AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG000960


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG000960 MtrunA17_Chr1g0146231 89.398 349 26 4 1 343 1 344 0.0 601
MsaG000960 MtrunA17_Chr3g0140331 51.488 336 113 9 19 343 68 364 5.05e-93 281
MsaG000960 MtrunA17_Chr8g0340401 43.567 342 138 13 23 343 35 342 1.47e-69 221
MsaG000960 MtrunA17_Chr8g0372291 45.729 199 90 5 105 303 108 288 1.48e-43 152
MsaG000960 MtrunA17_Chr6g0461591 38.696 230 106 7 104 299 30 258 1.01e-39 140
MsaG000960 MtrunA17_Chr4g0035891 32.390 318 143 10 5 302 15 280 5.91e-38 137
MsaG000960 MtrunA17_Chr5g0404981 43.498 223 92 7 105 303 158 370 1.55e-36 135
MsaG000960 MtrunA17_Chr3g0145121 36.765 204 111 3 101 303 99 285 2.34e-35 130
MsaG000960 MtrunA17_Chr1g0169431 78.873 71 15 0 232 302 171 241 7.32e-35 127
MsaG000960 MtrunA17_Chr1g0199671 33.438 317 146 11 27 297 9 306 7.62e-35 130
MsaG000960 MtrunA17_Chr7g0253101 39.091 220 94 8 108 297 43 252 4.40e-33 123
MsaG000960 MtrunA17_Chr5g0395391 74.648 71 18 0 232 302 269 339 5.78e-33 125
MsaG000960 MtrunA17_Chr5g0395391 72.222 72 20 0 232 303 167 238 1.22e-29 116
MsaG000960 MtrunA17_Chr1g0213161 55.963 109 43 2 212 319 130 234 3.93e-32 120
MsaG000960 MtrunA17_Chr2g0326771 76.923 65 15 0 233 297 216 280 1.29e-31 120
MsaG000960 MtrunA17_Chr4g0028961 73.134 67 18 0 233 299 232 298 6.78e-31 119
MsaG000960 MtrunA17_Chr7g0232671 62.903 62 23 0 233 294 187 248 2.97e-21 91.3
MsaG000960 MtrunA17_Chr7g0232701 58.065 62 26 0 233 294 73 134 2.72e-19 83.2
MsaG000960 MtrunA17_Chr7g0232681 56.923 65 27 1 230 294 130 193 7.74e-19 83.6
MsaG000960 MtrunA17_Chr7g0271861 70.588 34 10 0 235 268 24 57 2.31e-11 60.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG000960 AT5G25830.1 55.598 259 98 7 102 343 73 331 1.25e-83 256
MsaG000960 AT4G32890.1 42.899 338 133 10 21 343 16 308 2.88e-74 232
MsaG000960 AT2G45050.1 41.631 233 79 7 98 313 65 257 2.12e-44 153
MsaG000960 AT3G60530.1 45.411 207 67 7 98 301 63 226 1.04e-43 151
MsaG000960 AT5G66320.2 37.455 275 115 8 69 315 86 331 1.69e-37 137
MsaG000960 AT5G66320.1 37.455 275 115 8 69 315 86 331 1.69e-37 137
MsaG000960 AT4G34680.1 37.850 214 87 5 91 302 80 249 1.94e-35 130
MsaG000960 AT4G34680.2 37.850 214 87 5 91 302 80 249 1.94e-35 130
MsaG000960 AT4G36240.1 41.176 204 69 6 106 300 70 231 2.90e-34 126
MsaG000960 AT3G24050.1 74.286 70 18 0 232 301 193 262 2.59e-33 124
MsaG000960 AT1G08010.2 65.476 84 23 1 228 305 215 298 6.37e-33 124
MsaG000960 AT1G08010.1 65.476 84 23 1 228 305 215 298 6.37e-33 124
MsaG000960 AT1G08010.3 65.476 84 23 1 228 305 215 298 6.37e-33 124
MsaG000960 AT1G08000.2 70.833 72 21 0 228 299 213 284 5.26e-31 119
MsaG000960 AT1G08000.1 70.833 72 21 0 228 299 213 284 5.26e-31 119
MsaG000960 AT3G54810.1 62.963 81 30 0 233 313 229 309 6.25e-31 119
MsaG000960 AT3G54810.2 62.963 81 30 0 233 313 229 309 6.25e-31 119
MsaG000960 AT3G51080.1 32.268 313 161 10 6 301 11 289 7.84e-30 116
MsaG000960 AT3G45170.1 43.846 130 62 2 181 301 56 183 1.08e-26 105
MsaG000960 AT2G28340.1 69.841 63 19 0 235 297 193 255 1.98e-26 106

Find 117 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCGTCACTGTCCTTTCTTCT+TGG 0.090798 1:+13014398 None:intergenic
AAAACGATTCCTCATCAATA+TGG 0.237248 1:+13014711 None:intergenic
TCAAAAGATGCAACTGATAT+CGG 0.281999 1:-13014675 MsaT000960.1:CDS
GCAGTCTTTGCCTGCTTCCT+TGG 0.291400 1:+13014422 None:intergenic
TACTCTGATGTTGTAAAATC+TGG 0.292416 1:+13014120 None:intergenic
CCAATATCAGGAACGACATT+AGG 0.299571 1:+13015289 None:intergenic
CCTAATGTCGTTCCTGATAT+TGG 0.316834 1:-13015289 MsaT000960.1:CDS
TATATTCAACAAGGACGTTT+TGG 0.333107 1:-13014588 MsaT000960.1:CDS
CTAGCTGCAGGCCTGTATTC+AGG 0.333508 1:+13014242 None:intergenic
CTCGAGAGACCTGTGTGTTC+CGG 0.348887 1:-13015243 MsaT000960.1:CDS
CGACGTCATTGTTGAGGTTA+AGG 0.370723 1:+13015434 None:intergenic
TGTTGAATATAGGACTGTTT+CGG 0.382615 1:+13014600 None:intergenic
AAGACACCACAATGGCGAAC+CGG 0.386976 1:-13014329 MsaT000960.1:CDS
TTACTTGATTCACCAACATA+TGG 0.405233 1:-13014069 MsaT000960.1:CDS
ACGATGCGAGTTAGAGTGTT+TGG 0.406152 1:+13014202 None:intergenic
AGGACTGTTTCGGTTGGGTT+GGG 0.406165 1:+13014610 None:intergenic
GTTGGGTTGGGAAAGCTCAC+GGG 0.412320 1:+13014622 None:intergenic
CAATGGCGAACCGGGCCTAA+TGG 0.420213 1:-13014320 MsaT000960.1:CDS
GGAGGATGACTTAGCGGCCC+TGG 0.433321 1:-13014744 MsaT000960.1:CDS
CAATATCAGGAACGACATTA+GGG 0.434396 1:+13015290 None:intergenic
CACGGTAACGGTGGAGGAAG+AGG 0.434827 1:+13015351 None:intergenic
AAAACGTCCTTGTTGAATAT+AGG 0.436633 1:+13014590 None:intergenic
CTCTTGCTACGGGCCTTAGC+TGG 0.442400 1:+13014563 None:intergenic
TGTCGGAGGAAAAGGTGGTT+TGG 0.443866 1:+13015491 None:intergenic
CACACAGGTCTCTCGAGAAA+TGG 0.447481 1:+13015249 None:intergenic
GGTTGGGTTGGGAAAGCTCA+CGG 0.448750 1:+13014621 None:intergenic
ATGACTTAGCGGCCCTGGAC+TGG 0.449566 1:-13014739 MsaT000960.1:CDS
TACATCATCTGCCTAAAATC+AGG 0.450652 1:+13014044 None:intergenic
AATATAGGACTGTTTCGGTT+GGG 0.459476 1:+13014605 None:intergenic
TAGGACTGTTTCGGTTGGGT+TGG 0.462649 1:+13014609 None:intergenic
AGTGTTGTTGTCGGAGGAAA+AGG 0.462952 1:+13015483 None:intergenic
AAGGACAGTGACGGAGGGTC+AGG 0.466229 1:-13014389 MsaT000960.1:CDS
GAGGCAGAAGGAAATGTTGC+GGG 0.466463 1:-13014162 MsaT000960.1:CDS
TACTTGATTCACCAACATAT+GGG 0.466490 1:-13014068 MsaT000960.1:CDS
GTCGATTGGTGCCTGAATAC+AGG 0.470023 1:-13014253 MsaT000960.1:CDS
AGACACCACAATGGCGAACC+GGG 0.471491 1:-13014328 MsaT000960.1:CDS
GCCCACGAGTCCTCTTGCTA+CGG 0.473059 1:+13014552 None:intergenic
GAATATAGGACTGTTTCGGT+TGG 0.481982 1:+13014604 None:intergenic
GCTCACGGGGTTCTTCATCC+TGG 0.486213 1:+13014636 None:intergenic
GTTAGCTCGTCATCATTGTC+CGG 0.486841 1:-13015319 MsaT000960.1:CDS
CAAAAGATGCAACTGATATC+GGG 0.494290 1:-13014674 MsaT000960.1:CDS
AAACGATTCCTCATCAATAT+GGG 0.498163 1:+13014712 None:intergenic
GACGTGAGCTCCAGTCGCAA+GGG 0.498267 1:+13014528 None:intergenic
TGTTTGGTCAGCACAAATGT+AGG 0.504596 1:+13014218 None:intergenic
ATCGTAAAGTGTTGGAGCTA+CGG 0.505579 1:-13014184 MsaT000960.1:CDS
AAGGTTGGTGTCGGAAATGA+TGG 0.507635 1:+13015399 None:intergenic
GGTGGTTTGGAAAATGTTGT+TGG 0.508256 1:+13015504 None:intergenic
CATATACACATATAATATAC+CGG 0.516324 1:+13015224 None:intergenic
TACCGACAATTTCATTGTTG+AGG 0.524862 1:-13015461 MsaT000960.1:CDS
ATTGACCACGGTAACGGTGG+AGG 0.527850 1:+13015345 None:intergenic
GCCCGTAGCAAGAGGACTCG+TGG 0.533598 1:-13014554 MsaT000960.1:CDS
AAGATACAAGTCGGGTCGAT+TGG 0.534399 1:-13014267 MsaT000960.1:CDS
CCCACGAGTCCTCTTGCTAC+GGG 0.537511 1:+13014553 None:intergenic
GGGCATGAAGGTGCGAAACC+AGG 0.537637 1:-13014654 MsaT000960.1:CDS
GACGGAGGGTCAGGTGGAGA+TGG 0.539597 1:-13014380 MsaT000960.1:CDS
GTTGTTGTCGGAGGAAAAGG+TGG 0.541025 1:+13015486 None:intergenic
TGGAACAGTGACGGTAGGTA+AGG 0.543018 1:+13014442 None:intergenic
AACAGTGACGGTAGGTAAGG+TGG 0.543787 1:+13014445 None:intergenic
GAGGGTCAGGTGGAGATGGT+CGG 0.544742 1:-13014376 MsaT000960.1:CDS
CGTCACTGTTCCAAGGAAGC+AGG 0.545318 1:-13014432 MsaT000960.1:CDS
AATCAAGTAATCATCATGGT+TGG 0.550610 1:+13014082 None:intergenic
CAATATGGGAAATCCAGTCC+AGG 0.555569 1:+13014726 None:intergenic
AAAGACTGCTCCAAGAAGAA+AGG 0.555772 1:-13014408 MsaT000960.1:CDS
AACGCTATTGACCACGGTAA+CGG 0.557909 1:+13015339 None:intergenic
GACAGTGACGGAGGGTCAGG+TGG 0.558691 1:-13014386 MsaT000960.1:CDS
AGTTGATTGTGGCTGAGTCA+AGG 0.559211 1:+13015380 None:intergenic
ACAAATGTAGGGCTAGCTGC+AGG 0.560737 1:+13014230 None:intergenic
AGACGTGAGCTCCAGTCGCA+AGG 0.560751 1:+13014527 None:intergenic
ACAGTGACGGTAGGTAAGGT+GGG 0.561238 1:+13014446 None:intergenic
GAAATAGGAGGATGACTTAG+CGG 0.565643 1:-13014750 MsaT000960.1:intron
GTCCTCAACAATGAAATTGT+CGG 0.566137 1:+13015459 None:intergenic
GCAACTGATATCGGGCATGA+AGG 0.569194 1:-13014666 MsaT000960.1:CDS
TAAAATCAGGTCCCATATGT+TGG 0.573858 1:+13014057 None:intergenic
GTTTGGTCAGCACAAATGTA+GGG 0.574012 1:+13014219 None:intergenic
CCCGTAGCAAGAGGACTCGT+GGG 0.574146 1:-13014553 MsaT000960.1:CDS
GATTGTGGCTGAGTCAAGGT+TGG 0.578853 1:+13015384 None:intergenic
TTGGGTTGGGAAAGCTCACG+GGG 0.578980 1:+13014623 None:intergenic
CAGCTAAGGCCCGTAGCAAG+AGG 0.579929 1:-13014562 MsaT000960.1:CDS
TTAGGCCCGGTTCGCCATTG+TGG 0.587065 1:+13014323 None:intergenic
TTAGCTCGTCATCATTGTCC+GGG 0.587117 1:-13015318 MsaT000960.1:CDS
TTGTGGTGTAAGATACAAGT+CGG 0.589234 1:-13014276 MsaT000960.1:CDS
CTGCTTCCTTGGAACAGTGA+CGG 0.590093 1:+13014433 None:intergenic
ATTCCCTACGTAGGAATGGA+AGG 0.590186 1:-13015532 None:intergenic
ATTGTCGGTAGTGTTGTTGT+CGG 0.590207 1:+13015474 None:intergenic
GGTCAGGTGGAGATGGTCGG+AGG 0.590443 1:-13014373 MsaT000960.1:CDS
GTCGGTAGTGTTGTTGTCGG+AGG 0.590547 1:+13015477 None:intergenic
TACCTACCGTCACTGTTCCA+AGG 0.593877 1:-13014439 MsaT000960.1:CDS
CGTCGTCGACGTCATTGTTG+AGG 0.596242 1:+13015428 None:intergenic
CTGGATTTCCCATATTGATG+AGG 0.600285 1:-13014720 MsaT000960.1:CDS
TAACTCGCATCGTAAAGTGT+TGG 0.602752 1:-13014192 MsaT000960.1:CDS
AACGACATTAGGGTCGCACC+CGG 0.603894 1:+13015300 None:intergenic
CTCTTCCTCCACCGTTACCG+TGG 0.606184 1:-13015350 MsaT000960.1:CDS
GGCTGAGTCAAGGTTGGTGT+CGG 0.607066 1:+13015390 None:intergenic
TGTGGTGTAAGATACAAGTC+GGG 0.608929 1:-13014275 MsaT000960.1:CDS
GTTGGAGCTACGGAGGCAGA+AGG 0.611025 1:-13014174 MsaT000960.1:CDS
CGTCATTGTTGAGGTTAAGG+AGG 0.612987 1:+13015437 None:intergenic
ACGTGAGCTCCAGTCGCAAG+GGG 0.619626 1:+13014529 None:intergenic
AAAACATTATGCAACGCTTG+TGG 0.628460 1:-13014293 MsaT000960.1:CDS
GCTATTGACCACGGTAACGG+TGG 0.631846 1:+13015342 None:intergenic
GAAGAAAGGACAGTGACGGA+GGG 0.637953 1:-13014394 MsaT000960.1:CDS
CCAAGAAGAAAGGACAGTGA+CGG 0.640630 1:-13014398 MsaT000960.1:CDS
AGAAGAAAGGACAGTGACGG+AGG 0.643844 1:-13014395 MsaT000960.1:CDS
CAGTGACGGTAGGTAAGGTG+GGG 0.651207 1:+13014447 None:intergenic
AATATGGGAAATCCAGTCCA+GGG 0.656494 1:+13014727 None:intergenic
GGTGAATCAAGTAATCATCA+TGG 0.660405 1:+13014078 None:intergenic
AGAGGCGGTTGAGTTGATTG+TGG 0.662222 1:+13015369 None:intergenic
GTAAAGTGTTGGAGCTACGG+AGG 0.667005 1:-13014181 MsaT000960.1:CDS
GGAGGCAGAAGGAAATGTTG+CGG 0.675658 1:-13014163 MsaT000960.1:CDS
AGTTCTGACTCAGAAGAAGA+CGG 0.679246 1:+13014482 None:intergenic
TTCCTTGGAACAGTGACGGT+AGG 0.684302 1:+13014437 None:intergenic
GGTAACGGTGGAGGAAGAGG+CGG 0.684503 1:+13015354 None:intergenic
AGTGACGGTAGGTAAGGTGG+GGG 0.685489 1:+13014448 None:intergenic
TATAATATACCGGAACACAC+AGG 0.691414 1:+13015234 None:intergenic
CGAGCTAACGCTATTGACCA+CGG 0.719172 1:+13015333 None:intergenic
CGTGAGCTCCAGTCGCAAGG+GGG 0.723454 1:+13014530 None:intergenic
TGACAGACAAGACACCACAA+TGG 0.724676 1:-13014337 MsaT000960.1:CDS
AAACAGTCCTATATTCAACA+AGG 0.731972 1:-13014597 MsaT000960.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr1 gene 13014043 13015539 13014043 ID=MsaG000960
Chr1 mRNA 13014043 13015539 13014043 ID=MsaT000960.1;Parent=MsaG000960
Chr1 exon 13014043 13014765 13014043 ID=MsaT000960.1.exon2;Parent=MsaT000960.1
Chr1 CDS 13014043 13014765 13014043 ID=cds.MsaT000960.1;Parent=MsaT000960.1
Chr1 exon 13015231 13015539 13015231 ID=MsaT000960.1.exon1;Parent=MsaT000960.1
Chr1 CDS 13015231 13015539 13015231 ID=cds.MsaT000960.1;Parent=MsaT000960.1
Gene Sequence

>MsaT000960.1

ATGGAAGGCACAACCAACAACATTTTCCAAACCACCTTTTCCTCCGACAACAACACTACCGACAATTTCATTGTTGAGGACCTCCTTAACCTCAACAATGACGTCGACGACGACGCTACCATCATTTCCGACACCAACCTTGACTCAGCCACAATCAACTCAACCGCCTCTTCCTCCACCGTTACCGTGGTCAATAGCGTTAGCTCGTCATCATTGTCCGGGTGCGACCCTAATGTCGTTCCTGATATTGGTATCGAATTTTTACGGACCATTTCTCGAGAGACCTGTGTGTTCCGGTATATTATATGTGAGGATGACTTAGCGGCCCTGGACTGGATTTCCCATATTGATGAGGAATCGTTTTCAAGCGAGGATCTTCAAAAGATGCAACTGATATCGGGCATGAAGGTGCGAAACCAGGATGAAGAACCCCGTGAGCTTTCCCAACCCAACCGAAACAGTCCTATATTCAACAAGGACGTTTTGGTTCCAGCTAAGGCCCGTAGCAAGAGGACTCGTGGGCCCCCTTGCGACTGGAGCTCACGTCTTCTTGTTTTGTCTCAAACAACACCGTCTTCTTCTGAGTCAGAACTCCTTATTCCCACCCCCACCTTACCTACCGTCACTGTTCCAAGGAAGCAGGCAAAGACTGCTCCAAGAAGAAAGGACAGTGACGGAGGGTCAGGTGGAGATGGTCGGAGGTGTCTGCATTGCATGACAGACAAGACACCACAATGGCGAACCGGGCCTAATGGTCCGAAAACATTATGCAACGCTTGTGGTGTAAGATACAAGTCGGGTCGATTGGTGCCTGAATACAGGCCTGCAGCTAGCCCTACATTTGTGCTGACCAAACACTCTAACTCGCATCGTAAAGTGTTGGAGCTACGGAGGCAGAAGGAAATGTTGCGGGCACACCAACACCAACAAAACCAGATTTTACAACATCAGAGTATGATGTTTGATATGTCCAACCATGATGATTACTTGATTCACCAACATATGGGACCTGATTTTAGGCAGATGATGTAG

Protein sequence

>MsaT000960.1

MEGTTNNIFQTTFSSDNNTTDNFIVEDLLNLNNDVDDDATIISDTNLDSATINSTASSSTVTVVNSVSSSSLSGCDPNVVPDIGIEFLRTISRETCVFRYIICEDDLAALDWISHIDEESFSSEDLQKMQLISGMKVRNQDEEPRELSQPNRNSPIFNKDVLVPAKARSKRTRGPPCDWSSRLLVLSQTTPSSSESELLIPTPTLPTVTVPRKQAKTAPRRKDSDGGSGGDGRRCLHCMTDKTPQWRTGPNGPKTLCNACGVRYKSGRLVPEYRPAASPTFVLTKHSNSHRKVLELRRQKEMLRAHQHQQNQILQHQSMMFDMSNHDDYLIHQHMGPDFRQMM*