AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG001567

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG001567 MtrunA17_Chr1g0164881 93.617 235 11 1 1 231 1 235 5.74e-130 366
MsaG001567 MtrunA17_Chr1g0211941 63.115 244 54 5 16 229 18 255 5.19e-84 250
MsaG001567 MtrunA17_Chr2g0277361 75.000 84 18 1 20 100 115 198 1.38e-36 133
MsaG001567 MtrunA17_Chr5g0416581 78.205 78 12 1 28 100 58 135 1.60e-36 132
MsaG001567 MtrunA17_Chr7g0224581 54.400 125 53 1 1 121 15 139 2.94e-36 129
MsaG001567 MtrunA17_Chr4g0060601 70.930 86 21 1 19 100 80 165 7.24e-36 131
MsaG001567 MtrunA17_Chr1g0180911 78.082 73 13 1 25 94 39 111 5.00e-35 124
MsaG001567 MtrunA17_Chr1g0204001 65.934 91 30 1 5 94 36 126 9.13e-33 121
MsaG001567 MtrunA17_Chr8g0350941 72.603 73 20 0 28 100 76 148 1.86e-31 117
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG001567 AT5G23280.1 56.800 250 62 6 10 231 19 250 9.09e-75 227
MsaG001567 AT5G08330.1 57.384 237 64 8 16 229 13 235 1.84e-63 197
MsaG001567 AT1G72010.1 81.944 72 13 0 29 100 60 131 5.06e-37 133
MsaG001567 AT1G69690.1 68.889 90 23 1 16 100 31 120 5.60e-37 132
MsaG001567 AT3G47620.1 67.708 96 25 2 11 100 90 185 1.51e-36 134
MsaG001567 AT1G58100.1 84.058 69 11 0 32 100 60 128 7.22e-36 131
MsaG001567 AT1G35560.1 83.333 72 12 0 29 100 52 123 9.09e-35 127
MsaG001567 AT3G27010.2 63.000 100 28 2 3 93 40 139 1.34e-33 123
MsaG001567 AT3G27010.1 63.000 100 28 2 3 93 40 139 1.34e-33 123
MsaG001567 AT5G51910.1 67.059 85 28 0 28 112 53 137 4.68e-32 119
MsaG001567 AT5G51910.2 67.059 85 28 0 28 112 53 137 4.68e-32 119
MsaG001567 AT2G45680.1 76.471 68 16 0 27 94 70 137 1.29e-31 119
MsaG001567 AT5G41030.1 67.164 67 22 0 28 94 64 130 4.31e-28 107
MsaG001567 AT2G37000.1 75.000 60 15 0 32 91 43 102 4.55e-27 103
MsaG001567 AT3G45150.1 52.055 73 35 0 33 105 18 90 6.83e-20 83.6
MsaG001567 AT1G58100.2 55.072 69 7 1 32 100 60 104 5.50e-15 73.6

Find 61 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACACGTGCGGCGCAGATTA+TGG 0.191163 1:+22564878 None:intergenic
CTACTGCTCCGCCGGGGTTT+TGG 0.252668 1:-22564635 MsaT001567.1:CDS
CAACCTTCTCCGTTTATTCT+TGG 0.258658 1:-22564705 MsaT001567.1:CDS
AATTTGTTGGCTTCATTGTC+TGG 0.298075 1:-22564393 MsaT001567.1:CDS
GAAGCTTCGGCGGCGAGGTT+AGG 0.304875 1:-22564438 MsaT001567.1:CDS
TCAACAGCAACAAGGCTCTA+TGG 0.307887 1:-22564463 MsaT001567.1:CDS
GTTGATGCTGCTGGTGGTGC+TGG 0.308604 1:+22564481 None:intergenic
GACCAAGCTCACGTGTTAAC+TGG 0.329708 1:+22564853 None:intergenic
GCTGCTGGTGGTGCTGGTGC+TGG 0.334270 1:+22564487 None:intergenic
TCCTGGTCATCTGAATTTGT+TGG 0.361921 1:-22564406 MsaT001567.1:CDS
ACACGTGCGGCGCAGATTAT+GGG 0.390746 1:+22564879 None:intergenic
CTTGTTGCTGTTGATGCTGC+TGG 0.395955 1:+22564472 None:intergenic
TGCTGCACCTACTGCTCCGC+CGG 0.410157 1:-22564643 MsaT001567.1:CDS
GAAAGAAGCAGGAGTAGTTC+CGG 0.413992 1:+22564758 None:intergenic
TGATGATGGTGATGCTGTTT+CGG 0.418538 1:-22564667 MsaT001567.1:CDS
TCTGGTGGGAATGGAAATTC+TGG 0.419287 1:-22564375 MsaT001567.1:CDS
AAGCTTCGGCGGCGAGGTTA+GGG 0.419803 1:-22564437 MsaT001567.1:CDS
AACGGAGAAGGTTGAAGAAA+AGG 0.424552 1:+22564714 None:intergenic
TGCTGCTGCTCAACCGCCGC+CGG 0.429682 1:-22564580 MsaT001567.1:CDS
GCTGCTCAACCGCCGCCGGA+AGG 0.449544 1:-22564576 MsaT001567.1:CDS
CTCCAAAGCTGAGCAAAATC+CGG 0.465288 1:+22564609 None:intergenic
ATCATCGCCGCCACCGGTAC+CGG 0.476622 1:-22564777 MsaT001567.1:CDS
CAACAGCAACAAGGCTCTAT+GGG 0.478969 1:-22564462 MsaT001567.1:CDS
AGTAGTTCCGGTACCGGTGG+CGG 0.480445 1:+22564770 None:intergenic
GCTGCACCTACTGCTCCGCC+GGG 0.481411 1:-22564642 MsaT001567.1:CDS
GCTTCATTGTCTGGTGGGAA+TGG 0.484637 1:-22564384 MsaT001567.1:CDS
AGGTTAGGGAATTATCTTCC+TGG 0.487752 1:-22564423 MsaT001567.1:CDS
AGAAGTGGGTTCTACTAGAT+CGG 0.502592 1:+22564983 None:intergenic
AAACGCTTACCAAGAATAAA+CGG 0.502790 1:+22564696 None:intergenic
AGGCTCTATGGGTGAAGCTT+CGG 0.504366 1:-22564451 MsaT001567.1:CDS
GAAAGCGAAGAGAAAGAAGC+AGG 0.509433 1:+22564747 None:intergenic
TGATCGACGGTTCAGCTTGA+CGG 0.511819 1:+22564796 None:intergenic
AGCAGGAGTAGTTCCGGTAC+CGG 0.517055 1:+22564764 None:intergenic
TTCCAGTTAACACGTGAGCT+TGG 0.518020 1:-22564855 MsaT001567.1:CDS
TTCCGTCTACTTTACTGTGA+CGG 0.524053 1:+22564922 None:intergenic
AAATCCGGCCGCCAAAACCC+CGG 0.528380 1:+22564624 None:intergenic
TGTTGGCTTCATTGTCTGGT+GGG 0.531348 1:-22564389 MsaT001567.1:CDS
CCGTCGATCATCGCCGCCAC+CGG 0.537613 1:-22564783 MsaT001567.1:CDS
CACAGTAAAGTAGACGGAAG+AGG 0.537838 1:-22564918 MsaT001567.1:CDS
CAAAACCCCGGCGGAGCAGT+AGG 0.540114 1:+22564636 None:intergenic
TTGTTGGCTTCATTGTCTGG+TGG 0.543932 1:-22564390 MsaT001567.1:CDS
GGGAATGGAAATTCTGGTCG+TGG 0.548380 1:-22564369 MsaT001567.1:CDS
TTCTACTAGATCGGTTGTTG+TGG 0.553500 1:+22564992 None:intergenic
GCCAACAAATTCAGATGACC+AGG 0.560326 1:+22564405 None:intergenic
CCGGTGGCGGCGATGATCGA+CGG 0.572268 1:+22564783 None:intergenic
TGATAGTTGTTGTTTCCTTG+TGG 0.582444 1:+22565015 None:intergenic
GTTGCTGTTGATGCTGCTGG+TGG 0.588478 1:+22564475 None:intergenic
CTCTATGGGTGAAGCTTCGG+CGG 0.604764 1:-22564448 MsaT001567.1:CDS
AGGAGTAGTTCCGGTACCGG+TGG 0.618720 1:+22564767 None:intergenic
CCATTCGATAGTTTGACCGT+CGG 0.631339 1:+22564824 None:intergenic
CAGCAGCATCAACAGCAACA+AGG 0.632046 1:-22564471 MsaT001567.1:CDS
ACCTCAAATGTCTAACCACA+AGG 0.638869 1:-22565030 None:intergenic
GACCGTCACAGTAAAGTAGA+CGG 0.641647 1:-22564924 MsaT001567.1:CDS
AAGCGTTTACGTGATGATGA+TGG 0.641838 1:-22564681 MsaT001567.1:CDS
CCGACGGTCAAACTATCGAA+TGG 0.646959 1:-22564824 MsaT001567.1:CDS
TTACCAAGAATAAACGGAGA+AGG 0.653545 1:+22564702 None:intergenic
TGGGTGAAGCTTCGGCGGCG+AGG 0.659727 1:-22564443 MsaT001567.1:CDS
GAGCTTGGTCATAAATCCGA+CGG 0.680249 1:-22564840 MsaT001567.1:CDS
CTGCACCTACTGCTCCGCCG+GGG 0.682389 1:-22564641 MsaT001567.1:CDS
TCCGGCCGCCAAAACCCCGG+CGG 0.699527 1:+22564627 None:intergenic
TGTTAACTGGAAAACACGTG+CGG 0.728936 1:+22564866 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr1 gene 22564350 22565045 22564350 ID=MsaG001567
Chr1 mRNA 22564350 22565045 22564350 ID=MsaT001567.1;Parent=MsaG001567
Chr1 exon 22564350 22565045 22564350 ID=MsaT001567.1.exon1;Parent=MsaT001567.1
Chr1 CDS 22564350 22565045 22564350 ID=cds.MsaT001567.1;Parent=MsaT001567.1
Gene Sequence

>MsaT001567.1

ATGTCTAACCACAAGGAAACAACAACTATCACCACAACAACCGATCTAGTAGAACCCACTTCTCAAAACACTCTAACAGTAAAAAAACCGCCGTCAAAAGACCGTCACAGTAAAGTAGACGGAAGAGGAAGACGCATAAGAATGCCCATAATCTGCGCCGCACGTGTTTTCCAGTTAACACGTGAGCTTGGTCATAAATCCGACGGTCAAACTATCGAATGGCTTCTCCGTCAAGCTGAACCGTCGATCATCGCCGCCACCGGTACCGGAACTACTCCTGCTTCTTTCTCTTCGCTTTCTGAGCATAAACCTTTTCTTCAACCTTCTCCGTTTATTCTTGGTAAGCGTTTACGTGATGATGATGGTGATGCTGTTTCGGTTGCTGCACCTACTGCTCCGCCGGGGTTTTGGCGGCCGGATTTTGCTCAGCTTTGGAGCTTTGCTGCTGCTGCTCAACCGCCGCCGGAAGGTTTTTCTGTTTCTCAGCATAATTCATTGTTTCATCATCAACAACAACAACAGCAGCAGCAACAACACCAGCACCAGCACCACCAGCAGCATCAACAGCAACAAGGCTCTATGGGTGAAGCTTCGGCGGCGAGGTTAGGGAATTATCTTCCTGGTCATCTGAATTTGTTGGCTTCATTGTCTGGTGGGAATGGAAATTCTGGTCGTGGAGATGACGAAGATCGTTGA
Protein sequence

>MsaT001567.1

MSNHKETTTITTTTDLVEPTSQNTLTVKKPPSKDRHSKVDGRGRRIRMPIICAARVFQLTRELGHKSDGQTIEWLLRQAEPSIIAATGTGTTPASFSSLSEHKPFLQPSPFILGKRLRDDDGDAVSVAAPTAPPGFWRPDFAQLWSFAAAAQPPPEGFSVSQHNSLFHHQQQQQQQQQHQHQHHQQHQQQQGSMGEASAARLGNYLPGHLNLLASLSGGNGNSGRGDDEDR*