AlfalfaGEDB | MsaG001073

M. sativa ssp. caerulea / MsaG001073

KEGG: K08065
GO: -
PFAM: PF00808.26

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG001073	XP_004498435.1	56.627	166	50	3	1	166	1	144	1.60e-53	177

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG001073	sp\|Q9SFD8\|NFYB9_ARATH	43.382	136	72	2	5	139	16	147	9.58e-34	122

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG001073	tr\|A0A1S2Y2P9\|A0A1S2Y2P9_CICAR	56.627	166	50	3	1	166	1	144	7.66e-54	177

TFs/TRs:

Gene ID	Type	Classification
MsaG001073	TF	NF-YB

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsaG005389	MsaG001073	0.802702	8.318965e-49	1.195868e-46
MsaG015250	MsaG001073	0.809425	3.250461e-50	5.474450e-48
MsaG020964	MsaG001073	0.878457	5.802215e-69	9.749967e-66
MsaG024022	MsaG001073	0.857554	2.939570e-62	2.084744e-59

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG001073	MtrunA17_Chr1g0158951	83.696	92	15	0	81	172	19	110	1.00e-53	166
MsaG001073	MtrunA17_Chr1g0165041	63.333	90	33	0	50	139	5	94	1.74e-39	132
MsaG001073	MtrunA17_Chr4g0076381	48.333	120	58	2	21	139	30	146	3.20e-35	123
MsaG001073	MtrunA17_Chr7g0263601	41.667	132	64	2	44	173	17	137	1.91e-34	119
MsaG001073	MtrunA17_Chr1g0159271	58.065	93	39	0	65	157	1	93	3.95e-34	116
MsaG001073	MtrunA17_Chr8g0382931	53.846	91	42	0	49	139	26	116	1.49e-33	117
MsaG001073	MtrunA17_Chr3g0102351	44.286	140	69	4	29	164	2	136	1.54e-33	117
MsaG001073	MtrunA17_Chr2g0290491	44.444	117	58	1	32	148	5	114	2.10e-33	116
MsaG001073	MtrunA17_Chr1g0185911	52.747	91	43	0	49	139	27	117	4.90e-33	115
MsaG001073	MtrunA17_Chr5g0446491	46.789	109	54	1	50	158	22	126	1.07e-32	115
MsaG001073	MtrunA17_Chr4g0076321	42.308	130	70	1	32	161	5	129	7.48e-32	111
MsaG001073	MtrunA17_Chr8g0384451	45.000	100	55	0	50	149	39	138	9.25e-30	108
MsaG001073	MtrunA17_Chr2g0296321	48.889	90	46	0	50	139	65	154	1.05e-27	104
MsaG001073	MtrunA17_Chr4g0067091	46.465	99	53	0	50	148	21	119	1.07e-27	103
MsaG001073	MtrunA17_Chr1g0195851	46.316	95	51	0	50	144	35	129	2.21e-27	101
MsaG001073	MtrunA17_Chr1g0159291	48.750	80	41	0	52	131	3	82	2.71e-24	90.9
MsaG001073	MtrunA17_Chr1g0191981	40.230	87	52	0	53	139	6	92	4.57e-21	83.6
MsaG001073	MtrunA17_Chr5g0446601	41.176	85	50	0	56	140	29	113	5.20e-19	80.1

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG001073	AT5G47670.2	54.945	91	41	0	49	139	27	117	6.87e-35	121
MsaG001073	AT1G21970.1	43.382	136	72	2	5	139	16	147	9.74e-35	122
MsaG001073	AT5G47670.1	54.945	91	41	0	49	139	56	146	1.18e-34	122
MsaG001073	AT5G47670.3	54.945	91	41	0	49	139	56	146	1.18e-34	122
MsaG001073	AT4G14540.1	53.846	91	42	0	49	139	19	109	1.83e-34	119
MsaG001073	AT2G38880.3	44.628	121	56	1	49	169	19	128	2.21e-33	115
MsaG001073	AT2G38880.2	44.628	121	56	1	49	169	19	128	2.82e-33	115
MsaG001073	AT2G38880.1	44.628	121	56	1	49	169	19	128	2.82e-33	115
MsaG001073	AT2G38880.5	44.628	121	56	1	49	169	19	128	2.82e-33	115
MsaG001073	AT2G38880.7	51.648	91	44	0	49	139	19	109	4.02e-33	115
MsaG001073	AT2G38880.4	51.648	91	44	0	49	139	19	109	1.82e-32	112
MsaG001073	AT5G47640.1	51.648	91	44	0	50	140	26	116	3.34e-32	114
MsaG001073	AT2G38880.11	51.648	91	44	0	49	139	19	109	3.92e-32	113
MsaG001073	AT2G38880.8	51.648	91	44	0	49	139	19	109	3.92e-32	113
MsaG001073	AT2G37060.2	44.444	117	61	2	26	139	3	118	7.91e-32	112
MsaG001073	AT2G37060.3	44.444	117	61	2	26	139	3	118	7.91e-32	112
MsaG001073	AT2G37060.1	44.444	117	61	2	26	139	3	118	7.91e-32	112
MsaG001073	AT3G53340.5	50.549	91	45	0	49	139	27	117	9.15e-32	112
MsaG001073	AT3G53340.1	50.549	91	45	0	49	139	27	117	9.15e-32	112
MsaG001073	AT3G53340.4	50.549	91	45	0	49	139	27	117	1.86e-31	110
MsaG001073	AT2G38880.9	49.474	95	48	0	49	143	19	113	1.96e-31	111
MsaG001073	AT2G38880.10	49.474	95	48	0	49	143	19	113	1.96e-31	111
MsaG001073	AT2G38880.6	49.474	95	48	0	49	143	19	113	1.96e-31	111
MsaG001073	AT3G53340.3	50.549	91	45	0	49	139	27	117	2.78e-30	108
MsaG001073	AT3G53340.2	50.549	91	45	0	49	139	27	117	2.78e-30	108
MsaG001073	AT2G13570.1	49.451	91	46	0	50	140	35	125	8.43e-30	108
MsaG001073	AT2G47810.1	47.253	91	48	0	49	139	49	139	4.15e-27	100
MsaG001073	AT1G09030.1	41.573	89	52	0	51	139	3	91	3.15e-23	89.7

Find 32 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTACAGCGAACGATTTAATA+TGG	0.243997	1:-14736763	MsaT001073.1:CDS
TTGTCGAATCCAATTGTAAT+TGG	0.273848	1:+14736647	None:intergenic
AGGTAAATGCTGCTTAGTTC+CGG	0.363825	1:+14736973	None:intergenic
AATGACATGCCAATTACAAT+TGG	0.392076	1:-14736656	MsaT001073.1:CDS
GCGTTGAACGACTAAGGTTA+AGG	0.419550	1:+14737697	None:intergenic
CCACATAGTTACGATACCTT+TGG	0.434158	1:+14736687	None:intergenic
TTGATTGCGTTGAACGACTA+AGG	0.436465	1:+14737691	None:intergenic
ATATGGGCAATGGATAGGTT+AGG	0.445811	1:-14736746	MsaT001073.1:CDS
CGTTGAACGACTAAGGTTAA+GGG	0.452450	1:+14737698	None:intergenic
CATAGTTACGATACCTTTGG+AGG	0.481766	1:+14736690	None:intergenic
CTTAAAAGTAGTATGGAGAA+TGG	0.482031	1:-14737729	None:intergenic
TACAGCGAACGATTTAATAT+GGG	0.483846	1:-14736762	MsaT001073.1:CDS
ATTTAATATGGGCAATGGAT+AGG	0.498662	1:-14736751	MsaT001073.1:CDS
ATTCTTGTCAATTGCATGAT+GGG	0.500310	1:+14736923	None:intergenic
GGATTCGACAAGGATGGATC+AGG	0.515541	1:-14736635	MsaT001073.1:CDS
AACGACTAAGGTTAAGGGAC+GGG	0.520234	1:+14737703	None:intergenic
CGTTCAACGCAATCAAGCTC+TGG	0.527907	1:-14737684	MsaT001073.1:intron
ACAATTGGATTCGACAAGGA+TGG	0.533736	1:-14736641	MsaT001073.1:CDS
GATTCTTGTCAATTGCATGA+TGG	0.545540	1:+14736922	None:intergenic
GAACGATTTAATATGGGCAA+TGG	0.552208	1:-14736756	MsaT001073.1:CDS
GGTGCTAGTGGCTCGAACAA+TGG	0.556000	1:-14736614	MsaT001073.1:CDS
TTTACCTAAAACAAAGAATG+AGG	0.564286	1:-14736957	MsaT001073.1:CDS
AAGGATGGATCAGGTGCTAG+TGG	0.605201	1:-14736626	MsaT001073.1:CDS
AACAACAATAACTTTCACAC+CGG	0.626294	1:-14736992	MsaT001073.1:CDS
TTTGGAGGTAAAAGACTAGA+GGG	0.630419	1:+14736705	None:intergenic
CATGAGCATCATCACGAGTG+AGG	0.643678	1:-14736822	MsaT001073.1:CDS
CCAAAGGTATCGTAACTATG+TGG	0.644573	1:-14736687	MsaT001073.1:CDS
CTTTGGAGGTAAAAGACTAG+AGG	0.649236	1:+14736704	None:intergenic
GAACGACTAAGGTTAAGGGA+CGG	0.658262	1:+14737702	None:intergenic
AATTACAATTGGATTCGACA+AGG	0.668467	1:-14736645	MsaT001073.1:CDS
TGCAATTGACAAGAATCATG+CGG	0.672420	1:-14736916	MsaT001073.1:CDS
TCACGAGTGAGGCAAGCGAG+CGG	0.677839	1:-14736811	MsaT001073.1:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content

Chromosome	Type	Strat	End	Strand	Name
Chr1	gene	14736592	14737739	14736592	ID=MsaG001073
Chr1	mRNA	14736592	14737739	14736592	ID=MsaT001073.1;Parent=MsaG001073
Chr1	exon	14736592	14737058	14736592	ID=MsaT001073.1.exon2;Parent=MsaT001073.1
Chr1	CDS	14736592	14737058	14736592	ID=cds.MsaT001073.1;Parent=MsaT001073.1
Chr1	exon	14737685	14737739	14737685	ID=MsaT001073.1.exon1;Parent=MsaT001073.1
Chr1	CDS	14737685	14737739	14737685	ID=cds.MsaT001073.1;Parent=MsaT001073.1

Gene Sequence

>MsaT001073.1

ATGGAGAATGGAATCCCGTCCCTTAACCTTAGTCGTTCAACGCAATCAAGCTCTGGTAATTCATCAACAAACATTGAATCTCAAAGCAACGTCGACAACAACAACAATAACTTTCACACCGGAACTAAGCAGCATTTACCTAAAACAAAGAATGAGGAGCAGCACATTCCCATCATGCAATTGACAAGAATCATGCGGCGTGCTCTTCCCTCGCATGTAAAAATCTCTGATGGTGCTAAAGAGTCAATTCAATTGTGTTTATCTGAATTCATGAGCATCATCACGAGTGAGGCAAGCGAGCGGTGCAAGATAGAGCATCGAAAGATCGTTACAGCGAACGATTTAATATGGGCAATGGATAGGTTAGGTTTTGAAGACTATGTTGGCCCTCTAGTCTTTTACCTCCAAAGGTATCGTAACTATGTGGCTCAGCTAAATGACATGCCAATTACAATTGGATTCGACAAGGATGGATCAGGTGCTAGTGGCTCGAACAATGGCAGTGACAATGTTCACCATTGA

Protein sequence

>MsaT001073.1

MENGIPSLNLSRSTQSSSGNSSTNIESQSNVDNNNNNFHTGTKQHLPKTKNEEQHIPIMQLTRIMRRALPSHVKISDGAKESIQLCLSEFMSIITSEASERCKIEHRKIVTANDLIWAMDRLGFEDYVGPLVFYLQRYRNYVAQLNDMPITIGFDKDGSGASGSNNGSDNVHH*