AlfalfaGEDB | MsaG000859

M. sativa ssp. caerulea / MsaG000859

KEGG: -
GO: -
PFAM: PF01152.24

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG000859	QDC33486.1	99.721	359	1	0	253	611	3	361	0.0	753

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG000859	sp\|Q67XG0\|GLB3_ARATH	79.054	148	30	1	98	245	1	147	6.84e-78	247

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG000859	tr\|A0A4Y5ULN1\|A0A4Y5ULN1_MEDSA	99.721	359	1	0	253	611	3	361	0.0	753

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TFs/TRs:

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Gene ID	Type	Classification
MsaG000859	TF	NAC

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG000859	MtrunA17_Chr1g0147421	97.500	360	7	2	253	611	3	361	0.0	729
MsaG000859	MtrunA17_Chr3g0139331	59.780	363	111	14	267	611	1	346	3.37e-115	347
MsaG000859	MtrunA17_Chr1g0147401	94.595	148	8	0	98	245	1	148	2.08e-99	300
MsaG000859	MtrunA17_Chr6g0484441	75.625	160	35	3	274	433	2	157	2.71e-78	251
MsaG000859	MtrunA17_Chr3g0139391	77.703	148	32	1	98	245	1	147	3.44e-78	245
MsaG000859	MtrunA17_Chr2g0316971	72.903	155	38	2	277	428	23	176	1.58e-76	248
MsaG000859	MtrunA17_Chr4g0060931	65.882	170	52	3	267	433	7	173	3.19e-73	239
MsaG000859	MtrunA17_Chr3g0091921	65.476	168	51	4	270	433	8	172	1.51e-71	233
MsaG000859	MtrunA17_Chr6g0461661	62.941	170	59	2	277	443	18	186	6.21e-70	231
MsaG000859	MtrunA17_Chr7g0253011	64.535	172	54	4	277	443	17	186	2.72e-69	229

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG000859	AT2G24430.2	51.966	356	102	9	271	608	10	314	5.06e-101	310
MsaG000859	AT2G24430.1	51.966	356	102	9	271	608	10	314	5.06e-101	310
MsaG000859	AT3G18400.1	73.750	160	39	2	274	433	2	158	4.96e-79	253
MsaG000859	AT4G32690.1	79.054	148	30	1	98	245	1	147	6.96e-79	247
MsaG000859	AT5G53950.1	72.152	158	40	2	277	431	17	173	8.04e-76	246
MsaG000859	AT5G61430.1	67.647	170	49	3	272	438	11	177	2.07e-73	239
MsaG000859	AT3G15170.1	69.565	161	45	2	272	429	15	174	9.06e-73	236
MsaG000859	AT5G07680.2	68.485	165	46	3	277	438	3	164	1.01e-72	236
MsaG000859	AT5G07680.1	64.641	181	54	4	265	438	1	178	1.39e-72	236
MsaG000859	AT1G76420.1	64.671	167	58	1	266	432	11	176	1.13e-70	231

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Find 156 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGAACGAGGGAGATTATC+AGG	0.345952	1:+11669706	None:intergenic
AAAGAGGAAACCCTACCTCC+CGG	0.588423	1:-11657044	MsaT000859.1:CDS
AAATAAAGTGATCAGTAAGA+AGG	0.577405	1:+11654422	None:intergenic
AAATCGAAATCCGGGAGGTA+GGG	0.547523	1:+11657034	None:intergenic
AAATGCTGCATCATTGTTGG+TGG	0.560573	1:+11654851	None:intergenic
AAATGGGAATATTGATGCAT+TGG	0.541252	1:+11654749	None:intergenic
AAGAAGTTGTTAGCAAAGAA+AGG	0.487064	1:-11657097	MsaT000859.1:CDS
AATAACTTAGGGTTTGGTGG+TGG	0.530200	1:-11654539	MsaT000859.1:CDS
AATAATAACTTAGGGTTTGG+TGG	0.433273	1:-11654542	MsaT000859.1:CDS
AATAATAATAACTTAGGGTT+TGG	0.365351	1:-11654545	MsaT000859.1:CDS

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CRISPR-GE

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badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
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Chromosome	Type	Strat	End	Strand	Name
Chr1	gene	11654433	11669974	11654433	ID=MsaG000859
Chr1	mRNA	11654433	11669974	11654433	ID=MsaT000859.1;Parent=MsaG000859
Chr1	exon	11654433	11655023	11654433	ID=MsaT000859.1.exon8;Parent=MsaT000859.1
Chr1	CDS	11654433	11655023	11654433	ID=cds.MsaT000859.1;Parent=MsaT000859.1
Chr1	exon	11656056	11656327	11656056	ID=MsaT000859.1.exon7;Parent=MsaT000859.1
Chr1	CDS	11656056	11656327	11656056	ID=cds.MsaT000859.1;Parent=MsaT000859.1
Chr1	exon	11656910	11657126	11656910	ID=MsaT000859.1.exon6;Parent=MsaT000859.1
Chr1	CDS	11656910	11657126	11656910	ID=cds.MsaT000859.1;Parent=MsaT000859.1
Chr1	exon	11660393	11660441	11660393	ID=MsaT000859.1.exon5;Parent=MsaT000859.1
Chr1	CDS	11660393	11660441	11660393	ID=cds.MsaT000859.1;Parent=MsaT000859.1
Chr1	exon	11665263	11665407	11665263	ID=MsaT000859.1.exon4;Parent=MsaT000859.1
Chr1	CDS	11665263	11665407	11665263	ID=cds.MsaT000859.1;Parent=MsaT000859.1
Chr1	exon	11667728	11667861	11667728	ID=MsaT000859.1.exon3;Parent=MsaT000859.1
Chr1	CDS	11667728	11667861	11667728	ID=cds.MsaT000859.1;Parent=MsaT000859.1
Chr1	exon	11667950	11668099	11667950	ID=MsaT000859.1.exon2;Parent=MsaT000859.1
Chr1	CDS	11667950	11668099	11667950	ID=cds.MsaT000859.1;Parent=MsaT000859.1
Chr1	exon	11669697	11669974	11669697	ID=MsaT000859.1.exon1;Parent=MsaT000859.1
Chr1	CDS	11669697	11669974	11669697	ID=cds.MsaT000859.1;Parent=MsaT000859.1

Gene Sequence

>MsaT000859.1

ATGGATTTGAAGAAGCAAAGATTTGGTTCAAGCAATTCAACAACTCAAACCATAAGATTAGGTAGAAGCTACACAAAACCACACTATCAAAGCTACCATGATGGGACAAAACCAGTGTGGCAAAAAATTTGGAAAAAACTTAAAAGAGACAAGAAGAAAATCTTTGGCTCAACTCCAACAATAGAAGGTATTTATGATGAAGAATCATACTCAATGAACTTTGATAAAGGAAGTGGGTGGATGGAGCCTGATAATCTCCCTCGTTCTTTCTCTTCTAGTGTCGTTTTAACGATGCAGAGTCTTCAACAGAAAGCTTCAGAATGGAGTGGAGTTTCTACAGATGAAGCTTTCTCCATCGATGAAACCAATCTCTTCCAGAAACTCGGTCTTCAAACCTTCATTAACCTCTCCACCAATTTCTACAATAGGGTTTATGATGATGAAGAAGAAGAATGGTTTCGTTCCATATTTGCGAATTCTAAGAAAGAAGAGGCTATTCAGAATCAGTATGAATTCTTTGTGCAAAGAATGGGAGGGCCTCCGCTTTTTTCTAAGAGAAGAGGTCATCCTGCTCTAATTGGTCGACATCGACCATTTCCAGTCACACATCAAGCTGCGGAGAGGTGGTTACATCACATGCAACTAGCATTGGACACTACTCCAGATATAGATGATGACTCAAAGATCAAAATGATGAACTTCTTCAGGCACATCACTATTCACCTCCTTTGTGGAGGGTCGCCGCCTCCCTCCGCGAAGGACAAAGAAGTTGTTAGCAAAGAAAGGAAGGATTTGGAAATGGAAGGAACACGTGAGAAAGAGGAAACCCTACCTCCCGGATTTCGATTTCATCCTACCGATGAAGAACTCATCACTTACTATCTTGTAAGCAAGATTTCAGATTCTGTAAATTTTACATGCAAAGCAATAGGTGATGTAGATCTCAACAAATGCGAGCCATGGGAGCTTCCAGGGAAGGCCAAGATGGGTACAAAAGAATGGTATTTCTTCAGTCTAAGAGATCGTAAATATCCAACAGGTGTGAGAACTAATAGAGCAACAAACACTGGATATTGGAAAACCACAGGGAAAGACAAGGAGATTTTCAACAGTGTTACATCAGAATTGGTTGGTATGAAGAAAACTTTGGTTTTTTACAAAGGTCGTGCTCCTAGAGGAGAGAAAACCAACTGGGTCATGCATGAATATCGTATCCATTCCAAATCCAGTATAAGAACCAACAAGCAGGATGAATGGGTGGTAAGCAGAGTATTCCGCAAGAGTGCAGGTGCAAAGAAGTACCCTTCTTCTTCTTCAAATCTTACAAGAGCAGTCATGAATCCTTACCATATCAATCTAGAAGTTGGTCCTCCTAGCTTTAATAATAATATGCCACCACCAACAATGATGCAGCATTTAGGGCTAGATTCTGCTACTCATTTCCAGCTCTATGGAAGGAACTATATCAACACTGGAGAAGTTTTAAGAGCTGGTGTACCAATGCATCAATATTCCCATTTATCATCATCACCCTTAGGAGGAGAAGGATTCACCATTTCAGGGCTTAATTTGAATCTTGGAGGATCCACGTCTTCACAAACTATTTTGAGGCCAATGCCATCAACTACTCATACTATGGCTGCTGATTTCAGCTCCAACATGATGACAGGTGCAAATAGTAATACCCTTGTTGCTGCAGAGAATAATAATAACTTAGGGTTTGGTGGTGGAGATCAAGTGAATAATGCAAACCCTACTCATGGCAATAGGTACAATATGCAGGGTACTATGGACCATTGTGTGGATCTTGATCATTACTGGCCACCTTCTTACTGA

Protein sequence

>MsaT000859.1

MDLKKQRFGSSNSTTQTIRLGRSYTKPHYQSYHDGTKPVWQKIWKKLKRDKKKIFGSTPTIEGIYDEESYSMNFDKGSGWMEPDNLPRSFSSSVVLTMQSLQQKASEWSGVSTDEAFSIDETNLFQKLGLQTFINLSTNFYNRVYDDEEEEWFRSIFANSKKEEAIQNQYEFFVQRMGGPPLFSKRRGHPALIGRHRPFPVTHQAAERWLHHMQLALDTTPDIDDDSKIKMMNFFRHITIHLLCGGSPPPSAKDKEVVSKERKDLEMEGTREKEETLPPGFRFHPTDEELITYYLVSKISDSVNFTCKAIGDVDLNKCEPWELPGKAKMGTKEWYFFSLRDRKYPTGVRTNRATNTGYWKTTGKDKEIFNSVTSELVGMKKTLVFYKGRAPRGEKTNWVMHEYRIHSKSSIRTNKQDEWVVSRVFRKSAGAKKYPSSSSNLTRAVMNPYHINLEVGPPSFNNNMPPPTMMQHLGLDSATHFQLYGRNYINTGEVLRAGVPMHQYSHLSSSPLGGEGFTISGLNLNLGGSTSSQTILRPMPSTTHTMAADFSSNMMTGANSNTLVAAENNNNLGFGGGDQVNNANPTHGNRYNMQGTMDHCVDLDHYWPPSY*