AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG001427

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG001427 MtrunA17_Chr1g0163051 97.126 661 15 3 1 659 1 659 0.0 1322
MsaG001427 MtrunA17_Chr1g0163041 81.559 526 88 3 143 659 53 578 0.0 864
MsaG001427 MtrunA17_Chr8g0368901 73.673 471 120 3 191 658 299 768 0.0 747
MsaG001427 MtrunA17_Chr8g0368901 36.667 120 62 4 100 214 148 258 7.26e-11 65.5
MsaG001427 MtrunA17_Chr8g0369301 58.581 606 194 8 101 658 152 748 0.0 712
MsaG001427 MtrunA17_Chr8g0368911 51.389 720 260 14 7 657 66 764 0.0 707
MsaG001427 MtrunA17_Chr6g0467531 56.944 576 200 12 103 646 5 564 0.0 625
MsaG001427 MtrunA17_Chr6g0467421 56.597 576 202 12 103 646 5 564 0.0 616
MsaG001427 MtrunA17_Chr6g0467381 56.944 576 200 13 103 646 5 564 0.0 616
MsaG001427 MtrunA17_Chr6g0467401 56.424 576 203 13 103 646 5 564 0.0 610
MsaG001427 MtrunA17_Chr3g0094881 53.554 605 234 11 88 659 49 639 0.0 605
MsaG001427 MtrunA17_Chr6g0467411 54.280 514 176 10 160 646 1 482 0.0 523
MsaG001427 MtrunA17_Chr2g0306961 52.500 520 161 11 153 646 4 463 5.77e-177 511
MsaG001427 MtrunA17_Chr7g0255181 41.818 495 265 13 158 646 203 680 7.22e-114 357
MsaG001427 MtrunA17_Chr3g0080761 36.929 547 310 14 123 645 98 633 6.24e-108 340
MsaG001427 MtrunA17_Chr5g0399801 37.050 556 308 15 105 651 543 1065 2.84e-106 347
MsaG001427 MtrunA17_Chr8g0354821 39.960 503 279 14 158 647 167 659 2.34e-105 335
MsaG001427 MtrunA17_Chr4g0003501 35.610 615 329 20 57 649 360 929 1.73e-101 331
MsaG001427 MtrunA17_Chr4g0003511 37.624 505 283 12 158 651 545 1028 6.68e-99 326
MsaG001427 MtrunA17_Chr6g0476111 38.923 483 267 13 189 651 196 670 1.27e-91 298
MsaG001427 MtrunA17_Chr6g0476121 38.509 483 269 13 189 651 145 619 6.18e-89 290
MsaG001427 MtrunA17_Chr7g0252211 38.763 485 266 14 189 651 258 733 8.65e-89 293
MsaG001427 MtrunA17_Chr1g0170651 32.341 504 285 12 157 644 57 520 8.64e-58 204
MsaG001427 MtrunA17_Chr7g0232811 29.814 483 242 14 192 651 107 515 1.17e-55 198
MsaG001427 MtrunA17_Chr7g0232981 30.022 463 246 11 192 652 107 493 5.85e-50 182
MsaG001427 MtrunA17_Chr7g0232841 28.247 485 231 17 192 650 110 503 1.98e-44 166
MsaG001427 MtrunA17_Chr1g0183321 25.161 310 162 10 396 650 430 724 2.01e-19 92.8
MsaG001427 MtrunA17_Chr1g0190311 27.157 313 157 12 396 652 327 624 3.44e-19 92.0
MsaG001427 MtrunA17_Chr5g0448331 37.179 156 85 3 217 366 267 415 3.94e-19 92.0
MsaG001427 MtrunA17_Chr6g0460921 27.477 222 134 6 440 659 215 411 2.89e-18 87.8
MsaG001427 MtrunA17_Chr4g0075321 28.804 184 117 3 463 644 869 1040 4.11e-18 89.0
MsaG001427 MtrunA17_Chr5g0403241 32.086 187 107 5 463 645 1312 1482 8.70e-18 88.2
MsaG001427 MtrunA17_Chr8g0373821 29.508 183 115 4 463 644 346 515 8.82e-18 87.0
MsaG001427 MtrunA17_Chr8g0389931 34.615 156 89 3 217 366 261 409 1.55e-17 87.0
MsaG001427 MtrunA17_Chr7g0232861 35.333 150 81 5 192 338 106 242 4.39e-17 81.3
MsaG001427 MtrunA17_Chr7g0262001 24.675 308 156 12 396 646 388 676 5.89e-16 81.6
MsaG001427 MtrunA17_Chr7g0232761 34.591 159 84 6 192 345 123 266 5.40e-14 72.8
MsaG001427 MtrunA17_Chr3g0143561 32.000 150 91 3 217 366 187 325 8.46e-14 73.9
MsaG001427 MtrunA17_Chr7g0232751 43.077 65 37 0 587 651 31 95 1.81e-12 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG001427 AT4G13460.1 54.834 662 270 10 10 659 6 650 0.0 706
MsaG001427 AT4G13460.2 54.834 662 270 10 10 659 6 650 0.0 706
MsaG001427 AT2G33290.1 52.680 653 265 11 21 659 29 651 0.0 676
MsaG001427 AT2G33290.3 52.680 653 265 11 21 659 29 651 0.0 676
MsaG001427 AT2G33290.4 52.680 653 265 11 21 659 29 651 0.0 676
MsaG001427 AT2G33290.2 52.680 653 265 11 21 659 29 651 0.0 676
MsaG001427 AT5G04940.2 38.889 504 277 12 158 647 155 641 6.51e-104 330
MsaG001427 AT5G04940.1 38.889 504 277 12 158 647 155 641 6.51e-104 330
MsaG001427 AT1G73100.1 40.430 465 254 12 193 647 189 640 9.18e-101 322
MsaG001427 AT2G22740.2 36.328 512 293 9 158 651 270 766 2.92e-96 313
MsaG001427 AT2G22740.1 36.328 512 293 9 158 651 270 766 2.92e-96 313
MsaG001427 AT2G35160.5 35.088 570 297 15 110 651 210 734 3.89e-96 312
MsaG001427 AT2G35160.2 35.088 570 297 15 110 651 246 770 1.14e-95 312
MsaG001427 AT2G35160.3 35.088 570 297 15 110 651 246 770 1.14e-95 312
MsaG001427 AT2G35160.1 35.088 570 297 15 110 651 246 770 1.14e-95 312
MsaG001427 AT2G35160.4 35.088 570 297 15 110 651 246 770 1.14e-95 312
MsaG001427 AT5G13960.1 36.253 491 273 16 189 653 126 602 8.98e-88 287
MsaG001427 AT5G13960.2 36.383 470 262 14 209 653 11 468 1.26e-86 280
MsaG001427 AT1G17770.1 37.124 466 269 12 194 647 209 662 2.32e-80 269
MsaG001427 AT2G24740.1 36.255 502 284 14 158 654 262 732 1.93e-76 260
MsaG001427 AT5G47150.1 36.101 277 162 6 92 366 61 324 1.17e-37 143
MsaG001427 AT5G47160.1 37.674 215 122 6 151 362 202 407 9.80e-29 119
MsaG001427 AT5G47160.3 37.674 215 122 6 151 362 202 407 9.80e-29 119
MsaG001427 AT5G47160.2 37.674 215 122 6 151 362 202 407 9.80e-29 119
MsaG001427 AT2G05900.1 36.257 171 104 3 254 424 26 191 2.04e-21 95.9
MsaG001427 AT2G23740.3 32.632 190 104 6 463 645 1184 1356 3.79e-21 99.4
MsaG001427 AT2G23740.6 32.632 190 104 6 463 645 1184 1356 3.79e-21 99.4
MsaG001427 AT2G23740.2 32.632 190 104 6 463 645 1184 1356 3.79e-21 99.4
MsaG001427 AT2G23740.1 32.632 190 104 6 463 645 1177 1349 3.81e-21 99.4
MsaG001427 AT3G03750.2 30.288 208 119 6 439 645 141 323 2.98e-19 90.5
MsaG001427 AT1G57820.1 35.669 157 87 4 217 366 277 426 5.88e-18 88.6
MsaG001427 AT1G57820.3 35.669 157 87 4 217 366 277 426 5.88e-18 88.6
MsaG001427 AT3G04380.3 24.183 306 162 11 396 646 130 420 9.54e-18 87.0
MsaG001427 AT3G04380.1 24.183 306 162 11 396 646 146 436 1.01e-17 87.0
MsaG001427 AT3G04380.4 24.183 306 162 11 396 646 146 436 1.18e-17 87.0
MsaG001427 AT3G04380.2 24.183 306 162 11 396 646 119 409 1.22e-17 86.7
MsaG001427 AT3G04380.6 24.183 306 162 11 396 646 119 409 1.33e-17 86.7
MsaG001427 AT3G04380.5 24.183 306 162 11 396 646 103 393 1.41e-17 86.3
MsaG001427 AT1G57820.2 35.065 154 89 4 217 366 277 423 7.59e-17 85.1
MsaG001427 AT5G39550.1 33.121 157 91 4 217 366 262 411 6.49e-15 78.6
MsaG001427 AT2G23740.4 33.108 148 75 6 463 603 1184 1314 1.32e-14 78.2
MsaG001427 AT2G23740.5 33.108 148 75 6 463 603 1184 1314 1.32e-14 78.2
MsaG001427 AT1G04050.2 25.076 331 160 11 384 646 387 697 1.66e-14 77.8
MsaG001427 AT1G04050.1 25.076 331 160 11 384 646 387 697 1.66e-14 77.8
MsaG001427 AT1G04050.3 25.076 331 160 11 384 646 283 593 1.90e-14 77.4
MsaG001427 AT1G66040.1 32.867 143 83 2 217 353 262 397 8.79e-14 75.1
MsaG001427 AT1G66050.1 32.867 143 83 2 217 353 262 397 9.60e-14 75.1
MsaG001427 AT5G43990.15 27.011 174 109 4 475 646 526 683 3.44e-13 73.6
MsaG001427 AT5G43990.10 27.011 174 109 4 475 646 526 683 3.44e-13 73.6
MsaG001427 AT5G43990.16 27.586 174 108 4 475 646 503 660 3.54e-13 73.2
MsaG001427 AT5G43990.5 27.586 174 108 4 475 646 503 660 3.54e-13 73.2
MsaG001427 AT5G43990.17 27.586 174 108 4 475 646 503 660 3.54e-13 73.2
MsaG001427 AT5G43990.14 27.586 174 108 4 475 646 503 660 3.54e-13 73.2
MsaG001427 AT5G43990.3 27.011 174 109 4 475 646 523 680 4.07e-13 73.2
MsaG001427 AT5G43990.11 27.011 174 109 4 475 646 523 680 4.07e-13 73.2
MsaG001427 AT5G43990.1 27.011 174 109 4 475 646 523 680 4.07e-13 73.2
MsaG001427 AT5G43990.4 27.011 174 109 4 475 646 523 680 4.07e-13 73.2
MsaG001427 AT5G43990.9 27.011 174 109 4 475 646 546 703 4.42e-13 73.2
MsaG001427 AT5G43990.2 27.011 174 109 4 475 646 546 703 4.42e-13 73.2
MsaG001427 AT5G43990.7 27.011 174 109 4 475 646 434 591 5.11e-13 72.8
MsaG001427 AT5G43990.8 27.586 174 108 4 475 646 454 611 6.19e-13 72.4
MsaG001427 AT5G43990.12 27.586 174 108 4 475 646 454 611 6.19e-13 72.4
MsaG001427 AT5G43990.6 27.586 174 108 4 475 646 454 611 6.19e-13 72.4
MsaG001427 AT5G43990.13 27.586 174 108 4 475 646 454 611 6.19e-13 72.4
MsaG001427 AT1G57800.2 31.646 158 93 5 217 366 289 439 2.91e-12 70.5
MsaG001427 AT1G57800.1 32.857 140 80 4 217 349 289 421 6.89e-12 69.3

Find 178 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCTACTAGTGTGATTGTTTC+TGG 0.079078 1:+19992477 MsaT001427.1:CDS
CTAGGTTCAGACTTAGATTT+AGG 0.191105 1:-19991855 None:intergenic
TTAGATTTAGGTTTAGATTT+TGG 0.203811 1:-19991843 None:intergenic
CGGAACCGTGTTACACAAAA+AGG 0.211826 1:+19993179 MsaT001427.1:CDS
TTATGATTCGGTTCGTGTTT+TGG 0.278166 1:+19992206 MsaT001427.1:CDS
CTTGCATCTGCATTTGCATC+TGG 0.286549 1:-19992021 None:intergenic
GGGAGACTGGTTGGGGAGTT+AGG 0.302694 1:+19993234 MsaT001427.1:CDS
GTATCCATCAATTCCTCCTT+TGG 0.310358 1:+19993439 MsaT001427.1:CDS
AGAGAGTTCAGCCTTGATTA+TGG 0.322787 1:+19993617 MsaT001427.1:CDS
GCAAGCCACATTCCTCATTC+TGG 0.324681 1:-19993480 None:intergenic
GGTTGGAAGTGTTTAGATCT+AGG 0.329004 1:+19993213 MsaT001427.1:CDS
AGGATTGTTGGCGCGATTCC+TGG 0.334668 1:+19992333 MsaT001427.1:CDS
CACCAGCAGGAGGGGCATTA+TGG 0.334995 1:-19992080 None:intergenic
GATGTTGGTAAAAGTGGTTT+TGG 0.352738 1:+19992744 MsaT001427.1:CDS
AGCTGGGATTGATTATTTGC+CGG 0.352953 1:+19992425 MsaT001427.1:CDS
GAAGGTACTTCTTCGACTTC+GGG 0.363101 1:+19992672 MsaT001427.1:CDS
GTCAGTTTCGTGACGTTGTT+AGG 0.364736 1:+19992169 MsaT001427.1:CDS
ATGGAGAGGAGTATGCATTA+TGG 0.365705 1:+19992615 MsaT001427.1:CDS
TTGTGTGTGGTTGGTTTACA+TGG 0.365764 1:+19992393 MsaT001427.1:CDS
TTTAAGTTATGGAGGATTGA+TGG 0.371705 1:+19992777 MsaT001427.1:CDS
GGCGGCGTCGACGCCGGCGC+CGG 0.374770 1:-19991917 None:intergenic
TAGATCTAGGGAGACTGGTT+GGG 0.375144 1:+19993226 MsaT001427.1:CDS
TGCATTATGGGATTGAGGTT+AGG 0.376465 1:+19992628 MsaT001427.1:CDS
GCTGATGGAAATGTTTGGTT+TGG 0.379538 1:-19991721 None:intergenic
GTTTGGATTATGAGGATTCA+TGG 0.380762 1:-19991704 None:intergenic
GCATTATGGGATTGAGGTTA+GGG 0.381831 1:+19992629 MsaT001427.1:CDS
GTATGGAGCCTGTCAGTATA+AGG 0.386131 1:+19992904 MsaT001427.1:CDS
TCGGAAAATCTCCGCCATTC+TGG 0.390186 1:-19991938 None:intergenic
TTCAAACAGGTGGACGGAAT+GGG 0.394764 1:+19993376 MsaT001427.1:CDS
TGAAGGTACTTCTTCGACTT+CGG 0.398545 1:+19992671 MsaT001427.1:CDS
ATGTTTATGATGGTTTGTAT+AGG 0.399652 1:+19992703 MsaT001427.1:CDS
CATTCTGGCGGCGTCGACGC+CGG 0.406416 1:-19991923 None:intergenic
GACGAGGATGGTTTATGATT+CGG 0.406490 1:+19992194 MsaT001427.1:CDS
AATGACGGATGTTGGGTTAC+CGG 0.408229 1:+19992140 MsaT001427.1:CDS
CGGCGTCGACGCCGCCAGAA+TGG 0.408674 1:+19991924 MsaT001427.1:CDS
TCATCCCGAGGCTCTTGCTT+CGG 0.409571 1:-19991775 None:intergenic
TAAATGCACCAGCTTGAATA+AGG 0.411317 1:-19993265 None:intergenic
TGGAGAGGAGTATGCATTAT+GGG 0.415278 1:+19992616 MsaT001427.1:CDS
GTCGACGCCGGCGCCGGTGG+TGG 0.415602 1:-19991911 None:intergenic
TGTATAGGATTATTGAATGC+TGG 0.416427 1:+19992718 MsaT001427.1:CDS
GTAGTTGTCCGCCTCATTGT+CGG 0.419366 1:+19993159 MsaT001427.1:CDS
ACAAACTGAACCATCACATT+AGG 0.419575 1:-19993524 None:intergenic
GCGATTCCTGGGATTTGTAT+TGG 0.420322 1:+19992345 MsaT001427.1:CDS
CATTTATATGCGAGTACACT+GGG 0.423254 1:+19993282 MsaT001427.1:CDS
AAATCCAAAGGAGGAATTGA+TGG 0.424705 1:-19993443 None:intergenic
AGAAGCCCGCTTTGACTGTA+AGG 0.435492 1:-19993095 None:intergenic
GACGCCGGCGCCGGTGGTGG+TGG 0.437295 1:-19991908 None:intergenic
TCTTGATATTTCCAAGGGTA+TGG 0.441501 1:+19992887 MsaT001427.1:CDS
GGTAAGGTTTATGTTTATGA+TGG 0.452304 1:+19992693 MsaT001427.1:CDS
GATTGATGGTCAAGCTAAGA+TGG 0.453129 1:+19992791 MsaT001427.1:CDS
TCACGAAACTGACGCTGATC+CGG 0.454247 1:-19992159 None:intergenic
GGGGTGCTGATGGAAATGTT+TGG 0.454594 1:-19991726 None:intergenic
AATCGCGATAAGAGGATTGT+TGG 0.454649 1:+19992321 MsaT001427.1:CDS
GTTGGAAGTGTTTAGATCTA+GGG 0.456470 1:+19993214 MsaT001427.1:CDS
GTGTTTAGATCTAGGGAGAC+TGG 0.456502 1:+19993221 MsaT001427.1:CDS
TGTGGTTGAATCGCGATAAG+AGG 0.456612 1:+19992313 MsaT001427.1:CDS
CTTGTTCGAATGACGGATGT+TGG 0.459771 1:+19992132 MsaT001427.1:CDS
CTCTTGATGTATCCAGAATG+AGG 0.465074 1:+19993468 MsaT001427.1:CDS
GATTGTTTCTGGTGGGTATG+AGG 0.465601 1:+19992488 MsaT001427.1:CDS
TTGTTCGAATGACGGATGTT+GGG 0.470012 1:+19992133 MsaT001427.1:CDS
AATGTTTGGTTTGGATTATG+AGG 0.470379 1:-19991712 None:intergenic
GGCGTCGACGCCGGCGCCGG+TGG 0.471937 1:-19991914 None:intergenic
AGATCTAGGGAGACTGGTTG+GGG 0.471997 1:+19993227 MsaT001427.1:CDS
GTTATTATTTACTCGGGTCA+TGG 0.472036 1:+19992531 MsaT001427.1:CDS
AGGTGATGTTATTATTTACT+CGG 0.473486 1:+19992524 MsaT001427.1:CDS
TACTTCTTCGACTTCGGGTA+AGG 0.474074 1:+19992677 MsaT001427.1:CDS
TGGTTTGATGTTGGTAAAAG+TGG 0.475144 1:+19992738 MsaT001427.1:CDS
GCCCTTGAGAAACAAAATCT+AGG 0.476619 1:-19991873 None:intergenic
GAACAACCAACCACCACCAC+CGG 0.476859 1:+19991898 MsaT001427.1:CDS
GGATTGTTGGCGCGATTCCT+GGG 0.482675 1:+19992334 MsaT001427.1:CDS
TTGGCTATGGCTGAAGAAGA+AGG 0.484128 1:+19992225 MsaT001427.1:CDS
AGGATGGAGTTGTGTGTGGT+TGG 0.488005 1:+19992384 MsaT001427.1:CDS
TACCATAATGCCCCTCCTGC+TGG 0.488379 1:+19992078 MsaT001427.1:CDS
CCGGCGCCGGTGGTGGTGGT+TGG 0.488719 1:-19991904 None:intergenic
GAGTATGCATTATGGGATTG+AGG 0.491128 1:+19992623 MsaT001427.1:CDS
CATGAAAGTGGGAATGCGAC+AGG 0.494424 1:+19993008 MsaT001427.1:CDS
GCTGGGATTGATTATTTGCC+GGG 0.495001 1:+19992426 MsaT001427.1:CDS
GCTATTGTTCCGGTTCCTGC+TGG 0.497566 1:+19992045 MsaT001427.1:CDS
GCATTTATATGCGAGTACAC+TGG 0.498857 1:+19993281 MsaT001427.1:CDS
TTTCAAACAGGTGGACGGAA+TGG 0.498875 1:+19993375 MsaT001427.1:CDS
TACACCGAAGCAAGAGCCTC+GGG 0.501130 1:+19991771 MsaT001427.1:CDS
AAAAGGGCTTAAACATAGGT+TGG 0.501382 1:+19993196 MsaT001427.1:CDS
TTATTCTCACCAGCAGGAAC+CGG 0.503916 1:-19992054 None:intergenic
AATTTATCTCTGATTCAAGA+TGG 0.504650 1:+19993401 MsaT001427.1:CDS
TATATCTCTTGATATTTCCA+AGG 0.512997 1:+19992881 MsaT001427.1:CDS
CGCAACCTCACCAGCAGGAG+GGG 0.513548 1:-19992088 None:intergenic
ATTGAGGTTAGGGTTATTCG+TGG 0.514365 1:+19992639 MsaT001427.1:CDS
ATTCGTGGTGTGAGGTATGA+AGG 0.515290 1:+19992654 MsaT001427.1:CDS
TCACATTATGCTGTTTGCGA+TGG 0.516973 1:+19993577 MsaT001427.1:CDS
GGATATTGAAATTCTTGCGT+TGG 0.517003 1:-19991799 None:intergenic
GGTGATGTTATTATTTACTC+GGG 0.517521 1:+19992525 MsaT001427.1:CDS
TTACTCGGGTCATGGTGGAC+AGG 0.519127 1:+19992539 MsaT001427.1:CDS
TACATGGTCAACCTCAAGCT+GGG 0.522010 1:+19992409 MsaT001427.1:CDS
CTAGTGTGATTGTTTCTGGT+GGG 0.526236 1:+19992481 MsaT001427.1:CDS
AGCATAGGAAAAGGAATACC+AGG 0.526910 1:-19991748 None:intergenic
ACTAGTGTGATTGTTTCTGG+TGG 0.533046 1:+19992480 MsaT001427.1:CDS
TGGTAATTATTCTCACCAGC+AGG 0.536793 1:-19992060 None:intergenic
CAAAGCGGGCTTCTCTTGAA+AGG 0.538573 1:+19993104 MsaT001427.1:CDS
ATTGAATGCTGGTTTGATGT+TGG 0.540002 1:+19992729 MsaT001427.1:CDS
TCAAGGCTGAACTCTCTCAA+TGG 0.540411 1:-19993611 None:intergenic
ACAATCACACTAGTAGCAAT+CGG 0.540891 1:-19992471 None:intergenic
TTAGATCTAGGGAGACTGGT+TGG 0.541898 1:+19993225 MsaT001427.1:CDS
CCAACCACCACCACCGGCGC+CGG 0.541973 1:+19991904 MsaT001427.1:CDS
ATATCTCTTGATATTTCCAA+GGG 0.542298 1:+19992882 MsaT001427.1:CDS
TCATTGGACCTTATTCAAGC+TGG 0.545200 1:+19993257 MsaT001427.1:CDS
ACTTTCCTTACAGTCAAAGC+GGG 0.545405 1:+19993090 MsaT001427.1:CDS
TTCATCAGAAGCTGGAAGGT+GGG 0.546457 1:+19992583 MsaT001427.1:CDS
TTCGGTGTAAGCATAGGAAA+AGG 0.548026 1:-19991757 None:intergenic
TTCCGACGCTCATTCACTGA+AGG 0.548549 1:+19991955 MsaT001427.1:CDS
TAATGCCCCTCCTGCTGGTG+AGG 0.550096 1:+19992083 MsaT001427.1:CDS
CTTTCATGAAACACAAAATG+TGG 0.552365 1:-19992993 None:intergenic
TTACATGGTCAACCTCAAGC+TGG 0.553493 1:+19992408 MsaT001427.1:CDS
TGGTTGGGGAGTTAGGTCAT+TGG 0.553990 1:+19993241 MsaT001427.1:CDS
TATTAATGAATCACCACTCA+TGG 0.554494 1:-19993339 None:intergenic
ACTGACAGGCTCCATACCCT+TGG 0.556578 1:-19992898 None:intergenic
GCTTCTCTTGAAAGGAAAGC+CGG 0.556611 1:+19993112 MsaT001427.1:CDS
ATTGATGGTCAAGCTAAGAT+GGG 0.559091 1:+19992792 MsaT001427.1:CDS
TTTCATCAGAAGCTGGAAGG+TGG 0.563122 1:+19992582 MsaT001427.1:CDS
CGTTGTTAGGAGGACGAGGA+TGG 0.566575 1:+19992182 MsaT001427.1:CDS
ACATTCGCAACCTCACCAGC+AGG 0.568299 1:-19992093 None:intergenic
TCAAACAGGTGGACGGAATG+GGG 0.569248 1:+19993377 MsaT001427.1:CDS
GTGGACAGGATAAGAATTCG+AGG 0.571781 1:+19992553 MsaT001427.1:CDS
CCGGGGAGTATGAGTTCGAA+TGG 0.571907 1:+19992444 MsaT001427.1:CDS
ACAGGATAAGAATTCGAGGC+AGG 0.572812 1:+19992557 MsaT001427.1:CDS
CATCAGCTACACCATAATCA+AGG 0.573838 1:-19993628 None:intergenic
CGATGAGGAGTCGAGGATTG+TGG 0.574012 1:+19992295 MsaT001427.1:CDS
TGGCTATGGCTGAAGAAGAA+GGG 0.574030 1:+19992226 MsaT001427.1:CDS
GTATAGGATGGAGTTGTGTG+TGG 0.574388 1:+19992380 MsaT001427.1:CDS
GGCTGAACTCTCTCAATGGA+GGG 0.575518 1:-19993607 None:intergenic
GGAAGGTGGGAATCTTGCTA+TGG 0.576684 1:+19992596 MsaT001427.1:CDS
TAAAGAGCTTGTTCGAATGA+CGG 0.576987 1:+19992125 MsaT001427.1:CDS
AAATGTGGAAAAGTAGCCCT+AGG 0.577130 1:-19992978 None:intergenic
TCTTGCTTCGGTGTAAGCAT+AGG 0.581620 1:-19991763 None:intergenic
TGCGACAGGTTGTCAATGTG+AGG 0.581743 1:+19993022 MsaT001427.1:CDS
TAGCTCAACTCCTAATGTGA+TGG 0.586959 1:+19993514 MsaT001427.1:CDS
GAAAATCTCCGCCATTCTGG+CGG 0.591102 1:-19991935 None:intergenic
CTGGTGAGGTTGCGAATGTA+CGG 0.592271 1:+19992097 MsaT001427.1:CDS
GTGACGTTGTTAGGAGGACG+AGG 0.592386 1:+19992178 MsaT001427.1:CDS
TTACACCGAAGCAAGAGCCT+CGG 0.592731 1:+19991770 MsaT001427.1:CDS
CGTCGACGCCGCCAGAATGG+CGG 0.594115 1:+19991927 MsaT001427.1:CDS
AGGCTGAACTCTCTCAATGG+AGG 0.596120 1:-19993608 None:intergenic
AGTTTCGTGACGTTGTTAGG+AGG 0.598043 1:+19992172 MsaT001427.1:CDS
TCGCAACCTCACCAGCAGGA+GGG 0.598600 1:-19992089 None:intergenic
AGATGCAAGAGCTATTGTTC+CGG 0.602129 1:+19992035 MsaT001427.1:CDS
TCAAGAGATATAACACACAT+AGG 0.602330 1:-19992870 None:intergenic
TCTTGCGTTGGTTCATCCCG+AGG 0.604080 1:-19991787 None:intergenic
GGAGGAATTGATGGATACGA+AGG 0.604652 1:-19993434 None:intergenic
TTAGGGTTATTCGTGGTGTG+AGG 0.607128 1:+19992646 MsaT001427.1:CDS
ATCCTTCAGTGAATGAGCGT+CGG 0.615611 1:-19991957 None:intergenic
ACATCACCAATACAAATCCC+AGG 0.617403 1:-19992351 None:intergenic
TCAGAAATTATGACCATGAG+TGG 0.618462 1:+19993326 MsaT001427.1:CDS
ACACTGGGGTTGTTCTGACA+AGG 0.627478 1:+19993297 MsaT001427.1:CDS
TTCGCAACCTCACCAGCAGG+AGG 0.628056 1:-19992090 None:intergenic
CCATTCGAACTCATACTCCC+CGG 0.628119 1:-19992444 None:intergenic
GTGGGAATCTTGCTATGGAG+AGG 0.630151 1:+19992601 MsaT001427.1:CDS
TATGAGGATGATGTTGATGA+AGG 0.637266 1:+19992504 MsaT001427.1:CDS
GGAATTGATGGATACGAAGG+AGG 0.638458 1:-19993431 None:intergenic
GCGACAGGTTGTCAATGTGA+GGG 0.638640 1:+19993023 MsaT001427.1:CDS
GACTTTCCTTACAGTCAAAG+CGG 0.639876 1:+19993089 MsaT001427.1:CDS
ATCGCAAACAGCATAATGTG+AGG 0.644756 1:-19993575 None:intergenic
AAATAATCAATCCCAGCTTG+AGG 0.645920 1:-19992420 None:intergenic
GTAGCTGATGATGAGTTGAC+TGG 0.647668 1:+19993641 MsaT001427.1:CDS
CACATAGGTTTATAACACAA+AGG 0.649300 1:-19992855 None:intergenic
CTGGGATTGATTATTTGCCG+GGG 0.652302 1:+19992427 MsaT001427.1:CDS
TCGCAAACAGCATAATGTGA+GGG 0.654166 1:-19993574 None:intergenic
TGAAAGCTTCTACTACGATG+AGG 0.657632 1:+19992280 MsaT001427.1:CDS
TGAGGCGGACAACTACAAGA+AGG 0.667023 1:-19993152 None:intergenic
CACTGGGGTTGTTCTGACAA+GGG 0.671410 1:+19993298 MsaT001427.1:CDS
ATTATTTACTCGGGTCATGG+TGG 0.676989 1:+19992534 MsaT001427.1:CDS
TCTACTACGATGAGGAGTCG+AGG 0.680653 1:+19992288 MsaT001427.1:CDS
ACACGGTTCCGACAATGAGG+CGG 0.681770 1:-19993167 None:intergenic
GGAAGATACTCAAAACACAT+CGG 0.687151 1:-19992957 None:intergenic
GCATAGGAAAAGGAATACCA+GGG 0.688262 1:-19991747 None:intergenic
GTAACACGGTTCCGACAATG+AGG 0.703986 1:-19993170 None:intergenic
CATAGGAAAAGGAATACCAG+GGG 0.729249 1:-19991746 None:intergenic
TGTATCCAGAATGAGGAATG+TGG 0.734351 1:+19993475 MsaT001427.1:CDS
ATTTATATGCGAGTACACTG+GGG 0.744900 1:+19993283 MsaT001427.1:CDS
ATAGGAAAAGGAATACCAGG+GGG 0.791262 1:-19991745 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr1 gene 19991706 19993685 19991706 ID=MsaG001427
Chr1 mRNA 19991706 19993685 19991706 ID=MsaT001427.1;Parent=MsaG001427
Chr1 exon 19991706 19993685 19991706 ID=MsaT001427.1.exon1;Parent=MsaT001427.1
Chr1 CDS 19991706 19993685 19991706 ID=cds.MsaT001427.1;Parent=MsaT001427.1
Gene Sequence

>MsaT001427.1

ATGAATCCTCATAATCCAAACCAAACATTTCCATCAGCACCCCCTGGTATTCCTTTTCCTATGCTTACACCGAAGCAAGAGCCTCGGGATGAACCAACGCAAGAATTTCAATATCCAAACCTAGAAAATTTACCGAACCAAAATCTAAACCTAAATCTAAGTCTGAACCTAGATTTTGTTTCTCAAGGGCTAGAACAACCAACCACCACCACCGGCGCCGGCGTCGACGCCGCCAGAATGGCGGAGATTTTCCGACGCTCATTCACTGAAGGATTACAACGACAGATTCAAAATAATGATGCTGTTGATGAAAATCCAGATGCAAATGCAGATGCAAGAGCTATTGTTCCGGTTCCTGCTGGTGAGAATAATTACCATAATGCCCCTCCTGCTGGTGAGGTTGCGAATGTACGGAAACATAAAGAGCTTGTTCGAATGACGGATGTTGGGTTACCGGATCAGCGTCAGTTTCGTGACGTTGTTAGGAGGACGAGGATGGTTTATGATTCGGTTCGTGTTTTGGCTATGGCTGAAGAAGAAGGGAATTTTAATGTTAGGAGAGTGAGAAGTGATTTGAAAGCTTCTACTACGATGAGGAGTCGAGGATTGTGGTTGAATCGCGATAAGAGGATTGTTGGCGCGATTCCTGGGATTTGTATTGGTGATGTGTTTTTGTATAGGATGGAGTTGTGTGTGGTTGGTTTACATGGTCAACCTCAAGCTGGGATTGATTATTTGCCGGGGAGTATGAGTTCGAATGGTGAACCGATTGCTACTAGTGTGATTGTTTCTGGTGGGTATGAGGATGATGTTGATGAAGGTGATGTTATTATTTACTCGGGTCATGGTGGACAGGATAAGAATTCGAGGCAGGTTTTTCATCAGAAGCTGGAAGGTGGGAATCTTGCTATGGAGAGGAGTATGCATTATGGGATTGAGGTTAGGGTTATTCGTGGTGTGAGGTATGAAGGTACTTCTTCGACTTCGGGTAAGGTTTATGTTTATGATGGTTTGTATAGGATTATTGAATGCTGGTTTGATGTTGGTAAAAGTGGTTTTGGTGTTTTTAAGTTTAAGTTATGGAGGATTGATGGTCAAGCTAAGATGGGTAGTTTGATTTTAAAGGAGGCTTTTTTACTTAGGAGAGATCCTTTGTGTTATAAACCTATGTGTGTTATATCTCTTGATATTTCCAAGGGTATGGAGCCTGTCAGTATAAGGCTTTTTAATGATATTGATAAATGTAATGATCCGATGTGTTTTGAGTATCTTCCTAGGGCTACTTTTCCACATTTTGTGTTTCATGAAAGTGGGAATGCGACAGGTTGTCAATGTGAGGGCTTTTGTGAGGAAGGATGTTTTTGTTTCATAAAGAATGGAAATGACTTTCCTTACAGTCAAAGCGGGCTTCTCTTGAAAGGAAAGCCGGTCATTTTTGAATGTGGTCCTTCTTGTAGTTGTCCGCCTCATTGTCGGAACCGTGTTACACAAAAAGGGCTTAAACATAGGTTGGAAGTGTTTAGATCTAGGGAGACTGGTTGGGGAGTTAGGTCATTGGACCTTATTCAAGCTGGTGCATTTATATGCGAGTACACTGGGGTTGTTCTGACAAGGGAGCAATCAGAAATTATGACCATGAGTGGTGATTCATTAATATATCCGAATCGTTTTTCAAACAGGTGGACGGAATGGGGTAATTTATCTCTGATTCAAGATGGTTATGTGCCTCCTTCGTATCCATCAATTCCTCCTTTGGATTTTTCTCTTGATGTATCCAGAATGAGGAATGTGGCTTGCTATATAAGTCATAGCTCAACTCCTAATGTGATGGTTCAGTTTGTTTTGTATGATCATAATAATTTGATGTTCCCTCACATTATGCTGTTTGCGATGGAGAACATCCCTCCATTGAGAGAGTTCAGCCTTGATTATGGTGTAGCTGATGATGAGTTGACTGGCAAGCTCGTAATATGCAACTGA
Protein sequence

>MsaT001427.1

MNPHNPNQTFPSAPPGIPFPMLTPKQEPRDEPTQEFQYPNLENLPNQNLNLNLSLNLDFVSQGLEQPTTTTGAGVDAARMAEIFRRSFTEGLQRQIQNNDAVDENPDANADARAIVPVPAGENNYHNAPPAGEVANVRKHKELVRMTDVGLPDQRQFRDVVRRTRMVYDSVRVLAMAEEEGNFNVRRVRSDLKASTTMRSRGLWLNRDKRIVGAIPGICIGDVFLYRMELCVVGLHGQPQAGIDYLPGSMSSNGEPIATSVIVSGGYEDDVDEGDVIIYSGHGGQDKNSRQVFHQKLEGGNLAMERSMHYGIEVRVIRGVRYEGTSSTSGKVYVYDGLYRIIECWFDVGKSGFGVFKFKLWRIDGQAKMGSLILKEAFLLRRDPLCYKPMCVISLDISKGMEPVSIRLFNDIDKCNDPMCFEYLPRATFPHFVFHESGNATGCQCEGFCEEGCFCFIKNGNDFPYSQSGLLLKGKPVIFECGPSCSCPPHCRNRVTQKGLKHRLEVFRSRETGWGVRSLDLIQAGAFICEYTGVVLTREQSEIMTMSGDSLIYPNRFSNRWTEWGNLSLIQDGYVPPSYPSIPPLDFSLDVSRMRNVACYISHSSTPNVMVQFVLYDHNNLMFPHIMLFAMENIPPLREFSLDYGVADDELTGKLVICN*