AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG001274

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG001274 MtrunA17_Chr1g0161431 94.268 628 26 2 1 620 1 626 0.0 1130
MsaG001274 MtrunA17_Chr4g0067981 57.099 324 129 5 10 329 28 345 4.14e-114 356
MsaG001274 MtrunA17_Chr2g0294911 59.416 308 116 3 10 315 26 326 1.02e-112 350
MsaG001274 MtrunA17_Chr8g0376881 57.778 270 107 3 13 281 25 288 2.82e-97 308
MsaG001274 MtrunA17_Chr3g0123131 57.353 272 109 2 12 281 12 278 6.26e-97 308
MsaG001274 MtrunA17_Chr3g0137101 54.362 298 114 5 9 299 4 286 9.04e-94 301
MsaG001274 MtrunA17_Chr1g0149841 53.462 260 116 2 12 270 4 259 6.83e-85 274
MsaG001274 MtrunA17_Chr4g0074411 48.288 292 140 5 13 304 18 298 5.60e-80 264
MsaG001274 MtrunA17_Chr2g0304841 49.338 302 139 5 13 304 18 315 1.21e-79 265
MsaG001274 MtrunA17_Chr4g0074401 47.727 308 143 7 2 304 6 300 6.54e-78 258
MsaG001274 MtrunA17_Chr7g0275891 46.844 301 119 8 12 288 4 287 8.01e-75 243
MsaG001274 MtrunA17_Chr1g0149851 47.122 278 109 3 12 264 4 268 4.84e-72 241
MsaG001274 MtrunA17_Chr7g0275401 43.434 297 138 3 12 294 4 284 7.81e-71 230
MsaG001274 MtrunA17_Chr4g0009851 46.371 248 102 4 11 258 9 225 1.13e-67 223
MsaG001274 MtrunA17_Chr4g0009901 47.773 247 98 4 13 259 10 225 1.23e-67 221
MsaG001274 MtrunA17_Chr4g0074421 56.410 195 75 5 63 256 2 187 5.41e-61 201
MsaG001274 MtrunA17_Chr6g0471041 43.145 248 108 5 12 258 13 228 6.74e-57 197
MsaG001274 MtrunA17_Chr1g0149831 50.500 200 91 3 72 270 1 193 3.60e-56 189
MsaG001274 MtrunA17_Chr4g0009981 44.279 201 82 1 58 258 2 172 7.28e-52 179
MsaG001274 MtrunA17_Chr3g0134461 45.082 244 78 3 9 251 4 192 1.08e-51 176
MsaG001274 MtrunA17_Chr3g0134481 58.156 141 54 1 10 150 5 140 3.50e-44 156
MsaG001274 MtrunA17_Chr3g0134481 61.111 54 21 0 200 253 130 183 2.21e-16 77.8
MsaG001274 MtrunA17_Chr8g0383961 48.026 152 77 2 11 162 12 161 1.88e-41 151
MsaG001274 MtrunA17_Chr8g0391911 35.827 254 128 5 13 251 9 242 4.72e-41 149
MsaG001274 MtrunA17_Chr4g0011071 37.059 170 77 1 59 228 35 174 1.87e-32 123
MsaG001274 MtrunA17_Chr6g0456631 33.333 165 107 2 12 174 8 171 1.13e-27 109
MsaG001274 MtrunA17_Chr6g0473731 35.751 193 94 5 93 284 10 173 1.26e-25 110
MsaG001274 MtrunA17_Chr8g0350761 29.921 254 163 4 15 254 19 271 1.91e-25 110
MsaG001274 MtrunA17_Chr4g0009991 42.177 147 54 3 105 251 1 116 3.02e-25 101
MsaG001274 MtrunA17_Chr3g0127941 43.089 123 65 2 17 135 56 177 7.20e-25 109
MsaG001274 MtrunA17_Chr5g0423031 53.086 81 37 1 13 93 10 89 5.30e-23 96.7
MsaG001274 MtrunA17_Chr7g0276361 36.585 164 86 4 14 162 11 171 2.08e-22 101
MsaG001274 MtrunA17_Chr4g0010001 51.852 81 38 1 13 93 10 89 3.19e-22 91.3
MsaG001274 MtrunA17_Chr4g0031961 58.442 77 28 1 188 260 113 189 1.76e-21 95.5
MsaG001274 MtrunA17_Chr8g0345901 41.322 121 66 2 19 135 38 157 3.62e-21 97.8
MsaG001274 MtrunA17_Chr4g0030271 34.161 161 91 3 17 163 89 248 5.99e-21 97.8
MsaG001274 MtrunA17_Chr3g0091641 31.220 205 125 5 17 205 24 228 6.43e-21 97.1
MsaG001274 MtrunA17_Chr1g0181811 33.537 164 94 3 17 166 85 247 7.59e-21 97.1
MsaG001274 MtrunA17_Chr7g0255711 64.516 62 22 0 199 260 88 149 1.99e-20 92.4
MsaG001274 MtrunA17_Chr4g0061021 28.796 191 122 3 17 200 33 216 1.60e-19 92.4
MsaG001274 MtrunA17_Chr4g0040481 37.778 135 72 2 138 260 82 216 2.84e-17 84.7
MsaG001274 MtrunA17_Chr5g0400241 60.000 60 24 0 201 260 310 369 4.60e-17 84.7
MsaG001274 MtrunA17_Chr5g0400241 36.434 129 76 3 12 138 13 137 3.41e-15 79.0
MsaG001274 MtrunA17_Chr4g0054981 60.000 60 24 0 201 260 309 368 1.83e-16 82.8
MsaG001274 MtrunA17_Chr4g0054981 34.375 128 80 2 12 138 13 137 3.56e-14 75.5
MsaG001274 MtrunA17_Chr2g0324891 56.061 66 29 0 195 260 154 219 6.76e-16 80.5
MsaG001274 MtrunA17_Chr5g0417521 57.627 59 24 1 198 255 11 69 5.31e-15 77.0
MsaG001274 MtrunA17_Chr6g0469321 64.151 53 19 0 218 270 70 122 1.06e-14 72.8
MsaG001274 MtrunA17_Chr6g0461641 46.753 77 32 2 198 273 38 106 3.67e-14 74.7
MsaG001274 MtrunA17_Chr4g0042451 54.237 59 26 1 198 255 13 71 9.03e-14 73.2
MsaG001274 MtrunA17_Chr2g0321461 54.237 59 26 1 198 255 41 99 1.14e-13 73.2
MsaG001274 MtrunA17_Chr2g0310011 59.322 59 23 1 198 255 31 89 1.34e-13 72.8
MsaG001274 MtrunA17_Chr8g0356501 59.322 59 23 1 198 255 33 91 1.61e-13 72.4
MsaG001274 MtrunA17_Chr1g0198601 51.471 68 32 1 195 261 204 271 1.86e-13 72.4
MsaG001274 MtrunA17_Chr7g0256551 29.054 148 83 3 17 142 9 156 1.02e-12 68.2
MsaG001274 MtrunA17_Chr7g0274471 62.963 54 19 1 202 254 218 271 1.44e-12 70.1
MsaG001274 MtrunA17_Chr5g0402631 48.649 74 37 1 199 271 211 284 1.49e-12 69.3
MsaG001274 MtrunA17_Chr5g0422621 47.143 70 36 1 201 269 197 266 2.70e-12 68.6
MsaG001274 MtrunA17_Chr4g0062921 48.052 77 37 2 194 269 227 301 3.43e-12 68.9
MsaG001274 MtrunA17_Chr7g0261911 52.381 63 29 1 198 259 258 320 4.76e-12 68.6
MsaG001274 MtrunA17_Chr4g0045361 52.381 63 29 1 199 260 271 333 5.51e-12 68.2
MsaG001274 MtrunA17_Chr1g0197171 58.182 55 22 1 202 255 44 98 5.68e-12 67.0
MsaG001274 MtrunA17_Chr1g0190301 52.941 68 25 2 194 254 161 228 7.80e-12 67.4
MsaG001274 MtrunA17_Chr7g0254981 54.545 55 24 1 202 255 50 104 2.03e-11 65.1
MsaG001274 MtrunA17_Chr2g0290991 48.649 74 33 2 183 255 24 93 4.10e-11 64.7
MsaG001274 MtrunA17_Chr1g0175081 47.458 59 30 1 198 255 18 76 6.81e-11 59.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG001274 AT3G16857.1 54.037 322 130 3 13 316 33 354 1.01e-106 337
MsaG001274 AT3G16857.2 54.037 322 130 3 13 316 33 354 1.71e-106 337
MsaG001274 AT2G01760.1 57.962 314 107 6 12 318 6 301 1.86e-106 327
MsaG001274 AT2G01760.2 53.216 342 107 7 12 318 6 329 2.85e-100 311
MsaG001274 AT3G16857.3 53.169 284 115 3 51 316 1 284 3.00e-89 290
MsaG001274 AT1G67710.1 59.608 255 93 5 10 260 4 252 5.92e-88 283
MsaG001274 AT2G25180.1 56.274 263 106 3 12 273 12 266 2.81e-87 283
MsaG001274 AT4G31920.1 52.344 256 103 2 12 267 12 248 9.89e-80 263
MsaG001274 AT2G01760.3 55.512 254 88 6 72 318 1 236 1.18e-74 242
MsaG001274 AT5G58080.1 46.154 312 140 5 12 311 13 308 1.57e-70 240
MsaG001274 AT1G67710.2 59.067 193 69 5 72 260 1 187 7.36e-60 207
MsaG001274 AT5G49240.1 38.868 265 141 6 10 267 35 285 1.81e-48 171
MsaG001274 AT1G49190.1 33.775 302 166 5 4 285 15 302 4.17e-44 162
MsaG001274 AT1G67710.3 54.335 173 69 5 92 260 12 178 5.73e-44 163
MsaG001274 AT3G62670.1 33.537 328 177 10 18 329 42 344 3.16e-40 152
MsaG001274 AT5G07210.2 31.111 270 157 6 17 261 16 281 1.15e-32 133
MsaG001274 AT5G07210.1 30.576 278 156 7 17 261 16 289 1.71e-30 127
MsaG001274 AT1G49190.2 42.143 140 75 1 4 137 15 154 3.91e-28 120
MsaG001274 AT2G27070.1 30.108 279 158 8 17 266 16 286 1.69e-25 111
MsaG001274 AT2G46790.3 39.516 124 70 2 17 136 37 159 5.70e-24 105
MsaG001274 AT2G46790.1 39.516 124 70 2 17 136 37 159 6.84e-24 105
MsaG001274 AT5G24470.1 40.323 124 69 2 16 135 49 171 4.69e-23 103
MsaG001274 AT5G60100.7 39.837 123 69 2 17 135 64 185 1.72e-22 100
MsaG001274 AT5G60100.6 39.837 123 69 2 17 135 64 185 1.72e-22 100
MsaG001274 AT5G60100.4 39.837 123 69 2 17 135 64 185 1.72e-22 100
MsaG001274 AT5G60100.5 39.516 124 70 2 17 136 64 186 3.05e-22 100
MsaG001274 AT5G60100.3 39.516 124 70 2 17 136 64 186 3.05e-22 100
MsaG001274 AT5G60100.1 39.516 124 70 2 17 136 64 186 3.05e-22 100
MsaG001274 AT5G59570.2 51.163 86 42 0 183 268 122 207 4.47e-22 97.4
MsaG001274 AT5G59570.1 51.163 86 42 0 183 268 122 207 4.47e-22 97.4
MsaG001274 AT5G60100.2 39.837 123 69 2 17 135 64 185 5.45e-22 100
MsaG001274 AT5G05090.1 62.687 67 24 1 195 260 73 139 3.24e-21 94.4
MsaG001274 AT3G46640.2 64.516 62 22 0 199 260 141 202 5.39e-21 94.7
MsaG001274 AT3G46640.1 64.516 62 22 0 199 260 141 202 5.39e-21 94.7
MsaG001274 AT3G46640.3 56.000 75 33 0 186 260 128 202 7.52e-21 94.4
MsaG001274 AT3G10760.1 62.687 67 24 1 195 260 97 163 7.96e-21 94.7
MsaG001274 AT2G40970.1 62.903 62 23 0 199 260 102 163 1.48e-20 92.0
MsaG001274 AT1G68210.1 25.556 270 175 7 17 261 13 281 4.94e-19 92.0
MsaG001274 AT5G44190.2 42.520 127 66 3 193 315 141 264 5.82e-19 89.4
MsaG001274 AT5G61380.1 35.246 122 76 1 17 135 19 140 6.42e-19 91.3
MsaG001274 AT5G44190.1 42.520 127 66 3 193 315 141 264 7.93e-19 89.4
MsaG001274 AT5G02810.1 37.398 123 72 2 17 135 78 199 2.33e-18 89.7
MsaG001274 AT2G20570.1 48.810 84 41 1 177 260 130 211 5.04e-16 81.3
MsaG001274 AT4G18020.3 34.586 133 78 1 137 260 222 354 5.45e-16 81.6
MsaG001274 AT4G18020.3 33.582 134 80 3 13 139 14 145 1.36e-12 70.9
MsaG001274 AT4G18020.2 34.586 133 78 1 137 260 222 354 5.45e-16 81.6
MsaG001274 AT4G18020.2 33.582 134 80 3 13 139 14 145 1.36e-12 70.9
MsaG001274 AT4G18020.1 34.586 133 78 1 137 260 222 354 5.45e-16 81.6
MsaG001274 AT4G18020.1 33.582 134 80 3 13 139 14 145 1.36e-12 70.9
MsaG001274 AT4G18020.6 34.586 133 78 1 137 260 222 354 5.45e-16 81.6
MsaG001274 AT4G18020.6 33.582 134 80 3 13 139 14 145 1.36e-12 70.9
MsaG001274 AT4G18020.9 34.586 133 78 1 137 260 222 354 5.45e-16 81.6
MsaG001274 AT4G18020.9 33.582 134 80 3 13 139 14 145 1.36e-12 70.9
MsaG001274 AT4G18020.8 34.586 133 78 1 137 260 222 354 5.84e-16 81.3
MsaG001274 AT4G18020.8 33.582 134 80 3 13 139 14 145 9.67e-13 71.2
MsaG001274 AT4G18020.11 34.586 133 78 1 137 260 222 354 5.84e-16 81.3
MsaG001274 AT4G18020.11 33.582 134 80 3 13 139 14 145 9.67e-13 71.2
MsaG001274 AT4G18020.4 34.586 133 78 1 137 260 222 354 5.84e-16 81.3
MsaG001274 AT4G18020.4 33.582 134 80 3 13 139 14 145 9.67e-13 71.2
MsaG001274 AT4G18020.5 34.586 133 78 1 137 260 222 354 5.84e-16 81.3
MsaG001274 AT4G18020.5 33.582 134 80 3 13 139 14 145 9.67e-13 71.2
MsaG001274 AT4G18020.10 34.586 133 78 1 137 260 222 354 5.84e-16 81.3
MsaG001274 AT4G18020.10 33.582 134 80 3 13 139 14 145 9.67e-13 71.2
MsaG001274 AT4G18020.7 34.586 133 78 1 137 260 222 354 5.84e-16 81.3
MsaG001274 AT4G18020.7 33.582 134 80 3 13 139 14 145 9.67e-13 71.2
MsaG001274 AT2G20570.2 64.912 57 20 0 204 260 171 227 1.87e-15 79.7
MsaG001274 AT3G04030.1 55.932 59 25 1 198 255 41 99 4.84e-14 74.7
MsaG001274 AT3G04030.3 55.932 59 25 1 198 255 41 99 5.21e-14 74.7
MsaG001274 AT5G18240.2 57.627 59 24 1 198 255 41 99 5.71e-14 74.7
MsaG001274 AT5G18240.3 57.627 59 24 1 198 255 41 99 5.71e-14 74.7
MsaG001274 AT5G18240.5 57.627 59 24 1 198 255 41 99 6.18e-14 74.7
MsaG001274 AT5G18240.1 57.627 59 24 1 198 255 41 99 6.19e-14 74.7
MsaG001274 AT5G18240.4 57.627 59 24 1 198 255 41 99 6.19e-14 74.7
MsaG001274 AT1G79430.2 59.322 59 23 1 198 255 30 88 2.06e-13 72.4
MsaG001274 AT3G24120.2 57.627 59 24 1 198 255 37 95 2.28e-13 71.6
MsaG001274 AT3G24120.1 57.627 59 24 1 198 255 37 95 2.33e-13 71.6
MsaG001274 AT4G13640.2 57.627 59 24 1 198 255 33 91 5.30e-13 70.5
MsaG001274 AT4G13640.1 57.627 59 24 1 198 255 33 91 5.32e-13 70.5
MsaG001274 AT3G04030.2 51.724 58 28 0 198 255 41 98 5.97e-13 71.6
MsaG001274 AT1G69580.2 52.542 59 27 1 198 255 30 88 6.90e-13 70.9
MsaG001274 AT1G69580.1 52.542 59 27 1 198 255 30 88 7.22e-13 70.5
MsaG001274 AT3G12730.1 54.237 59 26 1 198 255 19 77 2.82e-12 67.4
MsaG001274 AT3G57040.2 28.276 145 82 2 19 141 11 155 1.26e-11 65.5
MsaG001274 AT3G57040.1 28.276 145 82 2 19 141 11 155 1.26e-11 65.5
MsaG001274 AT5G45580.1 52.727 55 25 1 202 255 7 61 1.91e-11 65.5
MsaG001274 AT2G01060.1 50.725 69 31 2 190 255 1 69 2.02e-11 65.5
MsaG001274 AT3G04450.3 51.724 58 27 1 198 254 234 291 4.35e-11 65.1
MsaG001274 AT5G29000.4 55.172 58 24 2 198 254 228 284 6.26e-11 64.3
MsaG001274 AT3G04450.4 51.724 58 27 1 198 254 194 251 6.60e-11 65.1
MsaG001274 AT3G04450.2 51.724 58 27 1 198 254 194 251 6.60e-11 65.1
MsaG001274 AT3G04450.1 51.724 58 27 1 198 254 234 291 6.67e-11 65.5
MsaG001274 AT5G29000.1 55.172 58 24 2 198 254 185 241 9.88e-11 64.3
MsaG001274 AT5G29000.3 55.172 58 24 2 198 254 185 241 9.88e-11 64.3

Find 136 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTGCTGAATATTGTTATTTA+TGG 0.028003 1:-17657739 None:intergenic
TGAAGAGCTGCAAGTGTTTC+AGG 0.164593 1:-17657144 None:intergenic
AACGAGCTCCGGTGAGTTTC+CGG 0.202869 1:+17651774 MsaT001274.1:CDS
GTGAGTTTCCGGCTGGGTTA+AGG 0.272505 1:+17651785 MsaT001274.1:CDS
CTTAGAAATCTTGGATTTGT+TGG 0.296022 1:+17657879 MsaT001274.1:CDS
GCTCTTCAGGCGGAGCTCTT+AGG 0.299128 1:+17657159 MsaT001274.1:CDS
GTCAACAAGGATAGCAGTTT+TGG 0.309228 1:+17657585 MsaT001274.1:CDS
AATAAGGAACATGATAATTC+TGG 0.316091 1:+17655302 MsaT001274.1:CDS
TGAAACACTTGCAGCTCTTC+AGG 0.318742 1:+17657146 MsaT001274.1:CDS
TAAAATCATTTGCAGATGAT+TGG 0.319690 1:-17657938 None:intergenic
TGAGTTTCCGGCTGGGTTAA+GGG 0.325512 1:+17651786 MsaT001274.1:CDS
TAGTCAAATGAAAGTATTCA+TGG 0.326858 1:+17657959 MsaT001274.1:CDS
ATATGACTCGGTTCCTCCTT+TGG 0.330245 1:-17658011 None:intergenic
GAAGTATGTCTTAGAAATCT+TGG 0.340115 1:+17657870 MsaT001274.1:CDS
CAAAACTCAACTTTAACATT+TGG 0.341966 1:+17657765 MsaT001274.1:CDS
GAATCCAAGCTTGCTGCTAC+TGG 0.348004 1:+17657078 MsaT001274.1:CDS
ATCATTTCGAGTATTCTCTT+TGG 0.348591 1:-17655707 None:intergenic
ATACAGGCTTTGGCTGGTTC+TGG 0.349018 1:+17657111 MsaT001274.1:CDS
AAATGTTGCAAGTCATTTGC+AGG 0.369685 1:+17655754 MsaT001274.1:CDS
GATGAGTCATCATCAGTATA+TGG 0.370458 1:+17657330 MsaT001274.1:CDS
AGAGGATATTTGAGGAGAAA+TGG 0.372882 1:-17657703 None:intergenic
TGGAAGAACTAAGGAATATA+TGG 0.378920 1:+17655249 MsaT001274.1:CDS
GTAGGCATTATGTTAGTTGC+CGG 0.379535 1:-17657186 None:intergenic
GACAACACTTCTTCTGTTGC+TGG 0.380394 1:+17655371 MsaT001274.1:CDS
CTTTGAAAATTCTGCGAGAA+AGG 0.380677 1:+17654946 MsaT001274.1:CDS
GACCAGTTCTTACTAATAAC+AGG 0.381518 1:-17655062 None:intergenic
TCAATCTTCTAATACCTTCC+AGG 0.399165 1:+17657542 MsaT001274.1:CDS
GATGGATAATTCTGTAGAAC+TGG 0.405841 1:-17658056 None:intergenic
TCGCTGGATTTAATATCATA+TGG 0.407074 1:-17657837 None:intergenic
CGAGGCTTCTTAGATGTAGA+TGG 0.408023 1:-17655473 None:intergenic
AGAACTGGGTGGCTCGCATT+TGG 0.408581 1:-17658041 None:intergenic
AATCTTGGATTTGTTGGAAA+AGG 0.408723 1:+17657885 MsaT001274.1:CDS
GCTGATGCTGGTGCCGTTGT+TGG 0.410400 1:-17657443 None:intergenic
TCTGTTGCTGGTGCAACAAA+AGG 0.420915 1:+17655383 MsaT001274.1:CDS
AGCTCCGGTGAGTTTCCGGC+TGG 0.423241 1:+17651778 MsaT001274.1:CDS
TGAACATGTTGGGTTGGAAA+TGG 0.424268 1:+17655034 MsaT001274.1:CDS
GGGATGTTAAACACAGTTCA+TGG 0.434178 1:+17657048 MsaT001274.1:CDS
ATAAACTCCTTGAACATGTT+GGG 0.438608 1:+17655024 MsaT001274.1:CDS
AGTTTATAGCCATCCATGTC+TGG 0.448144 1:-17655008 None:intergenic
TTTCCAACCCAACATGTTCA+AGG 0.451146 1:-17655031 None:intergenic
TGTTCAACCATCTTGCTTGG+TGG 0.455635 1:+17657512 MsaT001274.1:CDS
TGATTGACTATAATCAATAA+TGG 0.457615 1:-17657616 None:intergenic
AAGATATGACATACAGGCTT+TGG 0.459864 1:+17657101 MsaT001274.1:CDS
AAAAGATGAAGATGTTGAGT+TGG 0.460862 1:+17655439 MsaT001274.1:CDS
TCTTCCAGTAGCAGCAAGCT+TGG 0.461683 1:-17657082 None:intergenic
CTTAGTTCTTCCATCCGTAC+AGG 0.475894 1:-17655239 None:intergenic
TCTCTTCGCTGTCATTATCA+AGG 0.476993 1:+17651859 MsaT001274.1:CDS
ACTAATGCATGCATCTGTAC+AGG 0.484480 1:+17657224 MsaT001274.1:CDS
CAATGTTCAACCATCTTGCT+TGG 0.486283 1:+17657509 MsaT001274.1:CDS
TACTTCATCAAGTGAATCGC+TGG 0.487337 1:-17657853 None:intergenic
GGATATTTGAGGAGAAATGG+AGG 0.494211 1:-17657700 None:intergenic
TATAAACTCCTTGAACATGT+TGG 0.495307 1:+17655023 MsaT001274.1:CDS
CAGTGATGTCTGTTGATGAT+AGG 0.497216 1:+17655162 MsaT001274.1:intron
GAAGAGCTGCAAGTGTTTCA+GGG 0.504164 1:-17657143 None:intergenic
CACAGTGAAGGAGGAGCCAA+AGG 0.506659 1:+17657995 MsaT001274.1:CDS
TATGACATACAGGCTTTGGC+TGG 0.508995 1:+17657105 MsaT001274.1:CDS
GTAGCTCTTGTGTCTCCGCC+AGG 0.509868 1:-17657678 None:intergenic
GTCTAAGTGTCAAGGGGAGA+AGG 0.513330 1:-17657809 None:intergenic
AATTTAGACTTTATTTGAAG+AGG 0.514433 1:+17656998 MsaT001274.1:CDS
TCGAGTTCATGTCAATATCC+TGG 0.525235 1:+17657660 MsaT001274.1:CDS
CTCGTTCTCTGCAACGACGC+AGG 0.525983 1:-17651757 None:intergenic
ACTCCTTGAACATGTTGGGT+TGG 0.526711 1:+17655028 MsaT001274.1:CDS
AGTGTCAAGGGGAGAAGGCT+AGG 0.528820 1:-17657804 None:intergenic
TTCGAGTATTCTCTTTGGCA+CGG 0.530409 1:-17655702 None:intergenic
GTGGTGGCGCATCAACAGAA+TGG 0.530597 1:+17657027 MsaT001274.1:CDS
TACTGGAAGATATGACATAC+AGG 0.531861 1:+17657095 MsaT001274.1:CDS
TAGGCATTATGTTAGTTGCC+GGG 0.533217 1:-17657185 None:intergenic
CTAATGCATGCATCTGTACA+GGG 0.539995 1:+17657225 MsaT001274.1:CDS
AAGCAACACTATTTACTGCC+TGG 0.549477 1:-17657560 None:intergenic
TGGCCGCCGTCTAGTACACT+CGG 0.552222 1:+17657357 MsaT001274.1:CDS
GGCGGAGCTCTTAGGTCACC+CGG 0.553138 1:+17657167 MsaT001274.1:CDS
TAGGACAAGTACTGTCATGA+AGG 0.556092 1:+17655181 MsaT001274.1:CDS
AACACTATTTACTGCCTGGA+AGG 0.557646 1:-17657556 None:intergenic
CTCGAAATGATGAATGTCCC+TGG 0.557903 1:+17655719 MsaT001274.1:CDS
GGAACTTGTCTAAGTGTCAA+GGG 0.557917 1:-17657816 None:intergenic
TTTGAAGAGGTTAAGTGTGG+TGG 0.558644 1:+17657011 MsaT001274.1:CDS
TCAATCTCAAGTGGAAGGTT+AGG 0.559134 1:+17657381 MsaT001274.1:CDS
TCGTTGCAGAGAACGAGCTC+CGG 0.559573 1:+17651763 MsaT001274.1:CDS
TAACCCAGCCGGAAACTCAC+CGG 0.561846 1:-17651782 None:intergenic
AAATTATTGACGATGTCGAA+AGG 0.566946 1:-17657285 None:intergenic
TGGAACTTGTCTAAGTGTCA+AGG 0.569185 1:-17657817 None:intergenic
CTGTACGGATGGAAGAACTA+AGG 0.571403 1:+17655240 MsaT001274.1:CDS
AGATAATGATCGCAATACAC+GGG 0.572667 1:+17655334 MsaT001274.1:CDS
ATGCATGCATTAGTGCAGTC+CGG 0.579695 1:-17657215 None:intergenic
AATTATTGACGATGTCGAAA+GGG 0.582045 1:-17657284 None:intergenic
ATATATGGCAGCATGTTGCT+AGG 0.583349 1:+17655264 MsaT001274.1:CDS
AAAATTCTGCGAGAAAGGAA+AGG 0.584113 1:+17654951 MsaT001274.1:CDS
TGGTGGCGCATCAACAGAAT+GGG 0.586758 1:+17657028 MsaT001274.1:CDS
AGTGCAGTCCGGTCCACTGT+AGG 0.587668 1:-17657204 None:intergenic
ACATAATGCCTACAGTGGAC+CGG 0.591447 1:+17657196 MsaT001274.1:CDS
TTATTTGAAGAGGTTAAGTG+TGG 0.592282 1:+17657008 MsaT001274.1:CDS
AAGATAATGATCGCAATACA+CGG 0.600067 1:+17655333 MsaT001274.1:CDS
AAAGTATTCATGGCTAGCAA+TGG 0.600983 1:+17657969 MsaT001274.1:CDS
ACAAGAACCCTTAACCCAGC+CGG 0.601519 1:-17651793 None:intergenic
CAATGGTAACACAGTGAAGG+AGG 0.603246 1:+17657986 MsaT001274.1:CDS
GTCAGTGCTGTAAATCAACT+AGG 0.604605 1:+17655530 MsaT001274.1:CDS
AAGGTATTAGAAGATTGAGC+AGG 0.607200 1:-17657537 None:intergenic
TCATGAAGGGAATTAGACAC+GGG 0.611444 1:+17655195 MsaT001274.1:CDS
TAGTGTTGCTTCTGTCAACA+AGG 0.611545 1:+17657572 MsaT001274.1:CDS
GGATGTTAAACACAGTTCAT+GGG 0.616102 1:+17657049 MsaT001274.1:CDS
GCTCCGGTGAGTTTCCGGCT+GGG 0.616703 1:+17651779 MsaT001274.1:CDS
TTTGGATATCTGATATGACT+CGG 0.618478 1:-17658023 None:intergenic
CATCATCAGTATATGGAGCA+TGG 0.620082 1:+17657337 MsaT001274.1:CDS
AACACTTGCAGCTCTTCAGG+CGG 0.620247 1:+17657149 MsaT001274.1:CDS
GCAGGAACCACCAAGCAAGA+TGG 0.620808 1:-17657519 None:intergenic
AGGACAAGTACTGTCATGAA+GGG 0.622788 1:+17655182 MsaT001274.1:CDS
GACGATGTCGAAAGGGCTGT+AGG 0.623972 1:-17657277 None:intergenic
GCACTTGAATGATCATGAGA+TGG 0.624506 1:-17658074 None:intergenic
GTCATGAAGGGAATTAGACA+CGG 0.627814 1:+17655194 MsaT001274.1:CDS
CAAGTGTTTCAGGGGAAACG+TGG 0.629851 1:-17657134 None:intergenic
CTAAGAAGCCTCGTGTAGTG+TGG 0.636384 1:+17655483 MsaT001274.1:CDS
AAGGGGAGAAGGCTAGGCAA+TGG 0.637218 1:-17657798 None:intergenic
TCGGGTCAATCTCAAGTGGA+AGG 0.637487 1:+17657376 MsaT001274.1:CDS
AAGAGCTGCAAGTGTTTCAG+GGG 0.639399 1:-17657142 None:intergenic
TAGCAATGGTAACACAGTGA+AGG 0.641107 1:+17657983 MsaT001274.1:CDS
AGTGAAGGAGGAGCCAAAGG+AGG 0.646617 1:+17657998 MsaT001274.1:CDS
GTTCATATGCCAGACATGGA+TGG 0.651624 1:+17654999 MsaT001274.1:CDS
ACACTCGGGTCAATCTCAAG+TGG 0.652626 1:+17657372 MsaT001274.1:CDS
ATATATTGCAGCACCAACAA+CGG 0.653116 1:+17657430 MsaT001274.1:CDS
GAACTTGTCTAAGTGTCAAG+GGG 0.665309 1:-17657815 None:intergenic
GATAATGATCGCAATACACG+GGG 0.671509 1:+17655335 MsaT001274.1:CDS
GGCCGCCGTCTAGTACACTC+GGG 0.673948 1:+17657358 MsaT001274.1:CDS
TGATGTTCATATGCCAGACA+TGG 0.674601 1:+17654995 MsaT001274.1:CDS
CGTGCAGAATAGTAACACAC+TGG 0.679139 1:+17657404 MsaT001274.1:CDS
AGTTCTTACTAATAACAGGA+AGG 0.681007 1:-17655058 None:intergenic
TGCAGTCACTACATGCTCTG+AGG 0.686470 1:+17654914 MsaT001274.1:intron
TCTACTGACCACACTACACG+AGG 0.699174 1:-17655491 None:intergenic
GATAATTCTGTAGAACTGGG+TGG 0.703628 1:-17658052 None:intergenic
AACTAACATAATGCCTACAG+TGG 0.706212 1:+17657191 MsaT001274.1:CDS
TAATGCATGCATCTGTACAG+GGG 0.713759 1:+17657226 MsaT001274.1:CDS
ATGGATAATTCTGTAGAACT+GGG 0.722325 1:-17658055 None:intergenic
CATGAAGGGAATTAGACACG+GGG 0.726759 1:+17655196 MsaT001274.1:CDS
AGTTCATGTCAATATCCTGG+CGG 0.734679 1:+17657663 MsaT001274.1:CDS
GACCCGAGTGTACTAGACGG+CGG 0.735269 1:-17657360 None:intergenic
ATTGACCCGAGTGTACTAGA+CGG 0.751718 1:-17657363 None:intergenic
ATAATGATCGCAATACACGG+GGG 0.815289 1:+17655336 MsaT001274.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr1 gene 17651754 17658106 17651754 ID=MsaG001274
Chr1 mRNA 17651754 17658106 17651754 ID=MsaT001274.1;Parent=MsaG001274
Chr1 exon 17651754 17651880 17651754 ID=MsaT001274.1.exon1;Parent=MsaT001274.1
Chr1 CDS 17651754 17651880 17651754 ID=cds.MsaT001274.1;Parent=MsaT001274.1
Chr1 exon 17654919 17655071 17654919 ID=MsaT001274.1.exon2;Parent=MsaT001274.1
Chr1 CDS 17654919 17655071 17654919 ID=cds.MsaT001274.1;Parent=MsaT001274.1
Chr1 exon 17655165 17655560 17655165 ID=MsaT001274.1.exon3;Parent=MsaT001274.1
Chr1 CDS 17655165 17655560 17655165 ID=cds.MsaT001274.1;Parent=MsaT001274.1
Chr1 exon 17655699 17655775 17655699 ID=MsaT001274.1.exon4;Parent=MsaT001274.1
Chr1 CDS 17655699 17655775 17655699 ID=cds.MsaT001274.1;Parent=MsaT001274.1
Chr1 exon 17656997 17658106 17656997 ID=MsaT001274.1.exon5;Parent=MsaT001274.1
Chr1 CDS 17656997 17658106 17656997 ID=cds.MsaT001274.1;Parent=MsaT001274.1
Gene Sequence

>MsaT001274.1

ATGCCTGCGTCGTTGCAGAGAACGAGCTCCGGTGAGTTTCCGGCTGGGTTAAGGGTTCTTGTTGTTGATGATGACGTCACTACGTTGAAGATAATTGAACAAATGTCTCTTCGCTGTCATTATCAAGTCACTACATGCTCTGAGGCTACTGTTGCTTTGAAAATTCTGCGAGAAAGGAAAGGCTGTTTTGATGTTGTGCTGAGTGATGTTCATATGCCAGACATGGATGGCTATAAACTCCTTGAACATGTTGGGTTGGAAATGGACCTTCCTGTTATTATGATGTCTGTTGATGATAGGACAAGTACTGTCATGAAGGGAATTAGACACGGGGCTTGTGATTATTTTATTAAGCCTGTACGGATGGAAGAACTAAGGAATATATGGCAGCATGTTGCTAGGAAAATTTTGAATGAAAATAAGGAACATGATAATTCTGGCAGCATAGAAGATAATGATCGCAATACACGGGGGAATGATGATAATGACAACACTTCTTCTGTTGCTGGTGCAACAAAAGGAATAGTAAAAGAACAAAAAAAGAGGAGCAATTTAAAAGATGAAGATGTTGAGTTGGAGAATGACGATCCATCTACATCTAAGAAGCCTCGTGTAGTGTGGTCAGTAGAACTGCATCAACAATTTGTCAGTGCTGTAAATCAACTAGGACTTGACAAGGCCGTGCCAAAGAGAATACTCGAAATGATGAATGTCCCTGGTCTAACAAGAGAAAATGTTGCAAGTCATTTGCAGAAATTTAGACTTTATTTGAAGAGGTTAAGTGTGGTGGCGCATCAACAGAATGGGATGTTAAACACAGTTCATGGGACTATAGAATCCAAGCTTGCTGCTACTGGAAGATATGACATACAGGCTTTGGCTGGTTCTGGCCACGTTTCCCCTGAAACACTTGCAGCTCTTCAGGCGGAGCTCTTAGGTCACCCGGCAACTAACATAATGCCTACAGTGGACCGGACTGCACTAATGCATGCATCTGTACAGGGGACAAAACATTCTCGTGCTGATGATCATGCCTACAGCCCTTTCGACATCGTCAATAATTTCCCTCAGTCCATCTTAAAAGTAGATGAGTCATCATCAGTATATGGAGCATGGCCGCCGTCTAGTACACTCGGGTCAATCTCAAGTGGAAGGTTAGGCGTGCAGAATAGTAACACACTGGTTGATATATTGCAGCACCAACAACGGCACCAGCATCAGCAAAAACAACAGCAGTCTCAGATGCATGATCAAAATCGATCAATCAATGTTCAACCATCTTGCTTGGTGGTTCCTGCTCAATCTTCTAATACCTTCCAGGCAGTAAATAGTGTTGCTTCTGTCAACAAGGATAGCAGTTTTGGCAGAAATGCCATTATTGATTATAGTCAATCATCACAAAAATCAAATACTTCATCGAGTTCATGTCAATATCCTGGCGGAGACACAAGAGCTACCTCCATTTCTCCTCAAATATCCTCTTTTTCAGTAGGATCCATAAATAACAATATTCAGCAGATTCAAAACTCAACTTTAACATTTGGTGCTGCTAGACCATTGCCTAGCCTTCTCCCCTTGACACTTAGACAAGTTCCATATGATATTAAATCCAGCGATTCACTTGATGAAGTATGTCTTAGAAATCTTGGATTTGTTGGAAAAGGTACCTGCATACCAAGTTTTGAAAATAAAATCCAATCATCTGCAAATGATTTTAGTCAAATGAAAGTATTCATGGCTAGCAATGGTAACACAGTGAAGGAGGAGCCAAAGGAGGAACCGAGTCATATCAGATATCCAAATGCGAGCCACCCAGTTCTACAGAATTATCCATCTCATGATCATTCAAGTGCATTCACTTAA
Protein sequence

>MsaT001274.1

MPASLQRTSSGEFPAGLRVLVVDDDVTTLKIIEQMSLRCHYQVTTCSEATVALKILRERKGCFDVVLSDVHMPDMDGYKLLEHVGLEMDLPVIMMSVDDRTSTVMKGIRHGACDYFIKPVRMEELRNIWQHVARKILNENKEHDNSGSIEDNDRNTRGNDDNDNTSSVAGATKGIVKEQKKRSNLKDEDVELENDDPSTSKKPRVVWSVELHQQFVSAVNQLGLDKAVPKRILEMMNVPGLTRENVASHLQKFRLYLKRLSVVAHQQNGMLNTVHGTIESKLAATGRYDIQALAGSGHVSPETLAALQAELLGHPATNIMPTVDRTALMHASVQGTKHSRADDHAYSPFDIVNNFPQSILKVDESSSVYGAWPPSSTLGSISSGRLGVQNSNTLVDILQHQQRHQHQQKQQQSQMHDQNRSINVQPSCLVVPAQSSNTFQAVNSVASVNKDSSFGRNAIIDYSQSSQKSNTSSSSCQYPGGDTRATSISPQISSFSVGSINNNIQQIQNSTLTFGAARPLPSLLPLTLRQVPYDIKSSDSLDEVCLRNLGFVGKGTCIPSFENKIQSSANDFSQMKVFMASNGNTVKEEPKEEPSHIRYPNASHPVLQNYPSHDHSSAFT*