Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG001448 | XP_039688408.1 | 93.443 | 122 | 8 | 0 | 1 | 122 | 1 | 122 | 2.60e-78 | 236 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG001448 | tr|A0A072VG92|A0A072VG92_MEDTR | 93.443 | 122 | 8 | 0 | 1 | 122 | 262 | 383 | 5.72e-77 | 241 |
| Gene ID | Type | Classification |
|---|---|---|
| MsaG001448 | TF | B3 |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG001448 | MtrunA17_Chr6g0476171 | 76.471 | 102 | 23 | 1 | 21 | 122 | 145 | 245 | 1.06e-52 | 166 |
| MsaG001448 | MtrunA17_Chr1g0163151 | 40.367 | 109 | 58 | 3 | 14 | 122 | 120 | 221 | 4.28e-19 | 79.0 |
| MsaG001448 | MtrunA17_Chr1g0163111 | 47.561 | 82 | 39 | 3 | 42 | 122 | 150 | 228 | 8.95e-19 | 78.6 |
| MsaG001448 | MtrunA17_Chr1g0163131 | 45.455 | 88 | 46 | 1 | 35 | 122 | 221 | 306 | 2.19e-18 | 78.6 |
| MsaG001448 | MtrunA17_Chr1g0163201 | 44.444 | 90 | 46 | 2 | 33 | 122 | 307 | 392 | 6.49e-17 | 75.1 |
| MsaG001448 | MtrunA17_Chr1g0163181 | 38.938 | 113 | 62 | 3 | 10 | 122 | 324 | 429 | 1.95e-16 | 73.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 23 sgRNAs with CRISPR-Local
Find sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATGGAATATGCGGGCAATTT+TGG | 0.164513 | 1:+20245214 | MsaT001448.1:CDS |
| AAGAAGCATGGTATATTTAG+TGG | 0.374789 | 1:+20245541 | MsaT001448.1:CDS |
| TATAATGAACATCTCGACTA+TGG | 0.388378 | 1:+20245616 | MsaT001448.1:CDS |
| TGCTTCTTCAACAGCTCACT+TGG | 0.461685 | 1:-20245526 | None:intergenic |
| GAATTTACATGAGATGTTCA+AGG | 0.464486 | 1:+20245385 | MsaT001448.1:CDS |
| GACTATGGTTGGTTCACTAA+AGG | 0.474605 | 1:+20245631 | MsaT001448.1:CDS |
| TGAGAGTAGGGGAAGGAACA+TGG | 0.483751 | 1:+20245578 | MsaT001448.1:CDS |
| TGTGAGAGATTGCAAAGTAA+AGG | 0.489991 | 1:+20245666 | MsaT001448.1:CDS |
| TGTGCAAAATCATGTGTTCA+AGG | 0.521657 | 1:+20245720 | MsaT001448.1:CDS |
| AGAGATTGCAAAGTAAAGGC+TGG | 0.538378 | 1:+20245670 | MsaT001448.1:CDS |
| ATGAACATCTCGACTATGGT+TGG | 0.544359 | 1:+20245620 | MsaT001448.1:CDS |
| ATTCCGTGGAGTTGAGAGTA+GGG | 0.547073 | 1:+20245566 | MsaT001448.1:CDS |
| TATATTTAGTGGAAATTCCG+TGG | 0.570238 | 1:+20245552 | MsaT001448.1:CDS |
| ATGGTTGGTTCACTAAAGGT+TGG | 0.573713 | 1:+20245635 | MsaT001448.1:CDS |
| GTAAAAGATATGGAATATGC+GGG | 0.574921 | 1:+20245205 | None:intergenic |
| AATTCCGTGGAGTTGAGAGT+AGG | 0.585424 | 1:+20245565 | MsaT001448.1:CDS |
| CGTAAAAGATATGGAATATG+CGG | 0.600766 | 1:+20245204 | None:intergenic |
| AGTGAGCTGTTGAAGAAGCA+TGG | 0.617628 | 1:+20245529 | MsaT001448.1:CDS |
| TTCCCCTACTCTCAACTCCA+CGG | 0.627106 | 1:-20245569 | None:intergenic |
| GGAAGGAACATGGTTTGTAG+AGG | 0.629163 | 1:+20245588 | MsaT001448.1:CDS |
| TTCCGTGGAGTTGAGAGTAG+GGG | 0.660437 | 1:+20245567 | MsaT001448.1:CDS |
| GTGGAGTTGAGAGTAGGGGA+AGG | 0.685462 | 1:+20245571 | MsaT001448.1:CDS |
| TCTTATAACATCATCAGACA+AGG | 0.698207 | 1:+20245409 | MsaT001448.1:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr1 | gene | 20245214 | 20245756 | 20245214 | ID=MsaG001448 |
| Chr1 | mRNA | 20245214 | 20245756 | 20245214 | ID=MsaT001448.1;Parent=MsaG001448 |
| Chr1 | exon | 20245214 | 20245280 | 20245214 | ID=MsaT001448.1.exon1;Parent=MsaT001448.1 |
| Chr1 | CDS | 20245214 | 20245280 | 20245214 | ID=cds.MsaT001448.1;Parent=MsaT001448.1 |
| Chr1 | exon | 20245372 | 20245430 | 20245372 | ID=MsaT001448.1.exon2;Parent=MsaT001448.1 |
| Chr1 | CDS | 20245372 | 20245430 | 20245372 | ID=cds.MsaT001448.1;Parent=MsaT001448.1 |
| Chr1 | exon | 20245514 | 20245756 | 20245514 | ID=MsaT001448.1.exon3;Parent=MsaT001448.1 |
| Chr1 | CDS | 20245514 | 20245756 | 20245514 | ID=cds.MsaT001448.1;Parent=MsaT001448.1 |
| Gene Sequence |
| Protein sequence |
