AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG001847


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG001847 MtrunA17_Chr1g0168571 88.308 325 32 1 1 319 1 325 0.0 577
MsaG001847 MtrunA17_Chr1g0174911 54.366 355 92 11 1 318 1 322 3.13e-107 315
MsaG001847 MtrunA17_Chr7g0274971 61.538 195 63 1 1 183 1 195 9.03e-81 244
MsaG001847 MtrunA17_Chr7g0263931 32.903 155 85 6 13 157 19 164 8.74e-15 73.9
MsaG001847 MtrunA17_Chr7g0214071 32.639 144 85 4 13 154 23 156 1.28e-14 72.4
MsaG001847 MtrunA17_Chr4g0018391 31.788 151 94 6 13 160 15 159 1.50e-13 70.5
MsaG001847 MtrunA17_Chr3g0139331 26.486 185 120 6 13 188 19 196 1.08e-11 65.1
MsaG001847 MtrunA17_Chr5g0398971 34.437 151 86 8 13 157 15 158 1.41e-11 64.7
MsaG001847 MtrunA17_Chr8g0384721 35.333 150 79 6 13 154 15 154 1.42e-11 63.9
MsaG001847 MtrunA17_Chr5g0417791 31.373 153 88 6 13 157 11 154 4.16e-11 62.4
MsaG001847 MtrunA17_Chr6g0481091 31.210 157 94 8 13 162 14 163 6.98e-11 61.6
MsaG001847 MtrunA17_Chr1g0147421 30.667 150 95 4 13 159 35 178 7.46e-11 62.4
MsaG001847 MtrunA17_Chr8g0364791 30.065 153 93 8 11 157 12 156 7.49e-11 62.8
MsaG001847 MtrunA17_Chr3g0109821 31.613 155 90 7 13 160 14 159 7.85e-11 63.2
MsaG001847 MtrunA17_Chr8g0384201 30.065 153 93 8 11 157 12 156 9.37e-11 62.4
MsaG001847 MtrunA17_Chr7g0261411 28.472 144 92 5 13 154 18 152 9.73e-11 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG001847 AT5G62380.2 32.026 153 95 6 13 162 15 161 3.85e-14 72.8
MsaG001847 AT5G62380.1 32.026 153 95 6 13 162 15 161 3.85e-14 72.8
MsaG001847 AT2G24430.2 31.544 149 91 6 13 157 24 165 2.32e-13 70.1
MsaG001847 AT2G24430.1 31.544 149 91 6 13 157 24 165 2.32e-13 70.1
MsaG001847 AT5G13180.1 33.793 145 83 5 13 154 22 156 2.74e-13 68.9
MsaG001847 AT3G10480.2 33.121 157 91 8 13 163 35 183 4.84e-13 69.7
MsaG001847 AT5G08790.1 32.620 187 103 8 3 179 5 178 9.27e-13 67.8
MsaG001847 AT3G10480.1 32.911 158 91 8 13 163 35 184 3.08e-12 67.4
MsaG001847 AT5G63790.1 33.540 161 88 6 3 154 48 198 1.44e-11 64.7
MsaG001847 AT5G63790.2 33.540 161 88 6 3 154 58 208 1.52e-11 64.7
MsaG001847 AT5G66300.1 29.747 158 102 6 13 167 20 171 2.13e-11 63.9
MsaG001847 AT3G17730.1 30.061 163 100 8 13 168 14 169 3.30e-11 62.8

Find 64 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGTAGCAGATTTCTTCTTTA+AGG 0.123640 1:+27617352 None:intergenic
AACAATAAGTCCTTATAAAA+TGG 0.162355 1:+27615828 None:intergenic
CCTCAATTCTTCTTCCAATA+AGG 0.222490 1:-27617564 MsaT001847.1:CDS
TTCAAGAATGGTGATATTAT+TGG 0.242234 1:+27617472 None:intergenic
CAAAGAATCCATGGGAGATT+TGG 0.252767 1:-27617430 MsaT001847.1:CDS
TCTGCATAGAACATAATTAT+TGG 0.253885 1:+27617024 None:intergenic
TGTGGGTGCGATAACCTTAT+TGG 0.289405 1:+27617550 None:intergenic
TAAGTTGATTACTTGGATTT+TGG 0.305325 1:+27615994 None:intergenic
GCACTATTGGACTTGGTAGA+TGG 0.308744 1:-27617313 MsaT001847.1:CDS
CCAGGAACGATTGTCATTCT+TGG 0.309932 1:+27616944 None:intergenic
TAAAATGGATTGGAAATCAT+TGG 0.335007 1:+27615843 None:intergenic
TGGGAGATTTGGGAAGAGTT+TGG 0.336128 1:-27617419 MsaT001847.1:CDS
GCAAGAACAAAGTTCTCTTT+TGG 0.336878 1:+27616385 None:intergenic
CCATTGTTAAGTTGATTACT+TGG 0.345697 1:+27615987 None:intergenic
ATATGTAGCACTTACAGATT+TGG 0.368326 1:+27616348 None:intergenic
AAAGAATCCATGGGAGATTT+GGG 0.379921 1:-27617429 MsaT001847.1:CDS
TCACGCGTAGTTCGCACTAT+TGG 0.388612 1:-27617326 MsaT001847.1:CDS
TATAGGAGTACAATAATGTT+TGG 0.388726 1:-27616034 MsaT001847.1:intron
GAGTACAATAATGTTTGGTT+GGG 0.392823 1:-27616029 MsaT001847.1:CDS
TTCTTTGATAAGCAATCCTT+TGG 0.396079 1:-27617153 MsaT001847.1:intron
CCAAGAATGACAATCGTTCC+TGG 0.400269 1:-27616944 MsaT001847.1:CDS
TAAAATTCATAACAAACCTT+TGG 0.414530 1:-27617495 MsaT001847.1:CDS
GGAGTACAATAATGTTTGGT+TGG 0.416706 1:-27616030 MsaT001847.1:CDS
TTAAGGTTGTAAAGAAGTAT+AGG 0.426160 1:+27617369 None:intergenic
GCAAGATCCATCCACCATCT+TGG 0.430936 1:+27617195 None:intergenic
CAAGATCCATCCACCATCTT+GGG 0.431083 1:+27617196 None:intergenic
ATTCTTGAATACGATTTGTT+CGG 0.433061 1:-27617458 MsaT001847.1:CDS
ACAGTGATTGCGGCAGGTCC+AGG 0.448826 1:+27616926 None:intergenic
CTTATTGGAAGAAGAATTGA+GGG 0.451784 1:+27617565 None:intergenic
TAAGTCCTTATAAAATGGAT+TGG 0.478264 1:+27615833 None:intergenic
CACTATTGGACTTGGTAGAT+GGG 0.484248 1:-27617312 MsaT001847.1:CDS
AATCTCCCATGGATTCTTTG+CGG 0.489258 1:+27617433 None:intergenic
AGTACAATAATGTTTGGTTG+GGG 0.510246 1:-27616028 MsaT001847.1:CDS
CCTTATTGGAAGAAGAATTG+AGG 0.526520 1:+27617564 None:intergenic
AACTCTTCCCAAATCTCCCA+TGG 0.530624 1:+27617422 None:intergenic
GAACAAATCGTATTCAAGAA+TGG 0.535727 1:+27617460 None:intergenic
TGAGACTTTGTATTAACCAC+AGG 0.541279 1:+27616439 None:intergenic
TGTAGCACTTACAGATTTGG+TGG 0.543882 1:+27616351 None:intergenic
TCGGCACCGCAAAGAATCCA+TGG 0.544845 1:-27617439 MsaT001847.1:CDS
GATTGCTTATCAAAGAATTG+TGG 0.559456 1:+27617159 None:intergenic
AAGAGTGACACTTCCCAAGA+TGG 0.562341 1:-27617209 MsaT001847.1:CDS
TAATACAAAGTCTCAACGTA+AGG 0.571382 1:-27616431 MsaT001847.1:CDS
ACACTTCCCAAGATGGTGGA+TGG 0.575047 1:-27617202 MsaT001847.1:CDS
TTGTAGAAGTTCATCATCTG+TGG 0.575778 1:+27617532 None:intergenic
ATGATGATGCTAGTATGTCT+TGG 0.583925 1:-27615932 MsaT001847.1:CDS
ATGGTGATATTATTGGCCAA+AGG 0.586567 1:+27617479 None:intergenic
TGCTTATCAAAGAATTGTGG+AGG 0.589131 1:+27617162 None:intergenic
ACAAGTACAGTGATTGCGGC+AGG 0.590813 1:+27616920 None:intergenic
CCAAGTAATCAACTTAACAA+TGG 0.597996 1:-27615987 MsaT001847.1:CDS
ATTGGACTTGGTAGATGGGA+AGG 0.599219 1:-27617308 MsaT001847.1:CDS
AGCTGCAACGAAAACAACTC+AGG 0.602909 1:+27615909 None:intergenic
AAAGACAAGAACCAAATCCT+CGG 0.605189 1:-27617257 MsaT001847.1:CDS
TGTAGAAGTTCATCATCTGT+GGG 0.611308 1:+27617533 None:intergenic
GTAGTTCGCACTATTGGACT+TGG 0.612695 1:-27617320 MsaT001847.1:CDS
CGGCACCGCAAAGAATCCAT+GGG 0.621679 1:-27617438 MsaT001847.1:CDS
AGATGGGAAGGTGAAGACGC+TGG 0.631377 1:-27617296 MsaT001847.1:CDS
AGTGACACTTCCCAAGATGG+TGG 0.639769 1:-27617206 MsaT001847.1:CDS
GTAGCACTTACAGATTTGGT+GGG 0.644008 1:+27616352 None:intergenic
ACTTACAAGTACAGTGATTG+CGG 0.645359 1:+27616916 None:intergenic
GGAGCTTCTCACTCATATCG+TGG 0.649333 1:-27617398 MsaT001847.1:CDS
GTACAATAATGTTTGGTTGG+GGG 0.656762 1:-27616027 MsaT001847.1:CDS
AAAAGAATCAAACTGTCCTG+TGG 0.676178 1:-27616455 MsaT001847.1:CDS
CAGCTGAGAGAGATGTGTGA+AGG 0.679441 1:-27615891 MsaT001847.1:CDS
TGAAGGATGCTTGATCGAAG+AGG 0.711175 1:-27615874 MsaT001847.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr1 gene 27615806 27617591 27615806 ID=MsaG001847
Chr1 mRNA 27615806 27617591 27615806 ID=MsaT001847.1;Parent=MsaG001847
Chr1 exon 27615806 27616051 27615806 ID=MsaT001847.1.exon4;Parent=MsaT001847.1
Chr1 CDS 27615806 27616051 27615806 ID=cds.MsaT001847.1;Parent=MsaT001847.1
Chr1 exon 27616363 27616512 27616363 ID=MsaT001847.1.exon3;Parent=MsaT001847.1
Chr1 CDS 27616363 27616512 27616363 ID=cds.MsaT001847.1;Parent=MsaT001847.1
Chr1 exon 27616922 27617047 27616922 ID=MsaT001847.1.exon2;Parent=MsaT001847.1
Chr1 CDS 27616922 27617047 27616922 ID=cds.MsaT001847.1;Parent=MsaT001847.1
Chr1 exon 27617154 27617591 27617154 ID=MsaT001847.1.exon1;Parent=MsaT001847.1
Chr1 CDS 27617154 27617591 27617154 ID=cds.MsaT001847.1;Parent=MsaT001847.1
Gene Sequence

>MsaT001847.1

ATGGCCCTCAATTCTTCTTCCAATAAGGTTATCGCACCCACAGATGATGAACTTCTACAACAATTCCTCTACAATAAAATTCATAACAAACCTTTGGCCAATAATATCACCATTCTTGAATACGATTTGTTCGGCACCGCAAAGAATCCATGGGAGATTTGGGAAGAGTTTGGAGCTTCTCACTCATATCGTGGAAAAGACCTATACTTCTTTACAACCTTAAAGAAGAAATCTGCTACTAGCTCACGCGTAGTTCGCACTATTGGACTTGGTAGATGGGAAGGTGAAGACGCTGGAAAAAATATATTCAGCAAAGACAAGAACCAAATCCTCGGCATGAAAAAACGTTTTCGCTTTGAGAAGAGTGACACTTCCCAAGATGGTGGATGGATCTTGCATGAATATAGCCTCCACAATTCTTTGATAAGCAATCCTTTGGCCAATAATTATGTTCTATGCAGATTTAGAAAGAATTGCACTGACTTACCTGTAAGCAAAAATATTGTTGCACAAGATAAACCAAGAATGACAATCGTTCCTGGACCTGCCGCAATCACTGTACTTGGCAAGAGAAAATATATAATCTATGAAGAACCAACAAAAGAATCAAACTGTCCTGTGGTTAATACAAAGTCTCAACGTAAGGAAAAAGAAAAATATGTGGAATGGCCAAAAGAGAACTTTGTTCTTGCACTCGAAGCACCCACCAAATCTGAGTACAATAATGTTTGGTTGGGGGAAGATATTGACCAAAATCCAAGTAATCAACTTAACAATGGAAAAAACAAGATTGAAGTAGATGGCGATGAAGATGATGATGCTAGTATGTCTTGGCCTGAGTTGTTTTCGTTGCAGCTGAGAGAGATGTGTGAAGGATGCTTGATCGAAGAGGAAGATGTTCCAATGATTTCCAATCCATTTTATAAGGACTTATTGTTGGAAAATATTAATATAAATTAA

Protein sequence

>MsaT001847.1

MALNSSSNKVIAPTDDELLQQFLYNKIHNKPLANNITILEYDLFGTAKNPWEIWEEFGASHSYRGKDLYFFTTLKKKSATSSRVVRTIGLGRWEGEDAGKNIFSKDKNQILGMKKRFRFEKSDTSQDGGWILHEYSLHNSLISNPLANNYVLCRFRKNCTDLPVSKNIVAQDKPRMTIVPGPAAITVLGKRKYIIYEEPTKESNCPVVNTKSQRKEKEKYVEWPKENFVLALEAPTKSEYNNVWLGEDIDQNPSNQLNNGKNKIEVDGDEDDDASMSWPELFSLQLREMCEGCLIEEEDVPMISNPFYKDLLLENININ*