Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG001019 | XP_039685920.1 | 81.229 | 1497 | 225 | 6 | 1 | 1443 | 1 | 1495 | 0.0 | 2494 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG001019 | sp|Q9LMN8|WAK3_ARATH | 45.504 | 734 | 352 | 21 | 735 | 1443 | 31 | 741 | 0.0 | 594 |
| MsaG001019 | sp|Q9LMN8|WAK3_ARATH | 45.780 | 699 | 332 | 20 | 37 | 708 | 31 | 709 | 0.0 | 574 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG001019 | tr|A0A833XJ04|A0A833XJ04_JUGRE | 53.780 | 1402 | 601 | 23 | 35 | 1408 | 32 | 1414 | 0.0 | 1464 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsaG001019 | MsaG023415 | 0.819501 | 1.965803e-52 | 4.266770e-50 |
| MsaG001019 | MsaG026867 | 0.813279 | 4.777985e-51 | 8.845327e-49 |
| MsaG001019 | MsaG028254 | 0.803019 | 7.160478e-49 | 1.036876e-46 |
| MsaG001019 | MsaG028982 | 0.800650 | 2.182591e-48 | 2.994724e-46 |
| MsaG001019 | MsaG031267 | 0.802969 | 7.331480e-49 | 1.060403e-46 |
| MsaG001019 | MsaG034778 | 0.807982 | 6.591106e-50 | 1.072156e-47 |
| MsaG001019 | MsaG041358 | 0.835461 | 3.019661e-56 | 1.025526e-53 |
| MsaG001019 | MsaG041534 | 0.802492 | 9.184854e-49 | 1.314054e-46 |
| MsaG001019 | MsaG043226 | 0.814202 | 2.999880e-51 | 5.683756e-49 |
| MsaG001019 | MsaG043815 | 0.807519 | 8.255838e-50 | 1.328100e-47 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG001019 | MtrunA17_Chr1g0158201 | 93.562 | 730 | 46 | 1 | 714 | 1443 | 16 | 744 | 0.0 | 1408 |
| MsaG001019 | MtrunA17_Chr1g0158201 | 86.536 | 713 | 95 | 1 | 1 | 713 | 1 | 712 | 0.0 | 1289 |
| MsaG001019 | MtrunA17_Chr1g0158261 | 83.447 | 731 | 114 | 4 | 714 | 1443 | 16 | 740 | 0.0 | 1254 |
| MsaG001019 | MtrunA17_Chr1g0158261 | 84.993 | 713 | 100 | 4 | 1 | 712 | 1 | 707 | 0.0 | 1254 |
| MsaG001019 | MtrunA17_Chr1g0158251 | 79.456 | 735 | 145 | 4 | 714 | 1443 | 16 | 749 | 0.0 | 1198 |
| MsaG001019 | MtrunA17_Chr1g0158251 | 80.614 | 717 | 133 | 4 | 1 | 712 | 1 | 716 | 0.0 | 1197 |
| MsaG001019 | MtrunA17_Chr1g0148501 | 59.816 | 759 | 277 | 12 | 707 | 1443 | 9 | 761 | 0.0 | 886 |
| MsaG001019 | MtrunA17_Chr1g0148501 | 59.537 | 734 | 263 | 12 | 9 | 713 | 2 | 730 | 0.0 | 866 |
| MsaG001019 | MtrunA17_Chr1g0148511 | 60.211 | 759 | 274 | 12 | 707 | 1443 | 3 | 755 | 0.0 | 878 |
| MsaG001019 | MtrunA17_Chr1g0148511 | 60.440 | 728 | 260 | 10 | 6 | 713 | 5 | 724 | 0.0 | 863 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG001019 | AT1G21240.1 | 45.504 | 734 | 352 | 21 | 735 | 1443 | 31 | 741 | 0.0 | 594 |
| MsaG001019 | AT1G21240.1 | 45.780 | 699 | 332 | 20 | 37 | 708 | 31 | 709 | 0.0 | 574 |
| MsaG001019 | AT1G21270.1 | 45.317 | 726 | 358 | 17 | 735 | 1443 | 29 | 732 | 0.0 | 590 |
| MsaG001019 | AT1G21270.1 | 46.387 | 692 | 331 | 19 | 37 | 708 | 29 | 700 | 0.0 | 585 |
| MsaG001019 | AT1G21230.1 | 45.082 | 732 | 352 | 18 | 735 | 1443 | 29 | 733 | 0.0 | 581 |
| MsaG001019 | AT1G21230.1 | 46.454 | 691 | 334 | 15 | 37 | 708 | 29 | 702 | 0.0 | 577 |
| MsaG001019 | AT1G21250.1 | 43.699 | 730 | 370 | 18 | 733 | 1443 | 28 | 735 | 0.0 | 566 |
| MsaG001019 | AT1G21250.1 | 43.948 | 694 | 351 | 17 | 35 | 708 | 28 | 703 | 1.65e-180 | 555 |
| MsaG001019 | AT1G21210.1 | 43.225 | 738 | 371 | 20 | 731 | 1443 | 24 | 738 | 0.0 | 561 |
| MsaG001019 | AT1G21210.1 | 44.333 | 697 | 351 | 16 | 34 | 708 | 25 | 706 | 8.02e-180 | 553 |
Find 247 sgRNAs with CRISPR-Local
Find sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAAGTGATGTCTATAGCTT+TGG | 0.393895 | 1:-13972103 | MsaT001019.1:CDS |
| AAAAGTGATGTTTATAGTTT+TGG | 0.254664 | 1:-13981786 | MsaT001019.1:CDS |
| AAAAGTTGTAAGGCTTCAAA+GGG | 0.534276 | 1:-13974309 | MsaT001019.1:CDS |
| AAAATACCTATGCAACCATC+TGG | 0.445183 | 1:+13974180 | None:intergenic |
| AAACAAGGAGGAGATTAAAG+AGG | 0.579045 | 1:-13971933 | MsaT001019.1:CDS |
| AAACAAGGAGGAGATTAAGG+AGG | 0.648399 | 1:-13981616 | MsaT001019.1:CDS |
| AAATGTAAGGAAGGTTATGA+AGG | 0.545930 | 1:-13974216 | MsaT001019.1:CDS |
| AAATGTAAGGAAGGTTATGA+AGG | 0.545930 | 1:-13983998 | MsaT001019.1:CDS |
| AAATTACCTATGCAGCCATC+TGG | 0.451604 | 1:+13983962 | None:intergenic |
| AACAAAGAAACATATTCAAC+CGG | 0.459369 | 1:-13974591 | MsaT001019.1:CDS |
CRISPR-GE
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr1 | gene | 13971664 | 13984830 | 13971664 | ID=MsaG001019 |
| Chr1 | mRNA | 13971664 | 13984830 | 13971664 | ID=MsaT001019.1;Parent=MsaG001019 |
| Chr1 | exon | 13971664 | 13972877 | 13971664 | ID=MsaT001019.1.exon6;Parent=MsaT001019.1 |
| Chr1 | CDS | 13971664 | 13972877 | 13971664 | ID=cds.MsaT001019.1;Parent=MsaT001019.1 |
| Chr1 | exon | 13973199 | 13973360 | 13973199 | ID=MsaT001019.1.exon5;Parent=MsaT001019.1 |
| Chr1 | CDS | 13973199 | 13973360 | 13973199 | ID=cds.MsaT001019.1;Parent=MsaT001019.1 |
| Chr1 | exon | 13974187 | 13975002 | 13974187 | ID=MsaT001019.1.exon4;Parent=MsaT001019.1 |
| Chr1 | CDS | 13974187 | 13975002 | 13974187 | ID=cds.MsaT001019.1;Parent=MsaT001019.1 |
| Chr1 | exon | 13981448 | 13982560 | 13981448 | ID=MsaT001019.1.exon3;Parent=MsaT001019.1 |
| Chr1 | CDS | 13981448 | 13982560 | 13981448 | ID=cds.MsaT001019.1;Parent=MsaT001019.1 |
| Chr1 | exon | 13982774 | 13982938 | 13982774 | ID=MsaT001019.1.exon2;Parent=MsaT001019.1 |
| Chr1 | CDS | 13982774 | 13982938 | 13982774 | ID=cds.MsaT001019.1;Parent=MsaT001019.1 |
| Chr1 | exon | 13983969 | 13984830 | 13983969 | ID=MsaT001019.1.exon1;Parent=MsaT001019.1 |
| Chr1 | CDS | 13983969 | 13984830 | 13983969 | ID=cds.MsaT001019.1;Parent=MsaT001019.1 |
| Gene Sequence |
>MsaT001019.1 ATGGAACTGAAGACAAAGATATCACAACTATGCCAAATTTTAATTGTTACAATATCCATGTCAAGTGTATTTGCATCCGATGAGTCTCTTCCTTCATTCCTACCCGGTTGCAAAAACACATGTGGTGATGTCAAAATTCCATACCCATTTGGAATATCCAATTCATCAATACCAAACCAAGGACCATGTTTTCTAGAGCCCCAATTCAATCTTGTATGTGAAAACGACACAAAATTATTTTTGAGCAATTTACAAGTTTCCAACATAAACATCCTTGAAGGACAACTTGAATTGTCGTTTTTTGTCGGAGGTTATTGTAGTAGCACAGATTACAATCGACCCACTTTAGGCACTGCCGGTTACACCATATCAAGCAAGGAAAACAAGTTTGTAACTGTTGGTTGTGATAGTTATGGTTATCTCAACAGCATTTACAATCAAGAAACATATTCAACTGGTTGCTTAACAAGATGTAACGGTAATCGAAATCGAATCGAAAATGGAACATGTTCTGGTATTGGTTGTTGTCAAGTTGATATTCCACACATGATGAGGAATATAAGTGTACAAGCTTTTGATTTTCCTAATTCAACGGATTCTTTAGGGTGTAGTTATTCATTTGTTGTTAAAGATGGATTTTATAATTTCTCTATTGATCATTTGGATAAATTTCCTTACAAGATGCTTCCTTTGGTTCTTGATTGGAGTGTTGGAAGTGAAAATTGTAAGGCTTCAAAGGGTAAAGATGGTTATGCTTGCAAGAAGAATAGTGATTGTGATGATGAAAACATTGGTGTTGGTTACCGATGTAAATGTAAGGAAGGTTATGAAGGAAACCCATATCATCCAGATGGCTGCATAGACATCGACGAATGTAAGACTTTAAATAAGACATCATGTATAGATGAAAGGCATTGTCGCAACACGGATGGATTATATGAATGTTTCTGTCCTGATGGACAATCTGGAAATGGAACACTTGAAGGAGGTTGTCATAGACGAGATATTGTTACCAAGATTGCCATTGGAGCAAGTGCTGGAATCATTATTCTATTTGTGGCGATTTCAGTTCTATACTTGACGTACCAAAAAAGGAAATTAATCAAACTAAAGCAAAAATTCTTTCAAAAAAACGGAGGTTCTATTTTGCTACAACAGCTATCAACAAGAGAAGACACATCACAATCAGCTCAAATATTCACAGAAGAGGAACTTAAGAAAGCCACCAAAAACTACGATGATAGCTTAATCATCGGCAGAGGAGGTTTCGGTACAGTTTTCAAAGGAGTTTTACCCGACAACAAAATCGTCGCTGTAAAAAAATCCAAGATAATAGACGCAAATCAAATTGAGCAATTCATTAATGAGGTAGTTGTTCTGTCCCAGATAAATCATAGGAATGTTGTCAAACTTTTAGGGTGTTGTTTGGAGACAGAAGTTCCTTCCTTGGTGTATGAATTCGTTAGCAATGGTACACTTTTCGATTTCATACATAGTACTAAAAATAAAGCAAGCAATCCAACATGGAAAACGCGTTTAAGGATAGCAGCAGAGACAGCCGGAGCTTTATCGTATCTACATTCATCTGCATCAATACCTATTATCCATAGAGATGTTAAGAGTACCAACATTCTCTTAGATGACAATTACAATGCAAAAGTTTCCGATTTTGGAGCTTCGAGGTTGGTTCCACTTGATCAAACCGAGATAGCAACCATGGTACAAGGAACTCTTGGATACTTAGATCCGGAGTATATGCAAACACATCAATTAACAGAGAAAAGTGATGTTTATAGTTTTGGTGTAGTTCTTGCAGAGCTTCTAACAGGTGACAAACCTCTTTCTTTTAATAGGACAGAAGAGAATATAAGTCTTGCTATGCACTTTTTGTCTTGTTTAAAACAAGATAGAATCTTTGAAGCTATTCAAGTTGGTATTTTAAATGATGAAAACAAGGAGGAGATTAAGGAGGTTGCTATTCTTGCAGCAAGGTGTTTGAGACTTAGAGGAGATGAAAGGCCTAGCATGAAAGAAATGGCAATGGAGTTGGATGGAATAAGGTTAATGGAGAAGCATCCTTGGAATGATACTGAGCTTAATTTTGAGGAGAGACAACACTTACTTAAAGAGGCTTCTTCTATTACAATATCAATGGTAAGAGTATTTGCATCTGATGAATCCTTTTCTTCATCCCTACCTGGGTGCAAAAACACATGTGGAAATGTAAAAATTCCATATCCATTTGGAATATCAAATTCATCAATACCAAATCAAGGACCATGTTCCCTAGAGCGCAAATTTAATCTCAAATGTGTAAACGACACCAAATTAATTTTTGGCAATTTACAAGTTTCTAACATAAACATCCTTGAAGGACAACTTGAATCGTTGTTCTTAGTCTCGGGTTATTGTAATAGCACAAACTACATTCGATCCGGTTTAAACAGTCCTGCTTTCACCATATCAAGCAAGAAAAACAAGTTCGTAACAGTTGGTTGTGACAGTTACGGTTATCTCAACAGTGTATACAACAAAGAAACATATTCAACCGGTTGCCTGACAAGATGTAACGGTAATCGAAATCGAATCGAAAATGGAACATGTTCTGGTATTGGTTGTTGTCAAGTTGATATTCCACCTATGATGAGGAATATAAGTGTAGAAGCTTCTGATTTTCCTAATTCCACGGATTCTTTGGGGTGTAGTTATTCATTTGTTGTTAAAGATGGGTTTTATAATTTTTCTATTTCTCATTTGGATAATTTTCCTTATGAGAGGCTTCCTATGATTCTTGATTGGAGTGTTGGAAGTAAAAGTTGTAAGGCTTCAAAGGGTGAAGATGATTATGCTTGCAAGAAGAATAGTGTTTGTGATGATGAAAATATTGATTTTGGTTATCGATGTAAATGTAAGGAAGGTTATGAAGGAAACCCGTATCATCCAGATGGTTGCATAGACATTGATGAATGCAAGACATCAAATAACACATGCATAAATGAAGATCATTGTCGCAACAAGGATGGGTTTTATGAATGTTTCTGTCCTGATGGACAATCTGGAAATGGAACATTGGAAGGAGGTTGTCATAGACGAGATGTTACTACCAAGGTTGCCGTAGGGGCAGGTGCTGGACTCATTGTCATGTTTGTGACAATTTCATCTCTATACTTTACATGCCAAAAAAGGAAATTCATCAAACTAAAGCAAAAAAATTTTCAACAAAATGGTGGTTCGATTTTGCTACAACAACTATCTAAAAGAGAAGACACTTCCCAATCAGCTCAAATATTCACTGAAGAGGAACTTAAGAAAGCCACCAAAAACTACGATGAAAGCTTAATCATTGGTAGAGGAGGTTTTGGTACAGTTTTCAAAGGAGTTTTACCCGACAACAAAATCGTTGCTGTCAAAAAATCAAAAATAATAGATGCAAATCAAATCGAGCAATTTATTAACGAGGTAGTTGTCCTATCCCAAATAAATCACAGAAATGTTGTCAAACTTTTAGGATGTTGTTTAGAGACAGAAGTTCCTTCCTTGGTTTACGAATTTGTTAGCAATGGTACACTTTTTGATTTCATACATAGTACTAAAGATAAAGCAAACAATCCAACATGGAAAACCCGTTTAAAGATAGCAGCAGAGACAGCCGGAGCTTTATCGTATCTACATTCATCTGCATCAATACCGATTATCCATAGAGATGTCAAAAGTACAAACATTCTCTTGGATGACAAGTACACAGCAAAAGTTTCCGATTTTGGAGCTTCGAGGTTAGTTCCACTTGATCAAACTGAGATAGCAACAATGGTGCAAGGAACTCTTGGATACCTAGATCCAGAGTATATGCAAACTCATCAATTAACCGAGAAAAGTGATGTCTATAGCTTTGGAGTAGTGCTTGCAGAGCTTATAACAGGAGATAAACCTCTTTGTTTTAATAGGCCAGAAGAGAATATTAGTCTTGCTATGCACTTTTTGTCTTGTTTAAAACAAGATAGAATTTTTGAAGCTATTCAAGTAGGTATCTTGAATGATGAAAACAAGGAGGAGATTAAAGAGGTTGCTATTCTTGCCGCACGGTGTTTACGACTTCGAGGTGAGGAAACACCTAGCATGAAGGAAATAGCTATGGAGTTGGATGGTATAAGGTTAATGGAGAAGCATCCTTTTAATGAATCAGAGCTGAAATTTGAGGAGAGACAACACTTGCTTAAAGAGGCATCTTCTAGAATTTATAGTGAAATTGGTGATAGTATTGTTGACCTTGAATATAGTGGGTATGACAGCTTGAAAGATCAACCATTGATTGCCTTGGATGATGGAAGATGA |
| Protein sequence |
>MsaT001019.1 MELKTKISQLCQILIVTISMSSVFASDESLPSFLPGCKNTCGDVKIPYPFGISNSSIPNQGPCFLEPQFNLVCENDTKLFLSNLQVSNINILEGQLELSFFVGGYCSSTDYNRPTLGTAGYTISSKENKFVTVGCDSYGYLNSIYNQETYSTGCLTRCNGNRNRIENGTCSGIGCCQVDIPHMMRNISVQAFDFPNSTDSLGCSYSFVVKDGFYNFSIDHLDKFPYKMLPLVLDWSVGSENCKASKGKDGYACKKNSDCDDENIGVGYRCKCKEGYEGNPYHPDGCIDIDECKTLNKTSCIDERHCRNTDGLYECFCPDGQSGNGTLEGGCHRRDIVTKIAIGASAGIIILFVAISVLYLTYQKRKLIKLKQKFFQKNGGSILLQQLSTREDTSQSAQIFTEEELKKATKNYDDSLIIGRGGFGTVFKGVLPDNKIVAVKKSKIIDANQIEQFINEVVVLSQINHRNVVKLLGCCLETEVPSLVYEFVSNGTLFDFIHSTKNKASNPTWKTRLRIAAETAGALSYLHSSASIPIIHRDVKSTNILLDDNYNAKVSDFGASRLVPLDQTEIATMVQGTLGYLDPEYMQTHQLTEKSDVYSFGVVLAELLTGDKPLSFNRTEENISLAMHFLSCLKQDRIFEAIQVGILNDENKEEIKEVAILAARCLRLRGDERPSMKEMAMELDGIRLMEKHPWNDTELNFEERQHLLKEASSITISMVRVFASDESFSSSLPGCKNTCGNVKIPYPFGISNSSIPNQGPCSLERKFNLKCVNDTKLIFGNLQVSNINILEGQLESLFLVSGYCNSTNYIRSGLNSPAFTISSKKNKFVTVGCDSYGYLNSVYNKETYSTGCLTRCNGNRNRIENGTCSGIGCCQVDIPPMMRNISVEASDFPNSTDSLGCSYSFVVKDGFYNFSISHLDNFPYERLPMILDWSVGSKSCKASKGEDDYACKKNSVCDDENIDFGYRCKCKEGYEGNPYHPDGCIDIDECKTSNNTCINEDHCRNKDGFYECFCPDGQSGNGTLEGGCHRRDVTTKVAVGAGAGLIVMFVTISSLYFTCQKRKFIKLKQKNFQQNGGSILLQQLSKREDTSQSAQIFTEEELKKATKNYDESLIIGRGGFGTVFKGVLPDNKIVAVKKSKIIDANQIEQFINEVVVLSQINHRNVVKLLGCCLETEVPSLVYEFVSNGTLFDFIHSTKDKANNPTWKTRLKIAAETAGALSYLHSSASIPIIHRDVKSTNILLDDKYTAKVSDFGASRLVPLDQTEIATMVQGTLGYLDPEYMQTHQLTEKSDVYSFGVVLAELITGDKPLCFNRPEENISLAMHFLSCLKQDRIFEAIQVGILNDENKEEIKEVAILAARCLRLRGEETPSMKEIAMELDGIRLMEKHPFNESELKFEERQHLLKEASSRIYSEIGDSIVDLEYSGYDSLKDQPLIALDDGR* |
