AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG000889


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG000889 MtrunA17_Chr1g0147111 97.976 247 5 0 3 249 36 282 0.0 498
MsaG000889 MtrunA17_Chr3g0126721 73.878 245 56 4 3 245 38 276 8.24e-117 335
MsaG000889 MtrunA17_Chr8g0387241 48.235 85 42 1 161 243 185 269 2.58e-21 90.9
MsaG000889 MtrunA17_Chr5g0397361 45.882 85 42 1 161 245 139 219 1.91e-17 79.0
MsaG000889 MtrunA17_Chr1g0168401 35.172 145 76 4 117 244 221 364 4.36e-17 79.7
MsaG000889 MtrunA17_Chr1g0186171 36.296 135 69 4 126 244 228 361 1.19e-16 78.6
MsaG000889 MtrunA17_Chr2g0327621 33.813 139 76 4 121 247 69 203 1.21e-16 77.0
MsaG000889 MtrunA17_Chr4g0030421 42.857 84 44 2 161 244 1 80 1.46e-16 73.9
MsaG000889 MtrunA17_Chr2g0311591 53.061 49 23 0 195 243 166 214 9.00e-12 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG000889 AT5G53420.1 52.846 246 95 7 1 243 35 262 2.46e-78 237
MsaG000889 AT4G27900.1 51.575 254 91 6 4 244 25 259 1.52e-75 230
MsaG000889 AT4G27900.2 51.575 254 91 6 4 244 25 259 1.52e-75 230
MsaG000889 AT5G53420.2 55.224 201 69 7 46 243 1 183 2.57e-64 199
MsaG000889 AT5G53420.5 55.224 201 69 7 46 243 1 183 2.57e-64 199
MsaG000889 AT5G53420.4 55.224 201 69 7 46 243 1 183 2.57e-64 199
MsaG000889 AT5G53420.3 45.763 177 82 6 1 175 35 199 9.28e-40 137
MsaG000889 AT5G41380.1 46.316 95 45 2 156 244 166 260 5.06e-20 87.4
MsaG000889 AT1G63820.1 41.584 101 40 2 161 244 139 237 5.81e-19 84.3
MsaG000889 AT2G33350.2 39.831 118 47 3 151 244 238 355 3.92e-17 80.5
MsaG000889 AT2G33350.4 39.831 118 47 3 151 244 227 344 5.82e-17 79.7
MsaG000889 AT2G33350.5 39.831 118 47 3 151 244 247 364 6.21e-17 79.7
MsaG000889 AT1G04500.4 64.000 50 18 0 195 244 284 333 1.17e-16 79.0
MsaG000889 AT1G04500.1 64.000 50 18 0 195 244 284 333 1.17e-16 79.0
MsaG000889 AT1G04500.3 64.000 50 18 0 195 244 275 324 1.17e-16 79.0
MsaG000889 AT2G33350.1 64.000 50 18 0 195 244 307 356 4.10e-16 77.4
MsaG000889 AT5G59990.1 59.091 44 18 0 206 249 170 213 8.58e-12 63.5

Find 55 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTCTTCTTATTCCTATATC+TGG 0.246824 1:+12007503 None:intergenic
TTCAGGAAATTTGAGTTCAA+TGG 0.350069 1:-12008146 MsaT000889.1:CDS
GCAAATCTTCCACGGATTCT+TGG 0.350555 1:+12006752 None:intergenic
GATAATAGTACATCAGGAAT+TGG 0.361584 1:+12008181 None:intergenic
CCTGCTTTCCTTGATTTCCC+TGG 0.370726 1:-12008097 MsaT000889.1:CDS
CCGTGGAAGATTTGCGAAAA+CGG 0.381688 1:-12006744 MsaT000889.1:CDS
AAGAAGACAAGGAGAAACTT+TGG 0.387483 1:-12007489 MsaT000889.1:CDS
CATGGTTTCAGTATGCTTGT+AGG 0.397320 1:-12006796 MsaT000889.1:intron
AGGAAAGCAGGCTTCATTGC+AGG 0.402149 1:+12008109 None:intergenic
ACCGCGTTAAAATCAATTCC+AGG 0.422111 1:+12008079 None:intergenic
GAAATTTGAGTTCAATGGAT+TGG 0.431971 1:-12008141 MsaT000889.1:CDS
TTATCAAAGAGTGTCAGTTC+AGG 0.440427 1:-12008163 MsaT000889.1:CDS
GAGCAGGAATCTTGATTTCA+AGG 0.444374 1:+12008239 None:intergenic
CTCTTTGATAATAGTACATC+AGG 0.447091 1:+12008175 None:intergenic
AGAAGACAAGGAGAAACTTT+GGG 0.461172 1:-12007488 MsaT000889.1:CDS
GTGCAGTTGCTGAGAAGAAA+AGG 0.470162 1:+12007552 None:intergenic
AATCAATTCCAGGGAAATCA+AGG 0.470610 1:+12008089 None:intergenic
TGGGGAAGACATGTCGTCGC+AGG 0.471749 1:-12008383 MsaT000889.1:CDS
ATTTAGTGAAGGAGATATAA+AGG 0.474162 1:-12008038 MsaT000889.1:intron
CGGTTCTTCAATAATCGTCT+CGG 0.477444 1:+12008453 None:intergenic
CGAGACGATTATTGAAGAAC+CGG 0.482715 1:-12008452 MsaT000889.1:CDS
TGGCTGCTTGCATTTGATCA+AGG 0.489809 1:+12008422 None:intergenic
GTCGCAGGGTCTAAAATCTT+CGG 0.499847 1:-12008368 MsaT000889.1:CDS
TTGTTTGTATAACAGACTCT+TGG 0.509295 1:-12007615 MsaT000889.1:intron
AGAATTTGATTTATTTGTAG+AGG 0.514069 1:-12008494 MsaT000889.1:CDS
TTCTCGTCAATGTTCAGAGC+AGG 0.527138 1:+12008223 None:intergenic
ATTTGATCAAGGTCCATCTC+CGG 0.536269 1:+12008433 None:intergenic
CCAGGGAAATCAAGGAAAGC+AGG 0.538494 1:+12008097 None:intergenic
GAATTTGATTTATTTGTAGA+GGG 0.539422 1:-12008493 MsaT000889.1:CDS
AGTTCAATGGATTGGATGCA+TGG 0.542974 1:-12008133 MsaT000889.1:CDS
ATTTCAAGGATTTCAGAAAG+AGG 0.543254 1:+12008253 None:intergenic
GCAGCCATGTCAATGATGTC+TGG 0.545025 1:-12008406 MsaT000889.1:CDS
GATTATTGAAGAACCGGAGA+TGG 0.549985 1:-12008446 MsaT000889.1:CDS
GGTTCTTCAATAATCGTCTC+GGG 0.551243 1:+12008454 None:intergenic
CCGCGTTAAAATCAATTCCA+GGG 0.559154 1:+12008080 None:intergenic
AAGGCCTCTCTAGAGTGGAT+TGG 0.563933 1:+12007571 None:intergenic
AAACTTTGGGAGGAAAATCA+AGG 0.564099 1:-12007475 MsaT000889.1:intron
GGGGAAGACATGTCGTCGCA+GGG 0.576526 1:-12008382 MsaT000889.1:CDS
AAGAAAAGGCCTCTCTAGAG+TGG 0.578670 1:+12007566 None:intergenic
CGGAGGAGTTAGATATCAAG+AGG 0.594972 1:-12006724 MsaT000889.1:CDS
CCATGTCAATGATGTCTGGT+GGG 0.605089 1:-12008402 MsaT000889.1:CDS
GTTTCAGTATGCTTGTAGGA+AGG 0.605930 1:-12006792 MsaT000889.1:intron
GTATAACAGACTCTTGGCAA+TGG 0.606169 1:-12007609 MsaT000889.1:intron
GACAGTCAACCAAGAATCCG+TGG 0.616160 1:-12006761 MsaT000889.1:CDS
GGCAATGGTAACATGAGTGT+TGG 0.622717 1:-12007594 MsaT000889.1:CDS
CCCACCAGACATCATTGACA+TGG 0.626711 1:+12008402 None:intergenic
GCCATGTCAATGATGTCTGG+TGG 0.631276 1:-12008403 MsaT000889.1:CDS
GATATAGGAATAAGAAGACA+AGG 0.632233 1:-12007500 MsaT000889.1:CDS
ATGCGAAGAGCATTTAGTGA+AGG 0.636958 1:-12008049 MsaT000889.1:CDS
AGAAAGAGGTGATTCTATAG+CGG 0.645696 1:+12008267 None:intergenic
TTGGCCAATCCACTCTAGAG+AGG 0.651640 1:-12007575 MsaT000889.1:CDS
TGGAAGATTTGCGAAAACGG+AGG 0.654676 1:-12006741 MsaT000889.1:CDS
ACATCGCATATGATTGAACA+AGG 0.660417 1:+12008518 None:intergenic
CATGTCAATGATGTCTGGTG+GGG 0.675165 1:-12008401 MsaT000889.1:CDS
AGACAAGGAGAAACTTTGGG+AGG 0.688525 1:-12007485 MsaT000889.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr1 gene 12006718 12008551 12006718 ID=MsaG000889
Chr1 mRNA 12006718 12008551 12006718 ID=MsaT000889.1;Parent=MsaG000889
Chr1 exon 12006718 12006807 12006718 ID=MsaT000889.1.exon3;Parent=MsaT000889.1
Chr1 CDS 12006718 12006807 12006718 ID=cds.MsaT000889.1;Parent=MsaT000889.1
Chr1 exon 12007476 12007622 12007476 ID=MsaT000889.1.exon2;Parent=MsaT000889.1
Chr1 CDS 12007476 12007622 12007476 ID=cds.MsaT000889.1;Parent=MsaT000889.1
Chr1 exon 12008039 12008551 12008039 ID=MsaT000889.1.exon1;Parent=MsaT000889.1
Chr1 CDS 12008039 12008551 12008039 ID=cds.MsaT000889.1;Parent=MsaT000889.1
Gene Sequence

>MsaT000889.1

ATGCAGAGTGACCTTGTTCAATCATATGCGATGTCAGAATTTGATTTATTTGTAGAGGGAGATCTGTTCAAAGCACCCGAGACGATTATTGAAGAACCGGAGATGGACCTTGATCAAATGCAAGCAGCCATGTCAATGATGTCTGGTGGGGAAGACATGTCGTCGCAGGGTCTAAAATCTTCGGATATTGATGATGTGCTTCAAAGCGAACAACTTTTGAGTGAGGTCTACTATGATTGCAAAAAAGATCTCTTAGAAAAGGCCGCTATAGAATCACCTCTTTCTGAAATCCTTGAAATCAAGATTCCTGCTCTGAACATTGACGAGAATTCGATTCTAGAAAACAAACCAATTCCTGATGTACTATTATCAAAGAGTGTCAGTTCAGGAAATTTGAGTTCAATGGATTGGATGCATGGTCCTGCAATGAAGCCTGCTTTCCTTGATTTCCCTGGAATTGATTTTAACGCGGTTTATGGCATGCGAAGAGCATTTAGTGAAGGAGATATAAAGACTCTTGGCAATGGTAACATGAGTGTTGGCCAATCCACTCTAGAGAGGCCTTTTCTTCTCAGCAACTGCACGAGTGAGCAACGTCAAGAAAAACTCTCCAGATATAGGAATAAGAAGACAAGGAGAAACTTTGGGAGGAAAATCAAGTATGCTTGTAGGAAGGCGCTTGCTGACAGTCAACCAAGAATCCGTGGAAGATTTGCGAAAACGGAGGAGTTAGATATCAAGAGGCAATGA

Protein sequence

>MsaT000889.1

MQSDLVQSYAMSEFDLFVEGDLFKAPETIIEEPEMDLDQMQAAMSMMSGGEDMSSQGLKSSDIDDVLQSEQLLSEVYYDCKKDLLEKAAIESPLSEILEIKIPALNIDENSILENKPIPDVLLSKSVSSGNLSSMDWMHGPAMKPAFLDFPGIDFNAVYGMRRAFSEGDIKTLGNGNMSVGQSTLERPFLLSNCTSEQRQEKLSRYRNKKTRRNFGRKIKYACRKALADSQPRIRGRFAKTEELDIKRQ*