AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG000756


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG000756 MtrunA17_Chr7g0255491 57.200 250 88 4 20 250 1 250 9.67e-80 243
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 106 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGAACAATACCTTGCCTTCT+TGG 0.194452 1:-10443790 MsaT000756.1:CDS
CCCCTCACAACACCACAATT+TGG 0.206201 1:-10443719 MsaT000756.1:CDS
TCTTGGCTTGGTTCACCTTC+TGG 0.208371 1:-10443773 MsaT000756.1:CDS
TTGTTCACATGGGCGCCAAC+TGG 0.244584 1:+10443806 None:intergenic
GGTGTTGTGAGGGGTGAATT+TGG 0.287240 1:+10443728 None:intergenic
TATAAGACATAAATCTTAAA+GGG 0.300674 1:+10443406 None:intergenic
GAAATTTGGATGCCCTTGAT+AGG 0.340355 1:+10443850 None:intergenic
CCCTCACAACACCACAATTT+GGG 0.344308 1:-10443718 MsaT000756.1:CDS
CAGGAAATTCTCTACTTATC+AGG 0.361888 1:-10444011 MsaT000756.1:CDS
TTCGCCCTTCTCCAAGGCTT+TGG 0.364197 1:+10444114 None:intergenic
AAGTTAGCACTACGGAAATT+TGG 0.364201 1:+10443836 None:intergenic
AAAGTCAAACACTTCGGGTT+CGG 0.369088 1:-10443645 MsaT000756.1:CDS
GAGGTTGGTGGTATTCCCTT+TGG 0.369855 1:+10444196 None:intergenic
AGGCTTTGGAAGTGGGCATT+GGG 0.371713 1:+10444128 None:intergenic
TTGGGCGGAAGCCAAGCCTT+AGG 0.373136 1:+10444146 None:intergenic
CTTCTCCAAGGCTTTGGAAG+TGG 0.377232 1:+10444120 None:intergenic
AAGTCAAACACTTCGGGTTC+GGG 0.381297 1:-10443644 MsaT000756.1:CDS
TGCTGCTGATGAACCTTTAC+CGG 0.384228 1:-10444071 MsaT000756.1:CDS
AATACCTTGCCTTCTTGGCT+TGG 0.386051 1:-10443785 MsaT000756.1:CDS
TTCTCTACTTATCAGGCTCT+TGG 0.389156 1:-10444004 MsaT000756.1:CDS
AGAATTTCCATCGGAAATGC+TGG 0.407959 1:-10444283 MsaT000756.1:CDS
TACTGGAGAAGGCATAATTC+AGG 0.408403 1:-10443516 MsaT000756.1:CDS
CCACAAAAGTCAAACACTTC+GGG 0.409280 1:-10443650 MsaT000756.1:CDS
GGTTCACCTTCTGGTGGCTA+TGG 0.410242 1:-10443764 MsaT000756.1:CDS
TTCTCCAAGGCTTTGGAAGT+GGG 0.410767 1:+10444121 None:intergenic
AAGGCTTTGGAAGTGGGCAT+TGG 0.413114 1:+10444127 None:intergenic
TAGGGAGTAGGACTTGAAAA+TGG 0.414467 1:+10443869 None:intergenic
GGACACGCTTATGACCGTTT+TGG 0.423141 1:+10443975 None:intergenic
GTAGGATCACGAGATAATAA+TGG 0.426831 1:+10443536 None:intergenic
AAAACGGTCATAAGCGTGTC+CGG 0.433161 1:-10443973 MsaT000756.1:CDS
TTATTATCTCGTGATCCTAC+TGG 0.438584 1:-10443533 MsaT000756.1:CDS
TTCACGTCTTGCTCCGGTAA+AGG 0.446596 1:+10444058 None:intergenic
TCAAGTCCTACTCCCTATCA+AGG 0.460041 1:-10443863 MsaT000756.1:CDS
CCCAAATTGTGGTGTTGTGA+GGG 0.460530 1:+10443718 None:intergenic
GATTAGCAGCTACGATCCTT+CGG 0.464322 1:+10443906 None:intergenic
TCACCTTCTGGTGGCTATGG+AGG 0.469395 1:-10443761 MsaT000756.1:CDS
CACCTTCTGGTGGCTATGGA+GGG 0.473697 1:-10443760 MsaT000756.1:CDS
AAACCTCAAGCGCAAAATGA+TGG 0.477017 1:-10444254 MsaT000756.1:CDS
TGGGCGGAAGCCAAGCCTTA+GGG 0.484232 1:+10444147 None:intergenic
GTTGATGATGAGGGCACATT+GGG 0.485254 1:+10443488 None:intergenic
AATTCCTTCACGTCTTGCTC+CGG 0.485955 1:+10444052 None:intergenic
TTCTCCGAGGTTGATTGAGT+TGG 0.486064 1:+10443446 None:intergenic
TGTTCACATGGGCGCCAACT+GGG 0.491409 1:+10443807 None:intergenic
GCGCAAAATGATGGAGGAGC+AGG 0.494153 1:-10444245 MsaT000756.1:CDS
TTGAGGATTTGCTGACCCTA+AGG 0.499838 1:-10444162 MsaT000756.1:CDS
GATTGAGTTGGTTCCTCTGA+GGG 0.507541 1:+10443458 None:intergenic
TTCCCTGTCAGTACTGTGAC+AGG 0.510555 1:-10444030 MsaT000756.1:CDS
TCAAACTCCTCGATTGGCTG+AGG 0.513629 1:-10443603 MsaT000756.1:CDS
GAAGGTGAACCAAGCCAAGA+AGG 0.514184 1:+10443776 None:intergenic
CACTTCCAAAGCCTTGGAGA+AGG 0.514520 1:-10444119 MsaT000756.1:CDS
TCTACTTATCAGGCTCTTGG+AGG 0.515170 1:-10444001 MsaT000756.1:CDS
GATTTGCTGACCCTAAGGCT+TGG 0.515203 1:-10444157 MsaT000756.1:CDS
AAATTTGGATGCCCTTGATA+GGG 0.517754 1:+10443851 None:intergenic
AATGCCCACTTCCAAAGCCT+TGG 0.518064 1:-10444125 MsaT000756.1:CDS
TCAACATCTGATTCAGAGGT+TGG 0.520729 1:+10444181 None:intergenic
AGCTAATCAAACTCCTCGAT+TGG 0.520876 1:-10443609 MsaT000756.1:CDS
TCCACAAAAGTCAAACACTT+CGG 0.524677 1:-10443651 MsaT000756.1:CDS
CTTGAAAATGGATGAGGGTA+TGG 0.526376 1:+10443881 None:intergenic
GAATTTCCATCGGAAATGCT+GGG 0.539209 1:-10444282 MsaT000756.1:CDS
AAAATCAGAGAAGGAGCCAA+AGG 0.542845 1:-10444212 MsaT000756.1:CDS
TGGCTTGGTTCACCTTCTGG+TGG 0.546245 1:-10443770 MsaT000756.1:CDS
GTAGGACTTGAAAATGGATG+AGG 0.549069 1:+10443875 None:intergenic
TCCCAAATTGTGGTGTTGTG+AGG 0.552382 1:+10443717 None:intergenic
TGAATTATGCCTTCTCCAGT+AGG 0.553893 1:+10443518 None:intergenic
TTTCCTGTCACAGTACTGAC+AGG 0.555350 1:+10444027 None:intergenic
ACTTCCAAAGCCTTGGAGAA+GGG 0.557599 1:-10444118 MsaT000756.1:CDS
GGATGCCCTTGATAGGGAGT+AGG 0.571516 1:+10443857 None:intergenic
CAAACTCCTCGATTGGCTGA+GGG 0.571646 1:-10443602 MsaT000756.1:CDS
TGAAGGTTCGCCCTTCTCCA+AGG 0.573870 1:+10444108 None:intergenic
TCTCGTGATCCTACTGGAGA+AGG 0.573926 1:-10443527 MsaT000756.1:CDS
GCTCTTGGAGGACACCAAAA+CGG 0.575138 1:-10443989 MsaT000756.1:CDS
ACATCTGATTCAGAGGTTGG+TGG 0.575544 1:+10444184 None:intergenic
TAGGACTTGAAAATGGATGA+GGG 0.575804 1:+10443876 None:intergenic
ATCCTCAACATCTGATTCAG+AGG 0.577188 1:+10444177 None:intergenic
AAGGCAAGGTATTGTTCACA+TGG 0.578550 1:+10443795 None:intergenic
CTTTGGAAGTGGGCATTGGG+CGG 0.581292 1:+10444131 None:intergenic
AGTTGATGATGAGGGCACAT+TGG 0.583838 1:+10443487 None:intergenic
AACCTCTGAATCAGATGTTG+AGG 0.586720 1:-10444179 MsaT000756.1:CDS
AAACGGTCATAAGCGTGTCC+GGG 0.588037 1:-10443972 MsaT000756.1:CDS
TTTGTCCCCAGCATTTCCGA+TGG 0.589355 1:+10444276 None:intergenic
GCCAGAGTCAAAATCAGAGA+AGG 0.590257 1:-10444221 MsaT000756.1:CDS
TGAACCAAGCCAAGAAGGCA+AGG 0.593315 1:+10443781 None:intergenic
AAATCAGAGAAGGAGCCAAA+GGG 0.593524 1:-10444211 MsaT000756.1:CDS
AAATTAGTCATCCCAAATTG+TGG 0.600013 1:+10443707 None:intergenic
CAAGTCCTACTCCCTATCAA+GGG 0.609231 1:-10443862 MsaT000756.1:CDS
ATAAGACATAAATCTTAAAG+GGG 0.619171 1:+10443407 None:intergenic
TGATTGAGTTGGTTCCTCTG+AGG 0.624213 1:+10443457 None:intergenic
AAGAGATAGATTTACTCCGA+AGG 0.627965 1:-10443922 MsaT000756.1:CDS
GCTAACTTGCACAACCCAGT+TGG 0.635608 1:-10443821 MsaT000756.1:CDS
ATCCCTCCATAGCCACCAGA+AGG 0.636300 1:+10443758 None:intergenic
TCTGCACCCTCAGCCAATCG+AGG 0.645887 1:+10443596 None:intergenic
TTTACCGGAGCAAGACGTGA+AGG 0.645972 1:-10444056 MsaT000756.1:CDS
TAAGACATAAATCTTAAAGG+GGG 0.653670 1:+10443408 None:intergenic
CCTCAAGCGCAAAATGATGG+AGG 0.656497 1:-10444251 MsaT000756.1:CDS
ATGGCTGAGAGAATTTCCAT+CGG 0.660705 1:-10444292 MsaT000756.1:CDS
CCAAATTGTGGTGTTGTGAG+GGG 0.669993 1:+10443719 None:intergenic
GCATTCTGAGATGAACGTGA+AGG 0.671407 1:+10444091 None:intergenic
GGAACCAACTCAATCAACCT+CGG 0.676925 1:-10443450 MsaT000756.1:CDS
AATTTCCATCGGAAATGCTG+GGG 0.677054 1:-10444281 MsaT000756.1:CDS
GGTTGTGCAAGTTAGCACTA+CGG 0.678534 1:+10443828 None:intergenic
ATCAACTCAATCACCCTCAG+AGG 0.680896 1:-10443471 MsaT000756.1:CDS
GGGTGATTGAGTTGATGATG+AGG 0.681787 1:+10443478 None:intergenic
GGTGATTGAGTTGATGATGA+GGG 0.690927 1:+10443479 None:intergenic
ATTCAAGTTGAGTTTCTCCG+AGG 0.691771 1:+10443433 None:intergenic
AGGCAAGGTATTGTTCACAT+GGG 0.701207 1:+10443796 None:intergenic
TTCCTGTCACAGTACTGACA+GGG 0.704758 1:+10444028 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr1 gene 10443421 10444314 10443421 ID=MsaG000756
Chr1 mRNA 10443421 10444314 10443421 ID=MsaT000756.1;Parent=MsaG000756
Chr1 exon 10443421 10444314 10443421 ID=MsaT000756.1.exon1;Parent=MsaT000756.1
Chr1 CDS 10443421 10444314 10443421 ID=cds.MsaT000756.1;Parent=MsaT000756.1
Gene Sequence

>MsaT000756.1

ATGGCTGAGAGAATTTCCATCGGAAATGCTGGGGACAAAAACCTCAAGCGCAAAATGATGGAGGAGCAGGAGCCAGAGTCAAAATCAGAGAAGGAGCCAAAGGGAATACCACCAACCTCTGAATCAGATGTTGAGGATTTGCTGACCCTAAGGCTTGGCTTCCGCCCAATGCCCACTTCCAAAGCCTTGGAGAAGGGCGAACCTTCACGTTCATCTCAGAATGCTGCTGATGAACCTTTACCGGAGCAAGACGTGAAGGAATTTCCCTGTCAGTACTGTGACAGGAAATTCTCTACTTATCAGGCTCTTGGAGGACACCAAAACGGTCATAAGCGTGTCCGGGTCTTCAAAAAACAGGAAGACAAAAGGAAAGAGATAGATTTACTCCGAAGGATCGTAGCTGCTAATCAACCATACCCTCATCCATTTTCAAGTCCTACTCCCTATCAAGGGCATCCAAATTTCCGTAGTGCTAACTTGCACAACCCAGTTGGCGCCCATGTGAACAATACCTTGCCTTCTTGGCTTGGTTCACCTTCTGGTGGCTATGGAGGGATGCATATGCCAAATTCACCCCTCACAACACCACAATTTGGGATGACTAATTTTTGGCGTGTAGGTCAAAATGCTGAATTGCCAATTCCACAAAAGTCAAACACTTCGGGTTCGGGACTTTTTGCTCAAGCTAATCAAACTCCTCGATTGGCTGAGGGTGCAGAGAGAACTTTCATCGATCAAATTCATTCTCATGCTCTTCCATTATTATCTCGTGATCCTACTGGAGAAGGCATAATTCAGGCAAATCCCAATGTGCCCTCATCATCAACTCAATCACCCTCAGAGGAACCAACTCAATCAACCTCGGAGAAACTCAACTTGAATCTCCCCCTTTAA

Protein sequence

>MsaT000756.1

MAERISIGNAGDKNLKRKMMEEQEPESKSEKEPKGIPPTSESDVEDLLTLRLGFRPMPTSKALEKGEPSRSSQNAADEPLPEQDVKEFPCQYCDRKFSTYQALGGHQNGHKRVRVFKKQEDKRKEIDLLRRIVAANQPYPHPFSSPTPYQGHPNFRSANLHNPVGAHVNNTLPSWLGSPSGGYGGMHMPNSPLTTPQFGMTNFWRVGQNAELPIPQKSNTSGSGLFAQANQTPRLAEGAERTFIDQIHSHALPLLSRDPTGEGIIQANPNVPSSSTQSPSEEPTQSTSEKLNLNLPL*