AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG001725

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG001725 MtrunA17_Chr1g0166681 86.869 198 26 0 1 198 1 198 2.07e-108 308
MsaG001725 MtrunA17_Chr5g0401061 67.416 89 27 1 1 87 1 89 1.12e-34 124
MsaG001725 MtrunA17_Chr4g0054131 65.169 89 30 1 1 88 1 89 2.42e-33 120
MsaG001725 MtrunA17_Chr8g0384671 79.310 58 12 0 1 58 1 58 4.18e-27 104
MsaG001725 MtrunA17_Chr2g0293381 72.131 61 17 0 1 61 1 61 1.51e-26 102
MsaG001725 MtrunA17_Chr8g0383821 71.930 57 16 0 1 57 1 57 2.94e-25 95.5
MsaG001725 MtrunA17_Chr2g0329921 67.857 56 18 0 1 56 1 56 7.22e-21 87.8
MsaG001725 MtrunA17_Chr8g0342871 60.345 58 23 0 1 58 1 58 1.03e-18 81.6
MsaG001725 MtrunA17_Chr1g0195261 70.000 50 15 0 1 50 1 50 1.37e-16 70.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG001725 AT5G67580.2 63.529 85 29 1 1 83 1 85 6.89e-29 109
MsaG001725 AT5G67580.1 63.529 85 29 1 1 83 1 85 6.89e-29 109
MsaG001725 AT3G49850.1 68.657 67 21 0 1 67 1 67 4.41e-27 104
MsaG001725 AT3G49850.2 68.657 67 21 0 1 67 1 67 4.41e-27 104
MsaG001725 AT1G49950.3 74.138 58 15 0 1 58 1 58 1.14e-25 100
MsaG001725 AT1G49950.1 74.138 58 15 0 1 58 1 58 1.14e-25 100
MsaG001725 AT1G49950.2 74.138 58 15 0 1 58 1 58 1.14e-25 100
MsaG001725 AT1G72740.2 59.375 64 26 0 1 64 1 64 5.08e-20 85.5
MsaG001725 AT1G72740.1 63.793 58 21 0 1 58 1 58 5.25e-20 85.5
MsaG001725 AT1G17520.3 57.576 66 28 0 1 66 1 66 8.89e-20 85.1
MsaG001725 AT1G17520.4 57.576 66 28 0 1 66 1 66 9.33e-20 85.1
MsaG001725 AT1G17520.1 57.576 66 28 0 1 66 1 66 1.01e-19 85.1
MsaG001725 AT1G17520.2 57.576 66 28 0 1 66 1 66 1.02e-19 85.1

Find 28 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGCAAACTAGTGTTGAATTC+TGG 0.175108 1:+25335521 None:intergenic
TCATTTCTCGCAGTTGCTTT+TGG 0.254917 1:+25335290 None:intergenic
GGCTGAGGAAGCAACTTATA+AGG 0.388838 1:-25335135 MsaT001725.1:CDS
GCAAACTAGTGTTGAATTCT+GGG 0.405933 1:+25335522 None:intergenic
TAAGGGTCTCACACCTCAAC+AGG 0.416868 1:-25335210 MsaT001725.1:CDS
CAGAGCTGCCTCTTCTTCTC+CGG 0.417460 1:+25335598 None:intergenic
GAATATCAATATTCGATCAT+AGG 0.430998 1:-25335361 MsaT001725.1:CDS
ATGTAGACCTCAAGGACAAA+TGG 0.452440 1:-25335487 MsaT001725.1:CDS
GAAGAGGCAGCTCTGAAAGC+GGG 0.486977 1:-25335590 MsaT001725.1:CDS
GGCAGAAGCTGCCATGACTA+AGG 0.496803 1:-25335156 MsaT001725.1:CDS
GGAGTAGTCAAACACGGAGC+TGG 0.501232 1:-25335569 MsaT001725.1:CDS
AGTTCCTCCATTTGTCCTTG+AGG 0.505844 1:+25335480 None:intergenic
TCAAACACGGAGCTGGAAGA+TGG 0.524401 1:-25335562 MsaT001725.1:CDS
CGACGACAACATTTAGATCA+AGG 0.524618 1:-25335451 MsaT001725.1:CDS
AATAATAATCAATATTCAAG+TGG 0.553519 1:-25335383 MsaT001725.1:CDS
AAAATGGACCGGAGAAGAAG+AGG 0.564638 1:-25335606 MsaT001725.1:CDS
AGCTGCAAAAGCAATGGAGG+AGG 0.570145 1:-25335177 MsaT001725.1:CDS
AGCTGCCATGACTAAGGCTG+AGG 0.573794 1:-25335150 MsaT001725.1:CDS
GGAAGCAACTTATAAGGCAG+AGG 0.583297 1:-25335129 MsaT001725.1:CDS
TGCTTCCTCAGCCTTAGTCA+TGG 0.584066 1:+25335145 None:intergenic
AGAAGAAGCTGCAAAAGCAA+TGG 0.585302 1:-25335183 MsaT001725.1:CDS
TGCAAAAGCAGAAATGAAGA+AGG 0.586897 1:-25335072 MsaT001725.1:CDS
GCTTCTTCTATCGCCTGTTG+AGG 0.590627 1:+25335197 None:intergenic
AGAAGCTGCAAAAGCAATGG+AGG 0.603836 1:-25335180 MsaT001725.1:CDS
TCGTTCAAATGTAGACCTCA+AGG 0.605301 1:-25335495 MsaT001725.1:CDS
AGAAGAGGCAGCTCTGAAAG+CGG 0.618500 1:-25335591 MsaT001725.1:CDS
TAGACCTCAAGGACAAATGG+AGG 0.657376 1:-25335484 MsaT001725.1:CDS
AAAGCGGGAGTAGTCAAACA+CGG 0.721145 1:-25335575 MsaT001725.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr1 gene 25335049 25335645 25335049 ID=MsaG001725
Chr1 mRNA 25335049 25335645 25335049 ID=MsaT001725.1;Parent=MsaG001725
Chr1 exon 25335049 25335645 25335049 ID=MsaT001725.1.exon1;Parent=MsaT001725.1
Chr1 CDS 25335049 25335645 25335049 ID=cds.MsaT001725.1;Parent=MsaT001725.1
Gene Sequence

>MsaT001725.1

ATGGGTGCTCCTAAGCAAAAATGGACCGGAGAAGAAGAGGCAGCTCTGAAAGCGGGAGTAGTCAAACACGGAGCTGGAAGATGGCGCGCTATACTTAAAGACCCAGAATTCAACACTAGTTTGCGCAATCGTTCAAATGTAGACCTCAAGGACAAATGGAGGAACTTAACTACGACGACAACATTTAGATCAAGGAAGAAGACAGAAAACATTTTGCTTCTACCTCCACCTAAAGTTGAAAATAATAATCAATATTCAAGTGGAATATCAATATTCGATCATAGGATTGCCTCAACTTCAACAAAAATACGCGAGGAAACAAGAAATGATTCTCCAAAAGCAACTGCGAGAAATGATGACGTCAACTTCCTTTCAAAATACGAAGAAATTAATGAAGAGATTTTTAAAATGATTAAGGGTCTCACACCTCAACAGGCGATAGAAGAAGCTGCAAAAGCAATGGAGGAGGCAGAAGCTGCCATGACTAAGGCTGAGGAAGCAACTTATAAGGCAGAGGCTGCTGAAGCTGAAGCAGAGAATGCACGATTGTTTGCAAAAGCAGAAATGAAGAAGGCACTAAAAATGCTCAAGATTTAA
Protein sequence

>MsaT001725.1

MGAPKQKWTGEEEAALKAGVVKHGAGRWRAILKDPEFNTSLRNRSNVDLKDKWRNLTTTTTFRSRKKTENILLLPPPKVENNNQYSSGISIFDHRIASTSTKIREETRNDSPKATARNDDVNFLSKYEEINEEIFKMIKGLTPQQAIEEAAKAMEEAEAAMTKAEEATYKAEAAEAEAENARLFAKAEMKKALKMLKI*