AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG001432


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG001432 MtrunA17_Chr1g0163181 65.657 297 93 6 1 293 1 292 4.14e-127 369
MsaG001432 MtrunA17_Chr1g0163181 80.000 75 15 0 219 293 355 429 4.76e-34 128
MsaG001432 MtrunA17_Chr1g0163251 51.163 301 117 4 1 293 1 279 6.38e-98 293
MsaG001432 MtrunA17_Chr1g0163201 59.155 284 89 6 20 293 18 284 1.70e-95 287
MsaG001432 MtrunA17_Chr1g0163201 66.667 105 30 1 189 293 293 392 3.76e-38 139
MsaG001432 MtrunA17_Chr1g0163191 73.373 169 37 2 1 169 1 161 1.58e-75 228
MsaG001432 MtrunA17_Chr1g0163261 37.864 309 149 6 1 293 1 282 1.70e-64 204
MsaG001432 MtrunA17_Chr1g0163241 60.335 179 47 4 1 178 7 162 2.30e-62 194
MsaG001432 MtrunA17_Chr1g0163231 38.889 306 146 9 1 293 1 278 8.39e-62 197
MsaG001432 MtrunA17_Chr1g0171611 38.889 306 134 8 1 293 1 266 2.15e-59 191
MsaG001432 MtrunA17_Chr1g0163131 41.438 292 147 9 14 293 27 306 2.79e-57 186
MsaG001432 MtrunA17_Chr1g0163111 81.818 77 14 0 217 293 152 228 9.36e-38 133
MsaG001432 MtrunA17_Chr1g0163111 42.478 113 59 3 184 293 1 110 7.96e-18 80.5
MsaG001432 MtrunA17_Chr1g0163151 77.333 75 17 0 219 293 147 221 3.04e-36 129
MsaG001432 MtrunA17_Chr1g0163151 43.363 113 61 2 184 293 1 113 2.42e-22 92.8
MsaG001432 MtrunA17_Chr3g0103181 28.720 289 179 8 9 292 12 278 2.87e-33 123
MsaG001432 MtrunA17_Chr3g0103171 29.137 278 174 8 20 292 5 264 6.63e-32 119
MsaG001432 MtrunA17_Chr3g0131891 31.849 292 168 9 14 292 28 301 1.64e-30 117
MsaG001432 MtrunA17_Chr1g0163101 39.394 165 80 4 14 178 26 170 3.32e-30 112
MsaG001432 MtrunA17_Chr7g0233561 29.836 305 183 10 8 292 17 310 9.39e-30 115
MsaG001432 MtrunA17_Chr8g0390841 28.571 294 183 9 14 292 48 329 3.45e-29 116
MsaG001432 MtrunA17_Chr7g0233541 29.553 291 172 8 21 292 2 278 1.13e-28 111
MsaG001432 MtrunA17_Chr7g0233521 28.470 281 171 7 21 292 2 261 6.98e-28 108
MsaG001432 MtrunA17_Chr1g0162161 37.975 158 80 6 21 178 4 143 6.15e-27 103
MsaG001432 MtrunA17_Chr3g0103191 29.070 258 159 7 9 260 12 251 3.70e-26 103
MsaG001432 MtrunA17_Chr1g0208771 27.835 291 178 11 20 292 12 288 4.63e-26 107
MsaG001432 MtrunA17_Chr1g0163121 45.614 114 57 2 19 132 9 117 5.05e-26 100
MsaG001432 MtrunA17_Chr6g0476181 38.037 163 85 5 21 178 2 153 9.22e-26 100
MsaG001432 MtrunA17_Chr1g0163081 41.071 112 61 2 21 132 6 112 7.13e-24 94.7
MsaG001432 MtrunA17_Chr7g0233571 31.707 246 107 8 1 214 1 217 1.52e-22 93.2
MsaG001432 MtrunA17_Chr7g0233531 28.723 282 169 8 21 292 2 261 2.82e-22 94.0
MsaG001432 MtrunA17_Chr3g0103151 32.692 156 88 4 9 158 12 156 8.22e-22 89.7
MsaG001432 MtrunA17_Chr1g0163141 32.544 169 101 4 17 178 7 169 4.93e-21 88.2
MsaG001432 MtrunA17_Chr1g0163071 27.869 305 166 10 21 292 2 285 1.75e-20 89.4
MsaG001432 MtrunA17_Chr1g0163221 29.286 280 162 9 14 280 20 276 4.20e-20 89.0
MsaG001432 MtrunA17_Chr1g0163161 42.000 100 54 3 33 132 1 96 2.04e-19 82.4
MsaG001432 MtrunA17_Chr4g0067431 28.276 290 183 10 9 280 15 297 2.09e-19 86.7
MsaG001432 MtrunA17_Chr5g0419201 32.653 147 90 3 14 158 25 164 8.95e-19 82.4
MsaG001432 MtrunA17_Chr6g0467351 36.697 109 66 2 13 118 23 131 1.69e-16 77.0
MsaG001432 MtrunA17_Chr3g0103201 25.862 232 152 6 64 292 5 219 3.24e-14 70.5
MsaG001432 MtrunA17_Chr4g0033961 37.624 101 57 2 7 101 4 104 1.06e-13 70.9
MsaG001432 MtrunA17_Chr6g0476171 50.820 61 30 0 233 293 185 245 2.26e-13 68.6
MsaG001432 MtrunA17_Chr1g0162441 33.333 111 67 3 7 110 5 115 2.96e-12 66.6
MsaG001432 MtrunA17_Chr1g0163091 31.624 117 72 3 184 292 1 117 9.88e-12 62.4
MsaG001432 MtrunA17_Chr3g0077461 21.111 270 151 7 14 281 246 455 1.20e-11 64.7
MsaG001432 MtrunA17_Chr1g0163061 36.264 91 55 2 14 101 24 114 1.66e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG001432 AT3G18990.1 28.313 332 189 15 7 291 4 333 6.10e-24 99.8
MsaG001432 AT3G18990.2 28.313 332 189 15 7 291 4 333 8.06e-24 100

Find 47 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAACACCGTTTGGAAATTTA+AGG 0.151014 1:+20061949 None:intergenic
TAGAGGTTGGATAAAATTTA+TGG 0.178729 1:-20059667 MsaT001432.1:CDS
TTTGCAAAGTCTTTCAAATA+TGG 0.208675 1:-20061864 MsaT001432.1:CDS
GCCAAAGGAGTTTATGAAAA+TGG 0.215569 1:-20059784 MsaT001432.1:CDS
AAATATTGTGGAGTTGAAAA+TGG 0.256652 1:-20059751 MsaT001432.1:CDS
TTCATCAACTGATTTGATAT+TGG 0.273749 1:+20061758 None:intergenic
TATTTCTCCACATATATCTT+AGG 0.281171 1:+20061999 None:intergenic
TGGAGAGTAATGGTGATATT+TGG 0.287546 1:-20061908 MsaT001432.1:CDS
TTATGCCAATGGCTATTTCT+TGG 0.296975 1:-20061090 MsaT001432.1:intron
TTTGGAAATTTAAGGAATAT+AGG 0.302976 1:+20061957 None:intergenic
CCAACTAAAATGATAATAAA+TGG 0.306043 1:-20061603 MsaT001432.1:CDS
CTCTTAACTTTCAAATTCAT+TGG 0.314459 1:-20061837 MsaT001432.1:CDS
CATTAACAGCTTCAGATGTT+AGG 0.318603 1:+20061147 None:intergenic
AGATATATGTGGAGAAATAT+TGG 0.326941 1:-20061995 MsaT001432.1:CDS
TTGGAAATTTAAGGAATATA+GGG 0.346499 1:+20061958 None:intergenic
TGGCAAGAGAAAATTGAGTA+TGG 0.387713 1:-20061583 MsaT001432.1:CDS
ATAATTTATTTCTAAAGTAT+TGG 0.398912 1:+20061782 None:intergenic
CATGTCATTTCAATATGTTC+CGG 0.405450 1:-20062186 MsaT001432.1:CDS
CCAAAGGAGTTTATGAAAAT+GGG 0.408096 1:-20059783 MsaT001432.1:CDS
GAAGAATGCAAAGTGGAGAT+TGG 0.433387 1:-20059645 MsaT001432.1:CDS
AATATTGTGGAGTTGAAAAT+GGG 0.451536 1:-20059750 MsaT001432.1:CDS
AAGATTATCCTATGAAAATC+CGG 0.460649 1:+20062167 None:intergenic
CAAAGTAGCCATGGTGAAGA+AGG 0.465707 1:-20061180 MsaT001432.1:CDS
TTAACTTTCAAATTCATTGG+TGG 0.475675 1:-20061834 MsaT001432.1:CDS
CGTGGCTGTAGGTTCGGTAG+AGG 0.492240 1:-20059684 MsaT001432.1:CDS
CTCTTCATGACTTTCTTCTG+TGG 0.498976 1:+20061674 None:intergenic
AACTATTACGAGAGTATTCG+TGG 0.500607 1:-20059702 MsaT001432.1:CDS
AGTATTCGTGGCTGTAGGTT+CGG 0.502330 1:-20059690 MsaT001432.1:CDS
AGTCATTATAACTTCAAAAG+AGG 0.507233 1:+20061118 None:intergenic
ACCACCCAACCAAAATTCTC+AGG 0.510316 1:-20061552 MsaT001432.1:intron
GGGATATCATGAAAATATTG+TGG 0.512948 1:-20059763 MsaT001432.1:CDS
GTCATTATAACTTCAAAAGA+GGG 0.514498 1:+20061119 None:intergenic
GCTGTAGGTTCGGTAGAGGT+TGG 0.515331 1:-20059680 MsaT001432.1:CDS
ATATTCCTTAAATTTCCAAA+CGG 0.536219 1:-20061954 MsaT001432.1:CDS
AATATCAAATCAGTTGATGA+AGG 0.540855 1:-20061756 MsaT001432.1:CDS
TGATGACAGTTCAGATGAGG+TGG 0.583984 1:-20061637 MsaT001432.1:CDS
GAGGGTTCCTAAGATATATG+TGG 0.586451 1:-20062006 MsaT001432.1:CDS
ATGACTCGATCTTATGCCAA+TGG 0.587654 1:-20061101 MsaT001432.1:CDS
AGGTAGTAACAAAGTAGCCA+TGG 0.603454 1:-20061189 MsaT001432.1:CDS
GTATCTGCAGTGTGTGCCAA+AGG 0.603540 1:-20059799 MsaT001432.1:intron
ACTTACCAAGAAATAGCCAT+TGG 0.604417 1:+20061085 None:intergenic
TATGTGGAGAAATATTGGAA+AGG 0.617787 1:-20061990 MsaT001432.1:CDS
ACGAGAGTATTCGTGGCTGT+AGG 0.618825 1:-20059695 MsaT001432.1:CDS
ATTTATGGAAGAATGCAAAG+TGG 0.635124 1:-20059652 MsaT001432.1:CDS
TGAAAATGGGGAGAAAGTCA+TGG 0.640455 1:-20059737 MsaT001432.1:CDS
AAGTGATGACAGTTCAGATG+AGG 0.649994 1:-20061640 MsaT001432.1:CDS
ATATTGTGGAGTTGAAAATG+GGG 0.692849 1:-20059749 MsaT001432.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr1 gene 20059581 20062207 20059581 ID=MsaG001432
Chr1 mRNA 20059581 20062207 20059581 ID=MsaT001432.1;Parent=MsaG001432
Chr1 exon 20059581 20059811 20059581 ID=MsaT001432.1.exon4;Parent=MsaT001432.1
Chr1 CDS 20059581 20059811 20059581 ID=cds.MsaT001432.1;Parent=MsaT001432.1
Chr1 exon 20061091 20061209 20061091 ID=MsaT001432.1.exon3;Parent=MsaT001432.1
Chr1 CDS 20061091 20061209 20061091 ID=cds.MsaT001432.1;Parent=MsaT001432.1
Chr1 exon 20061553 20062027 20061553 ID=MsaT001432.1.exon2;Parent=MsaT001432.1
Chr1 CDS 20061553 20062027 20061553 ID=cds.MsaT001432.1;Parent=MsaT001432.1
Chr1 exon 20062151 20062207 20062151 ID=MsaT001432.1.exon1;Parent=MsaT001432.1
Chr1 CDS 20062151 20062207 20062151 ID=cds.MsaT001432.1;Parent=MsaT001432.1
Gene Sequence

>MsaT001432.1

ATGTCATTTCAATATGTTCCGGATTTTCATAGGATAATCTTGCAAGATAAGAAACTTAGGGTTCCTAAGATATATGTGGAGAAATATTGGAAAGGAATATCAAACCCTATATTCCTTAAATTTCCAAACGGTGTTGAACAAAAAATATTTTGGGTGGAGAGTAATGGTGATATTTGGTTTCAAAAAAATTGGGAAAGTTTTGCAAAGTCTTTCAAATATGGATATCTCTTAACTTTCAAATTCATTGGTGGATCATATTTTAAGGTTAAGATATTTGGTGCCAATACTTTAGAAATAAATTATGCCAATATCAAATCAGTTGATGAAGGTGCTGAAGACACAGAAGAAGAAAGTCATGAAGAGAGTGATGAATCAAGTGATGATGAAGCCACAGAAGAAAGTCATGAAGAGAGTGATGAATCAAGTGATGACAGTTCAGATGAGGTGGAAATGCCAAAGCCAACTAAAATGATAATAAATGGCAAGAGAAAATTGAGTATGGATTTTGACACCACCCAACCAAAATTCTCAGGTAGTAACAAAGTAGCCATGGTGAAGAAGGCTAGAAAATACCTAACATCTGAAGCTGTTAATGAGAATCCCTCTTTTGAAGTTATAATGACTCGATCTTATGCCAATGGCTATTTCTTGTGTGTGCCAAAGGAGTTTATGAAAATGGGATATCATGAAAATATTGTGGAGTTGAAAATGGGGAGAAAGTCATGGCTCGTAAAAGTCAACTATTACGAGAGTATTCGTGGCTGTAGGTTCGGTAGAGGTTGGATAAAATTTATGGAAGAATGCAAAGTGGAGATTGGAGATACTTGTCTCTTCAAGTTAATTGATGAGAGAAAATTTGTGTTTGATGTTTCAATTGTTTGA

Protein sequence

>MsaT001432.1

MSFQYVPDFHRIILQDKKLRVPKIYVEKYWKGISNPIFLKFPNGVEQKIFWVESNGDIWFQKNWESFAKSFKYGYLLTFKFIGGSYFKVKIFGANTLEINYANIKSVDEGAEDTEEESHEESDESSDDEATEESHEESDESSDDSSDEVEMPKPTKMIINGKRKLSMDFDTTQPKFSGSNKVAMVKKARKYLTSEAVNENPSFEVIMTRSYANGYFLCVPKEFMKMGYHENIVELKMGRKSWLVKVNYYESIRGCRFGRGWIKFMEECKVEIGDTCLFKLIDERKFVFDVSIV*