Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG001831 | AES60398.2 | 85.821 | 134 | 19 | 0 | 1 | 134 | 1 | 134 | 6.72e-72 | 221 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG001831 | tr|G7I6L4|G7I6L4_MEDTR | 85.821 | 134 | 19 | 0 | 1 | 134 | 1 | 134 | 3.21e-72 | 221 |
| Gene ID | Type | Classification |
|---|---|---|
| MsaG001831 | TF | C2C2-GATA |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG001831 | MtrunA17_Chr5g0399781 | 51.852 | 54 | 25 | 1 | 3 | 56 | 12 | 64 | 1.33e-11 | 60.1 |
| MsaG001831 | MtrunA17_Chr4g0055611 | 66.667 | 36 | 12 | 0 | 3 | 38 | 12 | 47 | 6.04e-11 | 58.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 20 sgRNAs with CRISPR-Local
Find sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AACAAAAGCTTCAAAGTCTA+TGG | 0.184389 | 1:+27387646 | MsaT001831.1:CDS |
| GAGCTTTCAAACCAATTTCC+AGG | 0.378813 | 1:+27387329 | MsaT001831.1:CDS |
| TGGAAATTGGTTTGAAAGCT+CGG | 0.417197 | 1:-27387327 | None:intergenic |
| CCACAAGCATTACATAGTAT+TGG | 0.433061 | 1:-27387200 | None:intergenic |
| GAAACTAAACTTTGGCGTAA+AGG | 0.456777 | 1:+27387164 | MsaT001831.1:CDS |
| TGCTTGTGGACTACATTTCA+AGG | 0.461822 | 1:+27387214 | MsaT001831.1:CDS |
| ATCAGACCTTGACGTTGAAA+AGG | 0.465983 | 1:+27387289 | MsaT001831.1:CDS |
| GGACTACATTTCAAGGCTAA+AGG | 0.474721 | 1:+27387221 | MsaT001831.1:CDS |
| TCTTCGGAAGATAGTTATCA+AGG | 0.478433 | 1:-27387247 | None:intergenic |
| TGTATACCAAGCAATGAGAT+AGG | 0.478624 | 1:+27387735 | MsaT001831.1:CDS |
| GGAAATTGGTTTGAAAGCTC+GGG | 0.487200 | 1:-27387326 | None:intergenic |
| ATCGATTGTGAAGAATCAAA+TGG | 0.491491 | 1:+27387614 | MsaT001831.1:CDS |
| CATAGTATTGGCTTCTCATA+CGG | 0.492997 | 1:-27387188 | None:intergenic |
| CCAAGCAATGAGATAGGCAT+TGG | 0.496933 | 1:+27387741 | MsaT001831.1:CDS |
| ATTTGATTCTTCACAATCGA+TGG | 0.523804 | 1:-27387612 | None:intergenic |
| ACAAAAGCTTCAAAGTCTAT+GGG | 0.556384 | 1:+27387647 | MsaT001831.1:CDS |
| CCAATGCCTATCTCATTGCT+TGG | 0.566560 | 1:-27387741 | None:intergenic |
| CCAATACTATGTAATGCTTG+TGG | 0.579274 | 1:+27387200 | MsaT001831.1:CDS |
| CTTCGGAAGATAGTTATCAA+GGG | 0.629882 | 1:-27387246 | None:intergenic |
| GATTGTGAAGAATCAAATGG+AGG | 0.680011 | 1:+27387617 | MsaT001831.1:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr1 | gene | 27387152 | 27387809 | 27387152 | ID=MsaG001831 |
| Chr1 | mRNA | 27387152 | 27387809 | 27387152 | ID=MsaT001831.1;Parent=MsaG001831 |
| Chr1 | exon | 27387152 | 27387350 | 27387152 | ID=MsaT001831.1.exon1;Parent=MsaT001831.1 |
| Chr1 | CDS | 27387152 | 27387350 | 27387152 | ID=cds.MsaT001831.1;Parent=MsaT001831.1 |
| Chr1 | exon | 27387604 | 27387809 | 27387604 | ID=MsaT001831.1.exon2;Parent=MsaT001831.1 |
| Chr1 | CDS | 27387604 | 27387809 | 27387604 | ID=cds.MsaT001831.1;Parent=MsaT001831.1 |
| Gene Sequence |
| Protein sequence |
