AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG001460


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG001460 MtrunA17_Chr1g0163261 87.919 298 36 0 1 298 1 298 0.0 528
MsaG001460 MtrunA17_Chr1g0163181 44.776 469 199 7 1 441 1 437 5.34e-128 377
MsaG001460 MtrunA17_Chr1g0163201 47.285 442 152 9 24 442 18 401 6.48e-125 368
MsaG001460 MtrunA17_Chr1g0163231 56.376 298 100 9 1 285 1 281 1.82e-101 304
MsaG001460 MtrunA17_Chr1g0171611 54.671 289 107 8 1 285 1 269 6.51e-90 275
MsaG001460 MtrunA17_Chr1g0163251 48.204 334 153 8 1 325 1 323 6.34e-89 276
MsaG001460 MtrunA17_Chr1g0163151 45.421 273 97 5 162 434 2 222 1.11e-70 223
MsaG001460 MtrunA17_Chr1g0163111 46.743 261 105 5 176 435 3 230 2.70e-70 222
MsaG001460 MtrunA17_Chr6g0476171 40.357 280 124 6 157 433 6 245 8.52e-54 180
MsaG001460 MtrunA17_Chr1g0162151 35.926 270 107 5 163 432 3 206 3.87e-44 154
MsaG001460 MtrunA17_Chr8g0390841 27.495 491 278 19 8 432 38 516 3.42e-41 153
MsaG001460 MtrunA17_Chr1g0163241 53.191 141 57 3 24 155 22 162 2.00e-39 139
MsaG001460 MtrunA17_Chr3g0131891 31.987 297 182 9 6 287 16 307 1.01e-34 132
MsaG001460 MtrunA17_Chr1g0163091 50.365 137 62 2 163 299 3 133 2.26e-34 126
MsaG001460 MtrunA17_Chr7g0233561 31.290 310 169 10 6 283 15 312 5.50e-34 129
MsaG001460 MtrunA17_Chr7g0233541 33.571 280 164 8 25 283 2 280 1.67e-32 125
MsaG001460 MtrunA17_Chr1g0163191 58.696 92 38 0 18 109 14 105 2.09e-32 120
MsaG001460 MtrunA17_Chr1g0208771 25.926 459 276 15 24 432 12 456 3.40e-31 124
MsaG001460 MtrunA17_Chr7g0233531 34.559 272 155 10 25 283 2 263 7.11e-31 120
MsaG001460 MtrunA17_Chr7g0233521 33.564 289 135 11 25 283 2 263 1.10e-30 119
MsaG001460 MtrunA17_Chr4g0067431 28.912 294 173 9 9 273 15 301 5.51e-29 115
MsaG001460 MtrunA17_Chr1g0163101 40.764 157 80 4 9 155 17 170 1.06e-26 105
MsaG001460 MtrunA17_Chr1g0163131 27.027 296 195 7 5 282 14 306 2.03e-26 108
MsaG001460 MtrunA17_Chr1g0163131 43.262 141 65 5 299 435 179 308 1.18e-19 89.4
MsaG001460 MtrunA17_Chr1g0162161 40.411 146 66 4 25 155 4 143 2.10e-25 101
MsaG001460 MtrunA17_Chr1g0163221 29.562 274 176 8 9 271 11 278 2.94e-25 106
MsaG001460 MtrunA17_Chr7g0233571 34.434 212 119 6 4 197 5 214 5.18e-25 102
MsaG001460 MtrunA17_Chr1g0163071 26.510 298 178 5 25 288 2 292 1.41e-23 100
MsaG001460 MtrunA17_Chr1g0163081 38.849 139 73 3 25 155 6 140 2.72e-21 89.7
MsaG001460 MtrunA17_Chr1g0163141 33.333 162 78 4 23 155 9 169 3.98e-21 90.5
MsaG001460 MtrunA17_Chr1g0163121 50.575 87 42 1 23 109 9 94 5.04e-21 89.0
MsaG001460 MtrunA17_Chr5g0419201 40.741 108 62 1 3 108 10 117 7.01e-21 90.1
MsaG001460 MtrunA17_Chr6g0476181 39.024 123 74 1 25 147 2 123 2.55e-20 87.8
MsaG001460 MtrunA17_Chr3g0103181 25.714 280 194 6 6 281 9 278 3.28e-20 90.5
MsaG001460 MtrunA17_Chr3g0103151 39.370 127 68 3 6 130 9 128 4.54e-20 87.0
MsaG001460 MtrunA17_Chr3g0103191 39.167 120 68 3 6 122 9 126 2.99e-18 84.0
MsaG001460 MtrunA17_Chr6g0467351 37.121 132 74 4 9 137 15 140 5.26e-18 82.8
MsaG001460 MtrunA17_Chr3g0103171 22.989 261 197 3 24 281 5 264 2.84e-17 81.6
MsaG001460 MtrunA17_Chr1g0163161 35.606 132 68 4 37 155 1 128 2.70e-14 69.7
MsaG001460 MtrunA17_Chr1g0163061 39.000 100 58 2 9 105 15 114 1.48e-13 70.9
MsaG001460 MtrunA17_Chr5g0419211 35.789 95 58 2 205 298 11 103 6.11e-12 62.4
MsaG001460 MtrunA17_Chr3g0077411 23.427 286 174 9 9 281 262 515 2.06e-11 65.9
MsaG001460 MtrunA17_Chr4g0033961 33.945 109 70 1 9 115 6 114 2.32e-11 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG001460 AT3G18990.1 25.731 342 189 11 6 286 2 339 3.42e-20 91.7
MsaG001460 AT3G18990.2 25.439 342 190 11 6 286 2 339 1.36e-19 90.5
MsaG001460 AT4G01580.1 31.469 143 92 3 7 147 28 166 4.34e-17 79.7
MsaG001460 AT3G18960.2 28.662 157 94 4 7 147 28 182 8.72e-12 64.3
MsaG001460 AT3G18960.1 28.662 157 94 4 7 147 28 182 2.33e-11 63.5

Find 62 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATTATCATTATGGATTTAT+TGG 0.145526 1:-20348815 MsaT001460.1:CDS
GTGCTAAACAAGATGTATTT+TGG 0.183994 1:-20350416 MsaT001460.1:CDS
AAAATTATATATTATCATTA+TGG 0.218455 1:-20348824 MsaT001460.1:CDS
TTATGCCAAAGGATGTTATT+TGG 0.223238 1:-20349878 MsaT001460.1:intron
TTCAGCTTCAACCTTTATAT+TGG 0.225744 1:+20350242 None:intergenic
TGTTTCAAAGTTGAAATATT+TGG 0.227189 1:-20350291 MsaT001460.1:CDS
GTGTTTAATGTTTCAATTGT+AGG 0.249847 1:-20348399 MsaT001460.1:CDS
TGACATTTCAACTTCAAAAC+TGG 0.265312 1:+20349906 None:intergenic
TGTAGCTCCACAAAATATTT+AGG 0.280715 1:+20350483 None:intergenic
GAAAAGGTGGCAAGAGAAAA+AGG 0.281037 1:-20350155 MsaT001460.1:CDS
ACAAGAGATAAAACAGCTAA+TGG 0.319359 1:+20348369 None:intergenic
ACATCTTGTTTAGCACCATT+TGG 0.328942 1:+20350423 None:intergenic
AAGAGGATGTCATGAAAATT+TGG 0.332779 1:-20348583 MsaT001460.1:CDS
GCAAAGAGCAAAGACAATAA+AGG 0.337712 1:-20349109 MsaT001460.1:CDS
AATTATTATCGTAGTATTCA+TGG 0.337928 1:-20348519 MsaT001460.1:CDS
TAATTAATCGTACCGGGTAT+TGG 0.348306 1:+20348782 None:intergenic
TGGAGCGTGACGGAGATATT+TGG 0.354418 1:-20350392 MsaT001460.1:CDS
ATAAAGGGACTTCAAGTTCT+AGG 0.354574 1:-20349093 MsaT001460.1:CDS
CAAAATCAAGAACTGCAAAA+TGG 0.354682 1:-20350629 MsaT001460.1:CDS
AATAAACTATTCCAATATAA+AGG 0.363838 1:-20350253 MsaT001460.1:CDS
AGCAAGAAATTATCATCCTT+TGG 0.369605 1:-20349935 MsaT001460.1:CDS
AATTAATCGTACCGGGTATT+GGG 0.378367 1:+20348783 None:intergenic
CATCTTGTTTAGCACCATTT+GGG 0.395094 1:+20350424 None:intergenic
ACATACCAAATAACATCCTT+TGG 0.421503 1:+20349873 None:intergenic
CAAAGAGCAAAGACAATAAA+GGG 0.423420 1:-20349108 MsaT001460.1:CDS
ATCGTAGTATTCATGGTTGT+AGG 0.430779 1:-20348512 MsaT001460.1:CDS
AAAGAATGCAATGTGGAGAT+TGG 0.431673 1:-20348462 MsaT001460.1:CDS
CATGGTTGTAGGTTCTCTGC+AGG 0.437756 1:-20348501 MsaT001460.1:CDS
AAAATATATAATTTGAAACT+TGG 0.446327 1:-20349357 MsaT001460.1:CDS
CCAATGAAGTTTATGAAAAG+AGG 0.467022 1:-20348600 MsaT001460.1:CDS
CAAGAGATAAAACAGCTAAT+GGG 0.468737 1:+20348370 None:intergenic
AACTTTGCAGGTTGTAATAG+AGG 0.470896 1:-20350012 MsaT001460.1:intron
TTTGGGAGAGTAAAGACTAC+TGG 0.484305 1:+20350441 None:intergenic
GACAATGTTACTAATACTAA+TGG 0.484869 1:-20350198 MsaT001460.1:CDS
AGTACATCTCAAAGGAGAAA+AGG 0.489671 1:-20350171 MsaT001460.1:CDS
AATATTTGGTCGCAATACAT+TGG 0.491389 1:-20350277 MsaT001460.1:CDS
CTCCTCTCTTGCTCGAGTAA+TGG 0.510615 1:+20349281 None:intergenic
TTTAAGAAGTTGAAGTTGCA+AGG 0.514255 1:-20349252 MsaT001460.1:intron
GACATTTCAACTTCAAAACT+GGG 0.526404 1:+20349907 None:intergenic
GTTGTAGGTTCTCTGCAGGT+TGG 0.528959 1:-20348497 MsaT001460.1:CDS
ATGATCTTGAAGAACTTTCG+AGG 0.530699 1:+20350653 None:intergenic
ATGGTAATAGTGAATAAAAG+TGG 0.534500 1:+20349300 None:intergenic
GTAGTCTTTACTCTCCCAAA+TGG 0.552023 1:-20350438 MsaT001460.1:CDS
CATCTACTGTGACCACTGGT+TGG 0.552617 1:-20349392 MsaT001460.1:CDS
TAGAGGAGGCAAGATTAAGA+AGG 0.558763 1:-20349995 MsaT001460.1:CDS
AAATCATCTACTGTGACCAC+TGG 0.561649 1:-20349396 MsaT001460.1:CDS
AGGATGTCATGAAAATTTGG+TGG 0.562321 1:-20348580 MsaT001460.1:CDS
AACTTCAACTTCTTAAAGCT+AGG 0.570828 1:+20349258 None:intergenic
TACCATTACTCGAGCAAGAG+AGG 0.576066 1:-20349283 MsaT001460.1:CDS
TAATAGTGAATAAAAGTGGT+TGG 0.584021 1:+20349304 None:intergenic
ATGTCACGATCTTATGCCAA+AGG 0.590157 1:-20349889 MsaT001460.1:CDS
TTCATCAACTCTAAATCTTG+CGG 0.597151 1:+20349461 None:intergenic
GGTTATGAATTCCCAATACC+CGG 0.612665 1:-20348794 MsaT001460.1:intron
GCTAAATGACTTCCAACCAG+TGG 0.615117 1:+20349380 None:intergenic
TGATCTTGAAGAACTTTCGA+GGG 0.617342 1:+20350654 None:intergenic
ACATCTCAAAGGAGAAAAGG+TGG 0.624210 1:-20350168 MsaT001460.1:CDS
ATTTAGAGTTGATGAAGACA+TGG 0.625733 1:-20349454 MsaT001460.1:CDS
TTTGCAGGTTGTAATAGAGG+AGG 0.626297 1:-20350009 MsaT001460.1:intron
ATGGTGCTAGTACATCTCAA+AGG 0.644614 1:-20350179 MsaT001460.1:CDS
TATTGAAACAGAGGATACCA+AGG 0.667785 1:-20349521 MsaT001460.1:intron
ATTGCGAAATCTCTTGTACG+TGG 0.685092 1:-20350348 MsaT001460.1:CDS
ATTCTTAAAAGAATGCAATG+TGG 0.699756 1:-20348469 MsaT001460.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr1 gene 20348365 20350690 20348365 ID=MsaG001460
Chr1 mRNA 20348365 20350690 20348365 ID=MsaT001460.1;Parent=MsaG001460
Chr1 exon 20348365 20348628 20348365 ID=MsaT001460.1.exon7;Parent=MsaT001460.1
Chr1 CDS 20348365 20348628 20348365 ID=cds.MsaT001460.1;Parent=MsaT001460.1
Chr1 exon 20348795 20348886 20348795 ID=MsaT001460.1.exon6;Parent=MsaT001460.1
Chr1 CDS 20348795 20348886 20348795 ID=cds.MsaT001460.1;Parent=MsaT001460.1
Chr1 exon 20349065 20349154 20349065 ID=MsaT001460.1.exon5;Parent=MsaT001460.1
Chr1 CDS 20349065 20349154 20349065 ID=cds.MsaT001460.1;Parent=MsaT001460.1
Chr1 exon 20349253 20349532 20349253 ID=MsaT001460.1.exon4;Parent=MsaT001460.1
Chr1 CDS 20349253 20349532 20349253 ID=cds.MsaT001460.1;Parent=MsaT001460.1
Chr1 exon 20349879 20350024 20349879 ID=MsaT001460.1.exon3;Parent=MsaT001460.1
Chr1 CDS 20349879 20350024 20349879 ID=cds.MsaT001460.1;Parent=MsaT001460.1
Chr1 exon 20350118 20350511 20350118 ID=MsaT001460.1.exon2;Parent=MsaT001460.1
Chr1 CDS 20350118 20350511 20350118 ID=cds.MsaT001460.1;Parent=MsaT001460.1
Chr1 exon 20350622 20350690 20350622 ID=MsaT001460.1.exon1;Parent=MsaT001460.1
Chr1 CDS 20350622 20350690 20350622 ID=cds.MsaT001460.1;Parent=MsaT001460.1
Gene Sequence

>MsaT001460.1

ATGTCATTTTATCACCCTCGAAAGTTCTTCAAGATCATGCAAAATCAAGAACTGCAAAATGGAGAGCTTAGGGTTCCTAAATATTTTGTGGAGCTACATTGGAAAAATGTATCAAATCCAGTAGTCTTTACTCTCCCAAATGGTGCTAAACAAGATGTATTTTGGGTGGAGCGTGACGGAGATATTTGGTTTCAAAAAAATTGGGAAAAAATTGCGAAATCTCTTGTACGTGGACATGTTCTTGTTTTTAAATATAAAGGAGGATCATGTTTCAAAGTTGAAATATTTGGTCGCAATACATTGGAAATAAACTATTCCAATATAAAGGTTGAAGCTGAAGAAGTTGTTACAGTGAGTGATGACAATGTTACTAATACTAATGGTGCTAGTACATCTCAAAGGAGAAAAGGTGGCAAGAGAAAAAGGAATATGGATCTTGATGCTAATCAGAAAAAAATCAAAGGTTGTAATAGAGGAGGCAAGATTAAGAAGGCTAAAAAATGCTTAAGAACTGAAGCTGTTAAAGAGAGAGCAAGAAATTATCATCCTTTGGAAAATCCCAGTTTTGAAGTTGAAATGTCACGATCTTATGCCAAAGGATGTTATTTGAGGATACCAAGGTATTTTTCGAGAAAATACTTGAAAAAATTGGAAGGGACCGCAAGATTTAGAGTTGATGAAGACATGGCTATAGAAGTGAAGTTGAATTTTGATGATGCTACTAAATCATCTACTGTGACCACTGGTTGGAAGTCATTTAGCAAAATATATAATTTGAAACTTGGTGATGTGTGCAAATTTGTGATGACTCAATGCCAACCACTTTTATTCACTATTACCATTACTCGAGCAAGAGAGGAGCCTAGCTTTAAGAAGTTGAAGTTGCAAGGGATCTCTTCTAGTAATAACAATGCAAAGAGCAAAGACAATAAAGGGACTTCAAGTTCTAGGAGCTGCTCAAAAACACATCTTCTCGGGAACTTGTTTAAAAATCTCAAAATAATATATGGATTTATTGTAAAATTATATATTATCATTATGGATTTATTGGTTATGAATTCCCAATACCCGGATGTACCAATGAAGTTTATGAAAAGAGGATGTCATGAAAATTTGGTGGAGTTAAAAATGGGGGGACGATCTTGGTTTGTAAAAGTGAATTATTATCGTAGTATTCATGGTTGTAGGTTCTCTGCAGGTTGGAAACAATTCTTAAAAGAATGCAATGTGGAGATTGGAGATACTTGTCTCTTGAAGATGATTGATGAGAAAAAATTTGTGTTTAATGTTTCAATTGTAGGAAAGAACCCATTAGCTGTTTTATCTCTTGTTTAA

Protein sequence

>MsaT001460.1

MSFYHPRKFFKIMQNQELQNGELRVPKYFVELHWKNVSNPVVFTLPNGAKQDVFWVERDGDIWFQKNWEKIAKSLVRGHVLVFKYKGGSCFKVEIFGRNTLEINYSNIKVEAEEVVTVSDDNVTNTNGASTSQRRKGGKRKRNMDLDANQKKIKGCNRGGKIKKAKKCLRTEAVKERARNYHPLENPSFEVEMSRSYAKGCYLRIPRYFSRKYLKKLEGTARFRVDEDMAIEVKLNFDDATKSSTVTTGWKSFSKIYNLKLGDVCKFVMTQCQPLLFTITITRAREEPSFKKLKLQGISSSNNNAKSKDNKGTSSSRSCSKTHLLGNLFKNLKIIYGFIVKLYIIIMDLLVMNSQYPDVPMKFMKRGCHENLVELKMGGRSWFVKVNYYRSIHGCRFSAGWKQFLKECNVEIGDTCLLKMIDEKKFVFNVSIVGKNPLAVLSLV*