AlfalfaGEDB | MsaG001676

M. sativa ssp. caerulea / MsaG001676

KEGG: K03083
GO: -
PFAM: PF00069.28

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG001676	MCI05308.1	84.483	58	4	1	2	59	12	64	1.35e-22	98.6

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG001676	sp\|P51139\|MSK3_MEDSA	84.483	58	4	1	2	59	237	289	4.04e-24	100

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG001676	tr\|A0A392NZV8\|A0A392NZV8_9FABA	84.483	58	4	1	2	59	12	64	6.44e-23	98.6

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsaG000325	MsaG001676	0.811436	1.202002e-50	2.125932e-48
MsaG001676	MsaG006769	0.810056	2.382222e-50	4.073658e-48
MsaG001676	MsaG008003	0.809403	3.285361e-50	5.530188e-48
MsaG001676	MsaG010651	0.837270	1.052053e-56	3.772948e-54
MsaG001676	MsaG017495	0.823499	2.368237e-53	5.718734e-51
MsaG001676	MsaG017642	0.826583	4.460887e-54	1.172464e-51
MsaG001676	MsaG020327	0.804594	3.383821e-49	5.081810e-47
MsaG001676	MsaG026754	0.837615	8.591811e-57	3.114130e-54
MsaG001676	MsaG028620	0.806841	1.147105e-49	1.815864e-47
MsaG001676	MsaG033214	0.828295	1.741720e-54	4.802366e-52
MsaG001676	MsaG041072	0.810580	1.838406e-50	3.184144e-48
MsaG001676	MsaG041299	0.810399	2.011090e-50	3.467981e-48
MsaG001676	MsaG043695	0.814068	3.210379e-51	6.062074e-49
MsaG001676	MsaG045331	0.819314	2.166985e-52	4.680631e-50
MsaG001676	MsaG045387	0.825225	9.343463e-54	2.364905e-51
MsaG001676	MsaG046306	0.809172	3.680690e-50	6.161170e-48
MsaG001676	MsaG046503	0.805638	2.050841e-49	3.155707e-47
MsaG001676	MsaG008811	0.827183	3.211923e-54	8.584297e-52
MsaG001676	MsaG013546	0.811897	9.551798e-51	1.708653e-48
MsaG001676	MsaG028049	0.811160	1.378797e-50	2.422218e-48
MsaG001676	MsaG031186	0.816144	1.116196e-51	2.221276e-49
MsaG001676	MsaG032445	0.801377	1.552809e-48	2.165795e-46
MsaG001676	MsaG037511	0.801841	1.248576e-48	1.759943e-46
MsaG001676	MsaG041727	0.806220	1.549226e-49	2.416845e-47
MsaG001676	MsaG042876	0.802030	1.142381e-48	1.617124e-46

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG001676	MtrunA17_Chr7g0270371	84.483	58	4	1	2	59	238	290	2.63e-25	100
MsaG001676	MtrunA17_Chr8g0350311	81.034	58	6	1	2	59	302	354	1.11e-24	99.4
MsaG001676	MtrunA17_Chr1g0193081	82.759	58	5	1	2	59	238	290	1.86e-24	98.2
MsaG001676	MtrunA17_Chr7g0246591	81.034	58	6	1	2	59	304	356	3.83e-24	97.8
MsaG001676	MtrunA17_Chr1g0194201	81.034	58	6	1	2	59	238	290	4.37e-24	97.1
MsaG001676	MtrunA17_Chr2g0277011	81.034	58	6	1	2	59	253	305	7.65e-24	96.7
MsaG001676	MtrunA17_Chr2g0320011	79.310	58	7	1	2	59	251	303	5.76e-23	94.4
MsaG001676	MtrunA17_Chr2g0284091	75.862	58	9	1	2	59	203	255	3.27e-22	91.7
MsaG001676	MtrunA17_Chr7g0248461	32.414	145	69	5	1	136	200	324	2.63e-12	63.9
MsaG001676	MtrunA17_Chr7g0273601	46.667	60	27	1	1	60	269	323	3.93e-12	63.5
MsaG001676	MtrunA17_Chr4g0002001	45.614	57	26	1	4	60	185	236	6.14e-12	62.8
MsaG001676	MtrunA17_Chr8g0348481	30.822	146	70	7	1	136	199	323	7.22e-12	62.8
MsaG001676	MtrunA17_Chr5g0396361	43.333	60	29	1	1	60	302	356	9.67e-12	62.4
MsaG001676	MtrunA17_Chr1g0191221	45.000	60	28	1	1	60	284	338	1.20e-11	62.4
MsaG001676	MtrunA17_Chr8g0383451	34.066	91	45	2	1	81	160	245	1.20e-11	62.0
MsaG001676	MtrunA17_Chr4g0051261	38.806	67	34	2	4	70	168	227	1.25e-11	61.6
MsaG001676	MtrunA17_Chr2g0286421	45.000	60	28	1	1	60	264	318	1.79e-11	61.6
MsaG001676	MtrunA17_Chr4g0066691	45.000	60	28	1	1	60	266	320	1.98e-11	61.6
MsaG001676	MtrunA17_Chr4g0030231	36.559	93	48	3	1	93	202	283	5.26e-11	60.1
MsaG001676	MtrunA17_Chr4g0030241	39.506	81	40	2	4	84	66	137	6.37e-11	58.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG001676	AT5G14640.1	84.483	58	4	1	2	59	237	289	5.75e-25	100
MsaG001676	AT5G14640.2	84.483	58	4	1	2	59	237	289	5.75e-25	100
MsaG001676	AT1G09840.11	79.310	58	7	1	2	59	119	171	4.00e-24	95.9
MsaG001676	AT3G05840.2	81.034	58	6	1	2	59	236	288	6.38e-24	97.1
MsaG001676	AT3G05840.1	81.034	58	6	1	2	59	236	288	6.38e-24	97.1
MsaG001676	AT5G26751.1	81.034	58	6	1	2	59	232	284	8.11e-24	96.7
MsaG001676	AT1G57870.1	81.034	58	6	1	2	59	245	297	8.33e-24	97.1
MsaG001676	AT1G57870.4	81.034	58	6	1	2	59	245	297	8.33e-24	97.1
MsaG001676	AT1G57870.2	81.034	58	6	1	2	59	245	297	8.33e-24	97.1
MsaG001676	AT1G57870.3	81.034	58	6	1	2	59	245	297	9.15e-24	97.1
MsaG001676	AT1G09840.3	79.310	58	7	1	2	59	246	298	2.24e-23	95.9
MsaG001676	AT1G09840.7	79.310	58	7	1	2	59	246	298	2.24e-23	95.9
MsaG001676	AT1G09840.10	79.310	58	7	1	2	59	246	298	2.24e-23	95.9
MsaG001676	AT1G09840.6	79.310	58	7	1	2	59	246	298	2.24e-23	95.9
MsaG001676	AT1G09840.4	79.310	58	7	1	2	59	246	298	2.24e-23	95.9
MsaG001676	AT1G09840.1	79.310	58	7	1	2	59	246	298	2.24e-23	95.9
MsaG001676	AT1G09840.9	79.310	58	7	1	2	59	246	298	2.24e-23	95.9
MsaG001676	AT1G09840.5	79.310	58	7	1	2	59	246	298	2.24e-23	95.9
MsaG001676	AT1G09840.8	79.310	58	7	1	2	59	246	298	2.24e-23	95.9
MsaG001676	AT1G09840.2	79.310	58	7	1	2	59	246	298	2.24e-23	95.9
MsaG001676	AT4G18710.2	77.586	58	8	1	2	59	197	249	4.49e-23	92.8
MsaG001676	AT2G30980.1	77.586	58	8	1	2	59	235	287	1.26e-22	93.6
MsaG001676	AT4G18710.1	77.586	58	8	1	2	59	203	255	1.30e-22	93.2
MsaG001676	AT1G06390.2	77.586	58	8	1	2	59	233	285	1.76e-22	93.2
MsaG001676	AT1G06390.1	77.586	58	8	1	2	59	233	285	1.76e-22	93.2
MsaG001676	AT4G00720.1	74.576	59	10	1	2	60	301	354	2.03e-22	93.2
MsaG001676	AT3G61160.5	72.414	58	11	1	2	59	265	317	1.53e-21	90.5
MsaG001676	AT3G61160.3	72.414	58	11	1	2	59	265	317	1.63e-21	89.7
MsaG001676	AT3G61160.6	72.414	58	11	1	2	59	272	324	2.01e-21	89.7
MsaG001676	AT3G61160.1	72.414	58	11	1	2	59	265	317	2.04e-21	90.5
MsaG001676	AT3G61160.2	72.414	58	11	1	2	59	272	324	2.42e-21	90.1
MsaG001676	AT3G61160.4	72.414	58	11	1	2	59	265	317	2.71e-21	90.1
MsaG001676	AT2G46070.1	31.944	144	73	5	1	137	203	328	6.98e-14	68.9
MsaG001676	AT2G46070.3	31.944	144	73	5	1	137	237	362	7.65e-14	68.9
MsaG001676	AT4G10010.1	52.632	57	22	1	4	60	141	192	1.23e-13	68.2
MsaG001676	AT4G10010.2	50.000	60	25	1	1	60	318	372	3.29e-13	67.0
MsaG001676	AT2G38620.3	43.333	60	29	1	1	60	182	236	5.18e-13	65.5
MsaG001676	AT2G38620.1	43.333	60	29	1	1	60	182	236	5.18e-13	65.5
MsaG001676	AT2G46070.2	53.704	54	20	1	1	54	203	251	6.86e-13	65.9
MsaG001676	AT1G07880.2	33.103	145	68	7	1	136	195	319	8.01e-13	65.9
MsaG001676	AT5G63370.9	53.704	54	20	1	4	57	332	380	1.02e-12	65.5
MsaG001676	AT5G63370.2	53.704	54	20	1	4	57	332	380	1.06e-12	65.5
MsaG001676	AT5G63370.3	53.704	54	20	1	4	57	332	380	1.06e-12	65.5
MsaG001676	AT2G38620.4	45.614	57	26	1	4	60	123	174	1.98e-12	63.9
MsaG001676	AT5G63370.5	50.877	57	23	1	1	57	463	514	2.15e-12	64.7
MsaG001676	AT5G63370.10	50.877	57	23	1	1	57	463	514	2.15e-12	64.7
MsaG001676	AT5G63370.7	50.877	57	23	1	1	57	463	514	2.20e-12	64.7
MsaG001676	AT5G63370.8	50.877	57	23	1	1	57	463	514	2.20e-12	64.7
MsaG001676	AT5G63370.4	50.877	57	23	1	1	57	463	514	2.20e-12	64.7
MsaG001676	AT5G63370.6	50.877	57	23	1	1	57	463	514	2.20e-12	64.7
MsaG001676	AT5G63370.1	50.877	57	23	1	1	57	463	514	2.20e-12	64.7
MsaG001676	AT2G38620.2	45.614	57	26	1	4	60	185	236	2.64e-12	63.9
MsaG001676	AT3G48750.1	46.429	56	25	1	4	59	168	218	4.13e-12	63.5
MsaG001676	AT1G01560.2	29.167	144	89	4	1	143	202	333	4.82e-12	63.5
MsaG001676	AT1G71530.2	47.368	57	25	1	4	60	312	363	1.10e-11	62.8
MsaG001676	AT1G76540.1	43.333	60	29	1	1	60	183	237	1.15e-11	62.4
MsaG001676	AT1G71530.3	47.368	57	25	1	4	60	312	363	1.35e-11	62.4
MsaG001676	AT4G01370.1	30.657	137	82	4	1	136	205	329	1.58e-11	62.0
MsaG001676	AT1G54610.1	46.667	60	27	1	1	60	280	334	1.58e-11	62.4
MsaG001676	AT1G54610.3	46.667	60	27	1	1	60	280	334	1.58e-11	62.4
MsaG001676	AT1G54610.2	46.667	60	27	1	1	60	280	334	1.58e-11	62.4
MsaG001676	AT3G54180.1	43.860	57	27	1	4	60	183	234	2.13e-11	61.6
MsaG001676	AT1G71530.1	45.000	60	28	1	1	60	309	363	2.89e-11	61.6
MsaG001676	AT1G01560.4	47.541	61	27	1	4	64	205	260	3.24e-11	60.8
MsaG001676	AT1G01560.1	47.541	61	27	1	4	64	205	260	3.24e-11	60.8
MsaG001676	AT1G01560.3	50.000	54	22	1	1	54	202	250	4.67e-11	60.5
MsaG001676	AT1G20930.1	41.667	60	30	1	1	60	185	239	5.94e-11	60.1

Find 33 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTTAAAGTTCAGGCAGTTAT+TGG	0.206518	1:+24303522	MsaT001676.1:CDS
TCTTATCAGTCACTGTTTCC+TGG	0.318560	1:+24301956	MsaT001676.1:intron
TGAGATTTACTCTCCTTCTA+AGG	0.373862	1:+24303629	MsaT001676.1:CDS
AGATAAACAATGCGCTCATT+TGG	0.387618	1:+24303340	MsaT001676.1:CDS
TGGTCAGTTGGTTGCGTACT+TGG	0.388547	1:+24301838	MsaT001676.1:CDS
ATTGATACTGAGAAGATTGT+TGG	0.390696	1:+24303764	MsaT001676.1:CDS
CTTTGTATGATGGTTCCTAT+AGG	0.404739	1:+24303553	MsaT001676.1:CDS
GTACACAAAAGCTAGTTAAC+TGG	0.433946	1:-24301876	None:intergenic
GGACCCCATGCTTTGTATGA+TGG	0.435370	1:+24303543	MsaT001676.1:CDS
ACAAACCCTTCTCACCCTAT+AGG	0.443996	1:-24303568	None:intergenic
CCTTGATAATTTCCACAAGT+TGG	0.452229	1:-24301994	None:intergenic
CTTTGGAAGTGCTAATGTCT+TGG	0.461224	1:+24301628	None:intergenic
CATCAGTTTCATCAGATGCT+TGG	0.473349	1:-24303274	None:intergenic
AGAGCTCCTGAGCTTATATT+CGG	0.474562	1:+24301784	MsaT001676.1:CDS
TTTGTATGATGGTTCCTATA+GGG	0.492577	1:+24303554	MsaT001676.1:CDS
TCATTTGGTTGCTGTAGCTA+AGG	0.499648	1:+24303355	MsaT001676.1:CDS
CCAACTTGTGGAAATTATCA+AGG	0.511188	1:+24301994	MsaT001676.1:CDS
AGGAACCATCATACAAAGCA+TGG	0.519567	1:-24303548	None:intergenic
ACACCACAACCATTGACATC+TGG	0.523107	1:+24301818	MsaT001676.1:CDS
TCACTGTTTCCTGGTGAGAG+TGG	0.525574	1:+24301965	MsaT001676.1:CDS
ACCATTGACATCTGGTCAGT+TGG	0.528063	1:+24301826	MsaT001676.1:CDS
TACTGAGAAGATTGTTGGCT+CGG	0.541038	1:+24303769	MsaT001676.1:CDS
ATGGTTCCTATAGGGTGAGA+AGG	0.558535	1:+24303562	MsaT001676.1:CDS
GGAACCATCATACAAAGCAT+GGG	0.561104	1:-24303547	None:intergenic
TGACCAGATGTCAATGGTTG+TGG	0.568382	1:-24301821	None:intergenic
GTTGCACCGAATATAAGCTC+AGG	0.582148	1:-24301790	None:intergenic
TGGTCTACTCCACTCTCACC+AGG	0.583110	1:-24301974	None:intergenic
GGTTGATGCTTCGCAGACTG+AGG	0.585844	1:+24303790	MsaT001676.1:CDS
ACCAACTGACCAGATGTCAA+TGG	0.588620	1:-24301827	None:intergenic
TGGTTCCTATAGGGTGAGAA+GGG	0.594607	1:+24303563	MsaT001676.1:CDS
GAATGTTTCACTTGATGATG+AGG	0.600116	1:+24303739	MsaT001676.1:CDS
GAGTGGAGTAGACCAACTTG+TGG	0.666033	1:+24301982	MsaT001676.1:CDS
GAACCATCATACAAAGCATG+GGG	0.696724	1:-24303546	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content

Chromosome	Type	Strat	End	Strand	Name
Chr1	gene	24301642	24303826	24301642	ID=MsaG001676
Chr1	mRNA	24301642	24303826	24301642	ID=MsaT001676.1;Parent=MsaG001676
Chr1	exon	24301642	24301649	24301642	ID=MsaT001676.1.exon1;Parent=MsaT001676.1
Chr1	CDS	24301642	24301649	24301642	ID=cds.MsaT001676.1;Parent=MsaT001676.1
Chr1	exon	24301777	24301894	24301777	ID=MsaT001676.1.exon2;Parent=MsaT001676.1
Chr1	CDS	24301777	24301894	24301777	ID=cds.MsaT001676.1;Parent=MsaT001676.1
Chr1	exon	24301965	24302015	24301965	ID=MsaT001676.1.exon3;Parent=MsaT001676.1
Chr1	CDS	24301965	24302015	24301965	ID=cds.MsaT001676.1;Parent=MsaT001676.1
Chr1	exon	24303260	24303376	24303260	ID=MsaT001676.1.exon4;Parent=MsaT001676.1
Chr1	CDS	24303260	24303376	24303260	ID=cds.MsaT001676.1;Parent=MsaT001676.1
Chr1	exon	24303507	24303650	24303507	ID=MsaT001676.1.exon5;Parent=MsaT001676.1
Chr1	CDS	24303507	24303650	24303507	ID=cds.MsaT001676.1;Parent=MsaT001676.1
Chr1	exon	24303734	24303826	24303734	ID=MsaT001676.1.exon6;Parent=MsaT001676.1
Chr1	CDS	24303734	24303826	24303734	ID=cds.MsaT001676.1;Parent=MsaT001676.1

Gene Sequence

>MsaT001676.1

ATGTCTTGGTACTATAGAGCTCCTGAGCTTATATTCGGTGCAACTGAGTACACCACAACCATTGACATCTGGTCAGTTGGTTGCGTACTTGGTGAACTTTTGCTTGGCCAGTTAACTAGCTTTTGTTCACTGTTTCCTGGTGAGAGTGGAGTAGACCAACTTGTGGAAATTATCAAGTTTAAGGTTAAACTCCAAGCATCTGATGAAACTGATGATGCAGTGTTTGTTGCTTTTGACACTGACATGGGTTTTCTTCTAGATAAACAATGCGCTCATTTGGTTGCTGTAGCTAAGGGGAAGAAGATGCTTTTTAAAGTTCAGGCAGTTATTGGACCCCATGCTTTGTATGATGGTTCCTATAGGGTGAGAAGGGTTTGTATTGATGAAGCTATTATTGAATTGTTTCTCACTGATGATGAGATTTACTCTCCTTCTAAGGCGTTGAATGTTTCACTTGATGATGAGGTTATTGATACTGAGAAGATTGTTGGCTCGGTTGATGCTTCGCAGACTGAGGATTTTGTCTGTTAA

Protein sequence

>MsaT001676.1

MSWYYRAPELIFGATEYTTTIDIWSVGCVLGELLLGQLTSFCSLFPGESGVDQLVEIIKFKVKLQASDETDDAVFVAFDTDMGFLLDKQCAHLVAVAKGKKMLFKVQAVIGPHALYDGSYRVRRVCIDEAIIELFLTDDEIYSPSKALNVSLDDEVIDTEKIVGSVDASQTEDFVC*