AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG000625

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG000625 MtrunA17_Chr3g0137091 64.352 648 172 9 1 594 1 643 0.0 797
MsaG000625 MtrunA17_Chr1g0149871 89.873 316 32 0 50 365 8 323 0.0 598
MsaG000625 MtrunA17_Chr5g0442141 40.806 397 202 10 132 509 455 837 3.11e-84 289
MsaG000625 MtrunA17_Chr3g0095091 38.791 397 210 10 132 509 409 791 4.07e-80 277
MsaG000625 MtrunA17_Chr4g0052341 42.010 388 199 11 129 509 600 968 5.40e-78 271
MsaG000625 MtrunA17_Chr5g0403921 40.283 283 138 7 249 513 148 417 9.09e-49 182
MsaG000625 MtrunA17_Chr1g0205891 38.321 274 132 6 259 509 341 600 8.14e-46 175
MsaG000625 MtrunA17_Chr5g0394201 39.033 269 127 6 264 509 333 587 3.10e-45 173
MsaG000625 MtrunA17_Chr4g0066741 40.000 235 122 3 284 513 186 406 1.05e-43 168
MsaG000625 MtrunA17_Chr7g0257101 39.004 241 127 5 281 509 573 805 1.32e-42 165
MsaG000625 MtrunA17_Chr2g0286291 39.574 235 123 3 284 513 189 409 1.59e-42 165
MsaG000625 MtrunA17_Chr7g0232121 38.934 244 130 5 284 513 37 275 7.16e-41 159
MsaG000625 MtrunA17_Chr4g0066651 39.062 256 115 7 278 509 981 1219 1.35e-40 159
MsaG000625 MtrunA17_Chr1g0175191 39.918 243 118 5 280 509 515 742 1.46e-40 159
MsaG000625 MtrunA17_Chr7g0231521 41.509 212 115 4 284 491 37 243 6.48e-40 156
MsaG000625 MtrunA17_Chr7g0248031 32.047 337 173 6 278 576 986 1304 1.03e-37 150
MsaG000625 MtrunA17_Chr8g0348721 30.564 337 177 7 278 576 979 1296 1.46e-35 144
MsaG000625 MtrunA17_Chr1g0163011 35.573 253 139 7 286 525 195 436 7.17e-35 140
MsaG000625 MtrunA17_Chr4g0017211 33.803 213 114 3 285 487 1449 1644 6.55e-30 126
MsaG000625 MtrunA17_Chr1g0183681 28.723 282 160 8 244 487 336 614 6.87e-27 117
MsaG000625 MtrunA17_Chr7g0264371 26.419 458 253 15 54 475 49 458 1.80e-25 112
MsaG000625 MtrunA17_Chr8g0384531 34.842 221 106 7 286 488 384 584 1.30e-22 103
MsaG000625 MtrunA17_Chr5g0393461 31.019 216 125 5 286 488 184 388 1.06e-21 100
MsaG000625 MtrunA17_Chr4g0040301 34.054 185 91 5 314 485 18 184 2.76e-21 98.6
MsaG000625 MtrunA17_Chr2g0323241 51.546 97 38 2 527 623 100 187 6.51e-21 96.3
MsaG000625 MtrunA17_Chr6g0457751 79.592 49 7 1 324 372 7 52 3.63e-20 84.7
MsaG000625 MtrunA17_Chr1g0190391 28.821 229 119 6 286 484 582 796 1.50e-16 83.6
MsaG000625 MtrunA17_Chr4g0023111 50.667 75 36 1 559 632 145 219 5.93e-16 82.0
MsaG000625 MtrunA17_Chr1g0168031 29.000 200 89 8 308 472 536 717 4.76e-13 72.8
MsaG000625 MtrunA17_Chr7g0255821 24.138 232 133 7 308 514 252 465 2.01e-11 67.4
MsaG000625 MtrunA17_Chr2g0320751 24.151 265 120 7 308 509 400 646 2.55e-11 67.0
MsaG000625 MtrunA17_Chr4g0069451 26.923 208 132 8 286 487 174 367 5.21e-11 65.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG000625 AT2G25170.1 58.540 644 200 10 1 594 1 627 0.0 707
MsaG000625 AT2G25170.4 58.540 644 200 10 1 594 1 627 0.0 707
MsaG000625 AT2G25170.2 58.540 644 200 10 1 594 18 644 0.0 707
MsaG000625 AT2G25170.3 58.540 644 200 10 1 594 27 653 0.0 706
MsaG000625 AT4G31900.3 54.644 463 163 9 98 538 43 480 1.19e-153 474
MsaG000625 AT4G31900.1 54.644 463 163 9 98 538 43 480 1.79e-153 474
MsaG000625 AT4G31900.2 57.105 373 118 6 183 538 92 439 1.16e-128 407
MsaG000625 AT2G13370.1 39.798 397 206 10 132 509 463 845 7.05e-82 283
MsaG000625 AT2G13370.2 39.798 397 206 10 132 509 463 845 7.05e-82 283
MsaG000625 AT2G13370.3 39.798 397 206 10 132 509 463 845 7.05e-82 283
MsaG000625 AT5G44800.1 38.053 452 233 14 113 552 527 943 2.05e-71 252
MsaG000625 AT5G19310.2 41.434 251 117 5 278 513 114 349 5.69e-46 174
MsaG000625 AT5G19310.1 41.434 251 117 5 278 513 378 613 1.25e-45 174
MsaG000625 AT5G66750.1 38.542 288 139 9 243 509 160 430 3.86e-45 172
MsaG000625 AT3G06010.1 38.462 273 131 6 260 509 369 627 3.28e-44 170
MsaG000625 AT5G18620.2 40.851 235 120 3 284 513 192 412 1.36e-43 168
MsaG000625 AT5G18620.1 40.851 235 120 3 284 513 192 412 1.42e-43 168
MsaG000625 AT3G06400.3 40.000 235 122 3 284 513 187 407 5.54e-43 167
MsaG000625 AT3G06400.1 40.000 235 122 3 284 513 187 407 5.68e-43 166
MsaG000625 AT3G06400.2 40.000 235 122 3 284 513 187 407 5.74e-43 166
MsaG000625 AT3G57300.3 39.496 238 124 6 284 509 584 813 1.08e-40 160
MsaG000625 AT3G57300.1 39.496 238 124 6 284 509 584 813 1.17e-40 160
MsaG000625 AT3G57300.4 39.496 238 124 6 284 509 584 813 1.17e-40 160
MsaG000625 AT2G28290.2 33.956 321 150 7 234 509 681 984 1.26e-39 157
MsaG000625 AT2G28290.6 33.956 321 150 7 234 509 682 985 1.31e-39 157
MsaG000625 AT2G28290.5 33.956 321 150 7 234 509 682 985 1.33e-39 157
MsaG000625 AT2G28290.3 33.956 321 150 7 234 509 681 984 1.34e-39 157
MsaG000625 AT2G28290.4 33.956 321 150 7 234 509 681 984 1.34e-39 157
MsaG000625 AT2G28290.1 33.956 321 150 7 234 509 681 984 1.34e-39 157
MsaG000625 AT3G12810.2 39.506 243 119 5 280 509 526 753 2.24e-39 156
MsaG000625 AT3G12810.1 39.506 243 119 5 280 509 530 757 2.29e-39 156
MsaG000625 AT2G46020.2 30.749 374 201 7 241 576 938 1291 1.25e-38 154
MsaG000625 AT2G46020.3 30.749 374 201 7 241 576 938 1291 1.25e-38 154
MsaG000625 AT2G46020.4 30.749 374 201 7 241 576 938 1291 1.25e-38 154
MsaG000625 AT2G46020.5 30.667 375 199 8 241 576 938 1290 1.39e-37 150
MsaG000625 AT2G46020.1 30.667 375 199 8 241 576 938 1290 1.39e-37 150
MsaG000625 AT2G46020.6 30.667 375 199 8 241 576 938 1290 1.39e-37 150
MsaG000625 AT2G44980.3 38.306 248 135 6 284 518 49 291 2.23e-37 149
MsaG000625 AT3G57300.2 35.185 270 124 7 284 509 584 846 2.13e-36 147
MsaG000625 AT2G44980.2 37.751 249 136 7 284 518 49 292 3.43e-35 142
MsaG000625 AT2G02090.1 36.929 241 128 7 286 513 213 442 6.74e-35 141
MsaG000625 AT2G44980.1 36.585 246 134 7 284 518 49 283 3.20e-33 136
MsaG000625 AT3G54280.1 31.034 290 163 8 216 487 1375 1645 2.54e-31 131
MsaG000625 AT3G54280.2 31.034 290 163 8 216 487 1406 1676 3.15e-31 131
MsaG000625 AT1G03750.1 33.778 225 113 4 276 485 130 333 9.75e-29 122
MsaG000625 AT2G18760.4 33.036 224 126 6 286 487 385 606 1.37e-25 113
MsaG000625 AT2G18760.1 33.036 224 126 6 286 487 385 606 1.37e-25 113
MsaG000625 AT2G18760.2 33.036 224 126 6 286 487 385 606 1.37e-25 113
MsaG000625 AT2G18760.7 33.036 224 126 6 286 487 385 606 1.37e-25 113
MsaG000625 AT2G18760.5 33.036 224 126 6 286 487 385 606 1.37e-25 113
MsaG000625 AT2G18760.6 33.036 224 126 6 286 487 385 606 1.37e-25 113
MsaG000625 AT2G18760.3 33.036 224 126 6 286 487 444 665 1.58e-25 113
MsaG000625 AT5G63950.1 32.677 254 127 9 278 507 370 603 4.38e-24 108
MsaG000625 AT5G63950.2 33.058 242 125 10 278 507 370 586 6.85e-22 101
MsaG000625 AT3G19210.3 29.358 218 126 6 286 488 180 384 6.41e-20 94.7
MsaG000625 AT3G19210.2 29.358 218 126 6 286 488 180 384 7.30e-20 94.7
MsaG000625 AT3G19210.1 29.358 218 126 6 286 488 180 384 9.08e-20 94.7
MsaG000625 AT1G08600.1 31.795 195 108 6 300 489 743 917 1.45e-17 87.8
MsaG000625 AT1G02670.5 28.112 249 143 6 247 477 58 288 3.51e-17 85.9
MsaG000625 AT1G02670.6 28.112 249 143 6 247 477 58 288 3.85e-17 85.9
MsaG000625 AT1G02670.4 29.947 187 97 4 308 477 146 315 4.06e-17 85.5
MsaG000625 AT1G02670.3 28.112 249 143 6 247 477 85 315 4.94e-17 85.5
MsaG000625 AT1G02670.2 28.112 249 143 6 247 477 85 315 5.28e-17 85.5
MsaG000625 AT1G02670.1 29.834 181 93 4 308 471 157 320 4.47e-16 82.4
MsaG000625 AT1G02670.7 29.834 181 93 4 308 471 146 309 6.02e-16 82.0
MsaG000625 AT3G16600.1 27.083 240 142 9 278 488 46 281 1.39e-14 77.8
MsaG000625 AT4G03230.8 50.000 68 33 1 557 623 124 191 1.96e-14 77.4
MsaG000625 AT4G03230.5 50.000 68 33 1 557 623 52 119 2.32e-14 77.4
MsaG000625 AT4G03230.4 50.000 68 33 1 557 623 124 191 2.47e-14 77.4
MsaG000625 AT4G03230.1 50.000 68 33 1 557 623 124 191 2.47e-14 77.4
MsaG000625 AT2G21450.2 29.167 192 130 4 286 472 272 462 7.57e-14 75.5
MsaG000625 AT2G21450.1 29.167 192 130 4 286 472 265 455 8.28e-14 75.5
MsaG000625 AT5G07810.1 29.064 203 110 7 285 478 203 380 8.69e-14 75.5
MsaG000625 AT2G21450.3 29.167 192 130 4 286 472 174 364 9.45e-14 75.1
MsaG000625 AT1G48310.4 27.982 218 132 8 274 483 161 361 5.33e-13 72.4
MsaG000625 AT5G22750.1 25.094 267 122 9 308 514 416 664 6.10e-13 72.8
MsaG000625 AT1G48310.2 27.982 218 132 8 274 483 166 366 6.36e-13 72.4
MsaG000625 AT1G48310.1 27.982 218 132 8 274 483 161 361 6.78e-13 72.4
MsaG000625 AT1G48310.3 27.982 218 132 8 274 483 161 361 8.58e-13 72.0
MsaG000625 AT2G16390.2 28.934 197 128 6 283 472 350 541 1.06e-12 72.0
MsaG000625 AT2G16390.1 28.934 197 128 6 283 472 347 538 1.26e-12 71.6

Find 115 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGAAAGTGGATCCGAAAATT+TGG 0.164054 1:-8238257 MsaT000625.1:CDS
CATAGCCCCGGATTTCTTTC+AGG 0.189754 1:-8237080 MsaT000625.1:CDS
TGATGTCCTTAAACTCTTCT+TGG 0.228699 1:+8235197 None:intergenic
GATTCAATATATTTCGTTCT+AGG 0.231194 1:-8237193 MsaT000625.1:CDS
CACTTTCTACACTACAAAAC+TGG 0.252886 1:-8236666 MsaT000625.1:CDS
AGTATTGGACTTACACATTC+AGG 0.261513 1:+8238408 None:intergenic
AAGGTGAAGCTTCCAGTTCC+TGG 0.262756 1:+8215534 None:intergenic
AGAGTTCCAAGAAGAGTTTA+AGG 0.282522 1:-8235203 MsaT000625.1:CDS
AAGCTTCACCTTCCAACATT+TGG 0.291415 1:-8215523 MsaT000625.1:CDS
AGCTTCACCTTCCAACATTT+GGG 0.293646 1:-8215522 MsaT000625.1:CDS
CGATGAACCGTAGTCCATTC+AGG 0.311680 1:+8237862 None:intergenic
GAAAGTGGATCCGAAAATTT+GGG 0.323877 1:-8238256 MsaT000625.1:CDS
ACTCTGAAGCTCCTAGATTC+TGG 0.332248 1:-8215690 MsaT000625.1:CDS
TTCAATCCCTCAAGTTGATA+TGG 0.340274 1:+8236942 None:intergenic
TGTTATACTTGCTGATGAAA+TGG 0.341387 1:-8236886 MsaT000625.1:CDS
TTGTTTCGTCAAACATTGTT+TGG 0.346817 1:+8238231 None:intergenic
TAAGAAAGCAATACTTTGTA+TGG 0.354240 1:+8236735 None:intergenic
ATTGACAGGAACTCCTCTTC+AGG 0.369519 1:-8236109 MsaT000625.1:intron
TTCATCGAATACTTGCTTGC+AGG 0.388267 1:-8237846 MsaT000625.1:intron
AAAGCTCCCGTCTTGACAAC+TGG 0.401938 1:-8238437 MsaT000625.1:CDS
AAAATATGGCATTGATAACT+TGG 0.408671 1:-8215572 MsaT000625.1:CDS
TTAGAATTCTTCGGATTCTT+CGG 0.409217 1:+8236470 None:intergenic
GGACATCAATCAAGAGCAAC+AGG 0.410466 1:-8235182 MsaT000625.1:CDS
CCCACTGTTGCCGGCGAAAG+TGG 0.413833 1:-8238271 MsaT000625.1:CDS
AAGATGCGTCCCACTGTTGC+CGG 0.415465 1:-8238280 MsaT000625.1:CDS
TTTCTGTAGGTTATGTATGT+TGG 0.417971 1:-8236539 MsaT000625.1:intron
CTTATGCACTTCCTTGATGC+TGG 0.428463 1:-8235347 MsaT000625.1:CDS
AACGAGAATGACCCAGGAAC+TGG 0.433050 1:-8215546 MsaT000625.1:CDS
AAATGAGTAGCTTGGTTGAC+AGG 0.433236 1:-8239357 MsaT000625.1:CDS
TGATGCATTTCAGAACAATC+TGG 0.438877 1:-8235384 MsaT000625.1:intron
AATGGCACAAGTCAGAAAAC+TGG 0.442235 1:-8237908 MsaT000625.1:CDS
CTCTGAAGCTCCTAGATTCT+GGG 0.442968 1:-8215689 MsaT000625.1:CDS
TGAACTTCCTACGTTTCTCT+TGG 0.443048 1:-8236923 MsaT000625.1:CDS
GCACTAGATTCCCAGAATCT+AGG 0.446610 1:+8215679 None:intergenic
ACGATGAAAGTTGTTTACTT+TGG 0.457846 1:+8237933 None:intergenic
CATCTAGTAGTCTAAAAGAA+TGG 0.462724 1:-8231525 MsaT000625.1:CDS
AGAGAATGTTTCTCCACATC+TGG 0.463887 1:-8236698 MsaT000625.1:CDS
CTCTAATAGTGCTACGAGCT+TGG 0.468401 1:+8236510 None:intergenic
TCCCAATGGCATTCATCATA+AGG 0.469105 1:+8237257 None:intergenic
GCCTTCAGAAACTCTTCCTC+TGG 0.471383 1:+8237982 None:intergenic
TTCGAGGCTTCATACATTGT+TGG 0.477515 1:-8235158 MsaT000625.1:CDS
GTTATACTTGCTGATGAAAT+GGG 0.478199 1:-8236885 MsaT000625.1:CDS
TCCTTATGATGAATGCCATT+GGG 0.482192 1:-8237258 MsaT000625.1:CDS
AAACAACTTTCATCGTCAAA+TGG 0.484760 1:-8237926 MsaT000625.1:CDS
GCTTCAATGATGCGGCATCT+TGG 0.486161 1:+8236387 None:intergenic
CTACATCCATATCAACTTGA+GGG 0.493022 1:-8236948 MsaT000625.1:CDS
TTCCTTATGATGAATGCCAT+TGG 0.495300 1:-8237259 MsaT000625.1:CDS
ATCAAGAGCAACAGGTTTCG+AGG 0.495690 1:-8235174 MsaT000625.1:CDS
ATCTTATCAACATCAATAAG+TGG 0.496495 1:+8238313 None:intergenic
GATAAAGATGTATACTTCGC+AGG 0.496598 1:+8235125 None:intergenic
ATAGCCCCGGATTTCTTTCA+GGG 0.505132 1:-8237079 MsaT000625.1:CDS
TCGTTGCCATTCGACCTGAA+TGG 0.505730 1:-8237876 MsaT000625.1:CDS
GCACTTCCTTGATGCTGGGA+AGG 0.511269 1:-8235342 MsaT000625.1:intron
CAGGGACAATGGTGCTGAGA+AGG 0.514116 1:-8237306 MsaT000625.1:CDS
TCAGCAGAAAATGAGTAGCT+TGG 0.524164 1:-8239365 None:intergenic
CGAAACAATATCTTGTGAAG+TGG 0.524651 1:-8238216 MsaT000625.1:CDS
AGACATTGTGTGCTATTGAC+AGG 0.525810 1:-8236123 MsaT000625.1:CDS
CTTGCTGATGAAATGGGACT+TGG 0.527291 1:-8236879 MsaT000625.1:intron
TGTATACTTCGCAGGAGATG+TGG 0.527402 1:+8235133 None:intergenic
ATACATACAACATTCATTTG+CGG 0.527977 1:+8236619 None:intergenic
CAACAATATGAACATAGCCC+CGG 0.529469 1:-8237092 MsaT000625.1:CDS
GTATTGGACTTACACATTCA+GGG 0.529829 1:+8238409 None:intergenic
GGGAGCGCGAATTTGAAAGA+TGG 0.531612 1:-8236645 MsaT000625.1:CDS
GTATACTTCGCAGGAGATGT+GGG 0.532884 1:+8235134 None:intergenic
GCATATAAAGTGGGAGTGCA+TGG 0.533080 1:-8236366 MsaT000625.1:intron
GAGAAACATTCTCTTTGCAA+AGG 0.538799 1:+8236707 None:intergenic
TCCACTTTCGCCGGCAACAG+TGG 0.540259 1:+8238270 None:intergenic
AGTAGCTTGGTTGACAGGCT+TGG 0.540263 1:-8239352 MsaT000625.1:CDS
AAGGATTCATGTCAAGCTTG+TGG 0.544104 1:-8238534 MsaT000625.1:CDS
AGAAACATTCTCTTTGCAAA+GGG 0.545302 1:+8236708 None:intergenic
AGGTGAAGCTTCCAGTTCCT+GGG 0.547166 1:+8215535 None:intergenic
AAGTATAACATGAGTTTGCT+TGG 0.551969 1:+8236899 None:intergenic
AATCTAGTGCTCGTAGATGA+AGG 0.554052 1:-8215666 MsaT000625.1:CDS
TTTGTTTAATACAGGGACAA+TGG 0.554370 1:-8237317 MsaT000625.1:intron
GGAGCGCGAATTTGAAAGAT+GGG 0.560761 1:-8236644 MsaT000625.1:CDS
TACTAACCTTCCCAGCATCA+AGG 0.561391 1:+8235336 None:intergenic
TTATGCACTTCCTTGATGCT+GGG 0.561542 1:-8235346 MsaT000625.1:CDS
AGTGGAGCAACCACCAGATG+TGG 0.562409 1:+8236685 None:intergenic
AGGTCATGCGGGTTACTTGT+GGG 0.563563 1:-8215646 MsaT000625.1:CDS
CAATATCTTGTGAAGTGGAA+AGG 0.567850 1:-8238211 MsaT000625.1:CDS
GCACATCCAGTTGTCAAGAC+GGG 0.570209 1:+8238431 None:intergenic
CATCATTGAAGCATATAAAG+TGG 0.571016 1:-8236376 MsaT000625.1:CDS
AGAAGGAATATCTTGTGAAG+TGG 0.573172 1:-8237289 MsaT000625.1:CDS
TCTTATCAACATCAATAAGT+GGG 0.573695 1:+8238314 None:intergenic
GGCACATCCAGTTGTCAAGA+CGG 0.576340 1:+8238430 None:intergenic
CATTCGACCTGAATGGACTA+CGG 0.580178 1:-8237869 MsaT000625.1:CDS
CGTAGTCCATTCAGGTCGAA+TGG 0.580380 1:+8237870 None:intergenic
CTCGTAGATGAAGGTCATGC+GGG 0.584677 1:-8215657 MsaT000625.1:CDS
AATGCATAGAGCCCAAATGT+TGG 0.585664 1:+8215511 None:intergenic
CCACTTTCGCCGGCAACAGT+GGG 0.591104 1:+8238271 None:intergenic
AAGGTCATGCGGGTTACTTG+TGG 0.594310 1:-8215647 MsaT000625.1:CDS
GAATGTTTCTCCACATCTGG+TGG 0.596370 1:-8236695 MsaT000625.1:CDS
ATCATTGAAGCATATAAAGT+GGG 0.597898 1:-8236375 MsaT000625.1:CDS
TACATGCAGATAGTTGATGA+AGG 0.599170 1:-8236204 MsaT000625.1:intron
CTTTATATGCTTCAATGATG+CGG 0.599988 1:+8236379 None:intergenic
GCACTTATTCATTCCACACC+GGG 0.601453 1:-8238479 MsaT000625.1:CDS
CATAGAGCCCAAATGTTGGA+AGG 0.603604 1:+8215515 None:intergenic
ACTACATCCATATCAACTTG+AGG 0.610933 1:-8236949 MsaT000625.1:CDS
TGCACTTATTCATTCCACAC+CGG 0.617206 1:-8238480 MsaT000625.1:CDS
GCCAGAGGAAGAGTTTCTGA+AGG 0.618618 1:-8237983 MsaT000625.1:CDS
ACTTTCTACACTACAAAACT+GGG 0.626881 1:-8236665 MsaT000625.1:CDS
AGCCCCGGATTTCTTTCAGG+GGG 0.630635 1:-8237077 MsaT000625.1:intron
GAACTCGGGAAAAGTTTGAG+AGG 0.631279 1:-8239249 MsaT000625.1:CDS
AAGTGGTGACAGTAAACACC+CGG 0.636122 1:+8238461 None:intergenic
GCTCGTAGATGAAGGTCATG+CGG 0.639649 1:-8215658 MsaT000625.1:CDS
ATAATAGATAGGTTGAGACT+AGG 0.648362 1:-8223852 MsaT000625.1:intron
GCTTGGACCAAGAGAAACGT+AGG 0.652671 1:+8236916 None:intergenic
TGTCAAGCTTGTGGAAAGAG+CGG 0.655768 1:-8238525 MsaT000625.1:CDS
TATCGTATATGCATTGCGGA+TGG 0.656435 1:-8238186 MsaT000625.1:intron
TATCTGACTCAAGCTCCCAA+TGG 0.656670 1:+8237243 None:intergenic
GGACTATCGTATATGCATTG+CGG 0.680748 1:-8238190 MsaT000625.1:CDS
GTGACAGTAAACACCCGGTG+TGG 0.682101 1:+8238466 None:intergenic
TATACTTCGCAGGAGATGTG+GGG 0.683459 1:+8235135 None:intergenic
TAGCCCCGGATTTCTTTCAG+GGG 0.691300 1:-8237078 MsaT000625.1:CDS
AAAGTGGATCCGAAAATTTG+GGG 0.760715 1:-8238255 MsaT000625.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr1 gene 8215491 8239377 8215491 ID=MsaG000625
Chr1 mRNA 8215491 8239377 8215491 ID=MsaT000625.1;Parent=MsaG000625
Chr1 exon 8215491 8215731 8215491 ID=MsaT000625.1.exon14;Parent=MsaT000625.1
Chr1 CDS 8215491 8215731 8215491 ID=cds.MsaT000625.1;Parent=MsaT000625.1
Chr1 exon 8223796 8223863 8223796 ID=MsaT000625.1.exon13;Parent=MsaT000625.1
Chr1 CDS 8223796 8223863 8223796 ID=cds.MsaT000625.1;Parent=MsaT000625.1
Chr1 exon 8231495 8231565 8231495 ID=MsaT000625.1.exon12;Parent=MsaT000625.1
Chr1 CDS 8231495 8231565 8231495 ID=cds.MsaT000625.1;Parent=MsaT000625.1
Chr1 exon 8235140 8235239 8235140 ID=MsaT000625.1.exon11;Parent=MsaT000625.1
Chr1 CDS 8235140 8235239 8235140 ID=cds.MsaT000625.1;Parent=MsaT000625.1
Chr1 exon 8235343 8235393 8235343 ID=MsaT000625.1.exon10;Parent=MsaT000625.1
Chr1 CDS 8235343 8235393 8235343 ID=cds.MsaT000625.1;Parent=MsaT000625.1
Chr1 exon 8236110 8236217 8236110 ID=MsaT000625.1.exon9;Parent=MsaT000625.1
Chr1 CDS 8236110 8236217 8236110 ID=cds.MsaT000625.1;Parent=MsaT000625.1
Chr1 exon 8236367 8236552 8236367 ID=MsaT000625.1.exon8;Parent=MsaT000625.1
Chr1 CDS 8236367 8236552 8236367 ID=cds.MsaT000625.1;Parent=MsaT000625.1
Chr1 exon 8236627 8236763 8236627 ID=MsaT000625.1.exon7;Parent=MsaT000625.1
Chr1 CDS 8236627 8236763 8236627 ID=cds.MsaT000625.1;Parent=MsaT000625.1
Chr1 exon 8236880 8236975 8236880 ID=MsaT000625.1.exon6;Parent=MsaT000625.1
Chr1 CDS 8236880 8236975 8236880 ID=cds.MsaT000625.1;Parent=MsaT000625.1
Chr1 exon 8237078 8237325 8237078 ID=MsaT000625.1.exon5;Parent=MsaT000625.1
Chr1 CDS 8237078 8237325 8237078 ID=cds.MsaT000625.1;Parent=MsaT000625.1
Chr1 exon 8237847 8238008 8237847 ID=MsaT000625.1.exon4;Parent=MsaT000625.1
Chr1 CDS 8237847 8238008 8237847 ID=cds.MsaT000625.1;Parent=MsaT000625.1
Chr1 exon 8238187 8238341 8238187 ID=MsaT000625.1.exon3;Parent=MsaT000625.1
Chr1 CDS 8238187 8238341 8238187 ID=cds.MsaT000625.1;Parent=MsaT000625.1
Chr1 exon 8238422 8238559 8238422 ID=MsaT000625.1.exon2;Parent=MsaT000625.1
Chr1 CDS 8238422 8238559 8238422 ID=cds.MsaT000625.1;Parent=MsaT000625.1
Chr1 exon 8239231 8239377 8239231 ID=MsaT000625.1.exon1;Parent=MsaT000625.1
Chr1 CDS 8239231 8239377 8239231 ID=cds.MsaT000625.1;Parent=MsaT000625.1
Gene Sequence

>MsaT000625.1

ATGAGTAGCTTGGTTGACAGGCTTGGTGTAAGATCTGATCGAAAGCAATTTTATACTCAAGATGAGTTAGACGATGATGCTGATTTAATGCCTAGAAAAAGTCAGAGAACTCGGGAAAAGTTTGAGAGGATTGTAAGAAGCGATGCAAAAAAGGATTCATGTCAAGCTTGTGGAAAGAGCGGAAATCTTCTGAGCTGTAAAAGATGCACTTATTCATTCCACACCGGGTGTTTACTGTCACCACTTAAAAGCTCCCGTCTTGACAACTGGATGTGCCCTGAATGTGTTAGCCCACTTATTGATGTTGATAAGATATTAGATTGTAAGATGCGTCCCACTGTTGCCGGCGAAAGTGGATCCGAAAATTTGGGGTCCAAACAATGTTTGACGAAACAATATCTTGTGAAGTGGAAAGGACTATCGTATATGCATTGCGGATGGGTGCCAGAGGAAGAGTTTCTGAAGGCTTTCAAGCTCAATCATCATTTTAAGACCAAAGTAAACAACTTTCATCGTCAAATGGCACAAGTCAGAAAACTGGATGAAGATTTCGTTGCCATTCGACCTGAATGGACTACGGTTCATCGAATACTTGCTTGCAGGGACAATGGTGCTGAGAAGGAATATCTTGTGAAGTGGAAAGAGCTTCCTTATGATGAATGCCATTGGGAGCTTGAGTCAGATATCTCTTTGTTTCAAGCAGAAATAGAGAGATTCAATATATTTCGTTCTAGGTCTAGAAAGATTTCTTTTAGTAAGCAAAAAAACAGTGTTAGTGATGATGCTGAATTGATAAAGCAGCAGAATGAATTTCAACAATATGAACATAGCCCCGGATTTCTTTCAGGGGGTACACTACATCCATATCAACTTGAGGGATTGAACTTCCTACGTTTCTCTTGGTCCAAGCAAACTCATGTTATACTTGCTGATGAAATGGGACTTGGAAAAACCATACAAAGTATTGCTTTCTTAGCATCCCTTTGCAAAGAGAATGTTTCTCCACATCTGGTGGTTGCTCCACTTTCTACACTACAAAACTGGGAGCGCGAATTTGAAAGATGGGCACCGCAAATGAATGTTGTTATGTATGTTGGATCTTTCCAAGCTCGTAGCACTATTAGAGAATATGAATTTTACTTTCCGAAGAATCCGAAGAATTCTAAAAATGAATTTCTGGAAAGCATTAAGTTTGATGTTCTTTTGACATCATATGAGATTATAAACCAAGATGCCGCATCATTGAAGCATATAAAGTGGGAGTGCATGATAGTTGATGAAGGTCACCGTCTGAAAAACAAGGATTCAAAATTGTTTTTTTCATTGAAGCAATACTCTAGCAGACATTGTGTGCTATTGACAGGAACTCCTCTTCAGAACAATCTGGATGAACTCTTTATGCTTATGCACTTCCTTGATGCTGGGAAGTTTGGAAGTTTAGAAGAGTTCCAAGAAGAGTTTAAGGACATCAATCAAGAGCAACAGGTTTCGAGGCTTCATACATTGTTGGCCCCACATCTCCTGCGAATATGCAGATCAACATTTTCATCTAGTAGTCTAAAAGAATGGCAGAAACATGCTAGTAAAAAAAACTTCAAAGTTGAGACTAGGAAATTGAAGATTCACAAGACAACATTAGAAGCTGAAAGTTACAAACAAAAGATTTTATCAACACCAAAAAACAGGACTCTGAAGCTCCTAGATTCTGGGAATCTAGTGCTCGTAGATGAAGGTCATGCGGGTTACTTGTGGGAGAGTTTCAAGCAACCAACAGACACATTAATTTTTGGAATGGCATTAGATAAAAATATGGCATTGATAACTTGGAAAAACGAGAATGACCCAGGAACTGGAAGCTTCACCTTCCAACATTTGGGCTCTATGCATTACTTAATGAAATACCACTAA
Protein sequence

>MsaT000625.1

MSSLVDRLGVRSDRKQFYTQDELDDDADLMPRKSQRTREKFERIVRSDAKKDSCQACGKSGNLLSCKRCTYSFHTGCLLSPLKSSRLDNWMCPECVSPLIDVDKILDCKMRPTVAGESGSENLGSKQCLTKQYLVKWKGLSYMHCGWVPEEEFLKAFKLNHHFKTKVNNFHRQMAQVRKLDEDFVAIRPEWTTVHRILACRDNGAEKEYLVKWKELPYDECHWELESDISLFQAEIERFNIFRSRSRKISFSKQKNSVSDDAELIKQQNEFQQYEHSPGFLSGGTLHPYQLEGLNFLRFSWSKQTHVILADEMGLGKTIQSIAFLASLCKENVSPHLVVAPLSTLQNWEREFERWAPQMNVVMYVGSFQARSTIREYEFYFPKNPKNSKNEFLESIKFDVLLTSYEIINQDAASLKHIKWECMIVDEGHRLKNKDSKLFFSLKQYSSRHCVLLTGTPLQNNLDELFMLMHFLDAGKFGSLEEFQEEFKDINQEQQVSRLHTLLAPHLLRICRSTFSSSSLKEWQKHASKKNFKVETRKLKIHKTTLEAESYKQKILSTPKNRTLKLLDSGNLVLVDEGHAGYLWESFKQPTDTLIFGMALDKNMALITWKNENDPGTGSFTFQHLGSMHYLMKYH*