AlfalfaGEDB | MsaG001844

M. sativa ssp. caerulea / MsaG001844

KEGG: -
GO: -
PFAM: PF02365.18

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG001844	XP_003590172.1	88.146	329	35	1	1	329	1	325	0.0	590

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG001844	sp\|Q9FY93\|NAC83_ARATH	33.562	146	82	6	13	154	22	156	7.61e-13	70.9

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG001844	tr\|G7I6N9\|G7I6N9_MEDTR	88.146	329	35	1	1	329	1	325	0.0	590

TFs/TRs:

Gene ID	Type	Classification
MsaG001844	TF	NAC

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsaG041905	MsaG001844	0.815579	1.490002e-51	2.922780e-49
MsaG045291	MsaG001844	0.804166	4.150708e-49	6.172215e-47
MsaG001844	MsaG004535	0.994180	3.196822e-204	6.677447e-197
MsaG001844	MsaG033321	0.803389	6.006680e-49	8.772822e-47
MsaG017173	MsaG001844	0.821073	8.605939e-53	1.947286e-50
MsaG018834	MsaG001844	0.838909	4.001772e-57	1.509810e-54
MsaG028001	MsaG001844	0.807122	1.001322e-49	1.595638e-47
MsaG029540	MsaG001844	0.825300	8.969478e-54	2.274956e-51
MsaG029951	MsaG001844	0.837242	1.069535e-56	3.832450e-54

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG001844	MtrunA17_Chr1g0168571	88.146	329	35	1	1	329	1	325	0.0	590
MsaG001844	MtrunA17_Chr1g0174911	54.728	349	110	11	1	328	1	322	7.00e-106	312
MsaG001844	MtrunA17_Chr7g0274971	62.051	195	62	2	1	183	1	195	8.57e-80	242
MsaG001844	MtrunA17_Chr7g0263931	32.903	155	85	6	13	157	19	164	1.33e-15	76.6
MsaG001844	MtrunA17_Chr7g0214071	31.944	144	86	4	13	154	23	156	4.55e-15	73.9
MsaG001844	MtrunA17_Chr7g0261411	29.861	144	90	5	13	154	18	152	4.60e-11	62.8
MsaG001844	MtrunA17_Chr5g0417791	30.719	153	89	6	13	157	11	154	5.47e-11	62.0

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG001844	AT5G13180.1	33.562	146	82	6	13	154	22	156	7.73e-14	70.9
MsaG001844	AT5G62380.2	32.680	153	94	6	13	162	15	161	5.29e-13	69.3
MsaG001844	AT5G62380.1	32.680	153	94	6	13	162	15	161	5.29e-13	69.3
MsaG001844	AT3G10480.2	32.895	152	88	8	13	158	35	178	8.62e-13	69.3
MsaG001844	AT1G56010.2	27.551	196	116	10	13	198	27	206	3.49e-12	66.6
MsaG001844	AT3G10480.1	32.680	153	88	8	13	158	35	179	6.31e-12	66.6
MsaG001844	AT1G71930.2	31.544	149	91	7	13	157	17	158	7.13e-12	65.9
MsaG001844	AT1G71930.1	31.544	149	91	7	13	157	17	158	7.13e-12	65.9
MsaG001844	AT2G24430.2	29.730	148	94	5	13	157	24	164	1.01e-11	65.5
MsaG001844	AT2G24430.1	29.730	148	94	5	13	157	24	164	1.01e-11	65.5
MsaG001844	AT5G63790.1	32.919	161	89	6	3	154	48	198	4.73e-11	63.2
MsaG001844	AT5G63790.2	32.919	161	89	6	3	154	58	208	6.04e-11	63.2

Find 60 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AGTAGCAGATTTCTTCTTTA+AGG	0.123640	1:+27586903	None:intergenic
CCATTGTTAAGTTGATTATT+TGG	0.177690	1:+27585391	None:intergenic
CAAAGAATCCATGGGAGATT+TGG	0.252767	1:-27586981	MsaT001844.1:CDS
TCTGCATAGAACATAATTAT+TGG	0.253885	1:+27586592	None:intergenic
TAAGTTGATTATTTGGATTT+TGG	0.278659	1:+27585398	None:intergenic
CCTCAACTCTTCTTCCAATA+AGG	0.285996	1:-27587115	MsaT001844.1:CDS
GCACTATTGGACTTGGTAGA+TGG	0.308744	1:-27586864	MsaT001844.1:CDS
AAGTCTCAACGTAAGGAAAA+AGG	0.316342	1:-27585942	MsaT001844.1:CDS
CATCTACTTCTTTGCTTGTA+AGG	0.319920	1:+27585338	None:intergenic
ATGGTGATTGCCGCAGGTTC+AGG	0.327042	1:+27586494	None:intergenic
CAGAACAATAATGGTTTGTT+GGG	0.343371	1:-27585433	MsaT001844.1:CDS
ACATGTAGCACTTACAGATT+TGG	0.365447	1:+27585866	None:intergenic
AGTGGGTGCGATAACCTTAT+TGG	0.365716	1:+27587101	None:intergenic
AAAGAATCCATGGGAGATTT+GGG	0.379921	1:-27586980	MsaT001844.1:CDS
TTCATTGATAAGCAATCCTT+TGG	0.384426	1:-27586704	MsaT001844.1:intron
TCACGCGTAGTTCGCACTAT+TGG	0.388612	1:-27586877	MsaT001844.1:CDS
GCAGAACAATAATGGTTTGT+TGG	0.393833	1:-27585434	MsaT001844.1:CDS
ATATTGTAGCAGAACAATAA+TGG	0.395561	1:-27585442	MsaT001844.1:intron
TTAAGGTTGTAAAGAAGTAT+AGG	0.426160	1:+27586920	None:intergenic
GCAAGATCCATCCACCATCT+TGG	0.430936	1:+27586746	None:intergenic
CAAGATCCATCCACCATCTT+GGG	0.431083	1:+27586747	None:intergenic
ATTCTTGAATATGATTTGTT+CGG	0.436097	1:-27587009	MsaT001844.1:CDS
AGATGGGAAGGTGAAGATGC+TGG	0.438944	1:-27586847	MsaT001844.1:CDS
TTCAAGAATAGTGATATGAT+TGG	0.441569	1:+27587023	None:intergenic
AAAAGGAAAATATGTGGAAT+GGG	0.469749	1:-27585925	MsaT001844.1:CDS
CTTACAAGCAAAGAAGTAGA+TGG	0.471112	1:-27585337	MsaT001844.1:CDS
ATCTACTTCTTTGCTTGTAA+GGG	0.474103	1:+27585339	None:intergenic
TGGGAGATTTGGGAAGAGTT+CGG	0.477611	1:-27586970	MsaT001844.1:CDS
CTTATTGGAAGAAGAGTTGA+GGG	0.477892	1:+27587116	None:intergenic
CACTATTGGACTTGGTAGAT+GGG	0.484248	1:-27586863	MsaT001844.1:CDS
AATCTCCCATGGATTCTTTG+CGG	0.489258	1:+27586984	None:intergenic
TCAAGAATAGTGATATGATT+GGG	0.493343	1:+27587024	None:intergenic
ACAGGTATGGTGATTGCCGC+AGG	0.519451	1:+27586488	None:intergenic
AAAGTAGTAACTTACAGGTA+TGG	0.522367	1:+27586475	None:intergenic
AACTCTTCCCAAATCTCCCA+TGG	0.530624	1:+27586973	None:intergenic
GATGGGAAGGTGAAGATGCT+GGG	0.538585	1:-27586846	MsaT001844.1:CDS
TCGGCACCGCAAAGAATCCA+TGG	0.544845	1:-27586990	MsaT001844.1:CDS
TTGTAGAAGTTCATTATCAG+TGG	0.559795	1:+27587083	None:intergenic
AAGAGTGACACTTCCCAAGA+TGG	0.562341	1:-27586760	MsaT001844.1:CDS
CCAAATAATCAACTTAACAA+TGG	0.564212	1:-27585391	MsaT001844.1:CDS
GATTGCTTATCAATGAATTG+TGG	0.566276	1:+27586710	None:intergenic
TGTAGAAGTTCATTATCAGT+GGG	0.573117	1:+27587084	None:intergenic
ACACTTCCCAAGATGGTGGA+TGG	0.575047	1:-27586753	MsaT001844.1:CDS
TAATGCAAAGTCTCAACGTA+AGG	0.575719	1:-27585949	MsaT001844.1:CDS
ATGATGATGCTAGTATGTCT+TGG	0.583925	1:-27585306	MsaT001844.1:CDS
TGCTTATCAATGAATTGTGG+AGG	0.585480	1:+27586713	None:intergenic
AGAACAATAATGGTTTGTTG+GGG	0.592459	1:-27585432	MsaT001844.1:CDS
AGCTGCAATGAAAACAACTC+AGG	0.594475	1:+27585283	None:intergenic
CCTTATTGGAAGAAGAGTTG+AGG	0.596076	1:+27587115	None:intergenic
ATTGGACTTGGTAGATGGGA+AGG	0.599219	1:-27586859	MsaT001844.1:CDS
ACTTTGCATTAACAACAGTA+CGG	0.599369	1:+27585961	None:intergenic
ATAGTGATATGATTGGGCAT+AGG	0.602973	1:+27587030	None:intergenic
GTAGTTCGCACTATTGGACT+TGG	0.612695	1:-27586871	MsaT001844.1:CDS
TGAAGGATGCTTGATCGAAG+AGG	0.615586	1:-27585248	MsaT001844.1:CDS
CGGCACCGCAAAGAATCCAT+GGG	0.621679	1:-27586989	MsaT001844.1:CDS
AGTGACACTTCCCAAGATGG+TGG	0.639769	1:-27586757	MsaT001844.1:CDS
GGAACTTCCTACTCGTATCG+TGG	0.641732	1:-27586949	MsaT001844.1:CDS
GTAGCACTTACAGATTTGGT+CGG	0.664478	1:+27585870	None:intergenic
GACACTCGTTCCTGAACCTG+CGG	0.677063	1:-27586504	MsaT001844.1:CDS
GAACAATAATGGTTTGTTGG+GGG	0.677487	1:-27585431	MsaT001844.1:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content

Chromosome	Type	Strat	End	Strand	Name
Chr1	gene	27585180	27587142	27585180	ID=MsaG001844
Chr1	mRNA	27585180	27587142	27585180	ID=MsaT001844.1;Parent=MsaG001844
Chr1	exon	27585180	27585455	27585180	ID=MsaT001844.1.exon4;Parent=MsaT001844.1
Chr1	CDS	27585180	27585455	27585180	ID=cds.MsaT001844.1;Parent=MsaT001844.1
Chr1	exon	27585881	27586030	27585881	ID=MsaT001844.1.exon3;Parent=MsaT001844.1
Chr1	CDS	27585881	27586030	27585881	ID=cds.MsaT001844.1;Parent=MsaT001844.1
Chr1	exon	27586490	27586615	27586490	ID=MsaT001844.1.exon2;Parent=MsaT001844.1
Chr1	CDS	27586490	27586615	27586490	ID=cds.MsaT001844.1;Parent=MsaT001844.1
Chr1	exon	27586705	27587142	27586705	ID=MsaT001844.1.exon1;Parent=MsaT001844.1
Chr1	CDS	27586705	27587142	27586705	ID=cds.MsaT001844.1;Parent=MsaT001844.1

Gene Sequence

>MsaT001844.1

ATGGCCCTCAACTCTTCTTCCAATAAGGTTATCGCACCCACTGATAATGAACTTCTACAACAATTCCTCTACAACAAAATTCACAACAAACCTATGCCCAATCATATCACTATTCTTGAATATGATTTGTTCGGCACCGCAAAGAATCCATGGGAGATTTGGGAAGAGTTCGGAACTTCCTACTCGTATCGTGGAAAAGACCTATACTTCTTTACAACCTTAAAGAAGAAATCTGCTACTAGCTCACGCGTAGTTCGCACTATTGGACTTGGTAGATGGGAAGGTGAAGATGCTGGGAAAAATATATTGAGCAAAAACAAGAACCAAATCCTCGGCATGAAAAAACGTTTTCGCTTTGAGAAGAGTGACACTTCCCAAGATGGTGGATGGATCTTGCATGAATTTAGCCTCCACAATTCATTGATAAGCAATCCTTTGGCCAATAATTATGTTCTATGCAGATTTAGAAAGAATTGCACTCACTTACCTGTAACCAAAAATATTGTTGCACAAGATAAACTAAGAAAGACACTCGTTCCTGAACCTGCGGCAATCACCATACCTGGCAAGAGAAAATATATAATTTATGAAGAACCAACAAAAGAATCAAACCGTACTGTTGTTAATGCAAAGTCTCAACGTAAGGAAAAAGGAAAATATGTGGAATGGGCAGAAGAGAACTTTGTTCTTGCACTCGAAGCACCGACCAAATCTCAGAACAATAATGGTTTGTTGGGGGAAGATATTGACCAAAATCCAAATAATCAACTTAACAATGGAAAAAACAAGATTGAAGATGATGGTGATTCCCTTACAAGCAAAGAAGTAGATGGCGATGAAGATGATGATGCTAGTATGTCTTGGCCTGAGTTGTTTTCATTGCAGCTGAGAAAAATGAATGAAGGATGCTTGATCGAAGAGGAAGATCTTCCAATGATTTCCAATCATTTTTATAAGGACTTATTGTCGGAAAATATTAATATAAATTAA

Protein sequence

>MsaT001844.1

MALNSSSNKVIAPTDNELLQQFLYNKIHNKPMPNHITILEYDLFGTAKNPWEIWEEFGTSYSYRGKDLYFFTTLKKKSATSSRVVRTIGLGRWEGEDAGKNILSKNKNQILGMKKRFRFEKSDTSQDGGWILHEFSLHNSLISNPLANNYVLCRFRKNCTHLPVTKNIVAQDKLRKTLVPEPAAITIPGKRKYIIYEEPTKESNRTVVNAKSQRKEKGKYVEWAEENFVLALEAPTKSQNNNGLLGEDIDQNPNNQLNNGKNKIEDDGDSLTSKEVDGDEDDDASMSWPELFSLQLRKMNEGCLIEEEDLPMISNHFYKDLLSENININ*