Alfalfa Gene Editing Database
Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|---|---|---|---|---|---|---|---|---|---|---|
MsaG001719 | AES91985.1 | 63.636 | 66 | 23 | 1 | 1 | 66 | 24 | 88 | 5.34e-16 | 76.6 |
Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|---|---|---|---|---|---|---|---|---|---|---|
MsaG001719 | sp|Q84TQ7|GAI_GOSHI | 57.971 | 69 | 28 | 1 | 1 | 68 | 162 | 230 | 4.65e-18 | 79.3 |
Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|---|---|---|---|---|---|---|---|---|---|---|
MsaG001719 | tr|G7JNX8|G7JNX8_MEDTR | 63.636 | 66 | 23 | 1 | 1 | 66 | 24 | 88 | 2.55e-16 | 76.6 |
Gene ID | Type | Classification |
---|---|---|
MsaG001719 | TF | GRAS |
Gene ID | Type | Classification |
---|
Co-expression Network
Gene1 | Gene2 | correlation coefficient | p_value | FDR |
---|
PPI
Gene1 | Gene2 | Type |
---|
Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|---|---|---|---|---|---|---|---|---|---|---|
MsaG001719 | MtrunA17_Chr4g0068471 | 63.636 | 66 | 23 | 1 | 1 | 66 | 24 | 88 | 4.91e-20 | 76.6 |
MsaG001719 | MtrunA17_Chr3g0110971 | 61.290 | 62 | 24 | 0 | 1 | 62 | 207 | 268 | 7.99e-19 | 78.2 |
MsaG001719 | MtrunA17_Chr8g0376381 | 50.000 | 56 | 28 | 0 | 7 | 62 | 163 | 218 | 1.33e-12 | 60.5 |
Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|---|---|---|---|---|---|---|---|---|---|---|
MsaG001719 | AT2G01570.1 | 52.239 | 67 | 32 | 0 | 1 | 67 | 212 | 278 | 1.53e-15 | 69.3 |
MsaG001719 | AT1G66350.1 | 51.613 | 62 | 30 | 0 | 1 | 62 | 143 | 204 | 2.98e-15 | 68.6 |
MsaG001719 | AT5G17490.1 | 53.125 | 64 | 30 | 0 | 4 | 67 | 151 | 214 | 2.98e-15 | 68.6 |
MsaG001719 | AT1G14920.1 | 49.254 | 67 | 34 | 0 | 1 | 67 | 160 | 226 | 3.81e-15 | 68.2 |
MsaG001719 | AT3G03450.1 | 51.613 | 62 | 30 | 0 | 1 | 62 | 171 | 232 | 8.92e-15 | 67.0 |
Find 9 sgRNAs with CRISPR-Local
Find sgRNAs with CRISPR-GE
CRISPR-Local
sgRNA_sequence | on_target_score | Position | Region |
---|---|---|---|
AGTTCAACCCCTATCGCCTC+TGG | 0.395762 | 1:-25261440 | None:intergenic |
CCTCTATGCTAAAATTAGTC+AGG | 0.453663 | 1:-25261632 | None:intergenic |
CCCTATCGCCTCTGGGTCCA+TGG | 0.457846 | 1:-25261432 | None:intergenic |
CCATGGACCCAGAGGCGATA+GGG | 0.457994 | 1:+25261432 | None:intergenic |
CGAGCATAATATTCTCTCGC+TGG | 0.479992 | 1:-25261496 | None:intergenic |
CATGGACCCAGAGGCGATAG+GGG | 0.566921 | 1:+25261433 | None:intergenic |
GTTCAACCCCTATCGCCTCT+GGG | 0.580458 | 1:-25261439 | None:intergenic |
TCCATGGACCCAGAGGCGAT+AGG | 0.603401 | 1:+25261431 | None:intergenic |
ACTGATTTCCATGGACCCAG+AGG | 0.699900 | 1:+25261424 | None:intergenic |
CRISPR-GE
badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
---|
Chromosome | Type | Strat | End | Strand | Name |
---|---|---|---|---|---|
Chr1 | gene | 25261434 | 25261640 | 25261434 | ID=MsaG001719 |
Chr1 | mRNA | 25261434 | 25261640 | 25261434 | ID=MsaT001719.1;Parent=MsaG001719 |
Chr1 | exon | 25261434 | 25261640 | 25261434 | ID=MsaT001719.1.exon1;Parent=MsaT001719.1 |
Chr1 | CDS | 25261434 | 25261640 | 25261434 | ID=cds.MsaT001719.1;Parent=MsaT001719.1 |
Gene Sequence |
Protein sequence |