AlfalfaGEDB | MsaG000851

M. sativa ssp. caerulea / MsaG000851

KEGG: -
GO: GO:0001678 GO:0003674 GO:0003700 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0008150 GO:0009743 GO:0009746 GO:0009749 GO:0009889 GO:0009987 GO:0010033 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0019725 GO:0031323 GO:0031326 GO:0033500 GO:0034284 GO:0042221 GO:0042592 GO:0042593 GO:0042802 GO:0042803 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0046982 GO:0046983 GO:0048878 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051252 GO:0051716 GO:0055082 GO:0060255 GO:0065007 GO:0065008 GO:0070887 GO:0071310 GO:0071322 GO:0071326 GO:0071331 GO:0071333 GO:0080090 GO:0140110 GO:1901700 GO:1901701 GO:1903506 GO:2000112 GO:2001141
PFAM: PF00170.24

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG000851	XP_003588595.1	96.154	286	8	2	1	284	54	338	0.0	554

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG000851	sp\|Q9FUD3\|BZIP9_ARATH	48.425	254	100	7	39	284	46	276	2.46e-60	196

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG000851	tr\|G7I545\|G7I545_MEDTR	96.154	286	8	2	1	284	54	338	0.0	554

TFs/TRs:

Gene ID	Type	Classification
MsaG000851	TF	bZIP

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG000851	MtrunA17_Chr1g0147491	96.154	286	8	2	1	284	54	338	0.0	554
MsaG000851	MtrunA17_Chr3g0139301	62.105	285	75	14	10	284	33	294	9.48e-98	289
MsaG000851	MtrunA17_Chr1g0190761	58.065	124	48	2	86	205	177	300	1.41e-27	110
MsaG000851	MtrunA17_Chr7g0274141	52.381	147	54	4	64	205	150	285	2.64e-26	106
MsaG000851	MtrunA17_Chr3g0139571	78.378	74	16	0	116	189	1	74	3.56e-26	98.6
MsaG000851	MtrunA17_Chr3g0144941	41.558	77	45	0	114	190	27	103	8.42e-12	62.0
MsaG000851	MtrunA17_Chr1g0155551	29.747	158	96	2	89	246	3	145	9.10e-12	62.0
MsaG000851	MtrunA17_Chr8g0341311	43.478	92	47	1	98	189	60	146	2.23e-11	61.6
MsaG000851	MtrunA17_Chr6g0456061	40.385	104	56	3	102	204	77	175	2.27e-11	62.0
MsaG000851	MtrunA17_Chr8g0378901	34.884	86	56	0	111	196	29	114	2.71e-11	60.8
MsaG000851	MtrunA17_Chr7g0225661	43.478	92	47	1	98	189	70	156	2.86e-11	61.6

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG000851	AT5G24800.1	48.425	254	100	7	39	284	46	276	2.50e-61	196
MsaG000851	AT5G28770.1	58.333	108	43	1	101	206	130	237	4.95e-25	102
MsaG000851	AT5G28770.2	58.333	108	43	1	101	206	137	244	5.49e-25	102
MsaG000851	AT5G28770.3	61.111	90	35	0	101	190	137	226	7.15e-21	89.7
MsaG000851	AT4G02640.4	53.097	113	51	1	90	200	188	300	5.47e-16	77.8
MsaG000851	AT4G02640.3	53.097	113	51	1	90	200	194	306	5.50e-16	77.8
MsaG000851	AT4G02640.2	53.097	113	51	1	90	200	194	306	6.42e-16	77.8
MsaG000851	AT4G02640.1	53.097	113	51	1	90	200	188	300	6.84e-16	77.4
MsaG000851	AT3G54620.1	51.136	88	43	0	113	200	227	314	1.77e-13	70.5
MsaG000851	AT1G75390.1	39.759	83	50	0	114	196	38	120	5.96e-12	63.5
MsaG000851	AT2G18160.1	36.190	105	57	1	92	196	16	110	1.48e-11	62.4
MsaG000851	AT3G30530.1	39.773	88	53	0	102	189	66	153	3.13e-11	61.2
MsaG000851	AT1G75390.2	44.776	67	37	0	114	180	38	104	8.27e-11	58.9

Find 61 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AGTTCTTGGGAGTGAGGTTT+TGG	0.180329	1:-11555786	None:intergenic
TGTTCAATTGACTCTGATTT+TGG	0.206784	1:-11558804	None:intergenic
CATCTGTAAGCTGCTTAAAT+AGG	0.310185	1:-11558261	None:intergenic
TTTGGCAGACCTTGAGGTTC+AGG	0.310946	1:+11558079	MsaT000851.1:CDS
CTAAGACGCTTCATATCAAC+AGG	0.337570	1:-11556797	None:intergenic
TGCAGTGATGGTGGAGTTCT+TGG	0.341951	1:-11555800	None:intergenic
TGAGCTCTTTGACTAGCTTA+TGG	0.345147	1:+11559007	MsaT000851.1:CDS
AAGAAGAAAACAAGCACATT+TGG	0.358483	1:+11558061	MsaT000851.1:CDS
TGTTGTTGTAGTCACTCCTT+TGG	0.367802	1:-11556708	None:intergenic
GGTGAAGTTAGCAGAGGATA+TGG	0.388906	1:+11558741	MsaT000851.1:intron
AGTATTACCGGTGGCAGAAA+TGG	0.392731	1:-11555842	None:intergenic
CAGAATTCAGCTATTGGACT+TGG	0.407633	1:+11558928	MsaT000851.1:CDS
AGTGGACAGAATTCAGCTAT+TGG	0.421178	1:+11558922	MsaT000851.1:CDS
TTTCCATGGACGGTGATTGT+AGG	0.427364	1:-11558880	None:intergenic
CATGGAAACGACGCTTCATA+TGG	0.432634	1:+11558895	MsaT000851.1:CDS
CAAACAAGAGTATTATTTGT+TGG	0.433570	1:-11555760	None:intergenic
CACTAGTAATCTCCGTTGTA+TGG	0.434010	1:+11558846	MsaT000851.1:CDS
TGTTAGCATCGCGAAATTGT+TGG	0.440152	1:-11558288	None:intergenic
TTGATATGAAGCGTCTTAGA+AGG	0.440492	1:+11556801	MsaT000851.1:CDS
TTAGGTTGAACAGCTGAGGC+TGG	0.444559	1:+11558228	MsaT000851.1:intron
CATATGAAGCGTCGTTTCCA+TGG	0.454343	1:-11558894	None:intergenic
GACGCTTCATATGGTGTTAG+TGG	0.460901	1:+11558904	MsaT000851.1:CDS
AGCAGAGGATATGGTCTCTA+GGG	0.483549	1:+11558750	MsaT000851.1:CDS
GCAGTGATGGTGGAGTTCTT+GGG	0.488823	1:-11555799	None:intergenic
TGGTGGAGTTCTTGGGAGTG+AGG	0.489497	1:-11555792	None:intergenic
AGATTACTAGTGTTGATTTG+TGG	0.491316	1:-11558835	None:intergenic
ACTGACCAGATTATGAAAAT+CGG	0.504341	1:-11553285	None:intergenic
TAGCAGAGGATATGGTCTCT+AGG	0.512033	1:+11558749	MsaT000851.1:CDS
ACATCGCCGACACTGATGAA+TGG	0.512087	1:-11553236	None:intergenic
AGAAGCTGGTTGTTAAAAGT+AGG	0.514450	1:-11558781	None:intergenic
TGCAGTCATGATTTCAACAA+TGG	0.523773	1:+11558967	MsaT000851.1:CDS
ATTATGAAAATCGGCGGTGT+CGG	0.528802	1:-11553276	None:intergenic
TGTTGAAGCTTTAAGAGCTA+AGG	0.529338	1:+11558336	MsaT000851.1:CDS
AGCTCTTAAAGCTTCAACAT+CGG	0.530694	1:-11558332	None:intergenic
GCTTCATCATCTTCATCAGA+TGG	0.530813	1:-11556749	None:intergenic
CTCAATAGTTGCAGTGATGG+TGG	0.531409	1:-11555809	None:intergenic
ACATGTTGAAAATGAAGTTA+CGG	0.531605	1:-11555731	None:intergenic
TTTCTTAGGTTGAACAGCTG+AGG	0.532387	1:+11558224	MsaT000851.1:intron
TCGCCTACAATCACCGTCCA+TGG	0.536280	1:+11558877	MsaT000851.1:CDS
CAATCATCCATTTCTGCCAC+CGG	0.538002	1:+11555835	MsaT000851.1:CDS
TGAAGCGTCGTTTCCATGGA+CGG	0.541190	1:-11558890	None:intergenic
AGCATCGCGAAATTGTTGGC+TGG	0.553901	1:-11558284	None:intergenic
AGTGTCGGCGATGTTTGCTC+TGG	0.559900	1:+11553245	MsaT000851.1:CDS
CTAGGTTTATTAGCTGACAC+TGG	0.571493	1:-11556671	None:intergenic
AGCACATTTGGCAGACCTTG+AGG	0.572432	1:+11558073	MsaT000851.1:CDS
TCTGATGAAGATGATGAAGC+AGG	0.584800	1:+11556752	MsaT000851.1:CDS
GGGTTTGTACTTTGTTCACA+AGG	0.595616	1:-11556776	None:intergenic
AATTAATTACCTGAACCTCA+AGG	0.595756	1:-11558088	None:intergenic
AGACTCAATAGTTGCAGTGA+TGG	0.603647	1:-11555812	None:intergenic
ATGGATCCATTCATCAGTGT+CGG	0.609480	1:+11553230	MsaT000851.1:CDS
AGTAGAGAAAATCAAACCAA+AGG	0.615439	1:+11556692	MsaT000851.1:CDS
GGAGTGACTACAACAACAAG+TGG	0.619797	1:+11556713	MsaT000851.1:CDS
TTACTAGTGTTGATTTGTGG+CGG	0.621086	1:-11558832	None:intergenic
GCGAAACATGCGCCATACAA+CGG	0.641264	1:-11558858	None:intergenic
AATTTAGGTGAAGTTAGCAG+AGG	0.643088	1:+11558735	MsaT000851.1:intron
TTCATCAGATGGATCACGTG+AGG	0.658342	1:-11556738	None:intergenic
GACCAGATTATGAAAATCGG+CGG	0.666849	1:-11553282	None:intergenic
CTAATCGCGAGTCTGCCAGA+AGG	0.671078	1:+11558033	MsaT000851.1:CDS
AGTGTTGATTTGTGGCGGAG+TGG	0.681459	1:-11558827	None:intergenic
GCAGAGGATATGGTCTCTAG+GGG	0.691454	1:+11558751	MsaT000851.1:CDS
TAAGACGCTTCATATCAACA+GGG	0.724365	1:-11556796	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content

Chromosome	Type	Strat	End	Strand	Name
Chr1	gene	11553230	11559035	11553230	ID=MsaG000851
Chr1	mRNA	11553230	11559035	11553230	ID=MsaT000851.1;Parent=MsaG000851
Chr1	exon	11553230	11553301	11553230	ID=MsaT000851.1.exon1;Parent=MsaT000851.1
Chr1	CDS	11553230	11553301	11553230	ID=cds.MsaT000851.1;Parent=MsaT000851.1
Chr1	exon	11555727	11555856	11555727	ID=MsaT000851.1.exon2;Parent=MsaT000851.1
Chr1	CDS	11555727	11555856	11555727	ID=cds.MsaT000851.1;Parent=MsaT000851.1
Chr1	exon	11556663	11556822	11556663	ID=MsaT000851.1.exon3;Parent=MsaT000851.1
Chr1	CDS	11556663	11556822	11556663	ID=cds.MsaT000851.1;Parent=MsaT000851.1
Chr1	exon	11558025	11558100	11558025	ID=MsaT000851.1.exon4;Parent=MsaT000851.1
Chr1	CDS	11558025	11558100	11558025	ID=cds.MsaT000851.1;Parent=MsaT000851.1
Chr1	exon	11558232	11558357	11558232	ID=MsaT000851.1.exon5;Parent=MsaT000851.1
Chr1	CDS	11558232	11558357	11558232	ID=cds.MsaT000851.1;Parent=MsaT000851.1
Chr1	exon	11558742	11559035	11558742	ID=MsaT000851.1.exon6;Parent=MsaT000851.1
Chr1	CDS	11558742	11559035	11558742	ID=cds.MsaT000851.1;Parent=MsaT000851.1

Gene Sequence

>MsaT000851.1

ATGGATCCATTCATCAGTGTCGGCGATGTTTGCTCTGGTAACATCGCCGACACCGCCGATTTTCATAATCTGGATGCCGTAACTTCATTTTCAACATGTGGTGAACCAACAAATAATACTCTTGTTTGCTCCCAAAACCTCACTCCCAAGAACTCCACCATCACTGCAACTATTGAGTCTCAATCATCCATTTCTGCCACCGTTACAAGTCCAGTGTCAGCTAATAAACCTAGTAGAGAAAATCAAACCAAAGGAGTGACTACAACAACAAGTGGTTCCTCACGTGATCCATCTGATGAAGATGATGAAGCAGGTCCTTGTGAACAAAGTACAAACCCTGTTGATATGAAGCGTCTTAGAAGAAAGGTTTCTAATCGCGAGTCTGCCAGAAGGTCCAGAAGAAGAAAACAAGCACATTTGGCAGACCTTGAGGTTCAGGTTGAACAGCTGAGGCTGGAAAATGCAAGCCTATTTAAGCAGCTTACAGATGCCAGCCAACAATTTCGCGATGCTAACACTAACAACCGAGTGCTAAAATCCGATGTTGAAGCTTTAAGAGCTAAGGTGAAGTTAGCAGAGGATATGGTCTCTAGGGGTACTTTACCTACTTTTAACAACCAGCTTCTCCAAAATCAGAGTCAATTGAACACCACTCCGCCACAAATCAACACTAGTAATCTCCGTTGTATGGCGCATGTTTCGCCTACAATCACCGTCCATGGAAACGACGCTTCATATGGTGTTAGTGGACAGAATTCAGCTATTGGACTTGGAGATTTTGATATATCTTGCAGTCATGATTTCAACAATGGAGTTAATAGTGATGCTGTGAGCTCTTTGACTAGCTTATGGTCATAA

Protein sequence

>MsaT000851.1

MDPFISVGDVCSGNIADTADFHNLDAVTSFSTCGEPTNNTLVCSQNLTPKNSTITATIESQSSISATVTSPVSANKPSRENQTKGVTTTTSGSSRDPSDEDDEAGPCEQSTNPVDMKRLRRKVSNRESARRSRRRKQAHLADLEVQVEQLRLENASLFKQLTDASQQFRDANTNNRVLKSDVEALRAKVKLAEDMVSRGTLPTFNNQLLQNQSQLNTTPPQINTSNLRCMAHVSPTITVHGNDASYGVSGQNSAIGLGDFDISCSHDFNNGVNSDAVSSLTSLWS*