 None:intergenic
GTTCTTTGAATAATTTGTTG+AGG 0.631715 1:-7787258 None:intergenic
AACCTTATAAGCCTTAGGTG+AGG 0.658225 1:-7786198 None:intergenic
TAAAGTAAGCTCTAGGAGAG+GGG 0.664718 1:-7786475 None:intergenic
TGATGGATGGATATCAATGG+AGG 0.669535 1:+7786423 MsaT000591.1:CDS
AATTCTTCCAGAAATAGCAG+TGG 0.681600 1:-7787391 None:intergenic
TATGAAGGAGAGCACAACCA+TGG 0.694590 1:+7787159 MsaT000591.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr1 gene 7785389 7787446 7785389 ID=MsaG000591
Chr1 mRNA 7785389 7787446 7785389 ID=MsaT000591.1;Parent=MsaG000591
Chr1 exon 7785389 7785451 7785389 ID=MsaT000591.1.exon1;Parent=MsaT000591.1
Chr1 CDS 7785389 7785451 7785389 ID=cds.MsaT000591.1;Parent=MsaT000591.1
Chr1 exon 7785909 7786253 7785909 ID=MsaT000591.1.exon2;Parent=MsaT000591.1
Chr1 CDS 7785909 7786253 7785909 ID=cds.MsaT000591.1;Parent=MsaT000591.1
Chr1 exon 7786419 7786535 7786419 ID=MsaT000591.1.exon3;Parent=MsaT000591.1
Chr1 CDS 7786419 7786535 7786419 ID=cds.MsaT000591.1;Parent=MsaT000591.1
Chr1 exon 7787117 7787446 7787117 ID=MsaT000591.1.exon4;Parent=MsaT000591.1
Chr1 CDS 7787117 7787446 7787117 ID=cds.MsaT000591.1;Parent=MsaT000591.1
Gene Sequence

>MsaT000591.1

ATGGAATCAACATGCGTGGATACTTCACTCAATCTTAACGTTAGTGCTTCTTCTATACTCAAGGACGAAGTTTTGGTTGAAGAGTTACATCGTCTCAGTTCTGAGAACAAGAGGCTAAATGAAACATTGACCAACATGTGTGAGAACTATGAGACTATGCAAAAGCAATTGAATCAATTGATGAATCAAAATTTTGAACACCAAACACAACAATCAAGAAAGAGAAAAGCTGAGAGTGAAAGTTGCATCAATATGTTTGATACTGTTAGAGGCATTAATATTATCAATAATAATGAGTGCAGCACTGTTACTGATGAAGAATCATTAATCAAAAGGCCATGTAGGGATATTTCCTCACCTAAGGCTTATAAGGTTCTTGTGAAGACAGAAGCATCTAGTAATAGCTTATATGTGATGGATGGATATCAATGGAGGAAATATGGTCAAAAGGTTACTAGAGATAACCCCTCTCCTAGAGCTTACTTTAGGTGCTCCTATGCTCCAAGCTGCCCAGTTAAAAAGAAGGTGCAAAAAAGTTTAGAAGACCCTACTATACTAGTTGCAACATATGAAGGAGAGCACAACCATGGTCATGAGAAAGCTGAAATATCAATGATATCAAGCCAAAGTGAAGAAGCTCCTTTAGGTTCAGTTCATGTTACTTCACCTCAACAAATTATTCAAAGAACATGTCCAACCATTAAGCTTGATAATGTTCCAAAATCATCTATCCAGCAATTTTTGGTTCAACAAATGGCTACTTCTTTGACAAATGATCCTAATTTCACTACAGCACTTGCCACTGCTATTTCTGGAAGAATTTTAGACCATACTTCCAATAAGAACAAATGGTGA
Protein sequence

>MsaT000591.1

MESTCVDTSLNLNVSASSILKDEVLVEELHRLSSENKRLNETLTNMCENYETMQKQLNQLMNQNFEHQTQQSRKRKAESESCINMFDTVRGINIINNNECSTVTDEESLIKRPCRDISSPKAYKVLVKTEASSNSLYVMDGYQWRKYGQKVTRDNPSPRAYFRCSYAPSCPVKKKVQKSLEDPTILVATYEGEHNHGHEKAEISMISSQSEEAPLGSVHVTSPQQIIQRTCPTIKLDNVPKSSIQQFLVQQMATSLTNDPNFTTALATAISGRILDHTSNKNKW*