AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG001421

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG001421 MtrunA17_Chr1g0163011 98.128 748 11 2 1 748 1 745 0.0 1511
MsaG001421 MtrunA17_Chr4g0066741 36.667 540 257 14 189 722 179 639 3.52e-88 300
MsaG001421 MtrunA17_Chr5g0403921 33.560 587 285 16 178 733 155 667 1.82e-87 291
MsaG001421 MtrunA17_Chr5g0394201 36.330 545 244 15 198 723 363 823 5.83e-85 290
MsaG001421 MtrunA17_Chr1g0205891 36.746 547 240 15 198 723 376 837 7.79e-84 288
MsaG001421 MtrunA17_Chr2g0286291 36.757 555 258 17 173 722 176 642 4.54e-81 280
MsaG001421 MtrunA17_Chr5g0442141 34.791 549 276 12 198 730 617 1099 4.94e-81 283
MsaG001421 MtrunA17_Chr3g0137091 35.401 548 254 13 198 721 289 760 6.87e-79 276
MsaG001421 MtrunA17_Chr3g0095091 34.657 554 270 13 198 735 571 1048 4.16e-78 275
MsaG001421 MtrunA17_Chr4g0052341 35.874 538 251 14 198 717 745 1206 4.38e-78 275
MsaG001421 MtrunA17_Chr4g0066651 34.991 543 250 15 198 716 989 1452 1.34e-74 264
MsaG001421 MtrunA17_Chr1g0183681 31.560 564 260 16 191 707 385 869 3.55e-70 250
MsaG001421 MtrunA17_Chr7g0232121 30.017 593 305 13 166 720 4 524 3.48e-69 244
MsaG001421 MtrunA17_Chr8g0348721 33.397 524 245 14 198 693 987 1434 3.31e-63 230
MsaG001421 MtrunA17_Chr7g0248031 33.142 522 250 12 198 693 994 1442 1.80e-62 228
MsaG001421 MtrunA17_Chr8g0384531 31.359 574 317 19 197 742 383 907 5.03e-59 216
MsaG001421 MtrunA17_Chr4g0017211 30.245 572 309 19 192 723 1444 1965 4.58e-57 211
MsaG001421 MtrunA17_Chr1g0175191 38.050 318 159 9 198 505 521 810 5.94e-56 208
MsaG001421 MtrunA17_Chr1g0175191 54.305 151 69 0 573 723 1062 1212 4.41e-45 175
MsaG001421 MtrunA17_Chr5g0393461 30.991 555 302 17 197 720 183 687 4.91e-55 204
MsaG001421 MtrunA17_Chr7g0257101 37.920 327 172 9 194 507 574 882 6.14e-55 205
MsaG001421 MtrunA17_Chr7g0257101 48.667 150 77 0 572 721 1191 1340 2.42e-34 141
MsaG001421 MtrunA17_Chr2g0320751 25.880 568 324 16 227 725 463 1002 3.78e-41 162
MsaG001421 MtrunA17_Chr7g0255821 25.222 563 333 18 219 713 250 792 7.04e-35 142
MsaG001421 MtrunA17_Chr3g0086111 24.324 592 363 17 146 708 134 669 2.77e-33 138
MsaG001421 MtrunA17_Chr7g0231521 32.218 239 144 5 166 395 4 233 9.59e-32 132
MsaG001421 MtrunA17_Chr7g0231521 40.000 190 101 2 569 745 282 471 7.60e-31 129
MsaG001421 MtrunA17_Chr7g0233021 25.868 576 313 22 193 707 650 1172 1.65e-28 122
MsaG001421 MtrunA17_Chr4g0040301 46.154 156 81 2 573 726 380 534 5.32e-28 120
MsaG001421 MtrunA17_Chr4g0040301 27.907 258 144 9 227 458 18 259 2.75e-20 95.9
MsaG001421 MtrunA17_Chr2g0296421 23.870 553 354 17 178 713 491 993 4.36e-27 118
MsaG001421 MtrunA17_Chr1g0212331 41.985 131 76 0 591 721 887 1017 5.73e-26 114
MsaG001421 MtrunA17_Chr7g0232971 24.869 571 320 20 193 704 715 1235 1.57e-25 113
MsaG001421 MtrunA17_Chr8g0352361 22.472 534 346 17 198 713 361 844 3.18e-25 112
MsaG001421 MtrunA17_Chr4g0047081 24.094 552 331 19 218 731 699 1200 9.77e-25 110
MsaG001421 MtrunA17_Chr2g0281601 39.130 138 84 0 584 721 1143 1280 4.32e-24 108
MsaG001421 MtrunA17_Chr7g0233001 23.353 501 283 17 252 707 17 461 7.50e-24 106
MsaG001421 MtrunA17_Chr1g0168031 37.748 151 93 1 572 721 985 1135 6.20e-23 105
MsaG001421 MtrunA17_Chr1g0168031 27.557 352 177 15 219 521 534 856 1.19e-16 84.7
MsaG001421 MtrunA17_Chr1g0190381 37.572 173 94 2 567 725 182 354 8.74e-23 103
MsaG001421 MtrunA17_Chr4g0024921 35.417 144 91 1 572 713 763 906 3.77e-21 99.0
MsaG001421 MtrunA17_Chr4g0069451 36.620 142 90 0 573 714 470 611 7.52e-20 94.4
MsaG001421 MtrunA17_Chr4g0069451 30.224 268 147 11 192 440 168 414 6.55e-18 88.2
MsaG001421 MtrunA17_Chr1g0149871 50.000 82 38 2 198 279 244 322 5.32e-17 83.2
MsaG001421 MtrunA17_Chr1g0190391 29.954 217 124 8 198 393 582 791 3.52e-15 79.7
MsaG001421 MtrunA17_Chr2g0320781 32.540 126 73 3 591 707 111 233 1.41e-14 74.3
MsaG001421 MtrunA17_Chr5g0428401 30.921 152 94 2 573 713 1475 1626 1.74e-14 77.8
MsaG001421 MtrunA17_Chr7g0232991 33.571 140 88 2 573 707 461 600 3.62e-14 76.3
MsaG001421 MtrunA17_Chr7g0233031 32.857 140 77 4 573 701 1046 1179 1.18e-13 75.1
MsaG001421 MtrunA17_Chr7g0225791 31.429 140 90 2 574 707 1087 1226 6.33e-12 69.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG001421 AT2G02090.1 70.399 777 184 7 1 746 1 762 0.0 1106
MsaG001421 AT5G19310.2 35.807 539 255 12 198 723 122 582 4.02e-86 289
MsaG001421 AT5G19310.1 35.807 539 255 12 198 723 386 846 3.68e-84 289
MsaG001421 AT3G06010.1 35.490 541 255 11 198 723 403 864 5.74e-83 286
MsaG001421 AT2G13370.1 36.106 565 272 15 198 745 625 1117 1.06e-81 285
MsaG001421 AT2G13370.2 36.106 565 272 15 198 745 625 1117 1.06e-81 285
MsaG001421 AT2G13370.3 36.106 565 272 15 198 745 625 1117 1.06e-81 285
MsaG001421 AT2G25170.1 33.638 547 267 13 197 721 272 744 1.32e-78 276
MsaG001421 AT2G25170.4 33.638 547 267 13 197 721 272 744 1.32e-78 276
MsaG001421 AT2G25170.2 33.638 547 267 13 197 721 289 761 1.33e-78 276
MsaG001421 AT2G25170.3 33.638 547 267 13 197 721 298 770 2.15e-78 275
MsaG001421 AT4G31900.2 34.280 528 264 13 198 721 184 632 1.74e-77 271
MsaG001421 AT4G31900.3 34.280 528 264 13 198 721 225 673 2.96e-77 271
MsaG001421 AT4G31900.1 34.280 528 264 13 198 721 225 673 4.12e-77 270
MsaG001421 AT5G44800.1 34.783 529 263 11 201 717 692 1150 2.94e-75 266
MsaG001421 AT2G28290.6 33.702 543 257 15 198 716 755 1218 1.15e-72 259
MsaG001421 AT2G28290.2 33.702 543 257 15 198 716 754 1217 1.18e-72 259
MsaG001421 AT2G28290.3 33.702 543 257 15 198 716 754 1217 1.19e-72 259
MsaG001421 AT2G28290.4 33.702 543 257 15 198 716 754 1217 1.19e-72 259
MsaG001421 AT2G28290.1 33.702 543 257 15 198 716 754 1217 1.46e-72 259
MsaG001421 AT2G28290.5 33.702 543 257 15 198 716 755 1218 1.46e-72 259
MsaG001421 AT2G18760.4 30.686 554 257 13 201 707 388 861 4.30e-69 247
MsaG001421 AT2G18760.1 30.686 554 257 13 201 707 388 861 4.30e-69 247
MsaG001421 AT2G18760.2 30.686 554 257 13 201 707 388 861 4.30e-69 247
MsaG001421 AT2G18760.7 30.686 554 257 13 201 707 388 861 4.30e-69 247
MsaG001421 AT2G18760.5 30.686 554 257 13 201 707 388 861 4.30e-69 247
MsaG001421 AT2G18760.6 30.686 554 257 13 201 707 388 861 4.30e-69 247
MsaG001421 AT2G18760.3 30.686 554 257 13 201 707 447 920 5.31e-69 247
MsaG001421 AT2G44980.1 32.264 561 286 14 198 738 51 537 6.63e-69 243
MsaG001421 AT2G44980.3 31.379 580 284 15 198 738 51 555 1.52e-66 237
MsaG001421 AT2G44980.2 31.034 580 287 14 198 738 51 556 6.83e-66 235
MsaG001421 AT2G46020.2 33.973 521 247 12 198 693 981 1429 6.03e-64 233
MsaG001421 AT2G46020.3 33.973 521 247 12 198 693 981 1429 6.03e-64 233
MsaG001421 AT2G46020.4 33.973 521 247 12 198 693 981 1429 6.03e-64 233
MsaG001421 AT2G46020.5 34.170 518 249 11 198 693 981 1428 3.18e-63 231
MsaG001421 AT2G46020.1 34.170 518 249 11 198 693 981 1428 3.18e-63 231
MsaG001421 AT2G46020.6 34.170 518 249 11 198 693 981 1428 3.18e-63 231
MsaG001421 AT1G03750.1 30.969 578 308 16 201 725 142 681 7.43e-61 220
MsaG001421 AT5G63950.1 31.004 558 313 17 197 728 376 887 1.47e-59 219
MsaG001421 AT3G54280.1 30.944 572 294 21 198 723 1451 1967 5.42e-58 215
MsaG001421 AT3G54280.2 30.944 572 294 21 198 723 1482 1998 6.75e-58 214
MsaG001421 AT5G63950.2 30.380 553 306 16 197 728 376 870 2.48e-57 212
MsaG001421 AT3G19210.3 30.325 554 307 16 197 720 179 683 5.69e-55 202
MsaG001421 AT3G19210.1 30.325 554 307 16 197 720 179 683 9.91e-55 203
MsaG001421 AT3G12810.2 37.383 321 157 10 198 505 532 821 3.92e-54 203
MsaG001421 AT3G12810.2 52.288 153 73 0 573 725 1071 1223 2.73e-43 170
MsaG001421 AT3G12810.1 37.383 321 157 10 198 505 536 825 3.95e-54 203
MsaG001421 AT3G12810.1 52.288 153 73 0 573 725 1075 1227 2.80e-43 170
MsaG001421 AT3G19210.2 29.964 554 307 16 197 720 179 681 2.81e-53 199
MsaG001421 AT3G57300.1 35.780 327 179 9 194 507 582 890 9.66e-51 192
MsaG001421 AT3G57300.1 48.000 150 78 0 572 721 1205 1354 2.72e-34 142
MsaG001421 AT3G57300.4 35.780 327 179 9 194 507 582 890 9.66e-51 192
MsaG001421 AT3G57300.4 48.000 150 78 0 572 721 1205 1354 2.72e-34 142
MsaG001421 AT3G57300.3 35.780 327 179 9 194 507 582 890 1.69e-50 192
MsaG001421 AT5G18620.2 34.393 346 173 11 170 506 176 476 2.68e-48 184
MsaG001421 AT5G18620.2 51.128 133 64 1 591 722 513 645 1.18e-30 130
MsaG001421 AT5G18620.1 34.393 346 173 11 170 506 176 476 3.44e-48 184
MsaG001421 AT5G18620.1 51.128 133 64 1 591 722 513 645 1.20e-30 130
MsaG001421 AT3G06400.2 34.024 338 181 11 172 506 173 471 1.11e-47 182
MsaG001421 AT3G06400.2 51.880 133 63 1 591 722 508 640 2.21e-31 132
MsaG001421 AT3G06400.3 34.024 338 181 11 172 506 173 471 1.18e-47 182
MsaG001421 AT3G06400.3 51.880 133 63 1 591 722 508 640 2.31e-31 132
MsaG001421 AT3G06400.1 34.024 338 181 11 172 506 173 471 1.42e-47 182
MsaG001421 AT3G06400.1 51.880 133 63 1 591 722 508 640 2.48e-31 132
MsaG001421 AT3G57300.2 32.500 360 179 10 194 507 582 923 5.39e-47 181
MsaG001421 AT3G57300.2 48.000 150 78 0 572 721 1238 1387 3.15e-34 141
MsaG001421 AT5G22750.1 26.198 626 319 16 219 725 414 1015 2.10e-45 176
MsaG001421 AT5G66750.1 33.634 333 167 12 198 506 202 504 6.01e-45 173
MsaG001421 AT5G66750.1 50.365 137 63 2 588 721 539 673 5.69e-33 136
MsaG001421 AT5G43530.1 28.250 623 313 24 219 737 682 1274 1.92e-40 160
MsaG001421 AT1G02670.7 27.036 577 328 17 196 726 122 651 1.01e-38 153
MsaG001421 AT1G02670.1 27.036 577 328 17 196 726 133 662 1.52e-38 153
MsaG001421 AT1G48310.2 27.405 551 287 17 192 725 171 625 3.26e-38 152
MsaG001421 AT1G08600.4 28.257 591 327 19 198 722 719 1278 1.62e-37 152
MsaG001421 AT1G08600.3 28.257 591 327 19 198 722 719 1278 1.62e-37 152
MsaG001421 AT1G08600.2 28.257 591 327 19 198 722 719 1278 1.62e-37 152
MsaG001421 AT1G08600.1 28.179 582 318 19 198 722 719 1257 1.93e-37 151
MsaG001421 AT1G48310.1 26.134 551 294 15 192 725 166 620 2.53e-36 146
MsaG001421 AT1G48310.3 26.296 540 285 15 192 714 166 609 4.44e-36 145
MsaG001421 AT5G07810.1 23.006 539 330 15 198 714 204 679 3.67e-29 125
MsaG001421 AT2G21450.2 23.704 540 349 15 198 713 272 772 3.64e-26 115
MsaG001421 AT2G21450.1 23.704 540 349 15 198 713 265 765 5.06e-26 115
MsaG001421 AT2G21450.3 23.704 540 349 15 198 713 174 674 8.03e-26 114
MsaG001421 AT1G61140.3 38.686 137 84 0 585 721 961 1097 3.19e-24 109
MsaG001421 AT1G61140.1 38.686 137 84 0 585 721 1119 1255 4.11e-24 109
MsaG001421 AT2G16390.2 25.092 546 305 24 198 704 353 833 4.85e-24 108
MsaG001421 AT2G16390.1 25.092 546 305 24 198 704 350 830 6.05e-24 108
MsaG001421 AT1G05120.2 37.278 169 101 3 572 737 704 870 1.24e-23 107
MsaG001421 AT1G11100.5 36.957 138 87 0 588 725 927 1064 1.25e-23 107
MsaG001421 AT1G11100.5 24.128 344 175 12 219 502 422 739 1.39e-13 75.1
MsaG001421 AT1G05120.1 37.278 169 101 3 572 737 661 827 1.26e-23 107
MsaG001421 AT1G11100.3 36.957 138 87 0 588 725 971 1108 1.35e-23 107
MsaG001421 AT1G11100.2 36.957 138 87 0 588 725 1113 1250 1.65e-23 107
MsaG001421 AT1G11100.1 36.957 138 87 0 588 725 1070 1207 1.77e-23 107
MsaG001421 AT1G11100.1 24.128 344 175 12 219 502 565 882 8.50e-14 75.9
MsaG001421 AT1G11100.4 36.957 138 87 0 588 725 1114 1251 1.79e-23 107
MsaG001421 AT3G20010.2 38.931 131 80 0 591 721 603 733 2.23e-23 106
MsaG001421 AT3G20010.3 38.931 131 80 0 591 721 628 758 2.25e-23 106
MsaG001421 AT1G50410.2 38.931 131 80 0 591 721 828 958 2.96e-23 106
MsaG001421 AT1G50410.1 38.931 131 80 0 591 721 828 958 2.96e-23 106
MsaG001421 AT1G50410.3 38.931 131 80 0 591 721 828 958 2.96e-23 106
MsaG001421 AT3G20010.1 38.931 131 80 0 591 721 894 1024 4.17e-23 105
MsaG001421 AT3G20010.5 38.931 131 80 0 591 721 894 1024 4.17e-23 105
MsaG001421 AT3G20010.7 38.931 131 80 0 591 721 894 1024 4.17e-23 105
MsaG001421 AT1G02670.6 38.971 136 83 0 591 726 512 647 3.36e-22 102
MsaG001421 AT1G02670.6 30.846 201 117 6 196 386 95 283 2.10e-16 84.0
MsaG001421 AT1G02670.3 38.971 136 83 0 591 726 539 674 3.95e-22 102
MsaG001421 AT1G02670.3 30.846 201 117 6 196 386 122 310 3.32e-16 83.2
MsaG001421 AT5G05130.1 37.500 128 78 1 591 716 727 854 1.78e-19 94.0
MsaG001421 AT5G05130.1 25.820 244 160 9 252 488 361 590 3.68e-14 77.0
MsaG001421 AT1G02670.5 41.346 104 61 0 591 694 512 615 6.54e-18 88.6
MsaG001421 AT1G02670.5 30.846 201 117 6 196 386 95 283 3.46e-16 83.2
MsaG001421 AT1G02670.2 41.346 104 61 0 591 694 539 642 7.63e-18 88.6
MsaG001421 AT1G02670.2 30.846 201 117 6 196 386 122 310 5.23e-16 82.8
MsaG001421 AT1G48310.4 26.101 318 197 11 192 492 166 462 3.44e-17 85.9
MsaG001421 AT3G16600.1 29.478 268 141 10 196 426 52 308 1.41e-16 84.3
MsaG001421 AT3G16600.1 41.509 106 57 2 620 721 483 587 1.31e-13 75.1
MsaG001421 AT1G02670.4 30.846 201 117 6 196 386 122 310 2.19e-16 83.6
MsaG001421 AT2G40770.2 34.307 137 73 2 591 713 1451 1584 1.45e-15 82.0
MsaG001421 AT2G40770.1 34.307 137 73 2 591 713 1451 1584 1.45e-15 82.0
MsaG001421 AT2G40770.3 34.307 137 73 2 591 713 1451 1584 1.45e-15 82.0
MsaG001421 AT1G05490.1 33.813 139 80 3 572 701 1198 1333 7.42e-14 76.3
MsaG001421 AT1G05490.1 26.860 242 135 7 218 424 857 1091 7.11e-12 69.7
MsaG001421 AT3G24340.1 31.724 145 87 3 591 726 945 1086 3.86e-12 70.5

Find 175 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGTCGTATGAAGTCTATATT+AGG 0.202585 1:-19864851 MsaT001421.1:CDS
GGGGAGTTGTTTGGTTCTTC+TGG 0.212962 1:-19868591 MsaT001421.1:CDS
GACATCAATGCTTGATATAT+TGG 0.245544 1:-19861854 MsaT001421.1:CDS
AGTTTGTCCTGCTTCTGTTT+TGG 0.261009 1:-19867379 MsaT001421.1:CDS
CTACATCCAATAGGTGCTTT+TGG 0.267000 1:-19863885 MsaT001421.1:CDS
GCTGCATCACATCAGATTTC+AGG 0.290922 1:+19864810 None:intergenic
AATCACTTGCACAACGATTC+TGG 0.297609 1:-19867414 MsaT001421.1:CDS
TGATATATTGGAGTGGGCTT+TGG 0.308912 1:-19861842 MsaT001421.1:CDS
CGATAACATCCTTGATGAAT+TGG 0.317239 1:-19868847 MsaT001421.1:CDS
TTCAGGCGCCGTAAAATAAA+TGG 0.317710 1:+19864827 None:intergenic
CTTCTGGTGCGGTTTGTGAT+AGG 0.319349 1:-19868575 MsaT001421.1:CDS
CCCTTCTTTGATCTCTTCCA+TGG 0.334281 1:+19859346 None:intergenic
AAATACCTGTATACGGTTAC+AGG 0.336163 1:+19859565 None:intergenic
GCAGAAGTGTGCGAGGATTT+CGG 0.339211 1:-19868625 MsaT001421.1:CDS
GAGTGGGCTTTGGATGTCAT+TGG 0.340472 1:-19861832 MsaT001421.1:CDS
CAGACGAGAAGAACATTAAA+TGG 0.340627 1:+19867234 None:intergenic
GCCCATTTCATCCGCCAATA+TGG 0.343241 1:+19867570 None:intergenic
CGAACCATCCCTTGTTGATA+AGG 0.343422 1:-19863950 MsaT001421.1:CDS
AGCCATATTGGCGGATGAAA+TGG 0.345809 1:-19867572 MsaT001421.1:CDS
CCTTCCATCAAGCCGTTTGT+AGG 0.346388 1:+19861804 None:intergenic
GGACAAGGCTTAAACTTAAC+TGG 0.347977 1:-19859679 MsaT001421.1:CDS
TGCGTGTGGTTCCGAGGATT+CGG 0.374934 1:-19868393 MsaT001421.1:CDS
TGAATTTGTTACAGGAGTTA+TGG 0.377752 1:-19864964 MsaT001421.1:intron
AGATGCAAATGAAGATGATT+TGG 0.389584 1:-19868661 MsaT001421.1:CDS
TAGGATCCCCTTTCTATCAT+TGG 0.390282 1:+19862080 None:intergenic
GGAGGATGAGGAGGTGGTTG+CGG 0.390551 1:+19868927 None:intergenic
GGGCCTTGGCAAAACGGTTC+AGG 0.392499 1:-19867551 MsaT001421.1:intron
GCATAACATGTTTATCTGAT+AGG 0.397941 1:+19862061 None:intergenic
AGCTCCACTTCCAACGCTTC+AGG 0.400293 1:+19867076 None:intergenic
AGTGGGCTTTGGATGTCATT+GGG 0.405126 1:-19861831 MsaT001421.1:CDS
TTGAATTCCTTGTCCAAGTC+AGG 0.408175 1:-19867267 MsaT001421.1:CDS
ATCTTCATTTGCATCTTCAA+TGG 0.410370 1:+19868668 None:intergenic
TGCGGTTTGTGATAGGTATT+CGG 0.411479 1:-19868568 MsaT001421.1:CDS
TCAAAGCCAAAAGCACCTAT+TGG 0.413493 1:+19863879 None:intergenic
TTAAAGCCGTATCAGCTTGT+TGG 0.415563 1:-19868356 MsaT001421.1:CDS
CAATTCATCAAGGATGTTAT+CGG 0.424378 1:+19868848 None:intergenic
TTCGGAAGTTGAATCTTCTT+CGG 0.429991 1:-19868550 MsaT001421.1:CDS
GAAGAGGATGCTGGAGGTAG+TGG 0.431204 1:-19868741 MsaT001421.1:CDS
CATGTGTCATACAGCCATAT+TGG 0.431262 1:-19867584 MsaT001421.1:intron
GAAGAAGGAGGATGAGGAGG+TGG 0.436478 1:+19868921 None:intergenic
GCTGCTCCGACGGCGAGACC+TGG 0.440982 1:-19868792 MsaT001421.1:CDS
AATGAGCAAATACCTGTATA+CGG 0.441963 1:+19859558 None:intergenic
ATCTCTCCCATCGTCTTCTC+AGG 0.444051 1:+19859317 None:intergenic
TTGACCTACAAACGGCTTGA+TGG 0.446547 1:-19861808 MsaT001421.1:CDS
TACTTTGGACAGAGTTATAG+AGG 0.446614 1:-19863853 MsaT001421.1:CDS
GGCGGCTGCTGCTGCTCCGA+CGG 0.448279 1:-19868802 MsaT001421.1:CDS
ACAGAAGAAGAAGGAGGATG+AGG 0.448978 1:+19868915 None:intergenic
ACGAAATCTCCGACGACGAA+TGG 0.451974 1:-19869034 MsaT001421.1:CDS
GAAGATCGTTGCCATCGAAT+TGG 0.455443 1:-19859598 MsaT001421.1:CDS
GCCATATTGGCGGATGAAAT+GGG 0.457744 1:-19867571 MsaT001421.1:CDS
GTGGATAATGTAGGCTTTAG+CGG 0.460351 1:-19861932 MsaT001421.1:intron
TGCTGTTGTTGTATCGGAAG+AGG 0.460864 1:-19868322 MsaT001421.1:CDS
GCAATCGTCGTCGTCGGAAA+CGG 0.463848 1:+19868890 None:intergenic
GCTAGGAAACTACATCCAAT+AGG 0.464188 1:-19863894 MsaT001421.1:CDS
GAGTTGAAAGGGGAGTTGTT+TGG 0.464548 1:-19868600 MsaT001421.1:CDS
ATCGTAAAATCCTGAAGCGT+TGG 0.466630 1:-19867086 MsaT001421.1:CDS
AACCAACGCTTGATGCTGAC+AGG 0.467572 1:-19866970 MsaT001421.1:CDS
TGTCGTAATGGAAAAGCAAC+AGG 0.468244 1:-19864232 MsaT001421.1:CDS
GATGAGGATGAAGAGGATGC+TGG 0.469429 1:-19868750 MsaT001421.1:CDS
TGCTGAATGTGATGCGGCGG+CGG 0.472132 1:-19868820 MsaT001421.1:CDS
GTCGTATGAAGTCTATATTA+GGG 0.474577 1:-19864850 MsaT001421.1:CDS
CAAAGCCTGTAACCGTATAC+AGG 0.476498 1:-19859570 MsaT001421.1:intron
CGAAATCTCCGACGACGAAT+GGG 0.477170 1:-19869033 MsaT001421.1:CDS
TGCTGGAGGTAGTGGTGGTG+TGG 0.478844 1:-19868733 MsaT001421.1:CDS
GGATTTCGGTGGAGTTGAAA+GGG 0.480790 1:-19868611 MsaT001421.1:CDS
TTGGCGGATGAAATGGGCCT+TGG 0.480876 1:-19867565 MsaT001421.1:CDS
CTGCTTGTCTATCAATCTGT+GGG 0.483659 1:+19859620 None:intergenic
TGCTTGCTTTCAACTAGAGC+TGG 0.483853 1:-19859703 MsaT001421.1:CDS
TTATGGTACCAATGATAGAA+AGG 0.487592 1:-19862088 MsaT001421.1:CDS
AAAGATTCGAGAGGCGGAGG+AGG 0.490488 1:+19868963 None:intergenic
TATGGTACCAATGATAGAAA+GGG 0.493526 1:-19862087 MsaT001421.1:CDS
TGAAATGGGCCTTGGCAAAA+CGG 0.495673 1:-19867557 MsaT001421.1:CDS
TCTGCTTGTCTATCAATCTG+TGG 0.496313 1:+19859619 None:intergenic
GTCGGAGATTTCGTATAACT+CGG 0.497644 1:+19869043 None:intergenic
TCCATGGAAGAGATCAAAGA+AGG 0.498108 1:-19859347 MsaT001421.1:CDS
TATGCTTTCTGCGAAATGTC+GGG 0.499381 1:-19862043 MsaT001421.1:intron
GTAGAAGATTACCCGGTGAA+TGG 0.500798 1:+19863806 None:intergenic
TTCAACTGAAGATGAAATTG+AGG 0.501071 1:-19868694 MsaT001421.1:CDS
AAACTTAACTGGAGCTGATA+CGG 0.502508 1:-19859668 MsaT001421.1:CDS
TTTCTGTCCTTCAATATCAC+GGG 0.502860 1:-19867317 MsaT001421.1:CDS
CACTGCAGGCTGGTGACAAA+GGG 0.505063 1:-19859437 MsaT001421.1:intron
GAAGTGGAGCTGTGTGCTGA+TGG 0.505582 1:-19867064 MsaT001421.1:CDS
GGATGAGGAGGTGGTTGCGG+TGG 0.506482 1:+19868930 None:intergenic
AGGATTTCGGTGGAGTTGAA+AGG 0.507085 1:-19868612 MsaT001421.1:CDS
GTGCAGACCCGTGATATTGA+AGG 0.508019 1:+19867310 None:intergenic
TGCTGCTGTTCTAGAATCCA+TGG 0.510112 1:-19859363 MsaT001421.1:CDS
TTGTTGTATCGGAAGAGGAT+TGG 0.510251 1:-19868317 MsaT001421.1:CDS
TAGAAAGATTCGAGAGGCGG+AGG 0.510394 1:+19868960 None:intergenic
GAGATTGCAAAGAGAAAGCT+AGG 0.510912 1:-19859392 MsaT001421.1:CDS
TTATGCTTTCTGCGAAATGT+CGG 0.511769 1:-19862044 MsaT001421.1:CDS
AAATCCTGAAGCGTTGGAAG+TGG 0.516959 1:-19867080 MsaT001421.1:CDS
GGATGAGGCACATGCTTTGA+AGG 0.517420 1:-19867043 MsaT001421.1:CDS
TTAACACCAACAAGCTGATA+CGG 0.523745 1:+19868350 None:intergenic
TGGAGGAGGCAATCCTGACT+TGG 0.523980 1:+19867254 None:intergenic
TCATTGGGTTGACCTACAAA+CGG 0.530604 1:-19861816 MsaT001421.1:CDS
CCACTGCAGGCTGGTGACAA+AGG 0.531295 1:-19859438 MsaT001421.1:intron
ATCGTGCTGTTTCACAAGCT+CGG 0.532030 1:-19864179 MsaT001421.1:CDS
GAAGAAGAAGGAGGATGAGG+AGG 0.532701 1:+19868918 None:intergenic
GCAGGTTCAATATGTCGTAA+TGG 0.533569 1:-19864244 MsaT001421.1:intron
TTGTATCGGAAGAGGATTGG+AGG 0.533601 1:-19868314 MsaT001421.1:CDS
TTCGGTTAGAATTGTTACTC+AGG 0.533772 1:-19868532 MsaT001421.1:intron
TCAACTAGAGCTGGTGGACA+AGG 0.534658 1:-19859694 MsaT001421.1:CDS
GCAACAGGAACATGCTTACA+AGG 0.535721 1:-19864217 MsaT001421.1:CDS
ACAGGAGTTATGGTCTATGT+TGG 0.535877 1:-19864954 MsaT001421.1:intron
CCATGGAAGAGATCAAAGAA+GGG 0.538142 1:-19859346 MsaT001421.1:CDS
ACAATGACTTCTCCATTCAC+CGG 0.542504 1:-19863818 MsaT001421.1:CDS
TGACCACTCTTCTTTAGTGA+TGG 0.544085 1:+19861901 None:intergenic
TTCAAAGCAATCGTCGTCGT+CGG 0.547487 1:+19868884 None:intergenic
GTGATATTGAAGGACAGAAA+AGG 0.551063 1:+19867320 None:intergenic
AGGATGTTGTTCGCTTTGCT+AGG 0.551810 1:-19863911 MsaT001421.1:CDS
AGCTGATACGGCTTTAACAA+CGG 0.555410 1:+19868362 None:intergenic
GAGGTTTATTATAGATGATG+AGG 0.556603 1:-19868766 MsaT001421.1:CDS
AATGCTTGATATATTGGAGT+GGG 0.556860 1:-19861848 MsaT001421.1:CDS
TATAGATGATGAGGATGAAG+AGG 0.557091 1:-19868757 MsaT001421.1:CDS
TAATTATTTATTTGCCGTCG+AGG 0.558803 1:+19864108 None:intergenic
TCGGAGATTTCGTATAACTC+GGG 0.560591 1:+19869044 None:intergenic
TTCGCCTTATCAACAAGGGA+TGG 0.563297 1:+19863946 None:intergenic
CAATGACTTCTCCATTCACC+GGG 0.563438 1:-19863817 MsaT001421.1:intron
TGCTTACAAGGAAGCCATTG+AGG 0.570810 1:-19864205 MsaT001421.1:CDS
AGAAGAACATTAAATGGAGG+AGG 0.574338 1:+19867240 None:intergenic
CTCCGTCCAGGTCTCGCCGT+CGG 0.575296 1:+19868786 None:intergenic
GCTTTGTTTGACCAATTCGA+TGG 0.581682 1:+19859587 None:intergenic
CGGTTGAAAATCCGAATCCT+CGG 0.582923 1:+19868382 None:intergenic
TCCCTGTCAGCATCAAGCGT+TGG 0.583066 1:+19866968 None:intergenic
CTCCGACGGCGAGACCTGGA+CGG 0.585948 1:-19868788 MsaT001421.1:CDS
CAGCATCATCCAATTCATCA+AGG 0.587248 1:+19868838 None:intergenic
TCTATAATAAACCTCCGTCC+AGG 0.590421 1:+19868774 None:intergenic
GCAAATGAAGATGATTTGGT+TGG 0.590566 1:-19868657 MsaT001421.1:CDS
GTGATTCTCCCATTCGTCGT+CGG 0.594242 1:+19869025 None:intergenic
CCTACAAACGGCTTGATGGA+AGG 0.595176 1:-19861804 MsaT001421.1:intron
GTGTCATACAGCCATATTGG+CGG 0.595398 1:-19867581 MsaT001421.1:intron
GACATGCCTGAGAAGACGAT+GGG 0.595911 1:-19859323 MsaT001421.1:CDS
ATTGAAGGACAGAAAAGGAC+GGG 0.598964 1:+19867325 None:intergenic
AGAGGCGGAGGAGGAGAACG+AGG 0.600254 1:+19868972 None:intergenic
AACAGCACGATACTCCTCAA+TGG 0.603233 1:+19864191 None:intergenic
GAGGATGCTGGAGGTAGTGG+TGG 0.604373 1:-19868738 MsaT001421.1:CDS
GAGCTGTGTGCTGATGGATG+AGG 0.605324 1:-19867058 MsaT001421.1:CDS
GTTGTTTGGTTCTTCTGGTG+CGG 0.605793 1:-19868586 MsaT001421.1:CDS
TACGGTTGTGATTCATGACA+TGG 0.608110 1:-19859650 MsaT001421.1:CDS
TAATAGAAAGATTCGAGAGG+CGG 0.608602 1:+19868957 None:intergenic
GCAGGACAAACTATAAGATG+TGG 0.609749 1:+19867390 None:intergenic
CCAGCACACAGGTAGCAGAA+AGG 0.612596 1:-19859771 MsaT001421.1:intron
CAATGCTTGATATATTGGAG+TGG 0.614638 1:-19861849 MsaT001421.1:CDS
TAAATTCGCCTTATCAACAA+GGG 0.615776 1:+19863942 None:intergenic
AAGGCGAATTTATAATGATG+AGG 0.615979 1:-19863931 MsaT001421.1:CDS
TGCACGCGCAGCATACTGCA+AGG 0.616432 1:-19867292 MsaT001421.1:CDS
GAAACGGTTACAGAAGAAGA+AGG 0.617325 1:+19868906 None:intergenic
ATAAATTCGCCTTATCAACA+AGG 0.618328 1:+19863941 None:intergenic
TGATGTTGCGTGTGGTTCCG+AGG 0.623054 1:-19868399 MsaT001421.1:CDS
TATCGGAAGAGGATTGGAGG+AGG 0.624019 1:-19868311 MsaT001421.1:intron
GGACATGCCTGAGAAGACGA+TGG 0.627307 1:-19859324 MsaT001421.1:CDS
TTGCTTTCAACTAGAGCTGG+TGG 0.629655 1:-19859700 MsaT001421.1:CDS
AGGTCGTTTAAGAACACGTG+AGG 0.629827 1:+19868992 None:intergenic
AGGCAATCCTGACTTGGACA+AGG 0.633867 1:+19867260 None:intergenic
GATTTCGGTGGAGTTGAAAG+GGG 0.635229 1:-19868610 MsaT001421.1:CDS
ATGGTACCAATGATAGAAAG+GGG 0.637574 1:-19862086 MsaT001421.1:CDS
CTTCCATCACTAAAGAAGAG+TGG 0.641874 1:-19861904 MsaT001421.1:CDS
ACCAACGCTTGATGCTGACA+GGG 0.650956 1:-19866969 MsaT001421.1:CDS
AAGCTTTGCAGAAGTGTGCG+AGG 0.656865 1:-19868632 MsaT001421.1:CDS
GGTCGTTTAAGAACACGTGA+GGG 0.657011 1:+19868993 None:intergenic
CATGGAAGAGATCAAAGAAG+GGG 0.661440 1:-19859345 MsaT001421.1:CDS
GGGAAAGGGAACTAAAGAAG+TGG 0.662059 1:-19867350 MsaT001421.1:CDS
AGGATGTTATCGGTATTTCG+CGG 0.664107 1:+19868858 None:intergenic
ACGAGAAGAACATTAAATGG+AGG 0.665822 1:+19867237 None:intergenic
TGATGCTGAATGTGATGCGG+CGG 0.667482 1:-19868823 MsaT001421.1:CDS
GATGATGTTGATGTTGCGTG+TGG 0.673667 1:-19868407 MsaT001421.1:CDS
TATTGAAGGACAGAAAAGGA+CGG 0.676101 1:+19867324 None:intergenic
GAAGTGTGCGAGGATTTCGG+TGG 0.679153 1:-19868622 MsaT001421.1:CDS
CGACGGCGAGACCTGGACGG+AGG 0.686254 1:-19868785 MsaT001421.1:CDS
CTATTTGACTCCCAGCACAC+AGG 0.689141 1:-19859782 MsaT001421.1:intron
GAGGATGAAGAGGATGCTGG+AGG 0.696952 1:-19868747 MsaT001421.1:CDS
TTATAATAGAAAGATTCGAG+AGG 0.709391 1:+19868954 None:intergenic
GGATGATGCTGAATGTGATG+CGG 0.710544 1:-19868826 MsaT001421.1:CDS
ACGGTTACAGAAGAAGAAGG+AGG 0.715982 1:+19868909 None:intergenic
ATTGACTAAAGATCAAAACA+CGG 0.772497 1:+19861878 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr1 gene 19859289 19869072 19859289 ID=MsaG001421
Chr1 mRNA 19859289 19869072 19859289 ID=MsaT001421.1;Parent=MsaG001421
Chr1 exon 19859289 19859451 19859289 ID=MsaT001421.1.exon12;Parent=MsaT001421.1
Chr1 CDS 19859289 19859451 19859289 ID=cds.MsaT001421.1;Parent=MsaT001421.1
Chr1 exon 19859571 19859789 19859571 ID=MsaT001421.1.exon11;Parent=MsaT001421.1
Chr1 CDS 19859571 19859789 19859571 ID=cds.MsaT001421.1;Parent=MsaT001421.1
Chr1 exon 19861805 19861941 19861805 ID=MsaT001421.1.exon10;Parent=MsaT001421.1
Chr1 CDS 19861805 19861941 19861805 ID=cds.MsaT001421.1;Parent=MsaT001421.1
Chr1 exon 19862044 19862121 19862044 ID=MsaT001421.1.exon9;Parent=MsaT001421.1
Chr1 CDS 19862044 19862121 19862044 ID=cds.MsaT001421.1;Parent=MsaT001421.1
Chr1 exon 19863818 19863976 19863818 ID=MsaT001421.1.exon8;Parent=MsaT001421.1
Chr1 CDS 19863818 19863976 19863818 ID=cds.MsaT001421.1;Parent=MsaT001421.1
Chr1 exon 19864089 19864262 19864089 ID=MsaT001421.1.exon7;Parent=MsaT001421.1
Chr1 CDS 19864089 19864262 19864089 ID=cds.MsaT001421.1;Parent=MsaT001421.1
Chr1 exon 19864787 19864972 19864787 ID=MsaT001421.1.exon6;Parent=MsaT001421.1
Chr1 CDS 19864787 19864972 19864787 ID=cds.MsaT001421.1;Parent=MsaT001421.1
Chr1 exon 19866945 19867125 19866945 ID=MsaT001421.1.exon5;Parent=MsaT001421.1
Chr1 CDS 19866945 19867125 19866945 ID=cds.MsaT001421.1;Parent=MsaT001421.1
Chr1 exon 19867210 19867460 19867210 ID=MsaT001421.1.exon4;Parent=MsaT001421.1
Chr1 CDS 19867210 19867460 19867210 ID=cds.MsaT001421.1;Parent=MsaT001421.1
Chr1 exon 19867552 19867592 19867552 ID=MsaT001421.1.exon3;Parent=MsaT001421.1
Chr1 CDS 19867552 19867592 19867552 ID=cds.MsaT001421.1;Parent=MsaT001421.1
Chr1 exon 19868312 19868429 19868312 ID=MsaT001421.1.exon2;Parent=MsaT001421.1
Chr1 CDS 19868312 19868429 19868312 ID=cds.MsaT001421.1;Parent=MsaT001421.1
Chr1 exon 19868533 19869072 19868533 ID=MsaT001421.1.exon1;Parent=MsaT001421.1
Chr1 CDS 19868533 19869072 19868533 ID=cds.MsaT001421.1;Parent=MsaT001421.1
Gene Sequence

>MsaT001421.1

ATGAAACCCGAGTTATACGAAATCTCCGACGACGAATGGGAGAATCACTCTTTCAAACCCTCACGTGTTCTTAAACGACCTCGTTCTCCTCCTCCGCCTCTCGAATCTTTCTATTATAAACCACCGCAACCACCTCCTCATCCTCCTTCTTCTTCTGTAACCGTTTCCGACGACGACGATTGCTTTGAAATACCGCGAAATACCGATAACATCCTTGATGAATTGGATGATGCTGAATGTGATGCGGCGGCGGCTGCTGCTGCTCCGACGGCGAGACCTGGACGGAGGTTTATTATAGATGATGAGGATGAAGAGGATGCTGGAGGTAGTGGTGGTGTGGATTTGTATGAGATTGATTCAACTGAAGATGAAATTGAGGACGCCATTGAAGATGCAAATGAAGATGATTTGGTTGGTAAAGCTTTGCAGAAGTGTGCGAGGATTTCGGTGGAGTTGAAAGGGGAGTTGTTTGGTTCTTCTGGTGCGGTTTGTGATAGGTATTCGGAAGTTGAATCTTCTTCGGTTAGAATTGTTACTCAGGATGATGTTGATGTTGCGTGTGGTTCCGAGGATTCGGATTTTCAACCGTTGTTAAAGCCGTATCAGCTTGTTGGTGTTAATTTTTTGCTGTTGTTGTATCGGAAGAGGATTGGAGGAGCCATATTGGCGGATGAAATGGGCCTTGGCAAAACGGTTCAGGCAATCACATATTTGACTTTGTTGAATCACTTGCACAACGATTCTGGTCCACATCTTATAGTTTGTCCTGCTTCTGTTTTGGAAAACTGGGAAAGGGAACTAAAGAAGTGGTGCCCGTCCTTTTCTGTCCTTCAATATCACGGGTCTGCACGCGCAGCATACTGCAAGGAGTTGAATTCCTTGTCCAAGTCAGGATTGCCTCCTCCATTTAATGTTCTTCTCGTCTGTTATTCACTTTTCGAACGACACAGTGCACAGCAGAAAGATGATCGTAAAATCCTGAAGCGTTGGAAGTGGAGCTGTGTGCTGATGGATGAGGCACATGCTTTGAAGGACAAAAATAGTTTTAGGTGGAAAAATCTGATGTCTGTTGCTCGAAATGCGAACCAACGCTTGATGCTGACAGGGACGCCTCTTCAAAATGATCTACATGAGTTATGGTCTATGTTGGAGTTTATGATGCCTGATATTTTTGCTTCTGAAGATGTTGACTTAAAAAAGTTACTTAGTGCAGAAGATAGAGATTTAACTAGTCGTATGAAGTCTATATTAGGGCCATTTATTTTACGGCGCCTGAAATCTGATGTGATGCAGCAACTTGTTCGAAAAACACAGAAGGTTCAATATGTCGTAATGGAAAAGCAACAGGAACATGCTTACAAGGAAGCCATTGAGGAGTATCGTGCTGTTTCACAAGCTCGGTTAACAAAATGTTCTGATCTGAACTCAAAAAATGTTCTTGAAGTTTTACCTCGACGGCAAATAAATAATTATTTTGTTCAGTTCCGCAAGATTGCGAACCATCCCTTGTTGATAAGGCGAATTTATAATGATGAGGATGTTGTTCGCTTTGCTAGGAAACTACATCCAATAGGTGCTTTTGGCTTTGAATGTACTTTGGACAGAGTTATAGAGGAGCTTAAAAGTTACAATGACTTCTCCATTCACCGGCTTTTACTAAATTATGGTACCAATGATAGAAAGGGGATCCTATCAGATAAACATGTTATGCTTTCTGCGAAATGTCGGGCTTTAGCGGAACTTCTTCCATCACTAAAGAAGAGTGGTCACCGTGTTTTGATCTTTAGTCAATGGACATCAATGCTTGATATATTGGAGTGGGCTTTGGATGTCATTGGGTTGACCTACAAACGGCTTGATGGAAGCACACAGGTAGCAGAAAGGCAAACAATAGTCGACACATTCAATAATGATACTTCAATATTTGCATGCTTGCTTTCAACTAGAGCTGGTGGACAAGGCTTAAACTTAACTGGAGCTGATACGGTTGTGATTCATGACATGGATTTTAACCCACAGATTGATAGACAAGCAGAAGATCGTTGCCATCGAATTGGTCAAACAAAGCCTGTAACCGTATACAGGCTGGTGACAAAGGGAACAGTTGATGAAAATGTGTATGAGATTGCAAAGAGAAAGCTAGGATTAGATGCTGCTGTTCTAGAATCCATGGAAGAGATCAAAGAAGGGGACATGCCTGAGAAGACGATGGGAGAGATATTGTCTGCAATTTTGTTAAATTATTGA
Protein sequence

>MsaT001421.1

MKPELYEISDDEWENHSFKPSRVLKRPRSPPPPLESFYYKPPQPPPHPPSSSVTVSDDDDCFEIPRNTDNILDELDDAECDAAAAAAAPTARPGRRFIIDDEDEEDAGGSGGVDLYEIDSTEDEIEDAIEDANEDDLVGKALQKCARISVELKGELFGSSGAVCDRYSEVESSSVRIVTQDDVDVACGSEDSDFQPLLKPYQLVGVNFLLLLYRKRIGGAILADEMGLGKTVQAITYLTLLNHLHNDSGPHLIVCPASVLENWERELKKWCPSFSVLQYHGSARAAYCKELNSLSKSGLPPPFNVLLVCYSLFERHSAQQKDDRKILKRWKWSCVLMDEAHALKDKNSFRWKNLMSVARNANQRLMLTGTPLQNDLHELWSMLEFMMPDIFASEDVDLKKLLSAEDRDLTSRMKSILGPFILRRLKSDVMQQLVRKTQKVQYVVMEKQQEHAYKEAIEEYRAVSQARLTKCSDLNSKNVLEVLPRRQINNYFVQFRKIANHPLLIRRIYNDEDVVRFARKLHPIGAFGFECTLDRVIEELKSYNDFSIHRLLLNYGTNDRKGILSDKHVMLSAKCRALAELLPSLKKSGHRVLIFSQWTSMLDILEWALDVIGLTYKRLDGSTQVAERQTIVDTFNNDTSIFACLLSTRAGGQGLNLTGADTVVIHDMDFNPQIDRQAEDRCHRIGQTKPVTVYRLVTKGTVDENVYEIAKRKLGLDAAVLESMEEIKEGDMPEKTMGEILSAILLNY*