AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG001709


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG001709 MtrunA17_Chr1g0166461 91.071 168 12 2 1 166 1 167 4.29e-111 313
MsaG001709 MtrunA17_Chr1g0170601 59.036 166 55 4 1 166 1 153 3.70e-58 178
MsaG001709 MtrunA17_Chr5g0423491 59.091 176 44 5 1 166 1 158 3.32e-53 166
MsaG001709 MtrunA17_Chr1g0187511 35.443 158 84 6 11 166 4 145 3.00e-15 68.9
MsaG001709 MtrunA17_Chr1g0187541 33.129 163 74 7 14 166 9 146 1.36e-13 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 35 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATTGGTAAACATGTGAATT+TGG 0.166169 1:-25116480 None:intergenic
GAAAATTGATTTCATTGATT+TGG 0.219457 1:+25116575 MsaT001709.1:CDS
TAGAACCAACATTTAGAAAA+TGG 0.241260 1:-25116434 None:intergenic
TTTAGAAAATGGTTTCTTCT+TGG 0.277705 1:-25116423 None:intergenic
ATTGCACTTGAGGATTGATA+TGG 0.362335 1:-25116314 None:intergenic
ACTCAAAACATCTATGATAA+TGG 0.384781 1:+25116381 MsaT001709.1:CDS
CAACTCGGGTGCTCAAACAT+TGG 0.437124 1:+25116614 MsaT001709.1:CDS
AAATGGTTTCTTCTTGGTTG+TGG 0.446475 1:-25116417 None:intergenic
ATTGATTTCATTGATTTGGT+TGG 0.453682 1:+25116579 MsaT001709.1:CDS
CCTTCTCATTGTACCCCGCC+AGG 0.480018 1:+25116339 MsaT001709.1:CDS
CTTCTTGTAGATGCTTCCAT+TGG 0.494962 1:-25116497 None:intergenic
CAAGAAGAATCTTCAAATGA+TGG 0.496730 1:+25116513 MsaT001709.1:CDS
ATCTTCAAATGATGGTACTA+AGG 0.499653 1:+25116521 MsaT001709.1:CDS
GGTTTCTTCTTGGTTGTGGT+TGG 0.536103 1:-25116413 None:intergenic
TTGGAACATGAAAACCTGGC+GGG 0.537488 1:-25116353 None:intergenic
TGAGAAGGAAATTGCACTTG+AGG 0.547978 1:-25116324 None:intergenic
CCTCGTGTTACTAAAGCCAT+TGG 0.551040 1:-25116160 None:intergenic
CAAATTCACATGTTTACCAA+TGG 0.564592 1:+25116481 MsaT001709.1:CDS
AAAACATCTATGATAATGGT+AGG 0.569456 1:+25116385 MsaT001709.1:CDS
GTTACTAAAGCCATTGGTCA+TGG 0.570356 1:-25116154 None:intergenic
CTTGAGTGCTTATGAAAACT+CGG 0.574814 1:-25116221 None:intergenic
TCAATTGGAACATGAAAACC+TGG 0.579474 1:-25116357 None:intergenic
AACTCATATCGAGTCTCACA+TGG 0.585317 1:+25116251 MsaT001709.1:CDS
TTCACCACCACCATGACCAA+TGG 0.588746 1:+25116144 None:intergenic
TCTATGCAACTTACACCACA+TGG 0.620484 1:+25116453 MsaT001709.1:CDS
CCTGGCGGGGTACAATGAGA+AGG 0.622633 1:-25116339 None:intergenic
TGGTAATGATGATGACAACT+CGG 0.654905 1:+25116599 MsaT001709.1:CDS
GATAAGAAGACTCTATTCGA+CGG 0.664525 1:+25116290 MsaT001709.1:CDS
TGGAACATGAAAACCTGGCG+GGG 0.669917 1:-25116352 None:intergenic
GAGGCATGATGTGTTAAACT+CGG 0.676388 1:+25116179 MsaT001709.1:CDS
CCAATGGCTTTAGTAACACG+AGG 0.678872 1:+25116160 MsaT001709.1:CDS
ATTGGAACATGAAAACCTGG+CGG 0.713059 1:-25116354 None:intergenic
GAGTCTCACATGGAGAACGA+AGG 0.725705 1:+25116261 MsaT001709.1:CDS
GGTAATGATGATGACAACTC+GGG 0.729679 1:+25116600 MsaT001709.1:CDS
TGTGAATTTGGCAAACCATG+TGG 0.742651 1:-25116468 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr1 gene 25116156 25116656 25116156 ID=MsaG001709
Chr1 mRNA 25116156 25116656 25116156 ID=MsaT001709.1;Parent=MsaG001709
Chr1 exon 25116156 25116656 25116156 ID=MsaT001709.1.exon1;Parent=MsaT001709.1
Chr1 CDS 25116156 25116656 25116156 ID=cds.MsaT001709.1;Parent=MsaT001709.1
Gene Sequence

>MsaT001709.1

ATGACCAATGGCTTTAGTAACACGAGGCATGATGTGTTAAACTCGGTTGCATGTAGATTATGCAACCGAGTTTTCATAAGCACTCAAGCACTCATAACTCATATCGAGTCTCACATGGAGAACGAAGGTGCTGCGATAAGAAGACTCTATTCGACGGACCATATCAATCCTCAAGTGCAATTTCCTTCTCATTGTACCCCGCCAGGTTTTCATGTTCCAATTGACACTCAAAACATCTATGATAATGGTAGGATTTTCCAACCACAACCAAGAAGAAACCATTTTCTAAATGTTGGTTCTATGCAACTTACACCACATGGTTTGCCAAATTCACATGTTTACCAATGGAAGCATCTACAAGAAGAATCTTCAAATGATGGTACTAAGGCATATATCATGCAGCTTGAGAAACCTATCAAGAAAATTGATTTCATTGATTTGGTTGGTAATGATGATGACAACTCGGGTGCTCAAACATTGGATTTAGCACTTAAGCTCTAG

Protein sequence

>MsaT001709.1

MTNGFSNTRHDVLNSVACRLCNRVFISTQALITHIESHMENEGAAIRRLYSTDHINPQVQFPSHCTPPGFHVPIDTQNIYDNGRIFQPQPRRNHFLNVGSMQLTPHGLPNSHVYQWKHLQEESSNDGTKAYIMQLEKPIKKIDFIDLVGNDDDNSGAQTLDLALKL*