AlfalfaGEDB | MsaG000924

M. sativa ssp. caerulea / MsaG000924

KEGG: -
GO: GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006275 GO:0006325 GO:0006464 GO:0006479 GO:0006807 GO:0006996 GO:0007275 GO:0008150 GO:0008152 GO:0008168 GO:0008170 GO:0008213 GO:0008276 GO:0008757 GO:0009791 GO:0009889 GO:0009900 GO:0009901 GO:0009908 GO:0009987 GO:0010556 GO:0016043 GO:0016278 GO:0016279 GO:0016569 GO:0016570 GO:0016571 GO:0016740 GO:0016741 GO:0018022 GO:0018024 GO:0018193 GO:0018205 GO:0019219 GO:0019222 GO:0019538 GO:0022414 GO:0031323 GO:0031326 GO:0032259 GO:0032501 GO:0032502 GO:0032504 GO:0034968 GO:0036211 GO:0042054 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0046976 GO:0048367 GO:0048437 GO:0048438 GO:0048443 GO:0048466 GO:0048608 GO:0048609 GO:0048653 GO:0048731 GO:0048827 GO:0048856 GO:0050789 GO:0050794 GO:0051052 GO:0051171 GO:0051276 GO:0051726 GO:0060255 GO:0061458 GO:0065007 GO:0070734 GO:0071704 GO:0071840 GO:0080090 GO:0090567 GO:0099402 GO:0140096 GO:1901564 GO:2000112
PFAM: PF00628.32

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG000924	XP_003588480.1	86.885	61	8	0	361	421	186	246	3.40e-24	114
MsaG000924	XP_003588480.1	96.923	325	9	1	1	324	1	325	0.0	649

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG000924	sp\|Q9FNE9\|ATXR6_ARATH	71.096	301	84	3	26	324	33	332	9.69e-151	434
MsaG000924	sp\|Q9FNE9\|ATXR6_ARATH	70.492	61	18	0	361	421	192	252	7.88e-20	93.6

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG000924	tr\|G7I3E6\|G7I3E6_MEDTR	86.885	61	8	0	361	421	186	246	1.62e-24	114
MsaG000924	tr\|G7I3E6\|G7I3E6_MEDTR	96.923	325	9	1	1	324	1	325	0.0	649

TFs/TRs:

Gene ID	Type	Classification
MsaG000924	TR	PHD

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG000924	MtrunA17_Chr1g0146731	96.923	325	9	1	1	324	1	325	0.0	649
MsaG000924	MtrunA17_Chr1g0146731	86.885	61	8	0	361	421	186	246	3.13e-28	114
MsaG000924	MtrunA17_Chr1g0146761	67.278	327	70	7	1	324	51	343	1.60e-143	413
MsaG000924	MtrunA17_Chr1g0146761	70.492	61	18	0	361	421	203	263	1.70e-21	95.1
MsaG000924	MtrunA17_Chr3g0129881	59.344	305	121	2	22	324	70	373	1.55e-120	355
MsaG000924	MtrunA17_Chr3g0129881	52.542	59	28	0	362	420	235	293	2.41e-13	71.2
MsaG000924	MtrunA17_Chr1g0146751	82.031	128	23	0	184	311	69	196	1.68e-71	223
MsaG000924	MtrunA17_Chr1g0146751	75.000	64	16	0	358	421	67	130	1.91e-24	99.8
MsaG000924	MtrunA17_Chr1g0146741	68.902	164	26	2	116	264	27	180	7.08e-67	211
MsaG000924	MtrunA17_Chr1g0146741	61.765	68	16	1	354	421	104	161	1.26e-18	83.6
MsaG000924	MtrunA17_Chr8g0357751	73.786	103	26	1	184	285	14	116	1.77e-46	157
MsaG000924	MtrunA17_Chr8g0357751	67.692	65	21	0	357	421	11	75	5.05e-23	94.7
MsaG000924	MtrunA17_Chr3g0129871	44.444	72	35	1	1	72	1	67	1.25e-15	71.6

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG000924	AT5G24330.1	71.096	301	84	3	26	324	33	332	9.85e-152	434
MsaG000924	AT5G24330.1	70.492	61	18	0	361	421	192	252	8.01e-21	93.6
MsaG000924	AT5G09790.2	58.278	302	121	2	25	324	64	362	4.09e-124	365
MsaG000924	AT5G09790.2	65.574	61	21	0	361	421	223	283	1.09e-18	87.8
MsaG000924	AT5G09790.1	58.472	301	121	2	25	324	64	361	1.41e-123	363
MsaG000924	AT5G09790.1	65.574	61	21	0	361	421	222	282	1.08e-18	87.8

Find 88 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ATGCATACATTTGGGGATTT+TGG	0.088459	1:+12516879	None:intergenic
GCTATATTGCTACGTTTATC+TGG	0.121333	1:+12514606	None:intergenic
CTTGAAGAAATCAATAATTT+TGG	0.167292	1:+12516585	None:intergenic
ATTTGCCGGAGTTGCATTTC+TGG	0.190452	1:+12517022	None:intergenic
AATATAGCACGTTTCATTAA+TGG	0.211173	1:-12514591	MsaT000924.1:CDS
CATTATATCTCACGCATTTC+AGG	0.255009	1:+12514058	None:intergenic
CAACTTAGTTGGGGATTTGC+CGG	0.329908	1:+12517008	None:intergenic
GGGCAAAACCAAGAGGATTT+AGG	0.342107	1:+12516919	None:intergenic
CTCCTCTTCAAATCATCATT+TGG	0.347535	1:+12515909	None:intergenic
CACAAAGAAGCAACTTAGTT+GGG	0.347651	1:+12516998	None:intergenic
GCATCCCTTGGCGCCATTCC+AGG	0.348908	1:+12515810	None:intergenic
TACAACATATTCTGTTGTTA+TGG	0.360029	1:+12511838	None:intergenic
AACAAACATTACCGCAGATA+TGG	0.381910	1:+12513259	None:intergenic
GTCATCATCGTTGAAGGTTG+TGG	0.391049	1:+12517056	None:intergenic
GAGAAAGCGGAGTAGTAGTT+TGG	0.397233	1:-12515970	MsaT000924.1:CDS
GTTGTTTATGATCCTGTAGA+AGG	0.406850	1:-12514871	MsaT000924.1:CDS
TGGGAGCTGGAATTCTTCTT+CGG	0.410162	1:+12517088	None:intergenic
CTTCTGTACCTAAATCCTCT+TGG	0.418430	1:-12516927	MsaT000924.1:CDS
TTGCATCCATATCTTGTACT+AGG	0.421887	1:-12513331	MsaT000924.1:CDS
GAGATATAGCAAAGGGAGAA+AGG	0.428636	1:-12513999	MsaT000924.1:intron
ATTCCTCCACGTTCAAGAAC+AGG	0.452190	1:+12515780	None:intergenic
GAACACCCTAGTACAAGATA+TGG	0.460271	1:+12513325	None:intergenic
TCACAAAGAAGCAACTTAGT+TGG	0.461329	1:+12516997	None:intergenic
AAAGCGGAGTAGTAGTTTGG+TGG	0.462327	1:-12515967	MsaT000924.1:CDS
GGCAGAGTCTAACACTTAGA+AGG	0.467431	1:+12513297	None:intergenic
CGCGTTGACTGCTACTAAAA+CGG	0.476912	1:-12515859	MsaT000924.1:CDS
TAGGTGTGAGGCAGAAGAGA+TGG	0.477113	1:+12516956	None:intergenic
CTATATTGCTACGTTTATCT+GGG	0.481139	1:+12514607	None:intergenic
TGCGTGAGATATAATGTTGA+TGG	0.484685	1:-12514051	MsaT000924.1:CDS
AAACATGTGCAGAAGTATGA+TGG	0.488621	1:-12512026	MsaT000924.1:CDS
ACAATAATAACAGAATATGT+TGG	0.490743	1:-12514726	MsaT000924.1:CDS
CAAGAGGATTTAGGTACAGA+AGG	0.492170	1:+12516928	None:intergenic
TAACTTGTGCAGAAGTATGA+TGG	0.493269	1:-12514920	MsaT000924.1:CDS
ATAAATACTTACCCTTCTAC+AGG	0.495310	1:+12511965	None:intergenic
TCTGCACATGTTTAGAGTCT+CGG	0.497659	1:+12512036	None:intergenic
TGCATCCATATCTTGTACTA+GGG	0.499673	1:-12513330	MsaT000924.1:CDS
TTAGGTACAGAAGGGAGGAT+AGG	0.500711	1:+12516937	None:intergenic
TTCTAATCCTTCACAGTCAC+TGG	0.505311	1:-12514637	MsaT000924.1:CDS
GACGTCGTCATCATCGTTGA+AGG	0.512130	1:+12517050	None:intergenic
GCATCTAAGATTCTGAATCA+TGG	0.512650	1:-12516544	MsaT000924.1:intron
ATAAGTACTTACCCTTCTAC+AGG	0.515050	1:+12514859	None:intergenic
ATTAGAACAGATGGCGTCGT+TGG	0.518768	1:-12515886	MsaT000924.1:CDS
GCAAATAGAGATATAGCAAA+GGG	0.521433	1:-12514006	MsaT000924.1:CDS
CTTTGTGACAATTGCGACAA+CGG	0.522702	1:-12516982	MsaT000924.1:CDS
GATATGGAGGAACACCAACC+AGG	0.527374	1:+12513275	None:intergenic
AATGAGCTTACGTACATGCC+TGG	0.527386	1:-12515828	MsaT000924.1:CDS
CTAAGTGTTAGACTCTGCCC+TGG	0.535963	1:-12513293	MsaT000924.1:CDS
ATTCAAAGAAGAAGAGAAAG+CGG	0.540362	1:-12515983	MsaT000924.1:CDS
TCTTGAACGTGGAGGAATGC+AGG	0.541405	1:-12515775	MsaT000924.1:intron
AAGAATCGGGAATATGATGA+TGG	0.544853	1:-12514687	MsaT000924.1:CDS
AGGAGAATGTCCGCCCCTCA+TGG	0.545962	1:-12511999	MsaT000924.1:CDS
AGGAGAATGTCCGCCCCTCA+TGG	0.545962	1:-12514893	MsaT000924.1:CDS
TGCAGAAGTATGATGGAAAG+AGG	0.546783	1:-12512019	MsaT000924.1:CDS
TGCAGAAGTATGATGGAAAG+AGG	0.546783	1:-12514913	MsaT000924.1:CDS
TGGGGTTGTGAGAGCAGGAA+GGG	0.547853	1:+12516899	None:intergenic
GTGTTAGACTCTGCCCTGGT+TGG	0.549283	1:-12513289	MsaT000924.1:CDS
ACAGGAGAATTAGCATCCCT+TGG	0.558324	1:+12515798	None:intergenic
CATGCCTGGAATGGCGCCAA+GGG	0.564637	1:-12515814	MsaT000924.1:CDS
GATGCAATCACACATTGCAT+TGG	0.567275	1:+12513347	None:intergenic
TGCAAATAGAGATATAGCAA+AGG	0.569319	1:-12514007	MsaT000924.1:CDS
AATTCTCCTGTTCTTGAACG+TGG	0.577379	1:-12515786	MsaT000924.1:CDS
GGAATTCTTCTTCGGCGAAG+TGG	0.581802	1:+12517096	None:intergenic
TCGGCGAAGTGGTGAAGCCA+TGG	0.586776	1:+12517107	None:intergenic
ACATGCCTGGAATGGCGCCA+AGG	0.588883	1:-12515815	MsaT000924.1:CDS
CAGAATCTTAGATGCATCAA+CGG	0.589395	1:+12516552	None:intergenic
TGCCAAATGATGATTTGAAG+AGG	0.592036	1:-12515911	MsaT000924.1:CDS
GAAGAGGAGATTAGAACAGA+TGG	0.593117	1:-12515895	MsaT000924.1:CDS
AAACATTACCGCAGATATGG+AGG	0.600334	1:+12513262	None:intergenic
TGAAAGGATGCATACATTTG+GGG	0.604501	1:+12516872	None:intergenic
GCTTACGTACATGCCTGGAA+TGG	0.609175	1:-12515823	MsaT000924.1:CDS
TTGGGGTTGTGAGAGCAGGA+AGG	0.612150	1:+12516898	None:intergenic
TTGTTTATGATCCTGTAGAA+GGG	0.614616	1:-12514870	MsaT000924.1:intron
ACAAAGAAGCAACTTAGTTG+GGG	0.616689	1:+12516999	None:intergenic
TCTGCACAAGTTAAGAGTCT+CGG	0.617054	1:+12514930	None:intergenic
AAGAGGATTTAGGTACAGAA+GGG	0.621884	1:+12516929	None:intergenic
GTTTGCCAGAAATGCAACTC+CGG	0.639299	1:-12517027	MsaT000924.1:CDS
AGGATTTAGGTACAGAAGGG+AGG	0.647659	1:+12516932	None:intergenic
TCTCCTGTTCTTGAACGTGG+AGG	0.653063	1:-12515783	MsaT000924.1:CDS
CAGAAGGGAGGATAGGTGTG+AGG	0.654698	1:+12516944	None:intergenic
ATATGGAGGAACACCAACCA+GGG	0.658625	1:+12513276	None:intergenic
GGATCATAAACAACCATGAG+GGG	0.675550	1:+12514880	None:intergenic
AGGATCATAAACAACCATGA+GGG	0.678750	1:+12514879	None:intergenic
TCATAAACAACCATGAGGGG+CGG	0.687468	1:+12514883	None:intergenic
TTGGTGTTCCTCCATATCTG+CGG	0.693068	1:-12513270	MsaT000924.1:intron
CAGGATCATAAACAACCATG+AGG	0.703912	1:+12514878	None:intergenic
GCAGGAAGGGCAAAACCAAG+AGG	0.707902	1:+12516912	None:intergenic
AGAGTCTAACACTTAGAAGG+CGG	0.717515	1:+12513300	None:intergenic
CAGATGACCAGTGACTGTGA+AGG	0.718767	1:+12514630	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content

Chromosome	Type	Strat	End	Strand	Name
Chr1	gene	12511811	12517127	12511811	ID=MsaG000924
Chr1	mRNA	12511811	12517127	12511811	ID=MsaT000924.1;Parent=MsaG000924
Chr1	exon	12511811	12511898	12511811	ID=MsaT000924.1.exon9;Parent=MsaT000924.1
Chr1	CDS	12511811	12511898	12511811	ID=cds.MsaT000924.1;Parent=MsaT000924.1
Chr1	exon	12511977	12512077	12511977	ID=MsaT000924.1.exon8;Parent=MsaT000924.1
Chr1	CDS	12511977	12512077	12511977	ID=cds.MsaT000924.1;Parent=MsaT000924.1
Chr1	exon	12513271	12513382	12513271	ID=MsaT000924.1.exon7;Parent=MsaT000924.1
Chr1	CDS	12513271	12513382	12513271	ID=cds.MsaT000924.1;Parent=MsaT000924.1
Chr1	exon	12514000	12514101	12514000	ID=MsaT000924.1.exon6;Parent=MsaT000924.1
Chr1	CDS	12514000	12514101	12514000	ID=cds.MsaT000924.1;Parent=MsaT000924.1
Chr1	exon	12514573	12514785	12514573	ID=MsaT000924.1.exon5;Parent=MsaT000924.1
Chr1	CDS	12514573	12514785	12514573	ID=cds.MsaT000924.1;Parent=MsaT000924.1
Chr1	exon	12514871	12514971	12514871	ID=MsaT000924.1.exon4;Parent=MsaT000924.1
Chr1	CDS	12514871	12514971	12514871	ID=cds.MsaT000924.1;Parent=MsaT000924.1
Chr1	exon	12515776	12516005	12515776	ID=MsaT000924.1.exon3;Parent=MsaT000924.1
Chr1	CDS	12515776	12516005	12515776	ID=cds.MsaT000924.1;Parent=MsaT000924.1
Chr1	exon	12516545	12516625	12516545	ID=MsaT000924.1.exon2;Parent=MsaT000924.1
Chr1	CDS	12516545	12516625	12516545	ID=cds.MsaT000924.1;Parent=MsaT000924.1
Chr1	exon	12516887	12517127	12516887	ID=MsaT000924.1.exon1;Parent=MsaT000924.1
Chr1	CDS	12516887	12517127	12516887	ID=cds.MsaT000924.1;Parent=MsaT000924.1

Gene Sequence

>MsaT000924.1

ATGGCTTCACCACTTCGCCGAAGAAGAATTCCAGCTCCCAAAAAACAATCCACAACCTTCAACGATGATGACGACGTCGTTTGCCAGAAATGCAACTCCGGCAAATCCCCAACTAAGTTGCTTCTTTGTGACAATTGCGACAACGGCTACCATCTCTTCTGCCTCACACCTATCCTCCCTTCTGTACCTAAATCCTCTTGGTTTTGCCCTTCCTGCTCTCACAACCCCAAAATCCCCAAATCTTTTCCTCTTGTACAAACCAAAATTATTGATTTCTTCAAGATTCAAAGATCCGTTGATGCATCTAAGATTCTGAATCATGATTCAAAGAAGAAGAGAAAGCGGAGTAGTAGTTTGGTGGTTTCAAAAAAGAAAAGGAAGTTGTTGGTATTTGTGCCAAATGATGATTTGAAGAGGAGATTAGAACAGATGGCGTCGTTGGCAACCGCGTTGACTGCTACTAAAACGGAGTTCAGTAATGAGCTTACGTACATGCCTGGAATGGCGCCAAGGGATGCTAATTCTCCTGTTCTTGAACGTGGAGGAATGCAGGTCTTATCAAAAGAAGACACCGAGACTCTTAACTTGTGCAGAAGTATGATGGAAAGAGGAGAATGTCCGCCCCTCATGGTTGTTTATGATCCTGTAGAAGGGTTCACTGTTGAAGCAGACAAGTCCATAAAAGATTTGACAATAATAACAGAATATGTTGGAGATGTAGACTTTTTGAAGAATCGGGAATATGATGATGGAGATAGCATTATGACACTTCTTTCTGCTTCTAATCCTTCACAGTCACTGGTCATCTGCCCAGATAAACGTAGCAATATAGCACGTTTCATTAATGGAATCAACAATCACACACCTGAAGGAAAAAAGAAGCAGAACCTGAAATGCGTGAGATATAATGTTGATGGTGAATGTCGAGTTCTTTTAATTGCAAATAGAGATATAGCAAAGGGAGAAAGAGTTGAAGTTGCTCCAATGCAATGTGTGATTGCATCCATATCTTGTACTAGGGTGTTCAACCGCCTTCTAAGTGTTAGACTCTGCCCTGGTTGGTGTTCCTCCATATCTGCGGTCTTATCGAAAGAAGACACCGAGACTCTAAACATGTGCAGAAGTATGATGGAAAGAGGAGAATGTCCGCCCCTCATGGTTGTTTTTGATCCTGTAGAAGGGTTCACTGTAGAAGCAAATAAATCCATAAAAGAGTCGACCATAACAACAGAATATGTTGTAGATGTAGACTTTTTAAAGAATCCGTAA

Protein sequence

>MsaT000924.1

MASPLRRRRIPAPKKQSTTFNDDDDVVCQKCNSGKSPTKLLLCDNCDNGYHLFCLTPILPSVPKSSWFCPSCSHNPKIPKSFPLVQTKIIDFFKIQRSVDASKILNHDSKKKRKRSSSLVVSKKKRKLLVFVPNDDLKRRLEQMASLATALTATKTEFSNELTYMPGMAPRDANSPVLERGGMQVLSKEDTETLNLCRSMMERGECPPLMVVYDPVEGFTVEADKSIKDLTIITEYVGDVDFLKNREYDDGDSIMTLLSASNPSQSLVICPDKRSNIARFINGINNHTPEGKKKQNLKCVRYNVDGECRVLLIANRDIAKGERVEVAPMQCVIASISCTRVFNRLLSVRLCPGWCSSISAVLSKEDTETLNMCRSMMERGECPPLMVVFDPVEGFTVEANKSIKESTITTEYVVDVDFLKNP*