Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG000822 | XP_039687740.1 | 95.205 | 584 | 25 | 2 | 1 | 581 | 76 | 659 | 0.0 | 1094 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG000822 | sp|C0LGU0|PRK1_ARATH | 47.119 | 590 | 275 | 9 | 3 | 567 | 85 | 662 | 5.58e-178 | 521 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG000822 | tr|G7I573|G7I573_MEDTR | 95.205 | 584 | 25 | 2 | 1 | 581 | 76 | 659 | 0.0 | 1094 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsaG000009 | MsaG000822 | 0.866880 | 4.148408e-65 | 4.228395e-62 |
| MsaG000116 | MsaG000822 | 0.807060 | 1.031688e-49 | 1.641623e-47 |
| MsaG000124 | MsaG000822 | 0.817315 | 6.114590e-52 | 1.253971e-49 |
| MsaG000137 | MsaG000822 | 0.821460 | 7.013982e-53 | 1.603358e-50 |
| MsaG000138 | MsaG000822 | 0.866157 | 7.021307e-65 | 6.948050e-62 |
| MsaG000161 | MsaG000822 | 0.802734 | 8.193141e-49 | 1.178654e-46 |
| MsaG000167 | MsaG000822 | 0.813744 | 3.780151e-51 | 7.080341e-49 |
| MsaG000253 | MsaG000822 | 0.802822 | 7.860877e-49 | 1.133111e-46 |
| MsaG000260 | MsaG000822 | 0.844296 | 1.543613e-58 | 6.911519e-56 |
| MsaG000276 | MsaG000822 | 0.829347 | 9.719465e-55 | 2.760604e-52 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG000822 | MtrunA17_Chr1g0147701 | 95.205 | 584 | 25 | 2 | 1 | 581 | 76 | 659 | 0.0 | 1094 |
| MsaG000822 | MtrunA17_Chr8g0379571 | 63.553 | 546 | 185 | 6 | 1 | 540 | 122 | 659 | 0.0 | 657 |
| MsaG000822 | MtrunA17_Chr8g0388991 | 47.653 | 554 | 258 | 5 | 1 | 540 | 85 | 620 | 4.60e-175 | 509 |
| MsaG000822 | MtrunA17_Chr2g0334251 | 45.578 | 588 | 278 | 9 | 1 | 555 | 96 | 674 | 3.78e-168 | 493 |
| MsaG000822 | MtrunA17_Chr4g0070291 | 40.575 | 557 | 307 | 6 | 1 | 537 | 74 | 626 | 3.29e-136 | 410 |
| MsaG000822 | MtrunA17_Chr8g0344471 | 38.786 | 593 | 307 | 8 | 1 | 539 | 83 | 673 | 1.07e-128 | 394 |
| MsaG000822 | MtrunA17_Chr6g0488771 | 45.882 | 425 | 197 | 6 | 139 | 544 | 5 | 415 | 7.26e-123 | 369 |
| MsaG000822 | MtrunA17_Chr3g0128551 | 35.135 | 555 | 342 | 9 | 1 | 547 | 81 | 625 | 1.50e-95 | 305 |
| MsaG000822 | MtrunA17_Chr1g0209521 | 35.305 | 558 | 329 | 9 | 9 | 537 | 84 | 638 | 2.26e-94 | 303 |
| MsaG000822 | MtrunA17_Chr8g0387791 | 35.753 | 551 | 320 | 13 | 1 | 540 | 72 | 599 | 9.15e-94 | 300 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG000822 | AT5G35390.1 | 47.119 | 590 | 275 | 9 | 3 | 567 | 85 | 662 | 5.67e-179 | 521 |
| MsaG000822 | AT4G31250.1 | 45.663 | 611 | 268 | 10 | 1 | 566 | 84 | 675 | 1.58e-175 | 513 |
| MsaG000822 | AT2G07040.1 | 46.441 | 590 | 274 | 10 | 3 | 567 | 75 | 647 | 4.86e-167 | 490 |
| MsaG000822 | AT3G20190.1 | 47.160 | 581 | 287 | 9 | 1 | 568 | 104 | 677 | 3.94e-164 | 484 |
| MsaG000822 | AT1G50610.1 | 46.193 | 591 | 291 | 7 | 1 | 568 | 98 | 684 | 1.78e-160 | 474 |
| MsaG000822 | AT3G42880.1 | 37.500 | 568 | 311 | 6 | 1 | 536 | 76 | 631 | 1.01e-125 | 384 |
| MsaG000822 | AT5G20690.1 | 36.535 | 583 | 319 | 11 | 1 | 537 | 81 | 658 | 1.10e-120 | 371 |
| MsaG000822 | AT3G20190.2 | 45.946 | 444 | 220 | 9 | 138 | 568 | 52 | 488 | 6.14e-116 | 353 |
| MsaG000822 | AT2G26730.1 | 38.624 | 567 | 322 | 12 | 2 | 551 | 77 | 634 | 1.15e-104 | 330 |
| MsaG000822 | AT1G72460.1 | 37.522 | 565 | 326 | 10 | 1 | 540 | 75 | 637 | 1.29e-100 | 319 |
Find 106 sgRNAs with CRISPR-Local
Find sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAACAGACATTTGGTGTCT+TGG | 0.382595 | 1:-11266567 | MsaT000822.1:CDS |
| AAAATCACCAGCTAAATCAA+TGG | 0.491609 | 1:+11267681 | None:intergenic |
| AAACCATGCACAACAATACA+TGG | 0.536697 | 1:-11266637 | MsaT000822.1:CDS |
| AAATTAGAGAATATGGGACT+AGG | 0.567285 | 1:-11268520 | None:intergenic |
| AAGAAACTTGTGAAGTTAAG+GGG | 0.529804 | 1:-11268403 | MsaT000822.1:CDS |
| AAGTCCAAATCCAAAAGTCT+AGG | 0.570232 | 1:+11268247 | None:intergenic |
| AAGTTCCGATTTAGCAACAT+GGG | 0.629871 | 1:-11266478 | MsaT000822.1:CDS |
| AATATAACGGGAACAAGAAA+TGG | 0.433714 | 1:-11266408 | MsaT000822.1:CDS |
| AATCTCTTAACCACCACAGT+TGG | 0.602185 | 1:+11267517 | None:intergenic |
| AATGGAGTGTTGAGAGGAGA+TGG | 0.509536 | 1:-11266338 | MsaT000822.1:CDS |
CRISPR-GE
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr1 | gene | 11266164 | 11268530 | 11266164 | ID=MsaG000822 |
| Chr1 | mRNA | 11266164 | 11268530 | 11266164 | ID=MsaT000822.1;Parent=MsaG000822 |
| Chr1 | exon | 11266164 | 11266855 | 11266164 | ID=MsaT000822.1.exon3;Parent=MsaT000822.1 |
| Chr1 | CDS | 11266164 | 11266855 | 11266164 | ID=cds.MsaT000822.1;Parent=MsaT000822.1 |
| Chr1 | exon | 11267347 | 11267982 | 11267347 | ID=MsaT000822.1.exon2;Parent=MsaT000822.1 |
| Chr1 | CDS | 11267347 | 11267982 | 11267347 | ID=cds.MsaT000822.1;Parent=MsaT000822.1 |
| Chr1 | exon | 11268113 | 11268530 | 11268113 | ID=MsaT000822.1.exon1;Parent=MsaT000822.1 |
| Chr1 | CDS | 11268113 | 11268530 | 11268113 | ID=cds.MsaT000822.1;Parent=MsaT000822.1 |
| Gene Sequence |
>MsaT000822.1 ATGGGACTAGGTGGGAAAATTGATGTAGACACATTGTTGGAATTGACTGATTTGGTTAGTTTTAGTGTCAATAACAACACATTTGAGGGTCCAATGCCTGAATTCAAGAAACTTGTGAAGTTAAGGGGATTGTTTTTGTCTGATAATAAATTCTATGGTGAAATTTTAGATGATAGTTTTGAGGGTATGGGAAATCTTAAAAGGGTATTTTTGGCAGGAAATGGGTTCAATGGTCATATTCCTCTTTCACTTGCTCAATTGCCTAGACTTTTGGATTTGGACTTGCATGGAAATAGTTTTGGAGGTAACATACCAGAATTTCAACAAAATGGTTTTAGAAAATTTGACTTGTCTAACAATCAATTGGAGGGTCCAATACCAAATAGCTTAAGCAATGAGCCTTCAACTTCATTTTCTGCCAACAAAGGCCTATGTGGAAAACCTTTGAACAATCCTTGCAACACACCCCCAACAAAATCTATTGTGCAACCTAATTCAGATCCTTCAACTCAAGGAAAAGGAAAGAACAAAAAAATCCTTATAGTTGTCATTGTAGTGGTAGCTATGGTTGTTTTAGCTTCAATATTAGCACTTTTGTTCATCCAAAGCCGCCGGAGACGAAGATCAGAACAAGACCAGCCCATATTAGGCCTACAACTGAATTCTGAAAGCAGTCCAAGTGTCAAGGAGACAAAATCCATTGATTTAGCTGGTGATTTTAGTAAAGGCGAAAATGGAGAATTGAATTTTGTTAGGGAAGATAAAGGTGGTTTTGAGTTGCAAGATCTTCTTAGGGCTTCAGCTGAAGTGCTTGGAAGTGGAAGTTTTGGATCAACTTATAAAGCAATTGTTTTGAATGGACCAACTGTGGTGGTTAAGAGATTTAGGCACATGAATAATGTTGGGAAACAAGAGTTTTTTGAACACATGAAAAAGCTTGGAAGTTTGACACATCCTAATCTACTCCCTCTTATTGCATTCTACTACAAAAAAGAAGAGAAGTTTTTGGTTTATGATTTTGGTGAAAATGGTAGCTTGGCTAGTCATCTACATGGTAGAAACGGCATTGTACTAAACTGGTCAACTCGTCTAAAAATCATAAAAGGAGTAGCAAGAGGCTTAGCACACCTCTACAAAGAATTCCCTACACAAAACCTACCACATGGTCATCTAAAATCCTCCAATGTAATGCTAAACAATTCATTTGACCCACTTTTAACTGAGTATGGACTTGTACCTATAACAAACAAAAACCATGCACAACAATACATGGCATCCTATAAATCACCGGAAGTCACACACTTCAATAGACCAAATGAAAAAACAGACATTTGGTGTCTTGGAATCCTCATACTAGAATTACTAACAGGAAAATTTCCAGCAAATTATTTAAGACATGGAAAAGGCGGAAGTTCCGATTTAGCAACATGGGTGAATTCAGTTGTAAGGGAAGAATGGACTGGTGAAGTGTTTGATAAGAATATAACGGGAACAAGAAATGGTGAAGGTGAGATGTTGAAGCTTTTGAGGATTGGAATGTATTGTTGTGAATGGAGTGTTGAGAGGAGATGGGATTGGAAAGAAGCTTTGGATAAGATTGAAGAGCTTAAGGAGAATGATGGTGAAGATGAATCTTTTTCTTATGTTAGTGAAGGTGATTTGTATTCAAGAGGTGCAACTGAAGATGAATTTTCTTTTTCTGTTACTGATTCACAGGCTGATAAGTTTGGAAATGTTACAGGCTAG |
| Protein sequence |
>MsaT000822.1 MGLGGKIDVDTLLELTDLVSFSVNNNTFEGPMPEFKKLVKLRGLFLSDNKFYGEILDDSFEGMGNLKRVFLAGNGFNGHIPLSLAQLPRLLDLDLHGNSFGGNIPEFQQNGFRKFDLSNNQLEGPIPNSLSNEPSTSFSANKGLCGKPLNNPCNTPPTKSIVQPNSDPSTQGKGKNKKILIVVIVVVAMVVLASILALLFIQSRRRRRSEQDQPILGLQLNSESSPSVKETKSIDLAGDFSKGENGELNFVREDKGGFELQDLLRASAEVLGSGSFGSTYKAIVLNGPTVVVKRFRHMNNVGKQEFFEHMKKLGSLTHPNLLPLIAFYYKKEEKFLVYDFGENGSLASHLHGRNGIVLNWSTRLKIIKGVARGLAHLYKEFPTQNLPHGHLKSSNVMLNNSFDPLLTEYGLVPITNKNHAQQYMASYKSPEVTHFNRPNEKTDIWCLGILILELLTGKFPANYLRHGKGGSSDLATWVNSVVREEWTGEVFDKNITGTRNGEGEMLKLLRIGMYCCEWSVERRWDWKEALDKIEELKENDGEDESFSYVSEGDLYSRGATEDEFSFSVTDSQADKFGNVTG* |
