Alfalfa Gene Editing Database
Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|---|---|---|---|---|---|---|---|---|---|---|
MsaG001296 | KEH40658.1 | 87.273 | 110 | 12 | 1 | 3 | 112 | 58 | 165 | 1.19e-60 | 194 |
Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|
Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|---|---|---|---|---|---|---|---|---|---|---|
MsaG001296 | tr|A0A072VGU3|A0A072VGU3_MEDTR | 87.273 | 110 | 12 | 1 | 3 | 112 | 58 | 165 | 5.71e-61 | 194 |
Gene ID | Type | Classification |
---|---|---|
MsaG001296 | TF | PLATZ |
Gene ID | Type | Classification |
---|
Co-expression Network
Gene1 | Gene2 | correlation coefficient | p_value | FDR |
---|---|---|---|---|
MsaG000118 | MsaG001296 | 0.806944 | 1.091365e-49 | 1.731823e-47 |
MsaG000153 | MsaG001296 | 0.804974 | 2.819958e-49 | 4.272677e-47 |
MsaG000523 | MsaG001296 | 0.813305 | 4.716940e-51 | 8.737834e-49 |
MsaG001027 | MsaG001296 | 0.825474 | 8.162832e-54 | 2.080287e-51 |
MsaG001296 | MsaG002350 | 0.833302 | 1.045351e-55 | 3.329353e-53 |
MsaG001296 | MsaG002528 | 0.800760 | 2.073312e-48 | 2.851806e-46 |
MsaG001296 | MsaG003289 | 0.816638 | 8.664295e-52 | 1.746015e-49 |
MsaG001296 | MsaG003557 | 0.808627 | 4.808399e-50 | 7.943944e-48 |
MsaG001296 | MsaG003834 | 0.809184 | 3.659967e-50 | 6.128120e-48 |
MsaG001296 | MsaG004297 | 0.813139 | 5.126136e-51 | 9.456976e-49 |
MsaG001296 | MsaG004428 | 0.817222 | 6.416275e-52 | 1.312682e-49 |
MsaG001296 | MsaG004432 | 0.811385 | 1.232960e-50 | 2.177986e-48 |
MsaG001296 | MsaG004656 | 0.817845 | 4.650158e-52 | 9.667121e-50 |
MsaG001296 | MsaG004757 | 0.818081 | 4.114564e-52 | 8.606025e-50 |
MsaG001296 | MsaG004849 | 0.812649 | 6.555839e-51 | 1.194743e-48 |
MsaG001296 | MsaG004913 | 0.857989 | 2.186286e-62 | 1.575845e-59 |
MsaG001296 | MsaG005379 | 0.823203 | 2.775523e-53 | 6.648681e-51 |
MsaG001296 | MsaG005641 | 0.811903 | 9.521396e-51 | 1.703495e-48 |
MsaG001296 | MsaG006072 | 0.835897 | 2.345265e-56 | 8.069554e-54 |
MsaG001296 | MsaG006127 | 0.840004 | 2.085546e-57 | 8.142505e-55 |
MsaG001296 | MsaG006240 | 0.801074 | 1.789686e-48 | 2.479084e-46 |
MsaG001296 | MsaG006318 | 0.815057 | 1.943173e-51 | 3.761696e-49 |
MsaG001296 | MsaG006517 | 0.807720 | 7.488184e-50 | 1.210430e-47 |
MsaG001296 | MsaG006798 | 0.838952 | 3.899994e-57 | 1.473411e-54 |
MsaG001296 | MsaG006979 | 0.806371 | 1.440471e-49 | 2.255176e-47 |
MsaG001296 | MsaG007080 | 0.802828 | 7.838290e-49 | 1.130017e-46 |
MsaG001296 | MsaG007175 | 0.832886 | 1.325576e-55 | 4.170484e-53 |
MsaG001296 | MsaG007291 | 0.801842 | 1.247988e-48 | 1.759146e-46 |
MsaG001296 | MsaG007601 | 0.819117 | 2.401251e-52 | 5.159931e-50 |
MsaG001296 | MsaG007990 | 0.803700 | 5.182849e-49 | 7.624173e-47 |
MsaG001296 | MsaG008412 | 0.827008 | 3.535459e-54 | 9.402651e-52 |
MsaG001296 | MsaG008682 | 0.803255 | 6.401502e-49 | 9.320434e-47 |
MsaG001296 | MsaG008863 | 0.802110 | 1.099870e-48 | 1.559795e-46 |
MsaG001296 | MsaG009261 | 0.826483 | 4.712019e-54 | 1.235071e-51 |
MsaG001296 | MsaG009764 | 0.809203 | 3.625684e-50 | 6.073414e-48 |
MsaG001296 | MsaG010328 | 0.808493 | 5.135461e-50 | 8.456875e-48 |
MsaG001296 | MsaG010372 | 0.801458 | 1.495236e-48 | 2.089315e-46 |
MsaG001296 | MsaG011134 | 0.815475 | 1.571217e-51 | 3.073950e-49 |
MsaG001296 | MsaG011439 | 0.831828 | 2.416018e-55 | 7.371397e-53 |
MsaG001296 | MsaG011508 | 0.813297 | 4.735172e-51 | 8.769931e-49 |
MsaG001296 | MsaG012081 | 0.824401 | 1.458476e-53 | 3.609265e-51 |
MsaG001296 | MsaG012497 | 0.825427 | 8.372412e-54 | 2.130939e-51 |
MsaG001296 | MsaG012498 | 0.801001 | 1.852660e-48 | 2.562084e-46 |
MsaG001296 | MsaG012528 | 0.812012 | 9.018957e-51 | 1.617876e-48 |
MsaG001296 | MsaG013230 | 0.805925 | 1.786140e-49 | 2.766931e-47 |
MsaG001296 | MsaG013266 | 0.805466 | 2.227155e-49 | 3.413203e-47 |
MsaG001296 | MsaG013884 | 0.806429 | 1.400149e-49 | 2.195143e-47 |
MsaG001296 | MsaG014808 | 0.800499 | 2.341845e-48 | 3.202189e-46 |
MsaG001296 | MsaG015446 | 0.801318 | 1.596283e-48 | 2.223475e-46 |
MsaG001296 | MsaG015853 | 0.830835 | 4.227580e-55 | 1.253085e-52 |
MsaG001296 | MsaG016250 | 0.811878 | 9.643579e-51 | 1.724262e-48 |
MsaG001296 | MsaG016406 | 0.831246 | 3.356582e-55 | 1.006702e-52 |
MsaG001296 | MsaG016946 | 0.827391 | 2.864924e-54 | 7.701992e-52 |
MsaG001296 | MsaG017023 | 0.831244 | 3.358999e-55 | 1.007391e-52 |
MsaG001296 | MsaG017083 | 0.817954 | 4.396022e-52 | 9.164533e-50 |
MsaG001296 | MsaG017378 | 0.832269 | 1.882341e-55 | 5.816639e-53 |
MsaG001296 | MsaG017504 | 0.808640 | 4.777663e-50 | 7.895690e-48 |
MsaG001296 | MsaG017839 | 0.813072 | 5.303617e-51 | 9.768137e-49 |
MsaG001296 | MsaG017907 | 0.804155 | 4.172218e-49 | 6.202712e-47 |
MsaG001296 | MsaG017888 | 0.810321 | 2.090104e-50 | 3.597344e-48 |
MsaG001296 | MsaG018006 | 0.810314 | 2.097354e-50 | 3.609210e-48 |
MsaG001296 | MsaG018069 | 0.800451 | 2.395533e-48 | 3.272077e-46 |
MsaG001296 | MsaG018259 | 0.808301 | 5.639748e-50 | 9.244557e-48 |
MsaG001296 | MsaG018484 | 0.804251 | 3.984649e-49 | 5.937076e-47 |
MsaG001296 | MsaG018876 | 0.825037 | 1.034361e-53 | 2.604584e-51 |
MsaG001296 | MsaG018897 | 0.808752 | 4.523268e-50 | 7.495704e-48 |
MsaG001296 | MsaG019122 | 0.803697 | 5.189152e-49 | 7.632961e-47 |
MsaG001296 | MsaG019589 | 0.812478 | 7.142992e-51 | 1.296266e-48 |
MsaG001296 | MsaG019676 | 0.810359 | 2.050868e-50 | 3.533177e-48 |
MsaG001296 | MsaG020712 | 0.833576 | 8.941285e-56 | 2.870750e-53 |
MsaG001296 | MsaG020885 | 0.841257 | 9.831185e-58 | 3.992802e-55 |
MsaG001296 | MsaG021557 | 0.803537 | 5.598425e-49 | 8.204632e-47 |
MsaG001296 | MsaG022112 | 0.850376 | 3.367992e-60 | 1.848918e-57 |
MsaG001296 | MsaG022782 | 0.817526 | 5.484805e-52 | 1.130888e-49 |
MsaG001296 | MsaG023415 | 0.803298 | 6.270927e-49 | 9.139409e-47 |
MsaG001296 | MsaG023475 | 0.801525 | 1.448439e-48 | 2.027027e-46 |
MsaG001296 | MsaG023663 | 0.829521 | 8.820031e-55 | 2.517780e-52 |
MsaG001296 | MsaG023689 | 0.813355 | 4.600112e-51 | 8.532238e-49 |
MsaG001296 | MsaG023692 | 0.810342 | 2.067780e-50 | 3.560885e-48 |
MsaG001296 | MsaG023929 | 0.810562 | 1.854758e-50 | 3.211079e-48 |
MsaG001296 | MsaG026867 | 0.847584 | 1.991167e-59 | 9.943528e-57 |
MsaG001296 | MsaG027260 | 0.813674 | 3.915436e-51 | 7.320893e-49 |
MsaG001296 | MsaG027469 | 0.825408 | 8.462418e-54 | 2.152660e-51 |
MsaG001296 | MsaG027743 | 0.807330 | 9.050457e-50 | 1.449353e-47 |
MsaG001296 | MsaG027863 | 0.843958 | 1.901203e-58 | 8.417746e-56 |
MsaG001296 | MsaG028254 | 0.821897 | 5.564444e-53 | 1.286874e-50 |
MsaG001296 | MsaG028292 | 0.808982 | 4.041109e-50 | 6.733677e-48 |
MsaG001296 | MsaG028516 | 0.829429 | 9.283568e-55 | 2.643133e-52 |
MsaG001296 | MsaG028664 | 0.815064 | 1.936870e-51 | 3.750143e-49 |
MsaG001296 | MsaG028754 | 0.842784 | 3.898432e-58 | 1.662403e-55 |
MsaG001296 | MsaG028883 | 0.805126 | 2.621769e-49 | 3.986491e-47 |
MsaG001296 | MsaG029075 | 0.803610 | 5.407986e-49 | 7.938668e-47 |
MsaG001296 | MsaG030605 | 0.803343 | 6.139259e-49 | 8.956769e-47 |
MsaG001296 | MsaG030781 | 0.832270 | 1.880977e-55 | 5.812661e-53 |
MsaG001296 | MsaG030996 | 0.807414 | 8.687175e-50 | 1.393978e-47 |
MsaG001296 | MsaG033221 | 0.806642 | 1.263568e-49 | 1.990929e-47 |
MsaG001296 | MsaG033975 | 0.841221 | 1.004540e-57 | 4.075234e-55 |
MsaG001296 | MsaG034016 | 0.829217 | 1.044602e-54 | 2.955804e-52 |
MsaG001296 | MsaG034151 | 0.815936 | 1.241169e-51 | 2.456897e-49 |
MsaG001296 | MsaG034778 | 0.824288 | 1.549552e-53 | 3.822934e-51 |
MsaG001296 | MsaG034839 | 0.804143 | 4.196474e-49 | 6.236940e-47 |
MsaG001296 | MsaG035267 | 0.806877 | 1.127766e-49 | 1.786746e-47 |
MsaG001296 | MsaG039682 | 0.836883 | 1.319763e-56 | 4.677895e-54 |
MsaG001296 | MsaG040014 | 0.800479 | 2.364138e-48 | 3.231261e-46 |
MsaG001296 | MsaG040052 | 0.814563 | 2.497836e-51 | 4.775499e-49 |
MsaG001296 | MsaG040474 | 0.803265 | 6.370538e-49 | 9.277510e-47 |
MsaG001296 | MsaG040566 | 0.802616 | 8.662512e-49 | 1.242858e-46 |
MsaG001296 | MsaG041141 | 0.820460 | 1.188772e-52 | 2.646622e-50 |
MsaG001296 | MsaG041224 | 0.803951 | 4.597759e-49 | 6.803051e-47 |
MsaG001296 | MsaG042071 | 0.804416 | 3.683193e-49 | 5.508814e-47 |
MsaG001296 | MsaG042087 | 0.811018 | 1.479406e-50 | 2.589914e-48 |
MsaG001296 | MsaG042684 | 0.800944 | 1.902308e-48 | 2.627386e-46 |
MsaG001296 | MsaG042674 | 0.814911 | 2.093878e-51 | 4.038507e-49 |
MsaG001296 | MsaG043815 | 0.838356 | 5.552009e-57 | 2.058669e-54 |
MsaG001296 | MsaG044048 | 0.805148 | 2.594604e-49 | 3.947179e-47 |
MsaG001296 | MsaG044049 | 0.807395 | 8.768452e-50 | 1.406364e-47 |
MsaG001296 | MsaG044617 | 0.803936 | 4.631452e-49 | 6.850384e-47 |
MsaG001296 | MsaG044623 | 0.808021 | 6.465031e-50 | 1.052653e-47 |
MsaG001296 | MsaG044938 | 0.801303 | 1.607746e-48 | 2.238640e-46 |
MsaG001296 | MsaG045060 | 0.811359 | 1.248732e-50 | 2.204453e-48 |
MsaG001296 | MsaG045061 | 0.832837 | 1.362727e-55 | 4.281095e-53 |
MsaG001296 | MsaG045062 | 0.806032 | 1.696113e-49 | 2.634100e-47 |
MsaG001296 | MsaG045145 | 0.825069 | 1.016635e-53 | 2.562253e-51 |
MsaG001296 | MsaG045290 | 0.811355 | 1.251115e-50 | 2.208448e-48 |
MsaG001296 | MsaG045535 | 0.829070 | 1.133455e-54 | 3.194098e-52 |
MsaG001296 | MsaG045672 | 0.800637 | 2.195662e-48 | 3.011783e-46 |
MsaG001296 | MsaG045952 | 0.800321 | 2.545729e-48 | 3.466909e-46 |
MsaG001296 | MsaG046113 | 0.807929 | 6.761589e-50 | 1.098520e-47 |
MsaG001296 | MsaG046510 | 0.820056 | 1.469506e-52 | 3.236853e-50 |
MsaG001296 | MsaG046701 | 0.801960 | 1.180818e-48 | 1.668901e-46 |
MsaG001296 | MsaG046932 | 0.810302 | 2.109079e-50 | 3.628346e-48 |
MsaG001296 | MsaG046944 | 0.823692 | 2.135495e-53 | 5.184046e-51 |
MsaG001296 | MsaG047030 | 0.808793 | 4.433581e-50 | 7.354019e-48 |
MsaG001296 | MsaG047139 | 0.815387 | 1.642964e-51 | 3.207249e-49 |
MsaG001296 | MsaG001530 | 0.814607 | 2.443409e-51 | 4.676511e-49 |
MsaG001296 | MsaG002774 | 0.807453 | 8.526845e-50 | 1.369498e-47 |
MsaG001296 | MsaG001894 | 0.804833 | 3.018131e-49 | 4.557983e-47 |
MsaG001296 | MsaG002369 | 0.800904 | 1.937903e-48 | 2.674133e-46 |
MsaG001296 | MsaG003779 | 0.803693 | 5.198619e-49 | 7.646167e-47 |
MsaG001296 | MsaG000843 | 0.824499 | 1.383138e-53 | 3.432056e-51 |
MsaG001296 | MsaG008672 | 0.807235 | 9.478421e-50 | 1.514419e-47 |
MsaG001296 | MsaG008139 | 0.811873 | 9.668201e-51 | 1.728444e-48 |
MsaG001296 | MsaG013545 | 0.801799 | 1.273756e-48 | 1.793701e-46 |
MsaG001296 | MsaG011821 | 0.802405 | 9.569465e-49 | 1.366392e-46 |
MsaG001296 | MsaG018000 | 0.831107 | 3.628087e-55 | 1.083835e-52 |
MsaG001296 | MsaG026455 | 0.828056 | 1.987322e-54 | 5.443032e-52 |
MsaG001296 | MsaG026065 | 0.800217 | 2.671597e-48 | 3.629803e-46 |
MsaG001296 | MsaG026813 | 0.820680 | 1.058976e-52 | 2.371192e-50 |
MsaG001296 | MsaG033056 | 0.819996 | 1.516512e-52 | 3.335079e-50 |
MsaG001296 | MsaG033077 | 0.804765 | 3.117547e-49 | 4.700655e-47 |
MsaG001296 | MsaG030575 | 0.801707 | 1.330070e-48 | 1.869048e-46 |
MsaG001296 | MsaG031607 | 0.802450 | 9.369557e-49 | 1.339201e-46 |
MsaG001296 | MsaG036797 | 0.828484 | 1.568823e-54 | 4.348965e-52 |
MsaG001296 | MsaG036198 | 0.804221 | 4.043364e-49 | 6.020322e-47 |
MsaG001296 | MsaG035293 | 0.805918 | 1.791645e-49 | 2.775029e-47 |
MsaG001296 | MsaG034924 | 0.800129 | 2.783653e-48 | 3.774539e-46 |
MsaG001296 | MsaG037381 | 0.807863 | 6.984090e-50 | 1.132850e-47 |
MsaG001296 | MsaG036432 | 0.803312 | 6.229201e-49 | 9.081633e-47 |
MsaG001296 | MsaG036880 | 0.819672 | 1.796770e-52 | 3.917412e-50 |
MsaG001296 | MsaG036535 | 0.836293 | 1.862623e-56 | 6.486055e-54 |
MsaG001296 | MsaG038171 | 0.800690 | 2.142559e-48 | 2.942456e-46 |
MsaG001296 | MsaG045038 | 0.832197 | 1.960268e-55 | 6.044910e-53 |
MsaG001296 | MsaG040992 | 0.803988 | 4.517328e-49 | 6.689693e-47 |
PPI
Gene1 | Gene2 | Type |
---|
Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|---|---|---|---|---|---|---|---|---|---|---|
MsaG001296 | MtrunA17_Chr8g0392151 | 37.255 | 153 | 50 | 2 | 3 | 109 | 88 | 240 | 1.26e-23 | 91.3 |
Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|
Find 20 sgRNAs with CRISPR-Local
Find sgRNAs with CRISPR-GE
CRISPR-Local
sgRNA_sequence | on_target_score | Position | Region |
---|---|---|---|
ATGACTCAGCCTCTGAATTT+TGG | 0.202823 | 1:-18096006 | None:intergenic |
ATGTATAAGTTGGGTGTTTA+TGG | 0.217859 | 1:+18096266 | MsaT001296.1:intron |
TGTTGTTACCTTGCATTTGA+TGG | 0.328798 | 1:-18095290 | None:intergenic |
CCACTCATATCAAAGCTAAA+TGG | 0.332015 | 1:-18096290 | None:intergenic |
TGTCTTTGCTACTGCTAGTC+GGG | 0.379634 | 1:-18095188 | None:intergenic |
ACCCTTTCAACAGATTCATT+TGG | 0.404698 | 1:-18096046 | None:intergenic |
TAGATCACAATTGAGGAGCT+TGG | 0.458055 | 1:+18095965 | MsaT001296.1:intron |
ATGTCTTTGCTACTGCTAGT+CGG | 0.459833 | 1:-18095189 | None:intergenic |
AAACTAGTGATAGATCTCAT+AGG | 0.499860 | 1:-18096100 | None:intergenic |
AACCAAATGAATCTGTTGAA+AGG | 0.544922 | 1:+18096044 | MsaT001296.1:CDS |
ACCAAATGAATCTGTTGAAA+GGG | 0.545715 | 1:+18096045 | MsaT001296.1:CDS |
CCATTTAGCTTTGATATGAG+TGG | 0.563263 | 1:+18096290 | MsaT001296.1:CDS |
GTATTGTTAGATCACAATTG+AGG | 0.590386 | 1:+18095958 | MsaT001296.1:intron |
ACATCTCAAAAGCAAGCTGG+TGG | 0.603751 | 1:+18095214 | MsaT001296.1:CDS |
TCAACATCTCAAAAGCAAGC+TGG | 0.604147 | 1:+18095211 | MsaT001296.1:CDS |
GTCTTTGCTACTGCTAGTCG+GGG | 0.609471 | 1:-18095187 | None:intergenic |
ATTCTGTTCCATCAAATGCA+AGG | 0.622156 | 1:+18095282 | MsaT001296.1:CDS |
TGAATCTCAGAAAACGTCCA+AGG | 0.636921 | 1:+18096068 | MsaT001296.1:CDS |
AAATGATCACCAAAATTCAG+AGG | 0.644619 | 1:+18095997 | MsaT001296.1:CDS |
GGTGGAATTTGTGAAGAATG+TGG | 0.715683 | 1:+18095232 | MsaT001296.1:CDS |
CRISPR-GE
badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
---|
Chromosome | Type | Strat | End | Strand | Name |
---|---|---|---|---|---|
Chr1 | gene | 18095136 | 18096331 | 18095136 | ID=MsaG001296 |
Chr1 | mRNA | 18095136 | 18096331 | 18095136 | ID=MsaT001296.1;Parent=MsaG001296 |
Chr1 | exon | 18095136 | 18095303 | 18095136 | ID=MsaT001296.1.exon1;Parent=MsaT001296.1 |
Chr1 | CDS | 18095136 | 18095303 | 18095136 | ID=cds.MsaT001296.1;Parent=MsaT001296.1 |
Chr1 | exon | 18095968 | 18096123 | 18095968 | ID=MsaT001296.1.exon2;Parent=MsaT001296.1 |
Chr1 | CDS | 18095968 | 18096123 | 18095968 | ID=cds.MsaT001296.1;Parent=MsaT001296.1 |
Chr1 | exon | 18096275 | 18096331 | 18096275 | ID=MsaT001296.1.exon3;Parent=MsaT001296.1 |
Chr1 | CDS | 18096275 | 18096331 | 18096275 | ID=cds.MsaT001296.1;Parent=MsaT001296.1 |
Gene Sequence |
Protein sequence |