AlfalfaGEDB | MsaG001336

M. sativa ssp. caerulea / MsaG001336

KEGG: -
GO: -
PFAM: PF00249.34

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG001336	KEH40705.1	98.519	135	2	0	1	135	1	135	2.49e-93	276

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG001336	sp\|Q9FNN6\|SRM1_ARATH	62.044	137	47	5	1	137	1	132	8.83e-48	159

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG001336	tr\|A0A072VRE4\|A0A072VRE4_MEDTR	98.519	135	2	0	1	135	1	135	1.19e-93	276

TFs/TRs:

Gene ID	Type	Classification
MsaG001336	TF	MYB-related

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsaG000164	MsaG001336	0.813512	4.249125e-51	7.912719e-49
MsaG000976	MsaG001336	0.807111	1.006692e-49	1.603764e-47
MsaG001336	MsaG001403	0.821661	6.306119e-53	1.449304e-50
MsaG001336	MsaG002201	0.800353	2.507688e-48	3.417698e-46
MsaG001336	MsaG002354	0.809241	3.558727e-50	5.966699e-48
MsaG001336	MsaG002359	0.805271	2.446179e-49	3.731911e-47
MsaG001336	MsaG003188	0.869158	7.740131e-66	8.658452e-63
MsaG001336	MsaG005058	0.820736	1.028199e-52	2.305735e-50
MsaG001336	MsaG005201	0.825367	8.651028e-54	2.198275e-51
MsaG001336	MsaG005533	0.802970	7.327851e-49	1.059907e-46
MsaG001336	MsaG006048	0.808085	6.267899e-50	1.022098e-47
MsaG001336	MsaG006124	0.804225	4.034621e-49	6.007952e-47
MsaG001336	MsaG007912	0.807690	7.597707e-50	1.227243e-47
MsaG001336	MsaG009174	0.804477	3.577909e-49	5.358844e-47
MsaG001336	MsaG009230	0.807800	7.199779e-50	1.166068e-47
MsaG001336	MsaG009759	0.801575	1.414876e-48	1.982303e-46
MsaG001336	MsaG010466	0.803453	5.828483e-49	8.524932e-47
MsaG001336	MsaG011226	0.805727	1.964598e-49	3.029306e-47
MsaG001336	MsaG012262	0.807441	8.575334e-50	1.376911e-47
MsaG001336	MsaG012736	0.809535	3.079950e-50	5.201049e-48
MsaG001336	MsaG014337	0.818819	2.805109e-52	5.980552e-50
MsaG001336	MsaG014897	0.804997	2.789083e-49	4.228196e-47
MsaG001336	MsaG016540	0.816896	7.586373e-52	1.539065e-49
MsaG001336	MsaG016835	0.856985	4.320586e-62	2.999953e-59
MsaG001336	MsaG017355	0.849997	4.293724e-60	2.326918e-57
MsaG001336	MsaG017356	0.820207	1.357369e-52	3.001949e-50
MsaG001336	MsaG019191	0.811140	1.392343e-50	2.444811e-48
MsaG001336	MsaG019571	0.813254	4.839353e-51	8.953489e-49
MsaG001336	MsaG019640	0.808175	5.998280e-50	9.802638e-48
MsaG001336	MsaG020724	0.808821	4.372511e-50	7.257817e-48
MsaG001336	MsaG021085	0.806065	1.669825e-49	2.595346e-47
MsaG001336	MsaG022607	0.807761	7.338673e-50	1.187437e-47
MsaG001336	MsaG022979	0.830687	4.594646e-55	1.356062e-52
MsaG001336	MsaG025475	0.822217	4.694959e-53	1.095142e-50
MsaG001336	MsaG025598	0.841384	9.103866e-58	3.712348e-55
MsaG001336	MsaG027172	0.857379	3.309634e-62	2.332025e-59
MsaG001336	MsaG027177	0.837251	1.063737e-56	3.812718e-54
MsaG001336	MsaG027359	0.813855	3.574797e-51	6.714467e-49
MsaG001336	MsaG027692	0.820064	1.463585e-52	3.224481e-50
MsaG001336	MsaG027747	0.802452	9.361516e-49	1.338112e-46
MsaG001336	MsaG029366	0.821760	5.983284e-53	1.378709e-50
MsaG001336	MsaG029680	0.819700	1.771270e-52	3.864635e-50
MsaG001336	MsaG030592	0.869684	5.228529e-66	5.979452e-63
MsaG001336	MsaG031057	0.811210	1.344997e-50	2.365739e-48
MsaG001336	MsaG033312	0.802975	7.309082e-49	1.057343e-46
MsaG001336	MsaG033451	0.822961	3.158248e-53	7.516362e-51
MsaG001336	MsaG033632	0.813413	4.466308e-51	8.296436e-49
MsaG001336	MsaG033776	0.831979	2.218612e-55	6.798538e-53
MsaG001336	MsaG034851	0.803762	5.031521e-49	7.412224e-47
MsaG001336	MsaG035118	0.801327	1.589906e-48	2.215045e-46
MsaG001336	MsaG035642	0.803221	6.506422e-49	9.465854e-47
MsaG001336	MsaG035666	0.820497	1.165647e-52	2.597657e-50
MsaG001336	MsaG035804	0.800841	1.996076e-48	2.750543e-46
MsaG001336	MsaG035867	0.810283	2.129083e-50	3.661051e-48
MsaG001336	MsaG036577	0.833061	1.199334e-55	3.792850e-53
MsaG001336	MsaG036844	0.834610	4.936956e-56	1.634557e-53
MsaG001336	MsaG039930	0.865204	1.399035e-64	1.332703e-61
MsaG001336	MsaG041599	0.819366	2.108549e-52	4.560726e-50
MsaG001336	MsaG042767	0.802320	9.964663e-49	1.420034e-46
MsaG001336	MsaG045612	0.804024	4.441692e-49	6.583336e-47
MsaG001336	MsaG045922	0.808471	5.189603e-50	8.541738e-48
MsaG001336	MsaG046214	0.816061	1.164296e-51	2.311985e-49
MsaG001336	MsaG046456	0.815268	1.746165e-51	3.398486e-49
MsaG001336	MsaG046562	0.820100	1.436347e-52	3.167535e-50
MsaG001336	MsaG013062	0.813631	4.002144e-51	7.474881e-49
MsaG001336	MsaG012599	0.824229	1.600047e-53	3.941060e-51
MsaG001336	MsaG011535	0.854274	2.648025e-61	1.667129e-58
MsaG001336	MsaG012697	0.818645	3.070350e-52	6.516452e-50
MsaG001336	MsaG019705	0.805739	1.953307e-49	3.012742e-47
MsaG001336	MsaG028385	0.801606	1.394223e-48	1.954777e-46
MsaG001336	MsaG026444	0.801720	1.321937e-48	1.858202e-46
MsaG001336	MsaG034616	0.845926	5.628314e-59	2.659071e-56
MsaG001336	MsaG031512	0.826564	4.507847e-54	1.184183e-51
MsaG001336	MsaG031279	0.803633	5.348210e-49	7.855245e-47
MsaG001336	MsaG033287	0.821367	7.367481e-53	1.680047e-50
MsaG001336	MsaG037361	0.862150	1.228963e-63	1.037449e-60
MsaG001336	MsaG037217	0.816898	7.578633e-52	1.537579e-49
MsaG001336	MsaG042835	0.803972	4.553450e-49	6.740656e-47
MsaG001336	MsaG046774	0.819353	2.123626e-52	4.591687e-50

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG001336	MtrunA17_Chr1g0162081	95.620	137	5	1	1	137	1	136	5.35e-94	273
MsaG001336	MtrunA17_Chr8g0364741	60.305	131	38	1	3	133	1	117	7.93e-48	150
MsaG001336	MtrunA17_Chr8g0365011	52.344	128	33	3	3	130	1	100	4.36e-30	110
MsaG001336	MtrunA17_Chr4g0062741	45.890	146	58	6	12	137	8	152	1.68e-28	106
MsaG001336	MtrunA17_Chr3g0104071	38.060	134	76	2	4	137	35	161	1.88e-28	106
MsaG001336	MtrunA17_Chr7g0241761	41.007	139	60	3	7	137	30	154	6.90e-27	102
MsaG001336	MtrunA17_Chr1g0155181	51.807	83	40	0	6	88	1	83	2.74e-25	92.0
MsaG001336	MtrunA17_Chr1g0210471	43.939	132	55	3	10	137	22	138	4.33e-25	95.9
MsaG001336	MtrunA17_Chr5g0436501	43.750	128	59	5	10	137	25	139	4.33e-25	96.7
MsaG001336	MtrunA17_Chr5g0429521	38.060	134	65	3	9	137	26	146	1.99e-23	92.8
MsaG001336	MtrunA17_Chr3g0141601	55.072	69	31	0	9	77	4	72	3.15e-21	82.0
MsaG001336	MtrunA17_Chr3g0144631	50.000	68	34	0	10	77	14	81	3.36e-20	79.0
MsaG001336	MtrunA17_Chr5g0404101	48.684	76	39	0	7	82	14	89	6.32e-20	79.0
MsaG001336	MtrunA17_Chr7g0255841	56.250	64	28	0	15	78	15	78	2.19e-19	77.4
MsaG001336	MtrunA17_Chr6g0449111	50.685	73	36	0	3	75	1	73	2.94e-19	76.6
MsaG001336	MtrunA17_Chr3g0141641	41.791	67	39	0	12	78	3	69	1.18e-16	70.1
MsaG001336	MtrunA17_Chr8g0373131	39.726	73	43	1	9	81	2	73	5.52e-16	68.2
MsaG001336	MtrunA17_Chr8g0373111	40.000	70	41	1	9	78	2	70	1.53e-15	67.0
MsaG001336	MtrunA17_Chr8g0373121	40.000	70	41	1	9	78	2	70	1.53e-15	67.0
MsaG001336	MtrunA17_Chr3g0081631	45.205	73	39	1	3	74	1	73	2.74e-15	66.6
MsaG001336	MtrunA17_Chr8g0373101	42.857	63	35	1	10	72	3	64	1.63e-13	61.6
MsaG001336	MtrunA17_Chr5g0404301	46.032	63	33	1	10	72	2	63	3.56e-13	60.5

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG001336	AT5G08520.1	62.044	137	47	5	1	137	1	132	8.97e-49	159
MsaG001336	AT5G05790.2	43.077	130	61	4	9	137	28	145	9.44e-26	99.0
MsaG001336	AT5G05790.1	43.077	130	61	4	9	137	28	145	9.44e-26	99.0
MsaG001336	AT2G38090.1	39.231	130	76	2	10	137	27	155	1.89e-25	98.6
MsaG001336	AT3G11280.2	40.945	127	56	3	12	137	33	141	8.06e-25	96.3
MsaG001336	AT3G11280.1	40.945	127	56	3	12	137	33	141	8.06e-25	96.3
MsaG001336	AT5G58900.1	40.000	130	70	4	10	137	32	155	3.49e-24	94.7
MsaG001336	AT5G23650.1	41.732	127	71	2	5	131	6	129	6.26e-24	95.1
MsaG001336	AT4G39250.1	51.948	77	37	0	12	88	14	90	7.23e-23	86.7
MsaG001336	AT1G49010.1	39.310	145	69	4	10	137	6	148	7.32e-23	92.0
MsaG001336	AT1G75250.2	50.000	74	37	0	10	83	10	83	9.51e-22	84.0
MsaG001336	AT1G75250.1	50.000	74	37	0	10	83	10	83	1.91e-21	84.0
MsaG001336	AT2G21650.1	51.429	70	34	0	12	81	14	83	1.33e-18	75.9
MsaG001336	AT1G19510.1	45.946	74	40	0	15	88	15	88	1.43e-18	75.9
MsaG001336	AT5G01200.1	34.286	140	73	5	10	137	28	160	1.13e-17	77.4
MsaG001336	AT2G18328.1	50.794	63	31	0	16	78	15	77	4.23e-17	71.2
MsaG001336	AT5G04760.1	34.109	129	67	4	9	137	3	113	4.87e-16	72.0

Find 47 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTTCAACAAGACTATGACTA+TGG	0.261298	1:+18707082	None:intergenic
AGTAATCAAAATTGGCATAA+AGG	0.323067	1:-18707502	None:intergenic
AAGTTAAAAGTAATCAAAAT+TGG	0.330013	1:-18707510	None:intergenic
GAGCTCTTGGTTGATGATAT+TGG	0.334244	1:+18707257	MsaT001336.1:CDS
CGATGTACCGGGGAAGACTA+TGG	0.416693	1:+18707214	MsaT001336.1:CDS
CGAGTCTAACCATGGAACTA+AGG	0.427854	1:+18707403	MsaT001336.1:CDS
GCCATCGTATAATTCTTCTT+CGG	0.436648	1:+18707307	MsaT001336.1:CDS
ATGCTAGCGATGAAGGAGTT+GGG	0.443412	1:+18707345	MsaT001336.1:CDS
TAAGCAGCATTATGAGCTCT+TGG	0.453346	1:+18707244	MsaT001336.1:CDS
ACAATAGCTCTGAGTGGAGC+TGG	0.456097	1:+18707114	MsaT001336.1:CDS
GAAGATTGCGGCCGATGTAC+CGG	0.462750	1:+18707202	MsaT001336.1:CDS
ACAAGTCATGCTAGCGATGA+AGG	0.470619	1:+18707338	MsaT001336.1:CDS
GCACACAACCAGCCTCAATC+TGG	0.478933	1:-18707280	None:intergenic
ATCGTATAATTCTTCTTCGG+AGG	0.495952	1:+18707310	MsaT001336.1:CDS
CTGAGGATGATTCGGATCGA+TGG	0.497129	1:+18707177	MsaT001336.1:CDS
ATCTTGAGCCCTTAGTTCCA+TGG	0.501596	1:-18707412	None:intergenic
GACTATGACTATGGATGAAG+TGG	0.502959	1:+18707091	None:intergenic
GACGTATCCTGAGGATGATT+CGG	0.503412	1:+18707169	MsaT001336.1:CDS
AAGATTGCGGCCGATGTACC+GGG	0.505203	1:+18707203	MsaT001336.1:CDS
CATGCTAGCGATGAAGGAGT+TGG	0.506146	1:+18707344	MsaT001336.1:CDS
ATTTCTTCCATAGTCTTCCC+CGG	0.518652	1:-18707221	None:intergenic
AACGACGAAAGGGTATTGCT+TGG	0.523028	1:+18707444	MsaT001336.1:CDS
TAAGGCATTTGAAAATGCCT+TGG	0.529052	1:+18707145	MsaT001336.1:CDS
TGATGATATTGGCCAGATTG+AGG	0.549035	1:+18707268	MsaT001336.1:CDS
TGAGGATGATTCGGATCGAT+GGG	0.549268	1:+18707178	MsaT001336.1:CDS
CATCGATCCGAATCATCCTC+AGG	0.553968	1:-18707176	None:intergenic
GATATTGGCCAGATTGAGGC+TGG	0.554254	1:+18707272	MsaT001336.1:CDS
TGCTAGCGATGAAGGAGTTG+GGG	0.556566	1:+18707346	MsaT001336.1:CDS
GGGAAGAAGGGTGGTCACAA+TGG	0.559678	1:+18707368	MsaT001336.1:CDS
ATCTGATCAAGAACGACGAA+AGG	0.561791	1:+18707433	MsaT001336.1:CDS
CAATAGCTCTGAGTGGAGCT+GGG	0.561880	1:+18707115	MsaT001336.1:CDS
TCGTATAATTCTTCTTCGGA+GGG	0.565463	1:+18707311	MsaT001336.1:CDS
TCTGATCAAGAACGACGAAA+GGG	0.570329	1:+18707434	MsaT001336.1:CDS
GTGGAGCTGGGAGCAAGATA+AGG	0.570448	1:+18707127	MsaT001336.1:CDS
CATAGTCTTCCCCGGTACAT+CGG	0.575851	1:-18707213	None:intergenic
GAGTCTAACCATGGAACTAA+GGG	0.584526	1:+18707404	MsaT001336.1:CDS
AAAGGGTATTGCTTGGACTG+AGG	0.609474	1:+18707451	MsaT001336.1:CDS
GAAGAATTATACGATGGCAA+AGG	0.633833	1:-18707302	None:intergenic
GGATCGATGGGAGAAGATTG+CGG	0.637780	1:+18707190	MsaT001336.1:CDS
TGCCTTGGCGACGTATCCTG+AGG	0.642436	1:+18707160	MsaT001336.1:CDS
GCTAGCGATGAAGGAGTTGG+GGG	0.644660	1:+18707347	MsaT001336.1:CDS
ATCCTCAGGATACGTCGCCA+AGG	0.650046	1:-18707162	None:intergenic
AGATTGCGGCCGATGTACCG+GGG	0.676094	1:+18707204	MsaT001336.1:CDS
TCCGAAGAAGAATTATACGA+TGG	0.694503	1:-18707308	None:intergenic
AAGTGGACAATAGCTCTGAG+TGG	0.698553	1:+18707108	MsaT001336.1:CDS
CTTGGACTGAGGAAGAACAC+AGG	0.712971	1:+18707462	MsaT001336.1:CDS
CTAGCGATGAAGGAGTTGGG+GGG	0.726058	1:+18707348	MsaT001336.1:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content

Chromosome	Type	Strat	End	Strand	Name
Chr1	gene	18707095	18707529	18707095	ID=MsaG001336
Chr1	mRNA	18707095	18707529	18707095	ID=MsaT001336.1;Parent=MsaG001336
Chr1	exon	18707095	18707529	18707095	ID=MsaT001336.1.exon1;Parent=MsaT001336.1
Chr1	CDS	18707095	18707529	18707095	ID=cds.MsaT001336.1;Parent=MsaT001336.1

Gene Sequence

>MsaT001336.1

ATGACTATGGATGAAGTGGACAATAGCTCTGAGTGGAGCTGGGAGCAAGATAAGGCATTTGAAAATGCCTTGGCGACGTATCCTGAGGATGATTCGGATCGATGGGAGAAGATTGCGGCCGATGTACCGGGGAAGACTATGGAAGAAATTAAGCAGCATTATGAGCTCTTGGTTGATGATATTGGCCAGATTGAGGCTGGTTGTGTGCCTTTGCCATCGTATAATTCTTCTTCGGAGGGTTCAACAAGTCATGCTAGCGATGAAGGAGTTGGGGGGAAGAAGGGTGGTCACAATGGCCACAAAAACGACGAGTCTAACCATGGAACTAAGGGCTCAAGATCTGATCAAGAACGACGAAAGGGTATTGCTTGGACTGAGGAAGAACACAGGCAAGTTTATCTCTTAAACCTTTATGCCAATTTTGATTACTTTTAA

Protein sequence

>MsaT001336.1

MTMDEVDNSSEWSWEQDKAFENALATYPEDDSDRWEKIAADVPGKTMEEIKQHYELLVDDIGQIEAGCVPLPSYNSSSEGSTSHASDEGVGGKKGGHNGHKNDESNHGTKGSRSDQERRKGIAWTEEEHRQVYLLNLYANFDYF*