AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG001430

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG001430 MtrunA17_Chr8g0383631 75.427 293 23 4 205 448 142 434 2.86e-140 433
MsaG001430 MtrunA17_Chr3g0079701 73.720 293 28 4 205 448 131 423 4.56e-137 424
MsaG001430 MtrunA17_Chr6g0476171 78.947 247 49 2 530 776 2 245 7.79e-137 404
MsaG001430 MtrunA17_Chr1g0163101 68.421 190 34 2 1 184 1 170 1.13e-80 256
MsaG001430 MtrunA17_Chr1g0163101 68.367 98 27 1 456 553 32 125 2.03e-38 141
MsaG001430 MtrunA17_Chr6g0476181 72.549 153 42 0 32 184 1 153 1.18e-70 228
MsaG001430 MtrunA17_Chr6g0476181 87.671 73 9 0 456 528 1 73 6.85e-39 141
MsaG001430 MtrunA17_Chr1g0163141 74.534 161 34 2 31 184 9 169 1.95e-70 229
MsaG001430 MtrunA17_Chr1g0163141 85.333 75 11 0 456 530 10 84 1.02e-37 139
MsaG001430 MtrunA17_Chr1g0163131 52.838 229 86 3 5 228 6 217 1.15e-68 229
MsaG001430 MtrunA17_Chr1g0163131 36.223 323 155 7 456 776 33 306 1.27e-47 171
MsaG001430 MtrunA17_Chr1g0162161 66.456 158 31 2 32 184 3 143 1.78e-62 206
MsaG001430 MtrunA17_Chr1g0162161 67.347 98 28 1 456 553 3 96 1.25e-37 138
MsaG001430 MtrunA17_Chr1g0163201 34.286 385 177 11 457 776 19 392 6.69e-55 194
MsaG001430 MtrunA17_Chr1g0163201 34.694 147 73 4 33 157 19 164 5.85e-19 90.1
MsaG001430 MtrunA17_Chr1g0163081 61.392 158 34 3 32 184 5 140 2.64e-52 178
MsaG001430 MtrunA17_Chr1g0163081 68.367 98 27 1 456 553 5 98 6.35e-39 141
MsaG001430 MtrunA17_Chr1g0163121 57.862 159 34 3 31 184 9 139 1.08e-49 171
MsaG001430 MtrunA17_Chr1g0163121 78.205 78 17 0 452 529 6 83 5.07e-37 135
MsaG001430 MtrunA17_Chr1g0163161 60.274 146 34 3 45 184 1 128 6.54e-47 163
MsaG001430 MtrunA17_Chr1g0163161 61.176 85 29 1 469 553 1 81 3.69e-28 110
MsaG001430 MtrunA17_Chr1g0163261 37.818 275 111 7 457 676 25 294 1.08e-43 160
MsaG001430 MtrunA17_Chr1g0163261 32.420 219 136 4 17 226 9 224 1.03e-25 108
MsaG001430 MtrunA17_Chr1g0171611 39.024 246 112 6 457 666 25 268 1.74e-42 156
MsaG001430 MtrunA17_Chr1g0171611 34.940 166 102 3 17 180 9 170 1.01e-24 105
MsaG001430 MtrunA17_Chr1g0163231 37.984 258 110 6 457 666 25 280 7.26e-41 152
MsaG001430 MtrunA17_Chr1g0163231 39.766 171 78 5 17 185 9 156 6.13e-27 111
MsaG001430 MtrunA17_Chr1g0163151 38.839 224 128 6 554 776 6 221 8.65e-41 149
MsaG001430 MtrunA17_Chr1g0163111 37.946 224 123 7 562 776 12 228 2.38e-39 145
MsaG001430 MtrunA17_Chr1g0162151 38.912 239 108 8 540 775 3 206 7.93e-38 140
MsaG001430 MtrunA17_Chr1g0163251 34.643 280 114 7 457 676 21 291 3.38e-37 144
MsaG001430 MtrunA17_Chr1g0163251 38.710 217 108 6 26 226 14 221 1.31e-29 122
MsaG001430 MtrunA17_Chr1g0163181 35.573 253 125 9 554 776 185 429 1.12e-34 137
MsaG001430 MtrunA17_Chr1g0163181 37.255 204 105 8 15 205 1 194 1.10e-26 114
MsaG001430 MtrunA17_Chr1g0163181 40.777 103 57 2 457 555 21 123 3.40e-14 75.9
MsaG001430 MtrunA17_Chr1g0163091 45.665 173 83 7 540 712 3 164 2.97e-31 120
MsaG001430 MtrunA17_Chr1g0163241 42.958 142 70 5 33 165 23 162 3.13e-25 102
MsaG001430 MtrunA17_Chr1g0163241 36.000 125 79 1 457 580 23 147 8.05e-18 81.6
MsaG001430 MtrunA17_Chr1g0163191 46.296 108 52 2 26 127 14 121 4.19e-25 102
MsaG001430 MtrunA17_Chr1g0163191 43.056 72 40 1 457 527 21 92 8.07e-13 67.0
MsaG001430 MtrunA17_Chr5g0419201 32.500 200 105 7 2 185 5 190 1.52e-22 95.9
MsaG001430 MtrunA17_Chr4g0067431 31.276 243 140 10 12 234 10 245 3.78e-21 95.5
MsaG001430 MtrunA17_Chr4g0067431 24.709 344 193 16 446 775 19 310 7.23e-12 67.4
MsaG001430 MtrunA17_Chr7g0233531 28.839 267 133 8 456 667 1 265 2.09e-20 92.4
MsaG001430 MtrunA17_Chr7g0233531 34.848 132 76 4 32 160 1 125 9.46e-14 72.4
MsaG001430 MtrunA17_Chr3g0131891 38.760 129 72 3 11 132 13 141 5.73e-20 92.0
MsaG001430 MtrunA17_Chr3g0131891 27.331 311 156 9 418 667 4 305 8.70e-20 91.7
MsaG001430 MtrunA17_Chr3g0103151 35.211 142 87 3 15 153 10 149 1.31e-19 86.7
MsaG001430 MtrunA17_Chr7g0233571 32.632 190 115 5 9 194 2 182 6.67e-19 86.3
MsaG001430 MtrunA17_Chr7g0233571 45.570 79 40 2 456 531 25 103 2.00e-11 64.7
MsaG001430 MtrunA17_Chr6g0467351 42.718 103 55 3 14 112 12 114 1.14e-17 83.2
MsaG001430 MtrunA17_Chr1g0163061 44.660 103 53 2 14 112 12 114 2.64e-17 82.8
MsaG001430 MtrunA17_Chr3g0103191 31.548 168 101 4 7 162 2 167 6.78e-17 81.3
MsaG001430 MtrunA17_Chr1g0163221 39.623 106 60 2 11 112 5 110 1.30e-16 82.4
MsaG001430 MtrunA17_Chr3g0103181 37.594 133 76 5 7 135 2 131 2.57e-16 80.1
MsaG001430 MtrunA17_Chr7g0233561 32.121 165 97 4 10 165 11 169 3.87e-16 80.5
MsaG001430 MtrunA17_Chr3g0077451 26.695 236 137 7 457 667 12 236 1.17e-15 77.4
MsaG001430 MtrunA17_Chr3g0103171 33.613 119 76 2 33 148 6 124 1.47e-15 77.8
MsaG001430 MtrunA17_Chr5g0419211 39.130 92 55 1 585 676 11 101 4.59e-15 72.0
MsaG001430 MtrunA17_Chr3g0077411 26.506 249 132 10 457 664 278 516 1.72e-14 77.0
MsaG001430 MtrunA17_Chr3g0077411 22.455 334 221 13 457 775 41 351 6.03e-13 72.0
MsaG001430 MtrunA17_Chr3g0077411 31.897 116 75 2 1 112 246 361 1.41e-12 70.9
MsaG001430 MtrunA17_Chr7g0233541 30.412 194 121 5 32 216 1 189 7.00e-14 73.2
MsaG001430 MtrunA17_Chr7g0233541 39.394 99 57 2 456 551 1 99 6.96e-11 64.3
MsaG001430 MtrunA17_Chr8g0390841 29.221 154 101 5 15 160 37 190 1.17e-13 74.3
MsaG001430 MtrunA17_Chr1g0208771 32.609 138 85 4 33 162 13 150 1.60e-13 73.6
MsaG001430 MtrunA17_Chr7g0233521 43.182 88 47 2 32 116 1 88 2.99e-13 70.9
MsaG001430 MtrunA17_Chr3g0077461 29.487 156 92 4 16 168 236 376 1.13e-12 71.2
MsaG001430 MtrunA17_Chr1g0163071 37.037 108 64 3 32 135 1 108 3.68e-12 68.2
MsaG001430 MtrunA17_Chr5g0429931 26.908 249 127 12 457 666 11 243 4.35e-12 67.0
MsaG001430 MtrunA17_Chr3g0077361 24.060 266 174 10 457 705 56 310 2.91e-11 66.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG001430 AT2G21790.1 65.979 291 52 4 205 448 144 434 3.00e-123 389

Find 144 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAGACCCATTTGGAAGTTTA+AGG 0.149010 1:+20039274 None:intergenic
CGATAATTTGAGAGTGAAAT+TGG 0.170501 1:+20038583 None:intergenic
TTGGGTGCCTGATCTCTTTA+TGG 0.175397 1:-20040444 MsaT001430.1:CDS
TTTGGAAGTATAAGGGAAAT+TGG 0.230152 1:+20046852 None:intergenic
TGCAATGCGGTTATGATATT+TGG 0.243865 1:-20039233 MsaT001430.1:CDS
CAGTCCCATTTGGAAGTATA+AGG 0.247507 1:+20046844 None:intergenic
TTTGGAAGTTTAAGGGATAT+TGG 0.281020 1:+20039282 None:intergenic
ATTATCAAAATTATGTGTTC+AGG 0.281454 1:-20044593 MsaT001430.1:CDS
CATTCAACTTTGTGAATTGC+AGG 0.285867 1:-20048880 MsaT001430.1:CDS
TATATTGGATGATAGTAGTT+TGG 0.295324 1:-20046691 MsaT001430.1:CDS
GGGCAGGGTTCCTTTGTGGC+TGG 0.299989 1:+20038000 None:intergenic
TACGTGCCCATGGTTACATT+TGG 0.313598 1:-20040658 MsaT001430.1:CDS
TGGAAAGAGTTCAGAGCAAT+TGG 0.321369 1:-20040424 MsaT001430.1:CDS
ACGTGCCCATGGTTACATTT+GGG 0.323530 1:-20040657 MsaT001430.1:CDS
GAAAAGCAACCAGCAGAATC+TGG 0.327390 1:-20040201 MsaT001430.1:CDS
AGTTTAAGGGATATTGGATT+TGG 0.329165 1:+20039288 None:intergenic
AAAAGACATAATATATTTAG+TGG 0.334344 1:-20037383 MsaT001430.1:CDS
GGACTGAGTTCAAAATGAAT+TGG 0.337013 1:-20046827 MsaT001430.1:CDS
ATGAATGCAAAGTGAAGATT+AGG 0.340045 1:-20044641 MsaT001430.1:CDS
GGAAGGTGATAGATTAAAAT+TGG 0.340370 1:-20038549 MsaT001430.1:intron
TATAATGAACATCTCGACTA+TGG 0.355043 1:-20037308 MsaT001430.1:CDS
CTCTGGGCAGGGTTCCTTTG+TGG 0.360778 1:+20037996 None:intergenic
TTGAATTGGTCATGTAAATT+CGG 0.365554 1:+20037541 None:intergenic
ATTCACAAAGTTGAATGGTT+TGG 0.365673 1:+20048886 None:intergenic
TTGAAGTCACAGATAGAAAC+TGG 0.367181 1:-20040260 MsaT001430.1:CDS
TGCCAAACCAGGTGCTTCAT+TGG 0.367675 1:+20040382 None:intergenic
AATTCGGTGGAGTTGAAAAT+AGG 0.372119 1:-20037359 MsaT001430.1:CDS
GGCAGGGTTCCTTTGTGGCT+GGG 0.376943 1:+20038001 None:intergenic
CACCTGGTTTGGCAGATTGT+TGG 0.380255 1:-20040373 MsaT001430.1:CDS
AATCTGACCCTCCTTTGTAT+TGG 0.385935 1:+20046724 None:intergenic
CTGCAATTCACAAAGTTGAA+TGG 0.392583 1:+20048881 None:intergenic
TAATATATTTAGTGGAAATT+CGG 0.396970 1:-20037375 MsaT001430.1:CDS
AGACCCATTTGGAAGTTTAA+GGG 0.399549 1:+20039275 None:intergenic
GGTTACATTTGGGGAACAAA+TGG 0.400707 1:-20040647 MsaT001430.1:CDS
TCCCAATGAAGCACCTGGTT+TGG 0.407104 1:-20040384 MsaT001430.1:CDS
GTGCTTCATTGGGACAGAAC+AGG 0.422046 1:+20040393 None:intergenic
GAAATACCTTGAAACTGCTC+TGG 0.423422 1:+20037979 None:intergenic
ATTTCCCTTATACTTCCAAA+TGG 0.426494 1:-20046849 MsaT001430.1:CDS
GTGAAATTGGGATTCTCATT+GGG 0.428059 1:+20038596 None:intergenic
ATGATGTTTGGCTTCAGAAT+TGG 0.428724 1:-20046791 MsaT001430.1:CDS
GGAACAAATGGAACGTCTAA+TGG 0.432244 1:-20040635 MsaT001430.1:CDS
AGTGAAATTGGGATTCTCAT+TGG 0.432342 1:+20038595 None:intergenic
CATGTCATTATATTAGATGA+TGG 0.436157 1:-20039129 MsaT001430.1:intron
ACCTTGAAACTGCTCTGGGC+AGG 0.439680 1:+20037984 None:intergenic
TCTTATCTGTTGAAAGTTGA+AGG 0.443910 1:-20040962 MsaT001430.1:CDS
GAATTTACATGACCAATTCA+AGG 0.445932 1:-20037539 MsaT001430.1:CDS
GGTCTGTGTGCAAAATGAAT+TGG 0.448911 1:-20039257 MsaT001430.1:CDS
ATATCCCTTAAACTTCCAAA+TGG 0.449227 1:-20039279 MsaT001430.1:CDS
CTCAAACAAGCAAGTGTCTC+CGG 0.453496 1:+20037235 None:intergenic
GCCAAACCAGGTGCTTCATT+GGG 0.453941 1:+20040383 None:intergenic
AGTAAATCTGCTGGAGGGAT+TGG 0.455606 1:-20040698 MsaT001430.1:CDS
AAGTCATCAAATCTGTGCAC+TGG 0.456850 1:-20040170 MsaT001430.1:CDS
CAAATTATCGCGATCTTATG+TGG 0.458151 1:-20038570 MsaT001430.1:CDS
TGTCTCTCTACAAATTTCCT+TGG 0.462003 1:+20046894 None:intergenic
GACTGAGTTCAAAATGAATT+GGG 0.462417 1:-20046826 MsaT001430.1:CDS
TTATCAAAATTATGTGTTCA+GGG 0.463028 1:-20044592 MsaT001430.1:intron
GGAAATTTGTAGAGAGACAT+TGG 0.464411 1:-20046890 MsaT001430.1:CDS
GATAATTTGAGAGTGAAATT+GGG 0.465805 1:+20038584 None:intergenic
GGGGATTTATGATACATTGA+AGG 0.466284 1:-20040735 MsaT001430.1:CDS
ATAGTAATTGTGAATGAAAG+TGG 0.468831 1:+20038027 None:intergenic
GAAAGTTGAAGGAGTGGTTA+TGG 0.469081 1:-20040951 MsaT001430.1:CDS
AATAACTTTGAAATTACAGC+TGG 0.470091 1:-20038123 MsaT001430.1:CDS
AGTCCCATTTGGAAGTATAA+GGG 0.475942 1:+20046845 None:intergenic
CTAATAGAAACAATGGAGAC+AGG 0.476815 1:-20038685 MsaT001430.1:CDS
AATCTGACCCTCCTTTGTAC+TGG 0.482912 1:+20039154 None:intergenic
GACCTCAACATAAGATAATG+AGG 0.484092 1:-20040925 MsaT001430.1:CDS
TACAACGCAGTGATGATGTT+TGG 0.485691 1:-20046803 MsaT001430.1:CDS
TCAATGTCAAGTGTTCACTA+TGG 0.491973 1:-20048942 MsaT001430.1:CDS
TTTCCCTTATACTTCCAAAT+GGG 0.493674 1:-20046848 MsaT001430.1:CDS
ATGAAAGAGGATAGTATTGA+GGG 0.496212 1:-20040755 MsaT001430.1:intron
CCCTGCCCAGAGCAGTTTCA+AGG 0.500003 1:-20037985 MsaT001430.1:intron
TTCTGTCCCAATGAAGCACC+TGG 0.502866 1:-20040389 MsaT001430.1:CDS
TGAAAATAGGAGAAGGAACA+TGG 0.504948 1:-20037346 MsaT001430.1:CDS
TAATTGTGCCCAGATTCTGC+TGG 0.508224 1:+20040192 None:intergenic
GTCTGTGTGCAAAATGAATT+GGG 0.510202 1:-20039256 MsaT001430.1:CDS
TTGCCAATGTTTGTCCCTTG+AGG 0.510253 1:+20046507 None:intergenic
TATCTCTCTACAAAACTCCT+TGG 0.511924 1:+20039324 None:intergenic
GGAAAGAGTTCAGAGCAATT+GGG 0.512047 1:-20040423 MsaT001430.1:CDS
CGTGCCCATGGTTACATTTG+GGG 0.512128 1:-20040656 MsaT001430.1:CDS
CATGAAAGAGGATAGTATTG+AGG 0.513073 1:-20040756 MsaT001430.1:intron
ATCATTTCATGTCTCAGAGA+TGG 0.514754 1:-20040853 MsaT001430.1:CDS
AAGTATAAGGGAAATTGGAT+TGG 0.516318 1:+20046857 None:intergenic
ACTCTTTCCATAAAGAGATC+AGG 0.516899 1:+20040437 None:intergenic
AAGATAATGAGGGTTTCTGT+TGG 0.517292 1:-20040914 MsaT001430.1:CDS
TGAATGCAAAGTGAAGATTA+GGG 0.518516 1:-20044640 MsaT001430.1:CDS
TATCCCTTAAACTTCCAAAT+GGG 0.519149 1:-20039278 MsaT001430.1:CDS
CAACTACTCAACTGAGGCCT+AGG 0.520856 1:+20040791 None:intergenic
TGTGCAAAATCATGTGTTCA+AGG 0.521657 1:-20037204 MsaT001430.1:CDS
TGATTGAACACACGCAGCAT+TGG 0.527196 1:+20040605 None:intergenic
AAGGAACATGACTTGCAAGT+TGG 0.533438 1:-20038084 MsaT001430.1:CDS
GACTATGGATGGTTCACTAA+AGG 0.536976 1:-20037293 MsaT001430.1:CDS
AAAAGCAACCAGCAGAATCT+GGG 0.538023 1:-20040200 MsaT001430.1:CDS
CAATGATGAACAAGAAGCTA+AGG 0.539148 1:-20046643 MsaT001430.1:CDS
TGGACGTGGAAGTGAAGCTA+AGG 0.540987 1:-20048922 MsaT001430.1:CDS
TATATTTAGTGGAAATTCGG+TGG 0.544355 1:-20037372 MsaT001430.1:CDS
ACTTTGAAATTACAGCTGGT+TGG 0.547063 1:-20038119 MsaT001430.1:CDS
ACTAGTGCTGCTACACCTCA+AGG 0.555707 1:-20046522 MsaT001430.1:CDS
AATGCAATGATTAGAAGCAC+TGG 0.557729 1:-20038192 MsaT001430.1:CDS
TGAATTGGAAAAGATTTGCA+CGG 0.558326 1:-20039206 MsaT001430.1:CDS
ATGGATGGTTCACTAAAGGC+TGG 0.558556 1:-20037289 MsaT001430.1:CDS
GCTGTCATTAGTAAATCTGC+TGG 0.558625 1:-20040707 MsaT001430.1:CDS
GAGTTCAGAGCAATTGGGAA+TGG 0.558649 1:-20040418 MsaT001430.1:CDS
TTGTTCCCCAAATGTAACCA+TGG 0.559225 1:+20040651 None:intergenic
TTTGGCTTCAGAATTGGAAG+AGG 0.559399 1:-20046785 MsaT001430.1:CDS
CCTTGAAACTGCTCTGGGCA+GGG 0.560907 1:+20037985 None:intergenic
ATCCAACAATCTGCCAAACC+AGG 0.561932 1:+20040371 None:intergenic
AAAGAGATCAGGCACCCAAA+GGG 0.564159 1:+20040448 None:intergenic
TACATGAGTGAGCTGTTGCA+AGG 0.564294 1:-20038216 MsaT001430.1:CDS
ATGAACATCTCGACTATGGA+TGG 0.566956 1:-20037304 MsaT001430.1:CDS
TCTGTTGAAAGTTGAAGGAG+TGG 0.570360 1:-20040957 MsaT001430.1:CDS
ACACCTCAAGGGACAAACAT+TGG 0.575666 1:-20046510 MsaT001430.1:CDS
AGAGATTGCAAAGTAAAGAC+CGG 0.577584 1:-20037254 MsaT001430.1:CDS
GCAGAGGCTAATAGAAACAA+TGG 0.580274 1:-20038692 MsaT001430.1:intron
TGCTTCATTGGGACAGAACA+GGG 0.584427 1:+20040394 None:intergenic
TTAGAAGCACTGGTGAGGAC+AGG 0.585346 1:-20038182 MsaT001430.1:CDS
GTTCACAACATACGTGCCCA+TGG 0.585675 1:-20040668 MsaT001430.1:CDS
TTTGCGCGCTCTCTCAGATG+TGG 0.586381 1:-20046762 MsaT001430.1:CDS
TGTGCTTGCAGATGATTCCA+AGG 0.587783 1:-20046911 MsaT001430.1:intron
TGTTCCCCAAATGTAACCAT+GGG 0.588262 1:+20040652 None:intergenic
AGTCATCAAATCTGTGCACT+GGG 0.588347 1:-20040169 MsaT001430.1:CDS
AAATACCTTGAAACTGCTCT+GGG 0.593332 1:+20037980 None:intergenic
TTATTCAAGCACGGAGTCTG+TGG 0.593607 1:-20040292 MsaT001430.1:CDS
TTTGCACGGTCGCTTAGATG+CGG 0.593941 1:-20039192 MsaT001430.1:CDS
ACCCTCATTATCTTATGTTG+AGG 0.594820 1:+20040923 None:intergenic
TTATCGCGATCTTATGTGGA+AGG 0.597524 1:-20038566 MsaT001430.1:CDS
TAAAGAGATCAGGCACCCAA+AGG 0.610062 1:+20040447 None:intergenic
TCAAGTGTTCACTATGGACG+TGG 0.612848 1:-20048936 MsaT001430.1:CDS
ACATGAGTGAGCTGTTGCAA+GGG 0.614327 1:-20038215 MsaT001430.1:CDS
GTCATTAGTAAATCTGCTGG+AGG 0.617415 1:-20040704 MsaT001430.1:CDS
GGTTTCTGTTGGAATTCACA+AGG 0.619618 1:-20040903 MsaT001430.1:CDS
GCATGTTATGTTGATCAGAA+AGG 0.634303 1:-20040578 MsaT001430.1:intron
GGACGTGGAAGTGAAGCTAA+GGG 0.634918 1:-20048921 MsaT001430.1:CDS
GCACTGGTGAGGACAGGACG+TGG 0.639691 1:-20038176 MsaT001430.1:CDS
ACCTCAACATAAGATAATGA+GGG 0.640474 1:-20040924 MsaT001430.1:CDS
TAATAGAAACAATGGAGACA+GGG 0.645437 1:-20038684 MsaT001430.1:CDS
TACTATTATCCCAGCCACAA+AGG 0.646969 1:-20038010 MsaT001430.1:CDS
GTGGAGTTGAAAATAGGAGA+AGG 0.647534 1:-20037353 MsaT001430.1:CDS
GTTCCAGCGTTGAAAAGTGT+AGG 0.657151 1:+20040815 None:intergenic
CTAGTGCTGCTACACCTCAA+GGG 0.659036 1:-20046521 MsaT001430.1:CDS
AATGATTAGAAGCACTGGTG+AGG 0.660410 1:-20038187 MsaT001430.1:CDS
TCATTAGTAAATCTGCTGGA+GGG 0.679752 1:-20040703 MsaT001430.1:CDS
TGAAAGAGGATAGTATTGAG+GGG 0.695012 1:-20040754 MsaT001430.1:intron
AAAATGAATTGGGTGCAATG+CGG 0.797500 1:-20039246 MsaT001430.1:CDS
TGAAACAGAGGATACCCACG+TGG 0.823355 1:-20038251 MsaT001430.1:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr1 gene 20037190 20048961 20037190 ID=MsaG001430
Chr1 mRNA 20037190 20048961 20037190 ID=MsaT001430.1;Parent=MsaG001430
Chr1 exon 20037190 20037432 20037190 ID=MsaT001430.1.exon12;Parent=MsaT001430.1
Chr1 CDS 20037190 20037432 20037190 ID=cds.MsaT001430.1;Parent=MsaT001430.1
Chr1 exon 20037516 20037574 20037516 ID=MsaT001430.1.exon11;Parent=MsaT001430.1
Chr1 CDS 20037516 20037574 20037516 ID=cds.MsaT001430.1;Parent=MsaT001430.1
Chr1 exon 20037986 20038265 20037986 ID=MsaT001430.1.exon10;Parent=MsaT001430.1
Chr1 CDS 20037986 20038265 20037986 ID=cds.MsaT001430.1;Parent=MsaT001430.1
Chr1 exon 20038550 20038710 20038550 ID=MsaT001430.1.exon9;Parent=MsaT001430.1
Chr1 CDS 20038550 20038710 20038550 ID=cds.MsaT001430.1;Parent=MsaT001430.1
Chr1 exon 20039130 20039352 20039130 ID=MsaT001430.1.exon8;Parent=MsaT001430.1
Chr1 CDS 20039130 20039352 20039130 ID=cds.MsaT001430.1;Parent=MsaT001430.1
Chr1 exon 20039732 20039753 20039732 ID=MsaT001430.1.exon7;Parent=MsaT001430.1
Chr1 CDS 20039732 20039753 20039732 ID=cds.MsaT001430.1;Parent=MsaT001430.1
Chr1 exon 20040161 20040497 20040161 ID=MsaT001430.1.exon6;Parent=MsaT001430.1
Chr1 CDS 20040161 20040497 20040161 ID=cds.MsaT001430.1;Parent=MsaT001430.1
Chr1 exon 20040579 20040763 20040579 ID=MsaT001430.1.exon5;Parent=MsaT001430.1
Chr1 CDS 20040579 20040763 20040579 ID=cds.MsaT001430.1;Parent=MsaT001430.1
Chr1 exon 20040803 20040987 20040803 ID=MsaT001430.1.exon4;Parent=MsaT001430.1
Chr1 CDS 20040803 20040987 20040803 ID=cds.MsaT001430.1;Parent=MsaT001430.1
Chr1 exon 20044593 20044674 20044593 ID=MsaT001430.1.exon3;Parent=MsaT001430.1
Chr1 CDS 20044593 20044674 20044593 ID=cds.MsaT001430.1;Parent=MsaT001430.1
Chr1 exon 20046462 20046922 20046462 ID=MsaT001430.1.exon2;Parent=MsaT001430.1
Chr1 CDS 20046462 20046922 20046462 ID=cds.MsaT001430.1;Parent=MsaT001430.1
Chr1 exon 20048869 20048961 20048869 ID=MsaT001430.1.exon1;Parent=MsaT001430.1
Chr1 CDS 20048869 20048961 20048869 ID=cds.MsaT001430.1;Parent=MsaT001430.1
Gene Sequence

>MsaT001430.1

ATGTCAAGTGTTCACTATGGACGTGGAAGTGAAGCTAAGGGAATGCATTTTTACCAAACCATTCAACTTTGTGAATTGCAGGATGAAGAGCTTATGATTCCAAGGAAATTTGTAGAGAGACATTGGAAAGATGTATCCAATCCAATTTCCCTTATACTTCCAAATGGGACTGAGTTCAAAATGAATTGGGTACAACGCAGTGATGATGTTTGGCTTCAGAATTGGAAGAGGTTTGCGCGCTCTCTCAGATGTGGAGATCTTTTAGTTTTCCAATACAAAGGAGGGTCAGATTTTCATGTCATTATATTGGATGATAGTAGTTTGGAAATTGACTATTCATGTATGAGATGCAATGATGAACAAGAAGCTAAGGAGAGTGATGATGATGATGACTGTGTTGAGATTCCGAATGATCATGAAAATACCCAACAACCCCTCAAGAAAAAGAAAATCAACTCAAATGACATTGCTACTAGTGCTGCTACACCTCAAGGGACAAACATTGGCAAGAGAAAAATTAATATCAATGCATCCTTGCAAAAAGTTTCAGGCAATTCATCTGTGAATGAATGCAAAGTGAAGATTAGGGACACTAGTTTGTATGAGATGATTGATTATCAAAATTATGTGTTCAGGAGGTCTTATCTGTTGAAAGTTGAAGGAGTGGTTATGGAAAGACCTCAACATAAGATAATGAGGGTTTCTGTTGGAATTCACAAGGATGATGTTGAATCTGCTGTCAAAACTTATCATTTCATGTCTCAGAGATGGTTCACTCATGCTTCTCCTACACTTTTCAACGCTGGAACACCTAGGCCTCATATTGAGGGGATTTATGATACATTGAAGGAGTGTGCTGTCATTAGTAAATCTGCTGGAGGGATTGGTGTTTCTGTTCACAACATACGTGCCCATGGTTACATTTGGGGAACAAATGGAACGTCTAATGGTATTGTTCCAATGCTGCGTGTGTTCAATCATACTGCATGTTATGTTGATCAGAAAGAGCAATGTGCTCGAGATTTATTTTACGCCCTTTGGGTGCCTGATCTCTTTATGGAAAGAGTTCAGAGCAATTGGGAATGGTCCCTGTTCTGTCCCAATGAAGCACCTGGTTTGGCAGATTGTTGGATTCAAGAATATGATAAGCTGTACGCTCAGTATGAAAAAGCAGGAAAAGCAAAAAAGATTATTCAAGCACGGAGTCTGTGGTTTGCAGTTTTGAAGTCACAGATAGAAACTGGAAATCCTTACATGCTTTTTAAGGATGATTGCAATAGGAAAAGCAACCAGCAGAATCTGGGCACAATTAAGTCATCAAATCTGTGCACTGGGATAATCGAAACTAATTTCAGAGCAGCTCCTATGATTCCAAGGAGTTTTGTAGAGAGATATTGGAAAGATGTGCCAAATCCAATATCCCTTAAACTTCCAAATGGGTCTGTGTGCAAAATGAATTGGGTGCAATGCGGTTATGATATTTGGCTTTTGAATTGGAAAAGATTTGCACGGTCGCTTAGATGCGGTGATCTTTTAGTTTTCCAGTACAAAGGAGGGTCAGATTTTCATGTCATTATATTAGATGATGAGGCTAATAGAAACAATGGAGACAGGGATAAGAAAGCTAAGAAATGCACAAAAACTAAAGATGGGAATGAGAGAAGTTACACAAATTCTCATCCCAATGAGAATCCCAATTTCACTCTCAAATTATCGCGATCTTATGTGGAAGGTGATAGATTAAAATTGAGGATACCCACGTGGTTTTCAAAAGAATACATGAGTGAGCTGTTGCAAGGGAATGCAATGATTAGAAGCACTGGTGAGGACAGGACGTGGTATGTGACGTTAAAATTTGATGAACGCAATAATAACTTTGAAATTACAGCTGGTTGGAAGTCATTTTTAAAGGAACATGACTTGCAAGTTGGTGATGTATGTAAATTTGAGATGACTCAACGCGAACCACTTTCATTCACAATTACTATTATCCCAGCCACAAAGGAACCCTGCCCAGAGCAGTTTCAAGATTCTGTAAGTCCGAATTTACATGACCAATTCAAGGTTTTTGTAACATCATTAGACAAGTTGGTTGTACTACCAAGTGAGCTGTTAAAAAGACATAATATATTTAGTGGAAATTCGGTGGAGTTGAAAATAGGAGAAGGAACATGGTTTGTAGATGTGAGTTATAATGAACATCTCGACTATGGATGGTTCACTAAAGGCTGGCAAGAATTTGTGAGAGATTGCAAAGTAAAGACCGGAGACACTTGCTTGTTTGAGATGATTGATGTGCAAAATCATGTGTTCAAGGTTTCAATTGTTTGA
Protein sequence

>MsaT001430.1

MSSVHYGRGSEAKGMHFYQTIQLCELQDEELMIPRKFVERHWKDVSNPISLILPNGTEFKMNWVQRSDDVWLQNWKRFARSLRCGDLLVFQYKGGSDFHVIILDDSSLEIDYSCMRCNDEQEAKESDDDDDCVEIPNDHENTQQPLKKKKINSNDIATSAATPQGTNIGKRKININASLQKVSGNSSVNECKVKIRDTSLYEMIDYQNYVFRRSYLLKVEGVVMERPQHKIMRVSVGIHKDDVESAVKTYHFMSQRWFTHASPTLFNAGTPRPHIEGIYDTLKECAVISKSAGGIGVSVHNIRAHGYIWGTNGTSNGIVPMLRVFNHTACYVDQKEQCARDLFYALWVPDLFMERVQSNWEWSLFCPNEAPGLADCWIQEYDKLYAQYEKAGKAKKIIQARSLWFAVLKSQIETGNPYMLFKDDCNRKSNQQNLGTIKSSNLCTGIIETNFRAAPMIPRSFVERYWKDVPNPISLKLPNGSVCKMNWVQCGYDIWLLNWKRFARSLRCGDLLVFQYKGGSDFHVIILDDEANRNNGDRDKKAKKCTKTKDGNERSYTNSHPNENPNFTLKLSRSYVEGDRLKLRIPTWFSKEYMSELLQGNAMIRSTGEDRTWYVTLKFDERNNNFEITAGWKSFLKEHDLQVGDVCKFEMTQREPLSFTITIIPATKEPCPEQFQDSVSPNLHDQFKVFVTSLDKLVVLPSELLKRHNIFSGNSVELKIGEGTWFVDVSYNEHLDYGWFTKGWQEFVRDCKVKTGDTCLFEMIDVQNHVFKVSIV*