AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG001756


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG001756 MtrunA17_Chr1g0187371 89.247 93 10 0 1 93 1 93 2.27e-51 163
MsaG001756 MtrunA17_Chr4g0002001 67.143 70 23 0 18 87 4 73 1.26e-25 97.1
MsaG001756 MtrunA17_Chr4g0051261 53.731 67 30 1 18 84 4 69 6.91e-18 76.3
MsaG001756 MtrunA17_Chr2g0293491 52.239 67 31 1 18 84 4 69 4.49e-17 73.9
MsaG001756 MtrunA17_Chr3g0093571 52.239 67 31 1 18 84 25 90 1.56e-15 70.5
MsaG001756 MtrunA17_Chr3g0093391 50.746 67 32 1 18 84 23 88 2.00e-15 70.1
MsaG001756 MtrunA17_Chr4g0061141 50.769 65 31 1 17 81 104 167 2.47e-12 61.2
MsaG001756 MtrunA17_Chr8g0385971 50.000 64 31 1 18 81 90 152 3.22e-12 60.8
MsaG001756 MtrunA17_Chr2g0286421 47.436 78 39 2 5 81 88 164 4.97e-12 60.5
MsaG001756 MtrunA17_Chr7g0218071 48.718 78 38 2 5 81 106 182 5.18e-12 60.5
MsaG001756 MtrunA17_Chr4g0057791 49.123 57 29 0 18 74 218 274 1.19e-11 59.3
MsaG001756 MtrunA17_Chr4g0066691 47.692 65 33 1 17 81 103 166 1.84e-11 58.9
MsaG001756 MtrunA17_Chr3g0131861 48.485 66 33 1 16 81 95 159 2.56e-11 58.5
MsaG001756 MtrunA17_Chr6g0453711 46.154 65 34 1 17 81 109 172 4.36e-11 57.8
MsaG001756 MtrunA17_Chr1g0191221 48.438 64 32 1 18 81 122 184 4.48e-11 57.8
MsaG001756 MtrunA17_Chr2g0317501 49.231 65 32 1 17 81 133 196 6.58e-11 57.4
MsaG001756 MtrunA17_Chr7g0273601 46.875 64 33 1 18 81 107 169 9.63e-11 57.0
MsaG001756 MtrunA17_Chr1g0201571 40.506 79 45 2 4 81 111 188 9.85e-11 56.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG001756 AT1G76540.1 77.381 84 19 0 10 93 6 89 5.88e-39 132
MsaG001756 AT1G20930.1 78.049 82 18 0 12 93 10 91 1.79e-38 130
MsaG001756 AT2G38620.3 61.250 80 29 1 18 97 4 81 1.00e-25 96.7
MsaG001756 AT2G38620.1 61.250 80 29 1 18 97 4 81 1.00e-25 96.7
MsaG001756 AT2G38620.2 61.250 80 29 1 18 97 4 81 3.37e-25 96.3
MsaG001756 AT3G54180.1 66.197 71 24 0 18 88 4 74 1.77e-24 94.4
MsaG001756 AT5G10270.1 48.052 77 36 1 18 94 26 98 3.93e-17 75.5
MsaG001756 AT5G64960.1 48.052 77 36 1 18 94 26 98 4.43e-17 75.1
MsaG001756 AT3G48750.1 52.239 67 31 1 18 84 4 69 5.15e-17 74.3
MsaG001756 AT1G67580.1 50.000 70 31 2 18 87 406 471 1.42e-13 65.5
MsaG001756 AT1G67580.2 50.000 70 31 2 18 87 406 471 1.42e-13 65.5
MsaG001756 AT1G18670.2 52.308 65 30 1 17 81 130 193 2.50e-13 64.7
MsaG001756 AT1G18670.3 52.308 65 30 1 17 81 130 193 3.18e-13 64.3
MsaG001756 AT1G18670.1 52.308 65 30 1 17 81 130 193 3.47e-13 64.3
MsaG001756 AT1G71530.4 50.000 66 32 1 16 81 145 209 2.36e-12 61.6
MsaG001756 AT1G71530.3 50.000 66 32 1 16 81 145 209 2.73e-12 61.6
MsaG001756 AT4G10010.2 47.436 78 39 2 5 81 142 218 2.88e-12 61.6
MsaG001756 AT1G71530.2 50.000 66 32 1 16 81 145 209 3.44e-12 61.2
MsaG001756 AT1G74330.3 50.769 65 31 1 17 81 120 183 3.60e-12 61.2
MsaG001756 AT1G66750.1 51.667 60 28 1 24 83 19 77 3.74e-12 61.2
MsaG001756 AT1G33770.1 50.769 65 31 1 17 81 140 203 4.06e-12 61.2
MsaG001756 AT1G74330.1 50.769 65 31 1 17 81 120 183 4.31e-12 61.2
MsaG001756 AT1G74330.2 50.769 65 31 1 17 81 120 183 4.39e-12 61.2
MsaG001756 AT1G71530.1 50.000 66 32 1 16 81 145 209 9.72e-12 60.1
MsaG001756 AT3G05050.3 43.590 78 42 2 5 81 124 200 9.73e-12 60.1
MsaG001756 AT3G05050.2 43.590 78 42 2 5 81 124 200 9.73e-12 60.1
MsaG001756 AT3G05050.1 43.590 78 42 2 5 81 124 200 9.73e-12 60.1
MsaG001756 AT5G39420.1 47.143 70 28 3 16 81 103 167 1.13e-11 59.7
MsaG001756 AT2G38620.4 74.359 39 10 0 18 56 4 42 1.92e-11 58.5
MsaG001756 AT1G57700.1 50.769 65 31 1 17 81 145 208 2.23e-11 58.9
MsaG001756 AT1G03740.2 50.000 64 31 1 18 81 213 275 3.53e-11 58.5
MsaG001756 AT1G09600.1 49.231 65 32 1 17 81 162 225 3.78e-11 58.5
MsaG001756 AT5G63370.5 41.860 86 44 3 4 84 282 366 3.92e-11 58.2
MsaG001756 AT5G63370.10 41.860 86 44 3 4 84 282 366 3.92e-11 58.2
MsaG001756 AT5G63370.7 41.860 86 44 3 4 84 282 366 4.91e-11 58.2
MsaG001756 AT5G63370.8 41.860 86 44 3 4 84 282 366 4.91e-11 58.2
MsaG001756 AT5G63370.4 41.860 86 44 3 4 84 282 366 4.91e-11 58.2
MsaG001756 AT5G63370.6 41.860 86 44 3 4 84 282 366 4.91e-11 58.2
MsaG001756 AT5G63370.1 41.860 86 44 3 4 84 282 366 4.91e-11 58.2
MsaG001756 AT5G50860.1 44.444 72 37 2 12 81 106 176 5.23e-11 58.2
MsaG001756 AT1G03740.1 50.000 64 31 1 18 81 213 275 5.77e-11 57.8
MsaG001756 AT5G63370.9 45.070 71 34 2 18 84 163 232 6.80e-11 57.8
MsaG001756 AT5G63370.2 45.070 71 34 2 18 84 163 232 7.65e-11 57.4
MsaG001756 AT5G63370.3 45.070 71 34 2 18 84 163 232 7.65e-11 57.4
MsaG001756 AT1G54610.1 48.438 64 32 1 18 81 118 180 8.26e-11 57.4
MsaG001756 AT1G54610.3 48.438 64 32 1 18 81 118 180 8.26e-11 57.4
MsaG001756 AT1G54610.2 48.438 64 32 1 18 81 118 180 8.26e-11 57.4

Find 20 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGGGATAACTGTGTTGTATT+TGG 0.270067 1:-25845436 MsaT001756.1:CDS
TGTTGGATGTGAAACAATTA+AGG 0.332045 1:-25845471 MsaT001756.1:CDS
AACTACTGCGATTGTTTCAC+CGG 0.429923 1:+25845790 None:intergenic
CTCATGATCCACATGTTGTT+AGG 0.436119 1:-25845583 MsaT001756.1:intron
GAAACCTCGCGAAGCGTAGT+AGG 0.445811 1:+25845623 None:intergenic
AGAGCAAGAGAGAAAGCTAC+TGG 0.475204 1:-25845701 MsaT001756.1:CDS
TTCGAGAAGCTCGAGAAAGT+TGG 0.501777 1:-25845749 MsaT001756.1:CDS
TCCTCCTACTACGCTTCGCG+AGG 0.556697 1:-25845627 MsaT001756.1:CDS
GAGAAGCTCGAGAAAGTTGG+AGG 0.563232 1:-25845746 MsaT001756.1:CDS
AATTAAGGACAGAACAAGGA+AGG 0.564998 1:-25845456 MsaT001756.1:CDS
CGTCTTCATGAAGATGATGA+AGG 0.573237 1:-25845653 MsaT001756.1:CDS
ACCTCGCGAAGCGTAGTAGG+AGG 0.577549 1:+25845626 None:intergenic
AAGCTCGAGAAAGTTGGAGG+AGG 0.601692 1:-25845743 MsaT001756.1:CDS
TTAACTTACCTAACAACATG+TGG 0.608876 1:+25845575 None:intergenic
ATTAAGGACAGAACAAGGAA+GGG 0.628262 1:-25845455 MsaT001756.1:CDS
GAGCAAGAGAGAAAGCTACT+GGG 0.629277 1:-25845700 MsaT001756.1:CDS
TGGAGGAGGAACGTACGAGA+AGG 0.635343 1:-25845729 MsaT001756.1:CDS
AAACAATTAAGGACAGAACA+AGG 0.651168 1:-25845460 MsaT001756.1:CDS
GATTCAATCATGGAGAAACC+CGG 0.652544 1:-25845809 None:intergenic
ACTACTGCGATTGTTTCACC+GGG 0.654995 1:+25845791 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr1 gene 25845429 25845822 25845429 ID=MsaG001756
Chr1 mRNA 25845429 25845822 25845429 ID=MsaT001756.1;Parent=MsaG001756
Chr1 exon 25845429 25845495 25845429 ID=MsaT001756.1.exon2;Parent=MsaT001756.1
Chr1 CDS 25845429 25845495 25845429 ID=cds.MsaT001756.1;Parent=MsaT001756.1
Chr1 exon 25845584 25845822 25845584 ID=MsaT001756.1.exon1;Parent=MsaT001756.1
Chr1 CDS 25845584 25845822 25845584 ID=cds.MsaT001756.1;Parent=MsaT001756.1
Gene Sequence

>MsaT001756.1

ATGGAGAAACCCGGTGAAACAATCGCAGTAGTTTTATCGGCGAAGAAAGCATTCGAGAAGCTCGAGAAAGTTGGAGGAGGAACGTACGAGAAGGTTTACAGAGCAAGAGAGAAAGCTACTGGGAAGATTGTTGCGTTGAAGAAGACTCGTCTTCATGAAGATGATGAAGGTGTTCCTCCTACTACGCTTCGCGAGGTTTCGATTTTGCGTATGCTCTCTCATGATCCACATGTTGTTAGGTTGTTGGATGTGAAACAATTAAGGACAGAACAAGGAAGGGATAACTGTGTTGTATTTGGTTTTTGA

Protein sequence

>MsaT001756.1

MEKPGETIAVVLSAKKAFEKLEKVGGGTYEKVYRAREKATGKIVALKKTRLHEDDEGVPPTTLREVSILRMLSHDPHVVRLLDVKQLRTEQGRDNCVVFGF*