AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG001095

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG001095 MtrunA17_Chr1g0159271 92.523 107 7 1 66 171 1 107 1.52e-57 175
MsaG001095 MtrunA17_Chr8g0382931 45.902 122 59 2 32 152 14 129 1.57e-31 112
MsaG001095 MtrunA17_Chr1g0185911 48.333 120 51 3 57 171 34 147 1.71e-31 111
MsaG001095 MtrunA17_Chr1g0165041 55.056 89 39 1 57 144 11 99 5.01e-31 110
MsaG001095 MtrunA17_Chr7g0263601 46.018 113 49 2 57 168 31 132 2.43e-30 108
MsaG001095 MtrunA17_Chr2g0290491 53.261 92 42 1 57 147 22 113 1.02e-29 107
MsaG001095 MtrunA17_Chr5g0446491 44.531 128 59 3 39 158 3 126 3.44e-29 105
MsaG001095 MtrunA17_Chr3g0102351 53.933 89 40 1 57 144 32 120 9.09e-29 105
MsaG001095 MtrunA17_Chr4g0076381 38.971 136 81 2 9 143 16 150 3.83e-28 104
MsaG001095 MtrunA17_Chr4g0076321 52.747 91 42 1 53 142 20 110 1.23e-27 100
MsaG001095 MtrunA17_Chr1g0195851 43.119 109 61 1 37 144 21 129 1.79e-27 102
MsaG001095 MtrunA17_Chr8g0384451 51.685 89 42 1 57 144 45 133 3.57e-27 101
MsaG001095 MtrunA17_Chr1g0158951 53.659 82 37 1 87 167 24 105 5.19e-24 90.5
MsaG001095 MtrunA17_Chr4g0067091 43.564 101 56 1 57 156 27 127 7.97e-24 93.2
MsaG001095 MtrunA17_Chr2g0296321 45.652 92 49 1 53 143 67 158 3.45e-23 92.4
MsaG001095 MtrunA17_Chr1g0159291 43.750 80 44 1 53 131 3 82 1.50e-19 78.6
MsaG001095 MtrunA17_Chr1g0191981 40.230 87 51 1 57 142 9 95 2.26e-19 79.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG001095 AT4G14540.1 50.476 105 51 1 50 153 19 123 7.70e-32 112
MsaG001095 AT5G47640.1 53.261 92 42 1 57 147 32 123 7.32e-30 108
MsaG001095 AT2G38880.7 49.107 112 52 3 57 167 26 133 9.16e-30 106
MsaG001095 AT3G53340.5 40.000 140 60 3 30 168 19 135 9.40e-30 107
MsaG001095 AT3G53340.1 40.000 140 60 3 30 168 19 135 9.40e-30 107
MsaG001095 AT2G38880.3 47.788 113 54 3 57 167 26 135 1.53e-29 105
MsaG001095 AT2G37060.2 44.828 116 60 2 57 171 35 147 1.77e-29 107
MsaG001095 AT2G37060.3 44.828 116 60 2 57 171 35 147 1.77e-29 107
MsaG001095 AT2G37060.1 44.828 116 60 2 57 171 35 147 1.77e-29 107
MsaG001095 AT2G38880.2 47.788 113 54 3 57 167 26 135 1.90e-29 105
MsaG001095 AT2G38880.1 47.788 113 54 3 57 167 26 135 1.90e-29 105
MsaG001095 AT2G38880.5 47.788 113 54 3 57 167 26 135 1.90e-29 105
MsaG001095 AT1G21970.1 39.869 153 78 4 1 142 1 150 5.49e-29 107
MsaG001095 AT5G47670.2 50.000 98 47 2 57 153 34 130 6.78e-29 106
MsaG001095 AT5G47670.1 50.000 98 47 2 57 153 63 159 1.63e-28 105
MsaG001095 AT5G47670.3 50.000 98 47 2 57 153 63 159 1.63e-28 105
MsaG001095 AT2G13570.1 52.809 89 41 1 57 144 41 129 6.47e-28 103
MsaG001095 AT3G53340.4 43.243 111 50 2 30 139 19 117 1.73e-27 100
MsaG001095 AT2G38880.4 52.381 84 39 1 57 139 26 109 1.87e-27 99.8
MsaG001095 AT3G53340.3 42.241 116 54 2 30 144 19 122 3.00e-27 100
MsaG001095 AT3G53340.2 42.241 116 54 2 30 144 19 122 3.00e-27 100
MsaG001095 AT2G38880.11 52.381 84 39 1 57 139 26 109 5.60e-27 100
MsaG001095 AT2G38880.8 52.381 84 39 1 57 139 26 109 5.60e-27 100
MsaG001095 AT2G38880.9 52.381 84 39 1 57 139 26 109 1.27e-26 99.0
MsaG001095 AT2G38880.10 52.381 84 39 1 57 139 26 109 1.27e-26 99.0
MsaG001095 AT2G38880.6 52.381 84 39 1 57 139 26 109 1.27e-26 99.0
MsaG001095 AT2G47810.1 48.810 84 42 1 57 139 56 139 1.05e-24 94.4
MsaG001095 AT1G09030.1 41.284 109 61 2 51 158 2 108 1.54e-23 90.5

Find 48 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATTAATTTCATATCAATAT+TGG 0.244898 1:-15053397 None:intergenic
GCATGATTCTTGTCAAATTT+CGG 0.290312 1:-15053067 None:intergenic
CCTCATGGTTGCGATAGTTT+TGG 0.293177 1:-15053295 None:intergenic
TTAGTGGTGAAGATATAATA+TGG 0.337402 1:+15053219 MsaT001095.1:CDS
CATGGGTAGGTTAGGGTTTG+AGG 0.343933 1:+15053244 MsaT001095.1:CDS
ATATGGGCCATGGGTAGGTT+AGG 0.348171 1:+15053236 MsaT001095.1:CDS
ATTGTGTGTGTCTGATTTCA+TGG 0.410886 1:+15053145 MsaT001095.1:CDS
AACATGGATGGGTCGGGTGC+AGG 0.435928 1:+15053353 MsaT001095.1:CDS
CACAGTTCCTCATTTGTTGT+TGG 0.457296 1:-15053035 None:intergenic
ACACAATTGTGTTGTTGCCT+TGG 0.463454 1:-15053129 None:intergenic
ATGGATGGGTCGGGTGCAGG+TGG 0.484007 1:+15053356 MsaT001095.1:CDS
GAGAGTCGAAAGATCATTAG+TGG 0.486411 1:+15053203 MsaT001095.1:CDS
TATGGGCCATGGGTAGGTTA+GGG 0.487469 1:+15053237 MsaT001095.1:CDS
GGTGGCTCGAGCAGTGGTAA+TGG 0.488471 1:+15053374 MsaT001095.1:CDS
GATTTAGTCCGTTTGCAAGC+AGG 0.497822 1:+15052939 MsaT001095.1:intron
TGGATTCAACATGGATGGGT+CGG 0.500255 1:+15053346 MsaT001095.1:CDS
AGTTCCTCATTTGTTGTTGG+AGG 0.507953 1:-15053032 None:intergenic
GGATTCAACATGGATGGGTC+GGG 0.516213 1:+15053347 MsaT001095.1:CDS
ATTGCATTTCTCAACACCTC+GGG 0.516848 1:-15053177 None:intergenic
ATTTAGTCCGTTTGCAAGCA+GGG 0.519142 1:+15052940 MsaT001095.1:intron
GATTCTTGTCAAATTTCGGA+TGG 0.519326 1:-15053063 None:intergenic
TTAGGGTTTGAGGACTATGT+TGG 0.522305 1:+15053254 MsaT001095.1:CDS
CATTGCATTTCTCAACACCT+CGG 0.522409 1:-15053178 None:intergenic
TGAAGATATAATATGGGCCA+TGG 0.523399 1:+15053226 MsaT001095.1:CDS
ATGGACTCCCTGCTTGCAAA+CGG 0.525534 1:-15052947 None:intergenic
GCTCGTGGATTCAACATGGA+TGG 0.528087 1:+15053341 MsaT001095.1:CDS
CGTTCAGCACAATCAAGCTC+TGG 0.543458 1:+15051722 MsaT001095.1:CDS
TAGTGGTGAAGATATAATAT+GGG 0.544148 1:+15053220 MsaT001095.1:CDS
GGTGCAGGTGGCTCGAGCAG+TGG 0.544238 1:+15053368 MsaT001095.1:CDS
TGAACGGTTGTTGTTAAATG+AGG 0.552826 1:-15051705 None:intergenic
TCCTAAAGTAGCATGGAGCA+TGG 0.582752 1:+15051677 None:intergenic
AGTGGCACGTCAACTGCTCG+TGG 0.583180 1:+15053326 MsaT001095.1:CDS
CTCGTGGATTCAACATGGAT+GGG 0.584715 1:+15053342 MsaT001095.1:CDS
TTTACCTCCAACAACAAATG+AGG 0.591369 1:+15053028 MsaT001095.1:CDS
AACCATGAGGCTCAATTCAG+TGG 0.593599 1:+15053308 MsaT001095.1:CDS
ATTCTTGTCAAATTTCGGAT+GGG 0.595454 1:-15053062 None:intergenic
ATGAAAACAATGAATCTCCA+AGG 0.596844 1:+15052970 MsaT001095.1:CDS
TTGTGTGTGTCTGATTTCAT+GGG 0.598205 1:+15053146 MsaT001095.1:CDS
ATATAATATGGGCCATGGGT+AGG 0.601954 1:+15053231 MsaT001095.1:CDS
AACTGCTCGTGGATTCAACA+TGG 0.604751 1:+15053337 MsaT001095.1:CDS
CTCAAACCCTAACCTACCCA+TGG 0.607706 1:-15053243 None:intergenic
GAAGATATAATATGGGCCAT+GGG 0.615040 1:+15053227 MsaT001095.1:CDS
CAGAGCTTGATTGTGCTGAA+CGG 0.629066 1:-15051721 None:intergenic
TGCCACTGAATTGAGCCTCA+TGG 0.650553 1:-15053310 None:intergenic
TTCATGGGCATCATCACCCG+AGG 0.666304 1:+15053161 MsaT001095.1:CDS
GAACGGTTGTTGTTAAATGA+GGG 0.692444 1:-15051704 None:intergenic
GAAATTTGACAAGAATCATG+CGG 0.699293 1:+15053069 MsaT001095.1:CDS
CCAAAACTATCGCAACCATG+AGG 0.711644 1:+15053295 MsaT001095.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr1 gene 15051689 15053406 15051689 ID=MsaG001095
Chr1 mRNA 15051689 15053406 15051689 ID=MsaT001095.1;Parent=MsaG001095
Chr1 exon 15051689 15051743 15051689 ID=MsaT001095.1.exon1;Parent=MsaT001095.1
Chr1 CDS 15051689 15051743 15051689 ID=cds.MsaT001095.1;Parent=MsaT001095.1
Chr1 exon 15052946 15053406 15052946 ID=MsaT001095.1.exon2;Parent=MsaT001095.1
Chr1 CDS 15052946 15053406 15052946 ID=cds.MsaT001095.1;Parent=MsaT001095.1
Gene Sequence

>MsaT001095.1

ATGGAGCATGGAAGTCCCTCATTTAACAACAACCGTTCAGCACAATCAAGCTCTGTCCGTTTGCAAGCAGGGAGTCCATATGAAAACAATGAATCTCCAAGGACCAAAAACATTGATGCTGAAATTGAGAAACAAGATTTACCTCCAACAACAAATGAGGAACTGTGTCTTCCCATCCGAAATTTGACAAGAATCATGCGGCGTGCTCTTCCATCGTACGCAAAAATATCTCAAGATGCCAAGGCAACAACACAATTGTGTGTGTCTGATTTCATGGGCATCATCACCCGAGGTGTTGAGAAATGCAATGAAGAGAGTCGAAAGATCATTAGTGGTGAAGATATAATATGGGCCATGGGTAGGTTAGGGTTTGAGGACTATGTTGGTCCTCTATCTCTTCTCCTCCAAAACTATCGCAACCATGAGGCTCAATTCAGTGGCACGTCAACTGCTCGTGGATTCAACATGGATGGGTCGGGTGCAGGTGGCTCGAGCAGTGGTAATGGCCAATATTGA
Protein sequence

>MsaT001095.1

MEHGSPSFNNNRSAQSSSVRLQAGSPYENNESPRTKNIDAEIEKQDLPPTTNEELCLPIRNLTRIMRRALPSYAKISQDAKATTQLCVSDFMGIITRGVEKCNEESRKIISGEDIIWAMGRLGFEDYVGPLSLLLQNYRNHEAQFSGTSTARGFNMDGSGAGGSSSGNGQY*