Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG000967 | XP_013465670.1 | 97.872 | 423 | 9 | 0 | 1 | 423 | 1 | 423 | 0.0 | 860 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG000967 | sp|Q8LPI7|CKL4_ARATH | 59.375 | 416 | 149 | 3 | 1 | 408 | 1 | 404 | 7.73e-179 | 508 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG000967 | tr|A0A072VDN0|A0A072VDN0_MEDTR | 97.872 | 423 | 9 | 0 | 1 | 423 | 1 | 423 | 0.0 | 860 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsaG000967 | MsaG037739 | 0.900008 | 2.587061e-77 | 1.324081e-73 |
| MsaG000967 | MsaG045532 | 0.864447 | 2.408214e-64 | 2.225693e-61 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG000967 | MtrunA17_Chr1g0146151 | 97.872 | 423 | 9 | 0 | 1 | 423 | 1 | 423 | 0.0 | 860 |
| MsaG000967 | MtrunA17_Chr3g0140071 | 62.617 | 428 | 153 | 2 | 1 | 421 | 1 | 428 | 0.0 | 537 |
| MsaG000967 | MtrunA17_Chr6g0486611 | 71.605 | 324 | 91 | 1 | 1 | 323 | 1 | 324 | 4.94e-177 | 501 |
| MsaG000967 | MtrunA17_Chr8g0390211 | 59.251 | 427 | 163 | 5 | 1 | 421 | 1 | 422 | 8.17e-177 | 500 |
| MsaG000967 | MtrunA17_Chr4g0071031 | 66.565 | 329 | 107 | 1 | 1 | 326 | 1 | 329 | 3.25e-166 | 474 |
| MsaG000967 | MtrunA17_Chr2g0329981 | 67.398 | 319 | 104 | 0 | 1 | 319 | 1 | 319 | 5.96e-165 | 472 |
| MsaG000967 | MtrunA17_Chr2g0299661 | 65.350 | 329 | 111 | 1 | 1 | 326 | 1 | 329 | 7.20e-164 | 469 |
| MsaG000967 | MtrunA17_Chr1g0166821 | 65.465 | 333 | 110 | 1 | 1 | 333 | 2 | 329 | 7.72e-164 | 468 |
| MsaG000967 | MtrunA17_Chr1g0183631 | 69.831 | 295 | 89 | 0 | 1 | 295 | 1 | 295 | 6.44e-162 | 463 |
| MsaG000967 | MtrunA17_Chr1g0187571 | 64.832 | 327 | 110 | 1 | 1 | 327 | 2 | 323 | 3.57e-161 | 462 |
| MsaG000967 | MtrunA17_Chr8g0342821 | 65.831 | 319 | 108 | 1 | 1 | 319 | 1 | 318 | 3.73e-160 | 459 |
| MsaG000967 | MtrunA17_Chr7g0261501 | 67.785 | 298 | 96 | 0 | 1 | 298 | 1 | 298 | 7.86e-158 | 453 |
| MsaG000967 | MtrunA17_Chr1g0167261 | 63.851 | 296 | 105 | 1 | 1 | 294 | 2 | 297 | 9.80e-141 | 405 |
| MsaG000967 | MtrunA17_Chr1g0213321 | 37.500 | 280 | 161 | 6 | 5 | 273 | 113 | 389 | 2.66e-54 | 191 |
| MsaG000967 | MtrunA17_Chr1g0146301 | 37.809 | 283 | 162 | 7 | 2 | 273 | 114 | 393 | 2.80e-54 | 191 |
| MsaG000967 | MtrunA17_Chr7g0214531 | 35.439 | 285 | 165 | 6 | 5 | 273 | 31 | 312 | 3.85e-53 | 187 |
| MsaG000967 | MtrunA17_Chr3g0140431 | 36.749 | 283 | 165 | 7 | 2 | 273 | 28 | 307 | 1.23e-52 | 185 |
| MsaG000967 | MtrunA17_Chr7g0253211 | 35.231 | 281 | 166 | 5 | 9 | 276 | 324 | 601 | 7.24e-49 | 178 |
| MsaG000967 | MtrunA17_Chr8g0340491 | 34.043 | 282 | 164 | 6 | 9 | 273 | 128 | 404 | 3.10e-46 | 169 |
| MsaG000967 | MtrunA17_Chr5g0405421 | 27.854 | 219 | 142 | 5 | 2 | 213 | 92 | 301 | 1.84e-14 | 75.5 |
| MsaG000967 | MtrunA17_Chr5g0432471 | 27.016 | 248 | 146 | 9 | 3 | 236 | 6 | 232 | 1.24e-13 | 72.4 |
| MsaG000967 | MtrunA17_Chr8g0387671 | 29.717 | 212 | 131 | 7 | 9 | 213 | 66 | 266 | 1.27e-13 | 72.4 |
| MsaG000967 | MtrunA17_Chr8g0374701 | 26.106 | 226 | 144 | 5 | 1 | 213 | 117 | 332 | 1.35e-13 | 72.8 |
| MsaG000967 | MtrunA17_Chr4g0075541 | 28.253 | 269 | 163 | 9 | 11 | 269 | 89 | 337 | 1.70e-13 | 71.6 |
| MsaG000967 | MtrunA17_Chr5g0397101 | 28.182 | 220 | 132 | 6 | 5 | 213 | 63 | 267 | 1.97e-13 | 72.0 |
| MsaG000967 | MtrunA17_Chr0c02g0489761 | 30.000 | 220 | 128 | 8 | 5 | 213 | 74 | 278 | 2.01e-13 | 72.0 |
| MsaG000967 | MtrunA17_Chr3g0107011 | 27.500 | 240 | 153 | 9 | 4 | 236 | 9 | 234 | 3.52e-13 | 71.2 |
| MsaG000967 | MtrunA17_Chr1g0203881 | 26.582 | 237 | 155 | 7 | 8 | 238 | 40 | 263 | 1.98e-12 | 68.9 |
| MsaG000967 | MtrunA17_Chr5g0428031 | 26.638 | 229 | 147 | 7 | 7 | 227 | 7 | 222 | 2.55e-12 | 68.2 |
| MsaG000967 | MtrunA17_Chr7g0274941 | 25.000 | 236 | 158 | 5 | 8 | 237 | 15 | 237 | 3.01e-12 | 67.0 |
| MsaG000967 | MtrunA17_Chr4g0047201 | 25.118 | 211 | 136 | 7 | 77 | 284 | 276 | 467 | 3.46e-12 | 68.2 |
| MsaG000967 | MtrunA17_Chr7g0276111 | 25.339 | 221 | 145 | 5 | 3 | 213 | 138 | 348 | 4.34e-12 | 67.8 |
| MsaG000967 | MtrunA17_Chr7g0244621 | 26.897 | 290 | 166 | 14 | 11 | 278 | 47 | 312 | 5.08e-12 | 67.0 |
| MsaG000967 | MtrunA17_Chr5g0402601 | 24.252 | 301 | 195 | 10 | 8 | 293 | 371 | 653 | 5.21e-12 | 67.8 |
| MsaG000967 | MtrunA17_Chr7g0245031 | 26.496 | 234 | 150 | 7 | 9 | 236 | 148 | 365 | 5.96e-12 | 67.4 |
| MsaG000967 | MtrunA17_Chr8g0345801 | 27.068 | 266 | 166 | 10 | 1 | 258 | 8 | 253 | 1.47e-11 | 64.7 |
| MsaG000967 | MtrunA17_Chr2g0304111 | 29.279 | 222 | 128 | 11 | 6 | 214 | 20 | 225 | 2.07e-11 | 65.5 |
| MsaG000967 | MtrunA17_Chr4g0033371 | 29.586 | 169 | 109 | 4 | 8 | 167 | 71 | 238 | 2.41e-11 | 65.5 |
| MsaG000967 | MtrunA17_Chr8g0385341 | 27.014 | 211 | 138 | 5 | 9 | 213 | 30 | 230 | 2.53e-11 | 65.5 |
| MsaG000967 | MtrunA17_Chr4g0072831 | 28.319 | 226 | 114 | 8 | 7 | 232 | 8 | 185 | 2.64e-11 | 64.3 |
| MsaG000967 | MtrunA17_Chr4g0045261 | 25.778 | 225 | 143 | 6 | 1 | 213 | 129 | 341 | 3.42e-11 | 65.1 |
| MsaG000967 | MtrunA17_Chr7g0267771 | 25.822 | 213 | 142 | 5 | 9 | 215 | 88 | 290 | 3.59e-11 | 64.7 |
| MsaG000967 | MtrunA17_Chr3g0140631 | 25.000 | 248 | 162 | 8 | 9 | 250 | 37 | 266 | 6.52e-11 | 63.2 |
| MsaG000967 | MtrunA17_Chr1g0201571 | 25.214 | 234 | 150 | 9 | 9 | 231 | 126 | 345 | 8.26e-11 | 63.9 |
| MsaG000967 | MtrunA17_Chr3g0127571 | 25.561 | 223 | 142 | 7 | 3 | 213 | 132 | 342 | 8.59e-11 | 63.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG000967 | AT4G28860.1 | 59.375 | 416 | 149 | 3 | 1 | 408 | 1 | 404 | 7.86e-180 | 508 |
| MsaG000967 | AT4G28880.3 | 57.656 | 418 | 165 | 4 | 1 | 413 | 1 | 411 | 3.25e-177 | 501 |
| MsaG000967 | AT4G28880.2 | 57.656 | 418 | 165 | 4 | 1 | 413 | 1 | 411 | 3.25e-177 | 501 |
| MsaG000967 | AT4G28880.1 | 57.656 | 418 | 165 | 4 | 1 | 413 | 1 | 411 | 3.25e-177 | 501 |
| MsaG000967 | AT4G28880.4 | 57.656 | 418 | 165 | 4 | 1 | 413 | 1 | 411 | 3.25e-177 | 501 |
| MsaG000967 | AT4G28860.2 | 63.934 | 366 | 120 | 2 | 1 | 366 | 1 | 354 | 8.24e-176 | 496 |
| MsaG000967 | AT4G26100.1 | 55.637 | 408 | 169 | 5 | 1 | 405 | 1 | 399 | 3.28e-166 | 474 |
| MsaG000967 | AT5G57015.1 | 67.702 | 322 | 102 | 1 | 1 | 320 | 1 | 322 | 6.57e-166 | 473 |
| MsaG000967 | AT3G23340.1 | 56.219 | 402 | 163 | 4 | 1 | 396 | 1 | 395 | 1.91e-160 | 459 |
| MsaG000967 | AT1G72710.1 | 66.144 | 319 | 107 | 1 | 1 | 319 | 1 | 318 | 4.72e-160 | 459 |
| MsaG000967 | AT5G44100.1 | 68.456 | 298 | 94 | 0 | 1 | 298 | 1 | 298 | 5.09e-159 | 457 |
| MsaG000967 | AT5G44100.2 | 68.456 | 298 | 94 | 0 | 1 | 298 | 1 | 298 | 5.09e-159 | 457 |
| MsaG000967 | AT1G03930.1 | 67.114 | 298 | 98 | 0 | 1 | 298 | 1 | 298 | 6.48e-158 | 454 |
| MsaG000967 | AT2G19470.1 | 54.912 | 397 | 164 | 3 | 1 | 397 | 1 | 382 | 1.46e-156 | 449 |
| MsaG000967 | AT4G28540.1 | 66.441 | 295 | 99 | 0 | 1 | 295 | 5 | 299 | 3.33e-155 | 447 |
| MsaG000967 | AT5G43320.1 | 67.450 | 298 | 97 | 0 | 1 | 298 | 1 | 298 | 4.61e-155 | 447 |
| MsaG000967 | AT4G14340.1 | 62.691 | 327 | 118 | 1 | 1 | 327 | 7 | 329 | 5.67e-154 | 444 |
| MsaG000967 | AT1G04440.1 | 66.779 | 298 | 99 | 0 | 1 | 298 | 1 | 298 | 1.23e-153 | 443 |
| MsaG000967 | AT4G14340.2 | 62.691 | 327 | 118 | 1 | 1 | 327 | 7 | 329 | 1.27e-153 | 439 |
| MsaG000967 | AT5G43320.2 | 67.450 | 298 | 97 | 0 | 1 | 298 | 1 | 298 | 2.14e-153 | 439 |
| MsaG000967 | AT5G43320.3 | 67.450 | 298 | 97 | 0 | 1 | 298 | 1 | 298 | 2.14e-153 | 439 |
| MsaG000967 | AT3G23340.2 | 52.985 | 402 | 162 | 5 | 1 | 396 | 1 | 381 | 4.70e-146 | 422 |
| MsaG000967 | AT4G08800.1 | 57.383 | 298 | 95 | 2 | 1 | 298 | 1 | 266 | 1.64e-123 | 360 |
| MsaG000967 | AT2G25760.2 | 36.786 | 280 | 162 | 4 | 9 | 273 | 107 | 386 | 4.10e-55 | 194 |
| MsaG000967 | AT2G25760.1 | 36.786 | 280 | 159 | 5 | 9 | 273 | 107 | 383 | 1.15e-53 | 190 |
| MsaG000967 | AT3G13670.1 | 35.640 | 289 | 167 | 6 | 5 | 277 | 131 | 416 | 1.34e-53 | 190 |
| MsaG000967 | AT3G03940.1 | 35.461 | 282 | 166 | 5 | 9 | 277 | 140 | 418 | 2.21e-50 | 181 |
| MsaG000967 | AT5G18190.2 | 35.106 | 282 | 167 | 5 | 9 | 277 | 130 | 408 | 3.59e-50 | 180 |
| MsaG000967 | AT5G18190.1 | 35.106 | 282 | 167 | 5 | 9 | 277 | 130 | 408 | 3.59e-50 | 180 |
| MsaG000967 | AT3G06030.1 | 29.412 | 221 | 127 | 7 | 8 | 213 | 67 | 273 | 1.72e-14 | 75.9 |
| MsaG000967 | AT4G36070.2 | 27.982 | 218 | 140 | 6 | 8 | 217 | 70 | 278 | 2.23e-14 | 75.5 |
| MsaG000967 | AT2G17890.1 | 28.169 | 213 | 137 | 5 | 8 | 213 | 107 | 310 | 2.49e-14 | 75.5 |
| MsaG000967 | AT4G36070.1 | 27.982 | 218 | 140 | 6 | 8 | 217 | 70 | 278 | 3.24e-14 | 75.1 |
| MsaG000967 | AT5G66210.5 | 28.302 | 212 | 136 | 5 | 9 | 213 | 62 | 264 | 3.35e-14 | 74.7 |
| MsaG000967 | AT5G66210.3 | 28.302 | 212 | 136 | 5 | 9 | 213 | 62 | 264 | 3.84e-14 | 74.7 |
| MsaG000967 | AT5G66210.6 | 28.302 | 212 | 136 | 5 | 9 | 213 | 62 | 264 | 3.86e-14 | 74.3 |
| MsaG000967 | AT5G66210.4 | 28.302 | 212 | 136 | 5 | 9 | 213 | 62 | 264 | 3.86e-14 | 74.3 |
| MsaG000967 | AT5G66210.1 | 28.302 | 212 | 136 | 5 | 9 | 213 | 62 | 264 | 5.18e-14 | 74.3 |
| MsaG000967 | AT5G66210.2 | 28.302 | 212 | 136 | 5 | 9 | 213 | 62 | 264 | 5.18e-14 | 74.3 |
| MsaG000967 | AT2G46700.1 | 27.354 | 223 | 138 | 7 | 3 | 213 | 137 | 347 | 1.02e-13 | 73.6 |
| MsaG000967 | AT5G66850.2 | 27.039 | 233 | 147 | 7 | 5 | 224 | 342 | 564 | 1.38e-13 | 73.2 |
| MsaG000967 | AT5G66850.1 | 27.039 | 233 | 147 | 7 | 5 | 224 | 342 | 564 | 1.40e-13 | 73.2 |
| MsaG000967 | AT3G49370.1 | 27.854 | 219 | 138 | 6 | 7 | 215 | 140 | 348 | 3.72e-13 | 71.6 |
| MsaG000967 | AT1G62400.1 | 28.464 | 267 | 161 | 9 | 11 | 267 | 88 | 334 | 5.51e-13 | 70.5 |
| MsaG000967 | AT5G25110.1 | 25.726 | 241 | 158 | 8 | 3 | 236 | 37 | 263 | 5.72e-13 | 70.9 |
| MsaG000967 | AT3G50530.1 | 26.009 | 223 | 145 | 5 | 1 | 213 | 140 | 352 | 9.88e-13 | 70.5 |
| MsaG000967 | AT3G53930.2 | 24.627 | 268 | 175 | 6 | 5 | 254 | 16 | 274 | 1.01e-12 | 70.5 |
| MsaG000967 | AT3G53930.1 | 24.627 | 268 | 175 | 6 | 5 | 254 | 16 | 274 | 1.02e-12 | 70.5 |
| MsaG000967 | AT4G23650.1 | 30.876 | 217 | 126 | 8 | 9 | 215 | 78 | 280 | 1.74e-12 | 69.7 |
| MsaG000967 | AT5G10930.1 | 25.620 | 242 | 158 | 8 | 3 | 236 | 6 | 233 | 1.86e-12 | 69.3 |
| MsaG000967 | AT4G14580.1 | 27.511 | 229 | 134 | 9 | 2 | 213 | 10 | 223 | 1.88e-12 | 68.9 |
| MsaG000967 | AT3G23000.1 | 26.068 | 234 | 152 | 8 | 3 | 227 | 19 | 240 | 3.79e-12 | 68.2 |
| MsaG000967 | AT2G31500.1 | 28.111 | 217 | 130 | 7 | 8 | 213 | 65 | 266 | 4.63e-12 | 68.2 |
| MsaG000967 | AT1G51660.1 | 25.882 | 255 | 156 | 10 | 12 | 255 | 82 | 314 | 5.19e-12 | 67.4 |
| MsaG000967 | AT1G01140.3 | 26.141 | 241 | 150 | 10 | 7 | 236 | 17 | 240 | 7.21e-12 | 67.4 |
| MsaG000967 | AT3G08720.1 | 25.000 | 232 | 155 | 6 | 9 | 236 | 140 | 356 | 7.63e-12 | 67.4 |
| MsaG000967 | AT3G08720.3 | 25.000 | 232 | 155 | 6 | 9 | 236 | 140 | 356 | 7.63e-12 | 67.4 |
| MsaG000967 | AT3G08720.4 | 25.000 | 232 | 155 | 6 | 9 | 236 | 140 | 356 | 7.63e-12 | 67.4 |
| MsaG000967 | AT3G08720.2 | 25.000 | 232 | 155 | 6 | 9 | 236 | 140 | 356 | 7.63e-12 | 67.4 |
| MsaG000967 | AT1G08650.2 | 27.236 | 246 | 146 | 9 | 8 | 240 | 14 | 239 | 7.77e-12 | 65.9 |
| MsaG000967 | AT1G01140.1 | 27.686 | 242 | 145 | 12 | 7 | 236 | 17 | 240 | 8.46e-12 | 67.0 |
| MsaG000967 | AT1G08650.1 | 26.446 | 242 | 153 | 7 | 8 | 240 | 14 | 239 | 8.51e-12 | 65.9 |
| MsaG000967 | AT5G24430.1 | 27.397 | 219 | 139 | 7 | 7 | 215 | 141 | 349 | 1.62e-11 | 66.6 |
| MsaG000967 | AT4G24400.1 | 25.200 | 250 | 156 | 8 | 7 | 249 | 7 | 232 | 1.86e-11 | 66.2 |
| MsaG000967 | AT3G01090.2 | 30.047 | 213 | 127 | 9 | 9 | 213 | 42 | 240 | 2.22e-11 | 66.2 |
| MsaG000967 | AT3G01090.3 | 30.047 | 213 | 127 | 9 | 9 | 213 | 19 | 217 | 2.34e-11 | 65.9 |
| MsaG000967 | AT3G01090.1 | 30.047 | 213 | 127 | 9 | 9 | 213 | 19 | 217 | 2.34e-11 | 65.9 |
| MsaG000967 | AT3G08730.1 | 25.630 | 238 | 157 | 7 | 4 | 236 | 128 | 350 | 2.42e-11 | 65.9 |
| MsaG000967 | AT3G08730.2 | 25.630 | 238 | 157 | 7 | 4 | 236 | 128 | 350 | 2.42e-11 | 65.9 |
| MsaG000967 | AT1G35670.1 | 27.586 | 232 | 149 | 7 | 9 | 234 | 26 | 244 | 2.73e-11 | 65.9 |
| MsaG000967 | AT2G17530.2 | 31.973 | 147 | 88 | 4 | 2 | 136 | 31 | 177 | 2.84e-11 | 65.1 |
| MsaG000967 | AT4G24400.2 | 25.200 | 250 | 156 | 8 | 7 | 249 | 7 | 232 | 2.87e-11 | 65.5 |
| MsaG000967 | AT2G17530.1 | 31.973 | 147 | 88 | 4 | 2 | 136 | 31 | 177 | 3.22e-11 | 65.5 |
| MsaG000967 | AT2G17530.3 | 31.973 | 147 | 88 | 4 | 2 | 136 | 31 | 177 | 3.22e-11 | 65.5 |
| MsaG000967 | AT5G21326.1 | 26.104 | 249 | 140 | 10 | 7 | 236 | 11 | 234 | 3.51e-11 | 65.1 |
| MsaG000967 | AT4G32830.1 | 26.582 | 237 | 144 | 9 | 9 | 236 | 31 | 246 | 4.04e-11 | 63.9 |
| MsaG000967 | AT1G67580.1 | 27.848 | 237 | 143 | 8 | 14 | 240 | 411 | 629 | 4.31e-11 | 65.5 |
| MsaG000967 | AT1G67580.2 | 27.848 | 237 | 143 | 8 | 14 | 240 | 411 | 629 | 4.31e-11 | 65.5 |
| MsaG000967 | AT1G01140.2 | 27.686 | 242 | 147 | 12 | 7 | 236 | 17 | 242 | 4.34e-11 | 65.1 |
| MsaG000967 | AT1G63700.1 | 26.293 | 232 | 141 | 9 | 6 | 224 | 397 | 611 | 5.07e-11 | 65.5 |
| MsaG000967 | AT1G63700.2 | 26.293 | 232 | 141 | 9 | 6 | 224 | 397 | 611 | 5.07e-11 | 65.5 |
| MsaG000967 | AT2G41860.1 | 26.415 | 212 | 140 | 6 | 8 | 213 | 53 | 254 | 8.45e-11 | 64.3 |
| MsaG000967 | AT1G12580.1 | 25.581 | 215 | 142 | 6 | 8 | 215 | 43 | 246 | 8.89e-11 | 64.3 |
Find 97 sgRNAs with CRISPR-Local
Find sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGTTCTAGTTCTTGAACTTA+TGG | 0.162372 | 1:-13100931 | MsaT000967.1:CDS |
| GAGGTTTCCACAGTCGTTTC+TGG | 0.189583 | 1:+13098045 | None:intergenic |
| TCCACAGTCGTTTCTGGTTT+TGG | 0.193952 | 1:+13098051 | None:intergenic |
| GTACCAAACCATTTCATTCT+TGG | 0.227657 | 1:+13100969 | None:intergenic |
| CATTAGACTAACCTTCTTAA+TGG | 0.235709 | 1:+13101761 | None:intergenic |
| CAGGTTTGTATGATTGATTT+TGG | 0.244306 | 1:-13100166 | MsaT000967.1:CDS |
| TTACTCCCTAACACGTCATT+AGG | 0.254485 | 1:+13098018 | None:intergenic |
| CAAACCGGATAACTTTCTTA+TGG | 0.257400 | 1:-13100304 | MsaT000967.1:CDS |
| TCATTTGGTGAAATTTATAT+TGG | 0.266055 | 1:-13101902 | MsaT000967.1:intron |
| AAGTTATATAATATTCTTAA+AGG | 0.281266 | 1:-13101113 | MsaT000967.1:CDS |
| TATAGAATTGGTAGGAAAAT+TGG | 0.283963 | 1:-13101932 | MsaT000967.1:CDS |
| GTTCTAGTTCTTGAACTTAT+GGG | 0.300638 | 1:-13100930 | MsaT000967.1:CDS |
| TTCGATCAACGACCAGATTA+TGG | 0.303765 | 1:-13099118 | MsaT000967.1:CDS |
| GCAAAGCCGCAGAGATGATT+TGG | 0.307778 | 1:-13099571 | MsaT000967.1:CDS |
| AAACCGGATAACTTTCTTAT+GGG | 0.337171 | 1:-13100303 | MsaT000967.1:CDS |
| CGTGAATTGTTCACTTCTAA+AGG | 0.340115 | 1:-13099076 | MsaT000967.1:intron |
| GTCCCCTACCGTCTAATTGA+TGG | 0.360338 | 1:+13097948 | None:intergenic |
| TCAAACCCTAATGACGTGTT+AGG | 0.365272 | 1:-13098024 | MsaT000967.1:CDS |
| GATAACTTTCTTATGGGTCT+TGG | 0.366159 | 1:-13100297 | MsaT000967.1:CDS |
| GTTGGAGGAAAGTATAGAAT+TGG | 0.371829 | 1:-13101944 | MsaT000967.1:CDS |
| GTTACAAGCATATCCCTTAC+AGG | 0.376749 | 1:-13100114 | MsaT000967.1:intron |
| TCAGGAGATTCAACAGATAA+AGG | 0.377164 | 1:-13098852 | MsaT000967.1:CDS |
| CGCTTCAAATATCCATAATC+TGG | 0.378050 | 1:+13099106 | None:intergenic |
| TAGAAAATCTTATACCTGTA+AGG | 0.385287 | 1:+13100100 | None:intergenic |
| TTAGGCATGACAGTGGAAAT+AGG | 0.394364 | 1:+13098084 | None:intergenic |
| TTGGACTTTCAAAAGGATAC+AGG | 0.395538 | 1:-13100147 | MsaT000967.1:CDS |
| AAAATTGGTAGTGGATCATT+TGG | 0.395776 | 1:-13101917 | MsaT000967.1:CDS |
| AGACTGGTAGGCACGGCAAC+TGG | 0.401062 | 1:+13098516 | None:intergenic |
| TACTCCCTAACACGTCATTA+GGG | 0.401326 | 1:+13098019 | None:intergenic |
| CACGTGTTTGTATGAAGCTC+AGG | 0.404014 | 1:-13098294 | MsaT000967.1:CDS |
| AGCAATACTCACAAAGGAAT+TGG | 0.405316 | 1:-13099693 | MsaT000967.1:intron |
| ATTCCAAGAATGAAATGGTT+TGG | 0.419642 | 1:-13100972 | MsaT000967.1:CDS |
| ATAACTTTCTTATGGGTCTT+GGG | 0.425665 | 1:-13100296 | MsaT000967.1:CDS |
| TTTGATGTTGGCCGATCAAA+TGG | 0.434639 | 1:-13100850 | MsaT000967.1:intron |
| CATGATACGCATACCTCAAC+GGG | 0.445042 | 1:+13099303 | None:intergenic |
| GTGGTATTCCAAGAATGAAA+TGG | 0.445472 | 1:-13100977 | MsaT000967.1:CDS |
| ATGCTGACTATTTATATGAC+TGG | 0.453335 | 1:-13098889 | MsaT000967.1:CDS |
| AAGTGAACAATTCACGAAAC+AGG | 0.453549 | 1:+13099083 | None:intergenic |
| GATAGAGTATTTACATTCCA+AGG | 0.456851 | 1:-13100346 | MsaT000967.1:CDS |
| TAACACGTCATTAGGGTTTG+AGG | 0.458544 | 1:+13098026 | None:intergenic |
| TCTTGGGAAGAAAGCAAATC+AGG | 0.466032 | 1:-13100280 | MsaT000967.1:intron |
| GATGGAACTCGGCGTATTGA+AGG | 0.476339 | 1:+13097970 | None:intergenic |
| CAGTCTTGAACCAGGGGATG+TGG | 0.476450 | 1:-13098499 | MsaT000967.1:CDS |
| ATCTCTGTGTAGTAATCCCT+TGG | 0.476514 | 1:+13100329 | None:intergenic |
| TGAGCTTGTGGCCATTAAGA+AGG | 0.478601 | 1:-13101772 | MsaT000967.1:intron |
| AGAGATGATTTGGAGTCTCT+TGG | 0.485541 | 1:-13099561 | MsaT000967.1:CDS |
| TTGATGGAAAAGGTTGTTGG+AGG | 0.500008 | 1:-13101959 | MsaT000967.1:CDS |
| CACATCCCCTGGTTCAAGAC+TGG | 0.508245 | 1:+13098500 | None:intergenic |
| ACAACCACAGCTACTATATG+AGG | 0.509476 | 1:-13101138 | MsaT000967.1:CDS |
| ATCAATGATTACCATTTGAT+CGG | 0.517254 | 1:+13100839 | None:intergenic |
| ATGTGGTGCTTCTGATGACT+TGG | 0.519856 | 1:-13097995 | MsaT000967.1:CDS |
| TGTTGTGACTTCTGAGCTTG+TGG | 0.519886 | 1:-13101784 | MsaT000967.1:CDS |
| AGAAAATCTTATACCTGTAA+GGG | 0.525824 | 1:+13100101 | None:intergenic |
| ATGAAATGGTTTGGTACTGA+TGG | 0.526555 | 1:-13100963 | MsaT000967.1:CDS |
| GGGATATGCTTGTAACTGAT+AGG | 0.529353 | 1:+13100121 | None:intergenic |
| GTGCCTACCAGTCTTGAACC+AGG | 0.530109 | 1:-13098507 | MsaT000967.1:CDS |
| GTATGCTCATCCACATCCCC+TGG | 0.539165 | 1:+13098489 | None:intergenic |
| GGGGATGTGGATGAGCATAC+AGG | 0.543688 | 1:-13098486 | MsaT000967.1:intron |
| ATTGGTAGGAAAATTGGTAG+TGG | 0.545412 | 1:-13101926 | MsaT000967.1:CDS |
| AGACCCATAAGAAAGTTATC+CGG | 0.547262 | 1:+13100300 | None:intergenic |
| CTTGCAGACCTTGCCAAGGA+AGG | 0.550925 | 1:+13099379 | None:intergenic |
| TATTTCCACTGTCATGCCTA+AGG | 0.553869 | 1:-13098082 | MsaT000967.1:CDS |
| TTATATAATATTCTTAAAGG+AGG | 0.558963 | 1:-13101110 | MsaT000967.1:CDS |
| ACCTGCTTTACTACTGTAGT+GGG | 0.563020 | 1:-13100893 | MsaT000967.1:CDS |
| CAAACCCTAATGACGTGTTA+GGG | 0.568940 | 1:-13098023 | MsaT000967.1:CDS |
| ATAGAGTATTTACATTCCAA+GGG | 0.572950 | 1:-13100345 | MsaT000967.1:CDS |
| CATACAAACACGTGGACGGT+CGG | 0.573243 | 1:+13098303 | None:intergenic |
| TGCCTACCAGTCTTGAACCA+GGG | 0.578146 | 1:-13098506 | MsaT000967.1:CDS |
| TTCTTTGATGACAGCTTACG+AGG | 0.582045 | 1:-13100368 | MsaT000967.1:intron |
| TTTGCCTCATATAGTAGCTG+TGG | 0.584195 | 1:+13101134 | None:intergenic |
| TCCCACTACAGTAGTAAAGC+AGG | 0.584326 | 1:+13100892 | None:intergenic |
| TGGACTTTCAAAAGGATACA+GGG | 0.587624 | 1:-13100146 | MsaT000967.1:CDS |
| GACCTGCTTTACTACTGTAG+TGG | 0.588567 | 1:-13100894 | MsaT000967.1:CDS |
| ACTACACAGAGATATCAAAC+CGG | 0.594392 | 1:-13100319 | MsaT000967.1:CDS |
| TAAAGCAGGTCATCGAGACT+CGG | 0.601579 | 1:+13100906 | None:intergenic |
| GAGGAAAGTATAGAATTGGT+AGG | 0.607818 | 1:-13101940 | MsaT000967.1:CDS |
| GCATGATACGCATACCTCAA+CGG | 0.615775 | 1:+13099302 | None:intergenic |
| TCTAATTGATGGATGGAACT+CGG | 0.618953 | 1:+13097959 | None:intergenic |
| GCCTACCAGTCTTGAACCAG+GGG | 0.620423 | 1:-13098505 | MsaT000967.1:CDS |
| CCTACCGTCTAATTGATGGA+TGG | 0.625189 | 1:+13097952 | None:intergenic |
| GCTTCATACAAACACGTGGA+CGG | 0.629471 | 1:+13098299 | None:intergenic |
| AAGTAAGAAGCAAACTCCAC+GGG | 0.633022 | 1:+13099163 | None:intergenic |
| AAAGTAAGAAGCAAACTCCA+CGG | 0.637426 | 1:+13099162 | None:intergenic |
| GCAAGTAGCAATACTCACAA+AGG | 0.640002 | 1:-13099699 | MsaT000967.1:CDS |
| TGGTTCAAGACTGGTAGGCA+CGG | 0.645835 | 1:+13098509 | None:intergenic |
| GCAGCTTGCAGACCTTGCCA+AGG | 0.651297 | 1:+13099375 | None:intergenic |
| ACGTGTTTGTATGAAGCTCA+GGG | 0.653358 | 1:-13098293 | MsaT000967.1:CDS |
| TGGTCGTTGATCGAATGTCA+AGG | 0.653518 | 1:+13099126 | None:intergenic |
| TCCCCTGGTTCAAGACTGGT+AGG | 0.656075 | 1:+13098504 | None:intergenic |
| CTGAGCTTCATACAAACACG+TGG | 0.660997 | 1:+13098295 | None:intergenic |
| ACTTTGCAAGTCATGTCCCG+TGG | 0.667670 | 1:-13099179 | MsaT000967.1:CDS |
| ATCTTCCTTAGGCATGACAG+TGG | 0.673478 | 1:+13098077 | None:intergenic |
| AAAGTTAAACACTCCCGTTG+AGG | 0.684502 | 1:-13099316 | MsaT000967.1:intron |
| TCCAAAACCAGAAACGACTG+TGG | 0.684719 | 1:-13098052 | MsaT000967.1:CDS |
| GTTGATCGAATGTCAAGGAG+CGG | 0.693721 | 1:+13099131 | None:intergenic |
| GAGACTCCAAATCATCTCTG+CGG | 0.717836 | 1:+13099565 | None:intergenic |
| GACGTGTTAGGGAGTAAATG+TGG | 0.739037 | 1:-13098012 | MsaT000967.1:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr1 | gene | 13097960 | 13101978 | 13097960 | ID=MsaG000967 |
| Chr1 | mRNA | 13097960 | 13101978 | 13097960 | ID=MsaT000967.1;Parent=MsaG000967 |
| Chr1 | exon | 13097960 | 13098124 | 13097960 | ID=MsaT000967.1.exon14;Parent=MsaT000967.1 |
| Chr1 | CDS | 13097960 | 13098124 | 13097960 | ID=cds.MsaT000967.1;Parent=MsaT000967.1 |
| Chr1 | exon | 13098246 | 13098373 | 13098246 | ID=MsaT000967.1.exon13;Parent=MsaT000967.1 |
| Chr1 | CDS | 13098246 | 13098373 | 13098246 | ID=cds.MsaT000967.1;Parent=MsaT000967.1 |
| Chr1 | exon | 13098487 | 13098541 | 13098487 | ID=MsaT000967.1.exon12;Parent=MsaT000967.1 |
| Chr1 | CDS | 13098487 | 13098541 | 13098487 | ID=cds.MsaT000967.1;Parent=MsaT000967.1 |
| Chr1 | exon | 13098835 | 13098917 | 13098835 | ID=MsaT000967.1.exon11;Parent=MsaT000967.1 |
| Chr1 | CDS | 13098835 | 13098917 | 13098835 | ID=cds.MsaT000967.1;Parent=MsaT000967.1 |
| Chr1 | exon | 13099077 | 13099203 | 13099077 | ID=MsaT000967.1.exon10;Parent=MsaT000967.1 |
| Chr1 | CDS | 13099077 | 13099203 | 13099077 | ID=cds.MsaT000967.1;Parent=MsaT000967.1 |
| Chr1 | exon | 13099317 | 13099401 | 13099317 | ID=MsaT000967.1.exon9;Parent=MsaT000967.1 |
| Chr1 | CDS | 13099317 | 13099401 | 13099317 | ID=cds.MsaT000967.1;Parent=MsaT000967.1 |
| Chr1 | exon | 13099531 | 13099594 | 13099531 | ID=MsaT000967.1.exon8;Parent=MsaT000967.1 |
| Chr1 | CDS | 13099531 | 13099594 | 13099531 | ID=cds.MsaT000967.1;Parent=MsaT000967.1 |
| Chr1 | exon | 13099694 | 13099755 | 13099694 | ID=MsaT000967.1.exon7;Parent=MsaT000967.1 |
| Chr1 | CDS | 13099694 | 13099755 | 13099694 | ID=cds.MsaT000967.1;Parent=MsaT000967.1 |
| Chr1 | exon | 13100115 | 13100185 | 13100115 | ID=MsaT000967.1.exon6;Parent=MsaT000967.1 |
| Chr1 | CDS | 13100115 | 13100185 | 13100115 | ID=cds.MsaT000967.1;Parent=MsaT000967.1 |
| Chr1 | exon | 13100281 | 13100376 | 13100281 | ID=MsaT000967.1.exon5;Parent=MsaT000967.1 |
| Chr1 | CDS | 13100281 | 13100376 | 13100281 | ID=cds.MsaT000967.1;Parent=MsaT000967.1 |
| Chr1 | exon | 13100851 | 13100999 | 13100851 | ID=MsaT000967.1.exon4;Parent=MsaT000967.1 |
| Chr1 | CDS | 13100851 | 13100999 | 13100851 | ID=cds.MsaT000967.1;Parent=MsaT000967.1 |
| Chr1 | exon | 13101108 | 13101177 | 13101108 | ID=MsaT000967.1.exon3;Parent=MsaT000967.1 |
| Chr1 | CDS | 13101108 | 13101177 | 13101108 | ID=cds.MsaT000967.1;Parent=MsaT000967.1 |
| Chr1 | exon | 13101773 | 13101813 | 13101773 | ID=MsaT000967.1.exon2;Parent=MsaT000967.1 |
| Chr1 | CDS | 13101773 | 13101813 | 13101773 | ID=cds.MsaT000967.1;Parent=MsaT000967.1 |
| Chr1 | exon | 13101903 | 13101978 | 13101903 | ID=MsaT000967.1.exon1;Parent=MsaT000967.1 |
| Chr1 | CDS | 13101903 | 13101978 | 13101903 | ID=cds.MsaT000967.1;Parent=MsaT000967.1 |
| Gene Sequence |
| Protein sequence |
