AlfalfaGEDB | MsaG001388

M. sativa ssp. caerulea / MsaG001388

KEGG: K09060
GO: GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
PFAM: PF00170.24

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG001388	XP_013466739.1	94.444	306	17	0	1	306	1	306	0.0	602

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG001388	sp\|P42774\|GBF1_ARATH	26.780	295	184	6	22	301	24	301	4.13e-21	94.7

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG001388	tr\|A0A072VRN1\|A0A072VRN1_MEDTR	94.444	306	17	0	1	306	1	306	0.0	602

TFs/TRs:

Gene ID	Type	Classification
MsaG001388	TF	bZIP

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsaG001381	MsaG001388	0.805125	2.623376e-49	3.988806e-47
MsaG001383	MsaG001388	0.835893	2.350493e-56	8.086642e-54
MsaG001385	MsaG001388	0.855226	1.406418e-61	9.159943e-59
MsaG001386	MsaG001388	0.916719	3.183349e-85	4.736198e-81
MsaG001387	MsaG001388	0.853031	6.004020e-61	3.616538e-58
MsaG001388	MsaG001389	0.880631	9.903464e-70	1.841304e-66
MsaG001388	MsaG001390	0.838540	4.979893e-57	1.857328e-54
MsaG001388	MsaG002629	0.823111	2.915463e-53	6.966529e-51
MsaG001388	MsaG006093	0.802573	8.842172e-49	1.267379e-46
MsaG001388	MsaG008435	0.801295	1.613537e-48	2.246331e-46
MsaG001388	MsaG045980	0.806995	1.064664e-49	1.691496e-47
MsaG001388	MsaG046931	0.812153	8.407209e-51	1.513329e-48
MsaG001388	MsaG008516	0.804483	3.568149e-49	5.344898e-47
MsaG001388	MsaG008312	0.800313	2.554849e-48	3.478749e-46
MsaG001388	MsaG031457	0.811776	1.014800e-50	1.809873e-48
MsaG001388	MsaG031431	0.814853	2.155944e-51	4.152221e-49
MsaG001388	MsaG031556	0.812947	5.646292e-51	1.036666e-48
MsaG001388	MsaG036797	0.832545	1.609417e-55	5.013290e-53
MsaG001388	MsaG034787	0.803591	5.457427e-49	8.007854e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG001388	MtrunA17_Chr1g0162721	94.444	306	17	0	1	306	1	306	0.0	602
MsaG001388	MtrunA17_Chr2g0302481	61.574	216	48	5	95	307	5	188	9.68e-77	232
MsaG001388	MtrunA17_Chr2g0302501	50.000	154	29	6	1	152	20	127	1.22e-32	117
MsaG001388	MtrunA17_Chr4g0065261	61.798	89	33	1	219	307	88	175	1.43e-28	108
MsaG001388	MtrunA17_Chr4g0065261	47.778	90	26	2	28	97	1	89	2.80e-19	83.6
MsaG001388	MtrunA17_Chr8g0373571	48.750	80	41	0	222	301	260	339	2.92e-20	89.7
MsaG001388	MtrunA17_Chr5g0410531	36.082	97	60	1	198	294	286	380	2.25e-12	67.0
MsaG001388	MtrunA17_Chr8g0369161	36.709	79	50	0	211	289	296	374	3.53e-11	63.5
MsaG001388	MtrunA17_Chr7g0275321	26.124	356	192	14	3	294	6	354	5.68e-11	62.8

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG001388	AT4G36730.2	25.685	292	189	5	22	301	24	299	3.62e-22	94.7
MsaG001388	AT4G36730.1	26.780	295	184	6	22	301	24	301	4.20e-22	94.7
MsaG001388	AT2G35530.1	41.176	68	40	0	222	289	305	372	1.05e-12	68.6
MsaG001388	AT1G32150.1	31.515	165	95	5	138	289	203	362	4.99e-11	63.5

Find 61 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AACAACTACGTGCGGTTTAT+TGG	0.157646	1:-19368796	None:intergenic
TCTGGTCTGGCTCAGAATTT+AGG	0.161997	1:+19369779	MsaT001388.1:CDS
GAAATGGTACACAGAAATTT+TGG	0.193208	1:+19369097	MsaT001388.1:CDS
GATTTATGCTTCTTAGTTAA+AGG	0.201185	1:-19369578	None:intergenic
ACTGGGGAATCAAGAGAATT+TGG	0.246551	1:+19369178	MsaT001388.1:CDS
TGCAAGTGGATGATTATAAA+AGG	0.250430	1:-19368573	None:intergenic
TCCCCAGTCATTAAACTTAC+AGG	0.268465	1:-19369163	None:intergenic
TAGCAAATAAGTTGAAATCT+TGG	0.305874	1:-19369129	None:intergenic
TGTGGTAGCATAGGTTTGTT+TGG	0.366044	1:-19368396	None:intergenic
CTGGTCTGGCTCAGAATTTA+GGG	0.370245	1:+19369780	MsaT001388.1:CDS
GAAGAGCTAGTTAAGAAGTA+TGG	0.383568	1:+19374264	MsaT001388.1:CDS
AATAGATCTGCAATTGATTC+TGG	0.403345	1:-19374288	None:intergenic
AAACAGGCTTATTACAATCC+TGG	0.410680	1:+19368536	MsaT001388.1:intron
AGCATAGGTTTGTTTGGAGT+TGG	0.420734	1:-19368390	None:intergenic
TCAGTACATCTTGCAAAATC+AGG	0.425666	1:+19368616	MsaT001388.1:CDS
AAGATCAAAGATAAAGAAAC+AGG	0.437513	1:+19371186	MsaT001388.1:CDS
TTCCTTGTAGAAAATCCATC+TGG	0.463105	1:+19369761	MsaT001388.1:intron
TAAGCTTTCTGAGGAATGTC+TGG	0.463695	1:+19372337	MsaT001388.1:CDS
GCAAGTGGATGATTATAAAA+GGG	0.464969	1:-19368572	None:intergenic
GGATGATTATAAAAGGGGCT+TGG	0.468279	1:-19368566	None:intergenic
AAGGAAGAATCCACTCCGTT+TGG	0.472351	1:+19368228	MsaT001388.1:CDS
TGGCAGACCTTACAATTTCA+CGG	0.479873	1:+19368248	MsaT001388.1:CDS
GGTTTATTGGACAGCCTCAC+GGG	0.486386	1:-19368783	None:intergenic
TGTAGAAAATCCATCTGGTC+TGG	0.486514	1:+19369766	MsaT001388.1:intron
TAATAAGATAGACACTCTTA+AGG	0.488014	1:+19372283	MsaT001388.1:CDS
GCTTAGCAAGCGTTTGTGCT+AGG	0.489007	1:-19372319	None:intergenic
GTTAAAATGATGGACCTCAA+TGG	0.494777	1:-19372381	None:intergenic
AACAGGCTTATTACAATCCT+GGG	0.495397	1:+19368537	MsaT001388.1:intron
ATGTACTGATTATACAAGTA+AGG	0.501884	1:-19368602	None:intergenic
TGAGGCACCGTGAAATTGTA+AGG	0.513205	1:-19368255	None:intergenic
CAACCTGTAAGTTTAATGAC+TGG	0.518291	1:+19369160	MsaT001388.1:CDS
CGGTTTATTGGACAGCCTCA+CGG	0.518948	1:-19368784	None:intergenic
AACCTGTAAGTTTAATGACT+GGG	0.521729	1:+19369161	MsaT001388.1:CDS
AAATTCTGAGCCAGACCAGA+TGG	0.553977	1:-19369776	None:intergenic
ACATTGGACTACAGCTATGC+AGG	0.556528	1:+19368416	MsaT001388.1:CDS
GAACAAATTGTACCTCATTG+AGG	0.556643	1:-19369239	None:intergenic
TGGAAGCAAGAGATCACCAG+AGG	0.574208	1:+19369337	MsaT001388.1:CDS
GTAGTCCAATGTGGTAGCAT+AGG	0.575346	1:-19368405	None:intergenic
TCTAACATGATTACTGCGGA+TGG	0.576063	1:+19369599	MsaT001388.1:CDS
AAATTGTAAGGTCTGCCAAA+CGG	0.577709	1:-19368243	None:intergenic
AAGTAAGGAGCAGCTGCAAG+TGG	0.592524	1:-19368587	None:intergenic
TTTCTCGATCTTGATGGGGA+AGG	0.594200	1:+19368209	None:intergenic
CAAGTGGATGATTATAAAAG+GGG	0.603697	1:-19368571	None:intergenic
AACGCTTGCTAAGCTTTCTG+AGG	0.605311	1:+19372328	MsaT001388.1:CDS
CACCACAAAGAGTTTCAAGT+AGG	0.611543	1:-19374321	None:intergenic
CAATGAAAATGATTCCATTG+AGG	0.612331	1:+19372367	MsaT001388.1:CDS
TAAACCGCACGTAGTTGTTG+AGG	0.614506	1:+19368800	MsaT001388.1:CDS
GGAAGCAAGAGATCACCAGA+GGG	0.616601	1:+19369338	MsaT001388.1:CDS
ACAAACCTATGCTACCACAT+TGG	0.619232	1:+19368400	MsaT001388.1:CDS
GAATTTAGGGACAGTCGTCA+AGG	0.625189	1:+19369793	MsaT001388.1:CDS
ACCTGTAAGTTTAATGACTG+GGG	0.644193	1:+19369162	MsaT001388.1:CDS
AAGGGGCTTGGAGTAGACCC+AGG	0.646981	1:-19368554	None:intergenic
CTCACCTCAACAACTACGTG+CGG	0.655947	1:-19368804	None:intergenic
GTAAGGTCTGCCAAACGGAG+TGG	0.658704	1:-19368238	None:intergenic
GATTTACTGACATCATCTTG+AGG	0.660566	1:-19368273	None:intergenic
TGTGTGCGCAGTGCAGCAAG+TGG	0.664216	1:+19369317	MsaT001388.1:intron
AGCATCAACATCAACAACGT+CGG	0.668557	1:-19369825	None:intergenic
TAAATCTAACATGATTACTG+CGG	0.677606	1:+19369595	MsaT001388.1:CDS
ACAGGGATGGAACCTCAATG+AGG	0.714168	1:+19369227	MsaT001388.1:CDS
TGCATAGCTGTAGTCCAATG+TGG	0.721179	1:-19368414	None:intergenic
ATGCTCTACATGATCCCGTG+AGG	0.726397	1:+19368769	MsaT001388.1:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content

Chromosome	Type	Strat	End	Strand	Name
Chr1	gene	19368222	19374329	19368222	ID=MsaG001388
Chr1	mRNA	19368222	19374329	19368222	ID=MsaT001388.1;Parent=MsaG001388
Chr1	exon	19368222	19368284	19368222	ID=MsaT001388.1.exon1;Parent=MsaT001388.1
Chr1	CDS	19368222	19368284	19368222	ID=cds.MsaT001388.1;Parent=MsaT001388.1
Chr1	exon	19368390	19368437	19368390	ID=MsaT001388.1.exon2;Parent=MsaT001388.1
Chr1	CDS	19368390	19368437	19368390	ID=cds.MsaT001388.1;Parent=MsaT001388.1
Chr1	exon	19368542	19368637	19368542	ID=MsaT001388.1.exon3;Parent=MsaT001388.1
Chr1	CDS	19368542	19368637	19368542	ID=cds.MsaT001388.1;Parent=MsaT001388.1
Chr1	exon	19368744	19368821	19368744	ID=MsaT001388.1.exon4;Parent=MsaT001388.1
Chr1	CDS	19368744	19368821	19368744	ID=cds.MsaT001388.1;Parent=MsaT001388.1
Chr1	exon	19369076	19369248	19369076	ID=MsaT001388.1.exon5;Parent=MsaT001388.1
Chr1	CDS	19369076	19369248	19369076	ID=cds.MsaT001388.1;Parent=MsaT001388.1
Chr1	exon	19369328	19369382	19369328	ID=MsaT001388.1.exon6;Parent=MsaT001388.1
Chr1	CDS	19369328	19369382	19369328	ID=cds.MsaT001388.1;Parent=MsaT001388.1
Chr1	exon	19369572	19369620	19369572	ID=MsaT001388.1.exon7;Parent=MsaT001388.1
Chr1	CDS	19369572	19369620	19369572	ID=cds.MsaT001388.1;Parent=MsaT001388.1
Chr1	exon	19369771	19369862	19369771	ID=MsaT001388.1.exon8;Parent=MsaT001388.1
Chr1	CDS	19369771	19369862	19369771	ID=cds.MsaT001388.1;Parent=MsaT001388.1
Chr1	exon	19371130	19371207	19371130	ID=MsaT001388.1.exon9;Parent=MsaT001388.1
Chr1	CDS	19371130	19371207	19371130	ID=cds.MsaT001388.1;Parent=MsaT001388.1
Chr1	exon	19372263	19372388	19372263	ID=MsaT001388.1.exon10;Parent=MsaT001388.1
Chr1	CDS	19372263	19372388	19372263	ID=cds.MsaT001388.1;Parent=MsaT001388.1
Chr1	exon	19374264	19374329	19374264	ID=MsaT001388.1.exon11;Parent=MsaT001388.1
Chr1	CDS	19374264	19374329	19374264	ID=cds.MsaT001388.1;Parent=MsaT001388.1

Gene Sequence

>MsaT001388.1

ATGGGGAAGGAAGAATCCACTCCGTTTGGCAGACCTTACAATTTCACGGTGCCTCAAGATGATCCAACTCCAAACAAACCTATGCTACCACATTGGACTACAGCTATGCAGGCTTATTACAATCCTGGGTCTACTCCAAGCCCCTTTTATAATCATCCACTTGCAGCTGCTCCTTACTTGTATAATCAGTACATCTTGCAAAATCAGGCAAACAATGCACTACTTCAACGTGATGCTCTACATGATCCCGTGAGGCTGTCCAATAAACCGCACGTAGTTGTTGAGGATGCTGCAAAGGCTTTTTCAGAAATGGTACACAGAAATTTTGGCCAAAAAAACCAAGATTTCAACTTATTTGCTAGAAACTCACAACCTGTAAGTTTAATGACTGGGGAATCAAGAGAATTTGGAAACCAGTCTTCTGCACCCAAAAATGACAGGGATGGAACCTCAATGAGTGCAGCAAGTGGAAGCAAGAGATCACCAGAGGGAGATCAAAATGACAGTAACAAAGATTCTCCTTTAACTAAGAAGCATAAATCTAACATGATTACTGCGGATGAAAATCCATCTGGTCTGGCTCAGAATTTAGGGACAGTCGTCAAGGAATCTGATGCCGACGTTGTTGATGTTGATGCTCAACTTAAAAATACGGAAGGTGATGACATAAGAAGAGAAAGAAAAAGATTGTCAAACAGGAAATCAGCTAAAAGATCAAAGATAAAGAAACAGCAAGAATGTGAAGAGCTATATAATAAGATAGACACTCTTAAGGATGAAAATTCTGTCCTAGCACAAACGCTTGCTAAGCTTTCTGAGGAATGTCTGGAGCTCGCCAATGAAAATGATTCCATTGAGGAAGAGCTAGTTAAGAAGTATGGACCAGAATCAATTGCAGATCTATTGCTCATGAAGCCTACTTGA

Protein sequence

>MsaT001388.1

MGKEESTPFGRPYNFTVPQDDPTPNKPMLPHWTTAMQAYYNPGSTPSPFYNHPLAAAPYLYNQYILQNQANNALLQRDALHDPVRLSNKPHVVVEDAAKAFSEMVHRNFGQKNQDFNLFARNSQPVSLMTGESREFGNQSSAPKNDRDGTSMSAASGSKRSPEGDQNDSNKDSPLTKKHKSNMITADENPSGLAQNLGTVVKESDADVVDVDAQLKNTEGDDIRRERKRLSNRKSAKRSKIKKQQECEELYNKIDTLKDENSVLAQTLAKLSEECLELANENDSIEEELVKKYGPESIADLLLMKPT*