AlfalfaGEDB | MsaG001149

M. sativa ssp. caerulea / MsaG001149

KEGG: K10523
GO: GO:0000151 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006508 GO:0006511 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009987 GO:0010498 GO:0019005 GO:0019222 GO:0019538 GO:0019899 GO:0019941 GO:0030162 GO:0030163 GO:0031461 GO:0031463 GO:0031625 GO:0032991 GO:0043161 GO:0043170 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044389 GO:0044424 GO:0044464 GO:0050789 GO:0051171 GO:0051246 GO:0051603 GO:0060255 GO:0065007 GO:0071704 GO:0080090 GO:1901564 GO:1901565 GO:1901575 GO:1902494 GO:1990234
PFAM: PF00651.34

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG001149	XP_013466478.1	98.476	328	5	0	1	328	1	328	0.0	674

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG001149	sp\|Q9XHZ8\|Y1178_ARATH	71.341	328	90	1	1	328	1	324	0.0	506

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG001149	tr\|A0A072VES8\|A0A072VES8_MEDTR	98.476	328	5	0	1	328	1	328	0.0	674

TFs/TRs:

Gene ID	Type	Classification
MsaG001149	TR	TRAF

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsaG001149	MsaG003753	0.809292	3.469847e-50	5.824807e-48
MsaG001149	MsaG018843	0.812153	8.404526e-51	1.512865e-48
MsaG001149	MsaG031021	0.808517	5.074851e-50	8.361959e-48
MsaG001149	MsaG042178	0.823761	2.057874e-53	5.004978e-51
MsaG001149	MsaG042138	0.813886	3.518714e-51	6.614265e-49
MsaG001149	MsaG046964	0.810272	2.140868e-50	3.680272e-48
MsaG001149	MsaG032545	0.801655	1.363053e-48	1.913160e-46

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG001149	MtrunA17_Chr1g0159831	97.879	330	5	1	1	328	1	330	0.0	669
MsaG001149	MtrunA17_Chr3g0124921	72.561	328	83	4	1	326	1	323	5.94e-176	490
MsaG001149	MtrunA17_Chr3g0103031	50.453	331	158	3	1	328	1	328	8.23e-119	345
MsaG001149	MtrunA17_Chr5g0447111	44.308	325	176	3	5	328	9	329	5.03e-98	292
MsaG001149	MtrunA17_Chr5g0447191	39.572	187	109	2	140	324	3	187	1.43e-41	143
MsaG001149	MtrunA17_Chr4g0029841	30.723	166	107	2	160	325	122	279	9.21e-21	90.5
MsaG001149	MtrunA17_Chr7g0259501	29.448	163	102	3	142	291	182	344	4.82e-11	63.5

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG001149	AT1G21780.2	71.341	328	90	1	1	328	1	324	0.0	506
MsaG001149	AT1G21780.1	71.341	328	90	1	1	328	1	324	0.0	506
MsaG001149	AT1G55760.1	47.590	332	165	5	1	328	1	327	7.04e-109	320
MsaG001149	AT2G39760.4	30.719	153	95	1	150	291	179	331	2.82e-13	70.1
MsaG001149	AT2G39760.3	30.719	153	95	1	150	291	179	331	2.82e-13	70.1
MsaG001149	AT2G39760.2	30.719	153	95	1	150	291	179	331	3.05e-13	70.1
MsaG001149	AT2G39760.1	30.719	153	95	1	150	291	179	331	6.61e-13	69.3

Find 71 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ACCCTTTCAAACTTGGTATT+TGG	0.128764	1:+15990035	MsaT001149.1:CDS
CGCAACCTCTCCCGTGTTTC+AGG	0.157581	1:+15993209	MsaT001149.1:CDS
CAATAAAACCCGAAGATTTC+TGG	0.200729	1:+15993334	MsaT001149.1:CDS
TTCAAGAAGTGCTTACAATT+TGG	0.210270	1:+15993616	MsaT001149.1:CDS
AGTGCTTACAATTTGGAAAT+AGG	0.211006	1:+15993623	MsaT001149.1:CDS
TTCGCATTCTTATTTGATTT+CGG	0.290853	1:+15993519	MsaT001149.1:CDS
TGAACCTTCGCGTGTTTCTA+AGG	0.294946	1:+15990193	MsaT001149.1:CDS
CTGTGCTTCCAGAAATCTTC+GGG	0.311859	1:-15993342	None:intergenic
TTAGAAACACGCGAAGGTTC+AGG	0.373550	1:-15990191	None:intergenic
AGAAGAAGAAGAAGCAGTAT+TGG	0.384918	1:-15990252	None:intergenic
AAGCACAGGCTGGCTTTGCT+TGG	0.389834	1:+15993357	MsaT001149.1:CDS
TCAAACTTGGTATTTGGAAC+TGG	0.400132	1:+15990041	MsaT001149.1:CDS
CAATTTCCCGATTAGCTCAA+TGG	0.401221	1:+15989972	MsaT001149.1:CDS
AAATCAGACCCTTTCAAACT+TGG	0.408826	1:+15990028	MsaT001149.1:CDS
CGAAGAATGAAACGAGCAAT+AGG	0.409761	1:-15990224	None:intergenic
CCTGTGCTTCCAGAAATCTT+CGG	0.410951	1:-15993343	None:intergenic
CTTTCTTCAACTTGTGCAGC+TGG	0.413291	1:-15993491	None:intergenic
ATTTGTCGATAGAGAGAAAC+AGG	0.424241	1:+15990147	MsaT001149.1:CDS
GAACCTGTGATGACTTTGTC+TGG	0.430022	1:+15992674	MsaT001149.1:CDS
AGGGGAAATGTAAAATTTGC+GGG	0.434591	1:-15990279	None:intergenic
TGATACGAGGCCTGAAACAC+GGG	0.458672	1:-15993219	None:intergenic
AACCGCAGATGGCACACTTA+AGG	0.459877	1:+15993167	MsaT001149.1:CDS
TTCCAAATACCAAGTTTGAA+AGG	0.463827	1:-15990037	None:intergenic
TCCAAATACCAAGTTTGAAA+GGG	0.471833	1:-15990036	None:intergenic
ATCAGATGGCCATATGGAAC+TGG	0.471880	1:-15993055	None:intergenic
CTGAAACACGGGAGAGGTTG+CGG	0.479916	1:-15993208	None:intergenic
TCGAGAGGCTTAATGAGGCC+TGG	0.483089	1:+15993463	MsaT001149.1:CDS
CCTGTGGATACTACATTCCT+TGG	0.490016	1:+15992697	MsaT001149.1:CDS
GTATCTCAGAAGGGCCGTGC+AGG	0.498716	1:-15993301	None:intergenic
CCGAGTTAAGATCTTCAAGT+AGG	0.498960	1:-15993431	None:intergenic
AGATTTCTGGAAGCACAGGC+TGG	0.511646	1:+15993347	MsaT001149.1:CDS
CAATGGAAAATCGACAACTT+TGG	0.515182	1:+15989989	MsaT001149.1:CDS
CTTGTGACATGTCTTCTATG+TGG	0.517852	1:-15993275	None:intergenic
GTAATACTACAGTTCATGAA+AGG	0.521613	1:+15992644	MsaT001149.1:intron
CAGGGGAAATGTAAAATTTG+CGG	0.522009	1:-15990280	None:intergenic
CCTACTTGAAGATCTTAACT+CGG	0.524632	1:+15993431	MsaT001149.1:CDS
CCGAAGATTTCTGGAAGCAC+AGG	0.532000	1:+15993343	MsaT001149.1:CDS
TTCCATACATGTATCTCAGA+AGG	0.534119	1:-15993311	None:intergenic
GTGGAGACGCCAGTTCCATA+TGG	0.542653	1:+15993046	MsaT001149.1:CDS
CAGCTGCACAAGTTGAAGAA+AGG	0.554405	1:+15993492	MsaT001149.1:CDS
GCCCTTCTGAGATACATGTA+TGG	0.555410	1:+15993309	MsaT001149.1:CDS
AGTTCCATATGGCCATCTGA+TGG	0.557376	1:+15993057	MsaT001149.1:CDS
ACAAAACATGGGAGATTCGA+AGG	0.560639	1:+15989943	None:intergenic
CAAGCATACGAGAGAGGCAT+CGG	0.563620	1:-15993113	None:intergenic
TAATACTACAGTTCATGAAA+GGG	0.564315	1:+15992645	MsaT001149.1:intron
TCTGATTTCTTGTACGAACA+AGG	0.568056	1:-15990013	None:intergenic
TGATGACTTTGTCTGGCCTG+TGG	0.574666	1:+15992681	MsaT001149.1:CDS
AATTAGAGACTCACATTCAG+AGG	0.576553	1:-15992763	None:intergenic
AGAGACTCACATTCAGAGGA+TGG	0.576688	1:-15992759	None:intergenic
CTCACTATTATAACCGCAGA+TGG	0.582345	1:+15993156	MsaT001149.1:CDS
CATTCAGAGGATGGATCTTG+AGG	0.589078	1:-15992750	None:intergenic
CATCAATAATCAAGCGACCA+AGG	0.591316	1:-15992714	None:intergenic
CTTGTGCAGCTGGTACAACC+AGG	0.594842	1:-15993481	None:intergenic
CTACTTGAAGATCTTAACTC+GGG	0.596940	1:+15993432	MsaT001149.1:CDS
CCAAGGAATGTAGTATCCAC+AGG	0.598731	1:-15992697	None:intergenic
GTGATACGAGGCCTGAAACA+CGG	0.601951	1:-15993220	None:intergenic
TGTTCTCGAGAGGCTTAATG+AGG	0.608295	1:+15993458	MsaT001149.1:CDS
TTCACTGCTGCTGTGATACG+AGG	0.620310	1:-15993232	None:intergenic
AGGCCAGACAAAGTCATCAC+AGG	0.625083	1:-15992677	None:intergenic
CGAGGCCTGAAACACGGGAG+AGG	0.626352	1:-15993214	None:intergenic
AGCCTTAAGTGTGCCATCTG+CGG	0.628227	1:-15993169	None:intergenic
GCAGCTAATAAGTACGACAT+TGG	0.628303	1:+15993381	MsaT001149.1:CDS
CTTTGTCAAGCATACGAGAG+AGG	0.628419	1:-15993119	None:intergenic
AGAATGAAACGAGCAATAGG+AGG	0.635724	1:-15990221	None:intergenic
TCCATACATGTATCTCAGAA+GGG	0.640688	1:-15993310	None:intergenic
ACTCGGGAAATGTTCTCGAG+AGG	0.641157	1:+15993448	MsaT001149.1:CDS
TGATCTCAAAGACGCATGCG+AGG	0.666719	1:+15993404	MsaT001149.1:CDS
CATCTGCGGTTATAATAGTG+AGG	0.691773	1:-15993155	None:intergenic
CATGTCACAAGAATCCTGCA+CGG	0.721067	1:+15993287	MsaT001149.1:CDS
GGAAATGTAAAATTTGCGGG+AGG	0.721522	1:-15990276	None:intergenic
TGTTCCTTAGAAACACGCGA+AGG	0.729696	1:-15990197	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content

Chromosome	Type	Strat	End	Strand	Name
Chr1	gene	15989950	15993644	15989950	ID=MsaG001149
Chr1	mRNA	15989950	15993644	15989950	ID=MsaT001149.1;Parent=MsaG001149
Chr1	exon	15989950	15990062	15989950	ID=MsaT001149.1.exon1;Parent=MsaT001149.1
Chr1	CDS	15989950	15990062	15989950	ID=cds.MsaT001149.1;Parent=MsaT001149.1
Chr1	exon	15990145	15990302	15990145	ID=MsaT001149.1.exon2;Parent=MsaT001149.1
Chr1	CDS	15990145	15990302	15990145	ID=cds.MsaT001149.1;Parent=MsaT001149.1
Chr1	exon	15992656	15992771	15992656	ID=MsaT001149.1.exon3;Parent=MsaT001149.1
Chr1	CDS	15992656	15992771	15992656	ID=cds.MsaT001149.1;Parent=MsaT001149.1
Chr1	exon	15993045	15993644	15993045	ID=MsaT001149.1.exon4;Parent=MsaT001149.1
Chr1	CDS	15993045	15993644	15993045	ID=cds.MsaT001149.1;Parent=MsaT001149.1

Gene Sequence

>MsaT001149.1

ATGGGAGATTCGAAGGTAGAAACAATTTCCCGATTAGCTCAATGGAAAATCGACAACTTTGGTCCTTGTTCGTACAAGAAATCAGACCCTTTCAAACTTGGTATTTGGAACTGGTATTTGTCGATAGAGAGAAACAGGTATTTGTATATTCATCTATTTCCTGAACCTTCGCGTGTTTCTAAGGAACAGCCTCCTATTGCTCGTTTCATTCTTCGTGTTTCCAATACTGCTTCTTCTTCTTCTTCCTCCCGCAAATTTTACATTTCCCCTGTTCATGAAAGGGTGCTTAGAACCTGTGATGACTTTGTCTGGCCTGTGGATACTACATTCCTTGGTCGCTTGATTATTGATGTTGAATTTCTTGACCTCAAGATCCATCCTCTGAATGGTGGAGACGCCAGTTCCATATGGCCATCTGATGGAAAACTGCATTCTGTTGCAACTCAAAACACTCTCCGATGCCTCTCTCGTATGCTTGACAAAGCTATACATGTTGACCTCACTATTATAACCGCAGATGGCACACTTAAGGCTCATAAAGCAGTTATGTCCGCAACCTCTCCCGTGTTTCAGGCCTCGTATCACAGCAGCAGTGAAGACAAAGAGTCGTCTACAATCCACATAGAAGACATGTCACAAGAATCCTGCACGGCCCTTCTGAGATACATGTATGGAACAATAAAACCCGAAGATTTCTGGAAGCACAGGCTGGCTTTGCTTGGAGCAGCTAATAAGTACGACATTGGTGATCTCAAAGACGCATGCGAGGAAAGCCTACTTGAAGATCTTAACTCGGGAAATGTTCTCGAGAGGCTTAATGAGGCCTGGTTGTACCAGCTGCACAAGTTGAAGAAAGGATGCTTCGCATTCTTATTTGATTTCGGTAAGATCTATGATGTTAGAGATGAAATAAATACGTTTTTTCGTCACGCAGACAGGGAACTCATGCTAGAGATGTTTCAAGAAGTGCTTACAATTTGGAAATAG

Protein sequence

>MsaT001149.1

MGDSKVETISRLAQWKIDNFGPCSYKKSDPFKLGIWNWYLSIERNRYLYIHLFPEPSRVSKEQPPIARFILRVSNTASSSSSSRKFYISPVHERVLRTCDDFVWPVDTTFLGRLIIDVEFLDLKIHPLNGGDASSIWPSDGKLHSVATQNTLRCLSRMLDKAIHVDLTIITADGTLKAHKAVMSATSPVFQASYHSSSEDKESSTIHIEDMSQESCTALLRYMYGTIKPEDFWKHRLALLGAANKYDIGDLKDACEESLLEDLNSGNVLERLNEAWLYQLHKLKKGCFAFLFDFGKIYDVRDEINTFFRHADRELMLEMFQEVLTIWK*