AlfalfaGEDB | MsaG001455

M. sativa ssp. caerulea / MsaG001455

KEGG: -
GO: -
PFAM: PF02362.24

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG001455	XP_039688461.1	88.961	308	30	1	1	308	1	304	0.0	517

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG001455	sp\|Q8RYD1\|REM16_ARATH	24.549	277	173	5	28	279	36	301	1.64e-15	79.3

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG001455	tr\|A0A396JU70\|A0A396JU70_MEDTR	89.610	308	27	2	1	308	1	303	0.0	517

TFs/TRs:

Gene ID	Type	Classification
MsaG001455	TF	B3

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsaG001455	MsaG006279	0.814884	2.122619e-51	4.091195e-49
MsaG001455	MsaG021602	0.835524	2.912092e-56	9.908792e-54
MsaG001455	MsaG021767	0.827490	2.714156e-54	7.316548e-52
MsaG001455	MsaG025713	0.811506	1.160687e-50	2.056436e-48
MsaG001455	MsaG031211	0.841283	9.680941e-58	3.935009e-55

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG001455	MtrunA17_Chr1g0163221	89.610	308	27	2	1	308	1	303	0.0	517
MsaG001455	MtrunA17_Chr4g0067431	64.329	328	93	6	1	308	1	324	1.35e-141	402
MsaG001455	MtrunA17_Chr6g0467351	58.824	238	77	3	1	224	1	231	8.72e-93	275
MsaG001455	MtrunA17_Chr1g0163061	50.566	265	71	3	1	205	1	265	5.11e-67	211
MsaG001455	MtrunA17_Chr1g0163131	31.699	306	171	13	1	281	1	293	2.58e-32	122
MsaG001455	MtrunA17_Chr1g0163071	28.435	313	176	9	31	309	2	300	2.98e-29	113
MsaG001455	MtrunA17_Chr3g0131891	27.891	294	201	7	9	295	13	302	9.28e-29	112
MsaG001455	MtrunA17_Chr6g0467361	65.169	89	27	3	218	303	7	94	1.44e-28	105
MsaG001455	MtrunA17_Chr3g0103181	29.139	302	175	12	7	295	4	279	1.58e-27	108
MsaG001455	MtrunA17_Chr1g0163181	28.763	299	174	11	19	294	9	291	7.05e-27	109
MsaG001455	MtrunA17_Chr1g0163261	29.268	287	182	9	15	294	9	281	1.61e-26	106
MsaG001455	MtrunA17_Chr1g0163251	30.075	266	169	9	24	283	14	268	3.07e-26	107
MsaG001455	MtrunA17_Chr1g0163231	29.242	277	170	7	15	283	9	267	5.02e-26	104
MsaG001455	MtrunA17_Chr7g0233561	28.223	287	184	8	13	281	16	298	5.30e-26	105
MsaG001455	MtrunA17_Chr1g0208771	25.172	290	189	8	31	300	13	294	6.01e-26	107
MsaG001455	MtrunA17_Chr8g0390841	26.045	311	203	10	13	304	37	339	8.24e-26	107
MsaG001455	MtrunA17_Chr3g0103171	28.467	274	173	8	31	295	6	265	3.37e-24	99.0
MsaG001455	MtrunA17_Chr3g0103191	28.689	244	151	8	13	250	10	236	5.77e-24	98.2
MsaG001455	MtrunA17_Chr1g0163201	29.070	258	167	8	31	279	19	269	1.20e-23	100
MsaG001455	MtrunA17_Chr7g0233541	27.444	266	177	5	31	281	2	266	6.72e-23	96.3
MsaG001455	MtrunA17_Chr7g0233521	28.405	257	169	6	31	281	2	249	2.95e-22	94.0
MsaG001455	MtrunA17_Chr1g0163101	31.361	169	95	5	1	152	1	165	1.46e-21	90.1
MsaG001455	MtrunA17_Chr3g0103151	35.433	127	74	4	13	134	10	133	8.66e-20	84.7
MsaG001455	MtrunA17_Chr7g0233571	37.391	115	71	1	13	127	8	121	1.10e-19	85.9
MsaG001455	MtrunA17_Chr7g0233531	25.969	258	174	6	31	281	2	249	1.29e-19	87.0
MsaG001455	MtrunA17_Chr1g0171611	26.449	276	167	8	15	283	9	255	2.06e-19	86.7
MsaG001455	MtrunA17_Chr5g0419201	38.261	115	69	2	1	114	1	114	1.70e-18	82.0
MsaG001455	MtrunA17_Chr1g0154161	25.085	295	168	12	13	281	28	295	1.03e-16	79.3
MsaG001455	MtrunA17_Chr1g0163121	36.066	122	64	4	29	140	9	126	1.33e-16	75.5
MsaG001455	MtrunA17_Chr1g0154131	26.689	296	169	14	13	281	182	456	1.60e-16	79.7
MsaG001455	MtrunA17_Chr6g0476181	40.179	112	59	4	31	139	2	108	2.20e-16	75.5
MsaG001455	MtrunA17_Chr3g0077411	23.510	302	155	9	2	293	12	247	1.03e-15	77.4
MsaG001455	MtrunA17_Chr3g0077411	24.000	275	172	8	13	281	260	503	6.08e-13	69.3
MsaG001455	MtrunA17_Chr5g0429931	27.306	271	151	10	31	295	11	241	1.77e-15	74.7
MsaG001455	MtrunA17_Chr1g0163081	38.542	96	55	2	31	126	6	97	6.28e-15	70.9
MsaG001455	MtrunA17_Chr1g0162161	33.333	147	84	5	31	169	4	144	1.08e-14	70.5
MsaG001455	MtrunA17_Chr1g0154191	24.169	331	181	15	13	281	20	342	1.14e-14	73.9
MsaG001455	MtrunA17_Chr3g0077451	25.899	278	141	11	29	295	10	233	3.83e-14	70.9
MsaG001455	MtrunA17_Chr3g0077461	34.234	111	72	1	14	124	236	345	2.08e-13	70.5
MsaG001455	MtrunA17_Chr3g0077461	23.209	349	209	13	13	308	2	344	7.72e-11	62.8
MsaG001455	MtrunA17_Chr1g0163241	33.333	102	65	2	31	132	23	121	2.18e-13	67.0
MsaG001455	MtrunA17_Chr3g0103201	27.039	233	143	9	74	295	4	220	1.03e-12	66.2
MsaG001455	MtrunA17_Chr1g0163141	33.663	101	63	2	29	129	9	105	8.51e-12	63.2
MsaG001455	MtrunA17_Chr1g0154151	26.736	288	170	12	30	281	19	301	1.72e-11	64.3
MsaG001455	MtrunA17_Chr1g0163191	31.159	138	71	3	24	140	14	148	9.53e-11	59.7

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG001455	AT3G18990.2	25.872	344	192	13	15	302	6	342	1.62e-20	91.3
MsaG001455	AT3G18990.1	26.006	323	177	12	15	281	6	322	2.12e-20	90.5
MsaG001455	AT4G33280.1	24.549	277	173	5	28	279	36	301	1.67e-16	79.3
MsaG001455	AT3G18960.1	36.634	101	63	1	15	115	30	129	2.31e-15	73.9
MsaG001455	AT3G18960.2	36.634	101	63	1	15	115	30	129	2.85e-15	73.6
MsaG001455	AT4G01580.1	34.653	101	65	1	15	115	30	129	4.80e-14	70.1
MsaG001455	AT1G49475.1	35.135	111	70	2	15	125	34	142	2.78e-13	67.8
MsaG001455	AT3G18960.3	37.647	85	52	1	31	115	9	92	2.24e-11	62.0

Find 35 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ATTTATCTTTGTCAACAAAA+AGG	0.196489	1:+20290498	None:intergenic
GAAGAGAATAATTTGAAATT+TGG	0.198679	1:-20290413	MsaT001455.1:CDS
CTCTATCAATGTATGACTTT+TGG	0.220204	1:+20290889	None:intergenic
CTTCATCATCACGGGTTAAA+AGG	0.256061	1:-20291201	MsaT001455.1:CDS
TTTGGAAACCTTAGAGTAAC+TGG	0.256715	1:+20291472	None:intergenic
AAGTAGTTGTGATGATATTA+AGG	0.299219	1:-20290985	MsaT001455.1:CDS
GTCTTTAATGTGATTGTATT+CGG	0.330391	1:-20291313	MsaT001455.1:CDS
TTCTTACAAACTTCCATAGA+TGG	0.339692	1:+20291163	None:intergenic
ATATAATTACCTTCAAAATA+AGG	0.343429	1:+20290302	None:intergenic
TAACAAACCCAGTTACTCTA+AGG	0.363012	1:-20291480	MsaT001455.1:CDS
ATTTCAAATTATTCTCTTCA+CGG	0.368199	1:+20290417	None:intergenic
GAGAAAATAACAACTTGAAA+TGG	0.395512	1:+20290347	None:intergenic
TTTCTCTTTGGAAAAGGACT+TGG	0.399841	1:-20290330	MsaT001455.1:CDS
TGTTTCTTGACAAATTCATT+TGG	0.414955	1:+20290554	None:intergenic
CTTCCCTAGTTTCTCTTGAC+GGG	0.422598	1:+20291266	None:intergenic
TCTTCCCTAGTTTCTCTTGA+CGG	0.433773	1:+20291265	None:intergenic
TGAATTTGTCAAGAAACATA+TGG	0.468376	1:-20290549	MsaT001455.1:CDS
TCTAAGGTTTCCAAACATGA+TGG	0.492653	1:-20291464	MsaT001455.1:CDS
TGAGAACCCTTCATCATCAC+GGG	0.503349	1:-20291209	MsaT001455.1:CDS
CAAGAAACATATGGACATGA+TGG	0.508763	1:-20290540	MsaT001455.1:CDS
AATACCCGTCAAGAGAAACT+AGG	0.533719	1:-20291270	MsaT001455.1:CDS
ATACATTGATAGAGATCGCT+TGG	0.539899	1:-20290880	MsaT001455.1:intron
TTTGTGAAAAGGTATTGGAA+AGG	0.553882	1:-20291505	MsaT001455.1:CDS
AATCTGCACTAACTTGTGGT+TGG	0.573287	1:-20290448	MsaT001455.1:CDS
TTGAGAACCCTTCATCATCA+CGG	0.575621	1:-20291210	MsaT001455.1:CDS
TTTGTGCTAAACAAAGGCAA+AGG	0.576418	1:-20290377	MsaT001455.1:CDS
ATACCCGTCAAGAGAAACTA+GGG	0.582374	1:-20291269	MsaT001455.1:CDS
AAACAATCTGCACTAACTTG+TGG	0.589005	1:-20290452	MsaT001455.1:CDS
GAACATGGTCATCAGAGTAA+TGG	0.594006	1:-20291999	MsaT001455.1:CDS
AAACATATGGACATGATGGA+AGG	0.599445	1:-20290536	MsaT001455.1:CDS
ACCATGTTCTTGAGAAGACA+TGG	0.602247	1:+20292013	None:intergenic
GTGATTGTATTCGGTAGATG+CGG	0.604317	1:-20291304	MsaT001455.1:CDS
TTGGAAACCTTAGAGTAACT+GGG	0.606122	1:+20291473	None:intergenic
ACCATGTCTTCTCAAGAACA+TGG	0.657917	1:-20292014	MsaT001455.1:CDS
AAGCGTGAGCATGTGAACAA+TGG	0.705898	1:-20291106	MsaT001455.1:intron

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content

Chromosome	Type	Strat	End	Strand	Name
Chr1	gene	20290298	20292033	20290298	ID=MsaG001455
Chr1	mRNA	20290298	20292033	20290298	ID=MsaT001455.1;Parent=MsaG001455
Chr1	exon	20290298	20290582	20290298	ID=MsaT001455.1.exon4;Parent=MsaT001455.1
Chr1	CDS	20290298	20290582	20290298	ID=cds.MsaT001455.1;Parent=MsaT001455.1
Chr1	exon	20290881	20291008	20290881	ID=MsaT001455.1.exon3;Parent=MsaT001455.1
Chr1	CDS	20290881	20291008	20290881	ID=cds.MsaT001455.1;Parent=MsaT001455.1
Chr1	exon	20291107	20291542	20291107	ID=MsaT001455.1.exon2;Parent=MsaT001455.1
Chr1	CDS	20291107	20291542	20291107	ID=cds.MsaT001455.1;Parent=MsaT001455.1
Chr1	exon	20291947	20292033	20291947	ID=MsaT001455.1.exon1;Parent=MsaT001455.1
Chr1	CDS	20291947	20292033	20291947	ID=cds.MsaT001455.1;Parent=MsaT001455.1

Gene Sequence

>MsaT001455.1

ATGTCTTCTCAAGAACATGGTCATCAGAGTAATGGTGTCCACTTTTTTAAGGTTATTCGTCAAAGTACCCTTCAAGAAGAAAAACTGAGTGTGCCCATAACTTTTGTGAAAAGGTATTGGAAAGGAATAACAAACCCAGTTACTCTAAGGTTTCCAAACATGATGGAAAAGAAAGTTTTTTGGGAGAAAACTAGTGAAAATGAAGTTATATTTTGCAATGGTTGGAAAGAACTTGCAAATTATTTGTCTTTGAATGAATCACAATTTTTGGTGTTTCAATATCAAGAAAATTCTGTCTTTAATGTGATTGTATTCGGTAGATGCGGATTAGAAATAAAATACCCGTCAAGAGAAACTAGGGAAGAATCTGAAGAGAGTGATAGTTCTTTGAAAATTATTGAGAACCCTTCATCATCACGGGTTAAAAGGCTAAAATTTTCAGCACCATCTATGGAAGTTTGTAAGAAGATGAAAATCAACTCAAAAGAACAAAAAGATTCTAAGCGTGAGCATGTGAACAATGGAAGTAGTTGTGATGATATTAAGGAGAGAAGTAGAATTTTGTTCCAGAAAGTGAAGAAAAAATTCGACTCTGATAAGGACTTTTTTGCATGTATGATCCAAAAGTCATACATTGATAGAGATCGCTTGGTCATACCAAATGAATTTGTCAAGAAACATATGGACATGATGGAAGGAAGAGATGATGCTACCCTTTTTGTTGACAAAGATAAATGGAATTTGAAGCTCACTTTAAATAAACAATCTGCACTAACTTGTGGTTGGAGAGAATTCCGTGAAGAGAATAATTTGAAATTTGGTGATGTTTGTGTTTTTGTGCTAAACAAAGGCAAAGGAACAGTTCCATTTCAAGTTGTTATTTTCTCTTTGGAAAAGGACTTGGAAACTCCTTATTTTGAAGGTAATTATATTTGA

Protein sequence

>MsaT001455.1

MSSQEHGHQSNGVHFFKVIRQSTLQEEKLSVPITFVKRYWKGITNPVTLRFPNMMEKKVFWEKTSENEVIFCNGWKELANYLSLNESQFLVFQYQENSVFNVIVFGRCGLEIKYPSRETREESEESDSSLKIIENPSSSRVKRLKFSAPSMEVCKKMKINSKEQKDSKREHVNNGSSCDDIKERSRILFQKVKKKFDSDKDFFACMIQKSYIDRDRLVIPNEFVKKHMDMMEGRDDATLFVDKDKWNLKLTLNKQSALTCGWREFREENNLKFGDVCVFVLNKGKGTVPFQVVIFSLEKDLETPYFEGNYI*