AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG000955

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG000955 MtrunA17_Chr1g0146301 96.199 684 7 4 1 665 1 684 0.0 1361
MsaG000955 MtrunA17_Chr3g0140431 85.643 599 70 2 81 665 2 598 0.0 1086
MsaG000955 MtrunA17_Chr7g0214531 80.314 574 103 2 97 664 29 598 0.0 996
MsaG000955 MtrunA17_Chr1g0213321 78.735 569 116 2 97 664 111 675 0.0 974
MsaG000955 MtrunA17_Chr8g0340491 77.778 576 117 3 97 665 120 691 0.0 938
MsaG000955 MtrunA17_Chr7g0253211 75.567 573 131 2 97 664 316 884 0.0 933
MsaG000955 MtrunA17_Chr4g0071031 38.828 273 156 5 105 374 9 273 3.92e-59 205
MsaG000955 MtrunA17_Chr2g0329981 38.828 273 156 5 105 374 9 273 8.59e-59 205
MsaG000955 MtrunA17_Chr2g0299661 38.462 273 157 5 105 374 9 273 1.04e-57 202
MsaG000955 MtrunA17_Chr8g0342821 37.828 267 154 6 105 368 9 266 3.32e-56 198
MsaG000955 MtrunA17_Chr7g0261501 38.095 273 158 5 105 374 9 273 4.02e-56 197
MsaG000955 MtrunA17_Chr1g0166821 37.634 279 161 6 99 374 6 274 6.89e-56 197
MsaG000955 MtrunA17_Chr1g0187571 38.710 279 158 6 99 374 6 274 7.42e-56 197
MsaG000955 MtrunA17_Chr1g0183631 37.634 279 161 6 99 374 5 273 2.39e-54 192
MsaG000955 MtrunA17_Chr6g0486611 37.363 273 160 5 105 374 9 273 6.42e-54 191
MsaG000955 MtrunA17_Chr1g0146151 36.918 279 163 6 99 374 5 273 1.29e-53 189
MsaG000955 MtrunA17_Chr8g0390211 37.276 279 162 6 99 374 5 273 2.61e-53 189
MsaG000955 MtrunA17_Chr1g0167261 37.102 283 163 6 97 374 4 276 1.21e-52 185
MsaG000955 MtrunA17_Chr3g0140071 36.201 279 165 6 99 374 5 273 1.41e-52 187
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG000955 AT2G25760.1 76.065 681 136 9 3 665 2 673 0.0 1071
MsaG000955 AT2G25760.2 75.439 684 138 10 3 665 2 676 0.0 1061
MsaG000955 AT3G13670.1 68.467 685 181 4 16 665 19 703 0.0 982
MsaG000955 AT3G03940.1 75.916 573 129 2 97 664 132 700 0.0 946
MsaG000955 AT5G18190.2 74.520 573 137 2 97 664 122 690 0.0 931
MsaG000955 AT5G18190.1 74.520 573 137 2 97 664 122 690 0.0 931
MsaG000955 AT3G03930.1 71.250 240 69 0 425 664 48 287 2.13e-123 368
MsaG000955 AT2G19470.1 38.828 273 156 5 105 374 9 273 3.35e-58 202
MsaG000955 AT5G57015.1 38.095 273 158 5 105 374 9 273 7.35e-58 202
MsaG000955 AT4G26100.1 38.095 273 158 5 105 374 9 273 7.72e-58 202
MsaG000955 AT3G23340.1 38.710 279 158 6 99 374 5 273 8.01e-58 202
MsaG000955 AT1G03930.1 37.993 279 160 6 99 374 5 273 6.80e-57 200
MsaG000955 AT4G14340.2 38.710 279 158 6 99 374 11 279 7.04e-57 197
MsaG000955 AT4G14340.1 38.710 279 158 6 99 374 11 279 1.11e-56 199
MsaG000955 AT1G72710.1 38.462 273 156 6 105 374 9 272 5.81e-56 197
MsaG000955 AT5G44100.1 36.918 279 163 6 99 374 5 273 1.06e-55 197
MsaG000955 AT5G44100.2 36.918 279 163 6 99 374 5 273 1.06e-55 197
MsaG000955 AT4G28540.1 37.276 279 162 6 99 374 9 277 1.56e-55 197
MsaG000955 AT5G43320.1 37.276 279 162 6 99 374 5 273 5.82e-54 192
MsaG000955 AT4G28880.3 37.276 279 162 6 99 374 5 273 9.14e-54 190
MsaG000955 AT4G28880.2 37.276 279 162 6 99 374 5 273 9.14e-54 190
MsaG000955 AT4G28880.1 37.276 279 162 6 99 374 5 273 9.14e-54 190
MsaG000955 AT4G28880.4 37.276 279 162 6 99 374 5 273 9.14e-54 190
MsaG000955 AT4G28860.2 37.276 279 162 6 99 374 5 273 1.42e-53 189
MsaG000955 AT5G43320.2 37.276 279 162 6 99 374 5 273 2.29e-53 187
MsaG000955 AT5G43320.3 37.276 279 162 6 99 374 5 273 2.29e-53 187
MsaG000955 AT4G28860.1 37.276 279 162 6 99 374 5 273 2.44e-53 189
MsaG000955 AT1G04440.1 36.559 279 164 6 99 374 5 273 7.54e-53 189
MsaG000955 AT3G23340.2 38.314 261 150 5 117 374 7 259 3.16e-51 184
MsaG000955 AT4G08800.1 33.333 273 139 6 105 374 9 241 6.65e-42 154

Find 174 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATATATATTTGGCATAATT+TGG 0.158062 1:+12905992 None:intergenic
CTGAGCACCGTCAGTGTTTA+TGG 0.167427 1:+12905928 None:intergenic
CCCATTCTGACTTTCTTCTT+TGG 0.171287 1:+12905751 None:intergenic
CTCACGTGCCGGAACAAATT+AGG 0.172612 1:-12908792 MsaT000955.1:CDS
ATATTCTTGGCCCTAGTTTA+TGG 0.174994 1:-12907331 MsaT000955.1:CDS
TAACCTTTGGGCACTGATTA+TGG 0.191586 1:-12905498 MsaT000955.1:CDS
ATCGAAAAGTGATATATATT+TGG 0.208565 1:+12905982 None:intergenic
AGAATGCATCTTCTCTAATA+TGG 0.254263 1:+12907128 None:intergenic
TGCTGTTAAGCTTATTTATC+AGG 0.256618 1:-12905824 MsaT000955.1:intron
CTACCTCGTGTAGCGGGTTC+GGG 0.258671 1:+12908907 None:intergenic
ACCAGTAAAGGGTGTAATTA+TGG 0.259169 1:-12907630 MsaT000955.1:CDS
TTCTGATAAGCAAGAGATTA+TGG 0.259548 1:-12908767 MsaT000955.1:CDS
TAGGTTGTTGTTCTAACCTT+TGG 0.265781 1:-12905511 MsaT000955.1:CDS
TCCATAATTACACCCTTTAC+TGG 0.271957 1:+12907629 None:intergenic
TCGGGTTTAGCGATCGGGTT+CGG 0.281544 1:+12908925 None:intergenic
TCTTGTTTGCAAGAAGAAAA+TGG 0.283561 1:-12906104 MsaT000955.1:CDS
GCCCTAGTTTATGGGATGTT+TGG 0.283968 1:-12907322 MsaT000955.1:CDS
GAAGAAAACCTTTGCTTCAT+AGG 0.289095 1:+12905685 None:intergenic
ATGGGTCTAATGTCTGGATT+TGG 0.294234 1:+12905947 None:intergenic
TCTACCTCGTGTAGCGGGTT+CGG 0.313908 1:+12908906 None:intergenic
TATTCTTGGCCCTAGTTTAT+GGG 0.314228 1:-12907330 MsaT000955.1:CDS
CGGGTTTAGCGATCGGGTTC+GGG 0.318336 1:+12908926 None:intergenic
TCACGTGCCGGAACAAATTA+GGG 0.325179 1:-12908791 MsaT000955.1:CDS
TGAGCACCGTCAGTGTTTAT+GGG 0.331178 1:+12905929 None:intergenic
TTGTTTGTATGTAGTGTATT+AGG 0.339100 1:-12907513 MsaT000955.1:intron
AAGCTAGGGAAAGGAGGTTT+TGG 0.342457 1:-12908494 MsaT000955.1:CDS
TTTCTGGTCGATCTTGGTTT+AGG 0.344239 1:-12906623 MsaT000955.1:intron
ATCAACGTCCAGATGTATTT+AGG 0.344411 1:-12906446 MsaT000955.1:intron
TATAAGATTGCTGCAAGTAT+TGG 0.346054 1:+12905023 None:intergenic
ACACTAATCCATTGTGTTGC+TGG 0.346898 1:+12905724 None:intergenic
TTGCAGGTCATGGATATTCT+TGG 0.355219 1:-12907344 MsaT000955.1:intron
AGGTTGTTGTTCTAACCTTT+GGG 0.362175 1:-12905510 MsaT000955.1:CDS
TGGCCACTGCTGGAAGTAGA+TGG 0.368491 1:-12904931 MsaT000955.1:CDS
CTTTAGGATGTCTGCTGAAA+TGG 0.375494 1:-12907178 MsaT000955.1:intron
ATAAGATTGCTGCAAGTATT+GGG 0.378251 1:+12905024 None:intergenic
TTCTTCGCGGACGGCTACCC+TGG 0.381224 1:-12906235 MsaT000955.1:CDS
TCAGAAACCCTAATTTGTTC+CGG 0.382437 1:+12908784 None:intergenic
GTGTTTATGGGTCTAATGTC+TGG 0.388332 1:+12905941 None:intergenic
ATAAATTACCTAAATACATC+TGG 0.391749 1:+12906438 None:intergenic
AGGTAGCAGAAGAGATGATT+TGG 0.392165 1:-12906285 MsaT000955.1:CDS
ATACTTGCAGCAATCTTATA+AGG 0.401113 1:-12905020 MsaT000955.1:CDS
TAGCTGCAGGGTGAAAATAA+AGG 0.408921 1:-12906130 MsaT000955.1:intron
GGGAGAACCGCAGCTGATAA+AGG 0.410075 1:-12908721 MsaT000955.1:CDS
CGGGTTCGGGTTTAGCGATC+GGG 0.416775 1:+12908920 None:intergenic
GTTTATGTTGGTCGTCGTAT+TGG 0.427782 1:-12908467 MsaT000955.1:CDS
ATTGTTATGTCCCGTGGTGC+TGG 0.431009 1:-12904905 MsaT000955.1:CDS
CTGATTATGGATGCCGGCAC+TGG 0.434575 1:-12905485 MsaT000955.1:CDS
ACGTGCAGCGGCGGCGGCGA+TGG 0.440494 1:+12908852 None:intergenic
TATATTGCATCTATTTGTTA+CGG 0.443891 1:-12904486 MsaT000955.1:CDS
TATGACCTGGTATCCTTGCC+AGG 0.447271 1:+12906217 None:intergenic
GTTAAGCTTATTTATCAGGT+TGG 0.450203 1:-12905820 MsaT000955.1:intron
ACAAGGGACGGCAAGGTGAT+TGG 0.451632 1:-12907461 MsaT000955.1:CDS
CGGCGGCGATGGTGTTGTTA+CGG 0.451967 1:+12908863 None:intergenic
GCGGGTTCGGGTTTAGCGAT+CGG 0.452422 1:+12908919 None:intergenic
TTGATCACGTGCAGCGGCGG+CGG 0.456771 1:+12908846 None:intergenic
GCCATAGCAGGATCTAATAG+TGG 0.459499 1:-12905234 MsaT000955.1:CDS
TTTGAGCATAAAACCAGTAA+AGG 0.460416 1:-12907642 MsaT000955.1:CDS
TTTACCTTGACATGAGTACT+AGG 0.461394 1:+12904603 None:intergenic
GGGTTATTTATCAGTAGTGT+AGG 0.469943 1:-12905530 MsaT000955.1:CDS
AACTCAATAAACTGCCGAAA+AGG 0.471641 1:+12906043 None:intergenic
ATCGAAAAGGGATATGAAGA+TGG 0.473461 1:-12905551 MsaT000955.1:CDS
TCGGCAGTTTATTGAGTTCG+TGG 0.473854 1:-12906038 MsaT000955.1:CDS
TACAGAGTAGATAGAAAGCT+AGG 0.474012 1:-12908509 MsaT000955.1:CDS
CTCATCCTTCTTCCTTCACA+AGG 0.477955 1:-12905438 MsaT000955.1:intron
CTCATCATCATCTCCTTCCA+TGG 0.479092 1:+12905777 None:intergenic
GTCACTGCTATGGCCACTGC+TGG 0.481033 1:-12904941 MsaT000955.1:CDS
GCCAAAGAAGAAAGTCAGAA+TGG 0.481635 1:-12905752 MsaT000955.1:CDS
AACAAATAGATGCAATATAG+AGG 0.484055 1:+12904490 None:intergenic
TTGAGCATAAAACCAGTAAA+GGG 0.485067 1:-12907641 MsaT000955.1:CDS
TGGACCTCCTTACGAGTGGC+AGG 0.485361 1:-12907610 MsaT000955.1:CDS
CGTCGTATTGGTGCCGGTGC+CGG 0.485454 1:-12908455 MsaT000955.1:CDS
GTGAATCATTTCCATTCAAG+TGG 0.485946 1:-12904994 MsaT000955.1:CDS
GAATACCTTCACTAGGATAC+AGG 0.489180 1:+12904757 None:intergenic
AGGGTAGCCGTCCGCGAAGA+AGG 0.489461 1:+12906237 None:intergenic
AACAACCTTGTGAAGGAAGA+AGG 0.492459 1:+12905433 None:intergenic
GTTGTCCCAACACATCGAAA+AGG 0.495024 1:-12905564 MsaT000955.1:CDS
GTGCAACAGGTTCAGGTGGG+TGG 0.497947 1:-12908542 MsaT000955.1:intron
GATGACTGTGATAGCGGCGG+TGG 0.500267 1:-12908742 MsaT000955.1:CDS
AGGAATGGATTATGGAACAG+TGG 0.508088 1:-12905278 MsaT000955.1:CDS
GCTAGTGTACATGCTCATCT+TGG 0.512428 1:-12906314 MsaT000955.1:CDS
TATTACATTAGTGCCATAGC+AGG 0.514536 1:-12905246 MsaT000955.1:CDS
GATAGAAAGCTAGGGAAAGG+AGG 0.516277 1:-12908500 MsaT000955.1:CDS
ATCTGAAAATCCAGCACCAC+GGG 0.518126 1:+12904895 None:intergenic
TTCCAAACATCCCATAAACT+AGG 0.518224 1:+12907320 None:intergenic
GTTTCTTGAGTTTCATCAGT+CGG 0.518437 1:+12904645 None:intergenic
CCTCTTCTACCTCGTGTAGC+GGG 0.519563 1:+12908901 None:intergenic
CATGCTCATCTTGGTAGGAC+AGG 0.525496 1:-12906305 MsaT000955.1:CDS
CCAAAGAAGAAAGTCAGAAT+GGG 0.530872 1:-12905751 MsaT000955.1:CDS
AACCGCAGCTGATAAAGGTC+CGG 0.531660 1:-12908716 MsaT000955.1:intron
ATAAACTGCCGAAAAGGTGC+GGG 0.538646 1:+12906049 None:intergenic
TTTGCCATGCAGCAACTCGT+TGG 0.539312 1:-12906503 MsaT000955.1:intron
TTGTCCCAACACATCGAAAA+GGG 0.539526 1:-12905563 MsaT000955.1:CDS
AAAACCTTTGCTTCATAGGT+CGG 0.541274 1:+12905689 None:intergenic
CGCGGACGGCTACCCTGGCA+AGG 0.543822 1:-12906230 MsaT000955.1:CDS
AAACACTGACGGTGCTCAGA+AGG 0.548675 1:-12905924 MsaT000955.1:intron
CGTCCCTTGTAATGAACTCG+TGG 0.549783 1:+12907474 None:intergenic
GCTACCCTGGCAAGGATACC+AGG 0.551712 1:-12906222 MsaT000955.1:intron
TACTAAGAACAAGAGCGGCC+TGG 0.553288 1:+12904682 None:intergenic
GTGAAGGTATTCATCGAAGG+TGG 0.556433 1:-12904746 MsaT000955.1:CDS
AAACGAACAACCTCACGTGC+CGG 0.557745 1:-12908803 MsaT000955.1:CDS
GCATATCAGCAACATTGTAA+TGG 0.559923 1:+12905588 None:intergenic
CCCGCTACACGAGGTAGAAG+AGG 0.561644 1:-12908901 MsaT000955.1:CDS
TTTGTATGTAGTGTATTAGG+AGG 0.562063 1:-12907510 MsaT000955.1:intron
TCCAAACATCCCATAAACTA+GGG 0.562426 1:+12907321 None:intergenic
ACCTCTTCTACCTCGTGTAG+CGG 0.562642 1:+12908900 None:intergenic
GTAGATAGAAAGCTAGGGAA+AGG 0.563147 1:-12908503 MsaT000955.1:CDS
GGAATGGATTATGGAACAGT+GGG 0.563150 1:-12905277 MsaT000955.1:CDS
TGCCGGTGCCGGTGCTGTTG+AGG 0.563693 1:-12908444 MsaT000955.1:intron
TTGTAAACCTGCCACTCGTA+AGG 0.564528 1:+12907603 None:intergenic
CACGAGTTCATTACAAGGGA+CGG 0.566096 1:-12907473 MsaT000955.1:CDS
GCCGAAAAGGTGCGGGACAG+AGG 0.569844 1:+12906056 None:intergenic
TGGGCACTGATTATGGATGC+CGG 0.570860 1:-12905491 MsaT000955.1:CDS
ATCTAATAGTGGGAGCTCGT+TGG 0.570915 1:-12905223 MsaT000955.1:CDS
GATTTCCTGTATCCTAGTGA+AGG 0.571117 1:-12904762 MsaT000955.1:CDS
GGCCACTGCTGGAAGTAGAT+GGG 0.571298 1:-12904930 MsaT000955.1:CDS
GTTGGTCGTCGTATTGGTGC+CGG 0.571967 1:-12908461 MsaT000955.1:CDS
CTTCGATGAATACCTTCACT+AGG 0.572280 1:+12904750 None:intergenic
AGAGGACGGTTGACCATGGA+AGG 0.574941 1:-12905790 MsaT000955.1:CDS
TACCTCAACAGCACCGGCAC+CGG 0.575462 1:+12908442 None:intergenic
CTAATTTGTTCCGGCACGTG+AGG 0.575786 1:+12908793 None:intergenic
TCGAAAAGGGATATGAAGAT+GGG 0.578550 1:-12905550 MsaT000955.1:CDS
CTATCATACCTCAACAGCAC+CGG 0.581895 1:+12908436 None:intergenic
CGTTGGCGTGATAATTCGAC+TGG 0.587524 1:-12906486 MsaT000955.1:CDS
GCAAGGTGATTGGTATGTCA+TGG 0.589006 1:-12907451 MsaT000955.1:intron
CGCGCCGACCTATGAAGCAA+AGG 0.589757 1:-12905693 MsaT000955.1:intron
AACTGCTGCTACATATGACC+AGG 0.592537 1:-12904700 MsaT000955.1:CDS
ACAGAGTAGATAGAAAGCTA+GGG 0.592579 1:-12908508 MsaT000955.1:CDS
AACCGGACCTTTATCAGCTG+CGG 0.595722 1:+12908714 None:intergenic
AATAAACTGCCGAAAAGGTG+CGG 0.597388 1:+12906048 None:intergenic
GTGTACATGCTCATCTTGGT+AGG 0.600986 1:-12906310 MsaT000955.1:CDS
TACTTGATCACGTGCAGCGG+CGG 0.603629 1:+12908843 None:intergenic
CTTTCCTAGTACTCATGTCA+AGG 0.608784 1:-12904607 MsaT000955.1:intron
TTGTGCAGTGAAACCAGTGC+CGG 0.610323 1:+12905472 None:intergenic
TGCCCATCTACTTCCAGCAG+TGG 0.611695 1:+12904928 None:intergenic
CCATAGCAGGATCTAATAGT+GGG 0.612424 1:-12905233 MsaT000955.1:CDS
CCCACTATTAGATCCTGCTA+TGG 0.617210 1:+12905233 None:intergenic
TTTCTGGTTCGAAGTTGAGG+AGG 0.618261 1:+12908979 None:intergenic
TCTCTTCATGAGTTCCCGGA+GGG 0.618779 1:+12906654 None:intergenic
GTTCATTACAAGGGACGGCA+AGG 0.622003 1:-12907468 MsaT000955.1:CDS
TATCAGGTTGGACAGAAGAG+AGG 0.622077 1:-12905808 MsaT000955.1:CDS
GAAGAGAGGACGGTTGACCA+TGG 0.624209 1:-12905794 MsaT000955.1:CDS
TTGTACTTGATCACGTGCAG+CGG 0.624708 1:+12908840 None:intergenic
TGAAGGTATTCATCGAAGGT+GGG 0.628022 1:-12904745 MsaT000955.1:CDS
TCGTTGGTTGTAATGTCGAA+AGG 0.629923 1:-12905207 MsaT000955.1:intron
TTCTCTTCATGAGTTCCCGG+AGG 0.630312 1:+12906653 None:intergenic
TGGGAATGCCAGCAACACAA+TGG 0.633757 1:-12905732 MsaT000955.1:CDS
CTAGTGAAGGTATTCATCGA+AGG 0.635960 1:-12904749 MsaT000955.1:CDS
TAGAGAAGATGCATTCTAGA+GGG 0.637214 1:-12907122 MsaT000955.1:intron
TTTGTGCAACAGGTTCAGGT+GGG 0.638924 1:-12908545 MsaT000955.1:intron
ATTTGTTTCAGATATGTGCA+TGG 0.641831 1:-12906707 MsaT000955.1:intron
ATGACCTGGTATCCTTGCCA+GGG 0.644585 1:+12906218 None:intergenic
TTAGAGAAGATGCATTCTAG+AGG 0.645638 1:-12907123 MsaT000955.1:CDS
ATTCATCGAAGGTGGGACAA+TGG 0.646078 1:-12904738 MsaT000955.1:CDS
TGGGCAATTGTTATGTCCCG+TGG 0.648130 1:-12904911 MsaT000955.1:CDS
TTTCTATCTACTCTGTACAT+CGG 0.650362 1:+12908515 None:intergenic
TGTCCCACGAGTTCATTACA+AGG 0.652591 1:-12907478 MsaT000955.1:CDS
AATGAACTCGTGGGACACCA+TGG 0.653940 1:+12907484 None:intergenic
TAAACCTGCCACTCGTAAGG+AGG 0.654486 1:+12907606 None:intergenic
CATCCATAATCAGTGCCCAA+AGG 0.657903 1:+12905495 None:intergenic
ATGACTGTGATAGCGGCGGT+GGG 0.665147 1:-12908741 MsaT000955.1:CDS
GGTGTCGTGTATAGCAATCG+AGG 0.667047 1:-12907157 MsaT000955.1:CDS
AGTGTATTAGGAGGTAGCCA+TGG 0.671950 1:-12907501 MsaT000955.1:CDS
GATCTGAAAATCCAGCACCA+CGG 0.671996 1:+12904894 None:intergenic
GTCCCTTGTAATGAACTCGT+GGG 0.673497 1:+12907475 None:intergenic
ATTACAATGTTGCTGATATG+CGG 0.677018 1:-12905586 MsaT000955.1:CDS
ATGGATGATGACTGTGATAG+CGG 0.678914 1:-12908748 MsaT000955.1:CDS
AGGTTGGACAGAAGAGAGGA+CGG 0.682501 1:-12905804 MsaT000955.1:CDS
ATTAGACCCATAAACACTGA+CGG 0.692330 1:-12905935 MsaT000955.1:CDS
GTCCCACGAGTTCATTACAA+GGG 0.693718 1:-12907477 MsaT000955.1:CDS
TGGAATACTAAGAACAAGAG+CGG 0.693783 1:+12904677 None:intergenic
TAGCAGCAGTTGAAGTAATG+CGG 0.693804 1:+12904712 None:intergenic
ATTATGGACCTCCTTACGAG+TGG 0.700752 1:-12907614 MsaT000955.1:CDS
AAACCCGAACCCGCTACACG+AGG 0.720713 1:-12908910 MsaT000955.1:CDS
GATGATGACTGTGATAGCGG+CGG 0.731600 1:-12908745 MsaT000955.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr1 gene 12904470 12909004 12904470 ID=MsaG000955
Chr1 mRNA 12904470 12909004 12904470 ID=MsaT000955.1;Parent=MsaG000955
Chr1 exon 12904470 12904529 12904470 ID=MsaT000955.1.exon16;Parent=MsaT000955.1
Chr1 CDS 12904470 12904529 12904470 ID=cds.MsaT000955.1;Parent=MsaT000955.1
Chr1 exon 12904608 12904796 12904608 ID=MsaT000955.1.exon15;Parent=MsaT000955.1
Chr1 CDS 12904608 12904796 12904608 ID=cds.MsaT000955.1;Parent=MsaT000955.1
Chr1 exon 12904892 12905049 12904892 ID=MsaT000955.1.exon14;Parent=MsaT000955.1
Chr1 CDS 12904892 12905049 12904892 ID=cds.MsaT000955.1;Parent=MsaT000955.1
Chr1 exon 12905208 12905298 12905208 ID=MsaT000955.1.exon13;Parent=MsaT000955.1
Chr1 CDS 12905208 12905298 12905208 ID=cds.MsaT000955.1;Parent=MsaT000955.1
Chr1 exon 12905439 12905613 12905439 ID=MsaT000955.1.exon12;Parent=MsaT000955.1
Chr1 CDS 12905439 12905613 12905439 ID=cds.MsaT000955.1;Parent=MsaT000955.1
Chr1 exon 12905694 12905836 12905694 ID=MsaT000955.1.exon11;Parent=MsaT000955.1
Chr1 CDS 12905694 12905836 12905694 ID=cds.MsaT000955.1;Parent=MsaT000955.1
Chr1 exon 12905925 12906143 12905925 ID=MsaT000955.1.exon10;Parent=MsaT000955.1
Chr1 CDS 12905925 12906143 12905925 ID=cds.MsaT000955.1;Parent=MsaT000955.1
Chr1 exon 12906223 12906352 12906223 ID=MsaT000955.1.exon9;Parent=MsaT000955.1
Chr1 CDS 12906223 12906352 12906223 ID=cds.MsaT000955.1;Parent=MsaT000955.1
Chr1 exon 12906447 12906513 12906447 ID=MsaT000955.1.exon8;Parent=MsaT000955.1
Chr1 CDS 12906447 12906513 12906447 ID=cds.MsaT000955.1;Parent=MsaT000955.1
Chr1 exon 12906624 12906718 12906624 ID=MsaT000955.1.exon7;Parent=MsaT000955.1
Chr1 CDS 12906624 12906718 12906624 ID=cds.MsaT000955.1;Parent=MsaT000955.1
Chr1 exon 12907123 12907194 12907123 ID=MsaT000955.1.exon6;Parent=MsaT000955.1
Chr1 CDS 12907123 12907194 12907123 ID=cds.MsaT000955.1;Parent=MsaT000955.1
Chr1 exon 12907305 12907360 12907305 ID=MsaT000955.1.exon5;Parent=MsaT000955.1
Chr1 CDS 12907305 12907360 12907305 ID=cds.MsaT000955.1;Parent=MsaT000955.1
Chr1 exon 12907452 12907521 12907452 ID=MsaT000955.1.exon4;Parent=MsaT000955.1
Chr1 CDS 12907452 12907521 12907452 ID=cds.MsaT000955.1;Parent=MsaT000955.1
Chr1 exon 12907603 12907676 12907603 ID=MsaT000955.1.exon3;Parent=MsaT000955.1
Chr1 CDS 12907603 12907676 12907603 ID=cds.MsaT000955.1;Parent=MsaT000955.1
Chr1 exon 12908445 12908555 12908445 ID=MsaT000955.1.exon2;Parent=MsaT000955.1
Chr1 CDS 12908445 12908555 12908445 ID=cds.MsaT000955.1;Parent=MsaT000955.1
Chr1 exon 12908717 12909004 12908717 ID=MsaT000955.1.exon1;Parent=MsaT000955.1
Chr1 CDS 12908717 12909004 12908717 ID=cds.MsaT000955.1;Parent=MsaT000955.1
Gene Sequence

>MsaT000955.1

ATGCCTCCTCAACTTCGAACCAGAAAAACTAAAAATAACCCGAATCCGAATCAAAACCCGAACCCGATCGCTAAACCCGAACCCGCTACACGAGGTAGAAGAGGTAGACCTGCTAAAAACCGTAACAACACCATCGCCGCCGCCGCTGCACGTGATCAAGTACAACAACAACAACAACAAAACGAACAACCTCACGTGCCGGAACAAATTAGGGTTTCTGATAAGCAAGAGATTATGGATGATGACTGTGATAGCGGCGGTGGGAGAACCGCAGCTGATAAAGGTCCGGTTCAGGTGGGTGGTTCACCGATGTACAGAGTAGATAGAAAGCTAGGGAAAGGAGGTTTTGGACAAGTTTATGTTGGTCGTCGTATTGGTGCCGGTGCCGGTGCTGTTGAGGTGGCTTTGAAATTTGAGCATAAAACCAGTAAAGGGTGTAATTATGGACCTCCTTACGAGTGGCAGGTTTACAATGTATTAGGAGGTAGCCATGGTGTCCCACGAGTTCATTACAAGGGACGGCAAGGTGATTGGTATGTCATGGTCATGGATATTCTTGGCCCTAGTTTATGGGATGTTTGGAATAACAAGACACACACGATGTCTGCTGAAATGGTGTCGTGTATAGCAATCGAGGCTGTTTCCATATTAGAGAAGATGCATTCTAGAGGATATGTGCATGGTGACGTAAAACCTGAGAACTTTTTGCTTGGCCCTCCGGGAACTCATGAAGAGAAAAAGCTTTTTCTGGTCGATCTTGGTTTAGCAACTCGTTGGCGTGATAATTCGACTGGTCTTCATGTTGATTACGATCAACGTCCAGATGTATTTAGGGGAACTGTTCGTTATGCTAGTGTACATGCTCATCTTGGTAGGACAGGTAGCAGAAGAGATGATTTGGAATCTCTTGCATACACACTAATTTTCCTTCTTCGCGGACGGCTACCCTGGCAAGGATACCAGGGTGAAAATAAAGGATTTCTTGTTTGCAAGAAGAAAATGGCCACCTCGCCAGAAAATATGTGTATCCTCTGTCCCGCACCTTTTCGGCAGTTTATTGAGTTCGTGGTGAATTTGAAGTTCGACGAAGAACCAAATTATGCCAAATATATATCACTTTTCGATGGAATTGTTGGTCCAAATCCAGACATTAGACCCATAAACACTGACGGTGCTCAGAAGCTTATTTATCAGGTTGGACAGAAGAGAGGACGGTTGACCATGGAAGGAGATGATGATGAGCAGCCAAAGAAGAAAGTCAGAATGGGAATGCCAGCAACACAATGGATTAGTGTGTACAATGCGCGCCGACCTATGAAGCAAAGGTACCATTACAATGTTGCTGATATGCGGTTGTCCCAACACATCGAAAAGGGATATGAAGATGGGTTATTTATCAGTAGTGTAGGTTGTTGTTCTAACCTTTGGGCACTGATTATGGATGCCGGCACTGGTTTCACTGCACAAGTTTATGAACTCTCATCCTTCTTCCTTCACAAGGAATGGATTATGGAACAGTGGGAAAAAAATTATTACATTAGTGCCATAGCAGGATCTAATAGTGGGAGCTCGTTGGTTGTAATGTCGAAAGGGACCCAATACTTGCAGCAATCTTATAAGGTCAGTGAATCATTTCCATTCAAGTGGATTAATAAAAAATGGAAAGAAGGATTTTTCGTCACTGCTATGGCCACTGCTGGAAGTAGATGGGCAATTGTTATGTCCCGTGGTGCTGGATTTTCAGATCAGGTTGTGGAGCTTGATTTCCTGTATCCTAGTGAAGGTATTCATCGAAGGTGGGACAATGGTTACCGCATTACTTCAACTGCTGCTACATATGACCAGGCCGCTCTTGTTCTTAGTATTCCAAGAAGAAAACCGACTGATGAAACTCAAGAAACACTTCGCACATCTGACTTTCCTAGTACTCATGTCAAGGAAAAATGGGCAAAGAACCTCTATATTGCATCTATTTGTTACGGAAGAACTGTTTCATGA
Protein sequence

>MsaT000955.1

MPPQLRTRKTKNNPNPNQNPNPIAKPEPATRGRRGRPAKNRNNTIAAAAARDQVQQQQQQNEQPHVPEQIRVSDKQEIMDDDCDSGGGRTAADKGPVQVGGSPMYRVDRKLGKGGFGQVYVGRRIGAGAGAVEVALKFEHKTSKGCNYGPPYEWQVYNVLGGSHGVPRVHYKGRQGDWYVMVMDILGPSLWDVWNNKTHTMSAEMVSCIAIEAVSILEKMHSRGYVHGDVKPENFLLGPPGTHEEKKLFLVDLGLATRWRDNSTGLHVDYDQRPDVFRGTVRYASVHAHLGRTGSRRDDLESLAYTLIFLLRGRLPWQGYQGENKGFLVCKKKMATSPENMCILCPAPFRQFIEFVVNLKFDEEPNYAKYISLFDGIVGPNPDIRPINTDGAQKLIYQVGQKRGRLTMEGDDDEQPKKKVRMGMPATQWISVYNARRPMKQRYHYNVADMRLSQHIEKGYEDGLFISSVGCCSNLWALIMDAGTGFTAQVYELSSFFLHKEWIMEQWEKNYYISAIAGSNSGSSLVVMSKGTQYLQQSYKVSESFPFKWINKKWKEGFFVTAMATAGSRWAIVMSRGAGFSDQVVELDFLYPSEGIHRRWDNGYRITSTAATYDQAALVLSIPRRKPTDETQETLRTSDFPSTHVKEKWAKNLYIASICYGRTVS*