AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG000673

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG000673 MtrunA17_Chr1g0149451 91.853 626 25 3 1 600 16 641 0.0 1172
MsaG000673 MtrunA17_Chr3g0082141 31.461 267 157 4 46 287 171 436 2.50e-35 142
MsaG000673 MtrunA17_Chr1g0164561 33.333 219 120 4 94 287 7 224 3.05e-34 130
MsaG000673 MtrunA17_Chr4g0047191 29.293 198 132 3 95 291 121 311 5.42e-20 92.0
MsaG000673 MtrunA17_Chr4g0047211 29.293 198 132 3 95 291 116 306 6.48e-20 91.7
MsaG000673 MtrunA17_Chr5g0406251 26.007 273 179 9 19 287 298 551 1.49e-18 89.4
MsaG000673 MtrunA17_Chr4g0047201 28.283 198 134 3 95 291 277 467 2.78e-18 88.2
MsaG000673 MtrunA17_Chr2g0282321 29.293 198 132 3 95 291 238 428 8.39e-18 86.7
MsaG000673 MtrunA17_Chr5g0427651 25.455 275 185 7 20 289 650 909 9.53e-16 81.3
MsaG000673 MtrunA17_Chr1g0211611 26.275 255 171 9 46 295 323 565 9.67e-16 80.5
MsaG000673 MtrunA17_Chr3g0133541 26.642 274 179 8 22 286 313 573 1.05e-15 80.9
MsaG000673 MtrunA17_Chr3g0132791 26.882 279 181 8 21 291 658 921 1.54e-15 80.5
MsaG000673 MtrunA17_Chr3g0117811 24.490 196 141 3 87 281 146 335 1.35e-14 75.9
MsaG000673 MtrunA17_Chr2g0286501 35.766 137 83 4 106 239 126 260 3.86e-14 76.3
MsaG000673 MtrunA17_Chr4g0025041 28.931 159 100 5 122 278 98 245 4.07e-14 74.7
MsaG000673 MtrunA17_Chr5g0394681 26.087 184 129 3 95 277 181 358 4.72e-14 74.3
MsaG000673 MtrunA17_Chr1g0205071 23.810 189 137 3 95 282 173 355 5.52e-14 73.9
MsaG000673 MtrunA17_Chr6g0482711 31.056 161 103 4 123 281 759 913 2.12e-13 73.6
MsaG000673 MtrunA17_Chr4g0075541 25.397 189 133 3 95 282 162 343 2.57e-13 72.0
MsaG000673 MtrunA17_Chr7g0258051 23.333 270 188 7 10 278 15 266 2.67e-13 72.8
MsaG000673 MtrunA17_Chr5g0397931 28.977 176 109 5 123 288 522 691 5.01e-13 72.0
MsaG000673 MtrunA17_Chr8g0392871 25.094 267 178 8 20 281 743 992 6.39e-13 72.0
MsaG000673 MtrunA17_Chr3g0143531 23.048 269 172 5 19 277 28 271 2.32e-12 68.2
MsaG000673 MtrunA17_Chr1g0179331 30.488 164 106 4 123 284 656 813 4.20e-12 69.3
MsaG000673 MtrunA17_Chr8g0386981 26.217 267 171 9 20 278 412 660 1.70e-11 67.4
MsaG000673 MtrunA17_Chr3g0103791 24.670 227 151 6 8 225 115 330 1.75e-11 66.6
MsaG000673 MtrunA17_Chr2g0299991 26.000 200 129 7 91 278 138 330 2.22e-11 65.9
MsaG000673 MtrunA17_Chr1g0191171 25.468 267 177 8 20 281 693 942 2.56e-11 67.0
MsaG000673 MtrunA17_Chr5g0424501 27.381 168 108 5 111 277 286 440 3.14e-11 65.9
MsaG000673 MtrunA17_Chr2g0288841 26.603 312 167 15 22 304 11 289 9.70e-11 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG000673 AT4G32250.3 65.498 542 178 6 20 556 43 580 0.0 722
MsaG000673 AT4G32250.2 65.498 542 178 6 20 556 43 580 0.0 722
MsaG000673 AT4G32250.1 65.498 542 178 6 20 556 43 580 0.0 722
MsaG000673 AT5G13530.2 32.075 265 155 6 46 287 171 433 4.55e-36 145
MsaG000673 AT5G13530.1 32.075 265 155 6 46 287 171 433 4.64e-36 145
MsaG000673 AT4G38470.2 24.818 274 183 9 20 289 294 548 1.42e-16 83.6
MsaG000673 AT1G05100.1 32.044 181 106 7 103 277 89 258 2.40e-16 81.3
MsaG000673 AT4G38470.1 25.641 273 176 10 20 288 294 543 3.80e-16 82.4
MsaG000673 AT3G63260.1 25.521 192 135 3 92 281 178 363 9.10e-16 80.1
MsaG000673 AT4G24480.1 27.273 286 180 9 20 292 673 943 1.45e-15 80.9
MsaG000673 AT4G26890.1 28.108 185 119 4 96 278 77 249 1.10e-14 77.0
MsaG000673 AT4G24480.3 27.305 282 180 9 20 292 673 938 1.17e-14 78.2
MsaG000673 AT4G24480.2 27.305 282 180 9 20 292 673 938 1.25e-14 78.2
MsaG000673 AT5G01850.1 27.861 201 130 5 94 287 90 282 1.50e-14 75.5
MsaG000673 AT5G01850.2 27.861 201 130 5 94 287 114 306 1.68e-14 75.9
MsaG000673 AT3G46930.1 26.131 199 138 4 95 291 240 431 3.14e-14 75.9
MsaG000673 AT3G46930.2 26.131 199 138 4 95 291 275 466 3.46e-14 75.9
MsaG000673 AT4G35780.1 22.826 276 190 9 19 290 295 551 4.84e-14 75.5
MsaG000673 AT1G62400.1 25.253 198 139 4 95 291 161 350 1.66e-13 73.2
MsaG000673 AT3G01490.1 23.684 190 137 3 94 281 200 383 2.66e-13 72.4
MsaG000673 AT1G08720.1 26.471 272 177 8 21 287 674 927 3.85e-13 73.2
MsaG000673 AT5G58950.1 25.126 199 141 3 95 292 284 475 6.75e-13 72.0
MsaG000673 AT4G38470.3 26.106 226 144 9 20 241 294 500 8.15e-13 71.6
MsaG000673 AT1G32320.1 26.493 268 163 11 22 278 54 298 8.51e-13 70.1
MsaG000673 AT1G73660.1 24.254 268 181 7 20 282 752 1002 1.25e-12 71.6
MsaG000673 AT5G11850.1 24.627 268 180 7 20 282 613 863 1.88e-12 70.9
MsaG000673 AT2G42640.4 28.571 182 118 6 100 281 567 736 1.94e-12 70.9
MsaG000673 AT2G42640.1 28.571 182 118 6 100 281 605 774 2.23e-12 70.5
MsaG000673 AT4G23050.2 29.299 157 103 4 123 277 567 717 9.15e-12 68.6
MsaG000673 AT1G18160.1 24.254 268 181 7 20 282 719 969 9.23e-12 68.9
MsaG000673 AT4G23050.1 29.299 157 103 4 123 277 566 716 9.30e-12 68.6
MsaG000673 AT4G29810.3 27.363 201 125 8 91 280 147 337 1.30e-11 66.6
MsaG000673 AT5G51270.2 25.217 230 119 9 19 224 449 649 1.96e-11 67.8
MsaG000673 AT5G51270.1 25.217 230 119 9 19 224 449 649 1.96e-11 67.8
MsaG000673 AT3G45790.1 28.372 215 138 5 22 226 102 310 2.38e-11 66.2
MsaG000673 AT4G29810.1 27.136 199 124 8 91 278 138 326 3.83e-11 65.5
MsaG000673 AT4G29810.2 27.136 199 124 8 91 278 147 335 4.77e-11 65.5
MsaG000673 AT5G03730.2 25.714 280 184 10 9 281 544 806 6.46e-11 65.9
MsaG000673 AT5G03730.1 25.714 280 184 10 9 281 544 806 6.46e-11 65.9
MsaG000673 AT3G46140.1 27.907 215 139 5 22 226 102 310 8.85e-11 64.7

Find 171 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCATGAGTTGGGAGGCTTTC+TGG 0.085084 1:+8846389 None:intergenic
TGACAACGATCAAGCGATTT+TGG 0.146585 1:-8847411 MsaT000673.1:CDS
GATTCGAATGGCGTCGTAAA+AGG 0.159414 1:-8846008 MsaT000673.1:CDS
GAAGAACATTACATGGTTTA+AGG 0.171246 1:+8847438 None:intergenic
ACCTCTGGTTTCCATTGTTC+TGG 0.192206 1:+8847299 None:intergenic
AGAGCTTCTGTTGGATTTAT+TGG 0.203694 1:-8846132 MsaT000673.1:CDS
CATGGAATTTCAATGTTAAA+TGG 0.209362 1:-8847811 MsaT000673.1:CDS
GTTAAGCACCATCTTTAAAA+AGG 0.241242 1:+8845593 None:intergenic
TGTTCTGGAGCCATGTAATT+AGG 0.250797 1:+8847314 None:intergenic
TTAGAACGTACTGCAGATTA+TGG 0.302417 1:-8846324 MsaT000673.1:CDS
GGGCTTAACACATTCTCTTT+TGG 0.303938 1:+8846033 None:intergenic
GAGCTTCTGTTGGATTTATT+GGG 0.309467 1:-8846131 MsaT000673.1:CDS
TTTCCAGGGATGGTGAGTTT+TGG 0.309534 1:-8845922 MsaT000673.1:CDS
ATATTCTGCGCGTCTTTAAA+AGG 0.310548 1:-8847049 MsaT000673.1:intron
GTCGATGTATGAATTCTTAT+AGG 0.311891 1:+8846267 None:intergenic
TGTTTGGTTGTCAAGTTTCC+AGG 0.313479 1:-8845937 MsaT000673.1:CDS
TAGTCAGTGCTGCCTGATTT+TGG 0.315215 1:+8846465 None:intergenic
TTCACATGATCCTCTTTGAT+TGG 0.322968 1:+8847901 None:intergenic
TCTGCGTGGGCCAGTTTGTT+AGG 0.324940 1:-8846056 MsaT000673.1:CDS
CTGCTACTGGTAGGATTTCA+TGG 0.337558 1:-8845975 MsaT000673.1:CDS
GAGGACCCTTTGGTGATGTT+TGG 0.361104 1:-8847993 MsaT000673.1:CDS
CCATGGGTTATAATCCTTGT+AGG 0.362031 1:+8845652 None:intergenic
TTCTAGAGCTTCACGCTAAA+GGG 0.362481 1:-8847475 MsaT000673.1:CDS
AAGGAGTTTCTAGTGTTTGC+TGG 0.368878 1:-8847837 MsaT000673.1:CDS
ACATCTTAGCAGCAACTTCA+TGG 0.384443 1:+8847929 None:intergenic
TCTACTGTCATTGCGATTGA+TGG 0.391209 1:+8846152 None:intergenic
TTGAAACTTCAGCACCGAAT+TGG 0.395091 1:-8848018 MsaT000673.1:intron
GAAACGGATTCATGGGGATT+CGG 0.395952 1:-8847257 MsaT000673.1:CDS
ATTCGAATGGCGTCGTAAAA+GGG 0.398237 1:-8846007 MsaT000673.1:CDS
CCTCTCAAAGGATCAACTTC+AGG 0.398972 1:-8846430 MsaT000673.1:CDS
TTGAAACTAGGCATATTAGT+TGG 0.401044 1:-8845751 MsaT000673.1:CDS
TCGATGTATGAATTCTTATA+GGG 0.403661 1:+8846268 None:intergenic
CGAATTGGTCGAGGACCCTT+TGG 0.406343 1:-8848003 MsaT000673.1:CDS
ATATTTACAGCTTTGAAACT+AGG 0.412019 1:-8845763 MsaT000673.1:CDS
TGAAGAGCCAAGCAAAAGAT+TGG 0.413672 1:+8847373 None:intergenic
ATGAAAGTAATTCCAAAATC+AGG 0.416519 1:-8846477 MsaT000673.1:CDS
ATGGCCTGGCAGCTGGTGAT+TGG 0.421439 1:-8846227 MsaT000673.1:CDS
TTATTGGGCTGCAAACTCTC+TGG 0.426673 1:-8846116 MsaT000673.1:CDS
AGGGGAGGGGCCTCTGCTAC+TGG 0.429138 1:-8845988 MsaT000673.1:CDS
TTGACAACCAAACACCCATT+TGG 0.430784 1:+8845946 None:intergenic
ATGCCTAGTACAACTAGAAC+AGG 0.431258 1:+8845784 None:intergenic
TGTCATTTGAAACGGATTCA+TGG 0.434134 1:-8847265 MsaT000673.1:CDS
GGAGGAGGATGGAGATATCA+AGG 0.438936 1:-8846504 MsaT000673.1:CDS
TGTCCTGTTGGTGAAATATA+CGG 0.443667 1:-8847188 MsaT000673.1:CDS
GATACTACCAAATGGGTGTT+TGG 0.448805 1:-8845953 MsaT000673.1:CDS
TTCCCAAAACTCACCATCCC+TGG 0.449001 1:+8845919 None:intergenic
GTCATTTGAAACGGATTCAT+GGG 0.449610 1:-8847264 MsaT000673.1:CDS
ATTCTAGAGCTTCACGCTAA+AGG 0.452406 1:-8847476 MsaT000673.1:CDS
TTTGAGGAATCGACCTTCAA+TGG 0.455368 1:-8847075 MsaT000673.1:CDS
GTCACGAATGGCCTGGCAGC+TGG 0.457715 1:-8846234 MsaT000673.1:CDS
TCTAGTGTTTGCTGGTTACA+TGG 0.459137 1:-8847829 MsaT000673.1:CDS
TCTCCCATTGAGAATATCCT+TGG 0.462519 1:-8847116 MsaT000673.1:CDS
CAAAGGGTCCTCGACCAATT+CGG 0.467685 1:+8848004 None:intergenic
ATTTCATGGATACTACCAAA+TGG 0.474128 1:-8845961 MsaT000673.1:CDS
GACATAGGGCCTCTGACCTC+TGG 0.481700 1:+8847284 None:intergenic
GGTTGTCAAGTTTCCAGGGA+TGG 0.483297 1:-8845932 MsaT000673.1:CDS
AGTTGGGAGGCTTTCTGGAT+CGG 0.491406 1:+8846394 None:intergenic
AAGCCAACGACAGTTCCGTC+TGG 0.492923 1:+8846345 None:intergenic
AGGCCCTATGTCATTTGAAA+CGG 0.494592 1:-8847273 MsaT000673.1:CDS
GAAGAGCCAAGCAAAAGATT+GGG 0.495194 1:+8847374 None:intergenic
AAACCCTTCAGAACTCGAAA+TGG 0.495513 1:-8846088 MsaT000673.1:CDS
AAATTGTATGACGGTTCAAT+TGG 0.497991 1:-8847674 MsaT000673.1:CDS
CTGAATGAACTGGCTAATGA+TGG 0.498348 1:-8846525 MsaT000673.1:CDS
GGTACGCTTGAAAGATGAAA+AGG 0.498765 1:-8846205 MsaT000673.1:CDS
AGACCTGTTCTAGTTGTACT+AGG 0.500576 1:-8845787 MsaT000673.1:CDS
TAACCCATGGCAACACAACA+TGG 0.500752 1:-8845639 MsaT000673.1:CDS
GATCCGTATATTTCACCAAC+AGG 0.502608 1:+8847185 None:intergenic
GAGGGGCCTCTGCTACTGGT+AGG 0.503223 1:-8845984 MsaT000673.1:CDS
TGGCCTGGCAGCTGGTGATT+GGG 0.503490 1:-8846226 MsaT000673.1:CDS
ATGGGAGAAGGAAGGCCACT+TGG 0.504464 1:+8847131 None:intergenic
AACTTCAACCTCGGAAGGAT+CGG 0.506263 1:+8845879 None:intergenic
TGCACCAAGGATATTCTCAA+TGG 0.506987 1:+8847112 None:intergenic
CGCAGAATATCTACCATTGA+AGG 0.507993 1:+8847062 None:intergenic
GAATTTCAATGTTAAATGGA+AGG 0.512178 1:-8847807 MsaT000673.1:CDS
CTCAAAGGATCAACTTCAGG+TGG 0.513886 1:-8846427 MsaT000673.1:CDS
TTATGGATTTGTTCTAGTGA+GGG 0.514209 1:-8846307 MsaT000673.1:CDS
TCAGATATTGCTCAGAGGCT+TGG 0.514336 1:-8847341 MsaT000673.1:CDS
GTTTGGTTGTCAAGTTTCCA+GGG 0.514420 1:-8845936 MsaT000673.1:CDS
ACACATCACCTCAAAACATC+AGG 0.516904 1:+8847611 None:intergenic
ATGGTTTAAGGTTGAGAACA+AGG 0.524109 1:+8847450 None:intergenic
AATTTCAATGTTAAATGGAA+GGG 0.525630 1:-8847806 MsaT000673.1:intron
TTGCAGGTCACTGAATGAAC+TGG 0.528545 1:-8846535 MsaT000673.1:intron
TCTGCCATTTCGAGTTCTGA+AGG 0.531466 1:+8846084 None:intergenic
GTACCCAATCACCAGCTGCC+AGG 0.532200 1:+8846223 None:intergenic
TTTCATGGATACTACCAAAT+GGG 0.532985 1:-8845960 MsaT000673.1:CDS
AGCAAACACTAGAAACTCCT+TGG 0.533146 1:+8847839 None:intergenic
TGAACGCGTCACGAATGGCC+TGG 0.537908 1:-8846241 MsaT000673.1:CDS
CACCGAGTGGTTCCTCTCAA+AGG 0.541938 1:-8846442 MsaT000673.1:CDS
CCTTGGTATGGATGTCCTGT+TGG 0.543914 1:-8847200 MsaT000673.1:CDS
ATTATGGATTTGTTCTAGTG+AGG 0.545782 1:-8846308 MsaT000673.1:CDS
TGGCAGGTATGGGATCGATC+TGG 0.548820 1:-8847507 MsaT000673.1:intron
TACGACGCCATTCGAATCGA+GGG 0.550381 1:+8846013 None:intergenic
GAATCCGTTTCAAATGACAT+AGG 0.550959 1:+8847269 None:intergenic
TCATTGAGAAGAACATTACA+TGG 0.551201 1:+8847431 None:intergenic
GCACCAAGGATATTCTCAAT+GGG 0.552742 1:+8847113 None:intergenic
TTCGAATGGCGTCGTAAAAG+GGG 0.556323 1:-8846006 MsaT000673.1:CDS
TAGTTGGAAACAAAGTGAAA+AGG 0.558036 1:-8845735 MsaT000673.1:CDS
AACATGTTGAGGATCATCAC+TGG 0.559885 1:-8845816 MsaT000673.1:CDS
AAAATCAACAACTTCAACCT+CGG 0.561609 1:+8845870 None:intergenic
ACTGGTAGTCAACCTTGGTA+TGG 0.562801 1:-8847212 MsaT000673.1:CDS
AAAAGGATATTAGCAACTGA+AGG 0.563136 1:+8845610 None:intergenic
TGAGATTTGCATGAGTTGGG+AGG 0.563242 1:+8846381 None:intergenic
AACCCATGGCAACACAACAT+GGG 0.563805 1:-8845638 MsaT000673.1:CDS
CTGCCATTTCGAGTTCTGAA+GGG 0.564065 1:+8846085 None:intergenic
AGCCAACGACAGTTCCGTCT+GGG 0.566135 1:+8846346 None:intergenic
GGGCTGCAAACTCTCTGGAA+TGG 0.566139 1:-8846111 MsaT000673.1:CDS
ACTCTTGAACGCGTCACGAA+TGG 0.567776 1:-8846246 MsaT000673.1:CDS
GTCAAAACAACAGTCCTACA+AGG 0.572453 1:-8845666 MsaT000673.1:CDS
TGGCTAATGATGGAGGAGGA+TGG 0.574319 1:-8846515 MsaT000673.1:CDS
ACCCATGGCAACACAACATG+GGG 0.575393 1:-8845637 MsaT000673.1:CDS
ATTACATGGCTCCAGAACAA+TGG 0.575533 1:-8847310 MsaT000673.1:CDS
TCAACTTCAGGTGGGTGACA+TGG 0.577067 1:-8846418 MsaT000673.1:CDS
AGAAAAGTATCAACATGTTG+AGG 0.578832 1:-8845827 MsaT000673.1:CDS
TGCAAGCCAAACATCACCAA+AGG 0.581260 1:+8847987 None:intergenic
AGGATATTCTCAATGGGAGA+AGG 0.581766 1:+8847119 None:intergenic
TTTGTTCTAGTGAGGGTCCA+CGG 0.582005 1:-8846300 MsaT000673.1:CDS
TGGGAATGAATCAAACACCT+TGG 0.582420 1:-8845902 MsaT000673.1:CDS
TCAACAACTTCAACCTCGGA+AGG 0.582617 1:+8845874 None:intergenic
TGGGAGAAGGAAGGCCACTT+GGG 0.586231 1:+8847132 None:intergenic
CGGTTCAATTGGTGACAAGT+TGG 0.586939 1:-8847663 MsaT000673.1:CDS
AATCCGTTTCAAATGACATA+GGG 0.587008 1:+8847270 None:intergenic
AAGGAAAAGCACTCACCGGT+GGG 0.588053 1:-8846186 MsaT000673.1:CDS
AATGACAGTAGAGCTTCTGT+TGG 0.590285 1:-8846141 MsaT000673.1:CDS
ACAAAGTGAAAAGGTGCAAG+AGG 0.591652 1:-8845726 MsaT000673.1:CDS
CACCTTGGCCGATCCTTCCG+AGG 0.592446 1:-8845887 MsaT000673.1:CDS
AACCTCGGAAGGATCGGCCA+AGG 0.594129 1:+8845885 None:intergenic
TGGCTAGACTAAGAGATGGA+TGG 0.594983 1:-8847643 MsaT000673.1:CDS
GATGTTACATCCAATCAAAG+AGG 0.595757 1:-8847911 MsaT000673.1:CDS
TTACGACGCCATTCGAATCG+AGG 0.595769 1:+8846012 None:intergenic
GCAAGCCAAACATCACCAAA+GGG 0.597883 1:+8847988 None:intergenic
TATGGGATCGATCTGGCTCA+AGG 0.601763 1:-8847500 MsaT000673.1:CDS
GAATTATGCTTGAAGTGCCA+AGG 0.602260 1:-8847856 MsaT000673.1:CDS
CTTCAGCACCGAATTGGTCG+AGG 0.602330 1:-8848012 MsaT000673.1:intron
GCTTGGAAGTCCTAATTACA+TGG 0.604254 1:-8847324 MsaT000673.1:CDS
ATCATTATGAAATTGTATGA+CGG 0.604681 1:-8847683 MsaT000673.1:CDS
TGTTGACTGGTAGTCAACCT+TGG 0.606529 1:-8847217 MsaT000673.1:CDS
ACCGTTGTTGAAATGTTGAC+TGG 0.606786 1:-8847230 MsaT000673.1:CDS
CCTGAAGTTGATCCTTTGAG+AGG 0.610469 1:+8846430 None:intergenic
AATGGCGTCGTAAAAGGGGA+GGG 0.612881 1:-8846002 MsaT000673.1:CDS
GTCCCAGACGGAACTGTCGT+TGG 0.613432 1:-8846348 MsaT000673.1:CDS
TGTTAAGCCCTCGATTCGAA+TGG 0.618387 1:-8846020 MsaT000673.1:CDS
TCATTTGAAACGGATTCATG+GGG 0.622874 1:-8847263 MsaT000673.1:CDS
TGAAAAGGAAAAGCACTCAC+CGG 0.625000 1:-8846190 MsaT000673.1:CDS
ATGGCAGAATCATTCTGCGT+GGG 0.627438 1:-8846069 MsaT000673.1:CDS
AAGTTGGCTAGACTAAGAGA+TGG 0.628842 1:-8847647 MsaT000673.1:CDS
TGAAATCCTACCAGTAGCAG+AGG 0.629390 1:+8845978 None:intergenic
AAAGGAAAAGCACTCACCGG+TGG 0.630780 1:-8846187 MsaT000673.1:CDS
ATCCTTTGAGAGGAACCACT+CGG 0.632608 1:+8846440 None:intergenic
ATGGCGTCGTAAAAGGGGAG+GGG 0.637421 1:-8846001 MsaT000673.1:CDS
GAATGGCGTCGTAAAAGGGG+AGG 0.641779 1:-8846003 MsaT000673.1:CDS
TATTCTCAATGGGAGAAGGA+AGG 0.642358 1:+8847123 None:intergenic
CAATGGAAACCAGAGGTCAG+AGG 0.644185 1:-8847293 MsaT000673.1:CDS
ACATGTTGAGGATCATCACT+GGG 0.644328 1:-8845815 MsaT000673.1:CDS
TCAAAGGATCAACTTCAGGT+GGG 0.652726 1:-8846426 MsaT000673.1:CDS
ATGGAATGTAGAATCCCCAC+CGG 0.657028 1:+8846171 None:intergenic
GAAAAGCTGCATATCCCAAG+TGG 0.662621 1:-8847146 MsaT000673.1:CDS
ACCAGTCAACATTTCAACAA+CGG 0.664981 1:+8847229 None:intergenic
CCTACAAGGATTATAACCCA+TGG 0.667895 1:-8845652 MsaT000673.1:CDS
AATGGCAGAATCATTCTGCG+TGG 0.675086 1:-8846070 MsaT000673.1:CDS
AATGAACTGGCTAATGATGG+AGG 0.681313 1:-8846522 MsaT000673.1:CDS
CAAAACATGAATGTCCCAGA+CGG 0.682309 1:-8846360 MsaT000673.1:CDS
CCATGGCAACACAACATGGG+GGG 0.686405 1:-8845635 MsaT000673.1:CDS
TTAGCTCAGATATTGCTCAG+AGG 0.694542 1:-8847346 MsaT000673.1:CDS
AGGAAAAGCACTCACCGGTG+GGG 0.698636 1:-8846185 MsaT000673.1:CDS
GAACTGGCTAATGATGGAGG+AGG 0.699870 1:-8846519 MsaT000673.1:CDS
TCCAGAACAATGGAAACCAG+AGG 0.702393 1:-8847300 MsaT000673.1:CDS
CATACTCAAAACATGCACCA+AGG 0.704149 1:+8847099 None:intergenic
CCCATGGCAACACAACATGG+GGG 0.707761 1:-8845636 MsaT000673.1:CDS
TTATAGGGTCATGAATACCG+TGG 0.720576 1:+8846283 None:intergenic
CCAACAGGACATCCATACCA+AGG 0.725485 1:+8847200 None:intergenic
GCAGCACTGACTACACCGAG+TGG 0.764598 1:-8846455 MsaT000673.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr1 gene 8845594 8848157 8845594 ID=MsaG000673
Chr1 mRNA 8845594 8848157 8845594 ID=MsaT000673.1;Parent=MsaG000673
Chr1 exon 8845594 8846551 8845594 ID=MsaT000673.1.exon5;Parent=MsaT000673.1
Chr1 CDS 8845594 8846551 8845594 ID=cds.MsaT000673.1;Parent=MsaT000673.1
Chr1 exon 8847050 8847523 8847050 ID=MsaT000673.1.exon4;Parent=MsaT000673.1
Chr1 CDS 8847050 8847523 8847050 ID=cds.MsaT000673.1;Parent=MsaT000673.1
Chr1 exon 8847620 8847711 8847620 ID=MsaT000673.1.exon3;Parent=MsaT000673.1
Chr1 CDS 8847620 8847711 8847620 ID=cds.MsaT000673.1;Parent=MsaT000673.1
Chr1 exon 8847807 8848028 8847807 ID=MsaT000673.1.exon2;Parent=MsaT000673.1
Chr1 CDS 8847807 8848028 8847807 ID=cds.MsaT000673.1;Parent=MsaT000673.1
Chr1 exon 8848101 8848157 8848101 ID=MsaT000673.1.exon1;Parent=MsaT000673.1
Chr1 CDS 8848101 8848157 8848101 ID=cds.MsaT000673.1;Parent=MsaT000673.1
Gene Sequence

>MsaT000673.1

ATGGCTTCTTCATCAGCTGCTTCTTTTGATTATGAGATTTTGGATAAAGATACCGACCACCGAATTGGTCGAGGACCCTTTGGTGATGTTTGGCTTGCAACTCTTCATCAGTCAACTGAAGATTATGATGAGCACCATGAAGTTGCTGCTAAGATGTTACATCCAATCAAAGAGGATCATGTGAAGATTGTATTGAAGAAATTCAATGAATTATGCTTGAAGTGCCAAGGAGTTTCTAGTGTTTGCTGGTTACATGGAATTTCAATGTTAAATGGAAGGATCTGCATCATTATGAAATTGTATGACGGTTCAATTGGTGACAAGTTGGCTAGACTAAGAGATGGATGGATCTCATTACCTGATGTTTTGAGGTATGGGATCGATCTGGCTCAAGGCATTCTAGAGCTTCACGCTAAAGGGATCCTTGTTCTCAACCTTAAACCATGTAATGTTCTTCTCAATGACAACGATCAAGCGATTTTGGGAGACGTTGGTATTCCCAATCTTTTGCTTGGCTCTTCATTCGTTAGCTCAGATATTGCTCAGAGGCTTGGAAGTCCTAATTACATGGCTCCAGAACAATGGAAACCAGAGGTCAGAGGCCCTATGTCATTTGAAACGGATTCATGGGGATTCGGATGCACCGTTGTTGAAATGTTGACTGGTAGTCAACCTTGGTATGGATGTCCTGTTGGTGAAATATACGGATCAGTTGTCGAAAAGCATGAAAAGCTGCATATCCCAAGTGGCCTTCCTTCTCCCATTGAGAATATCCTTGGTGCATGTTTTGAGTATGATTTGAGGAATCGACCTTCAATGGTAGATATTCTGCGCGTCTTTAAAAGGTCACTGAATGAACTGGCTAATGATGGAGGAGGATGGAGATATCAAGGAAACATGAAAGTAATTCCAAAATCAGGCAGCACTGACTACACCGAGTGGTTCCTCTCAAAGGATCAACTTCAGGTGGGTGACATGGTCCGATCCAGAAAGCCTCCCAACTCATGCAAATCTCAAAACATGAATGTCCCAGACGGAACTGTCGTTGGCTTAGAACGTACTGCAGATTATGGATTTGTTCTAGTGAGGGTCCACGGTATTCATGACCCTATAAGAATTCATACATCGACTCTTGAACGCGTCACGAATGGCCTGGCAGCTGGTGATTGGGTACGCTTGAAAGATGAAAAGGAAAAGCACTCACCGGTGGGGATTCTACATTCCATCAATCGCAATGACAGTAGAGCTTCTGTTGGATTTATTGGGCTGCAAACTCTCTGGAATGGAAACCCTTCAGAACTCGAAATGGCAGAATCATTCTGCGTGGGCCAGTTTGTTAGGCCAAAAGAGAATGTGTTAAGCCCTCGATTCGAATGGCGTCGTAAAAGGGGAGGGGCCTCTGCTACTGGTAGGATTTCATGGATACTACCAAATGGGTGTTTGGTTGTCAAGTTTCCAGGGATGGTGAGTTTTGGGAATGAATCAAACACCTTGGCCGATCCTTCCGAGGTTGAAGTTGTTGATTTTAACACTTGTTCTGGGATGGTAGAAAAGTATCAACATGTTGAGGATCATCACTGGGCTGTAAGACCTGTTCTAGTTGTACTAGGCATATTTACAGCTTTGAAACTAGGCATATTAGTTGGAAACAAAGTGAAAAGGTGCAAGAGGTTCAATGCAAAAGAAAGCAAAAACCAATATGTTGAAGGTCAAAACAACAGTCCTACAAGGATTATAACCCATGGCAACACAACATGGGGGGTTCCTTCAGTTGCTAATATCCTTTTTAAAGATGGTGCTTAA
Protein sequence

>MsaT000673.1

MASSSAASFDYEILDKDTDHRIGRGPFGDVWLATLHQSTEDYDEHHEVAAKMLHPIKEDHVKIVLKKFNELCLKCQGVSSVCWLHGISMLNGRICIIMKLYDGSIGDKLARLRDGWISLPDVLRYGIDLAQGILELHAKGILVLNLKPCNVLLNDNDQAILGDVGIPNLLLGSSFVSSDIAQRLGSPNYMAPEQWKPEVRGPMSFETDSWGFGCTVVEMLTGSQPWYGCPVGEIYGSVVEKHEKLHIPSGLPSPIENILGACFEYDLRNRPSMVDILRVFKRSLNELANDGGGWRYQGNMKVIPKSGSTDYTEWFLSKDQLQVGDMVRSRKPPNSCKSQNMNVPDGTVVGLERTADYGFVLVRVHGIHDPIRIHTSTLERVTNGLAAGDWVRLKDEKEKHSPVGILHSINRNDSRASVGFIGLQTLWNGNPSELEMAESFCVGQFVRPKENVLSPRFEWRRKRGGASATGRISWILPNGCLVVKFPGMVSFGNESNTLADPSEVEVVDFNTCSGMVEKYQHVEDHHWAVRPVLVVLGIFTALKLGILVGNKVKRCKRFNAKESKNQYVEGQNNSPTRIITHGNTTWGVPSVANILFKDGA*