AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG001114

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG001114 MtrunA17_Chr1g0159461 92.905 592 16 2 1 566 1 592 0.0 1089
MsaG001114 MtrunA17_Chr3g0125811 66.890 598 133 6 1 566 6 570 0.0 766
MsaG001114 MtrunA17_Chr5g0447691 51.408 426 199 4 143 566 125 544 9.14e-158 462
MsaG001114 MtrunA17_Chr2g0319091 41.341 537 272 4 70 565 44 578 1.80e-140 419
MsaG001114 MtrunA17_Chr3g0101141 40.228 527 287 7 47 566 37 542 4.61e-138 411
MsaG001114 MtrunA17_Chr5g0445331 46.681 452 227 7 123 566 87 532 6.38e-136 405
MsaG001114 MtrunA17_Chr4g0076091 43.578 436 232 5 137 565 114 542 6.95e-128 385
MsaG001114 MtrunA17_Chr2g0290241 42.790 423 236 4 145 566 8 425 2.57e-123 369
MsaG001114 MtrunA17_Chr7g0224271 38.684 380 201 6 206 566 307 673 3.09e-76 254
MsaG001114 MtrunA17_Chr7g0236851 40.984 366 200 6 206 565 301 656 2.77e-75 251
MsaG001114 MtrunA17_Chr6g0468561 38.482 382 193 10 206 566 302 662 2.01e-70 239
MsaG001114 MtrunA17_Chr3g0110971 35.809 377 211 8 206 566 226 587 2.02e-65 224
MsaG001114 MtrunA17_Chr7g0245601 38.122 362 209 6 206 566 445 792 1.58e-63 223
MsaG001114 MtrunA17_Chr8g0376381 36.388 371 202 11 206 566 176 522 5.47e-62 213
MsaG001114 MtrunA17_Chr4g0031721 31.771 384 257 2 187 565 300 683 8.96e-61 213
MsaG001114 MtrunA17_Chr2g0306641 36.148 379 225 10 194 566 120 487 1.10e-60 209
MsaG001114 MtrunA17_Chr8g0357601 34.066 364 221 7 214 566 197 552 1.07e-59 207
MsaG001114 MtrunA17_Chr7g0238891 30.667 375 257 2 193 566 368 740 6.25e-58 206
MsaG001114 MtrunA17_Chr4g0057891 32.055 365 226 5 215 566 165 520 2.30e-56 197
MsaG001114 MtrunA17_Chr4g0031741 32.320 362 240 4 206 565 291 649 3.37e-56 200
MsaG001114 MtrunA17_Chr4g0038761 36.313 358 207 10 215 566 100 442 4.30e-55 192
MsaG001114 MtrunA17_Chr3g0085681 28.467 411 238 4 206 566 32 436 3.00e-54 190
MsaG001114 MtrunA17_Chr4g0031731 30.411 365 249 3 204 563 259 623 1.14e-52 189
MsaG001114 MtrunA17_Chr5g0396491 26.898 461 264 8 173 566 24 478 4.14e-52 185
MsaG001114 MtrunA17_Chr4g0031701 29.315 365 253 3 204 563 259 623 5.89e-52 187
MsaG001114 MtrunA17_Chr2g0328381 29.944 354 241 3 215 565 291 640 6.40e-52 188
MsaG001114 MtrunA17_Chr4g0031711 29.494 356 246 3 215 565 377 732 9.80e-52 189
MsaG001114 MtrunA17_Chr2g0328341 29.944 354 241 3 215 565 288 637 7.73e-51 185
MsaG001114 MtrunA17_Chr4g0057291 33.590 390 232 13 191 565 284 661 8.35e-51 185
MsaG001114 MtrunA17_Chr2g0328391 28.571 378 261 5 193 565 295 668 1.20e-50 185
MsaG001114 MtrunA17_Chr5g0401131 30.667 375 241 9 206 566 121 490 5.91e-50 179
MsaG001114 MtrunA17_Chr2g0328411 27.978 361 256 3 206 565 296 653 6.45e-48 177
MsaG001114 MtrunA17_Chr4g0039661 31.006 358 232 7 214 566 208 555 2.75e-47 173
MsaG001114 MtrunA17_Chr2g0328431 28.453 362 253 5 206 565 296 653 2.90e-47 175
MsaG001114 MtrunA17_Chr3g0114841 31.948 385 219 11 208 565 131 499 1.16e-45 168
MsaG001114 MtrunA17_Chr2g0328401 27.795 331 236 1 206 536 297 624 2.99e-43 163
MsaG001114 MtrunA17_Chr1g0200401 29.397 398 237 12 197 566 78 459 4.18e-43 160
MsaG001114 MtrunA17_Chr3g0085361 27.966 354 199 4 262 566 4 350 4.19e-43 157
MsaG001114 MtrunA17_Chr2g0329591 29.073 399 250 9 191 566 63 451 8.14e-43 159
MsaG001114 MtrunA17_Chr1g0183641 32.105 380 223 14 197 565 121 476 2.04e-42 158
MsaG001114 MtrunA17_Chr2g0328361 28.451 355 220 7 215 565 290 614 2.72e-39 152
MsaG001114 MtrunA17_Chr2g0328371 31.873 251 166 2 270 519 2 248 2.97e-38 141
MsaG001114 MtrunA17_Chr5g0424131 31.694 366 229 13 215 565 139 498 4.40e-38 147
MsaG001114 MtrunA17_Chr5g0401421 25.616 406 254 7 206 565 42 445 9.27e-37 142
MsaG001114 MtrunA17_Chr4g0053901 26.434 401 250 8 209 565 68 467 1.32e-32 130
MsaG001114 MtrunA17_Chr2g0294461 25.203 369 252 7 214 566 225 585 1.92e-30 125
MsaG001114 MtrunA17_Chr4g0038851 40.845 142 83 1 426 566 328 469 2.59e-29 121
MsaG001114 MtrunA17_Chr4g0038851 33.679 193 121 2 215 406 64 250 1.16e-27 116
MsaG001114 MtrunA17_Chr2g0294471 25.068 367 252 8 214 566 219 576 1.21e-26 114
MsaG001114 MtrunA17_Chr7g0230731 27.577 388 250 14 190 566 361 728 4.80e-24 106
MsaG001114 MtrunA17_Chr4g0012071 27.441 379 250 13 195 566 191 551 7.76e-24 105
MsaG001114 MtrunA17_Chr8g0383981 28.226 372 223 16 211 565 151 495 1.35e-23 104
MsaG001114 MtrunA17_Chr7g0230771 27.835 388 249 14 190 566 364 731 1.50e-23 105
MsaG001114 MtrunA17_Chr2g0323111 27.230 213 135 3 372 565 94 305 4.20e-21 94.4
MsaG001114 MtrunA17_Chr7g0270031 24.501 351 241 11 227 566 217 554 1.89e-20 95.1
MsaG001114 MtrunA17_Chr8g0344101 23.650 389 251 16 206 566 230 600 3.68e-19 91.3
MsaG001114 MtrunA17_Chr1g0194621 26.573 286 188 9 289 566 247 518 8.73e-18 86.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG001114 AT1G21450.1 59.091 594 199 12 1 566 16 593 0.0 649
MsaG001114 AT5G48150.1 52.448 429 197 3 139 566 68 490 5.20e-158 461
MsaG001114 AT5G48150.4 52.448 429 197 3 139 566 68 490 5.20e-158 461
MsaG001114 AT5G48150.2 52.448 429 197 3 139 566 68 490 5.20e-158 461
MsaG001114 AT5G48150.3 52.448 429 197 3 139 566 68 490 5.20e-158 461
MsaG001114 AT1G50600.3 54.728 349 158 0 218 566 178 526 1.25e-140 417
MsaG001114 AT1G50600.2 54.728 349 158 0 218 566 253 601 8.75e-140 418
MsaG001114 AT1G50600.1 54.728 349 158 0 218 566 249 597 1.04e-139 418
MsaG001114 AT2G04890.1 51.969 381 174 2 186 566 42 413 1.61e-138 408
MsaG001114 AT4G17230.1 46.172 431 220 4 137 566 106 525 6.54e-129 388
MsaG001114 AT4G17230.2 46.172 431 220 4 137 566 106 525 6.54e-129 388
MsaG001114 AT5G52510.1 38.830 376 205 8 206 566 275 640 1.32e-75 252
MsaG001114 AT3G03450.1 37.568 370 208 9 206 566 190 545 2.01e-68 231
MsaG001114 AT1G66350.1 36.885 366 205 9 206 566 162 506 2.71e-65 221
MsaG001114 AT3G54220.1 38.187 364 207 7 206 565 300 649 3.13e-64 222
MsaG001114 AT1G07530.1 34.037 379 246 3 189 565 388 764 1.28e-63 223
MsaG001114 AT5G17490.1 37.151 358 202 9 215 566 176 516 5.24e-63 216
MsaG001114 AT1G14920.1 34.877 367 217 9 206 566 179 529 4.25e-59 206
MsaG001114 AT5G59450.1 29.572 514 329 11 75 566 96 598 1.21e-58 206
MsaG001114 AT5G66770.1 34.699 366 225 7 207 566 227 584 1.54e-56 200
MsaG001114 AT2G01570.1 34.584 373 210 11 206 566 231 581 6.08e-56 198
MsaG001114 AT2G29060.2 31.319 364 246 3 206 565 291 654 1.33e-54 196
MsaG001114 AT2G29060.1 31.319 364 246 3 206 565 328 691 2.02e-54 196
MsaG001114 AT3G46600.2 29.834 362 252 2 206 566 11 371 8.40e-52 182
MsaG001114 AT2G37650.2 29.745 353 245 2 215 566 351 701 1.10e-51 189
MsaG001114 AT2G37650.1 29.745 353 245 2 215 566 363 713 1.23e-51 189
MsaG001114 AT5G41920.1 34.167 360 213 10 214 566 58 400 1.40e-51 182
MsaG001114 AT3G46600.1 29.834 362 252 2 206 566 219 579 1.73e-50 183
MsaG001114 AT3G46600.3 29.834 362 252 2 206 566 187 547 2.72e-50 182
MsaG001114 AT2G29065.1 30.105 382 256 7 193 565 248 627 5.58e-50 182
MsaG001114 AT4G37650.1 29.620 395 240 10 199 566 146 529 1.10e-49 180
MsaG001114 AT1G07520.2 29.319 382 258 8 193 565 150 528 1.80e-48 177
MsaG001114 AT1G07520.4 29.319 382 258 8 193 565 150 528 1.80e-48 177
MsaG001114 AT1G07520.1 29.319 382 258 8 193 565 314 692 3.93e-48 178
MsaG001114 AT1G07520.3 29.319 382 258 8 193 565 315 693 4.01e-48 178
MsaG001114 AT3G50650.1 32.418 364 227 8 212 566 189 542 5.34e-48 176
MsaG001114 AT1G63100.1 30.633 395 240 8 193 565 246 628 3.57e-41 158
MsaG001114 AT1G63100.2 30.633 395 240 8 193 565 271 653 5.38e-41 157
MsaG001114 AT1G55580.1 29.121 364 226 8 226 566 91 445 4.64e-40 151
MsaG001114 AT4G08250.1 29.032 341 224 7 234 565 145 476 1.19e-39 151
MsaG001114 AT3G49950.1 26.611 357 231 11 230 565 61 407 1.18e-31 127
MsaG001114 AT1G50420.1 34.597 211 129 2 197 404 55 259 5.22e-31 126
MsaG001114 AT1G50420.1 37.748 151 91 2 418 566 330 479 5.15e-26 112
MsaG001114 AT3G13840.1 25.532 376 249 11 204 566 153 510 3.21e-21 97.8
MsaG001114 AT3G60630.1 24.713 348 233 10 232 566 291 622 2.05e-19 92.4
MsaG001114 AT2G45160.1 26.452 310 200 9 269 566 346 639 2.09e-19 92.4
MsaG001114 AT5G67411.1 33.166 199 120 7 316 503 2 198 3.89e-17 80.5
MsaG001114 AT4G00150.1 23.747 379 253 13 196 566 204 554 4.07e-16 82.0

Find 148 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAAGAAATCTGATTCAAAAT+TGG 0.173475 1:+15418480 None:intergenic
CAGGCAGTTGGTTTCAATTC+AGG 0.191357 1:-15418096 MsaT001114.1:CDS
TCCACAACTGTCACAAGTTT+TGG 0.256449 1:+15417477 None:intergenic
TAGAAGCAAGTGTTTGTATC+AGG 0.258688 1:+15417805 None:intergenic
CTGATAGAACCGTCTAGTTC+TGG 0.260300 1:-15418637 MsaT001114.1:CDS
TGCCAAGCTATACTCGTTAA+AGG 0.281634 1:-15418788 MsaT001114.1:CDS
TGATACAAACACTTGCTTCT+AGG 0.282847 1:-15417803 MsaT001114.1:CDS
AACAGATCGTTGCACGGATT+CGG 0.283044 1:+15417737 None:intergenic
GGAAGGACTTGCGGCTCGTT+TGG 0.307317 1:-15418039 MsaT001114.1:CDS
ACAGATCGTTGCACGGATTC+GGG 0.312714 1:+15417738 None:intergenic
GCGATTGAAAATACAAGAAT+TGG 0.316595 1:-15418416 MsaT001114.1:CDS
GCGGCTCGTTTGGCTTCATC+GGG 0.324337 1:-15418029 MsaT001114.1:CDS
AGCTAATTCAGGAGGTTCTA+TGG 0.328878 1:-15418563 MsaT001114.1:CDS
GGTATGAAGTTGCCGGAAAA+TGG 0.337471 1:-15417260 MsaT001114.1:CDS
TCTTTGACATTTGTGCTCAT+TGG 0.345552 1:+15417201 None:intergenic
CACAGCAGAGTAGTAATTAT+AGG 0.347958 1:+15417404 None:intergenic
GTACAAGATAATAGAGGAAA+TGG 0.348833 1:-15417142 MsaT001114.1:CDS
TGCGGCTCGTTTGGCTTCAT+CGG 0.352118 1:-15418030 MsaT001114.1:CDS
TTATAGGCTTCAACAAATCT+TGG 0.356310 1:+15417420 None:intergenic
CCGTCTAGTTCTGGGATATC+AGG 0.356405 1:-15418628 MsaT001114.1:CDS
TTTGGATTTATTGCTGCTAA+TGG 0.383067 1:-15417909 MsaT001114.1:CDS
TACATGCTTCTATGCTTATC+AGG 0.384672 1:+15418724 None:intergenic
CCTGATTGCTTTGAATTTGA+CGG 0.401860 1:-15418445 MsaT001114.1:CDS
CCGTCAAATTCAAAGCAATC+AGG 0.405445 1:+15418445 None:intergenic
ATGGAGGGCAAGGATGAAAA+TGG 0.406129 1:-15417241 MsaT001114.1:CDS
CACTCTTCCGAGAGAGAGCC+AGG 0.410423 1:-15417364 MsaT001114.1:CDS
TTCAATTCAGGGAGAACCTT+CGG 0.411593 1:-15418084 MsaT001114.1:CDS
CAAATGTCAAAGAAGCAATC+AGG 0.416319 1:-15417191 MsaT001114.1:CDS
ACGATGAGGAAAACATGTTT+AGG 0.418481 1:-15418354 MsaT001114.1:CDS
ACGAAACATAAGTTTCAAAC+TGG 0.420279 1:+15418530 None:intergenic
CTCACATAAGGTGGCTTCCC+TGG 0.421499 1:+15417780 None:intergenic
GGACGACAGTTGAGCATTGA+TGG 0.424496 1:+15417612 None:intergenic
AGGCAGTTGGTTTCAATTCA+GGG 0.425383 1:-15418095 MsaT001114.1:CDS
ATAGAACGGTATGAAGTTGC+CGG 0.427124 1:-15417267 MsaT001114.1:CDS
TACCAGCTCAGAGCTAATTC+AGG 0.431218 1:-15418574 MsaT001114.1:CDS
TACAAGATAATAGAGGAAAT+GGG 0.441200 1:-15417141 MsaT001114.1:CDS
TCAACAAATCTTGGGAGAAA+GGG 0.445090 1:+15417429 None:intergenic
GTTCACCACAAGAGCTTCCC+CGG 0.452377 1:+15417590 None:intergenic
TACCGTTGATACACTTTCAT+CGG 0.452419 1:+15417542 None:intergenic
ACCGTTGATACACTTTCATC+GGG 0.454201 1:+15417543 None:intergenic
GGGTTATATATTGACTTCCT+TGG 0.454975 1:+15417826 None:intergenic
TATAGGCTTCAACAAATCTT+GGG 0.456724 1:+15417421 None:intergenic
TTCAACAAATCTTGGGAGAA+AGG 0.466115 1:+15417428 None:intergenic
TATGTCATTGATTATATCTC+CGG 0.466200 1:-15418836 MsaT001114.1:CDS
TTTCAACATTCAACCTATCC+TGG 0.466892 1:+15417346 None:intergenic
TGATAGAACCGTCTAGTTCT+GGG 0.467216 1:-15418636 MsaT001114.1:CDS
TCTTAACCATGCGGCGAAGC+TGG 0.472212 1:+15417508 None:intergenic
GAGTTGGCTGATGCGTCGTA+TGG 0.473339 1:-15418814 MsaT001114.1:CDS
TCAATGCTCAACTGTCGTCC+CGG 0.473427 1:-15417609 MsaT001114.1:CDS
CAATGCTCAACTGTCGTCCC+GGG 0.477152 1:-15417608 MsaT001114.1:CDS
GCTTATCAGGAGCAAAATCA+TGG 0.483645 1:+15418737 None:intergenic
GTTGGAGGCCTAAGTATCAT+AGG 0.491231 1:-15417717 MsaT001114.1:CDS
CAGCTATGATCGATGATCTC+AGG 0.491997 1:-15418115 MsaT001114.1:CDS
GCCAAGCTATACTCGTTAAA+GGG 0.492765 1:-15418787 MsaT001114.1:CDS
TGAAAATACAAGAATTGGAG+AGG 0.493157 1:-15418411 MsaT001114.1:CDS
TCGTCCCGGGGAAGCTCTTG+TGG 0.495157 1:-15417595 MsaT001114.1:CDS
GTCTTTGACCTATGATACTT+AGG 0.498050 1:+15417709 None:intergenic
CGACTTGTTCTGAATGGGAT+TGG 0.499359 1:+15418288 None:intergenic
AAATCATGGGAGGACAAGCC+AGG 0.501458 1:+15418751 None:intergenic
TTGGCACACTCAGCAAAGTC+AGG 0.501614 1:+15418307 None:intergenic
AAGCAATCAGGGAACTCATA+AGG 0.502002 1:-15417179 MsaT001114.1:CDS
ATTGATTATATCTCCGGAGT+TGG 0.504145 1:-15418830 MsaT001114.1:CDS
TGTCATTGCGTGTGAGGGCG+AGG 0.504713 1:-15417295 MsaT001114.1:CDS
CCACCTTATGTGAGATTGAC+CGG 0.507424 1:-15417771 MsaT001114.1:CDS
CAAACACTTGCTTCTAGGCC+AGG 0.511442 1:-15417798 MsaT001114.1:CDS
TCCCTTTAACGAGTATAGCT+TGG 0.513672 1:+15418786 None:intergenic
TGATGCGACTTGTTCTGAAT+GGG 0.520418 1:+15418283 None:intergenic
AACCTATCCTGGCTCTCTCT+CGG 0.522464 1:+15417357 None:intergenic
CATCGGGCATGTGATGAAGC+TGG 0.525795 1:+15417559 None:intergenic
GGATAGAATTGCAGCCTACA+TGG 0.528367 1:-15418063 MsaT001114.1:CDS
ACACCGGTCAATCTCACATA+AGG 0.532296 1:+15417768 None:intergenic
AAACTTATGTTTCGTCTACA+AGG 0.534213 1:-15418522 MsaT001114.1:CDS
TTATAAAGCTTTGAAATGCA+AGG 0.539721 1:-15417997 MsaT001114.1:CDS
GGCTATTAAAGATGATAAGA+AGG 0.541700 1:-15417874 MsaT001114.1:CDS
GCTACGGCACGAAATTCAAA+TGG 0.544112 1:+15417657 None:intergenic
ATGATGCGACTTGTTCTGAA+TGG 0.544379 1:+15418282 None:intergenic
GTTTAGGACTGTTCAGAGCA+TGG 0.548249 1:-15418338 MsaT001114.1:CDS
TCCAAAACTTGTGACAGTTG+TGG 0.549059 1:-15417478 MsaT001114.1:CDS
GAATCCGTGCAACGATCTGT+TGG 0.549569 1:-15417735 MsaT001114.1:CDS
AGCAATGCAGATACTATTCG+AGG 0.550536 1:-15417949 MsaT001114.1:CDS
TGACAAGTACAAGATAATAG+AGG 0.550853 1:-15417148 MsaT001114.1:CDS
GTTCCTCTGTAGGTGAATCA+AGG 0.552374 1:+15418659 None:intergenic
AGGGCAAGGATGAAAATGGC+TGG 0.554676 1:-15417237 MsaT001114.1:CDS
AGAAGCAAGTGTTTGTATCA+GGG 0.558440 1:+15417806 None:intergenic
GAATGTTGAAAGGCAATGCT+TGG 0.559198 1:-15417334 MsaT001114.1:CDS
AAGGGAGCCGATGTTACACC+TGG 0.562237 1:-15418769 MsaT001114.1:CDS
CTCCTGAATTAGCTCTGAGC+TGG 0.562844 1:+15418572 None:intergenic
ACAAGATAATAGAGGAAATG+GGG 0.563828 1:-15417140 MsaT001114.1:CDS
GATCGATGATCTCAGGCAGT+TGG 0.564311 1:-15418108 MsaT001114.1:CDS
AGTTGGCTGATGCGTCGTAT+GGG 0.565392 1:-15418813 MsaT001114.1:CDS
AATTTCGTGCCGTAGCATCA+AGG 0.565456 1:-15417650 MsaT001114.1:CDS
ACTTGATTGTTGCTTCAGCA+TGG 0.566287 1:-15417089 MsaT001114.1:CDS
TTGCCGGAAAATGGAGGGCA+AGG 0.570168 1:-15417251 MsaT001114.1:CDS
GAATTGGAGAGGGCACTGCT+TGG 0.573720 1:-15418400 MsaT001114.1:CDS
AATTGAAGACCTTGATGCTA+CGG 0.573876 1:+15417641 None:intergenic
TCCGTGCAACGATCTGTTGG+AGG 0.575063 1:-15417732 MsaT001114.1:CDS
AAGTCGCATCATGACTTCAA+AGG 0.581210 1:-15418271 MsaT001114.1:CDS
CAACAAATCTTGGGAGAAAG+GGG 0.584291 1:+15417430 None:intergenic
GAAAAGCTAGCAGAAGTACT+CGG 0.588604 1:-15417684 MsaT001114.1:CDS
ATGAAGTTGCCGGAAAATGG+AGG 0.588856 1:-15417257 MsaT001114.1:CDS
ACGAGACCAGCTTCGCCGCA+TGG 0.589388 1:-15417514 MsaT001114.1:CDS
CGTCAAATTCAAAGCAATCA+GGG 0.590617 1:+15418446 None:intergenic
CTGGCTCTCTCTCGGAAGAG+TGG 0.590772 1:+15417365 None:intergenic
GTCAATGTCATTGCGTGTGA+GGG 0.592691 1:-15417300 MsaT001114.1:CDS
AAATGTCAAAGAAGCAATCA+GGG 0.593878 1:-15417190 MsaT001114.1:CDS
GGAAATTCTGTTCATGCTGA+TGG 0.594308 1:-15418607 MsaT001114.1:CDS
GCCTCCAACAGATCGTTGCA+CGG 0.595803 1:+15417731 None:intergenic
GCCCGATGAAAGTGTATCAA+CGG 0.597244 1:-15417544 MsaT001114.1:CDS
TAAATCCAAACTTAAAACAT+GGG 0.600368 1:+15417922 None:intergenic
ATAAATCCAAACTTAAAACA+TGG 0.605905 1:+15417921 None:intergenic
GGTTCTATCAGTTCCTCTGT+AGG 0.608223 1:+15418649 None:intergenic
TTGAAAGGCAATGCTTGGCG+AGG 0.610581 1:-15417329 MsaT001114.1:CDS
AGGATAGGTTGAATGTTGAA+AGG 0.611055 1:-15417344 MsaT001114.1:CDS
CCTGATATCCCAGAACTAGA+CGG 0.611349 1:+15418628 None:intergenic
TGTGCTAGCGCACTTTCAGA+AGG 0.613130 1:-15418155 MsaT001114.1:intron
CTTCCTTGATTCACCTACAG+AGG 0.616392 1:-15418662 MsaT001114.1:CDS
AAACACTTGCTTCTAGGCCA+GGG 0.617893 1:-15417797 MsaT001114.1:CDS
GCTACTAAGATTTGAATCTG+AGG 0.622598 1:+15418239 None:intergenic
TAGGCTGCAATTCTATCCGA+AGG 0.623160 1:+15418068 None:intergenic
CCGGTCAATCTCACATAAGG+TGG 0.626350 1:+15417771 None:intergenic
ATTGCAGCCTACATGGTGGA+AGG 0.627921 1:-15418056 MsaT001114.1:CDS
CAAGATAATAGAGGAAATGG+GGG 0.629220 1:-15417139 MsaT001114.1:CDS
TGAAAGGCAATGCTTGGCGA+GGG 0.630756 1:-15417328 MsaT001114.1:CDS
TACGACGCATCAGCCAACTC+CGG 0.632209 1:+15418817 None:intergenic
GAAAATACAAGAATTGGAGA+GGG 0.632436 1:-15418410 MsaT001114.1:CDS
TGTCAATGTCATTGCGTGTG+AGG 0.632541 1:-15417301 MsaT001114.1:CDS
TTCCGAGAGAGAGCCAGGAT+AGG 0.634206 1:-15417359 MsaT001114.1:CDS
CTTATCAGGAGCAAAATCAT+GGG 0.634286 1:+15418738 None:intergenic
TGAAGTTGCCGGAAAATGGA+GGG 0.635160 1:-15417256 MsaT001114.1:CDS
GCTGCTAGACGATCAGAAGA+CGG 0.639186 1:+15417969 None:intergenic
CTACATGGTGGAAGGACTTG+CGG 0.640770 1:-15418048 MsaT001114.1:CDS
CGCAAGTCCTTCCACCATGT+AGG 0.643612 1:+15418049 None:intergenic
GGACAAGCCAGGTGTAACAT+CGG 0.651711 1:+15418762 None:intergenic
TTCACCACAAGAGCTTCCCC+GGG 0.654911 1:+15417591 None:intergenic
TGCTAATGGTGCTATCTCTG+AGG 0.658374 1:-15417895 MsaT001114.1:CDS
AACAAATCTTGGGAGAAAGG+GGG 0.658411 1:+15417431 None:intergenic
ATCAGGAGCAAAATCATGGG+AGG 0.665372 1:+15418741 None:intergenic
CAGCTCAGAGCTAATTCAGG+AGG 0.666948 1:-15418571 MsaT001114.1:CDS
AATGCTCAACTGTCGTCCCG+GGG 0.671564 1:-15417607 MsaT001114.1:CDS
GATTAAGGCTCTTAACCATG+CGG 0.672068 1:+15417499 None:intergenic
CGGCTCGTTTGGCTTCATCG+GGG 0.673846 1:-15418028 MsaT001114.1:CDS
TGATGAGTGTGATGACGATG+AGG 0.676899 1:-15418368 MsaT001114.1:CDS
GAGATCATCGATCATAGCTG+AGG 0.677523 1:+15418118 None:intergenic
TAGAATTGCAGCCTACATGG+TGG 0.682524 1:-15418060 MsaT001114.1:CDS
AGGGCGAGGATAGAATAGAA+CGG 0.705742 1:-15417281 MsaT001114.1:CDS
AAGATAATAGAGGAAATGGG+GGG 0.732816 1:-15417138 MsaT001114.1:CDS
GGATTCGGGATCATCAACAC+CGG 0.740769 1:+15417752 None:intergenic
GCTAGACGATCAGAAGACGG+AGG 0.750067 1:+15417972 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr1 gene 15417083 15418857 15417083 ID=MsaG001114
Chr1 mRNA 15417083 15418857 15417083 ID=MsaT001114.1;Parent=MsaG001114
Chr1 exon 15417083 15418169 15417083 ID=MsaT001114.1.exon2;Parent=MsaT001114.1
Chr1 CDS 15417083 15418169 15417083 ID=cds.MsaT001114.1;Parent=MsaT001114.1
Chr1 exon 15418244 15418857 15418244 ID=MsaT001114.1.exon1;Parent=MsaT001114.1
Chr1 CDS 15418244 15418857 15418244 ID=cds.MsaT001114.1;Parent=MsaT001114.1
Gene Sequence

>MsaT001114.1

ATGTCATTGATTATATCTCCGGAGTTGGCTGATGCGTCGTATGGGAATGCCAAGCTATACTCGTTAAAGGGAGCCGATGTTACACCTGGCTTGTCCTCCCATGATTTTGCTCCTGATAAGCATAGAAGCATGTACATGAATAATTCTTACTCGAGTGAGAGTTACGAAAAGTACTTCCTTGATTCACCTACAGAGGAACTGATAGAACCGTCTAGTTCTGGGATATCAGGAAATTCTGTTCATGCTGATGGTGATTCTTCGTACCAGCTCAGAGCTAATTCAGGAGGTTCTATGGTTACTGATAACCAGTTTGAAACTTATGTTTCGTCTACAAGGCAGCGTGATGCATATCAGTCCAATTTTGAATCAGATTTCTTGGAAAGTGAAAGCCCTGATTGCTTTGAATTTGACGGTGATATGCGATTGAAAATACAAGAATTGGAGAGGGCACTGCTTGGTGACAATAATGATGAGTGTGATGACGATGAGGAAAACATGTTTAGGACTGTTCAGAGCATGGAGATTGATCCTGACTTTGCTGAGTGTGCCAATCCCATTCAGAACAAGTCGCATCATGACTTCAAAGGAATCTTTGTCCTCAGATTCAAATCTTACGCACTTTCAGAAGGAAATGAAGTAAAAGCCTCAGCTATGATCGATGATCTCAGGCAGTTGGTTTCAATTCAGGGAGAACCTTCGGATAGAATTGCAGCCTACATGGTGGAAGGACTTGCGGCTCGTTTGGCTTCATCGGGGAAATGTATTTATAAAGCTTTGAAATGCAAGGAACCTCCGTCTTCTGATCGTCTAGCAGCAATGCAGATACTATTCGAGGTGTGCCCATGTTTTAAGTTTGGATTTATTGCTGCTAATGGTGCTATCTCTGAGGCTATTAAAGATGATAAGAAGGTTCACATTATAGATTTTGATATCAACCAAGGAAGTCAATATATAACCCTGATACAAACACTTGCTTCTAGGCCAGGGAAGCCACCTTATGTGAGATTGACCGGTGTTGATGATCCCGAATCCGTGCAACGATCTGTTGGAGGCCTAAGTATCATAGGTCAAAGACTTGAAAAGCTAGCAGAAGTACTCGGATTGCCATTTGAATTTCGTGCCGTAGCATCAAGGTCTTCAATTGTTACTCCATCAATGCTCAACTGTCGTCCCGGGGAAGCTCTTGTGGTGAACTTTGCATTCCAGCTTCATCACATGCCCGATGAAAGTGTATCAACGGTAAACGAACGAGACCAGCTTCGCCGCATGGTTAAGAGCCTTAATCCAAAACTTGTGACAGTTGTGGAACAAGATGTGAACACCAACACTTCCCCCTTTCTCCCAAGATTTGTTGAAGCCTATAATTACTACTCTGCTGTGTATGAGTCACTCGACGCCACTCTTCCGAGAGAGAGCCAGGATAGGTTGAATGTTGAAAGGCAATGCTTGGCGAGGGACATTGTCAATGTCATTGCGTGTGAGGGCGAGGATAGAATAGAACGGTATGAAGTTGCCGGAAAATGGAGGGCAAGGATGAAAATGGCTGGATTCACTTCATCACCAATGAGCACAAATGTCAAAGAAGCAATCAGGGAACTCATAAGGCAGTACTGTGACAAGTACAAGATAATAGAGGAAATGGGGGGACTTCATTTTGGGTGGGAAGACAAAAACTTGATTGTTGCTTCAGCATGGAAGTGA
Protein sequence

>MsaT001114.1

MSLIISPELADASYGNAKLYSLKGADVTPGLSSHDFAPDKHRSMYMNNSYSSESYEKYFLDSPTEELIEPSSSGISGNSVHADGDSSYQLRANSGGSMVTDNQFETYVSSTRQRDAYQSNFESDFLESESPDCFEFDGDMRLKIQELERALLGDNNDECDDDEENMFRTVQSMEIDPDFAECANPIQNKSHHDFKGIFVLRFKSYALSEGNEVKASAMIDDLRQLVSIQGEPSDRIAAYMVEGLAARLASSGKCIYKALKCKEPPSSDRLAAMQILFEVCPCFKFGFIAANGAISEAIKDDKKVHIIDFDINQGSQYITLIQTLASRPGKPPYVRLTGVDDPESVQRSVGGLSIIGQRLEKLAEVLGLPFEFRAVASRSSIVTPSMLNCRPGEALVVNFAFQLHHMPDESVSTVNERDQLRRMVKSLNPKLVTVVEQDVNTNTSPFLPRFVEAYNYYSAVYESLDATLPRESQDRLNVERQCLARDIVNVIACEGEDRIERYEVAGKWRARMKMAGFTSSPMSTNVKEAIRELIRQYCDKYKIIEEMGGLHFGWEDKNLIVASAWK*