AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG001278


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG001278 MtrunA17_Chr1g0161461 90.691 333 20 5 1 328 29 355 0.0 590
MsaG001278 MtrunA17_Chr4g0038511 57.676 241 85 5 51 284 6 236 1.36e-79 244
MsaG001278 MtrunA17_Chr4g0060591 43.137 102 58 0 50 151 27 128 1.27e-22 94.4
MsaG001278 MtrunA17_Chr4g0042041 37.838 148 80 4 5 148 35 174 7.55e-20 87.0
MsaG001278 MtrunA17_Chr2g0319301 44.000 100 51 2 56 154 74 169 1.34e-19 86.3
MsaG001278 MtrunA17_Chr2g0323031 44.565 92 50 1 56 146 110 201 9.05e-18 81.6
MsaG001278 MtrunA17_Chr1g0167821 37.423 163 76 6 56 217 95 232 2.60e-17 80.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG001278 AT3G19860.1 51.639 244 109 4 49 290 3 239 1.70e-75 234
MsaG001278 AT3G19860.3 50.996 251 114 4 42 290 27 270 5.41e-75 233
MsaG001278 AT3G19860.2 50.996 251 114 4 42 290 49 292 2.00e-74 233
MsaG001278 AT4G36060.2 40.486 247 95 8 55 289 30 236 1.13e-47 162
MsaG001278 AT4G36060.1 40.486 247 95 8 55 289 48 254 4.35e-47 160
MsaG001278 AT4G36060.4 40.506 237 90 7 55 280 30 226 1.38e-45 156
MsaG001278 AT4G36060.3 40.506 237 90 7 55 280 48 244 4.94e-45 155
MsaG001278 AT5G54680.1 43.000 100 52 2 56 154 76 171 6.50e-19 84.7
MsaG001278 AT3G23210.2 39.130 138 73 5 56 182 138 275 1.10e-18 85.1
MsaG001278 AT3G23210.1 39.130 138 73 5 56 182 167 304 2.03e-18 84.7
MsaG001278 AT1G51070.1 45.745 94 50 1 56 148 71 164 4.76e-17 79.3
MsaG001278 AT1G51070.2 45.745 94 50 1 56 148 137 230 1.15e-16 79.3
MsaG001278 AT3G47640.1 33.645 107 71 0 50 156 26 132 2.61e-16 77.4
MsaG001278 AT3G47640.3 33.645 107 71 0 50 156 26 132 2.61e-16 77.4
MsaG001278 AT3G47640.2 33.645 107 71 0 50 156 26 132 2.61e-16 77.4
MsaG001278 AT4G14410.2 34.028 144 73 4 56 198 129 251 3.37e-14 72.0
MsaG001278 AT4G14410.3 34.028 144 73 4 56 198 129 251 3.37e-14 72.0
MsaG001278 AT4G14410.1 34.028 144 73 4 56 198 135 257 4.44e-14 72.0

Find 83 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGGAATATGACCAGGATTT+TGG 0.212723 1:-17720964 None:intergenic
CATCAATTGGGAAACGTAAT+AGG 0.223751 1:+17719738 MsaT001278.1:CDS
ATAGGAACGGGGTACGTATA+AGG 0.292057 1:-17720872 None:intergenic
TTAAAGACGCTTTCTCTTCT+CGG 0.308272 1:-17720754 None:intergenic
GTGTTTAGCGGCGGAGGCGG+TGG 0.314738 1:-17719463 None:intergenic
TTGTCATTCCGTGGTCTATC+AGG 0.332142 1:-17720522 None:intergenic
CTCAATGTATATTCCATTCT+CGG 0.354937 1:+17720994 MsaT001278.1:CDS
TCAAGCTCTGTTGCCACATC+TGG 0.367803 1:-17721154 None:intergenic
TGAGCTGATGGCATCTCTAT+TGG 0.379396 1:-17721031 None:intergenic
AGGCTGACCGTGAGAAAATC+AGG 0.380020 1:+17719691 MsaT001278.1:CDS
GAGCCAGTATTCTGCATCTC+AGG 0.388043 1:+17721369 MsaT001278.1:CDS
AAAGTCAATTAACTAGCCTT+TGG 0.391631 1:-17721427 None:intergenic
ATCTCTATTGGAGGGATAGC+TGG 0.399284 1:-17721019 None:intergenic
ATTGGCATTGGCATAGGAAC+GGG 0.403675 1:-17720884 None:intergenic
GTCCCTGAGATGCAGAATAC+TGG 0.415172 1:-17721372 None:intergenic
TGTTTAGCGGCGGAGGCGGT+GGG 0.428409 1:-17719462 None:intergenic
TTGAATTGTCTCTGTGATGA+TGG 0.441426 1:-17720550 None:intergenic
TAGGAACGGGGTACGTATAA+GGG 0.451220 1:-17720871 None:intergenic
TGGAGTGTTGCTGACGCTGT+TGG 0.452978 1:-17721407 None:intergenic
TTCGAGGTGTTTAGCGGCGG+AGG 0.460101 1:-17719469 None:intergenic
TGGTTTCCATAGTAAGGAAA+AGG 0.470758 1:-17720944 None:intergenic
GAGCTTGAGCTTAAGATGCC+TGG 0.473388 1:+17721169 MsaT001278.1:CDS
GCCTGGATCATCAACACAAC+AGG 0.473485 1:+17721186 MsaT001278.1:CDS
GGGTGAATGGAAACCGGACC+AGG 0.474052 1:-17720911 None:intergenic
AATGCCAATCCCTCCTGGTC+CGG 0.475088 1:+17720898 MsaT001278.1:CDS
GCATGGCTCTGACTCTCTGC+TGG 0.479835 1:-17720808 None:intergenic
GTGATTCTTCTGCCGCAGTA+AGG 0.481448 1:-17719501 None:intergenic
AGGTTGCATAGGTGGGTGAA+TGG 0.481673 1:-17720924 None:intergenic
GAAACCGGACCAGGAGGGAT+TGG 0.482079 1:-17720902 None:intergenic
AATATCTTTACGATTGGTGA+TGG 0.482957 1:-17719533 None:intergenic
GAGGTGTTTAGCGGCGGAGG+CGG 0.484202 1:-17719466 None:intergenic
CTAAACACGTGGGATGTAGA+AGG 0.488709 1:-17721058 None:intergenic
CCTGACAATATCTTTACGAT+TGG 0.490047 1:-17719539 None:intergenic
ATGCCAATGCCAATCCCTCC+TGG 0.495420 1:+17720893 MsaT001278.1:CDS
GGGATAGCTGGAGCCGAGAA+TGG 0.495557 1:-17721007 None:intergenic
TTACGATTGGTGATGGGCAG+CGG 0.497262 1:-17719526 None:intergenic
TACTATGGAAACCAAAATCC+TGG 0.503568 1:+17720953 MsaT001278.1:CDS
TAAGGAAAAGGTTGCATAGG+TGG 0.507917 1:-17720932 None:intergenic
GTGTAGAGAGAAGAGTGAGA+TGG 0.512423 1:+17719635 MsaT001278.1:intron
AGAGACAATTCAAGTGCTAA+AGG 0.518046 1:+17720560 MsaT001278.1:CDS
TTTCTAGCAACAACAACACT+TGG 0.522874 1:-17719660 None:intergenic
CCAATCGTAAAGATATTGTC+AGG 0.525025 1:+17719539 MsaT001278.1:CDS
TGCAACACATGATCAATCAA+TGG 0.526037 1:+17719437 None:intergenic
AATTGCAGTCCATGGTGGCA+TGG 0.531488 1:-17720825 None:intergenic
GCTGATGGCATCTCTATTGG+AGG 0.536435 1:-17721028 None:intergenic
CTATAGGATTGCACATCTAG+AGG 0.536684 1:+17721289 MsaT001278.1:intron
CTACATCTTTCTGCATCACT+AGG 0.537613 1:-17721124 None:intergenic
ACCTGTTGTGTTGATGATCC+AGG 0.545050 1:-17721187 None:intergenic
GGAGGGATTGGCATTGGCAT+AGG 0.546018 1:-17720890 None:intergenic
GAATGGTCAATTGCAGTCCA+TGG 0.547989 1:-17720833 None:intergenic
ATAGGTGGGTGAATGGAAAC+CGG 0.548801 1:-17720917 None:intergenic
CTAGGTCTTCTTCTATGACC+TGG 0.556746 1:-17721106 None:intergenic
GGACCAGGAGGGATTGGCAT+TGG 0.561637 1:-17720896 None:intergenic
TAGTAAGGAAAAGGTTGCAT+AGG 0.564004 1:-17720935 None:intergenic
TGATTCTTCTGCCGCAGTAA+GGG 0.566675 1:-17719500 None:intergenic
AAGGAAAAGGTTGCATAGGT+GGG 0.567952 1:-17720931 None:intergenic
GATTGGCATTGGCATAGGAA+CGG 0.568920 1:-17720885 None:intergenic
GTAGAAGGATACTGAGCTGA+TGG 0.572163 1:-17721043 None:intergenic
ATATCTTTACGATTGGTGAT+GGG 0.578157 1:-17719532 None:intergenic
TGTTGCTAGAAAAGTTCAGA+AGG 0.582816 1:+17719671 MsaT001278.1:CDS
AGCCAGTATTCTGCATCTCA+GGG 0.590410 1:+17721370 MsaT001278.1:CDS
ATATACATTGAGGATAGACT+TGG 0.594802 1:-17720983 None:intergenic
TCTTCTGCCGCAGTAAGGGA+TGG 0.602962 1:-17719496 None:intergenic
GAGTCTCGAAGCAAGTCACC+AGG 0.602988 1:+17721088 MsaT001278.1:CDS
AGAGCATAAATCACTTTCTG+AGG 0.607090 1:+17720614 MsaT001278.1:CDS
CGAGAATGGAATATACATTG+AGG 0.609687 1:-17720993 None:intergenic
GATAGACTTGGAATATGACC+AGG 0.610908 1:-17720971 None:intergenic
CGATTGGTGATGGGCAGCGG+AGG 0.614010 1:-17719523 None:intergenic
ATGGTTGCCTTGTCATTCCG+TGG 0.623588 1:-17720531 None:intergenic
TGAATGGAAACCGGACCAGG+AGG 0.624977 1:-17720908 None:intergenic
GGCTACTCATGACAACAGAA+TGG 0.634259 1:-17720850 None:intergenic
GAATGGAAACCGGACCAGGA+GGG 0.634490 1:-17720907 None:intergenic
CTGATGGCATCTCTATTGGA+GGG 0.634505 1:-17721027 None:intergenic
CAGCGTCAGCAACACTCCAA+AGG 0.635368 1:+17721411 MsaT001278.1:CDS
GAGTCAGAGCCATGCCACCA+TGG 0.644309 1:+17720816 MsaT001278.1:CDS
TGATAGACCACGGAATGACA+AGG 0.652628 1:+17720524 MsaT001278.1:CDS
TTGGCATTGGCATAGGAACG+GGG 0.655443 1:-17720883 None:intergenic
ATGTAGTGTGTCTCCAGATG+TGG 0.671060 1:+17721141 MsaT001278.1:CDS
ATGCAGATCCTGATAGACCA+CGG 0.688242 1:+17720514 MsaT001278.1:intron
TGGTCAATTGCAGTCCATGG+TGG 0.700069 1:-17720830 None:intergenic
TGACCGTGAGAAAATCAGGA+GGG 0.711741 1:+17719695 MsaT001278.1:CDS
CTGACCGTGAGAAAATCAGG+AGG 0.717963 1:+17719694 MsaT001278.1:CDS
GAAAACACCATCCCTTACTG+CGG 0.761554 1:+17719489 MsaT001278.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr1 gene 17719445 17721441 17719445 ID=MsaG001278
Chr1 mRNA 17719445 17721441 17719445 ID=MsaT001278.1;Parent=MsaG001278
Chr1 exon 17719445 17719560 17719445 ID=MsaT001278.1.exon1;Parent=MsaT001278.1
Chr1 CDS 17719445 17719560 17719445 ID=cds.MsaT001278.1;Parent=MsaT001278.1
Chr1 exon 17719641 17719759 17719641 ID=MsaT001278.1.exon2;Parent=MsaT001278.1
Chr1 CDS 17719641 17719759 17719641 ID=cds.MsaT001278.1;Parent=MsaT001278.1
Chr1 exon 17720520 17720647 17720520 ID=MsaT001278.1.exon3;Parent=MsaT001278.1
Chr1 CDS 17720520 17720647 17720520 ID=cds.MsaT001278.1;Parent=MsaT001278.1
Chr1 exon 17720731 17721207 17720731 ID=MsaT001278.1.exon4;Parent=MsaT001278.1
Chr1 CDS 17720731 17721207 17720731 ID=cds.MsaT001278.1;Parent=MsaT001278.1
Chr1 exon 17721295 17721441 17721295 ID=MsaT001278.1.exon5;Parent=MsaT001278.1
Chr1 CDS 17721295 17721441 17721295 ID=cds.MsaT001278.1;Parent=MsaT001278.1
Gene Sequence

>MsaT001278.1

ATGATCAATCAATGGAACCCACCGCCTCCGCCGCTAAACACCTCGAAAACACCATCCCTTACTGCGGCAGAAGAATCACCTCCGCTGCCCATCACCAATCGTAAAGATATTGTCAGAGAGAAGAGTGAGATGGAACCAAGTGTTGTTGTTGCTAGAAAAGTTCAGAAGGCTGACCGTGAGAAAATCAGGAGGGATCGTTTAAATGATCATTTTCATCAATTGGGAAACGTAATAGATCCTGATAGACCACGGAATGACAAGGCAACCATCATCACAGAGACAATTCAAGTGCTAAAGGACATAACATCTGAAGTTGATAGACTGAAAACAGAGCATAAATCACTTTCTGAGGAGTCACATGAACTAATACAAGAAAAGAATGAACTCCGAGAAGAGAAAGCGTCTTTAAAATCCGATATAGAAAATCTTAATTCTCAGTACCAGCAGAGAGTCAGAGCCATGCCACCATGGACTGCAATTGACCATTCTGTTGTCATGAGTAGCCCTTATACGTACCCCGTTCCTATGCCAATGCCAATCCCTCCTGGTCCGGTTTCCATTCACCCACCTATGCAACCTTTTCCTTACTATGGAAACCAAAATCCTGGTCATATTCCAAGTCTATCCTCAATGTATATTCCATTCTCGGCTCCAGCTATCCCTCCAATAGAGATGCCATCAGCTCAGTATCCTTCTACATCCCACGTGTTTAGTCGAAAAGAGTCTCGAAGCAAGTCACCAGGTCATAGAAGAAGACCTAGTGATGCAGAAAGATGTAGTGTGTCTCCAGATGTGGCAACAGAGCTTGAGCTTAAGATGCCTGGATCATCAACACAACAGGATTGCACATCTAGAGGAAAAAAGGCAAAGCATTCGGTGATGAGTGATAGAATCATCATAGATGAGAGTGCTTCGAGCCAGTATTCTGCATCTCAGGGACTTCAAGATAGCTCCAACAGCGTCAGCAACACTCCAAAGGCTAGTTAA

Protein sequence

>MsaT001278.1

MINQWNPPPPPLNTSKTPSLTAAEESPPLPITNRKDIVREKSEMEPSVVVARKVQKADREKIRRDRLNDHFHQLGNVIDPDRPRNDKATIITETIQVLKDITSEVDRLKTEHKSLSEESHELIQEKNELREEKASLKSDIENLNSQYQQRVRAMPPWTAIDHSVVMSSPYTYPVPMPMPIPPGPVSIHPPMQPFPYYGNQNPGHIPSLSSMYIPFSAPAIPPIEMPSAQYPSTSHVFSRKESRSKSPGHRRRPSDAERCSVSPDVATELELKMPGSSTQQDCTSRGKKAKHSVMSDRIIIDESASSQYSASQGLQDSSNSVSNTPKAS*