Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG000848 | GAU35997.1 | 60.938 | 64 | 25 | 0 | 1 | 64 | 1 | 64 | 2.95e-15 | 76.6 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG000848 | tr|A0A2Z6P0N6|A0A2Z6P0N6_TRISU | 60.938 | 64 | 25 | 0 | 1 | 64 | 1 | 64 | 1.41e-15 | 76.6 |
| Gene ID | Type | Classification |
|---|---|---|
| MsaG000848 | TF | MADS-M-type |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG000848 | MtrunA17_Chr8g0351751 | 65.574 | 61 | 21 | 0 | 1 | 61 | 1 | 61 | 1.88e-18 | 75.5 |
| MsaG000848 | MtrunA17_Chr3g0107331 | 52.459 | 61 | 29 | 0 | 4 | 64 | 3 | 63 | 7.36e-16 | 67.0 |
| MsaG000848 | MtrunA17_Chr3g0107381 | 55.738 | 61 | 27 | 0 | 1 | 61 | 1 | 61 | 1.23e-15 | 67.4 |
| MsaG000848 | MtrunA17_Chr4g0013041 | 63.934 | 61 | 20 | 1 | 1 | 61 | 1 | 59 | 1.63e-15 | 67.0 |
| MsaG000848 | MtrunA17_Chr3g0107351 | 51.667 | 60 | 29 | 0 | 5 | 64 | 4 | 63 | 2.03e-14 | 63.2 |
| MsaG000848 | MtrunA17_Chr4g0014321 | 58.333 | 60 | 25 | 0 | 1 | 60 | 1 | 60 | 3.57e-14 | 63.9 |
| MsaG000848 | MtrunA17_Chr3g0107341 | 53.333 | 60 | 28 | 0 | 5 | 64 | 4 | 63 | 4.38e-14 | 62.4 |
| MsaG000848 | MtrunA17_Chr3g0107361 | 50.000 | 60 | 30 | 0 | 5 | 64 | 4 | 63 | 2.07e-13 | 60.5 |
| MsaG000848 | MtrunA17_Chr2g0296831 | 62.500 | 64 | 24 | 0 | 1 | 64 | 1 | 64 | 3.56e-13 | 59.3 |
| MsaG000848 | MtrunA17_Chr2g0283941 | 51.613 | 62 | 29 | 1 | 1 | 62 | 1 | 61 | 6.58e-12 | 57.8 |
| MsaG000848 | MtrunA17_Chr3g0110131 | 60.656 | 61 | 24 | 0 | 1 | 61 | 1 | 61 | 7.85e-12 | 57.8 |
| MsaG000848 | MtrunA17_Chr4g0014031 | 62.295 | 61 | 23 | 0 | 1 | 61 | 1 | 61 | 1.28e-11 | 57.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG000848 | AT5G27960.1 | 48.276 | 58 | 30 | 0 | 3 | 60 | 1 | 58 | 2.80e-11 | 57.0 |
Find 13 sgRNAs with CRISPR-Local
Find sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CACAAGCTTCATTTCTATAA+AGG | 0.239694 | 1:+11530507 | None:intergenic |
| ACAAGCTTCATTTCTATAAA+GGG | 0.333929 | 1:+11530508 | None:intergenic |
| GAAAAGCAACATACAAGAAA+AGG | 0.412608 | 1:-11530568 | MsaT000848.1:CDS |
| GAAGAATGATTTATTCGATA+AGG | 0.444049 | 1:-11530546 | MsaT000848.1:CDS |
| TCATTTCTATAAAGGGTGCT+TGG | 0.483092 | 1:+11530515 | None:intergenic |
| CCTGGTGATGGACAAATCTC+AGG | 0.489413 | 1:+11530455 | None:intergenic |
| AGAGTATTCTAGACTTCCCC+TGG | 0.496161 | 1:+11530437 | None:intergenic |
| TTCTAGACTTCCCCTGGTGA+TGG | 0.516035 | 1:+11530443 | None:intergenic |
| CCTGAGATTTGTCCATCACC+AGG | 0.575929 | 1:-11530455 | MsaT000848.1:CDS |
| TCTCAGGTTGTGGTTCATAA+CGG | 0.588432 | 1:+11530471 | None:intergenic |
| GATGGACAAATCTCAGGTTG+TGG | 0.612489 | 1:+11530461 | None:intergenic |
| CTGAGATTTGTCCATCACCA+GGG | 0.642186 | 1:-11530454 | MsaT000848.1:CDS |
| TGAGATTTGTCCATCACCAG+GGG | 0.698659 | 1:-11530453 | MsaT000848.1:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr1 | gene | 11530445 | 11530639 | 11530445 | ID=MsaG000848 |
| Chr1 | mRNA | 11530445 | 11530639 | 11530445 | ID=MsaT000848.1;Parent=MsaG000848 |
| Chr1 | exon | 11530445 | 11530639 | 11530445 | ID=MsaT000848.1.exon1;Parent=MsaT000848.1 |
| Chr1 | CDS | 11530445 | 11530639 | 11530445 | ID=cds.MsaT000848.1;Parent=MsaT000848.1 |
| Gene Sequence |
| Protein sequence |
